Agronomy and Crop Sciences Commons^™

Noodles Made From High Amylose Wheat Flour Attenuate Postprandial Glycaemia In Healthy Adults, Kim Ang, Carla Bourgy, Haelee Fenton, Ahmed Regina, Marcus Newberry, Dean A. Diepeveen, Domenico Lafiandra, Sara Grafenauer, Wendy Hunt, Vicky Solah

Grain and Other Field Crops Research Articles

Previous research has not considered the effect of high amylose wheat noodles on postprandial glycaemia. The aim of the study is to investigate the effect of consumption of high amylose noodles on postprandial glycaemia over 2-h periods by monitoring changes in blood glucose concentration and calculating the total area under the blood glucose concentration curve. Twelve healthy young adults were recruited to a repeated measure randomised, single-blinded crossover trial to compare the effect of consuming noodles (180 g) containing 15%, 20% and 45% amylose on postprandial glycaemia. Fasting blood glucose concentrations were taken via finger-prick blood samples. Postprandial blood glucose …

Tactical Crop Management For Improved Productivity In Winter-Dominant Rainfall Regions: A Review, W K. Anderson, Ross Brennan, Kithsiri W. Jayasena, Svetlana Micic, John H. Moore, T Nordblom

Grain and Other Field Crops Research Articles

This study reviews published information on the tactical management decisions needed to maximise economic grain yield in winter-dominant rainfall regions of the Mediterranean type. Tactical decisions are defined as those relating to the period from immediately before sowing to harvest. Tactical management is the principal means by which farmers respond to changing environmental and short-term economic conditions as the season progresses. The review considers published evidence that underpins these decisions and relates to cereal crops (wheat, barley and oats), pulse crops (field pea, faba bean, chickpea and narrow-leaved lupin) and canola.

The criteria used to guide management decisions during the …

Environmental Adaptation Of Leucaena In Western Australia – Challenges And Opportunities, Clinton K. Revell Dr, Geoff A. Moore Mr, Daniel Real, Sam Crouch

Grain and Other Field Crops Research Articles

There is considerable interest from Western Australian (WA) pastoralists on the potential role of leucaena(Leucaena leucocephala) in northern WA, where the potential area for dryland production of species of the genus Leucaenais high. Although it is highly regarded for animal production in other countries and in Queensland, leucaena is a contentious species since its status as an environmental weed currently precludes it from use on pastoral leases in the Kimberley and Pilbara regions of WA. Development of sterile/seedless forms would overcome risks of spread of the species as a weed. The key environmental constraints to growth of leucaena are likely …

Hybridisation-Based Target Enrichment Of Phenology Genes To Dissect The Genetic Basis Of Yield And Adaptation In Barley, Camilla Beate Hill, Tefera Angessa, Lee-Anne Mcfawn, Debbie Wong, Josquin Tibbits, Xiao-Qi Zhang, Kerrie Forrest, David Moody, Paul Telfer, Sharon Westcott, Dean A. Diepeveen, Yanhao Xu, Cong Tan, Matthew Hayden, Chengdao Li

Grain and Other Field Crops Research Articles

Barley (Hordeum vulgare L.) is a major cereal grain widely used for livestock feed, brewing malts and human food. Grain yield is the most important breeding target for genetic improvement and largely depends on optimal timing of flowering. Little is known about the allelic diversity of genes that underlie flowering time in domesticated barley, the genetic changes that have occurred during breeding, and their impact on yield and adaptation. Here, we report a comprehensive genomic assessment of a worldwide collection of 895 barley accessions based on the targeted resequencing of phenology genes. A versatile target-capture method was used to …

Nam Gene Allelic Composition And Its Relation To Grain-Filling Duration And Nitrogen Utilisation Efficiency Of Australian Wheat, Zaid Alhabbar, Rongchang Yang, Angela Juhasz, Xin Hu, Maoyun She, Masood Anwar, Nigarin Sultana, Dean Diepeveen, Wujun Ma, Shahidul Islam

