Research Methods in Life Sciences Commons^™

Kimberley Research Station Progress Report, 1964 : Insect Pests And Insecticides, K T. Richards

Journal of the Department of Agriculture, Western Australia, Series 4

FOR the past four years, detailed studies have been carried out in regard to the identification, status, bionomics, and control of insects and mite pests attacking the various crops grown in the Ord River Irrigation Area.

Dead Lambs : A Dead Loss, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

During the past year the Animal Health Laboratory has continued its survey into the causes of abortion in sheep and perinatal lamb losses.

This article records the results of this work.

LAMBS and specimens from lambs were submitted to the Animal Health Laboratory from 229 properties situated in 121 areas in this survey during 1964.

Post-mortem examination was carried out on 1,422 lambs and specimens from 61 lambs were also examined.

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

The Effect Of Mulesing And Tailstripping At Lamb Marking On Subsequent Lamb Growth And Incidence Of Fly Strike, R J. Lightfoot, W. L. Mcgarry

Journal of the Department of Agriculture, Western Australia, Series 4

THE modified mules and tailstrip operation has been shown to be highly effective in reducing the incidence of crutch strike in sheep.

In Western Australia the operation is usually performed on ewe weaners or hoggets immediately after either crutching or shearing.

Pig Research Projects For W.A, W J O Wilkie

Journal of the Department of Agriculture, Western Australia, Series 4

The Medina Pig Research Unit now being developed by the Department of Agriculture will be an important addition to pig research facilities in Australia.

The primary function of the unit will be to serve the needs of the pig industry in Western Australia.

In this article, Animal Husbandry Officer W. J. Wilkie, B.V.Sc, discusses some aspects of research to be carried out.

Multi-Timer For Milking Studies, G W R Scott

Journal of the Department of Agriculture, Western Australia, Series 4

Recent research into milking machine operation has proved that the belief of old-time hand-milkers that fast milking was good milking was soundly based.

In current practice the emphasis is on convenient shed and yard design and systematic routine in handling the cows. This permits a high throughput of cows and a high output of milk per labour unit.

The multi-timer designed and built by Mr. G. W. R. Scott and described in this article will prove most valuable for pinpointing where time is lost.

Flock Testing : Weighing, Sampling And Measurement Procedures For Fleece Measurement, H G. Neil, R. J. Lightfoot

Journal of the Department of Agriculture, Western Australia, Series 4

An outline of shearing-shed procedure for the collection of greasy fleece weight records and sampling of the fleece for detailed measurement by the Department of Agriculture Flock Testing Service for Merino stud breeders.

A Study Of Regeneration Of Peripheral Nerve In The Rat, James C. Jay

Loma Linda University Electronic Theses, Dissertations & Projects

Due to improvements in fixation and embedding techniques a restudy of regenerating peripheral nerve was thought advisable in order to better understand and describe the processes which take place.

Femoral nerves of white Wistar rats were used. The lesions were made by either crushing or severing the nerve. After a period of regeneration, varying from 12 hours to six days, the wound was reopened and the injured portion fixed in situ with 1% osmium tetroxide. The tissues were then removed, dehydrated, embedded and sectioned. The sections were stained and studied with a modified RCA EMU2A electron microscope.

Observations revealed that …

Quantitative Evaluation Of Streptococcal Antibodies By A Serum Inhibition Fluorescent Antibody Tube Method, Charles Glenn Nichols

Loma Linda University Electronic Theses, Dissertations & Projects

The development and description of a quantitative method of evaluating streptococcal antibodies by inhibition of fluorescent staining is reported. Suspensions of Streptococcus pyogenes group A, treated with methyl alcohol to reduce auto-fluorescence, were used as antigen. The degree of inhibition of uptake of fluorescent labeled antibody produced by the presence of antibodies in the immune test sera was measured after elution of the labeled antibody. Comparisons were made with controls using normal sera containing no inhibiting antibodies. A method of reporting the results in terms of titer is described.

Streptococcal antibodies were demonstrated quantitatively by this inhibition test in sera …

Lambing Trials At Abydos Research Station, H Suijdendorp

Journal of the Department of Agriculture, Western Australia, Series 4

ABYDOS Research Station is halfway between Port Hedland and Wittenoom Gorge. I t is in country of granitic origin that is largely covered with spinifex (mainly Triodia pungens) and associated species.

The results of a recent lambing trial at Abydos are discussed below:

Field Day : Poultry Research : A Progress Report : Poultry Research Station, Wembley, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

AT the fourth field day held at the Department of Agriculture's Poultry Research Station at Wembley recently, about 150 visitors, most of them commercial poultry farmers, inspected the station and saw the progress of the comprehensive research programme being carried out by officers of the Department's Poultry Branch.

A Comparative Study Of The Growth Of Polyoma Virus In Two Secondary Cell Lines: Mouse Lymphoma And Normal Mouse Fibroblasts, Rheeta M. Stecker

Loma Linda University Electronic Theses, Dissertations & Projects

In comparing the growth of polyoma virus in secondary cell cultures of a mouse lymphoma (P388D₁, a known polyoma-supporting strain) and normal mouse fibroblasts (Earle’s L cells), the following observations have been made.

