Agronomy and Crop Sciences Commons^™

Quantitative Trait Loci Mapping For Vigour And Survival Traits Of Barley Seedlings After Germinating Under Salinity Stress, Edward Mwando, Tefera Angessa, Yong Han, Gaofeng Zhou, Chengdao Li

Grain and Other Field Crops Research Articles

Seed germination and seedling establishment are the most critical stages in the barley (Hordeum vulgare ssp. vulgare L.) life cycle that contribute substantially to grain yield. These two phases are exposed to several forms of environmental stresses such as salinity due to high level of salt accumulation in the soil rhizosphere where seed germination takes place and seedlings emerge from. Previously, we have reported genotypic variability and independent QTLs associated with salinity tolerance at seedling and germination stages. However, genotypic studies on revival of a seedling germinating under salinity stress are yet to close the lack of information between germination …

Short And Long Term Effects Of Lime And Gypsum Applications On Acid Soils In A Water-Limited Environment: 2. Soil Chemical Properties, Geoff Anderson, Shahab Pathan, James Easton, David Hall, Rajesh Sharma

Grain and Other Field Crops Research Articles

Soil acidity or aluminum (Al) toxicity is a major limitation to crop production. In this paper, we examine the effects of surface-applied lime and gypsum on soil profile chemical properties that affect Al toxicity in short-term (1 year), medium-term (2 years and 8 months) and long-term (10 years) experiments. Sulfate applied to the soil surface as gypsum was leached rapidly to a depth of 40 cm in the short-term despite relatively low amounts (279 mm) of rainfall. In the medium and long-term experiments, 28–54% of the sulfate applied as gypsum was retained in the 0–50 cm soil layer due to …

Soil Management Systems To Overcome Multiple Constraints For Dryland Crops On Deep Sands In A Water Limited Environment On The South Coast Of Western Australia, David J. Hall, Stephen L. Davies, Richard W. Bell, Tom Edwards

Grain and Other Field Crops Research Articles

Deep sands on the south coast sandplain of Western Australia (WA) have multiple soil constraints including water repellence, high soil strength, low nutrient levels and subsoil acidity. The aim of the study was to test contrasting methods of managing water repellence and to assess their impacts on one or more soil constraints to crop production. These methods included seeding tyne design (knife point, winged points, paired row), soil wetting agent addition, strategic inversion tillage (rotary spading, mouldboard ploughing to 0.35 m) and clay-rich subsoil addition (170 t ha−1 with incorporation by spading to 0.20 or 0.35 m). Limesand (2 t …

Effect Of Varietal Resistance On The Yield Loss Function Of Wheat To Nodorum Blotch, Manisha Shankar, Karyn Reeves, Jason Bradley, Ryan Varischetti, Robert Loughman

Grain and Other Field Crops Research Articles

Cultivar disease resistance ratings to nodorum blotch (caused by Parastagonospora nodorum) provide critical information to growers to assist with disease management. However, less has been done in terms of yield losses from diseases under different cultivar resistance scenarios. The purpose of this study was to characterize yield and quality loss responses to wheat nodorum blotch as influenced by variety partial resistance, epidemic intensity, and environment. The trial series includes four site/years in Western Australia. Five varieties, Magenta, Calingiri, Trojan, Janz, and Scout were used for all four trials. These varieties represented resistant/susceptible categories MRMS, MS, MSS, S, and SVS, …

Slow Movement Of Alkali From Surface-Applied Lime Warrants The Introduction Of Strategic Tillage For Rapid Amelioration Of Subsurface Acidity In South-Western Australia, Gaus Azam, Chris Gazey

Grain and Other Field Crops Research Articles

Conventional surface-application of agricultural lime takes many years to increase pH deeper in the soil profile, which is a barrier to increased adoption of liming. We conducted a series of experiments to measure the rate of vertical movement of alkali and identify the factors that determine this movement into the subsurface, to evaluate the feasibility of ameliorating acidic subsurface soil using residual (undissolved) lime (CaCO3) at Wongan Hills (30.85°S, 116.74°E) and Merredin (31.48°S, 118.21°E) and to test whether deep tillage and lime incorporation can significantly speed up the amelioration of subsurface soil acidity at Kalannie (30.42°S, 117.29°E). Multiple applications of …

