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Articles 1 - 30 of 1653

Full-Text Articles in Botany

Adopting Yield-Improving Practices To Meet Maize Demand In Sub-Saharan Africa Without Cropland Expansion, Fernando Aramburu-Merlos, Fatima A. M. Tenorio, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini May 2024

Adopting Yield-Improving Practices To Meet Maize Demand In Sub-Saharan Africa Without Cropland Expansion, Fernando Aramburu-Merlos, Fatima A. M. Tenorio, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Maize demand in Sub-Saharan Africa is expected to increase 2.3 times during the next 30 years driven by demographic and dietary changes. Over the past two decades, the area croppedwithmaize has expanded by 17million hectares in the region, with limited yield increase. Following this trend could potentially result in further maize cropland expansion and the need for imports to satisfy domestic demand. Here, we use data collected from 14,773 smallholder fields in the region to identify agronomic practices that can improve farm yield gains. We find that agronomic practices related to cultivar selection, and nutrient, pest, and crop management can …


Using Annual Forages To Replace Declining Cool-Season Grass Pasture Availability, Jonathon Matthew Jenkins May 2024

Using Annual Forages To Replace Declining Cool-Season Grass Pasture Availability, Jonathon Matthew Jenkins

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

As land area in grain crop production increases in the US Midwest, perennial pasture availability decreases but the demand increases. Matching the seasonal growth patterns of C3 and C4 plant species to grow forages that are complementary allows producers to fill production gaps in otherwise monoculture systems and possibly increase overall productivity. One way to fill the forage production gap of C3 grass dominated pastures is by producing a warm-season, annual forage in a separate field. However, with continual reductions in land availability, this may not be an option. Using two studies, our objectives were to improve …


Aquatic Transitions And Interspecific Relationships In Fissidens Mosses, Julia R. Butler May 2024

Aquatic Transitions And Interspecific Relationships In Fissidens Mosses, Julia R. Butler

Masters Theses

Embryophytes have undergone numerous re-invasions to aquatic habitats, impacting their morphological evolution. Despite the prominence of these transitions, their phylogenetic implications and morphological adaptations are just beginning to be investigated, especially as molecular methodologies become more accessible. We investigate these phenomena using the genus Fissidens, a diverse group of mosses encompassing both terrestrial and aquatic species. Our study integrates phylogenetic methods with morphological analyses to elucidate the evolutionary patterns within Fissidens and explore correlations between habitat transitions and morphological traits. Through extensive taxon sampling from herbarium specimens, DNA extraction, and sequencing, we construct robust phylogenetic trees using concatenated and …


Establishment Of Wildflower Islands To Enhance Roadside Health, Ecological Value, And Aesthetics, Jackson Ebbers May 2024

Establishment Of Wildflower Islands To Enhance Roadside Health, Ecological Value, And Aesthetics, Jackson Ebbers

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Roadsides provide an abundant opportunity to increase connectivity of fragmented landscapes with diminishing floral resources for pollinating insects. The ecological value of these sites is often overlooked as quality habitat for pollinators, particularly monarch butterflies, which have been experiencing severe declines due to loss of habitat and loss of milkweeds that provide food for their larvae. Land managers across the nation are realizing the potential of roadsides to provide high quality floral resources for the benefit of insects and other wildlife. Current wildflower seed mixes used by state transportation departments are often low diversity and may only be implemented following …


Public Horticulture: Process And Design Of The Lincoln Botanical Garden, Brad Kindler May 2024

Public Horticulture: Process And Design Of The Lincoln Botanical Garden, Brad Kindler

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Beginning in 2012, the local non-profit, The Lincoln Botanical Garden, began organizing the conceptual design and creation of a glass conservatory within a botanical garden in Lincoln, Nebraska. These proposed urban enhancements seek to fill a gap in garden amenities in the city by providing year-round access to public horticulture programming and education.

This project, Public Horticulture: Process and Design of the Lincoln Botanical Garden, documents these efforts and makes design recommendations for a themed Glacial Erratic Garden that could be constructed within the botanical garden.

