Medicine and Health Sciences Commons^™

A Mouse Model For Evaluating The Induction Of Liver Glucose-6- Phosphate Dehydrogenase By Halothane, Ergül Belge Kurutaş, İlhan Tuncer

Turkish Journal of Veterinary & Animal Sciences

The effect of halothane anesthesia in different doses and a period of two weeks on glucose-6-phosphate dehydrogenase (G6PDH) activity of mouse liver were investigated. The mean and SD of G6PDH for liver of normal mice were 0.036±0.017 U/mg protein and 1.756±0.814 U/g liver, respectively. Although there was histopathological damage in the liver, liver weights in treated mice increased significantly compared to control animals. Increasing the dosage of halothane inhalation induced liver G6PDH enzyme activity (U/g liver) by 84%, 42% and 4%, showing an inverse relation with the dose of halothane (0.25, 0.50, 1.00 cc twice daily for two weeks). Although …

The Monthly Variations In The Blood Glucose Level Of Capoeta Capoeta Capoeta (G.Ldenstaedt, 1772) Living In Aras River, Seyi̇t Aydin, Ayhan Yildirim, Orhan Erdoğan

Turkish Journal of Veterinary & Animal Sciences

In this study, the monthly variation in the blood glucose level of Capoeta capoeta capoeta caught from Aras between August 1995 and June 1996 was determined. The average blood glucose level was determined to be 112.04±5.19 mg/dl in males and 106.32±9.68 mg/dl in females and 110.06±4.19 mg/dl in the population. The blood glucose level was highest (141.07±13.67 mg/dl) in April, and lowest (69.14±9.75 mg/dl) in September. It was deter- mined that the level of blood glucose was related to temperature and reproduction.

Effect Of Lighting On Growth Performance And Carcass Characteristics Of Bronze Turkeys Reared In Intensive Conditions, Turgay Şengül, Yusuf Konca, Ahmet Yildiz

Turkish Journal of Veterinary & Animal Sciences

The present study was designed to determine the effects of lighting regimes on the growth performances and carcass characteristics of Bronze turkeys reared in intensive conditions. Turkeys were reared under three lighting regimes (group 1, 12 h; group 2, 18 h; and group 3, 23 h.). In the experiment, the live weights of the turkeys (male+female) in groups 1, 2 and 3 were found to be 4578±142, 4630±131 and 4942±139 g respectively at the end of a 16-week period. The live weights during the 16-wk period were found to be affected (P0.05). These values were 3347±152, 3389±141 and 3554±197 g; …

Determination Of Anti- Toxoplasma Gondiiantibodies In Cattle In Elazığ And Environs By Sabin-Feldman (Sf) Test, Müni̇r Aktaş, Cahi̇t Babür, K. Zafer Karaer, Nazi̇r Dumanli

Turkish Journal of Veterinary & Animal Sciences

Toxoplasma gondii pseudocysts in cattle are sources of infection for humans. Therefore, cattle toxoplasmosis is a very important factor in public health. The aim of the present study was to determine the seroprevalence of cattle toxoplasmosis. A total of 122 cattle from different age groups were selected in Elazığ and environs. Blood samples were taken and sera were collected. Serum samples were kept at -20^0 C until further examination. The values of toxoplasmosis seroprevalence were determined by SF test for anti-Toxoplasma gondii antibodies. Antibodies were detected in 86 out of 122 cattle sera (70.49%). Fifty-four (62.75%), 26 (30.23%), 6 (6.97%) …

Comparative Studies On The Production Performances Of Chios And Imroz Sheep Kept Under Semi-Intensive Condition Iii. The Effects Of Some Environmental Factors On Production Characteristics, Şebnem Gündal Çörekçi̇, Mehmet Evri̇m

