Agronomy and Crop Sciences Commons^™

Next Generation Dna Sequencing Technology Delivers Valuable Genetic Markers For The Genomic Orphan Legume Species, Bituminaria Bituminosa, M Pazos-Navarro, Mercedes Dabauza, Enrique Correal, Kelly Hanson, Natasha L. Teakle, Daniel Real, Matthew Nelson

Grain and Other Field Crops Research Articles

Background
Bituminaria bituminosa is a perennial legume species from the Canary Islands and Mediterranean region that has potential as a drought-tolerant pasture species and as a source of pharmaceutical compounds. Three botanical varieties have previously been identified in this species: albomarginata, bituminosa and crassiuscula. B. bituminosa can be considered a genomic 'orphan' species with very few genomic resources available. New DNA sequencing technologies provide an opportunity to develop high quality molecular markers for such orphan species.

Results
432,306 mRNA molecules were sampled from a leaf transcriptome of a single B. bituminosa plant using Roche 454 pyrosequencing, resulting in an …

Phenological Development In Two-Row Spring Barley When Grown In A Long-Day (Alberta, Canada) And A Short-Day (Western Australia, Australia) Environment, Blakely Paynter, P E. Juskiw, J H. Helm

Grain and Other Field Crops Research Articles

Phenological development in eight cultivars of two-row, spring barley (Hordeum vulgare L.) was examined when sown at two dates of seeding in two diverse environments. These environments were a short-day environment at Northam, Western Australia, Australia, in 1997 and a long-day environment at Lacombe, Alberta, Canada, in 1998. The Australian and Canadian barley cultivars used had different combinations of basic vegetative phase and daylength sensitivity. Barley grown at Lacombe reached each stage of phenological development in less time than at Northam. Most noticeable was a shorter duration of the period between seedling emergence to double ridge and between double ridge …

Evaluation Of Perennial Forage Legumes And Herbs In Six Mediterranean Environments, Daniel Real, Guangdi D. Li, Steve Clark, Tony Albertsen, Richard C. Hayes, Matt D. Denton, Mario F. D'Antuono, B S. Dear

Grain and Other Field Crops Research Articles

There is an absence of drought tolerant herbaceous perennial forage legume and herb options other than lucerne (Medicago sativa L.) for environments with Mediterranean-like climates common in extensive areas of Southern Australia, the Mediterranean basin, and Chile. Therefore, a collection of 174 forage perennial legume and herb entries from 103 species and 32 genera was evaluated for adaptation in a diverse range of Mediterranean climatic environments in Southern Australia. The seasonal rainfall distribution varied from moderately to highly winter dominant with long term average annual rainfall ranging from 318 to 655 mm. The entries were rated for productivity and …

Selecting Improved Lotus Nodulating Rhizobia To Expedite The Development Of New Forage Species, John Gregory Howieson, Ross Andrew Ballard, Ronald J. Yates, Nigel Charman

Grain and Other Field Crops Research Articles

Aims

In the past decades the increasing focus by Australian pasture development programs on the genus Lotus has seen the evaluation of many species previously untested in Australia. In field trials, nodulation failure was commonplace. This work was undertaken to select effective symbionts for Lotus to ensure further agronomic evaluation of the genus was not compromised. The symbiotic needs of Lotus ornithopodioides were a particular focus of the studies.

Methods

Glasshouse experiments were undertaken to evaluate symbiotic relationships between 15 Lotus spp and 23 strains of nodulating Mesorhizobium loti. This was followed by evaluation of elite rhizobial strains for …

Complete Genome Sequence Of Rhizobium Leguminosarum Bv. Trifolii Strain Wsm1325, An Effective Microsymbiont Of Annual Mediterranean Clovers, Wayne Reeve, Graham William O'Hara, Patrick Chain, Julie Kaye Ardley, Lambert Brau, Kemanthi Nandesena, Ravi P. Tiwari, Alex Copeland, Matt Nolan, Cliff Han, Thomas Brettin, Miriam Land, Galina Ovchinikova, Natalia Ivanova, Konstantinos Mavromatis, Victor Markowitz, Nikos Kyrpides, Vanessa Melino, Matt D. Denton, Ronald J. Yates, John Howieson

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv trifolii is a soil-inhabiting bacterium that has the capacity to be an effective nitrogen fixing microsymbiont of a diverse range of annual Trifolium (clover) species. Strain WSM1325 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from root nodules collected in 1993 from the Greek Island of Serifos. WSM1325 is produced commercially in Australia as an inoculant for a broad range of annual clovers of Mediterranean origin due to its superior attributes of saprophytic competence, nitrogen fixation and acid-tolerance. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This …

Leaf Development In Two-Row Spring Barley Under Long-Day And Short-Day Field Conditions, Blakely Paynter, P E. Juskiw, J H. Helm