Short And Long Term Effects Of Lime And Gypsum Applications On Acid Soils In A Water-Limited Environment: 1. Grain Yield Response And Nutrient Concentration, Geoff Anderson, Shahab Pathan, James Easton, David Hall, Rajesh Sharma

Grain and Other Field Crops Research Articles

Surface (0–10 cm) and subsoil (soil layers below 10 cm) acidity and resulting aluminum (Al) toxicity reduce crop grain yields. In South Western Australia (SWA), these constraints affect 14.2 million hectares or 53% of the agricultural area. Both lime (L, CaCO3) and gypsum (G, CaSO4) application can decrease the toxic effect of Al, leading to an increase in crop grain yields. Within the region, it is unclear if G alone or the combined use of L and G has a role in alleviating soil acidity in SWA, due to low sulfate S (SO4–S) sorption properties of the soil. We present …

The First Genetic Map For A Psoraleoid Legume (Bituminaria Bituminosa) Reveals Highly Conserved Synteny With Phaseoloid Legumes, Matthew N. Nelson, Jafar S. Jabbari, Rust Turakulov, Aneeta Pradhan, Maria Pazos-Navarro, Jacob S. Stai, Steven B. Cannon, D. Real

Grain and Other Field Crops Research Articles

We present the first genetic map of tedera (Bituminaria bituminosa (L.) C.H. Stirton), a drought-tolerant forage legume from the Canary Islands with useful pharmaceutical properties. It is also the first genetic map for any species in the tribe Psoraleeae (Fabaceae). The map comprises 2042 genotyping-by-sequencing (GBS) markers distributed across 10 linkage groups, consistent with the haploid chromosome count for this species (n = 10). Sequence tags from the markers were used to find homologous matches in the genome sequences of the closely related species in the Phaseoleae tribe: soybean, common bean, and cowpea. No tedera linkage groups align in their …

Noodles Made From High Amylose Wheat Flour Attenuate Postprandial Glycaemia In Healthy Adults, Kim Ang, Carla Bourgy, Haelee Fenton, Ahmed Regina, Marcus Newberry, Dean A. Diepeveen, Domenico Lafiandra, Sara Grafenauer, Wendy Hunt, Vicky Solah

Grain and Other Field Crops Research Articles

Previous research has not considered the effect of high amylose wheat noodles on postprandial glycaemia. The aim of the study is to investigate the effect of consumption of high amylose noodles on postprandial glycaemia over 2-h periods by monitoring changes in blood glucose concentration and calculating the total area under the blood glucose concentration curve. Twelve healthy young adults were recruited to a repeated measure randomised, single-blinded crossover trial to compare the effect of consuming noodles (180 g) containing 15%, 20% and 45% amylose on postprandial glycaemia. Fasting blood glucose concentrations were taken via finger-prick blood samples. Postprandial blood glucose …

Tactical Crop Management For Improved Productivity In Winter-Dominant Rainfall Regions: A Review, W K. Anderson, Ross Brennan, Kithsiri W. Jayasena, Svetlana Micic, John H. Moore, T Nordblom

Grain and Other Field Crops Research Articles

This study reviews published information on the tactical management decisions needed to maximise economic grain yield in winter-dominant rainfall regions of the Mediterranean type. Tactical decisions are defined as those relating to the period from immediately before sowing to harvest. Tactical management is the principal means by which farmers respond to changing environmental and short-term economic conditions as the season progresses. The review considers published evidence that underpins these decisions and relates to cereal crops (wheat, barley and oats), pulse crops (field pea, faba bean, chickpea and narrow-leaved lupin) and canola.

The criteria used to guide management decisions during the …

Environmental Adaptation Of Leucaena In Western Australia – Challenges And Opportunities, Clinton K. Revell Dr, Geoff A. Moore Mr, Daniel Real, Sam Crouch

Grain and Other Field Crops Research Articles

There is considerable interest from Western Australian (WA) pastoralists on the potential role of leucaena(Leucaena leucocephala) in northern WA, where the potential area for dryland production of species of the genus Leucaenais high. Although it is highly regarded for animal production in other countries and in Queensland, leucaena is a contentious species since its status as an environmental weed currently precludes it from use on pastoral leases in the Kimberley and Pilbara regions of WA. Development of sterile/seedless forms would overcome risks of spread of the species as a weed. The key environmental constraints to growth of leucaena are likely …

