Plant Breeding and Genetics Commons^™

2023 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, H. Dhammu

Bulletins 4000 -

Welcome to the 2023 edition of the Crop Sowing Guide for WA, which introduces 31 new variety releases: two wheat, seven barley (one as feed only and six currently under malt evaluation), 15 canola, three hay-only oat and four lentil varieties.

The Crop Sowing Guide for WA has been compiled by officers in the Department of Primary Industries and Regional Development. It provides information to support variety decisions for each of the major crops for the upcoming season.

In this edition, the canola NVT series are now identified by GRDC/NVT as ‘Low-Med Rainfall’ and ‘Med–High Rainfall’ to reflect the environments …

Variation In Morpho‑Physiological And Metabolic Responses To Low Nitrogen Stress Across The Sorghum Association Panel, Marcin Grzybowski, Mackenzie Zwiener, Mackenzie Zwiener, Hongyu Jin, Nuwan K. Wijewardane, Abbas Atefi, Michael J. Naldrett, Sophie Alvarez, Yufeng Ge, James C. Schnable

Center for Plant Science Innovation: Faculty Publications

Background: Access to biologically available nitrogen is a key constraint on plant growth in both natural and agricultural settings. Variation in tolerance to nitrogen deficit stress and productivity in nitrogen limited conditions exists both within and between plant species. However, our understanding of changes in different phenotypes under long term low nitrogen stress and their impact on important agronomic traits, such as yield, is still limited.

Results: Here we quantified variation in the metabolic, physiological, and morphological responses of a sorghum association panel assembled to represent global genetic diversity to long term, nitrogen deficit stress and the relationship …

Analysis Effect Of Shade Level On The Physiological And Anatomical Characteristics Of Hybrid Phalaenopsis Orchid At The Acclimatization Stage, Ade Arisma Fauziah, Nintya Setiari, Endang Saptiningsih

Jurnal Kultivasi

Production of hybrid Phalaenopsis seedlings is generally applied by in vitro culture techniques. The final stage of in vitro culture is acclimatization. The acclimatization stage is crucial because the plantlets must adapt to the ex-vitro environment. Shade supports plantlet growth during the early stages of acclimatization. This study aims to determine the effect of shade level on the physiological and anatomical characteristics of the Phalaenopsis hybrid orchid at the acclimatization stage. The research used Phalaenopsis plantlet hybrid and shading level. Plantlets were shaded at 40%, 55%, and 70%. Parameters measured included: photosynthetic pigment content, number and area of leaves, number …

Effect Of Paclobutrazol On Growth And Root Morphology Of 12 Crossed Stevia In Vitro, Suseno Amien, Qurrota Aini, Noladhi Wicaksana

Jurnal Kultivasi

Stevia (Stevia rebaudiana Bertoni) is a low-calorie alternative sweetener. The superior varieties of Stevia in Indonesia are limited. Availability of seeds plays an important role in the Stevia plant breeding program for both the selection stage and the production of high-yielding varieties. Tissue culture is the best method of Stevia propagation, but the low adaptability of plantlets causes death in the acclimatization phase. The growth and root morphology of 12 crossbreed Stevia were evaluated. A Completely Randomized Design was used with factorial consisting of two factors were Stevia genotypes (STG1,7,8,10, SBG3,4,5,7,10, SGB2,3, and SBT11) and the plant growth regulator (PGR) …

Genetic And Biochemical Investigation Of Seed Fatty Acid Accumulation In Arabidopsis, Chinedu Charles Nwafor, Delin Li, Ping Qin, Long Li, Wei Zhang, Yuanwei Zhou, Jingjing Xu, Yongtai Yin, Jianbo Cao, Limin He, Fu Xiang, Chao Liu, Liang Guo, Yongming Zhou, Edgar B. Cahoon, Chunyu Zhang

