Plant Biology Commons^™

Transcriptional And Post-Translational Regulation Of Terpenoid Indole Alkaloid Biosynthesis In Catharanthus Roseus, Priyanka Paul

Theses and Dissertations--Plant and Soil Sciences

Catharanthus roseus (Madagascar periwinkle) is the exclusive source of an array of terpenoid indole alkaloids (TIAs) that are used in the treatments of hypertension and certain types of cancer. TIA biosynthesis is under stringent spatiotemporal control and is induced by jasmonate (JA) and fungal elicitors. Tryptamine, derived from the indole branch, and secologanin from the iridoid branch are condensed to form the first TIA, strictosidine. Biosynthesis of TIA is regulated at the transcriptional level and several transcription factors (TFs) regulating the expression of genes encoding key enzymes in the pathway have been isolated and characterized. The JA-responsive APETALA2/ETHYLENE RESPONSE FACTOR …

Invasion Dynamics Of The Exotic Liana Euonymus Fortunei (Turcz.) Hand.-Mazz. (Wintercreeper), Todd J. Rounsaville

Theses and Dissertations--Biology

Elevated atmospheric CO₂ has been implicated as a driver of increased liana abundance worldwide. Known as disturbance creators and beneficiaries, lianas possess the potential to significantly influence forest ecosystems. I investigated the early-invasion dynamics of Euonymus fortunei (wintercreeper), an evergreen liana that is invading forests in eastern North America, disrupting native plant communities and ecosystem functions.

Wintercreeper is widely cultivated as an ornamental groundcover, frequently invading natural areas via asexual stem growth. Invasion of remote natural areas is dependent upon seed transport and may occur less frequently. I examined the mechanisms of seed dormancy by conducting a ‘move-along’ experiment …

Increasing Renewable Oil Content And Utility, William Richard Serson

Theses and Dissertations--Plant and Soil Sciences

Since the dawn of agriculture man has been genetically modifying crop plants to increase yield, quality and utility. In addition to selective breeding and hybridization we can utilize mutant populations and biotechnology to have greater control over crop plant modification than ever before. Increasing the production of plant oils such as soybean oil as a renewable resource for food and fuel is valuable. Successful breeding for higher oil levels in soybean, however, usually results in reduced protein, a second valuable seed component. We show that by manipulating a highly active acyl-CoA: diacylglycerol acyltransferase (DGAT) the hydrocarbon flux to oil in …

Expression Of A Constitutively Active Nitrate Reductase Variant In Tobacco Reduces Tobacco-Specific Nitrosamine Accumulation In Cured Leaves And Cigarette Smoke, Jianli Lu, Leichen Zhang, Ramsey S. Lewis, Lucien Bovet, Simon Goepfert, Anne M. Jack, James D. Crutchfield, Huihua Ji, Ralph E. Dewey

Kentucky Tobacco Research and Development Center Faculty Publications

Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N-nitrosonornicotine (NNN), have been shown to be strong carcinogens in numerous animal studies. We investigated the potential of molecular genetic strategies to lower nitrate levels in burley tobaccos by overexpressing genes encoding key enzymes of the nitrogen-assimilation pathway. Of the various constructs tested, only the expression of a constitutively active nitrate reductase (NR) …

Changes In Oxidative Patterns During Dormancy Break By Warm And Cold Stratification In Seeds Of An Edible Fruit Tree, Dilinuer Shalimu, Jia Sun, Carol C. Baskin, Jerry M. Baskin, Liwei Sun, Yujun Liu

Biology Faculty Publications

The transition from seed dormancy to germination is triggered by environmental factors, and in pomegranate (Punica granatum) seeds higher germination percentages are achieved by warm + cold stratification rather than by cold stratification alone. Our objective was to define the pattern of internal oxidative changes in pomegranate seeds as dormancy was being broken by warm + cold stratification and by cold stratification alone. Embryos isolated from seeds after 1–42 days of warm stratification, after 56 days of warm stratification + 7, 28 or 56 days of cold stratification, and after 1–84 days of cold stratification alone, were used …