The L cell is capable of supporting the growth of the polyoma virus with reproduction and release of infectious particles. Viruses appear in the fluid from time to time over a period of several weeks. This probably represents a carrier state of the virus in L cells. Many of the L cells in infected cultures are not killed by the virus infection.

Virus particles released …

Time Of Lambing Experiment : Merredin Research Station, 1959-60 (Progress Report), W L. Mcgarry, I. K. Stott

Journal of the Department of Agriculture, Western Australia, Series 4

FOLLOWING upon the Time of Lambing Experiments at Esperance Plains Research Station (Journal of Agriculture, May, 1960) it was decided to carry out similar work with Merino sheep under the relatively harsher environment at Merredin Research Station.

Identical Twins, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

THE response to the Department's appeal for identical twin calves has been gratifying, and so far this year five sets have been located and collected.

In view of the rare occurrence of identical twinning, this indicates excellent co-operation from dairy farmers.

Poisoning Rabbits From The Air, C D. Gooding

Journal of the Department of Agriculture, Western Australia, Series 4

AERIAL baiting gives spectacular kills of rabbits in country which cannot be effectively treated by conventional means, and has been widely adopted in New Zealand and other States in Australia.

Here Mr. Gooding reports on developments in aerial baiting in Victoria, and suggests practical applications in Western Australia.

Identicial Twins : A Valuable Aid To Animal Husbandry Research, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

A T a recent field day at Wokalup Research Station a set of identical twins was shown as being the start of a project to build up a herd or number of such, for research and extension work.

The Pacemaker Function Of The Small Intestine, Robert W. Hasselbrack

Loma Linda University Electronic Theses, Dissertations & Projects

New evidence is presented to clarify to some extent the significance of the gradient of rhythmicity in the small intestine of the unanesthetized dog. The gradient of rhythmicity was studied on five dogs prepared with duodenal fistulas. Rhythmic contractions were recorded from the small intestine by a small rubber balloon. The gradient of rhythmicity was found to decrease in a stepwise manner as the distance from the pylorus is increased. Roughly, the rhythmic contractions were found to be 18 to 19 per minute in the duodenum and 14 to 15 per minute in the ileum. In the terminal ileum there …

Better Rations For Broiler Production, P Smetana

Journal of the Department of Agriculture, Western Australia, Series 4

DURING 1959, trials were conducted at the Poultry Research Station, Herdsman Lake, with the object of developing a broiler-feeding ration that was better than the one at present recommended by the Department of Agriculture and commonly used by local broiler producers.

Free Feeding For The Control Of Rabbits, C Marshall

Journal of the Department of Agriculture, Western Australia, Series 4

IN common with most other farm work, the control of rabbits calls for a high degree of skill, patience and perseverance.

There is no short cut to success, and if results are required we must be prepared to give of our best.

Failures of control works are experienced at various times, and the reasons can generally be traced back to something we have failed to do or some short cut we have tried to take.

However, we should not lose heart from one failure. During normal farming procedure, if a farmer's crop fails he does not give up planting in …

The Success Of The Rabbit 'Killer' Trials, A R. Tomlinson

Journal of the Department of Agriculture, Western Australia, Series 4

SUCCESS well beyond what was anticipated has been achieved in a two year trial of a modified version of the rabbit "killer" method.

In the "killer" method, trained officers are employed to kill rabbits instead of to inspect and supervise the activities of farmers and, in fact, these officers do all the actual work of destruction.

This system was advocated by the W.A. Royal Commission which reported on Vermin Control in this State in 1944, and it was used successfully in New Zealand.

These trials were applied only to poisoning and not to overall destruction measures. The main object was …

Galah Poisoning : Kill The "Cocky", John L. Long, F. Vagg

Journal of the Department of Agriculture, Western Australia, Series 4

NO! Not the one that tills the land, but his enemy, the pink and grey galah (Kakatoe rosei-capilla).

Farmers have usually found the destruction of galahs damaging standing crops and bagged, or stored grain, fairly difficult.

Past methods of boiling grain and adding sweetened substances are rather laborious and require a lot of time.

Now, a quick and relatively simple method is available to farmers, using paraffin oil to adhere poison to the grain.

Vermin Control In W.A, A R. Tomlinson

Journal of the Department of Agriculture, Western Australia, Series 4

THE annual report of the Agriculture Protection Board of W.A. for 1958-1959 indicated that while vermin control had continued at a relatively high standard throughout the State, most of the animal and bird pests were at the lowest level within the memory of most people.

However, there were ample warnings that any relaxation in control work could have rapid and serious consequences.