Grain and Other Field Crops Research Articles

To gain an understanding of the adaptation of Australian and Canadian barley (Hordeum vulgare L.) cultivars to the environments of western Canada and Western Australia, phyllochron and number of leaves on the mainstem in eight cultivars of two-row, spring barley were examined when sown at two dates in two locations. The locations were a short-day environment at Northam, Western Australia, Australia in 1997 and a long-day environment at Lacombe, Alberta, Canada in 1998. At each location highly significant relationships between leaf number on the mainstem and thermal time were found (r2 > 0.94). Using linear estimates, the phyllochron of barley under …

Comparison Of The Phosphate Requirements Of Burr Medic And Yellow Serradella With That Of Wheat In The Low Rainfall Wheatbelt Of Western Australia, Blakely Paynter, Mike D. Bolland

Grain and Other Field Crops Research Articles

The responses of burr medic (Medicago polymorpha) and yellow serradella (Ornithopus compressus) to topdressed phosphate were compared with the response of wheat (Triticum aestivum) to drilled phosphate on a range of soils in the low rainfall wheatbelt of Western Australia. Species were compared firstly on the amount of applied phosphorus (P) required for 90% maximum yield at 3 harvests, and secondly on the ratio of their curvature coefficients from the Mitscherlich relationship between absolute yield and P applied. On 7 marginally acidic, medium-textured soils, the comparative requirements of burr medic (cvv. Serena or Santiago) and wheat (cv. Gutha) for applied …

Comparison Of The Phosphate Requirements Of Burr Medic And Yellow Serradella With Subterranean Clover In The Low Rainfall Wheatbelt Of Western Australia, Blakely Paynter

Grain and Other Field Crops Research Articles

Burr medic (Medicago polymorpha) and yellow serradella (Ornithopus compressus) were compared with subterranean clover (Trifolium subterraneum) in their response to freshly topdressed phosphate in the low rainfall wheatbelt of Western Australia. Species were compared on the amount of applied phosphorus (P) required for 90% maximum yield and the ratio of their curvature coefficients from the Mitscherlich relationship between P applied and absolute yield. On marginally acidic, medium-textured soils, burr medic had a higher external shoot requirement for applied P than subterranean clover. Relative differences between the species were affected by season, initial concentration of bicarbonate-extractable P in the soil (0-10 …

Propagation Of Yellow Bells (Geleznowia Verrucosa Turcz., Rutaceae) From Seed, Blakely Paynter, Kingsley Dixon

Grain and Other Field Crops Research Articles

Geleznowia verrucosa Turcz. (Rutaceae) is a commercially important, bush-picked wildflower that has no known commercial means of propagation. This study examined factors influencing seed dormancy and likely to restrict germination of the species. Germination of entire, untreated seed was low (less than 2%), but removal of a small portion of the testa at or near the radicle end of the seed improved germination. This response was affected by the temperature under which the seed germinated, but was unaffected by exposure of the seed to light while germinating. The percentage germination of nicked seeds 52 days after sowing increased from 8% …

Comparative Phosphate Requirements Of Yellow Serradella (Ornithopus Compressus) Burr Medic (Medicago Polymorpha Var. Brevispina) And Subterranean Clover (Trifolium Subterraneum), Blakely Paynter

Grain and Other Field Crops Research Articles

The phosphate (P) requirements of burr medic (Medicago polymorpha) on marginally acidic, medium-textured soils and yellow serradella (Ornithopus compressus) on acidic, light-textured soils in the low rainfall (< 400 mm) wheatbelt of Western Australia are not known. The hypothesis that yellow serradella, subterranean clover (Trifolium suhrerraneum) and burr medic have the same external requirement for applied P was tested in a glasshouse trial on 2 P-deficient soils. Species were compared on an equal total seed weight and similar maturity length basis. It was found that yellow serradella required less soil applied P to achieve 90% maximum shoot production or total plant (shoots + roots) growth and had a greater curvature co-efficient from fitted Mitscherlich functions than either subterranean clover or burr medic. Burr medic was the most responsive to applied P and had the largest external requirement for applied P. These differences in external P requirements were related to differences in ability of the 3 legumes to absorb P and to transport it to their shoots and were not due to differences in internal efficiency of the shoots. Yellow serradella was able to take up more P (total plant P content) per g roots than subterranean clover, and subterranean clover more than burr medic. The same relationship applied to the translocation of absorbed P from roots to shoots.

Increasing Phosphorus Concentration In Lupin Seed Increases Grain Yield On Phosphorus Deficient Soil, Mike D. Bolland, Blakely Paynter, Mike Baker

Grain and Other Field Crops Research Articles

In a field experiment on a phosphorus (P) deficient soil in south-western Australia, lupin seed ( Lupinus angustifolius cv. Danja) of the same size (157 mg/seed) but with 2 different phosphorus (P) concentrations in the seed (2.0 and 2.8 g P/kg) was sown with 4 levels of superphosphate (5, 20, 40 and 60 kg P/ha) drilled with the seed in May 1988 to examine the effect of seed P concentration on subsequent dry matter (DM) and grain yields. Increasing the amount of superphosphate applied from 5 to 60 kg P/ha almost doubled yields. In addition, lupins grown from seed containing …