Plant Biology Commons^™

Basic Biotechnology, Jennifer Flak, Julie Albrecht

Plant and Soil Sciences eLibrary

An introduction to traditional cross-breeding and genetic engineering of plants, and to biotechnology.

Objectives - Basic Biotechnology

After completing Nutrition Basic Biotechnology, participants should be able to:

• Describe one similarity between traditional cross-breeding and genetic engineering of plants.
• Give two limitations that are encountered with traditional cross-breeding of plants.
• Explain why genetic engineering is not limited in the same way as traditional cross-breeding.
• Define biotechnology in broad terms.
• List three plant applications of biotechnology.
• List the three areas of nutritional profiles that have been improved with biotechnology.

Modules:

• Lesson home
• Objectives - Basic Biotechnology
• Historical Uses of Biotechnology
• Basic Understanding …

Ec00-103 Nebraska Fall-Sown Small Grain Variety Tests 2000, Lenis Alton Nelson, David D. Baltensperger, Robert N. Klein, Roger Wesley Elmore, P. Stephen Baenziger, Todd Campbell

University of Nebraska-Lincoln Extension: Historical Materials

This circular is a progress report of variety trials conducted by personnel of the Agronomy Department and the South Central, West Central and Panhandle Research and Extension Centers and their associated agricultural laboraties. The 2000 estimated winter wheat yield for Nebraska was a 36 bushels per acre from 1,750,000 harvested acres. The total production of winter wheat for the state was 63,000,000 bushels. This circular reports data from winter wheat trials conducted throughout Nebraska. Entries included varieties or hybrids and promising experimental strains from Nebraska and surrounding states and private breeders. This was the ninetenth year for privately developed varieties. …

The Avr (Effector) Proteins Hrma (Hoppsya) And Avrpto Are Secreted In Culture From Pseudomonas Syringae Pathovars Via The Hrp (Type Iii) Protein Secretion System In A Temperature- And Ph-Sensitive Manner, Karin V. Van Dijk, Derrick E. Fouts, Amos H. Rehm, Angela R. Hill, Alan Collmer, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

We present here data showing that the Avr proteins HrmA and AvrPto are secreted in culture via the native Hrp pathways from Pseudomonas syringae pathovars that produce these proteins. Moreover, their secretion is strongly affected by the temperature and pH of the culture medium. Both HrmA and AvrPto were secreted at their highest amounts when the temperature was between 18 and 22°C and when the culture medium was pH 6.0. In contrast, temperature did not affect the secretion of HrpZ. pH did affect HrpZ secretion, but not as strongly as it affected the secretion of HrmA. This finding suggests that …

Agriculture Western Australia's Centre For Cropping Systems - Northam, Agriculture Western Australia

Grain and other field crops published reports

Agriculture Western Australia's Centre for Cropping Systems in York Road, Northam, was established as a centre of excellence for research, development and extension into cropping systems.

Its role is to stimulate the contribution of ideas from individuals, groups and organisations with an interest in research, development, and extension relating to innovative, profitable and sustainable crop-based farming systems.

The Centre for Cropping Systems is part of a regional network with strong links to Agriculture Western Australia's Crop Improvement Institute based at South Perth, the Dryland Research Institute at Merredin and the Avondale Research Fann at Beverley. It also links with universities …

Reproductive Biology Of Melilotus Officinalis (L.) Lam (Fabaceae: Papilionoideae): Morphology, Seed/Ovule Ratios And Ovule Abortion Patterns, Susan Lee Schouten

Dissertations and Theses

Melilotus officinalis, an introduced herbaceous species within the Papilionoideae, has a fixed ovule abortion pattern. In its primarily single-seeded, indehiscent fruits, with an average of 6.98 ovules/fruit, this species matured the basal-most ovule in 67.63% and the second basal-most ovule in 24.22% of the fruits studied. This pattern contrasts with other legumes studied, which abort the basal-most ovules or those at either end of the fruit. Workers propose interaction between two opposing gradients--that of maternal resources from the basal end of the fruit and of fertilization from the stylar end. Several lines of evidence indicate that all ovules are …