Grain and Other Field Crops Research Articles

Optimising nitrogen fertiliser management in combination with using high nitrogen efficient wheat cultivars is the most effective strategy to maximise productivity in a cost-efficient manner. The present study was designed to investigate the associations between nitrogen utilisation efficiency (NUtE) and the allelic composition of the NAM genes in Australian wheat cultivars. As results, the non-functional NAM-B1 allele was more responsive to the nitrogen levels and increased NUtE significantly, leading to a higher grain yield but reduced grain protein content. Nitrogen application at different developmental stages (mid-tillering, booting, and flowering) did not show significant differences in grain yield and protein content. …

Wheat Grain Protein Accumulation And Polymerization Mechanisms Driven By Nitrogen Fertilization, Zitong Yu, Shahidul Islam, Maoyun She, Dean Diepeveen, Yujuan Zhang, Guixiang Tang, Jingjuan Zhang, Angela Juhasz, Rongchang Yang, Wujun Ma

Grain and Other Field Crops Research Articles

In wheat (Triticum aestivum) grain yield and grain protein content are negatively correlated, making the simultaneous increase of the two traits challenging. Apart from genetic approaches, modification of nitrogen fertilization offers a feasible option to achieve this aim. In this study, a range of traits related to nitrogen-use efficiency in six Australian bread wheat varieties were investigated under different nitrogen treatments using 3-year multisite field trials. Changes in the individual storage protein composition were detected by high-performance liquid chromatography. Our results indicated that wheat grain yield and grain protein content reacted similarly to nitrogen availability, with grain yield …

Impact Of Mid-Season Sulphur Deficiency On Wheat Nitrogen Metabolism And Biosynthesis Of Grain Protein, Zitong Yu, Angela Juhasz, Shahidul Islam, Dean Diepeveen, Jingjuan Zhang, Penghao Wang, Wujun Ma

Grain and Other Field Crops Research Articles

Wheat (Triticum aestivum) quality is mainly determined by grain storage protein compositions. Sulphur availability is essential for the biosynthesis of the main wheat storage proteins. In this study, the impact of different sulphur fertilizer regimes on a range of agronomically important traits and associated gene networks was studied. High-performance liquid chromatography was used to analyse the protein compositions of grains grown under four different sulphur treatments. Results revealed that sulphur supplementation had a significant effect on grain yield, harvest index, and storage protein compositions. Consequently, two comparative sulphur fertilizer treatments (0 and 30 kg ha−1 sulphur, with 50 …

Early Growth Stages Salinity Stress Tolerance In Cm72 X Gairdner Doubled Haploid Barley Population, Tefera T. Angessa, Xiao-Qi Zhang, Gaofeng Zhou, Sue Broughton, Wenying Zhang, Chengdao Li

Grain and Other Field Crops Research Articles

A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H–6H each …

Zernike Moments And Genetic Algorithm: Tutorial And Application, Oluleye Babatunde, Leisa Armstrong, Jinsong Leng, Dean Diepeveen

Grain and Other Field Crops Research Articles

Aims/ objectives: To demontrate effectiveness of Zernike Moments for Image Classification. Zernike moment(ZM) is an excellent region-based moment which has attracted the attentions of many image processing researchers since its first application to image analysis. Many papers have been published on several works done on ZM but no single paper ever give a detailed information of how the computation of ZM is done from the time the image is captured to the computation of ZM. This work showed how to effectively apply ZM on RGB images. We have demonstrated the effectiveness of Zernike moment in image classification system. A neuro-genetic …

A Neuronal Classification System For Plant Leaves Using Genetic Image Segmentation, Oluleye Babatunde, Leisa Armstrong, Dean Diepeveen, Jinsong Leng