Turkish Journal of Veterinary & Animal Sciences

This paper reports the third part of a study carried out to reveal the comparative production performances and effects of some environmental factors on the production characteristics of Chios and Imroz ewes kept in a single flock under semi-intensive conditions. In this report, the effects of some environmental factors on production characteristics are considered. The effects of genotype, sex (except in the case of fleece qualities) type of parturition, the age of the mother and years of birth on the fleece yields and body weights of lambs at different periods and first-shearing (6 months of age) fleece yields were investigated. …

The Detection Of Mutagenic Activity Of Some Chemicals (Azamethyphos, Dichlorvos, Methyl Parathion, Aflatoxinb1) By Smart Drosophila Melanogaster, Selma Ekebaş, Şükran Çakir, Okan Ertuğrul, Aykut Kence

Turkish Journal of Veterinary & Animal Sciences

In this study mutagenic effects of some chemicals used in Veterinary Medicine and agricultural fields was investigated by Somatic Mutation and Recombination Test (SMART). SMART test was to observe the chemical effects on wing phenotype of the trans- heterozygote flies carrying marker gene. Lethal doses of the chemicals used were determined. A positive correlation was observed between total mutation and the number of wings having mutations. In addition, the observed mutations were classified according to the size and the type of the mutations per wing. Chemicals used were ranked as Dichlorvos, Methyl parathion, Azamethyphos according to their genotoxic effects. The …

Determination Of The Shelf-Life Of Marinated Fish Balls, Candan Varlik, Nuray Erkan, Sühendan Mol, Taçnur Baygar, Özkan Özden

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was the determination of the shelf life of marinated fish balls. The anchovies were boiled, and after bones and heads were removed they were mixed with spice and fried. Then, they were placed in brine and stored at +4^0 C (± 1). The sensory analyses pH, TVB-N and TMA-N analyses were performed every 15 days during the storage. The results showed that this product was excellent quality for 60 days, fair quality for 105 days, and was marketable until the 120^th day. The spoilage was determined after the 120 ^th day.

The Effect Of Water Current On Omega-3 Fatty Acids Of Rainbow Trout (Oncorhynchus Mykiss) Fillets, Mehmet Çeli̇k

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of water current on omega-3 (n-3) fatty acids of trout ( Oncorhynchus mykiss) were investigated. Compared with group B the fish of group A showed a higher content of the individual n-3 fatty acids C18:3 n-3, C18:4 n-3, C20:4 n-3, C20:5 n-3 (p

Applications Of The Atomic Force Microscope Within The Fields Of Haematology And Cytogenetics, Margaret O'Reilly-Crowley

Theses

Atomic force microscopy [AFM] has become a rapidly developing interdisciplinary field of research in recent years. The technique, including instrumentation and specimen preparation methods, is now more sophisticated and its application has increased dramatically. The key advantage of AFM is in providing three-dimensional images of samples imaged. Both size and shape of samples imaged can be obtained allowing their subsequent quantification. Red blood cells [RBCs] and chromosomes were imaged in this work. A brief overview of the current status of biomedical AFM, has been presented in the review.

Measurement of mean cell volume [MCV] of immobilised red blood cells with …

The Molecular Epidemiology Of Rotavirus In Ireland, Fiona O'Halloran

Theses

Between 1997 and 1998, 3,136 cases of rotavirus diarrhoea were detected in Irish children less than 2 years of age. Hospital inpatients accounted for 80% of these infections, with the remainder being diagnosed in general practice. A large percentage of infections were detected in neonates, suggesting a possible inadequate maternal derived protection from existing indigenous strains. This feature suggested the possible existence of ‘novel’ strains circulating in Ireland. No data describing the epidemiology of rotavirus strains in this country currently exists. Furthermore no assessment of the potential health-economic impact or quantitation of potential disease burden was ever undertaken

Three hundred …

Risk Of Imported Filariasis In Pakistan, M A. Beg

Department of Pathology and Laboratory Medicine

No abstract provided.