Hybridisation-Based Target Enrichment Of Phenology Genes To Dissect The Genetic Basis Of Yield And Adaptation In Barley, Camilla Beate Hill, Tefera Angessa, Lee-Anne Mcfawn, Debbie Wong, Josquin Tibbits, Xiao-Qi Zhang, Kerrie Forrest, David Moody, Paul Telfer, Sharon Westcott, Dean A. Diepeveen, Yanhao Xu, Cong Tan, Matthew Hayden, Chengdao Li

Grain and Other Field Crops Research Articles

Barley (Hordeum vulgare L.) is a major cereal grain widely used for livestock feed, brewing malts and human food. Grain yield is the most important breeding target for genetic improvement and largely depends on optimal timing of flowering. Little is known about the allelic diversity of genes that underlie flowering time in domesticated barley, the genetic changes that have occurred during breeding, and their impact on yield and adaptation. Here, we report a comprehensive genomic assessment of a worldwide collection of 895 barley accessions based on the targeted resequencing of phenology genes. A versatile target-capture method was used to …

Nam Gene Allelic Composition And Its Relation To Grain-Filling Duration And Nitrogen Utilisation Efficiency Of Australian Wheat, Zaid Alhabbar, Rongchang Yang, Angela Juhasz, Xin Hu, Maoyun She, Masood Anwar, Nigarin Sultana, Dean Diepeveen, Wujun Ma, Shahidul Islam

Grain and Other Field Crops Research Articles

Optimising nitrogen fertiliser management in combination with using high nitrogen efficient wheat cultivars is the most effective strategy to maximise productivity in a cost-efficient manner. The present study was designed to investigate the associations between nitrogen utilisation efficiency (NUtE) and the allelic composition of the NAM genes in Australian wheat cultivars. As results, the non-functional NAM-B1 allele was more responsive to the nitrogen levels and increased NUtE significantly, leading to a higher grain yield but reduced grain protein content. Nitrogen application at different developmental stages (mid-tillering, booting, and flowering) did not show significant differences in grain yield and protein content. …

Wheat Grain Protein Accumulation And Polymerization Mechanisms Driven By Nitrogen Fertilization, Zitong Yu, Shahidul Islam, Maoyun She, Dean Diepeveen, Yujuan Zhang, Guixiang Tang, Jingjuan Zhang, Angela Juhasz, Rongchang Yang, Wujun Ma

Grain and Other Field Crops Research Articles

In wheat (Triticum aestivum) grain yield and grain protein content are negatively correlated, making the simultaneous increase of the two traits challenging. Apart from genetic approaches, modification of nitrogen fertilization offers a feasible option to achieve this aim. In this study, a range of traits related to nitrogen-use efficiency in six Australian bread wheat varieties were investigated under different nitrogen treatments using 3-year multisite field trials. Changes in the individual storage protein composition were detected by high-performance liquid chromatography. Our results indicated that wheat grain yield and grain protein content reacted similarly to nitrogen availability, with grain yield …

Impact Of Mid-Season Sulphur Deficiency On Wheat Nitrogen Metabolism And Biosynthesis Of Grain Protein, Zitong Yu, Angela Juhasz, Shahidul Islam, Dean Diepeveen, Jingjuan Zhang, Penghao Wang, Wujun Ma

Grain and Other Field Crops Research Articles

Wheat (Triticum aestivum) quality is mainly determined by grain storage protein compositions. Sulphur availability is essential for the biosynthesis of the main wheat storage proteins. In this study, the impact of different sulphur fertilizer regimes on a range of agronomically important traits and associated gene networks was studied. High-performance liquid chromatography was used to analyse the protein compositions of grains grown under four different sulphur treatments. Results revealed that sulphur supplementation had a significant effect on grain yield, harvest index, and storage protein compositions. Consequently, two comparative sulphur fertilizer treatments (0 and 30 kg ha−1 sulphur, with 50 …

Early Growth Stages Salinity Stress Tolerance In Cm72 X Gairdner Doubled Haploid Barley Population, Tefera T. Angessa, Xiao-Qi Zhang, Gaofeng Zhou, Sue Broughton, Wenying Zhang, Chengdao Li

Grain and Other Field Crops Research Articles

A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H–6H each …

Zernike Moments And Genetic Algorithm: Tutorial And Application, Oluleye Babatunde, Leisa Armstrong, Jinsong Leng, Dean Diepeveen

