Botany Commons^™

Adaptive Capacity In Ecosystems, David G. Angeler, Hannah Fried-Petersen, Craig R. Allen, Ahjond Garmestani, Dirac L. Twidwell Jr, H.E. Birgé, W. Chuang, V. M. Donovan, T. Eason, C.P. Roberts, S.M. Sundstrom, C.L. Wonkka

Department of Agronomy and Horticulture: Faculty Publications

Understanding the adaptive capacity of ecosystems to cope with change is crucial to management. However, unclear and often confusing definitions of adaptive capacity make application of this concept difficult. In this paper, we revisit definitions of adaptive capacity and operationalize the concept. We define adaptive capacity as the latent potential of an ecosystem to alter resilience in response to change. We present testable hypotheses to evaluate complementary attributes of adaptive capacity that may help further clarify the components and relevance of the concept. Adaptive sampling, inference and modeling can reduce key uncertainties incrementally over time and increase learning about adaptive …

Characterization Of The Transcriptional Divergence Between The Subspecies Of Cultivated Rice (Oryza Sativa), Malachy T. Campbell, Qian Du, Kan Li, Sandeep Sharma, Chi Zhang, Harkamal Walia

Agronomy & Horticulture -- Faculty Publications

Background: Cultivated rice consists of two subspecies, Indica and Japonica, that exhibit well-characterized differences at the morphological and genetic levels. However, the differences between these subspecies at the transcriptome level remains largely unexamined. Here, we provide a comprehensive characterization of transcriptome divergence and cis-regulatory variation within rice using transcriptome data from 91 accessions from a rice diversity panel (RDP1).

Results: The transcriptomes of the two subspecies of rice are highly divergent. Japonica have significantly lower expression and genetic diversity relative to Indica, which is likely a consequence of a population bottleneck during Japonica domestication. We leveraged high-density genotypic data and …

Factors Associated With Larval Freshwater Drum Annual Peak Density In A Nebraska Irrigation Reservoir, Brett T. Miller, Brian C. Peterson, Keith D. Koupal, Casey W. Schoenebeck

The Prairie Naturalist

Freshwater drum (Aplodinotus grunniens) represent one of the most widely distributed fish species in North America. Identifying biotic and abiotic factors that influence larval freshwater drum densities can improve understanding of early life history. Our objective was to investigate correlations between annual peak density of larval freshwater drum and combinations of five variables (chlorophyll a, growing degree days [GDD], reservoir discharge, turbidity, and cladoceran density) from a longterm (2003–2017) monitoring program in a Nebraska irrigation reservoir. Twenty-eight a priori candidate models were assessed to determine the relative support of explanatory variables associated with annual peak density of larval freshwater drum …

Captive Ring-Necked Pheasant Response To Very High Experimental Doses Of Lead, Travis J. Runia, Alex J. Solem

The Prairie Naturalist

Ingestion of spent lead pellets is a well-documented source of lead exposure in free-ranging birds, although the consequence of lead ingestion varies widely among avian guilds. Ring-necked pheasants (Phasianus colchicus) appear to be less susceptible to lead poisoning than other game birds. Our objectives were to determine survival, liver lead accumulation, and body mass change of 129 captive-raised pheasants in response to being gavage-fed 5, 10, 20, or 40 lead pellets. All pheasants survived the 21-day experiment. Liver-lead levels were positively correlated with the number of lead pellets retained and negatively correlated with beginning body mass. Change in percent body …

Evaluation Of Four Larval Fish Sampling Methods In A Large Midwestern River, Nathan J. Lederman, Anthony R. Sindt, Douglas J. Dieterman, John D. Krenz, Shannon J. Fisher

The Prairie Naturalist

Understanding limitations of larval fish capture gears is critical for developing appropriate sampling protocols and interpreting catch data. We evaluated genera richness, genera diversity, assemblage similarities, abundance indices (i.e., density or catch per unit effort [CPUE]), and sample size requirements between a surface slednet and glow-stick light traps used in 2014 and 2015 and a benthic slednet and light-emitting diode light (LED) traps used in 2015 in the Minnesota River. The surface slednet captured the greatest number of larval fish genera (15) while the LED light trap captured the fewest (1). Similarities of assemblages sampled was highest between surface and …