Insights Into Triterpene Metabolism In Model Monocotyledonous And Oilseed Plants Genetically Engineered With Genes From Botryococcus Braunii, Chase F. Kempinski

Theses and Dissertations--Plant and Soil Sciences

Isoprenoids are one of the most diverse classes of natural products and are all derived from universal five carbon, prenyl precursors. Squalene and botryococcene are linear, hydrocarbon triterpenes (thirty carbon compounds with six prenyl units) that have industrial and medicinal values. Squalene is produced by all eukaryotes as it is the first committed precursor to sterols, while botryococcene is uniquely produced by the green algae, Botryococcus braunii (race B). Natural sources for these compounds exist, but there is a desire for more renewable production platforms. The model plant Arabidopsis thaliana was engineered to accumulate botryococcene and squalene in its oil …

Investigation Into The Cell Wall And Cellulose Biosynthesis In Model Species And In The C4 Model Plant Setaria Viridis, Mizuki Tateno

Theses and Dissertations--Plant and Soil Sciences

A uniform feature of all plant cells is the presence of a cell wall. The cell wall functions in facilitating directional expansion and is therefore important for cell shape and morphogenesis. All plant cell walls contain cellulose microfibrils embedded in a network of polysaccharides, lignin and protein. Cellulose is evolutionarily conserved and is made by all plants as well as other members of various taxonomic kingdoms. From a human perspective, the field of renewable energy has had an ever increasing interest in using the cell wall for production of renewable platform chemicals and fuels. However, the biosynthesis of these components …

Probing The Plant Cell Wall With Herbicides: A Chemical Genetics Approach, Chad B. Brabham

Theses and Dissertations--Plant and Soil Sciences

The primary cell wall is a highly organized multi-layered matrix of polysaccharides (cellulose, hemi-cellulose, and pectin). The ability of the rigid cell wall to sufficiently loosen to allow growth is a complex process that differs considerably between grasses monocots and dicots. Cellulose is the major structural component required for anisotropic cell expansion and is synthesized by CELLULOSE SYNTHASE A (CesA) proteins. Here, our objectives were two-fold: 1) dissect cell walls and cellulose biosynthesis in dicots and grasses using chemical biology and reverse genetic approaches 2) characterize and classify the inhibitory mechanisms of cellulose biosynthesis inhibitors (CBIs). A reverse genetics TILLING …

Transcriptional Regulation Of Specialized Metabolites In Arabidopsis Thaliana And Catharanthus Roseus, Craig M. Schluttenhofer

Theses and Dissertations--Plant and Soil Sciences

For millennia humans have utilized plant specialized metabolites for health benefits, fragrances, poisons, spices, and medicine. Valued metabolites are often produced in small quantities and may command high prices. Understanding when and how the plant synthesizes these compounds is important for improving their production. Phytohormone signaling cascades, such as jasmonate (JA) activate or repress transcription factors (TF) controlling expression of metabolite biosynthetic genes. TFs regulating specialized metabolite biosynthetic genes can be manipulated to engineer plants with increased metabolite production.

WRKY transcription factor are known components of both JA signaling cascades and regulation of specialized metabolism. The presence of WRKY binding …

Productivity And Biological Nitrogen Fixation Of Different Species Of Vetches (Vicia Spp.) Under The Rainfed Conditions Of West Asia, Ali Abd El Moneim, Mohan C. Saxena

IGC Proceedings (1977-2023)

The low rainfall areas of West Asia are characterized by pastoral production system primarily based on small ruminants (sheep and goats). Rangelands serve as the main source of feed. With increasing population, the demand for livestock products has increased. As a consequence, the stocking rates have soared, increasing the pressure on rangelands. Rangeland degradation has therefore been increasing threatening the sustainability of livestock production system and the livelihood of the people dependent on it. Production of additional feed is essential to reduce the pressure on rangelands. Vetches (Vicia spp.) are endemic in the area and can be potential source …