Grain and Other Field Crops Research Articles

Aims/ objectives: To demontrate effectiveness of Zernike Moments for Image Classification. Zernike moment(ZM) is an excellent region-based moment which has attracted the attentions of many image processing researchers since its first application to image analysis. Many papers have been published on several works done on ZM but no single paper ever give a detailed information of how the computation of ZM is done from the time the image is captured to the computation of ZM. This work showed how to effectively apply ZM on RGB images. We have demonstrated the effectiveness of Zernike moment in image classification system. A neuro-genetic …

A Neuronal Classification System For Plant Leaves Using Genetic Image Segmentation, Oluleye Babatunde, Leisa Armstrong, Dean Diepeveen, Jinsong Leng

Grain and Other Field Crops Research Articles

This paper demonstrates the use of radial basis networks (RBF), cellular neural networks (CNN)and genetic algorithm (GA) for automatic classification of plant leaves. A genetic neuronal system herein attempted to solve some of the inherent challenges facing current software being employed for plant leaf classification. The image segmentation module in this work was genetically optimized to bring salient features in the images of plants leaves used in this work. The combination of GA-based CNN with RBF in this work proved more efficient than the existing systems that use conventional edge operators such as Canny, LoG, Prewitt, and Sobel operators. The …

Potential Break Crops For Managing Root-Knot Nematode, Carla Wilkinson, Stuart Vincent, Sarah J. Collins Dr

Grain and Other Field Crops Research Articles

Western Australian growers may be unintentionally increasing root-knot nematode, Meloidogyne fallax (False Columbia root-knot nematode) populations by using susceptible break crops between cash crops.

A Genetic Algorithm-Based Feature Selection, Oluleye Babatunde, Leisa Armstrong, Jinsong Leng, Dean Diepeveen

Grain and Other Field Crops Research Articles

This article details the exploration and application of Genetic Algorithm (GA) for feature selection. Particularly a binary GA was used for dimensionality reduction to enhance the performance of the concerned classifiers. In this work, hundred (100) features were extracted from set of images found in the Flavia dataset (a publicly available dataset). The extracted features are Zernike Moments (ZM), Fourier Descriptors (FD), Lengendre Moments (LM), Hu 7 Moments (Hu7M), Texture Properties (TP) and Geometrical Properties (GP). The main contributions of this article are (1) detailed documentation of the GA Toolbox in MATLAB and (2) the development of a GA-based feature …

Genome Sequence Of Rhizobium Leguminosarum Bv Trifolii Strain Wsm1689, The Microsymbiont Of The One Flowered Clover Trifolium Uniflorum, Jason Terpolilli, Tian Rui, Ronald J. Yates, John Gregory Howieson, Philip Poole, Christine Munk, Roxanne Tapia, Cliff Han, Victor Markowitz, Reddy Tatiparthi, Konstantinos Mavromatis, Natalia Ivanova, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides, Wayne Reeve

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii is a soil-inhabiting bacterium that has the capacity to be an effective N2-fixing microsymbiont of Trifolium (clover) species. R. leguminosarum bv. trifolii strain WSM1689 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Trifolium uniflorum collected on the edge of a valley 6 km from Eggares on the Greek Island of Naxos. Although WSM1689 is capable of highly effective N2-fixation with T. uniflorum, it is either unable to nodulate or unable to fix N2 with a wide range of both perennial and annual clovers originating from Europe, North America …

Genome Sequence Of The Listia Angolensis Microsymbiont Microvirga Lotononidis Strain Wsm3557ᵀ, Wayne Reeve, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Jason Terpolilli, Vanessa Melino, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Xiaojing Zhang, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Ernest Szeto, Natalia Ivanova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Microvirga lotononidis is a recently described species of root-nodule bacteria that is an effective nitrogen- (N2) fixing microsymbiont of the symbiotically specific African legume Listia angolensis (Welw. ex Bak.) B.-E. van Wyk & Boatwr. M. lotononidis possesses several properties that are unusual in root-nodule bacteria, including pigmentation and the ability to grow at temperatures of up to 45°C. Strain WSM3557T is an aerobic, motile, Gram-negative, non-spore-forming rod isolated from a L. angolensis root nodule collected in Chipata, Zambia in 1963. This is the first report of a complete genome sequence for the genus Microvirga. Here we describe the features …