Grain and Other Field Crops Research Articles

This paper demonstrates the use of radial basis networks (RBF), cellular neural networks (CNN)and genetic algorithm (GA) for automatic classification of plant leaves. A genetic neuronal system herein attempted to solve some of the inherent challenges facing current software being employed for plant leaf classification. The image segmentation module in this work was genetically optimized to bring salient features in the images of plants leaves used in this work. The combination of GA-based CNN with RBF in this work proved more efficient than the existing systems that use conventional edge operators such as Canny, LoG, Prewitt, and Sobel operators. The …

Potential Break Crops For Managing Root-Knot Nematode, Carla Wilkinson, Stuart Vincent, Sarah J. Collins Dr

Grain and Other Field Crops Research Articles

Western Australian growers may be unintentionally increasing root-knot nematode, Meloidogyne fallax (False Columbia root-knot nematode) populations by using susceptible break crops between cash crops.

A Genetic Algorithm-Based Feature Selection, Oluleye Babatunde, Leisa Armstrong, Jinsong Leng, Dean Diepeveen

Grain and Other Field Crops Research Articles

This article details the exploration and application of Genetic Algorithm (GA) for feature selection. Particularly a binary GA was used for dimensionality reduction to enhance the performance of the concerned classifiers. In this work, hundred (100) features were extracted from set of images found in the Flavia dataset (a publicly available dataset). The extracted features are Zernike Moments (ZM), Fourier Descriptors (FD), Lengendre Moments (LM), Hu 7 Moments (Hu7M), Texture Properties (TP) and Geometrical Properties (GP). The main contributions of this article are (1) detailed documentation of the GA Toolbox in MATLAB and (2) the development of a GA-based feature …

Genome Sequence Of The Listia Angolensis Microsymbiont Microvirga Lotononidis Strain Wsm3557ᵀ, Wayne Reeve, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Jason Terpolilli, Vanessa Melino, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Xiaojing Zhang, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Ernest Szeto, Natalia Ivanova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Microvirga lotononidis is a recently described species of root-nodule bacteria that is an effective nitrogen- (N2) fixing microsymbiont of the symbiotically specific African legume Listia angolensis (Welw. ex Bak.) B.-E. van Wyk & Boatwr. M. lotononidis possesses several properties that are unusual in root-nodule bacteria, including pigmentation and the ability to grow at temperatures of up to 45°C. Strain WSM3557T is an aerobic, motile, Gram-negative, non-spore-forming rod isolated from a L. angolensis root nodule collected in Chipata, Zambia in 1963. This is the first report of a complete genome sequence for the genus Microvirga. Here we describe the features …

Genome Sequence Of Rhizobium Leguminosarum Bv Trifolii Strain Wsm1689, The Microsymbiont Of The One Flowered Clover Trifolium Uniflorum, Jason Terpolilli, Tian Rui, Ronald J. Yates, John Gregory Howieson, Philip Poole, Christine Munk, Roxanne Tapia, Cliff Han, Victor Markowitz, Reddy Tatiparthi, Konstantinos Mavromatis, Natalia Ivanova, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides, Wayne Reeve

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii is a soil-inhabiting bacterium that has the capacity to be an effective N2-fixing microsymbiont of Trifolium (clover) species. R. leguminosarum bv. trifolii strain WSM1689 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Trifolium uniflorum collected on the edge of a valley 6 km from Eggares on the Greek Island of Naxos. Although WSM1689 is capable of highly effective N2-fixation with T. uniflorum, it is either unable to nodulate or unable to fix N2 with a wide range of both perennial and annual clovers originating from Europe, North America …

Complete Genome Sequence Of Mesorhizobium Australicum Type Strain (Wsm2073t), Wayne Reeve, Kemanthi G. Nandasena, Ronald J. Yates, Ravi P. Tiwari, Graham William O'Hara, Mohamed Ninawi, Wei Gu, Lynne Goodwin, Chris Detter, Roxanne Tapia, Cliff Han, Alex Copeland, Konstantinos Liolios, Amy Chen, Victor Markowitz, Amrita Pati, Konstantinos Mavromatis, Tanja Woyke, Nikos Kyrpides, Natalia Ivanova, John Gregory Howieson