Livestock Production From Grasslands With Improved Management Compared To Traditional Management, Florian Leiber

IGC Proceedings (1977-2023)

The value of traditional versus improved management systems for grassland is discussed. The set target is the persistence of the ecological, economic and social multifunctionality of grasslands and the increase of food output from these areas without increasing parallel requirements for arable crop or energy inputs. Based on the fact that historically traditional systems were stable and sustainable in many cases, while severe damage is faced nowadays, it is argued that lot of improvement should be based on the acknowledgment of successful traditional practises. The challenge might rather be to define improvement as a contemporary development of traditional techniques. The …

Potential Of Forages In Crop Diversification And Crop Rotation, Martin H. Entz, Joanne Thiessen Martens

IGC Proceedings (1977-2023)

Redesign of agricultural systems according to ecological principles has been proposed for the development of sustainable systems. We review a wide variety of ecologically-based crop production practices that focus on forage crops in farming systems and discuss their potential role in enhancing the profitability, environmental sustainability and resilience. Crop-livestock systems that most closely mimic natural systems through appropriate integration of diverse components appear to offer the greatest potential benefits. These systems are more energy efficient and combine high productivity with low ecological footprint. Greater understanding of ecological relationships within crop-livestock systems are required to purposefully and proactively redesign agricultural systems …

Forage Seed Quality: Dormancy, Standards And Quarantine, John G. Hampton

IGC Proceedings (1977-2023)

There are many dimensions to the concept of seed quality. Viable seed which will not germinate when provided with all the requirements for germination is dormant. Forage grasses mostly exhibit non-deep physiological dormancy (PD), while physical dormancy (PY) is common in forage legumes where imbibition is prevented by the seed coat’s impermeability to water (hard seed). Methods for breaking PD and PY to allow germination testing and/or crop establishment are discussed.

In the seed industry seed quality standards are an important feature of quality assurance and may apply for seed production contracts, seed certification, seed sale and seed importing. Standards …

Quality Seed Production And Effective Marketing Systems For Development Of Grasslands, M. Philip Rolston

IGC Proceedings (1977-2023)

Seed quality is defined as (i) maintaining genetic purity using seed certification principles (generation system; isolation; previous cropping history); (ii) achieving high levels of physical purity (especially low weed seed levels) by in field weed control and seed cleaning; (iii) achieving high levels of seed germination at harvest and maintaining germination through the seed supply chain. Seed production systems must not only produce high quality seed but also achieve economic seed yields. Seed crops have to be profitable (by being high yielding) to justify the investment of time and input costs to achieve quality. This in turn requires supporting research …

Scouting Benefits And Developing Innovations In Temperate Grassland To Sustainable Agriculture Production, Walter Ayala, Ethel Barrios, Ignacio Macedo, Jorge Sawchik, José A. Terra

IGC Proceedings (1977-2023)

Agricultural intensification raises concern about land use and future effects to natural resources. The world demand for grain, meat and forest products is increasing constantly. Changes are occurring at large scale, being a compromise for policy makers to contribute to identify opportunities to readdress the productive scenario. There is available technology to reduce impacts, without restricting necessarily productivity. Grasslands provide a large series of economical and ecological benefits to the agricultural systems, being the literature exhaustive in examples. Ley-farming and cover crops technologies are good examples of environmentally sound soil management practices. The society is also worried about an improper …

Evergraze: A Partnership Between Researchers, Farmers And Advisors To Deliver Effective Grassland Management, Warwick B. Badgery, Kate Sargeant, Michael A. Friend, Scott Glyde, Paul Sanford, Ralph Behrendt, Meredith L. Mitchell, Sean Murphy, Angela Avery

IGC Proceedings (1977-2023)

The profitable and sustainable management of livestock production from grassland systems is challenging and it can be difficult to develop a research structure that addresses farmer’s needs and has acceptable rigour and on-ground impact. This paper describes the attributes of research, development and extension (RD&E) programs that are required for a successful partnership between researchers, farmers and advisors. Insights are provided from the EverGraze program that designed, tested and implemented farming systems based on perennial pastures across southern Australia. With this project farmers and advisors were involved in setting research direction, designing experiments, providing strategic guidance over the management of …