Pharmacological Studies Of Antidiabetic Chinese Herbs, Mun Fa Lee

Loma Linda University Electronic Theses, Dissertations & Projects

Rehmannia glutinosa, Atractylus ovata, and Polygonatum chinense have all been employed by the Chinese as oral antidiabetic agents and reported by Min (1928), Kin (1931), and Li et al. (1936) to possess hypoglycemic activity. This prompted the pharmacological studies of these herbs.

Materials and methods. Their pharmacognosy was condensed from all available Chinese and scientific literature. Preliminary chemical and physical analyses included determinations for moisture, ash, crude fat, carbohydrates, protein, phosphorus, pH, alkaloids, melting point, and spectroscopy. Employing the Somogyi-Nelson method, their glycemic activity was studied in rats, rabbits, and dogs. Stress reactions were minimized. The effect …

The Effect Of Preweanling Administration Of Carbohydrates Used In Infant Formulas On Subsequent Dental Caries In White Rats, Erlinda Amor Romulo

Loma Linda University Electronic Theses, Dissertations & Projects

The high incidence of tooth decay, one of the most common diseases found in all age groups, gives a reason for the study of methods to prevent dental caries. Certain dietary factors, specifically carbohydrates, have been implicated in the decay process. Since diet influences both the oral and systemic environments in which the teeth develop, mature, age, and decay (1), many studies have been conducted to determine the effects of oral administration of different carbohydrates on dental caries. The extrinsic effects of diet on the mature erupted tooth have, however, been the major concern of the research (2).

The first …

The Effect Of Adrenal Steroid Hormones On The Protein Content Of Tumorous Livers, Duncan W. Martin

Biology ETDs

Protein is basic to the structure and function of protoplasm. Enzyme systems which regulate the rate of cellular reactions are primarily composed of protein and many of these enzymatic systems influence the metabolism of less specialized proteins that form the major structural material of protoplasm. It is believed that experimental procedures which modify protein composition of cells may also influence the activity of intra-cellular enzymes. In experimental live cancer, it has been shown that the activities of a number of oxidative enzymes are modified as the carcinogenic process proceeds (Richmond, 1955, p.38). Furthermore, it has been demonstrated that endocrine function …

A Study Of The Renal Vascular Supply In The Domestic Cat, Elizabeth Rosen

Bachelors’ Theses

Studies on the normal and abnormal vascularization of the renal vessels in the domestic cat (felis domestica) indicate the frequency of anomalies that occur, but in most of the studies it is the inferior vena cava that is most often recorded. Among the occasional references to several renal veins or arteries we have Huntington and McClure, Elmer Butler, Gaylord Bates, William Darrach, and C.H. Fortune. Yet no study seems to have been made of individuals of any particular class. A superficial observation seems to indicate a large number of cats used in the laboratory had multiple renal veins. Hence a …

Histological Study Of The Small Intestine Of The Common Green Snake, Thamnophis Sirtalis, Robert George Stineman

Bachelors’ Theses

Although much work has been done histologically with the vertebrates, very little seems to have been done on the intestinal tract of the reptile. This is especially true of the snakes, despite the abundant sources of material. Reptiles are one of the first classes of vertebrates in which there is a distinction made between small and large intestine. Histology textbooks devote only a few lines to the histology of the reptilian small intestine. For this reason it seems that it would be worthwhile to make a detailed study of that portion of the intestinal tract immediately distal to the duodenum …

The Effect Of Adrenaline Injection On Glycogenolysis And Glycogenesis In Rat Livers, Virginia Mary Zingsheim

Bachelors’ Theses

Present day knowledge concerning the liver is derived from many sources and the accumulation of many methods of investigation. Sugar, which is needed by the body for energy, is stored in the liver as glycogen. Claude Bernard (26), an eminent physiologist, was the first to suspect that carbohydrates were stored in the liver. Faulty glycogen synthesis and hydrolysis results in one of our most widespread diseases, diabetes. Thus, many biochemists and physiologists have tried to find the mechanism of the process, and the primary and secondary facts which affect glycogenesis and glycogenolysis. Although the chemical secretions and hormones of such …

Staining Techniques In The Study Of The Blood, Donald E. Winnik

Bachelors’ Theses

Since the invention of the microscope, the study of the blood and its constituents has been a major interests of histologists and hematologists. The vast amount of material written about the subject could not have been accomplished without the development and use of staining techniques which made possible a more precise observation of the nature of the blood. At the present time there are many procedures and stains which vary greatly in their application and use. It is the purpose of this thesis to review these many methods and catalogue them according to their application in the blood techniques.

The Spinal Nerves And Spinal Cord Of The Frog, Laverne A. Penn

Bachelors’ Theses

The common grass frog, Rana pipiens as wall as the bull frog, Rana catesbiana have served during many decades as material from which both the student of zoology and the investigator in biological problems have gathered valuable information about the anatomy and physiology of the common systems. Among these systems the central, peripheral, and sympathetic nervous system plays a coordinating role, because of the ramifying nature of its organization. It is the purpose of this thesis to investigate the gross and histological structure of these branches known as spinal nerves. When one inspects the literature on the nervous system of …