Center for Plant Science Innovation: Faculty Publications

As a vegetable oil, consisting principally of triacylglycerols, is the major storage form of photosynthetically-fixed carbon in oilseeds which are of significant agricultural and industrial value. Photosynthesis in chlorophyll-containing green seeds, along with photosynthesis in leaves and other green organs, generates ATP and reductant (NADPH and NADH) needed for seed fatty acid production. However, contribution of seed photosynthesis to fatty acid accumulation in seeds have not been well-defined. Here, we report the contribution of seed-photosynthesis to fatty acid production by probing segregating green (photosynthetically-competent) and non-green or yellow (photosynthetically-non-competent) seeds in siliques of an Arabidopsis chlorophyll synthase mutant. Using this …

Insect Pest Management With Sex Pheromone Precursors From Engineered Oilseed Plants, Hong-Lei Wang, Bao-Jian Ding, Jian-Qing Dai, Tara J. Nazarenus, Rafael Borges, Agenor Mafra-Neto, Edgar B. Cahoon, Per Hofvander, Sten Stymne, Christer Löfstedt

Center for Plant Science Innovation: Faculty Publications

Pheromones have become an environmentally friendly alternative to conventional insecticides for pest control. Most current pheromone-based pest control products target lepidopteran pests of high-value crops, as today’s manufacturing processes cannot yet produce pheromones at low enough costs to enable their use for lower-value crops, especially commodity crops. Camelina sativa seeds genetically modified to express (Z)-11-hexadecenoic acid, a sex pheromone precursor of several moth species, provided the oil from which the precursor was isolated, purified and transformed into the final pheromone. Trap lures containing this pheromone were then assessed for their capacity to manage moth pests in the field. Plant-derived pheromone …

The Pho1;2a'-M1.1 Allele Of Phosphate1 Conditions Misregulation Of The Phosphorus Starvation Response In Maize (Zea Mays Ssp. Mays L.), Ana Laura Alonso-Nieves, M. Nancy Salazar-Vidal, J. Vladimir Torres-Rodríguez, Leonardo M. Pérez-Vázquez, Julio A. Massange-Sánchez, C. Stewart Gillmor, Ruairidh J. H. Sawers

Center for Plant Science Innovation: Faculty Publications

Plant PHO1 proteins play a central role in the translocation and sensing of inorganic phosphate. The maize (Zea mays ssp. mays) genome encodes two co-orthologs of the Arabidopsis PHO1 gene, designated ZmPho1;2a and ZmPho1;2b. Here, we report the characterization of the transposon footprint allele Zmpho1;2a'-m1.1, which we refer to hereafter as pho1;2a. The pho1;2a allele is a stable derivative formed by excision of an Activator transposable element from the ZmPho1;2a gene. The pho1;2a allele contains an 8-bp insertion at the point of transposon excision that disrupts the reading frame and is predicted to …

Divergent Evolution Of Extreme Production Of Variant Plant Monounsaturated Fatty Acids, Lu Gan, Kiyoul Park, Jin Chai, Evan M. Updike, Hyojin Kim, Adam Voshall, Sairam Behera, Xiao-Hong Yu, Yuanheng Cai, Chunyu Zhang, Mark A. Wilson, Jeffrey P. Mower, Etsuko Moriyama, Chi Zhang, Sireewan Kaewsuwan, Qun Liu, John Shanklin, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Metabolic extremes provide opportunities to understand enzymatic and metabolic plasticity and biotechnological tools for novel biomaterial production. We discovered that seed oils of many Thunbergia species contain up to 92% of the unusual monounsaturated petroselinic acid (18:1Δ6), one of the highest reported levels for a single fatty acid in plants. Supporting the biosynthetic origin of petroselinic acid, we identified a Δ6-stearoyl-acyl carrier protein (18:0-ACP) desaturase from Thunbergia laurifolia, closely related to a previously identified Δ6-palmitoyl-ACP desaturase that produces sapienic acid (16:1Δ6)- rich oils in Thunbergia alata seeds. Guided by a T. laurifolia desaturase crystal structure obtained in this study, …

Association Mapping Across A Multitude Of Traits Collected In Diverse Environments In Maize, Ravi V. Mural, Guangchao Sun, Marcin Grzybowski, Michael C. Tross, Hongyu Jin, Christine Smith, Linsey Newton, Carson M. Andorf, Margaret R. Woodhouse, Addie M. Thompson, Brandi Sigmon, James C. Schnable