Advisors: Ellen Paparozzi and Sam Wortman


Cross-Pollination: Building A Co-Taught Course To Examine Art And Sex Through The Lens Of Botany, Christopher T. Martine, Diamanda A. Zizis, Anna K. Kell May 2024

Cross-Pollination: Building A Co-Taught Course To Examine Art And Sex Through The Lens Of Botany, Christopher T. Martine, Diamanda A. Zizis, Anna K. Kell

Faculty Journal Articles

Driven by overlapping interests in plants, art, and diversity in sex expression, Anna Kell (Department of Art and Art History) and Chris Martine (Department of Biology) developed a course that integrates the perspectives of a visual artist and a botanist. Art & Sex Through the Lens of Botany seeks to impart the importance of making connections across disciplines and the value of visual literacy across academic lines. The course introduces foundational concepts in each field and encourages students to integrate and explore these different systems of knowledge and their intersections. In addition to developing fluencies related to both general botany …


Overexpression Of Msdreb1c Modulates Growth And Improves Forage Quality In Tetraploid Alfalfa (Medicago Sativa L.), Yangyang Zhang, Zhen Wang, Fan Zhang, Xue Wang, Yajing Li, Ruicai Long, Mingna Li, Xianyang Li, Quanzhen Wang, Qingchuan Yang, Junmei Kang Apr 2024

Overexpression Of Msdreb1c Modulates Growth And Improves Forage Quality In Tetraploid Alfalfa (Medicago Sativa L.), Yangyang Zhang, Zhen Wang, Fan Zhang, Xue Wang, Yajing Li, Ruicai Long, Mingna Li, Xianyang Li, Quanzhen Wang, Qingchuan Yang, Junmei Kang

Department of Agronomy and Horticulture: Faculty Publications

DREB has been reported to be involved in plant growth and response to environmental factors. However, the function of DREB in growth and development has not been elucidated in alfalfa (Medicago sativa L.), a perennial tetraploid forage cultivated worldwide. In this study, an ortholog of MtDREB1C was characterized from alfalfa and named MsDREB1C accordingly. MsDREB1C was significantly induced by abiotic stress. The transcription factor MsDREB1C resided in the nucleus and had self-transactivation activity. The MsDREB1C overexpression (OE) alfalfa displayed growth retardation under both long-day and short-day conditions, which was supported by decreased MsGA20ox and upregulated MsGA2ox in the OE …


Examination Of Greenhouse Conditions That Influence Scale Infestation Rates On Begonia, Madeline M. Cusick Apr 2024

Examination Of Greenhouse Conditions That Influence Scale Infestation Rates On Begonia, Madeline M. Cusick

Honors College Theses

Scale insects are common pests within greenhouse and agricultural settings. I quantified scale infestation on Begonia x Richmondensis, also known as the Dragon Wing begonia, within the Armstrong Campus Greenhouse from March to November of 2023. Six different treatments accounted for variations in sunlight, humidity, and temperature within the greenhouse with the goal of determining the insect’s preferred environmental conditions. Morphological comparisons were also made between host plant Dragon Wing begonia and other begonias present within the greenhouse. Analysis indicated a significant difference in scale presence on replicate plants by zone and sunlight treatments, with more plants hosting at least …


Toward A Coordinated Understanding Of Hydro-Biogeochemical Root Functions In Tropical Forests For Application In Vegetation Models, Daniela F. Cusack, Bradley O. Christoffersen, Chris M. Smith-Martin, Kelly M. Andersen, Amanda L. Cordeiro, Katrin Fleischer, S. Joseph Wright, Nathaly R. Guerrero-Ramírez, Laynara F. Lugli, Lindsay A. Mcculloch Apr 2024

Toward A Coordinated Understanding Of Hydro-Biogeochemical Root Functions In Tropical Forests For Application In Vegetation Models, Daniela F. Cusack, Bradley O. Christoffersen, Chris M. Smith-Martin, Kelly M. Andersen, Amanda L. Cordeiro, Katrin Fleischer, S. Joseph Wright, Nathaly R. Guerrero-Ramírez, Laynara F. Lugli, Lindsay A. Mcculloch

School of Integrative Biological and Chemical Sciences Faculty Publications and Presentations

