Botany Commons^™

Red-Bellied Snake (Storeria Occipitomaculata) Copulation In South Dakota, Drew R. Davis

The Prairie Naturalist

Storeria occipitomaculata is a small, terrestrial species of snake that occurs across much of eastern North America (Ernst and Barbour 1989, Ernst 2002, Ernst and Ernst 2003). Due to this widespread distribution, S. occipitomaculata faces varied climates that likely result in regional differences in reproductive phenology. Much of what is known about the reproductive ecology of S. occipitomaculata has been documented from the southeastern portion of its range in North America (South Carolina: Semlitsch and Moran 1984, North Carolina: Willson and Dorcas 2004), though Blanchard (1937) studied a population from northern Michigan. The exact reproductive timing in this …

Understanding Spatial Dynamics Of Tallgrass Prairie Dominated By Tall Fescue, Callie Griffith

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

This study was conducted on restored tallgrass prairie and invaded tallgrass prairie located in the Grand River Grasslands of southern Iowa to determine differences in heterogeneity of plant structure and functional group composition at different scales. Restored tallgrass prairies were seeded with a species-rich seeding mixture and managed by burning the entire prairie, every three years. Data were collected in August 2014 and 2015 to compare heterogeneity of restoration of native plant structure and functional group composition to the heterogeneity of invaded tallgrass prairies that were managed with patch burning and conventionally grazed. Invaded tallgrass prairies were managed with patch-burning …

Grazing Strategy Effects On Utilization, Animal Performance, Aboveground Production, Species Composition, And Soil Properties On Nebraska Sandhills Meadow, Aaron Shropshire

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Ultrahigh stocking density (a.k.a., mob grazing) is proposed as a management tool that results in greater harvest efficiency, animal performance, aboveground plant production, species richness, and soil carbon content. The study objective was to determine grazing treatment, haying, or non-defoliated control effects on forage utilization, aboveground production, animal performance, and soil properties. In 2010, 25 ha of Sandhills meadow were divided into 2 replications of 3 grazing, a hay, and control treatment. Grazing treatments were a 120-pasture rotation with one grazing cycle (mob), a 4-pasture rotation with one cycle (4PR1), and a 4-pasture rotation with two cycles (4PR2) at stocking …

Review: North American Ducks, Geese & Swans: Identification Guide. Frank S. Todd., Kevin M. Ringelman

The Prairie Naturalist

The North American Duck, Geese & Swans: Identification Guide, released in 2018, is the culmination of a lifetime of waterfowl photography by the late Frank Todd. Indeed, this book stands apart from other identification guides as being entirely photo-driven, with minimal introductory material, notations of field marks, or descriptions of the various waterfowl species. It is small enough to be carried in the field (6.5” × 9” × 0.5”), but most readers will find it less useful than a standard bird identification book (Sibley 2014), even for waterfowl. As the title suggests, the Todd guide focuses on North American species, …

Preparing An Effective Poster Presentation, Christopher N. Jacques

The Prairie Naturalist

Greetings GPNSS members! By the time you read this editorial, many of have been experiencing the fury unleashed by ‘Old Man Winter’ and may find yourself counting the days until warmer spring temperatures return once again to the Great Plains. Until then, just a couple more months of bitter cold temperatures, strong winds, and blowing and drifting snow. But not to worry, winter also provides the cold weather enthusiasts among us a chance to enjoy a range of outdoor recreational opportunities, a chance to reflect on the previous year in review, exciting professional and personal opportunities ushered in by the …

Comparison Of Northern Flying And Red Squirrel Phylogenies With Focus On The Insular United States, Alyssa M. Kiesow, Hugh B. Britten

The Prairie Naturalist

Northern flying squirrel (Glaucomys sabrinus) and red squirrel (Tamiasciurus hudsonicus) populations are endemic to northern North America, including the Black Hills. The Black Hills populations are considered disjunct from other populations within their range. We examined insular populations to determine whether arboreal squirrels in the Black Hills each represent a unique population. We trapped and collected ear samples from northern flying and red squirrels in the Black Hills and in areas of Montana, Wyoming, Idaho, Utah, Minnesota, and Wisconsin to infer population phylogenies with special consideration of the Black Hills population. Microsatellite loci and two mtDNA sequences were used for …