Grain and Other Field Crops Research Articles

Mesorhizobium australicum strain WSM2073T was isolated from root nodules on the pasture legume Biserrula pelecinus growing in Australia in 2000. This aerobic, motile, gram negative, non-spore-forming rod is poorly effective in N2 fixation on B. pelecinus and has gained the ability to nodulate B. pelecinus following in situ lateral transfer of a symbiosis island from the original inoculant strain for this legume, Mesorhizobium ciceri bv. biserrulae WSM1271. We describe that the genome size of M. australicum strain WSM2073T is 6,200,534 bp encoding 6,013 protein-coding genes and 67 RNA-only encoding genes. This genome does not contain any plasmids but has a …

Genome Sequence Of The Trifolium Rueppellianum - Nodulating Rhizobium Leguminosarum Bv. Trifolii Strain Wsm2012, Wayne Reeve, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Jason Terpolilli, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Brittany Held, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Ernest Szeto, Natalia Ivanova, Natalia Mikhailova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii WSM2012 (syn. MAR1468) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an ineffective root nodule recovered from the roots of the annual clover Trifolium rueppellianum Fresen growing in Ethiopia. WSM2012 has a narrow, specialized host range for N2-fixation. Here we describe the features of R. leguminosarum bv. trifolii strain WSM2012, together with genome sequence information and annotation. The 7,180,565 bp high-quality-draft genome is arranged into 6 scaffolds of 68 contigs, contains 7,080 protein-coding genes and 86 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint …

Genome Sequence Of The Lupin-Nodulating Bradyrhizobium Sp. Strain Wsm1417, Wayne Reeve, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Hazuki Teshima, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Natalia Ivanova, Galina Ovchinikova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Lin Peters, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Bradyrhizobium sp. strain WSM1417 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen (N2) fixing root nodule of Lupinus sp. collected in Papudo, Chile, in 1995. However, this microsymbiont is a poorly effective N2 fixer with the legume host Lupinus angustifolius L.; a lupin species of considerable economic importance in both Chile and Australia. The symbiosis formed with L. angustifolius produces less than half of the dry matter achieved by the symbioses with commercial inoculant strains such as Bradyrhizobium sp. strain WSM471. Therefore, WSM1417 is an important candidate strain with which to investigate the genetics …

Genome Sequence Of The Ornithopus/Lupinus-Nodulating Bradyrhizobium Sp. Strain Wsm471, Wayne Reeve, Sofie E. De Meyer, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Ravi P. Tiwari, John Gregory Howieson, Ronald J. Yates, Graham William O'Hara, Mohamed Ninawi, Megan Lu, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Natalia Ivanova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Bradyrhizobium sp. strain WSM471 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen- (N2) fixing root nodule formed on the annual legume Ornithopus pinnatus (Miller) Druce growing at Oyster Harbour, Albany district, Western Australia in 1982. This strain is in commercial production as an inoculant for Lupinus and Ornithopus. Here we describe the features of Bradyrhizobium sp. strain WSM471, together with genome sequence information and annotation. The 7,784,016 bp high-quality-draft genome is arranged in 1 scaffold of 2 contigs, contains 7,372 protein-coding genes and 58 RNA-only encoding genes, and is one of 20 rhizobial …