Horticulture Research Report 1994 - 1998, Frank Wise Institute, Kununurra, Department Of Agriculture, Western Australia, D. Robinson, M. Howes, R. Randell

Horticulture research reports

This report summarises horticultural research, development and extension activities undertaken by staff from the Kununurra District Office during the period 1993 to 1998 and reported annually at the Frank Wise Institute Horticultural Research Review. The range and extent of activities presented and supported by Agriculture Western Australia with industry collaboration indicates the importance of horticulture in the economies of the Kimberley Region and Western Australia. Further support by funding bodies and agribusiness, and collaboration with other research organizations is also acknowledged. Since the early 1980's, horticultural output and value of production from the Ord River Irrigation Area (ORIA) have risen …

Comparison Of Common Cytotypes Of Andropogon Gerardii (Andropogoneae, Poaceae), Kathleen H. Keeler, Geoffrey A. Davis

School of Biological Sciences: Faculty Publications

Many plant species contain populations with more than one polyploid cytotype, but little is known of the mechanisms maintaining several cytotypes in a population. Andropogon gerardii cytotypes were compared to evaluate different models of autopolyploid cytotype coexistence. The enneaploid (90 chromosome, 9x) cytotype was found to be larger and taller than the hexaploid (60 chromosome, 6x) cytotype. Seed production is significantly more efficient in hexaploids, but seed production per area was not significantly different. The two cytotypes are not exomorphologically separable in the field because of great plasticity in response to environmental variation and wide variation …

The Pseudomonas Syringae Pv. Tomato Hrpw Protein Has Domains Similar To Harpins And Pectate Lyases And Can Elicit The Plant Hypersensitive Response And Bind To Pectate, Amy O. Charkowski, James R. Alfano, Gail Preston, Jing Yuan, Sheng Yang He, Alan Collmer

Center for Plant Science Innovation: Faculty Publications

The host-specific plant pathogen Pseudomonas syringae elicits the hypersensitive response (HR) in nonhost plants and secretes the HrpZ harpin in culture via the Hrp (type III) secretion system. Previous genetic evidence suggested the existence of another harpin gene in the P. syringae genome. hrpW was found in a region adjacent to the hrp cluster in P. syringae pv. tomato DC3000. hrpW encodes a 42.9-kDa protein with domains resembling harpins and pectate lyases (Pels), respectively. HrpW has key properties of harpins. It is heat stable and glycine rich, lacks cysteine, is secreted by the Hrp system, and is able to elicit …

Intrapopulation Sex Ratio Variation In The Salt Grass Distichlis Spicata, Sarah M. Eppley, Maureen L. Stanton, Richard K. Grosberg

Biology Faculty Publications and Presentations

In many dioecious plant populations, males and females appear to be spatially segregated, a pattern that is difficult to explain given its potentially high costs. However, in asexually propagating species, spatial segregation of the sexes may be indistinguishable from superficially similar patterns generated by random establishment of a few genets followed by extensive clonal spread and by gender-specific differences in rates of clonal spread. In populations where a significant fraction of individuals are not flowering and gender cannot be assigned to this fraction, apparent spatial segregation of the sexes may be due to differential flowering between the sexes. We confirm …

Genetic Markers In Plant Evolutionary Ecology, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Genetic markers have provided plant ecologists with a method of assessing levels of genetic relatedness among individuals and populations. In recent years a number of techniques based on DNA sequence variation have been developed to complement allozyme methods that are already widely used. Some of these new markers are more variable than protein-based markers, allowing more precise estimates of genetic differences among individuals and populations. Other DNA-based markers are based on organelle genomes that are inherited uniparentally. These cytoplasmic markers can provide a method for assessing the separate effects of seed and pollen dispersal on gene flow within and among …

Evolutionary Implications Of Meiotic Chromosome Behavior, Reproductive Biology, And Hybridization In 6x And 9x Cytotypes Of Andropogon Gerardii (Poaceae), Guillermo A. Norrmann, Camilo L. Quarín, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