Notes: Two-Headed White-Tailed Deer Fetus, William F. Jensen, Daniel M. Grove, Ryan J. Herigstad, William J. Haase

The Prairie Naturalist

On 6 April 2018 a female white-tailed deer (Odocoileus virginianus dakotensis) was hit and killed by a vehicle along HWY 1806 in rural Morton County, North Dakota, USA (N46o 38.617; W100o 42.901). Based upon dental eruption and wear (Severinghaus 1949), the female was estimated to be a >4.5 years-of-age. A male fetus with parapagus diprosopus (i.e., shared face) apparently was expelled from the adult female, and a domestic canine (Canis lupus familiaris) carried the fetus to a private residence. The resident contacted the North Dakota Game and Fish Department (NDGFD) to turn in the fetus. On 10 April 2018, NDGFD …

Metabolic Gas Emissions From Prairie Soil Containing Foraging Termites, Charles E. Konemann, B.M. Kard, Tom A. Royer, Mark E. Payton

The Prairie Naturalist

Differences in subterranean termite metabolic gas emissions are readily observed in laboratory experiments. However, in natural field ecosystems a primary difficulty in measuring subterranean termite gases is non-homogeneous distribution of foraging termites in soil. Our field experiment was designed to aggregate foragers of the 'eastern subterranean termite', Reticulitermes flavipes Kollar (EST), in one of four flux chamber configurations placed on a tallgrass prairie throughout 2014 and 2015. We used differently configured flux chambers to measure metabolic gas emissions from soil with or without foraging termites on The Joseph H. Williams Tallgrass Prairie Preserve (TGPP) in north-central Oklahoma. Foraging termitesaggregated in …

The Prairie Naturalist: The Journal Of The Great Plains Natural Science Society Volume 50 No. 2

The Prairie Naturalist

TABLE OF CONTENTS

43 EDITOR’S NOTE

RESEARCH ARTICLES

46 Metabolic Gas Emissions from Prairie Soil Containing Foraging Termites Charles E. Konemann, B. M. Kard, Tom A. Royer, and Mark. E. Payton

59 Comparison of northern flying and red squirrel phylogenies with focus on the insular United States Alyssa M. Kiesow, and Hugh B. Britten

NOTES

70 Two-Headed White-Tailed Deer Fetus

72 Red-Bellied Snake (Storeria occipitomacilata) Copulation in South Dakota

74 New Breeding Record and Location for Wilson's Phalarope (Phalaropus tricolor) in the Nebraska Great Plains, USA

BOOK REVIEWS

76 Sedges and Rushes of Minnesota: The Complete Guide to Species Identification. …

Investigation Of Alternative Hosts And Agronomic Factors Affecting Xanthomonas Vasicola Pv. Vasculorum, Causal Agent Of Bacterial Leaf Streak Of Corn, Terra Marie Hartman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Xanthomonas vasicola pv. vasculorum, causal agent of bacterial leaf streak of corn, was first reported in the U.S. in 2016 on Nebraska corn leaf samples. Prior to this report, the pathogen had only been reported on corn in South Africa. After the Nebraska report, the pathogen was reported on corn in Argentina, Brazil, and several U.S. states. This pathogen has an extensive host range, which includes sugarcane (Saccharum officinarum), grain sorghum (Sorghum bicolor), palm species (Dictyosperma album, Roystonea regia, and Areca catechu) and broom bamboo (Thysanolaena maxima). Plants commonly found in …

Identification Of Genes/Genomic Regions Controlling Resistance To Biotic And Abiotic Stresses In Synthetic Hexaploid Wheat, Madhav Bhatta