Tropical forest root characteristics and resource acquisition strategies are underrepresented in vegetation and global models, hampering the prediction of forest–climate feedbacks for these carbon-rich ecosystems. Lowland tropical forests often have globally unique combinations of high taxonomic and functional biodiversity, rainfall seasonality, and strongly weathered infertile soils, giving rise to distinct patterns in root traits and functions compared with higher latitude ecosystems. We provide a roadmap for integrating recent advances in our understanding of tropical forest belowground function into vegetation models, focusing on water and nutrient acquisition. We offer comparisons of recent advances in empirical and model understanding of root characteristics …


Providing Pest Management Education For Home Gardeners In Utah, Nick Volesky, Marion Murray Apr 2024

Providing Pest Management Education For Home Gardeners In Utah, Nick Volesky, Marion Murray

Outcomes and Impact Quarterly

In January 2024, Utah State University (USU) Extension's Integrated Pest Management (IPM) program launched a three-part class series targeting Utah's home gardeners. The goal was to enhance their abilities in identifying and managing insect and plant diseases. With over 200 participants, the series notably increased the knowledge of attendees. This initiative aligns with the USU Extension IPM program's mission to promote sustainable pest management practices across Utah, evidencing its commitment to environmental stewardship and community education.


Variation In Leaf Chlorophyll Concentration In Response To Nitrogen Application Across Maize Hybrids In Contrasting Environments, Kyle M. Linders, Dipak K. Santra, James C. Schnable, Brandi Sigmon Mar 2024

Variation In Leaf Chlorophyll Concentration In Response To Nitrogen Application Across Maize Hybrids In Contrasting Environments, Kyle M. Linders, Dipak K. Santra, James C. Schnable, Brandi Sigmon

Department of Agronomy and Horticulture: Faculty Publications

Leaf chlorophyll concentration was measured for 84 publicly available maize hybrids grown under three nitrogen fertilizer treatments in two contrasting environments in Nebraska. The effect of nitrogen treatment on chlorophyll response was found to be significant (p < 0.05) for both locations. In Scottsbluff, chlorophyll concentrations increased significantly with increasing nitrogen rate, while no significant difference was found between medium and high nitrogen in Lincoln. Within equivalent nitrogen treatments, chlorophyll was more abundant in Lincoln than Scottsbluff for nearly every hybrid. Hybrid response was not consistent between environments, with approximately 11% of variance explained by genotype by environment interaction.


Toward A Coordinated Understanding Of Hydro-Biogeochemical Root Functions In Tropical Forests For Application In Vegetation Models, Daniela F. Cusack, Bradley Christoffersen, Chris M. Smith-Martin, Kelly M. Andersen, Amanda L. Cordeiro, Katrin Fleischer, S. Joseph Wright, Nathaly R. Guerrero-Ramírez, Laynara F. Lugli, Lindsay A. Mcculloch, Mareli Sanchez-Julia, Sarah A. Batterman, Caroline Dallstream, Claire Fortunel, Laura Toro, Lucia Fuchslueger, Michelle Y. Wong, Daniela Yaffar, Joshua B. Fisher, Marie Arnaud, Lee H. Dietterich, Shalom D. Addo-Danso, Oscar J. Valverde-Barrantes, Monique Weemstra, Jing Cheng Ng, Richard J. Norby Feb 2024

Toward A Coordinated Understanding Of Hydro-Biogeochemical Root Functions In Tropical Forests For Application In Vegetation Models, Daniela F. Cusack, Bradley Christoffersen, Chris M. Smith-Martin, Kelly M. Andersen, Amanda L. Cordeiro, Katrin Fleischer, S. Joseph Wright, Nathaly R. Guerrero-Ramírez, Laynara F. Lugli, Lindsay A. Mcculloch, Mareli Sanchez-Julia, Sarah A. Batterman, Caroline Dallstream, Claire Fortunel, Laura Toro, Lucia Fuchslueger, Michelle Y. Wong, Daniela Yaffar, Joshua B. Fisher, Marie Arnaud, Lee H. Dietterich, Shalom D. Addo-Danso, Oscar J. Valverde-Barrantes, Monique Weemstra, Jing Cheng Ng, Richard J. Norby