Andropogon gerardii, big bluestem, has 60 and 90 chromosome cytotypes. Meiosis in the hexaploid was shown to be regular, although some secondary associations of bivalents form. Meiosis in the enneaploid (2n = 9x = 90) is irregular, leading to most gametes having unbalanced chromosome complements. Both cytotypes show considerable self-incompatibility. Cytotypes crossed freely, forming a variety of fertile euploids and aneuploids. Indistinguishable exomorphology, intermixing in natural populations, and compatibility suggest that A. gerardii is best understood as a cytotypically complex single species.

Movement Of Crop Transgenes Into Wild Plants, Kathleen H. Keeler, Charles E. Turner, Margaret R. Bolick

School of Biological Sciences: Faculty Publications

Despite the great potential and increasing importance of other weed control options (Turner et al. 1992) and unwanted environmental side effects of some herbicides, herbicides constitute a very important means of weed control. The escape of herbicide resistance genes to wild, weedy plants could cause more severe weed problems, and presents a very real threat to the efficacy of herbicides as a weed control option. Therefore, management strategies that prevent, or reduce the likelihood and frequency of HRG escape through containment methods are advisable, as are mitigation plans in the event of HRG escape to wild plants.

Remnant Vegetation And Natural Resources Of The Blackwood River Catchment An Atlas, Shaun B. Grein

Agriculture reports

The Blackwood River is one of the longest rivers in the South-West of Western Australia, stretching 300 km from Moordjarrup to August. The catchment covers more than 28,000 square kilometres (2.8 million ha) from the Shire of Kent to the Shire of Augusta-Margaret River. It incorporates 17 shires, more than 30,000 people, 18 Land Conservation District Committees (LCDCs) and 143 sub-catchment groups. Shires within the Blackwood Catchment cover 4.12 million hectares, over half of the total area of the shires that fall within the catchment boundary.

The Analysis Of Genetically And Physiologically Complex Traits Using Ceratopteris: A Case Study Of Nacl-Tolerant Mutants, Thomas R. Warne, Dale L. Vogelien, Leslie G. Hickok

Faculty Articles

Genetic and physiological complexities associated with salt tolerance in plants have limited progress in the analysis of specific factors responsible for the salt-tolerant phenotype. We have used the homosporous fern Ceratopteris richardii as a model plant to investigate the physiological basis of salinity tolerance by selecting single gene mutants that confer tolerance in the gametophyte generation. The unique genetic system of homosporous ferns permits the generation of mutants in a genetic background nearly isogenic to the wildtype, such that comparative studies with the wildtype can identify specific physiological responses associated with salt tolerance. One of these mutations, stl2, confers …

Adaptation Of Lentil In Western Australia: Germplasm Evaluation In 1994, A. Sarker, K. H.M. Siddique, S P. Loss, Co-Operative Research Centre For Legumes In Mediterranean Agriculture, Department Of Agriculture Western Australia

Grain and other field crops published reports

Lentil is one of the grain legumes with potential for inclusion into Australian farming systems on fine textured, neutral to alkaline soils. It is an important protein source for the large populations of the Indian sub-continent and elsewhere, and the export market potential is excellent. Old Canadian varieties grown on a small scale in Victoria (Laird and Callisto) are poorly adapted to short season WA environments, A number of varieties have recently been selected from ICARDA material and released in Victoria and SA (Digger, Cobber, Matilda and Aldinga), and these have adequate adaptation to commence a lentil industry in WA. …

Grain Legume And Canola Autumn Updates 1995, David Williams

Grain and other field crops published reports

Grain legumes

- Chickpea

- Field pea

- Lentil

- Faba beans

Canola essentials:

Site selection -Paddock selection is critical for a successful canola crop. Avoid paddocks with waterlogging, wind erosion and broadleaf weeds - particularly wild radish, turnip, mustard and doublegees.