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Synthetic hexaploid wheat (SHW; 2n=6x=42, AABBDD, Triticum aestivumL.) is produced from an interspecific cross between durum wheat (2n=4x=28, AABB, T. turgidumL.) and goat grass (2n=2x=14, DD, AegilopstauschiiCoss.). It is reported to have a considerable amount of genetic diversity and is a potential source of novel alleles controlling abiotic and biotic stresses resistance and improving wheat quality. Therefore, the first study was to understand the genetic diversity and population structure of SHWs and compare the genetic diversity of SHWs with elite bread wheat (BW) cultivars. The result of this study identified a wide range of genetic diversity within …

Canavalia Gladiata And Dolichos Lablab Extracts For Sustainable Pest Biocontrol And Plant Nutrition Improvement In El Salvador, Carlos Martinez

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Botanical repellents and pesticides are now being rediscovered as new tools for integrated pest management in order to reduce the use of toxic chemicals in crop production. Canavalia gladiata and Dolichos lablab are two Fabaceae very well adapted to farmlands of El Salvador, effective as living barriers and mostly as cover crops, however, they are not yet very well disseminated. This document describes the potential for using the liquid extracts and the dry flour of raw seeds of those plants for economic benefit and practical convenience for pest management in Salvadorian agriculture under field conditions. Seed extracts were useful when …

Assessment Of Soil Fertility Under Different Land-Use Systems In Dhading District Of Nepal, Sudarshan Kharal, Babu Ram Khanal, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

Unscientific land use and cropping techniques have led high soil erosion and degradation of soil quality in the mid-hills of Nepal. To understand the effects of land use systems for selected soil chemical properties in mid-hills, composite soil samples at 0 cm to 20 cm depth were collected from five different land-use systems: Grassland, forest land, upland, lowland, and vegetable farms from Dhading district of Nepal in 2017. Soil samples were analyzed for soil fertility parameters: Soil pH, organic matter (OM), total nitrogen (N), available phosphorus (P), available potassium (K) and its effect due to different land use systems were …

Research Report: Prevalence And Mechanism Of Atrazine Resistance In Waterhemp From Nebraska, Rodrigo Werle, Mithila Jugulam, Greg Kruger, Amaranatha Vennapusa, Felipe Faleco, Bruno Viera, Spencer Samuelson

Department of Agronomy and Horticulture: Faculty Publications

Waterhemp is a troublesome summer annual broadleaf weed species that has evolved resistance to glyphosate and other herbicide sites of action (SOA) in Nebraska, including to groups 2 (i.e., Classic, Pursuit, FirstRate), 4 (i.e., 2, 4-D), 5(i.e., atrazine) and 27 (e.g., Callisto, Laudis). The overall objectives of this study were to

1. Evaluate the eficay of PRE applied atrazine, metribuzin and sulfentrazone to control Nebraska waterhemp populations;
2. Evaluate the efficacy of POST applied atrazine to control Nebraska waterhemp populations; and
3. Determine the mechanism of atrazine resistance in Nebraska waterhemp populations.

Digital Soil Mapping In The Bara District Of Nepal Using Kriging Tool In Arcgis, Dinesh Panday, Bijesh Maharjan, Devraj Chalise, Ram Kumar Shrestha, Bikesh Twanabasu

Department of Agronomy and Horticulture: Faculty Publications

Digital soil mapping has been widely used to develop statistical models of the relationships between environmental variables and soil attributes. This study aimed at determining and mapping the spatial distribution of the variability in soil chemical properties of the agricultural floodplain lands of the Bara district in Nepal. The study was carried out in 23 Village Development Committees with 12,516 ha total area, in the southern part of the Bara district. A total of 109 surface soil samples (0 to 15 cm depth) were collected and analyzed for pH, organic matter (OM), nitrogen (N), phosphorus (P, expressed as P_{2 …}

Overexpression Of The Sorghum Bicolor Sbccoaomt Alters Cell Wall Associated Hydroxycinnamoyl Groups, Hannah M. Tetreault, Erin D. Scully, Tammy Gries, Nathan A. Palmer, Deanna L. Funnell-Harris, Bruce S. Dien, Gautam Sarath, Thomas E. Clemente, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