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Tropical forest root characteristics and resource acquisition strategies are underrepresented in vegetation and global models, hampering the prediction of forest–climate feedbacks for these carbon-rich ecosystems. Lowland tropical forests often have globally unique combinations of high taxonomic and functional biodiversity, rainfall seasonality, and strongly weathered infertile soils, giving rise to distinct patterns in root traits and functions compared with higher latitude ecosystems. We provide a roadmap for integrating recent advances in our understanding of tropical forest belowground function into vegetation models, focusing on water and nutrient acquisition. We offer comparisons of recent advances in empirical and model understanding of root characteristics …


U.S. Cereal Rye Winter Cover Crop Growth Database, Alexandra M. Huddell, Resham Thapa, Guillermo S. Marcillo, Lori J. Abendroth, Victoria J. Ackroyd, Shalamar D. Armstrong, Gautam Asmita, Muthukumar V. Bagavathiannan, Kipling S. Balkcom, Andrea Basche, Shawn Beam, Kevin Bradley, Lucas Pecci Canisares, Heather Darby, Adam S. Davis, Pratap Devkota, Warren A. Dick, Jeffery A. Evans, Wesley J. Everman, Tauana Ferreira De A Lmeida, Michael L. Flessner, Lisa M. Fultz, Stefan Gailans, Masoud Hashemi, Joseph Haymaker, Matthew J. Helmers, Nicholas Jordan, Thomas C. Kaspar, Quirine M. Ketterings, Eileen Kladivko, Alexandra Kravchenko, Eugene P. Law, Lauren Lazaro, Ramon G. Leon, Jeffrey Liebert, John Lindquist, Kristen Loria, Jodie M. Mcvane, Jarrod O. Miller, Michael J. Mulvaney, Nsalambi V. Nkongolo, Jason K. Norsworthy, Binaya Parajuli, Christopher Pelzer, Cara Peterson, Hanna Poffenbarger, Pratima Poudel, Mark S. Reiter, Matt Ruark, Matthew R. Ryan, Spencer Samuelson, John E. Sawyer, Sarah Seehaver, Lovreet S. Shergill, Yogendra Raj Upadhyaya, Mark Vangessel, Ashley L. Waggoner, John M. Wallace, Samantha Wells, Charles White, Bethany Wolters, Alex Woodley, Rongzhong Ye, Eric Youngerman, Brian A. Needelman, Steven B. Mirsky Feb 2024

U.S. Cereal Rye Winter Cover Crop Growth Database, Alexandra M. Huddell, Resham Thapa, Guillermo S. Marcillo, Lori J. Abendroth, Victoria J. Ackroyd, Shalamar D. Armstrong, Gautam Asmita, Muthukumar V. Bagavathiannan, Kipling S. Balkcom, Andrea Basche, Shawn Beam, Kevin Bradley, Lucas Pecci Canisares, Heather Darby, Adam S. Davis, Pratap Devkota, Warren A. Dick, Jeffery A. Evans, Wesley J. Everman, Tauana Ferreira De A Lmeida, Michael L. Flessner, Lisa M. Fultz, Stefan Gailans, Masoud Hashemi, Joseph Haymaker, Matthew J. Helmers, Nicholas Jordan, Thomas C. Kaspar, Quirine M. Ketterings, Eileen Kladivko, Alexandra Kravchenko, Eugene P. Law, Lauren Lazaro, Ramon G. Leon, Jeffrey Liebert, John Lindquist, Kristen Loria, Jodie M. Mcvane, Jarrod O. Miller, Michael J. Mulvaney, Nsalambi V. Nkongolo, Jason K. Norsworthy, Binaya Parajuli, Christopher Pelzer, Cara Peterson, Hanna Poffenbarger, Pratima Poudel, Mark S. Reiter, Matt Ruark, Matthew R. Ryan, Spencer Samuelson, John E. Sawyer, Sarah Seehaver, Lovreet S. Shergill, Yogendra Raj Upadhyaya, Mark Vangessel, Ashley L. Waggoner, John M. Wallace, Samantha Wells, Charles White, Bethany Wolters, Alex Woodley, Rongzhong Ye, Eric Youngerman, Brian A. Needelman, Steven B. Mirsky