Genome Sequence Of The Clover-Nodulating Rhizobium Leguminosarum Bv. Trifolii Strain Ta1, Wayne Reeve, Rui Tian, Sofie E. De Meyer, Vanessa Melino, Jason Terpolilli, Julie Kaye Ardley, Ravi P. Tiwari, John Gregory Howieson, Ronald J. Yates, Graham William O'Hara, Mohamed Ninawi, Hazuki Teshima, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Natalia Ivanova, Galina Ovchinikova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Sam Pitluck, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii strain TA1 is an aerobic, motile, Gram-negative, non-spore-forming rod that is an effective nitrogen fixing microsymbiont on the perennial clovers originating from Europe and the Mediterranean basin. TA1 however is ineffective with many annual and perennial clovers originating from Africa and America. Here we describe the features of R. leguminosarum bv. trifolii strain TA1, together with genome sequence information and annotation. The 8,618,824 bp high-quality-draft genome is arranged in a 6 scaffold of 32 contigs, contains 8,493 protein-coding genes and 83 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the …

Complete Genome Sequence Of Mesorhizobium Opportunistum Type Strain Wsm2075t, Wayne Reeve, Kemanthi G. Nandasena, Ronald J. Yates, Ravi P. Tiwari, Graham William O'Hara, Mohamed Ninawi, Olga Chertkov, Lynne Goodwin, David Bruce, Chris Detter, Roxanne Tapia, Shunseng Han, Tanja Woyke, Sam Pitluck, Matt Nolan, Miriam Land, Alex Copeland, Konstantinos Liolios, Amrita Pati, Konstantinos Mavromatis, Victor Markowitz, Nikos Kyrpides, Natalia Ivanova, Uma Meenakshi, John Gregory Howieson

Grain and Other Field Crops Research Articles

Mesorhizobium opportunistum strain WSM2075T was isolated in Western Australia in 2000 from root nodules of the pasture legume Biserrula pelecinus that had been inoculated with M. ciceri bv. biserrulae WSM1271. WSM2075T is an aerobic, motile, Gram negative, non-spore-forming rod that has gained the ability to nodulate B. pelecinus but is completely ineffective in N2 fixation with this host. This report reveals that the genome of M. opportunistum strain WSM2075T contains a chromosome of size 6,884,444 bp, encoding 6,685 protein-coding genes and 62 RNA-only encoding genes. The genome contains no plasmids, but does harbor a 455.7 kb genomic island from Mesorhizobium …

Genome Sequence Of The Lebeckia Ambigua-Nodulating “Burkholderia Sprentiae” Strain Wsm5005ᵀ, Wayne Reeve, Sofie E. De Meyer, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Tian Rui, Ravi P. Tiwari, John Gregory Howieson, Ronald J. Yates, Graham William O'Hara, Megan Lu, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han, I-Min Chen, Konstantinos Mavromatis, Victor Markowitz, Ernest Szeto, Natalia Ivanova, Natalia Mikhailova, Galina Ovchinikova, Ioanna Pagani, Amrita Pati, Lynne Goodwin, Lin Peters, Sam Pitluck, Tanja Woyke, Nikos Kyrpides

Grain and Other Field Crops Research Articles

“Burkholderia sprentiae” strain WSM5005T is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated in Australia from an effective N2-fixing root nodule of Lebeckia ambigua collected in Klawer, Western Cape of South Africa, in October 2007. Here we describe the features of “Burkholderia sprentiae” strain WSM5005T, together with the genome sequence and its annotation. The 7,761,063 bp high-quality-draft genome is arranged in 8 scaffolds of 236 contigs, contains 7,147 protein-coding genes and 76 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.

Genome Sequence Of The South American Clover-Nodulating Rhizobium Leguminosarum Bv. Trifolii Strain Wsm597, Wayne Reeve, Jason Terpolilli, Vanessa Melino, Julie Kaye Ardley, Rui Tian, Sofie E. De Meyer, Ravi P. Tiwari, Ronald J. Yates, Graham William O'Hara, John Gregory Howieson, Mohamed Ninawi, Brittany Held, David Bruce, Chris Detter, Roxanne Tapia, Cliff Han, Chia-Lin Wei, Marcel Huntemann, James Han