Sorghum (Sorghum bicolor) is a drought tolerant crop, which is being developed as a bioenergy feedstock. The monolignol biosynthesis pathway is a major focus for altering the abundance and composition of lignin. Caffeoyl coenzyme-A O-methyltransferase (CCoAOMT) is an S-adenosyl methionine (SAM)-dependent O-methyltransferase that methylates caffeoyl-CoA to generate feruloyl-CoA, an intermediate required for the biosynthesis of both G- and S-lignin. SbCCoAOMT was overexpressed to assess the impact of increasing the amount of this enzyme on biomass composition. SbCCoAOMT overexpression increased both soluble and cell wall-bound (esterified) ferulic and sinapic acids, however lignin concentration and its composition …

Elucidating Sorghum Biomass, Nitrogen And Chlorophyll Contents With Spectral And Morphological Traits Derived From Unmanned Aircraft System, Jiating Li, Yeyin Shi, Arun-Narenthiran Veeranampalayam-Sivakumar, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Unmanned aircraft systems (UAS) provide an efficient way to phenotype cropmorphology with spectral traits such as plant height, canopy cover and various vegetation indices (VIs) providing information to elucidate genotypic responses to the environment. In this study, we investigated the potential use of UAS-derived traits to elucidate biomass, nitrogen and chlorophyll content in sorghum under nitrogen stress treatments. A nitrogen stress trial located in Nebraska, USA, contained 24 different sorghum lines, 2 nitrogen treatments and 8 replications, for a total of 384 plots. Morphological and spectral traits including plant height, canopy cover and various VIs were derived from UAS flights …

The Effect Of Ingredient-Specific Calorie Information On Calories Ordered, Christopher R. Gustafson, Eliana Zeballos

Department of Agronomy and Horticulture: Faculty Publications

Providing calorie labeling is a widely used strategy to combat obesity. However, there is little evidence that the availability of calorie information at food-away-from-home establishments has an effect on food choices. Listing calorie information for each ingredient, though, may allow customers to avoid high-calorie items that add little to their enjoyment. Data from a natural experiment were used to compare total calories ordered before and after the provision of per-ingredient versus for build-your-own sandwiches, and per-sandwich for pre-defined sandwiches, at a supermarket sandwich counter. Sandwich order slips from a Lincoln, Neb. supermarket were collected from December 15, 2016 to February …

Phylogenomic Evidence For Ancient Recombination Between Plastid Genomes Of The Cupressus-Juniperus-Xanthocyparis Complex (Cupressaceae), Andan Zhu, Weishu Fan, Robert P. Adams, Jeffrey P. Mower

Department of Agronomy and Horticulture: Faculty Publications

Background: Phylogenetic relationships among Eastern Hemisphere cypresses, Western Hemisphere cypresses, junipers, and their closest relatives are controversial, and generic delimitations have been in flux for the past decade. To address relationships and attempt to produce a more robust classification, we sequenced 11 new plastid genomes (plastomes) from the five variously described genera in this complex (Callitropsis, Cupressus, Hesperocyparis, Juniperus, and Xanthocyparis) and compared them with additional plastomes from diverse members of Cupressaceae.

Results: Phylogenetic analysis of protein-coding genes recovered a topology in which Juniperus is sister to Cupressus, whereas a tree based on …

Mobile Elements Shape Plastome Evolution In Ferns, Tanner A. Robison, Amanda L. Grusz, Paul G. Wolf, Jeffrey P. Mower, Blake D. Fauskee, Karla Sosa, Eric Schuettpelz

Department of Agronomy and Horticulture: Faculty Publications

Plastid genomes display remarkable organizational stability over evolutionary time. From green algae to angiosperms, most plastid genomes are largely collinear, with only a few cases of inversion, gene loss, or, in extremely rare cases, gene addition. These plastome insertions are mostly clade-specific and are typically of nuclear or mitochondrial origin. Here, we expand on these findings and present the first family-level survey of plastome evolution in ferns, revealing a novel suite of dynamic mobile elements. Comparative plastome analyses of the Preridaceae expose several mobile open reading frames that vary in sequence length, insertion site, and configuration among sampled taxa. Even …