Department of Agronomy and Horticulture: Faculty Publications

Winter cover crop performance metrics (i.e., vegetative biomass quantity and quality) affect ecosystem services provisions, but they vary widely due to differences in agronomic practices, soil properties, and climate. Cereal rye (Secale cereale) is the most common winter cover crop in the United States due to its winter hardiness, low seed cost, and high biomass production. We compiled data on cereal rye winter cover crop performance metrics, agronomic practices, and soil properties across the eastern half of the United States. The dataset includes a total of 5,695 cereal rye biomass observations across 208 site-years between 2001–2022 and encompasses …


Filling The Agronomic Data Gap Through A Minimum Data Collection Approach, Fatima A. Tenorio, Juan I. Rattalino Edreira, Juan Pablo Monzon, Fernando Aramburu-Merlos, Achim Dobermann, Armelle Gruere, Juan Martin Brihet, Sofia Gayo, Shawn Conley, Spyridon Mourtzinis, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini Jan 2024

Filling The Agronomic Data Gap Through A Minimum Data Collection Approach, Fatima A. Tenorio, Juan I. Rattalino Edreira, Juan Pablo Monzon, Fernando Aramburu-Merlos, Achim Dobermann, Armelle Gruere, Juan Martin Brihet, Sofia Gayo, Shawn Conley, Spyridon Mourtzinis, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Context: Agronomic data such as applied inputs, management practices, and crop yields are needed for assessing productivity, nutrient balances, resource use efficiency, as well as other aspects of environmental and economic performance of cropping systems. In many instances, however, these data are only available at a coarse level of aggregation or simply do not exist.

Objectives: Here we developed an approach that identifies sites for agronomic data collection for a given crop and country, seeking a balance between minimizing data collection efforts and proper representation of the main crop producing areas.

Methods: The developed approach followed a …


High Target Homology Does Not Guarantee Inhibition: Aminothiazoles Emerge As Inhibitors Of Plasmodium Falciparum, Sandra Johannsen, Robin M. Gierse, Arne KrüGer, Rachel L. Edwards, Vittoria Nanna, Anna Fontana, Di Zhu, Tiziana Masini, Lais Pessanha De Carvalho, Mael Poizat, Bart Kieftenbelt, Dana M. Hodge, Sophie University Of Nebraska-Lincoln,, Daan Bunt, Antoine Lacour, Atanaz Shams, Kamila Anna Meissner, Edmarcia Elisa De Souza, Melloney Dröge, Bernard Van Vliet, Jack Den Hartog, Michael C. Hutter, Jana Held, Audrey R. Odom John, Carsten Wrenger, Anna K.H. Hirsch Jan 2024

High Target Homology Does Not Guarantee Inhibition: Aminothiazoles Emerge As Inhibitors Of Plasmodium Falciparum, Sandra Johannsen, Robin M. Gierse, Arne KrüGer, Rachel L. Edwards, Vittoria Nanna, Anna Fontana, Di Zhu, Tiziana Masini, Lais Pessanha De Carvalho, Mael Poizat, Bart Kieftenbelt, Dana M. Hodge, Sophie University Of Nebraska-Lincoln,, Daan Bunt, Antoine Lacour, Atanaz Shams, Kamila Anna Meissner, Edmarcia Elisa De Souza, Melloney Dröge, Bernard Van Vliet, Jack Den Hartog, Michael C. Hutter, Jana Held, Audrey R. Odom John, Carsten Wrenger, Anna K.H. Hirsch

Department of Agronomy and Horticulture: Faculty Publications

In this study, we identified three novel compound classes with potent activity against Plasmodium falciparum, the most dangerous human malarial parasite. Resistance of this pathogen to known drugs is increasing, and compounds with different modes of action are urgently needed. One promising drug target is the enzyme 1-deoxy-D-xylulose-5- phosphate synthase (DXPS) of the methylerythritol 4-phosphate (MEP) pathway for which we have previously identified three active compound classes against Mycobacterium tuberculosis. The close structural similarities of the active sites of the DXPS enzymes of P. falciparum and M. tuberculosis prompted investigation of their antiparasitic action, all classes …