Comparing Native Bee Communities On Reconstructed And Remnant Prairie In Missouri, Joseph Larose, Elisabeth B. Webb, Deborah Finke

The Prairie Naturalist

The tallgrass prairie of North America is an imperiled ecosystem that has been the subject of considerable restoration effort and research in the past two decades. While native prairie plant species are purposely introduced during restoration, prairie invertebrates, including native bees (Anthophila), are not and must colonize from surrounding remnants. Prairie restorations may not support the same bee communities as remnant prairies because of habitat differences and dispersal limitations. We sampled native bees on reconstructed and remnant prairies in Missouri in the summers of 2016 and 2017 and compared the communities by evaluating species richness, diversity, and community composition. We …

Greater Prairie-Chickens And Sharp-Tailed Grouse Have Similarly High Nest Survival In The Nebraska Sandhills, Larkin Powell, Walter Schacht, Julia P. Ewald, Kathryn R. Mccollum

The Prairie Naturalist

The ranges of two native galliform species overlap in the Nebraska Sandhills, the largest contiguous grassland in North America. We monitored nests of greater prairie-chickens (Tympanuchus cupido) and sharp-tailed grouse (Tympanuchus phasianellus) in Cherry County, Nebraska, in 2015 and 2016. Our objectives were to 1) compare daily probability of nest survival between species, 2) evaluate vegetation structure at nests for potential effects on nest survival, 3) compare nest site topography between species, and 4) use a simple model of breeding season success to evaluate the potential for stable populations at our study sites. We captured and …

Dicamba Retention In Commercial Sprayers Following Triple Rinse Cleanout Procedures, And Soybean Response To Contamination Concentrations, Frances B. Browne, Xiao Li, Katilyn J. Price, Jianping Wang, Yi Wang, Greg R. Kruger, Jeff Golus, Gabrielle De Castro Macedo, Bruno C. Vieira, Tyler Sandlin

Department of Agronomy and Horticulture: Faculty Publications

The commercial launch of dicamba‐tolerant (DT) crops has resulted in increased dicamba usage and a high number of dicamba off‐target movement complaints on sensitive soybeans (Glycine max L.). Dicamba is a synthetic auxin and low dosages as 0.028 g ae ha−1 can induce injury on sensitive soybean. Tank contamination has been identified as one of the sources for unintended sensitive crop exposure. The labels of new dicamba formulations require a triple rinse cleanout procedure following applications. Cleanout efficacy might vary based on the sprayer type and procedure followed. This study was performed to quantify dicamba retention in commercial sprayers and …

Improving On Modis Mcd64a1 Burned Area Estimates In Grassland Systems: A Case Study In Kansas Flint Hills Tall Grass Prairie, Rheinhardt Scholtz, Jayson Prentice, Yao Tang, Dirac Twidwell

Department of Agronomy and Horticulture: Faculty Publications

Uncertainty in satellite-derived burned area estimates are especially high in grassland systems, which are some of the most frequently burned ecosystems in the world. In this study, we compare differences in predicted burned area estimates for a region with the highest fire activity in North America, the Flint Hills of Kansas, USA, using the moderate resolution imaging spectroradiometer (MODIS) MCD64A1 burned area product and a customization of the MODIS MCD64A1 product using a major ground-truthing effort by the Kansas Department of Health and Environment (KDHE-MODIS customization). Local-scale ground-truthing and the KDHE-MODIS product suggests MODIS burned area estimates under predicted fire …

Benchmarking The Agronomic Performance Of Biodegradable Mulches Against Polyethylene Mulch Film: A Meta‐Analysis, Mauro B.D. Tofanelli, Samuel E. Wortman

Department of Agronomy and Horticulture: Faculty Publications

Growers are interested in biodegradable alternatives to petroleum‐based polyethylene mulch film (PEM). However, many growers cite limited knowledge about biodegradable mulch films (BDMs) as a significant barrier to adoption. Agronomic field tests of BDMs are often limited temporally or spatially, and the variability of performance results relative to PEM may be contributing to this perceived knowledge gap. Our objective was to use data available in the scientific literature to provide the first quantitative performance benchmark of BDMs against PEM. We extracted data from 66 articles for meta‐analysis. Response ratios were calculated for comparison of BDMs relative to black PEM, and …