Evaluation Of Glyphosate And Ppo-Inhibiting Herbicide Tank-Mixtures To Manage Glyphosate Resistance In Soybean, Jesaelen Gizotti De Moraes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Protoporphyrinogen oxidase (PPO)-inhibiting herbicides in combination with glyphosate for postemergence (POST) applications is one of the primary alternatives to manage glyphosate-resistant weeds and the only effective POST chemical option in conventional and glyphosate-tolerant soybean to control glyphosate and ALS-inhibiting resistant weeds. Antagonistic interactions have been reported between many different herbicide modes of action and optimal droplet size may be affected by tank-mixtures of different herbicides. Additionally, the impact of adjuvants on the factors aforementioned as well as on physical properties needs to be thoroughly investigate to maximize herbicide efficacy. Therefore, the objectives of this research were to: 1) conduct greenhouse …

Utilizing Random Regression Models For Genomic Prediction Of A Longitudinal Trait Derived From High‐Throughput Phenotyping, Malachy T. Campbell, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

The accessibility of high‐throughput phenotyping platforms in both the greenhouse and field, as well as the relatively low cost of unmanned aerial vehicles, has provided researchers with an effective means to characterize large populations throughout the growing season. These longitudinal phenotypes can provide important insight into plant development and responses to the environment. Despite the growing use of these new phenotyping approaches in plant breeding, the use of genomic prediction models for longitudinal phenotypes is limited in major crop species. The objective of this study was to demonstrate the utility of random regression (RR) models using Legendre polynomials for genomic …

Identification Of Genomic Regions Contributing To Protein Accumulation In Wheat Under Well-Watered And Water Deficit Growth Conditions, Ibrahim S. Elbasyoni, Sabah M. Morsy, Raghuprakash Kastoori Ramamurthy, Atef M. Nassar

Department of Agronomy and Horticulture: Faculty Publications

Sustaining wheat production under low-input conditions through development and identifying genotypes with enhanced nutritional quality are two current concerns of wheat breeders. Wheat grain total protein content, to no small extent, determines the economic and nutritive value of wheat. Therefore, the objectives of this study are to identify accessions with high and low grain protein content (GPC) under well-watered and water-deficit growth conditions and to locate genomic regions that contribute to GPC accumulation. Spring wheat grains obtained from 2111 accessions that were grown under well-watered and water-deficit conditions were assessed for GPC using near-infrared spectroscopy (NIR). Results indicated significant influences …

The Effects Of Fungicide And Genetics On Fungal Diseases On Wheat In Nebraska With Emphasis On Stem Rust, Javed Sidiqi

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Foliar fungal diseases of wheat (Triticum aestivum L.) pose a serious threat to wheat production. Despite the significant progress that has been made limiting major fungal diseases by developing resistant cultivars, these diseases challenge the economic sustainability of wheat production. The objectives of this research were (1) To determine the effect of fungal plant pathogens on grain yield in eastern Nebraska, released and pre-release winter wheat genotypes were studied in fungicide treated and untreated plots from 2015 to 2017. (2) To determine if Sr7b is present using allelism tests in ‘Gage’ wheat as previously hypothesized.

Sixty genotypes were planted …

Field Pea Response To Seeding Rate, Depth, And Inoculant In West-Central Nebraska, Strahinja V. Stepanovic, Charles Burr, Julie A. Peterson, Daran Rudnick, Cody F. Creech, Rodrigo Werle

West Central Research and Extension Center, North Platte

Increased market demand and larger adoption of field pea (Pisum sativum L.) in semiarid west-central Nebraska has provided opportunities to replace summer fallow and diversify crop rotations. As a relatively new crop, its response to different seeding practices has not been evaluated in this eco-region. Field pea grain yield response to seeding depth (25, 50, and 75 mm), inoculation with Rhizobium leguminosarum bv. viciae (yes and no rhizobia inoculant), and seeding rates (35, 50, 65, 75, 90, 105, and 120 plants m^–2) was investigated in 2015 and 2016 at five sites in Perkins County, NE. There were …