Environmental Biology Masters Capstone, Antonio Gonzalez-Pita Jan 2024

Environmental Biology Masters Capstone, Antonio Gonzalez-Pita

Regis University Student Publications (comprehensive collection)

Human wildlife interactions (HWI) pose a complex challenge for wildlife managers. Human encroachment into wildlife habitat and the growing number of outdoor recreationists are increasing the frequency of contact and conflict, especially in regions such as the Front Range of Colorado. Geographic information systems (GIS), which use a combination of remote sensing and environmental survey data, allow for predictive spatial analyses of where human wildlife interactions are likely to occur. I used publicly reported observations of moose to create spatial predictive maps in a species distribution model framework. Slope and elevation were shown to be the strongest predictors of HWI, …


Hop Cone Drying For The Small Grower: Temperature And Airflow Considerations, Stacy A. Adams, David M. Mabie, Michael F. Kocher, David Jones Jan 2024

Hop Cone Drying For The Small Grower: Temperature And Airflow Considerations, Stacy A. Adams, David M. Mabie, Michael F. Kocher, David Jones

Department of Agronomy and Horticulture: Faculty Publications

Small hop growers without nearby processors for cone stripping and drying must attempt to do so on their own farm. Challenges exist for self-built drying systems, including drying capacity, processing speed, airflow direction, and maintaining quality during drying. Research-based recommendations are given for optimal temperature, sizing of drying vessel, maximum cone depth, and influences associated with airflow on processing uniformity and cone quality are presented.


Characterizing New Plant Fossils With Woody Growth From The Battery Point Formation Of Quebec (Canada), Emma T. Casselman Jan 2024

Characterizing New Plant Fossils With Woody Growth From The Battery Point Formation Of Quebec (Canada), Emma T. Casselman

Cal Poly Humboldt theses and projects

The oldest woody growth has been recognized in several Early Devonian (ca. 410-395 Ma) euphyllophytes. Their taxonomic diversity is difficult to evaluate due to a lack of quantitative methods for comparing plants based on secondary xylem anatomy. In turn, this hinders understanding of their implications for the evolution of plant development. To develop metrics that quantify secondary xylem anatomy and allow for conclusive comparisons, I investigated extant taxa and Early Devonian fossil woody taxa. I developed multiple metrics for quantifying tracheid size as a function of position in the secondary xylem and tested them on a dataset of extant gymnosperms. …


Evolutionary Origins Of Secondary Growth - The Periderm Perspective: Integrating Evidence From Fossils And Living Plants, Madison A.K. Lalica Jan 2024

Evolutionary Origins Of Secondary Growth - The Periderm Perspective: Integrating Evidence From Fossils And Living Plants, Madison A.K. Lalica

Cal Poly Humboldt theses and projects

Periderm is a structural feature with roles in protection of inner plant tissues and wound healing. Knowledge of periderm occurrences in the fossil record and living lineages outside the seed plants is limited and its evolutionary origins remain poorly explored. Here, I review the known taxonomic distribution of canonical periderm (typical ontogenetic stage) and wound periderm (self-repair mechanism). To this sparse body of data I add new observations and experiments on living plant lineages and new occurrences from the fossil record. One of the latter, documented in the new early euphyllophyte species Nebuloxyla mikmaqiana, joins the oldest known periderm …


Reducing Food Scarcity: The Benefits Of Urban Farming, S.A. Claudell, Emilio Mejia Dec 2023

Reducing Food Scarcity: The Benefits Of Urban Farming, S.A. Claudell, Emilio Mejia

Journal of Nonprofit Innovation

Urban farming can enhance the lives of communities and help reduce food scarcity. This paper presents a conceptual prototype of an efficient urban farming community that can be scaled for a single apartment building or an entire community across all global geoeconomics regions, including densely populated cities and rural, developing towns and communities. When deployed in coordination with smart crop choices, local farm support, and efficient transportation then the result isn’t just sustainability, but also increasing fresh produce accessibility, optimizing nutritional value, eliminating the use of ‘forever chemicals’, reducing transportation costs, and fostering global environmental benefits.