Growth And Development, From: Forages: The Science Of Grassland Agriculture, Volume Ii, Robert B. Mitchell, Daren Redfearn, Kenneth J. Moore

Department of Agronomy and Horticulture: Faculty Publications

The growth and development of forage plants is an amazing process. In some annual grasses such as cereal rye, plants can go from the late vegetative stage to fully-flowered in less than two weeks. Conversely, some perennial grasses like indiangrass can go from the vegetative stage to the elongation stage, then enter a quiescent phase for several weeks until adequate moisture is available which then moves plants into the flowering stages to complete the seed production process. Understanding the developmental morphology of forage plants is important for making good management decisions. Many such decisions involve timing the initiation or termination …

Land-Use Type As A Driver Of Large Wildfire Occurrence In The U.S. Great Plains, Victoria M. Donovan, Carissa L. Wonkka, David A. Wedin, Dirac Twidwell

Department of Agronomy and Horticulture: Faculty Publications

Wildfire activity has surged in North America’s temperate grassland biome. Like many biomes, this system has undergone drastic land-use change over the last century; however, how various land-use types contribute to wildfire patterns in grassland systems is unclear. We determine if certain land-use types have a greater propensity for large wildfire in the U.S. Great Plains and how this changes given the percentage of land covered by a given land-use type. Almost 90% of the area burned in the Great Plains occurred in woody and grassland land-use types. Although grassland comprised the greatest area burned by large wildfires, woody vegetation …

A Justification For Continued Management Of Turfgrass During Economic Contraction, James T. Brosnan, Ambika Chandra, Roch E. Gaussoin, Alec Kowalewski, Bernd Leinauer, Frank S. Rossi, Douglas J. Soldat, John C. Stier, J. Bryan Unruh

Department of Agronomy and Horticulture: Faculty Publications

A novel coronavirus, termed COVID-19, spread worldwide and become a global pandemic in 2020. Forecasts show that COVID-19 will cause substantial economic contraction affecting almost every industry. Managed turfgrass, particularly in urban settings, has many positive societal and environmental benefits. In a contracted economy, will resources be available to manage turfgrass to achieve these benefits? In this paper, we outline the benefits of managed turfgrass on golf courses, playing fields, recreational parks, and urban landscapes to assist decision makers with resource allocation in the COVID-19 era.

Training Future Agriculture Professionals In Landowner–Tenant Conservation Decision-Making, Andrea Basche, Angie Carter

Department of Agronomy and Horticulture: Faculty Publications

The landowner–tenant relationship is important to the implementation of conser- vation on agricultural lands. Women own or co-own a significant portion of U.S. farmland yet are underrepresented in conservation research. The next generation of agriculture professionals can benefit from first-hand experience in assisting women landowners and their tenants in navigating the complexities of conser- vation decision-making. This article analyzes undergraduate student perceptions of landowner–tenant relationships in conservation management through their engagement in case studies with women landowner–tenant pairs in the Western Corn Belt. Student groups were asked to complete a management improvement plan that both incorporated the agronomic and conservation …

Winter Cover Crop Root Biomass Yield In Corn And Soybean Systems, Sabrina J. Ruis, Humberto Blanco-Canqui, Katja Koehler-Cole, Paul J. Jasa, G. Slater, Roger W. Elmore, Richard B. Ferguson

Department of Agronomy and Horticulture: Faculty Publications

Cover crop (CC) roots are critical for soil ecosystem service delivery including soil stabilization, C and nutrient cycling, soil health improvement, and others. However, most CC studies only evaluate CC aboveground biomass yield, neglecting the belowground portion of the plant. The objectives of this study were to quantify the impacts of (a) CC planting (pre- and post-harvest) dates and (b) early (2–4 wk before main crop planting) and late (at main crop planting) CC termination with and without corn (Zea mays L.) residue removal on root biomass yield. We assessed the effects of CC planting or termination dates on root …