Soil type - Canela grows well on most soil types, however it prefers well drained gravel soils, red loams, sandy _loams and deeper duplex soils. Soil pH levels (in CaCl_²) of 4.5 to 8.0 are suitable. Soil test paddock for pH, phosphorus, and potassium levels.

Rotation - Grow canola after manipulated pasture, lupins or other grain …

Growing Lettuce, Margaret Graham

Horticulture published reports

The commonly cultivated lettuce, Lactuca sativa, is an annual belonging to the sunflower family. It has been cultivated for over 2500 years, and is a popular salad vegetable.

There are three main subspecies commonly grown.

• IL. sativa ssp capitata - Head type - Crisphead is a lettuce with a firm head and brittle leaves. It is the major lettuce type grown in Australia and is also known as iceberg lettuce. - Butterhead (buttercrunch, mignonette) lettuce has soft, pale leaves and a delicate buttery flavour. It is of major importance in Europe, where it is grown as a greenhouse crop.
• L. …

Gascoyne Research Station Field Day, Department Of Agriculture, Western Australia

Horticulture research reports

Gascoyne Research Station Centenary Open Day Thursday 13 October 1994

100 years (1894-1994) Advancing Agriculture

Welcome to the 1994 Field Day at Gascoyne Research Station. At this day you'll have the opportunity to view and hear discussions of some of the latest Research and Development initiatives in horticulture in the region. We will commence activities at the Packing Shed at 3:00 p.m. with introductions and short discussions of new work which is currently in progress. This will be followed by field talks at the trial sites, as listed in this brochure. We are also pleased to include presentations by leaders …

Fast Tracking Barley Varieties Using Anther Culture, Sue Broughton, Penny Priest

Journal of the Department of Agriculture, Western Australia, Series 4

Breeding new varieties of barley or other cereal crops usually takes between 12 and 15 years. five years of that time may be needed to stabalise the new varieties so that they breed trueto type, but anther culture can reduce this delay to only eight months. This technology will allow the Department of Agriculture's barley breeding programme to respond more rapidly to changes in goals set by industry and to meet market demands.

Remnant Vegetation Protection Scheme 1991 Blocks, J E D Fox, P Read, J - Pg Orsini

All other publications

No abstract provided.

New Yellow Serradella Varieties For Low Rainfall Pastures, Clinton Revell

Journal of the Department of Agriculture, Western Australia, Series 4

Large areas of acidic, sandy soils in Western Australia's low and medium rainfall, wheatsheep areas are suited to the pasture legume, yellow serradella.

In the past, a lack of varieties with suitable maturity has limited the use of this species.

New varieties developed in Western Australia and significantly earlier in maturity than traditional types can now extend the use of yellow serradella into these regions.

Local Polyploid Variation In The Native Prairie Grass Andropogon Gerardii, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

The microscale distribution of polyploid variants of the dominant grass big bluestem (Andropogon gerardii) in virgin tallgrass prairie was mapped using flow cytometry. The correlation between DNA content and polyploidy allows the use of flow cytometry for nondestructive determination of polyploidy in intact plants. At Konza Prairie, local plots contained from 0 to 100% hexaploid cytotypes but most showed fine-scale mixing of the polyploid variants. The relationship of cytotype frequency to moisture availability or burning history was nonsignificant

1991 Catalogue Of The Australian Lupin Collection Including Field Evaluation Data For Wild, Semi-Domesticated And Fully Domesticated Accessions, J. C. Clements, W. A. Cowling

Grain and other field crops research reports

This report lists all wild, semi-domesticated and fully domesticated accessions in the Australian Lupin Collection to 1991. The Collection is composed of 2,264 accessions (Perth Numbers between 20629 and 27067) introduced to Australia or from naturalized populations and breeding programmes within Australia. Several accessions are selections from variable populations which were identified during field trials in South Perth, Western Australia. This catalogue lists the characteristics and field evaluation data for every accession where available. Plant breeders and crop scientists will find this information useful for the selection of lupin genotypes with desired characteristics.