Imagine Doris, who is …


Crop Dna Extraction With Lab-Made Magnetic Nanoparticles, Haichuan Wang, Xueqi Zhao, Li Tan, Junwei Zhu, David Hyten Dec 2023

Crop Dna Extraction With Lab-Made Magnetic Nanoparticles, Haichuan Wang, Xueqi Zhao, Li Tan, Junwei Zhu, David Hyten

Department of Agronomy and Horticulture: Faculty Publications

Molecular breeding methods, such as marker-assisted selection and genomic selection, require high-throughput and cost-effective methods for isolating genomic DNA from plants, specifically from crop tissue or seed with high polysaccharides, lipids, and proteins. A quick and inexpensive high-throughput method for isolating genomic DNA from seed and leaf tissue from multiple crops was tested with a DNA isolation method that combines CTAB extraction buffer and lab-made SA-coated magnetic nanoparticles. This method is capable of isolating quality genomic DNA from leaf tissue and seeds in less than 2 hours with fewer steps than a standard CTAB extraction method. The yield of the …


Microbiome-Enabled Genomic Selection Improves Prediction Accuracy For Nitrogen-Related Traits In Maize, Zhikai Yang, Tianjing Zhao, Hao Cheng, Jinliang Yang Dec 2023

Microbiome-Enabled Genomic Selection Improves Prediction Accuracy For Nitrogen-Related Traits In Maize, Zhikai Yang, Tianjing Zhao, Hao Cheng, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Root-associated microbiomes in the rhizosphere (rhizobiomes) are increasingly known to play an important role in nutrient acquisition, stress tolerance, and disease resistance of plants. However, it remains largely unclear to what extent these rhizobiomes contribute to trait variation for different genotypes and if their inclusion in the genomic selection protocol can enhance prediction accuracy. To address these questions, we developed a microbiome-enabled genomic selection method that incorporated host SNPs and amplicon sequence variants from plant rhizobiomes in a maize diversity panel under high and low nitrogen (N) field conditions. Our cross-validation results showed that the microbiome-enabled genomic selection model significantly …


2020-2021 Field Seasons Of Maize Gxe Project Within The Genomes To Fields Initiative, Dayane Cristina Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Alden Perkins, Dylan L. Schoemaker, Martin Costa, Kathryn J. Michel, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, James Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon Dec 2023

2020-2021 Field Seasons Of Maize Gxe Project Within The Genomes To Fields Initiative, Dayane Cristina Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Alden Perkins, Dylan L. Schoemaker, Martin Costa, Kathryn J. Michel, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, James Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives: This release note describes the Maize GxE project datasets within the Genomes to Fields (G2F) Initiative. The Maize GxE project aims to understand genotype by environment (GxE) interactions and use the information collected to improve resource allocation efficiency and increase genotype predictability and stability, particularly in scenarios of variable environmental patterns. Hybrids and inbreds are evaluated across multiple environments and phenotypic, genotypic, environmental, and metadata information are made publicly available. Data description: The datasets include phenotypic data of the hybrids and inbreds evaluated in 30 locations across the US and one location in Germany in 2020 and 2021, soil …


Vulnerabilities Of Greater Prairie Chicken And Tier 1 At-Risk Species In Nebraska Caused By Grassland Transition To Woody Dominance, Robert Peterson Dec 2023

Vulnerabilities Of Greater Prairie Chicken And Tier 1 At-Risk Species In Nebraska Caused By Grassland Transition To Woody Dominance, Robert Peterson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Woody plant encroachment is a global threat and has been transitioning grasslands to woody dominance at a biome scale. This threat is present in the Great Plains grassland biome which is currently experiencing grassland biome collapse as the alternative woody biome advances northwest. Nebraska, which contains the most intact temperate grassland in the world, is currently at the front lines of this large-scale transition making this state’s management decisions vital for the remaining grasslands and the species which rely on these ecosystems. In this study, we assess the vulnerability of Greater Prairie Chicken and Tier 1 at-risk species in Nebraska …


Do Organic Farming Practices Improve Soil Physical Properties?, Humberto Blanco-Canqui, Sabrina J. Ruis, Charles A. Francis Nov 2023