Evaluating The Impact Of Grafting On Local Tomato Production In Nebraska, Raihanah Hassim, Samuel E. Wortman, Ashley A. Thompson, Stacy A. Adams

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grafting has been successfully used in vegetable production for tomatoes, peppers, eggplants, cucumbers, and watermelon. Besides its usefulness for managing soil-borne diseases, grafting can improve nutrient uptake and yield. However, few studies have assessed the effects of grafting and soil fertility management on yield of open field-grown tomatoes in the Midwest. Therefore, the objective of this two-year research was to better document the effects of grafting heirloom and hybrid tomato cultivar onto hybrid tomato rootstocks on tomato yield and quality. The field experiments were located at the University of Nebraska Lincoln - East Campus in Lincoln, West Central Research and …

The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas E. Clemente, Paul E. Staswick, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought-prone rainfed environments. Since roots play a critical role in water uptake, root response to water limitations is an important component for enhancing wheat adaptation. In an effort to discover novel genetic sources for improving wheat adaptation, we characterized a wheat translocation line with a chromosomal segment from Agropyron elongatum, a wild relative of wheat, which unlike common wheat maintains root growth under limited-water conditions. By exploring the root transcriptome data, we found that reduced …

Rhizosphere Microbiome Of Arid Land Medicinal Plants And Extra Cellular Enzymes Contribute To Their Abundance, Abdul Latif Khan, Sajjad Asaf, Raeid M. M. Abed, Yen Ning Chai, Ahmed N. Al-Rawahi, Tupan Kumar Mohanta, Ahmed Al-Rawahi, Daniel P. Schachtman, Ahmed Al-Harrasi

Department of Agronomy and Horticulture: Faculty Publications

Revealing the unexplored rhizosphere microbiome of plants in arid environments can help in understanding their interactions between microbial communities and plants during harsh growth conditions. Here, we report the first investigation of rhizospheric fungal and bacterial communities of Adenium obesum, Aloe dhufarensis and Cleome austroarabica using next-generation sequencing approaches. A. obesum and A. dhufarensis grows in dry tropical and C. austroarabica in arid conditions of Arabian Peninsula. The results indicated the presence of 121 fungal and 3662 bacterial operational taxonomic units (OTUs) whilst microbial diversity was significantly high in the rhizosphere of A. obesum and A. dhufarensis and low …

Wheat Growth Monitoring And Yield Estimation Based On Multi-Rotor Unmanned Aerial Vehicle, Zhaopeng Fu, Jie Jang, Yang Gao, Brian Krienke, Meng Wang, Kaitai Zhong, Qiang Cao, Yongchao Tian, Yan Zhu, Weixing Cao, Xiaojun Liu

Department of Agronomy and Horticulture: Faculty Publications

Leaf area index (LAI) and leaf dry matter (LDM) are important indices of crop growth. Real-time, nondestructive monitoring of crop growth is instructive for the diagnosis of crop growth and prediction of grain yield. Unmanned aerial vehicle (UAV)-based remote sensing is widely used in precision agriculture due to its unique advantages in flexibility and resolution. This study was carried out on wheat trials treated with different nitrogen levels and seeding densities in three regions of Jiangsu Province in 2018–2019. Canopy spectral images were collected by the UAV equipped with a multi-spectral camera during key wheat growth stages. To verify the …

The State Of Sustainable Agriculture And Agroecology Research And Impacts: A Survey Of U.S. Scientists, Marcia Delonge, Tali Robbins, Andrea D. Basche, Lindsey Haynes-Maslow

Department of Agronomy and Horticulture: Faculty Publications

A growing body of research suggests that although sustainable agriculture, particularly agroecology, can address challenges such as those related to climate change, ecosystem services, food insecurity, and farmer livelihoods, the transition to such systems remains limited. To gain insight into the state of U.S. sustainable agriculture and agroecology, we developed a 28-question mixed-method survey that was administered to scientists in these fields. Respondents (N=168) represented diverse locations, institutions, and career stages. They offered varied definitions of sustainable agriculture, with 40% considering economic and social well-being to be core components. Respondents identified the amount and duration of public research …