Center for Plant Science Innovation: Faculty Publications

Classical genetic studies have identified many cases of pleiotropy where mutations in individual genes alter many different phenotypes. Quantitative genetic studies of natural genetic variants frequently examine one or a few traits, limiting their potential to identify pleiotropic effects of natural genetic variants. Widely adopted community association panels have been employed by plant genetics communities to study the genetic basis of naturally occurring phenotypic variation in a wide range of traits. High-density genetic marker data—18M markers—from 2 partially overlapping maize association panels comprising 1,014 unique genotypes grown in field trials across at least 7 US states and scored for 162 …

Genetically Explicit Model May Explain Multigenerational Control Of Emergent Turing Patterns In Hybrid Mimulus, Emily Simmons

Biology and Medicine Through Mathematics Conference

No abstract provided.

The Potential Role Of Phenotypic Plasticity In The Ability Of Hydrocotyle Bonariensis To Occupy Two Different Habitats, Harold Ralph Parsons Iii

Honors College Theses

Phenotypic plasticity is the ability of an organism to change morphology and/or physiology in response to changes in the environment. Hydrocotyle bonariensis is a coastal perennial herb found in both coastal sand dunes and inland coastal plain habitats in Georgia. The purpose of this study was to determine if there were differences in leaf morphology for populations of H. bonariensis in coastal sand dune and inland coastal plain habitats. Leaf morphology and microenvironmental variables were compared between H. bonariensis populations at Tybee Island (TI), GA, representing the coastal sand dune habitat, and Georgia Southern University Armstrong Campus (GSU) in Savannah, …

Identification And Characterization Of Circular Rnas In Brassica Rapa In Response To Plasmodiophora Brassicae, Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao

Center for Plant Science Innovation: Faculty Publications

Plasmodiophora brassicae is a soil-borne pathogen that attacks the roots of cruciferous plants and causes clubroot disease. CircRNAs are noncoding RNAs, widely existing in plant and animal species. Although knowledge of circRNAs has been updated continuously and rapidly, information about circRNAs in the regulation of clubroot disease resistance is extremely limited in Brassica rapa. Here, Chinese cabbage (BJN 222) containing clubroot resistance genes (CRa) against P. brassicae Pb4 was susceptible to PbE. To investigate the mechanism of cicRNAs responsible for clubroot disease resistance in B. rapa, circRNA-seq was performed with roots of ‘BJN 222’ at 0, 8, and 23 days …

Perbandingan Daya Hasil Dan Toleransi Naungan Berbagai Genotipe Jagung Padjadjaran Pada Naungan Eukaliptus, Adilah Nurul Fitrah, Nono Carsono, Dedi Ruswandi

Jurnal Kultivasi

Kebutuhan jagung sebagai bahan pangan, pakan dan industri selalu meningkat, namun terdapat kendala dalam produksi jagung domestik, yaitu konversi lahan pertanian. Sistem agroforestri eukaliptus-jagung dapat menjadi alternatif yang digunakan untuk pengembangan jagung. Tujuan penelitian ini untuk mengidentifikasi daya hasil, mengestimasi nilai indeks toleransi, dan menyeleksi galur jagung Padjadjaran yang toleran terhadap naungan Eucalyptus sp. Penelitian ini dilaksanakan pada April – November 2019 di Sanggar Penelitian Latihan dan Pengembangan Pertanian (SPLPP) Fakultas Pertanian Universitas Padjadjaran, Bandung. Rancangan percobaan menggunakan rancangan petak terbagi (Split plot) dengan dua faktor (naungan dan genotipe) dan tiga ulangan. Petak utama adalah faktor naungan yang terdiri dari …

Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …

Fddm1 And Fddm2, Two Sgs3-Like Proteins, Function As A Complex To Affect Dna Methylation In Arabidopsis, Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R. Alfano, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

DNA methylation is an important epigenetic modification required for the specific regulation of gene expression and the maintenance of genome stability in plants and animals. However, the mechanism of DNA demethylation remains largely unknown. Here, we show that two SGS3-like proteins, FACTOR OF DNA DEMETHYLATION 1 (FDDM1) and FDDM2, negatively affect the DNA methylation levels at ROS1-dependend DNA loci in Arabidopsis. FDDM1 binds dsRNAs with 50 overhangs through its XS (rice gene X and SGS3) domain and forms a heterodimer with FDDM2 through its XH (rice gene X Homology) domain. A lack of FDDM1 or FDDM2 increased DNA methylation levels …