Do Organic Farming Practices Improve Soil Physical Properties?, Humberto Blanco-Canqui, Sabrina J. Ruis, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Organic farming (OF) is a reemerging system that could address food security and adverse environmental footprints of conventional farming (CF). However, how OF affects the soil physical environment, an essential pillar for soil ecosystem service delivery, is not well understood. This paper (1) reviews published global literature up to 13 July 2023 regarding the impacts of OF on soil physical properties compared with CF and (2) underlines research needs. Literature indicates OF improves some soil physical properties relative to CF although studies on some properties were few. Specifically, OF increased wet aggregate stability, saturated hydraulic conductivity, and plant available water …


Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman Nov 2023

Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We …


Does Selection For Seedling Tiller Number In Perennial Biomass Feedstocks Translate To Yield And Quality Improvements In Mature Swards?, Robert B. Mitchell, Kenneth P. Vogel, Susan Tunnell, James Stubbendieck Nov 2023

Does Selection For Seedling Tiller Number In Perennial Biomass Feedstocks Translate To Yield And Quality Improvements In Mature Swards?, Robert B. Mitchell, Kenneth P. Vogel, Susan Tunnell, James Stubbendieck

Department of Agronomy and Horticulture: Faculty Publications

Breeding for seedling traits in herbaceous perennial biomass feedstocks that translate into increased biomass yield or quality in established swards could accelerate the development of perennial grass cultivars for bioenergy or forage. In previously reported research, breeding for single large tillers (ST) or multiple tillers (MT, ≥3) six weeks after planting for two generations in big bluestem (Andropogon gerardii Vitman) and switchgrass (Panicum virgatum L.) base populations produced ST and MT populations that differed significantly for seedling and mature plant traits including biomass yield in spaced planted nurseries. Our objective was to evaluate these ST and MT populations in sward …


A Multi-Model Approach To Understanding Growth, Reproduction, And Life History Strategies In Bromeliads, Erin N. Bodine Nov 2023

A Multi-Model Approach To Understanding Growth, Reproduction, And Life History Strategies In Bromeliads, Erin N. Bodine

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.


Effects Of Fertigation Versus Palletized Fertilizer On Height And Caliper Growth In Oaks, Zachary M. Davis Oct 2023

Effects Of Fertigation Versus Palletized Fertilizer On Height And Caliper Growth In Oaks, Zachary M. Davis

LSU Master's Theses

The Covid-19 pandemic and ensuing lockdowns led to an increase in revenue and production for green industry products in early 2020. In Study 1. an evaluation of fertigation (liquid feed, quick release) and controlled release fertilizers (CRFs, slow release) were applied to container-grown (7 gallon) live oak (Quercus virginiana) and Nutall oak (Quercus nuttallii) trees (100% Liquid feed, 0% CRF, 67% Liquid feed, 33% CRF, 33% Liquid feed, 67% CRF, 100% Liquid feed, 0% CRF). Live oak trees fertilized with CRFs had increased stem calipers (>30%). Nuttall stem caliper and height were significantly increased by …


Genetic Analysis Of Basal Stalk Rot Resistance Introgressed From Wild Helianthus Petiolaris Into Cultivated Sunflower (Helianthus Annuus L.) Using An Advanced Backcross Population, Zahirul I. Talukder, William Underwood, Christopher G. Misar, Xuehui Li, Gerald J. Seiler, Xiwen Cai, Lili Qi Oct 2023

Genetic Analysis Of Basal Stalk Rot Resistance Introgressed From Wild Helianthus Petiolaris Into Cultivated Sunflower (Helianthus Annuus L.) Using An Advanced Backcross Population, Zahirul I. Talukder, William Underwood, Christopher G. Misar, Xuehui Li, Gerald J. Seiler, Xiwen Cai, Lili Qi

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sclerotinia sclerotiorum is a serious pathogen causing severe basal stalk rot (BSR) disease on cultivated sunflower (Helianthus annuus L.) that leads to significant yield losses due to insufficient resistance. The wild annual sunflower species H. petiolaris, commonly known as prairie sunflower is known for its resistance against this pathogen. Sunflower resistance to BSR is quantitative and determined by many genes with small effects on the resistance phenotype. The objective of this study was to identify loci governing BSR resistance derived from H. petiolaris using a quantitative trait loci (QTL) mapping approach.

Methods: BSR …