Multi-Trait Random Regression Models Increase Genomic Prediction Accuracy For A Temporal Physiological Trait Derived From High-Throughput Phenotyping, Toshimi Baba, Mehdi Momen, Malachy T. Campbell, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

Random regression models (RRM) are used extensively for genomic inference and predic- tion of time-valued traits in animal breeding, but only recently have been used in plant sys- tems. High-throughput phenotyping (HTP) platforms provide a powerful means to collect high-dimensional phenotypes throughout the growing season for large populations. How- ever, to date, selection of an appropriate statistical genomic framework to integrate multiple temporal traits for genomic prediction in plants remains unexplored. Here, we demonstrate the utility of a multi-trait RRM (MT-RRM) for genomic prediction of daily water usage (WU) in rice (Oryza sativa) through joint modeling with shoot …

Gwas: Fast-Forwarding Gene Identification And Characterization In Temperate Cereals: Lessons From Barley – A Review, Ahmad M. Alqudah, Ahmed Sallam, P. Stephen Baenziger, Andreas Börner

Department of Agronomy and Horticulture: Faculty Publications

Understanding the genetic complexity of traits is an important objective of small grain temperate cereals yield and adaptation improvements. Bi-parental quantitative trait loci (QTL) linkage mapping is a pow- erful method to identify genetic regions that co-segregate in the trait of interest within the research pop- ulation. However, recently, association or linkage disequilibrium (LD) mapping using a genome-wide association study (GWAS) became an approach for unraveling the molecular genetic basis underlying the natural phenotypic variation. Many causative allele(s)/loci have been identified using the power of this approach which had not been detected in QTL mapping populations. In barley (Hordeum …

Soil Carbon Increased By Twice The Amount Of Biochar Carbon Applied After 6 Years: Field Evidence Of Negative Priming, Humberto Blanco-Canqui, David A. Laird, Emily A. Heaton, Samuel Rathke, Bharat Sharma Acharya

Department of Agronomy and Horticulture: Faculty Publications

Applying biochar to agricultural soils has been proposed as a means of sequester-ing carbon (C) while simultaneously enhancing soil health and agricultural sustain-ability. However, our understanding of the long-term effects of biochar and annual versus perennial cropping systems and their interactions on soil properties under field conditions is limited. We quantified changes in soil C concentration and stocks, and other soil properties 6 years after biochar applications to corn (Zea mays L.) and dedicated bioenergy crops on a Midwestern US soil. Treatments were as fol-lows: no-till continuous corn, Liberty switchgrass (Panicum virgatum L.), and low-diversity prairie grasses, 45% big bluestem …

Transforming Unmanned Aerial Vehicle (Uav) And Multispectral Sensor Into A Practical Decision Support System For Precision Nitrogen Management In Corn, Laura J. Thompson, Laila A. Puntel

Department of Agronomy and Horticulture: Faculty Publications

Determining the optimal nitrogen (N) rate in corn remains a critical issue, mainly due to unaccounted spatial (e.g., soil properties) and temporal (e.g., weather) variability. Unmanned aerial vehicles (UAVs) equipped with multispectral sensors may provide opportunities to improve N management by the timely informing of spatially variable, in-season N applications. Here, we developed a practical decision support system (DSS) to translate spatial field characteristics and normalized difference red edge (NDRE) values into an in-season N application recommendation. On-farm strip-trials were established at three sites over two years to compare farmer’s traditional N management to a split-application N management guided by …

Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt, Fatima A.M. Tenorio, Eileen L. Mclellan, Alison J. Eagle, Kenneth G. Cassman, Daryl Andersen, Marie Krausnick, Russell Oaklund, John Thorburn, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Benchmarking crop yields against nitrogen (N) input levels can help provide opportunities to improve N ferti-lizer efficiency and reduce N losses on maize in the US Corn Belt by identifying fields most likely to benefit from improved N management practices. Here, we evaluated a large producer database that includes field-level data on yield and applied N inputs from 9280 irrigated and rainfed fields over a 7-year period (2009–2015) in Nebraska (USA). A spatial framework, based on technology extrapolation domains, was used to cluster each field into spatial units with similar climate and soil type that represent 1.3 million ha of …