Genome Sequence Of The Ornithopus/Lupinus-Nodulating Bradyrhizobium Sp. Strain Wsm471, Wayne Reeve, Sofie E. De Meyer, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Ravi P. Tiwari, John Gregory Howieson, Ronald J. Yates, Graham William O'Hara, Mohamed Ninawi, Megan Lu, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Natalia Ivanova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Bradyrhizobium sp. strain WSM471 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen- (N2) fixing root nodule formed on the annual legume Ornithopus pinnatus (Miller) Druce growing at Oyster Harbour, Albany district, Western Australia in 1982. This strain is in commercial production as an inoculant for Lupinus and Ornithopus. Here we describe the features of Bradyrhizobium sp. strain WSM471, together with genome sequence information and annotation. The 7,784,016 bp high-quality-draft genome is arranged in 1 scaffold of 2 contigs, contains 7,372 protein-coding genes and 58 RNA-only encoding genes, and is one of 20 rhizobial …

Complete Genome Sequence Of Mesorhizobium Australicum Type Strain (Wsm2073t), Wayne Reeve, Kemanthi G. Nandasena, Ronald J. Yates, Ravi P. Tiwari, Graham William O'Hara, Mohamed Ninawi, Wei Gu, Lynne Goodwin, Chris Detter, Roxanne Tapia, Cliff Han, Alex Copeland, Konstantinos Liolios, Amy Chen, Victor Markowitz, Amrita Pati, Konstantinos Mavromatis, Tanja Woyke, Nikos Kyrpides, Natalia Ivanova, John Gregory Howieson

Grain and Other Field Crops Research Articles

Mesorhizobium australicum strain WSM2073T was isolated from root nodules on the pasture legume Biserrula pelecinus growing in Australia in 2000. This aerobic, motile, gram negative, non-spore-forming rod is poorly effective in N2 fixation on B. pelecinus and has gained the ability to nodulate B. pelecinus following in situ lateral transfer of a symbiosis island from the original inoculant strain for this legume, Mesorhizobium ciceri bv. biserrulae WSM1271. We describe that the genome size of M. australicum strain WSM2073T is 6,200,534 bp encoding 6,013 protein-coding genes and 67 RNA-only encoding genes. This genome does not contain any plasmids but has a …

Complete Genome Sequence Of Mesorhizobium Opportunistum Type Strain Wsm2075t, Wayne Reeve, Kemanthi G. Nandasena, Ronald J. Yates, Ravi P. Tiwari, Graham William O'Hara, Mohamed Ninawi, Olga Chertkov, Lynne Goodwin, David Bruce, Chris Detter, Roxanne Tapia, Shunseng Han, Tanja Woyke, Sam Pitluck, Matt Nolan, Miriam Land, Alex Copeland, Konstantinos Liolios, Amrita Pati, Konstantinos Mavromatis, Victor Markowitz, Nikos Kyrpides, Natalia Ivanova, Uma Meenakshi, John Gregory Howieson

Grain and Other Field Crops Research Articles

Mesorhizobium opportunistum strain WSM2075T was isolated in Western Australia in 2000 from root nodules of the pasture legume Biserrula pelecinus that had been inoculated with M. ciceri bv. biserrulae WSM1271. WSM2075T is an aerobic, motile, Gram negative, non-spore-forming rod that has gained the ability to nodulate B. pelecinus but is completely ineffective in N2 fixation with this host. This report reveals that the genome of M. opportunistum strain WSM2075T contains a chromosome of size 6,884,444 bp, encoding 6,685 protein-coding genes and 62 RNA-only encoding genes. The genome contains no plasmids, but does harbor a 455.7 kb genomic island from Mesorhizobium …

Genome Sequence Of The Lebeckia Ambigua-Nodulating “Burkholderia Sprentiae” Strain Wsm5005ᵀ, Wayne Reeve, Sofie E. De Meyer, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Tian Rui, Ravi P. Tiwari, John Gregory Howieson, Ronald J. Yates, Graham William O'Hara, Megan Lu, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Ernest Szeto, Natalia Ivanova, Natalia Mikhailova, Galina Ovchinikova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Lin Peters, Sam Pitluck, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

“Burkholderia sprentiae” strain WSM5005T is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated in Australia from an effective N2-fixing root nodule of Lebeckia ambigua collected in Klawer, Western Cape of South Africa, in October 2007. Here we describe the features of “Burkholderia sprentiae” strain WSM5005T, together with the genome sequence and its annotation. The 7,761,063 bp high-quality-draft genome is arranged in 8 scaffolds of 236 contigs, contains 7,147 protein-coding genes and 76 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.