Investigations Of The Absorption And Metabolismof Antioxidant Flavonols, Abdul Aziz Azlina

Student Works (2000-2009)

Flavonols are polyphenols, secondary plant metabolites commonly found in plants and foods of plant origin. They have widespread biological properties in the human body. The recent discovery of their potential antioxidant activities has prompted extensive research. Flavonols particularly quercetin are potent antioxidants with higher antioxidant properties than the well known antioxidant vitamins C and E. Several epidemiology studies have demonstrated a strong inverse association between flavonoid intake and risk of coronary heart disease. The association with cancer is less defined with only some studies showing an inverse association and others not. In view of their potential to act as antioxidants …

Natural Antibodies To Adrenal Hormones And Apolipoprotein E In Cardiovascular Physiology, Shabana Aafaqi

Student Works (2000-2009)

Natural autoantibodies (NAA} are autoantibodies that are present in the sera of healthy individuals. NAA can belong to the lgA, lgG and lgM isotypcs. They exhibit high degrees of polyreactivity and connectivity but low affinity to self antigens. The main function of NAA in healthy people is probably 10 clear the catabolic waste and ageing cells. In this study, an indirect Enzyme-linked lmmunosorbent Assay (ELISA) method was used to detect NAA against hormones, namely adreno-co11ical (hydrocortisone, aldostcrone, androstcncdionc), adrcno-medullary (adrenaline. noradrenaline) and other related steroid hormones (progesterone, testosterone, P-cstradiol). In eleven normal human serum (NHS), lgG anti-cortisol were delcctcd in …

Detection And Modeling Of Radiation Induced Effects In Tissues By Dielectric Spectroscopy, Dorin A. Todor

Physics Theses & Dissertations

The work presented here is applied physics research in the field of radiation treatment. We address the development of a new and innovative method, in vivo and possibly non-invasive, for tumor and healthy tissues control during and after the radiation treatment. The radiation treatment is delivered in an almost standardized manner for particular classes of tumors. The large variance in the individual radio sensitivity of healthy tissues and tumors often leads to local recurrence of neoplastic growth and/or distant metastatic disease which often remains untreated. The method is based on the measurement and analysis of electrical impedance data in …

Extensive Conservation Of Sex Chromosome Organization Between Cat And Human Revealed By Parallel Radiation Hybrid Mapping, William J. Murphy, Shan Sun, Zhang-Qun Chen, Jill Pecon-Slattery, Stephen J. O'Brien

Biology Faculty Articles

A radiation hybrid (RH)-derived physical map of 25 markers on the feline X chromosome (including 19 Type I coding loci and 6 Type II microsatellite markers) was compared to homologous marker order on the human and mouse X chromosome maps. Complete conservation of synteny and marker order was observed between feline and human X chromosomes, whereas the same markers identified a minimum of seven rearranged syntenic segments between mouse and cat/human X chromosome marker order. Within the blocks, the feline, human, and mouse marker order was strongly conserved. Similarly, Y chromosome locus order was remarkably conserved between cat and human …

The Role Of Brn-3.2 In Retinal Ganglion Cell Differentiation, Jerin Marie Wright

Loma Linda University Electronic Theses, Dissertations & Projects

Within the retina, the POU domain of transcription factors brn-3.0, brn-3.1, and brn-3.2 are present only in retinal ganglion cells. These genes are believed to be involved in establishing neural cell lineages in mammals. In this study brn-3.2 was examined by comparing the number of ganglion cells present during postnatal development in normal mice (+/+), in mice homozygous (-/-) for the brn-3.2 gene, and in adult mice with a heterozygous gene deletion (+/-) for brn-3.2. Optic nerve cross sections were imaged by electron microscopy, and axon profiles counted systematically by hand. These counts were then related to the nerve cross …

A Preclinical Model Of Ionizing Radiation And Tnf-Alpha Gene Therapy For Cancer, Jun Li