Prediction Strategies For Leveraging Information Of Associated Traits Under Single- And Multi-Trait Approaches In Soybeans, Reyna Persa, Arthur Bernardeli, Diego Jarquin

Department of Agronomy and Horticulture: Faculty Publications

The availability of molecular markers has revolutionized conventional ways to improve genotypes in plant and animal breeding through genome-based predictions. Several models and methods have been developed to leverage the genomic information in the prediction context to allow more efficient ways to screen and select superior genotypes. In plant breeding, usually, grain yield (yield) is the main trait to drive the selection of superior genotypes; however, in many cases, the information of associated traits is also routinely collected and it can potentially be used to enhance the selection. In this research, we considered different prediction strategies to leverage the information …

Overexpression Of Ferulate 5‑Hydroxylase Increases Syringyl Units In Sorghum Bicolor, Hannah M. Tetreault, Tammy Gries, Nathan Palmer, Deanna L. Funnell-Harris, Shirley Sato, Zhengxiang Ge, Gautam Sarath, Scott Sattler

Department of Agronomy and Horticulture: Faculty Publications

Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of lignin monomer, is derived from polymerization of coniferyl alcohol. In this study, the effects of manipulating S-lignin biosynthesis in sorghum (Sorghum bicolor) were evaluated. Overexpression of sorghum F5H (SbF5H), under the control of the CaMV 35S promoter, increased both S-lignin levels and the ratio of S/G lignin, while plant growth and development …

Biological And Cellular Functions Of The Microdomain-Associated Fwl/Cnr Protein Family In Plants, Sandra Thibivilliers, Andrew Farmer, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane microdomains/nanodomains are sub-compartments of the plasma membrane enriched in sphingolipids and characterized by their unique protein composition. They play important roles in regulating plant development and plant-microbe interactions including mutualistic symbiotic interactions. Several protein families are associated with the microdomain fraction of biological membranes such as flotillins, prohibitins, and remorins. More recently, GmFWL1, a FWL/CNR protein exclusively expressed in the soybean nodule, was functionally characterized as a new microdomain-associated protein. Interestingly, GmFWL1 is homologous to the tomato FW2-2 protein, a major regulator of tomato fruit development. In this review, we summarize the knowledge gained about the biological, cellular, and …

Investigation Of Heat-Induced Changes In The Grain Yield And Grains Metabolites, With Molecular Insights On The Candidate Genes In Barley, Mona F.A. Dawood, Yasser S. Moursi, Ahmed Amro, P. Stephen Baenziger, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Heat stress is one of the abiotic stresses that cause a significant reduction in barley yield. Climate change will increase the number of heatwaves, which will result in more deterioration in the agricultural sector. Therefore, understanding the physiological changes that occur in the plant to tolerate heat stress is very important. A collection of 60 Egyptian spring barley genotypes has been tested for heat stress under field conditions. To quantify the changes in yield-related traits and the grain-reserve parameters as indicators for heat tolerance, several traits were scored. The causative genes that regulate the variation of all traits of interest …

Soil Sample Timing, Nitrogen Fertilization, And Incubation Length Influence Anaerobic Potentially Mineralizable Nitrogen, Jason Clark, Kristen S. Veum, Fabián G. Fernández, Newell R. Kitchen, James J. Camberato, Paul Carter, Richard Ferguson, David W. Franzen, Daniel E. Kaiser, Carrie A. M. Laboski, Emerson Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Understanding the variables that affect the anaerobic potentially mineralizable N (PMNan) test should lead to a standard procedure of sample collection and incubation length, improving PMNan as a tool in corn (Zea mays L.) N management. We evaluated the effect of soil sample timing (preplant and V5 corn development stage [V5]), N fertilization (0 and 180 kg ha−1) and incubation length (7, 14, and 28 d) on PMNan (0–30 cm) across a range of soil properties and weather conditions. Soil sample timing, N fertilization, and incubation length affected PMNan differently based on soil and weather conditions. Preplant vs. V5 PMNan …