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv. trifolii strain WSM597 is an aerobic, motile, Gram-negative, non-spore-forming rod isolated from a root nodule of the annual clover Trifolium pallidum L. growing at Glencoe Research Station near Tacuarembó, Uruguay. This strain is generally ineffective for nitrogen (N2) fixation with clovers of Mediterranean, North American and African origin, but is effective on the South American perennial clover T. polymorphum Poir. Here we describe the features of R. leguminosarum bv. trifolii strain WSM597, together with genome sequence information and annotation. The 7,634,384 bp high-quality-draft genome is arranged in 2 scaffolds of 53 contigs, contains 7,394 protein-coding genes and …

Plant Responses To Heterogeneous Salinity: Growth Of The Halophyte Atriplex Nummularia Is Determined By The Root-Weighted Mean Salinity Of The Root Zone, Nadia Bazihizina, E G. Barrett-Lennard, Timothy Colmer

Grain and Other Field Crops Research Articles

Soil salinity is generally spatially heterogeneous, but our understanding of halophyte physiology under such conditions is limited. The growth and physiology of the dicotyledonous halophyte Atriplex nummularia was evaluated in split-root experiments to test whether growth is determined by: (i) the lowest; (ii) the highest; or (iii) the mean salinity of the root zone. In two experiments, plants were grown with uniform salinities or horizontally heterogeneous salinities (10–450mM NaCl in the low-salt side and 670mM in the high-salt side, or 10mM NaCl in the low-salt side and 500–1500mM in the high-salt side). The combined data showed that growth and gas …

Next Generation Dna Sequencing Technology Delivers Valuable Genetic Markers For The Genomic Orphan Legume Species, Bituminaria Bituminosa, M Pazos-Navarro, Mercedes Dabauza, Enrique Correal, Kelly Hanson, Natasha L. Teakle, Daniel Real, Matthew Nelson

Grain and Other Field Crops Research Articles

Background
Bituminaria bituminosa is a perennial legume species from the Canary Islands and Mediterranean region that has potential as a drought-tolerant pasture species and as a source of pharmaceutical compounds. Three botanical varieties have previously been identified in this species: albomarginata, bituminosa and crassiuscula. B. bituminosa can be considered a genomic 'orphan' species with very few genomic resources available. New DNA sequencing technologies provide an opportunity to develop high quality molecular markers for such orphan species.

Results
432,306 mRNA molecules were sampled from a leaf transcriptome of a single B. bituminosa plant using Roche 454 pyrosequencing, resulting in an …

Phenological Development In Two-Row Spring Barley When Grown In A Long-Day (Alberta, Canada) And A Short-Day (Western Australia, Australia) Environment, Blakely Paynter, P E. Juskiw, J H. Helm

Grain and Other Field Crops Research Articles

Phenological development in eight cultivars of two-row, spring barley (Hordeum vulgare L.) was examined when sown at two dates of seeding in two diverse environments. These environments were a short-day environment at Northam, Western Australia, Australia, in 1997 and a long-day environment at Lacombe, Alberta, Canada, in 1998. The Australian and Canadian barley cultivars used had different combinations of basic vegetative phase and daylength sensitivity. Barley grown at Lacombe reached each stage of phenological development in less time than at Northam. Most noticeable was a shorter duration of the period between seedling emergence to double ridge and between double ridge …

Evaluation Of Perennial Forage Legumes And Herbs In Six Mediterranean Environments, Daniel Real, Guangdi D. Li, Steve Clark, Tony Albertsen, Richard C. Hayes, Matt D. Denton, Mario F. D'Antuono, B S. Dear

Grain and Other Field Crops Research Articles

There is an absence of drought tolerant herbaceous perennial forage legume and herb options other than lucerne (Medicago sativa L.) for environments with Mediterranean-like climates common in extensive areas of Southern Australia, the Mediterranean basin, and Chile. Therefore, a collection of 174 forage perennial legume and herb entries from 103 species and 32 genera was evaluated for adaptation in a diverse range of Mediterranean climatic environments in Southern Australia. The seasonal rainfall distribution varied from moderately to highly winter dominant with long term average annual rainfall ranging from 318 to 655 mm. The entries were rated for productivity and …