Loma Linda University Electronic Theses, Dissertations & Projects

Long term control of high-grade brain tumors is rarely achieved with current therapeutic regimens. This study sought to determine if pretreatment with plasmid DNA expressing Tumor Necrosis Factor-α (TNF-α) could enhance the effect of ionizing radiation in an aggressive, rapidly growing glioma tumor (C6) model. Plasmid DNA is an attractive vector system for delivery of foreign genes into eukaryotic cells due to its advantages including low immunogenicity in vivo, lesser toxicity and relatively low cost. We have constructed a plasmid-based mammalian expression vector (pGL1-TNF) to secrete human TNF-α from cancer cells. Plasmid DNA was successfully transfected into C6 cells …

Method And Apparatus For Controlling Acoustic Signal Bandwidth In An Ultrasonic Diagnostic Imaging System, Jeffrey R. Resnick, Gregory R. Bashford

Biomedical Imaging and Biosignal Analysis Laboratory

An ultrasonic imaging system includes a receive beam former that generates analog receive signals and a scan converter. A receive signal processing path interconnects the receive beamformer and the scan converter, and this processing path includes both an A/D converter characterized by a selectable sampling rate and at least one filter characterized by at least one filter parameter. The filter parameter is selected as a function of the sampling rate to provide enhanced image quality.

Enhanced Apoptosis Mediates Inhibition Of Ebv-Transformed Lymphoblastoid Cell Line Proliferation By Curcumin., D Ranjan, T D Johnston, K S Reddy, G Wu, Subbarao Bondada, C Chen

Microbiology, Immunology, and Molecular Genetics Faculty Publications

BACKGROUND: Epstein-Barr virus (EBV)-associated B-cell lymphomas occur more frequently in immunodeficient states such as organ transplantation and HIV infection. We have previously reported that B cell immortalization with EBV was promoted by cyclosporin A (CyA) and that curcumin (Cur), a natural phenol with known antioxidant and antitumor properties, blocked EBV-induced B cell immortalization. In the following experiments we show that Cur inhibits the proliferation of EBV-transformed lymphoblastoid cell lines (LCL) via enhanced apoptosis.

METHODS: LCL were generated by infecting freshly isolated human B cells with EBV (B95-8) for 12 h and coculturing with predetermined optimal concentrations of CyA (500 ng/ml) …

Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran In A Forced Aeration System, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

The effect of aeration on the production of xylanase by Trichoderma longibrachiatum on wheat bran in a solid-state fermentation (SSF) system has not been investigated. This study was conducted to investigate the interactive effects of aeration, initial moisture content of the substrate, and depth of the substrate on xylanase activity produced in a tray fermenter. The experiment was conducted as a split plot experiment with factorial treatments and three replications of each treatment combination. The whole plot treatment was aeration rate (0, 2.9, 5.7 L/min/kg bran). Initial moisture content (45, 55, 65% w.b.) and depth of substrate (1.0 and 2.5 …

Arthritis And Rosai-Dorfman Disease Of The Skin: A Diagnostic Dilemma, Irshad Nabi Soomro, Kamran Hameed

Department of Pathology and Laboratory Medicine

No abstract provided.

1999 Scholars And Artists Bibliography, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti

A Study On The Effects Of The N-Terminal Amino Acid Sequence On The Activation Of Human T-Cell Leukemia Virus Type 1 Protease, Hidayah Muhammad Kendall

Chemistry & Biochemistry Theses & Dissertations

Human T-cell leukemia virus type 1 (HTL V-1) is dependent upon the enzymatic activity of its protease for maturation. Maturation of the protease is facilitated by cleavage of specific amino acid residues, followed by dimerization. The effects of the amino acid sequence located N-terminally to the cleavage site on the ability of the protease to become active were the focus of the current study. These amino acid sequences were contributed by the plasmid vector into which the protease gene was inserted.

Surface probability analyses (SPAs) of the vectors, as well as for native sequences which produce the mature protease and …

Cartilage Regeneration On A Large Articular Surface Facilitated By Stress Shielding, Charles L. Mcdowell, Jennifer S. Wayne, Robert Tuten, Hunter H. Mcguire Jr.