Qteller: A Tool For Comparative Multi-Genomic Gene Expression Analysis, Margaret R. Woodhouse, Shatabdi Sen, David Schott, John L. Portwood Ii, Michael Freeling, Justin W. Walley, Carson M. Andorf, James Schnable

Center for Plant Science Innovation: Faculty Publications

Motivation: Over the last decade, RNA-Seq whole-genome sequencing has become a widely used method for measuring and understanding transcriptome-level changes in gene expression. Since RNA-Seq is relatively inexpensive, it can be used on multiple genomes to evaluate gene expression across many different conditions, tissues and cell types. Although many tools exist to map and compare RNA-Seq at the genomics level, few web-based tools are dedicated to making data generated for individual genomic analysis accessible and reusable at a gene-level scale for comparative analysis between genes, across different genomes and meta-analyses. Results: To address this challenge, we revamped the comparative gene …

Getting To The Root Cause: The Genetic Underpinnings Of Root System Architecture And Rhizodeposition In Sorghum, Farren Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Plants are some of the most diverse organisms on earth, consisting of more than 350,000 different species. To understand the underlying processes that contributed to plant diversification, it is fundamental to identify the genetic and genomic components that facilitated various adaptations over evolutionary history. Most studies to date have focused on the underlying controls of above-ground traits such as grain and vegetation; however, little is known about the “hidden half” of plants. Root systems comprise half of the total plant structure and provide vital functions such as anchorage, resource acquisition, and storage of energy reserves. The execution of these key …

Periode Inkubasi, Tingkat Keparahan, Dan Ketahanan Sepuluh Genotipe Padi Harapan Terhadap Penyakit Hawar Daun Bakteri Strain Iii, Iv, Dan Viii, Nono Carsono, Anggita Dewi, Noladhi Wicaksana, Santika Sari

Jurnal Kultivasi

Genotipe padi harapan dari UNPAD memiliki keunggulan pada daya hasil, tahan terhadap wereng coklat, memiliki amilosa sedang, aromatik dan kualitas bulir yang sangat baik. Pengujian ketahanan genotipe padi harapan yang berasal dari hasil seleksi molekuler dan fenotipik terhadap penyakit hawar daun bakteri (HDB) sangatlah dibutuhkan, karena ketahanan terhadap penyakit ini merupakan salah satu syarat pelepasan varietas padi di Indonesia. Penyakit HDB disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae (Xoo) merupakan penyakit penting yang sering menyerang tanaman padi. Tujuan dari penelitian ini adalah untuk memperoleh informasi potensi genotipe-genotipe padi yang tahan terhadap penyakit HDB yang disebabkan oleh Xoo strain III, IV, …

Crispr Gene Editing Drivers, Barriers And Prospects: A Comparative Study Among Plant Scientists Globally, Adriaan Johannes De Lange

Graduate Theses and Dissertations

The introduction of CRISPR gene editing into food crops has potential to contribute to food security and sustainable food production globally. To date, most scientific studies have focused on consumer perception of CRISPR gene edited foods or the potential benefits and risks of the CRISPR technology and none have focused on the perceptions of plant scientists concerning CRISPR gene editing. This study aimed to explore the investments, functions, barriers, benefits for specific crops and beneficiaries of CRISPR gene editing according to plant scientists, by distributing an online survey in which 1,040 plant scientists active across six continents and in both …

Hyperspectral Reflectance-Based Phenotyping For Quantitative Genetics In Crops: Progress And Challenges, Marcin Grzybowski, Kuwan K. Wijewardane, Abbas Atefi, Yufeng Ge, James C. Schnable

Center for Plant Science Innovation: Faculty Publications

Many biochemical and physiological properties of plants that are of interest to breeders and geneticists have extremely low throughput and/or can only be measured destructively. This has limited the use of information on natural variation in nutrient and metabolite abundance, as well as photosynthetic capacity in quantitative genetic contexts where it is necessary to collect data from hundreds or thousands of plants. A number of recent studies have demonstrated the potential to estimate many of these traits from hyperspectral reflectance data, primarily in ecophysiological contexts. Here, we summarize recent advances in the use of hyperspectral reflectance data for plant phenotyping, …

2022 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, S. Power

Bulletins 4000 -

Welcome to the 2022 edition of the Crop Sowing Guide for WA, which introduces 27 new variety releases: three wheat, nine barley (three recently accredited for malt and six currently under malt evaluation), twelve canola, two field pea and a lentil.