Ecologically And Socially Sustainable Livestock Development In Marginal Areas, Ilse Köhler-Rollefson

IGC Proceedings (1977-2023)

“Marginal areas” are perceived as difficult and unproductive landscapes, always under the threat of drought, desertification and poverty. Yet, both dryland and high altitude marginal areas have an extraordinary output of livestock products; in the efficiency of producing human-edible protein they far surpass more fertile areas. This productivity under adverse climatic conditions rests on sophisticated strategies and social institutions developed by pastoral communities to deal with variability in the availability of resources. It depends on the use of animal genetic resources that are adapted to make best use of local vegetation and can cope with seasonal variations in availability. Livestock …

Valuing Variability--New Perspectives On Climate Resilient Dryland Development, Saverio Kratli

IGC Proceedings (1977-2023)

Valuing Variability is a challenge to the view of the drylands as naturally vulnerable to food insecurity and poverty. It argues that improving agricultural productivity in dryland environments is possible by working with climatic uncertainty rather than seeking to control it – a view that runs contrary to decades of development practice in arid and semi-arid lands.

Participatory And Holistic Approaches With Grassland Farmers And Development Of Policies, Alain Peeters

IGC Proceedings (1977-2023)

The world is changing rapidly. Grassland farmers and ruminant livestock breeders will have to adapt to these changing environmental, social and economic conditions. Research can help them at the condition that linear ‘top-down’ technology transfer methods are abandoned for the benefit of participatory and holistic approaches. These approaches classified in the generic term of ‘Farming System Research’ consider farmers as real research partners, and they merge farmer’s and scientist’s ideas with those of other stakeholder types. They combine scientist’s and farmer’s knowledge for creating fast innovations, easily adopted by farmers since farmers themselves contribute to their design and their development. …

Sustainable Grasslands: Resolving Management Options For Livelihood And Environmental Benefits, David R. Kemp, Warwick B. Badgery, David L. Michalk

IGC Proceedings (1977-2023)

To help solve the major issues of improving livelihoods and environmental services, grassland research needs to be evaluated within the context of relevant farm systems. Treatments need to show that they not only have significant effects but that they have effects that are meaningful in the context of the relevant farm system. Research often defines an optimum criterion for management that is a single point, but that is difficult to achieve in practice, especially when there are several components in a grassland system that need to be optimised. It is argued that an appropriate criterion for optimising management is a …

Climate Change Impact And Adaptation In Temperate Grassland And Livestock Industries, Afshin Ghahramani, Andrew D. Moore

IGC Proceedings (1977-2023)

Climate is projected to have negative impact on temperate grassland and livestock productions across the globe. Moderately elevated atmospheric CO₂ in the near future is expected to increase plant photosynthetic rates but this is likely to be limited by soil nitrogen deficits. However, in Australia at least it is unlikely that positive effect of elevated CO₂ on plant production be able to offset the negative impacts of climate change. Currently there is a considerable gap between actual and achievable production and profit in Australian grazing systems and many management and genetic improvements for climate adaptation would operate by …

Climate-Smart Brachiaria For Improving Livestock Production In East Africa: Emerging Opportunities, Sita R. Ghimire, Donald M. G. Njarui, Mupenzi Mutimura, Juan A. Cardoso, Linda Johnson, Elias M. Gichangi, Suliana Teasdale, Kennedy Odokonyero, John Caradus, Idupulapati M. Rao, Appolinaire Djikeng

IGC Proceedings (1977-2023)

Brachiaria grass is an important tropical forage of African origin with desirable attributes of agricultural and environmental significance. Brachiaria has been extensively cultivated as a pasture across the tropics except in its endemic provenance of Africa. In 2013, a collaborative research program was initiated in Kenya and Rwanda with the aim of improving the availability of quality livestock feeds adapted to drought and low fertility soils using Brachiaria. The outcomes sought were increased livestock productivity leading to improved farmer income and the development of seed production opportunities. The program has identified five preferred cultivars, and four of them are currently …

Tropical Grassland Ecosystems And Climate Change, C. R. Babu, Vivek Kr. Choudhary, Vijay Kumar

IGC Proceedings (1977-2023)

Grasses are unique group of flowering plants that form the foundation for the trophic structure in terrestrial communities. The grasses are found in every conceivable habitat where plants can thrive – from sea to deserts and from wetlands to peaks of highest mountains. The grasses form a distinct biome – a major ecological formation in the global classification of vegetation.