Selecting Improved Lotus Nodulating Rhizobia To Expedite The Development Of New Forage Species, John Gregory Howieson, Ross Andrew Ballard, Ronald J. Yates, Nigel Charman

Grain and Other Field Crops Research Articles

Aims

In the past decades the increasing focus by Australian pasture development programs on the genus Lotus has seen the evaluation of many species previously untested in Australia. In field trials, nodulation failure was commonplace. This work was undertaken to select effective symbionts for Lotus to ensure further agronomic evaluation of the genus was not compromised. The symbiotic needs of Lotus ornithopodioides were a particular focus of the studies.

Methods

Glasshouse experiments were undertaken to evaluate symbiotic relationships between 15 Lotus spp and 23 strains of nodulating Mesorhizobium loti. This was followed by evaluation of elite rhizobial strains for …

Complete Genome Sequence Of Rhizobium Leguminosarum Bv. Trifolii Strain Wsm1325, An Effective Microsymbiont Of Annual Mediterranean Clovers, Wayne Reeve, Graham William O'Hara, Patrick Chain, Julie Kaye Ardley, Lambert Brau, Kemanthi Nandesena, Ravi P. Tiwari, Alex Copeland, Matt Nolan, Cliff Han, Thomas Brettin, Miriam Land, Galina Ovchinikova, Natalia Ivanova, Konstantinos Mavromatis, Victor Markowitz, Nikos Kyrpides, Vanessa Melino, Matt D. Denton, Ronald J. Yates, John Howieson

Grain and Other Field Crops Research Articles

Rhizobium leguminosarum bv trifolii is a soil-inhabiting bacterium that has the capacity to be an effective nitrogen fixing microsymbiont of a diverse range of annual Trifolium (clover) species. Strain WSM1325 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from root nodules collected in 1993 from the Greek Island of Serifos. WSM1325 is produced commercially in Australia as an inoculant for a broad range of annual clovers of Mediterranean origin due to its superior attributes of saprophytic competence, nitrogen fixation and acid-tolerance. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This …

Leaf Development In Two-Row Spring Barley Under Long-Day And Short-Day Field Conditions, Blakely Paynter, P E. Juskiw, J H. Helm

Grain and Other Field Crops Research Articles

To gain an understanding of the adaptation of Australian and Canadian barley (Hordeum vulgare L.) cultivars to the environments of western Canada and Western Australia, phyllochron and number of leaves on the mainstem in eight cultivars of two-row, spring barley were examined when sown at two dates in two locations. The locations were a short-day environment at Northam, Western Australia, Australia in 1997 and a long-day environment at Lacombe, Alberta, Canada in 1998. At each location highly significant relationships between leaf number on the mainstem and thermal time were found (r2 > 0.94). Using linear estimates, the phyllochron of barley under …

Comparison Of The Phosphate Requirements Of Burr Medic And Yellow Serradella With That Of Wheat In The Low Rainfall Wheatbelt Of Western Australia, Blakely Paynter, Mike D. Bolland

Grain and Other Field Crops Research Articles

The responses of burr medic (Medicago polymorpha) and yellow serradella (Ornithopus compressus) to topdressed phosphate were compared with the response of wheat (Triticum aestivum) to drilled phosphate on a range of soils in the low rainfall wheatbelt of Western Australia. Species were compared firstly on the amount of applied phosphorus (P) required for 90% maximum yield at 3 harvests, and secondly on the ratio of their curvature coefficients from the Mitscherlich relationship between absolute yield and P applied. On 7 marginally acidic, medium-textured soils, the comparative requirements of burr medic (cvv. Serena or Santiago) and wheat (cv. Gutha) for applied …