Lekking Behavior Of A Sharp-Tailed Grouse In South-Central Nebraska, Kelsey C. King, Andrew J. Caven, Keith Geluso

The Prairie Naturalist

Sharp-tailed grouse (Tympanuchus phasianellus; STGR) occur throughout much of the northern Great Plains, and historically were suspected to have ranged as far south as Kansas (Connelly et al. 1998). Sharp-tailed grouse were the dominant grouse in Nebraska 200 years ago, but early settlers and their associated agricultural practices extirpated STGR from much of their former range, including Kansas and southern Nebraska (Sisson 1976, Johnsgard 2016). During the period of initial land conversion, the greater prairie-chicken (T. cupido; GRPC) filled the void, benefiting from small plots of cropland providing winter forage, and outcompeting the STGR (Johnsgard and Wood 1968, Svedarsky et …

A Short Narrative For Writing For The Prairie Naturalist, Christopher N. Jacques

The Prairie Naturalist

The abstract should be written upon completion of the paper. A well-written abstract is a single short (≤1 line/page of text), concise, and includes 1) an introductory sentence justifying why the study was conducted, 2) a statement of the principal objectives or hypotheses tested during the study, 3) a brief description of pertinent methods, 4) a summary of significant results, 5) a punchy conclusion, and 6) management implications (i.e., utility of results explaining how, when, where, and by whom data or interpretations can be applied; Krausman and Cox 2017). Keep in mind that abstracts are read more than authors than …

The Prairie Naturalist: The Journal Of The Great Plains Natural Science Society Volume 50 No. 1

The Prairie Naturalist

TABLE OF CONTENTS

1 EDITOR’S NOTE

RESEARCH ARTICLES

3 Spatiotemporal Distribution of Waterfowl Disease Outbreaks in Kansas, USA Thomas A. Becker, Adam A. Ahlers, Shane Hesting, and David A. Haukos

14 Wild Turkey Occupancy in a Prairie Landscape Joshua C. Courlas and R. Scott Lutz

24 Age and Growth of Cottonwood Trees along the Missouri River, North Dakota Jonathan M. Friedman, Fisher R. Ankeny, and Marshall Wolf

NOTES

34 Hoary Bat Impaled on Barbed Wire Fence

37 Lekking Behavior of a Sharp-Tailed Grouse in South-Central Nebraska

BOOK REVIEWS

40 Force of Nature: George Fell, Founder of The Natural Areas Movement. …

Age And Growth Of Cottonwood Trees Along The Missouri River, North Dakota, Jonathan M. Friedman, Fisher R. Ankney, J. Marshall Wolf

The Prairie Naturalist

The relict plains cottonwood (Populus deltoides subsp. monilifera) forest along the Missouri River between Lakes Sakakawea and Oahe includes trees as large as two meters in diameter. We cored 24 of these trees to determine their age and suitability for flow reconstruction. Because most of the trees were rotten in the center, we developed a method to estimate the date of the center ring that accounts for the increase in ring width toward the center. Estimated center ring dates were as early as 1806. Cottonwood growth at a dry site was correlated with April–August flow prior to construction of Lake …

Wild Turkey Occupancy In A Prairie Landscape, Joshua C. Courlas, R. Scott Lutz

The Prairie Naturalist

We investigated wild turkey (Meleagris gallopavo) distribution in prairie landscapes in North Dakota using occupancy modeling in two stages. In 2012, we extensively surveyed ecoregions across the state and in 2013, intensively surveyed the ecoregion with the highest probability of occupancy. Occupancy models from the statewide survey indicated wild turkeys were sparse in ecoregions with primarily agricultural landscapes, were found associated with wooded riparian cover, and found most frequently in the Missouri River Plateau ecoregion. In the Missouri River Plateau, our occupancy models identified that an additive model including mean patch area of cropland fields and spatial aggregation of forest …