Recent Trends In Breeding Of Tropical Grass And Forage Species, J. S. Sandhu, Dinesh Kumar, Vijay Kumar Yadav, Tejveer Singh, R. P. Sah, Auji Radhakrishna

IGC Proceedings (1977-2023)

Germplasm enrichment in major tropical grasses and their characterization for emerging environmental challenges have been major focussed area in the recent past. Breeding efforts in tropical grasses are still limited to few selected species viz. Panicum spp, Cenchrus spp, Pennisetum spp and Bracharia spp and all other grasses use of land races for varietal development through selection have been major source of improvement. The pace of breeding efforts in the tropical grasses have been slowed because of many inherent characteristics viz. apomixis, poor seed set, high photo and thermo sensitivity often creating problem in designing and implementing an effective breeding …

Sustainability, Biodiversity And Environmental Issues: A Global Perspective For Livestock Production, David L. Michalk

IGC Proceedings (1977-2023)

To meet world food demand through the 21st century, agricultural production needs to increase, but this needs to be done sustainably through increasing efficiency, optimising sufficiency and achieving consistency, such that resource use is optimised, waste reduced and environmental benefits gained. These strategies need to be developed against changing food patterns, especially a decline in per capita consumption of cereals and an increase in meat consumption as household incomes increase. Grasslands are collectively the larger group of land-based ecosystems on the planet. Their values are not always recognised, often being seen as ‘reserves’ for exploitation for urban expansion, …

Wastelands Of The Mind: The Identity Crisis Of India’S Savanna Grasslands, Abi Tamim Vanak

IGC Proceedings (1977-2023)

The idea that wastelands are unproductive continues to be pervasive and is used by various agencies to gain control over marginal landscapes and remake them for productive purposes with dire results.

Grassland And Livestock Production: The East African Case, David Miano Mwangi

IGC Proceedings (1977-2023)

Grasslands cover over 26% of the world land mass and about 80% of the agricultural area. They are the source of a livelihood for about 1 million people in the developing countries. Livestock contributes 40% of the global value of agricultural outputs. Approximately 60% of rural households keep livestock. In East Africa there are a number of grasslands. The most extensive of these are the Acacia based Savanna grasslands. Others include the sudd flooded grasslands of South Sudan and the Miobo woodlands of Tanzania. More than 60% of the livestock and wildlife in this region are found on these grasslands. …

Three Is Company: Fixing The Grazing-Land Business Conundrum, Ralph Von Kaufmann

IGC Proceedings (1977-2023)

Grazing-lands have the largest footprint on the world’s land surface area but this is not reflected in the amount of business conducted on them. The most common land use systems are very land intensive, i.e., they use a lot of land to produce not very much and few jobs. The health of the planet depends on having healthy grazing-lands but there is a perfect storm brewing in which the demand from expanding pastoral communities for more food and jobs cannot be met just by applying good grazing-land management practices. Compatible businesses must be found that can create jobs without increasing …

Tropical Grasslands--Trends, Perspectives And Future Prospects, Panjab Singh

IGC Proceedings (1977-2023)

This paper attempts a rapid summary of the present day state of tropical grassland and livestock based production systems globally, especially in tropics, in relation to recent trends, future assessment, grassland rehabilitation and management strategies, future perspectives of grassland science and development including policy imperatives.

About Sponsors, National Organizing Committee, International Grassland Congress

IGC Proceedings (1977-2023)

No abstract provided.