Virginia Journal of Science

An animal model for the study of articular cartilage regeneration in-vivo facilitated by stress-shielding is introduced. The object of the model is to test the hypothesis that some form of cartilaginous tissue will grow upon a large joint surface in vivo with the joint in normal motion. The model utilizes the known capability of immature cells to differentiate. The source of cells is bleeding subchondral bone. In addition, the model provides a mechanically shielded environment in which cell differentiation and maturation can occur. The study showed that a substantial amount of tissue will grow in the animal model only when …

P53 And Pcna Expression In Benign, Atypical And Malignant Meningiomas, R Ahmed, I N. Soomro, S A. Aziz, S H. Hasan

Department of Pathology and Laboratory Medicine

Objective: Alterations: p53 genes are turning out to be the most common genetic alterations in human cancers. Due to long half-life of mutated p53, its detection is possible by immunohistochemistry. Proliferating cell nuclear antigen (PCNA) is expressed by dividing cells, hence has been shown to correlate with prognosis. We have used monoclonal antibodies protein DO-7 (p53) and PC10 (PCNA) to see whether their expression correlates with histological grading in meningethelial tumour.
Material and Methods: a Twenty nine meningiomas (20 benign, 7 atypical and 2 malignant) were selected from the records of our laboratory. p53 and PCNA expression was sought by …

Spinal Cord Compression Caused By Metastatic Epithelial Myoepithelial Carcinoma Of The Parotid Gland, I N. Soomro, A S. Hussainy, K Chishti, M H. Pui, S A. Khan, R Ahmed, S H. Hasan

Department of Pathology and Laboratory Medicine

No abstract provided.

Abnormal Electrical Potentials In Lower Limb Muscles After Ankle Sprain Grades I, Ii And Iii, Talal A. Al-Shatti

Rehabilitation Sciences Theses & Dissertations

Fibrillation and positive sharp wave potentials have been found after ankle sprain in muscles around the injured joint. The purpose of this study was to better define and explain the reaction of the muscles around the ankle joint after grades I, II and m ankle sprains. Methods: Fifteen subjects (9 males and 6 females, mean age of 33.2 years) with ankle sprains voluntarily participated in this study. A stress x-ray was done to determine the grade of ankle sprain. Nerve conduction velocity and EMO studies were done three weeks after the injury. None of the patients in this study manifested …

Community-Based Education: Health Promotion For Farmers At The Country Market, M. Susan Jones, Beverly Siegrist

Nursing Faculty Publications

This article describes a unique approach used by baccalaureate nursing students, enrolled in a two-semester community health course, to address the priority of health concerns of agricultural workers in their own rural communities.

Phenotypic Expressions Of Ccr5-Δ32/Δ32 Homozygosity, Giang T. Nguyen, Mary Carrington, Judith A. Beeler, Michael Dean, Louis M. Aledort, Philip M. Blatt, Alan R. Cohen, Donna Dimichele, M. Elaine Eyster, Craig M. Kessler, Barbara Konkle, Cindy Leissinger, Naomi Luban, Stephen J. O'Brien, James J. Goedert, Thomas R. O'Brien

Biology Faculty Articles

Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1.

Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis.

Results: …

Neuronal And Metabolic Increases In Pedunculopontine Nucleus Of Parkinson's Rats, Jonathan Dennis Carlson

Loma Linda University Electronic Theses, Dissertations & Projects

The theoretical mechanisms underlying the symptoms of Parkinson’s disease are based on dysfunctional output from the basal ganglia projected to the cortex via the thalamus. However, the subthalamic nucleus (STN) and substantia nigra pars reticularis (SNr) as part of the basal ganglia, also project to the brainstem, in particular to the pedunculopontine nucleus (PPN). Both the STN and SNr have altered neuronal activity in the 6-hydroxydopamine (6-OHDA) Parkinson’s model rat. Thus, it is hypothesized that the PPN is altered in this model of Parkinson’s disease.

This hypothesis was examined by comparing the rates of glucose metabolism and spontaneous neuronal activity …