Genome Sequence Of The Trifolium Rueppellianum - Nodulating Rhizobium Leguminosarum Bv. Trifolii Strain Wsm2012, Wayne Reeve, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Jason Terpolilli, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Brittany Held, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Ernest Szeto, Natalia Ivanova, Natalia Mikhailova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii WSM2012 (syn. MAR1468) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an ineffective root nodule recovered from the roots of the annual clover Trifolium rueppellianum Fresen growing in Ethiopia. WSM2012 has a narrow, specialized host range for N2-fixation. Here we describe the features of R. leguminosarum bv. trifolii strain WSM2012, together with genome sequence information and annotation. The 7,180,565 bp high-quality-draft genome is arranged into 6 scaffolds of 68 contigs, contains 7,080 protein-coding genes and 86 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint …

Genome Sequence Of The Clover-Nodulating Rhizobium Leguminosarum Bv. Trifolii Strain Ta1, Wayne Reeve, Rui Tian, Sofie E. De Meyer, Vanessa Melino, Jason Terpolilli, Julie Kaye Ardley, Ravi P. Tiwari, John Gregory Howieson, Ronald J. Yates, Graham William O'Hara, Mohamed Ninawi, Hazuki Teshima, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Natalia Ivanova, Galina Ovchinikova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Sam Pitluck, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii strain TA1 is an aerobic, motile, Gram-negative, non-spore-forming rod that is an effective nitrogen fixing microsymbiont on the perennial clovers originating from Europe and the Mediterranean basin. TA1 however is ineffective with many annual and perennial clovers originating from Africa and America. Here we describe the features of R. leguminosarum bv. trifolii strain TA1, together with genome sequence information and annotation. The 8,618,824 bp high-quality-draft genome is arranged in a 6 scaffold of 32 contigs, contains 8,493 protein-coding genes and 83 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the …

Genome Sequence Of The Lupin-Nodulating Bradyrhizobium Sp. Strain Wsm1417, Wayne Reeve, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Hazuki Teshima, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Natalia Ivanova, Galina Ovchinikova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Lin Peters, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Bradyrhizobium sp. strain WSM1417 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen (N2) fixing root nodule of Lupinus sp. collected in Papudo, Chile, in 1995. However, this microsymbiont is a poorly effective N2 fixer with the legume host Lupinus angustifolius L.; a lupin species of considerable economic importance in both Chile and Australia. The symbiosis formed with L. angustifolius produces less than half of the dry matter achieved by the symbioses with commercial inoculant strains such as Bradyrhizobium sp. strain WSM471. Therefore, WSM1417 is an important candidate strain with which to investigate the genetics …

Genome Sequence Of The South American Clover-Nodulating Rhizobium Leguminosarum Bv. Trifolii Strain Wsm597, Wayne Reeve, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Brittany Held, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii strain WSM597 is an aerobic, motile, Gram-negative, non-spore-forming rod isolated from a root nodule of the annual clover Trifolium pallidum L. growing at Glencoe Research Station near Tacuarembó, Uruguay. This strain is generally ineffective for nitrogen (N2) fixation with clovers of Mediterranean, North American and African origin, but is effective on the South American perennial clover T. polymorphum Poir. Here we describe the features of R. leguminosarum bv. trifolii strain WSM597, together with genome sequence information and annotation. The 7,634,384 bp high-quality-draft genome is arranged in 2 scaffolds of 53 contigs, contains 7,394 protein-coding genes and …

Plant Responses To Heterogeneous Salinity: Growth Of The Halophyte Atriplex Nummularia Is Determined By The Root-Weighted Mean Salinity Of The Root Zone, Nadia Bazihizina, E G. Barrett-Lennard, Timothy Colmer

Grain and Other Field Crops Research Articles

Soil salinity is generally spatially heterogeneous, but our understanding of halophyte physiology under such conditions is limited. The growth and physiology of the dicotyledonous halophyte Atriplex nummularia was evaluated in split-root experiments to test whether growth is determined by: (i) the lowest; (ii) the highest; or (iii) the mean salinity of the root zone. In two experiments, plants were grown with uniform salinities or horizontally heterogeneous salinities (10–450mM NaCl in the low-salt side and 670mM in the high-salt side, or 10mM NaCl in the low-salt side and 500–1500mM in the high-salt side). The combined data showed that growth and gas …