The Crop Sowing Guide for WA has been compiled by officers in the Department of Primary Industries and Regional Development. It provides information to support variety decisions for each of the major crops for the upcoming season.

In this edition, lupin agzones have been reduced from 8 agzones to 6 so that all varieties for each of the major …

Mutations In Several Auxin Biosynthesis Genes And Their Effects On Plant Phenotypes In Arabidopsis, Gabriela Hernandez, Lauren Huebner, Bethany Karlin Zolman

Undergraduate Research Symposium

Auxins are important hormones in plants that regulate growth and development. Disruptions in the auxin biosynthesis pathway result in morphological changes in phenotypes in the model plant Arabidopsis thaliana, including differences in root and leaf formation. Mutations in the Tryptophan Aminotransferase of Arabidopsis (TAA1) and YUCCA (YUC4) genes interfere with the plant's ability to synthesize Indole-3-acetic acid (IAA), the primary auxin involved in plant development. IBR1 and IBR3 act in the multistep conversion of indole-3-butyric acid (IBA) to IAA. ILL2, IAR3, and ILR1 hydrolyze IAA-amino acid conjugates into free IAA. The goal of …

Genetic Diversity And Population Structure Of Sugarcane (Saccharum Officinarum L.) Collected From Seven Philippine Regions For Use As Breeding Stocks For Crop Improvement, Jan Leandro Acedera, Jhun Laurence Rasco, Jemimah Banganan, Antonio Lalusin

The Philippine Agricultural Scientist

Selection of parents for hybridization relies on the information on their genetic relationship and diversity which are essential in any breeding program. This study aimed to estimate the extent of genetic diversity and population structure of 76 sugarcane accessions from seven regions in the Philippines using 57 morphological characters and 50 microsatellite markers. The sugarcane collections exhibited moderate to high diversity with mean of H’ = 0.72 for qualitative and H’ = 0.75 for quantitative morphological characters, respectively. This is corroborated by the analysis of variance (ANOVA) of agronomic parameters, except for stalk length. Unweighted Pair Group Method with Arithmetic …

Three Lc-Ms Plant Metabolomics Studies Of Hop (Humulus) Species: Wild H. Neomexicanus, Drought Stress, And Agricultural Terroir, Taylan Morcol

Dissertations, Theses, and Capstone Projects

The hop plant (Humulus L., Cannabaceae) is a dioecious, perennial, twining vine with a long history of human use. Nowadays, hop plants are generally grown for their inflorescences (“cones”), which are used in brewing for their phytochemical metabolites. Many of these metabolites are involved in plant stress response and communication. Genetics and environment are two major factors that affect plant metabolism. In three separate metabolomics studies, this project examined the effects of both genetic and environmental factors on hop phytochemistry.

In the first study, 23 hop genotypes were grown in two different locations in the Pacific Northwest region of …

Primary Industries Development Research Highlights 2021, Department Of Primary Industries And Regional Development, Tim Scanlon

Books & book chapters

The Primary Industries Development Research Highlights 2021 showcases the breadth and depth of the Department of Primary Industries and Regional Development’s research and development activities over the past several years.

Stories featured in Research Highlights 2021 stem from about 60 (of 140) current and recently-completed projects undertaken by the Department of Primary Industries and Regional Development’s (DPIRD) 1100 scientists, technical experts and economists throughout the State.

Explore our Research Highlights 2021.

The publication demonstrates the innovative and applicable research that DPIRD and its collaborators and investment partners deliver to Western Australia.