Section A of this catalogue should be …

Bulletin No 4220 - Growing High Yielding Wheat Crops In The Central Wheatbelt, Michael Perry

Bulletins 4000 -

In this Bulletin we hope to provide you with what we believe is the best and most up-to-date information about growing wheat in your particular environment.

It is now more important than ever for producers to get the most out of their resources of land, labour and capital. The key to doing that is the knowledge to do even better what you probably do well already-growing wheat.

Origin And Evolution Of Defective Interfering Rnas Of Tomato Bushy Stunt Virus, David A. Knorr, Thomas Jack Morris

School of Biological Sciences: Faculty Publications

Viruses with defective genomes have been identified in association with virtually every major family of viruses and have been widely utilized as tools for investigating virus functions in animal cell culture systems (Perrault, 1981). It is generally thought that defective interfering viruses (DIs) arise through deletion, rearrangement, or recombination of a competent viral genome. DIs tack the ability for independent existence relying on their parental helper viruses to supply factors required for replication, maturation, and/or encapsidation (Huang and Baltimore, 1977). The interference attributed to DIs is thought to result from competition with the helper virus for factors required in trans …

Sexual Dimorphism And Intersexual Differences In Resource Allocations Of A Dioecious Shrub, Lindera Melissifolia (Walt.) Blume, Dennis J. Richardson, Robert D. Wright, Shannon Walker

Journal of the Arkansas Academy of Science

Morphometric data were gathered to make intersexual comparisons on the morphology and reproductive resource allocation patterns of the rare, dioecious shrub, Lindera melissifolia. Females produced significantly fewer flowers and leaves than did males. No other significant differences could be detected in morphology. Female "clones" exhibit low area coverage and low stem density, which suggests higher costs of reproduction. If this is the case, the deficits produced in resource allocations directed toward sexual reproduction seem to result in increased mortality and/or reduced vegetative reproduction.

Early Sowing Of Ceral Crops In Low Rainfall Areas, R J. Delane, John Hamblin

Journal of the Department of Agriculture, Western Australia, Series 4

One of the objectives of the Department of Agriculture's crop research in the low rainfall, northern wheatbelt is to develop crop varieties and management practices that will make best use of stored water on both sandplain and fine textured soils. Sowing a crop early will improve its water use efficiency and yiel in low rainfall areas.

Introducing Onslow, Western Australian Department Of Agriculture

Grain and other field crops published reports

Onslow Barley........ a new breed of barley for the southern high rainfall areas (see map VH,H4 &H5). Suited to May plantings, Onslow is the first barley developed specifically for the long growing seasons in these areas.

For the first time barley growers have a variety designed for those 4 t/ha plus yields which research shows are possible with early sowing.

Onslow is the first of a new breed of barley cultivars developed in Western Australia specifically for the southern high rainfall areas which have a long growing season.

Looking At Lupins - Lupin Management In The South Eastern Wheatbelt, W. L. Smart, M. M. Ralph, J. M. Liddle, R.. D. Ramm, C. J. Robinson, E. W. Armstrong

Grain and other field crops research reports

In the past couple of years research into lupins has increased substantially particularly in the fields of weed control, seeding rates, and control of diseases such as brown leaf spot and pleiochaeta root rot. This report is a summary of the lupin trials in the Lake Grace advisory area as well as a guide to better lupin farming.

Jamaican Blue-Green Algae Collections Of J.C. Strickland, W. John Hayden

Biology Faculty Publications

Professor John C. Strickland (1915-1980) devoted much of his life to teaching biology, botany, and phycology at the University of Richmond. Throughout his academic career he maintained a keen interest in the Myxophyceae, or blue-green algae, studying their culture, cytology, and taxonomy (Drouet & Strickland, 1942; Strickland, 1940, 1946). Most of his collections of these and other algae were made in Virginia and are housed in the herbarium maintained by the Department of Biology, University of Richmond. However, he also made four trips to Jamaica in the years 1966-1971 before his health deteriorated to the extent that field work, and …