Download the Research Highlights 2021 here. Alternatively, …

Marker-Assisted Selection To Determine The Introgression Of Rpv-3 Mediated Downy Mildew Resistance In 'Chambourcin' X 'Caberenet Sauvignon' Grapevine Population, Kavya Sri Tummala

Graduate Theses/Dissertations

Downy mildew caused by the fungus, Plasmopara viticola in the grapes is one of the major diseases affecting crop yield throughout the world. Breeding grapes via conventional methods for the downy mildew resistance will take approximately 25-30 years to fully evaluate and release new cultivars. Because of the long breeding cycle in woody plant species like grape, developing DNA markers linked to genes or quantitative trait loci (QTLs) for horticultural traits can greatly accelerate the breeding process and allow a much more accurate selection of progeny. This study aims to determine the presence of resistance in the 267 F1 genotypes …

Understanding Potassium Toxicity Stress Responses Of The Extremophyte Schrenkiella Parvula Using Systems Biology Approaches, Pramod Pantha

LSU Doctoral Dissertations

Schrenkiella parvula is an extremophyte model closely related to Arabidopsis thaliana and Brassica crops. Its natural habitat includes shores of saline lakes in the Irano-Turanian region. It has adapted to grow in soils rich in multiple salts including Na⁺ and K⁺. I have investigated the genetic basis for high K⁺ tolerance in plants using S. parvula as a stress tolerant model compared to the premier plant model, Arabidopsis thaliana which is highly sensitive to salt stresses using physiological, ionomic, transcriptomic, and metabolomic approaches. Under high K⁺ stress, root system architecture changes significantly compared to control …

Summaries Of Arkansas Cotton Research 2020, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

In the five years before 2020, cotton acreage in Arkansas had steadily increased from an all-time low of 210,000 acres in 2015 to 610,000 planted acres in 2019. One reason for the increase can be attributed to a downturn in prices received by producers for commodities such as corn and soybean, which compete for acres with cotton. With the worldwide COVID-19 pandemic, cotton mill use dropped significantly during the first half of 2020 https://www.cottongrower.com/market-analysis/ncc-cotton-demand-returning-as-u-s-and-world-economies-rebound/. This disruption of the cotton supply chain was felt across the entire cotton industry. The resulting downturn of cotton prices prior to planting impacted producers planting …

Toward Sustainable Production Of Value-Added Bioenergy And Industrial Oils In Oilseed And Biomass Feedstocks, Kiyoul Park, Sanju A. Sanjaya, Truyen Quach, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Plant fatty acids are used forfood, feed, fuel, and industrial materials. Structurally and chemically diverse fatty acids, referred to as unusual or specialized fatty acids, are found in the seed oils of diverse plant species. Many unusual fatty acids have potential use as alternative and renewable sources of biofuels and biobased industrial feedstocks due to their variant structures' physical or functional properties. Oils enriched in these fatty acids can increase the value of oilseed crops and provide co-products that can be readily extracted from lignocellulosic materials in biomass crops. Here, we describe recent progress in strategies for enhancement of oil …

Disruption Of Long-Chain Base Hydroxylation Alters Growth And Impacts Sphingolipid Synthesis In Physcomitrella Patens, Abraham R. Steinberger, William Oscar Merino, Rebecca E. Cahoon, Edgar B. Cahoon, Daniel V. Lynch

Center for Plant Science Innovation: Faculty Publications

Sphingolipids have roles as membrane structural components and as bioactive molecules in plants. In Physcomitrella patens, 4-hydroxysphinganine (phytosphingosine, t18:0) is the predominant sphingolipid long-chain base (LCB). To assess the functional significance of t18:0, CRISPR-Cas9 mutagenesis was used to generate mutant lines lacking the sole SPHINGOID BASE HYDROXYLASE (SBH) gene encoding the hydroxylase responsible for converting sphinganine (d18:0) to t18:0. Total sphingolipid content in sbh protonemata was 2.4-fold higher than in wild-type. Modest changes in glycosyl inositolphosphorylceramide (GIPC) glycosylation patterns occurred. Sphingolipidomic analyses of mutants lacking t18:0 indicated modest alterations in acyl-chain pairing with d18:0 in GIPCs and ceramides, but dramatic …