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Articles 1 - 5 of 5
Full-Text Articles in Cellular and Molecular Physiology
Ammonia Regulation In Aedes Taeniorhynchus, A Salt-Water Mosquito, Joseph Edmonds
Ammonia Regulation In Aedes Taeniorhynchus, A Salt-Water Mosquito, Joseph Edmonds
Undergraduate Honors Theses
The salt tolerant mosquito species Aedes taeniorhynchus has been observed in high salinity and ammonia conditions. Previous studies indicate Na+/K+ ATPase (NKA), the proton pump vacuolar-type H+ ATPase (VHA) and sodium-dependent cation-chloride cotransporters (NKCC’s) in sodium secretion in larval Aedes taeniorhynchus. However ammonia transporter such as Rh-protein, Amt1, and Amt2 have only been characterized in Aedes taeniorhynchus’s fresh water relative Aedes aegypti. Our research exhibits the expression of these transporters in A.taeniorhynchus using immunohistochemistry techniques. Evidence of VHA and NKA function in ammonia transport was characterized in vivo using drug antagonists in varying saline and ammonia concentrations. Ammonia flux rates …
Pioglitazone, Neet Family Proteins, And Galactose Modulation Of Liver Cell Bioenergetics, David F. Grimm
Pioglitazone, Neet Family Proteins, And Galactose Modulation Of Liver Cell Bioenergetics, David F. Grimm
Undergraduate Honors Theses
MitoNEET was discovered through interactions with a labeled and photoactive derivative of pioglitazone (pio), a drug used to increase peripheral insulin sensitivity. Its unique coordination of a [2Fe-2S] cluster by three cysteine residues (Cys-72, Cys-74, and Cys-83) and one histidine (His-87) gives this cluster both stability and the ability to be donated to acceptor proteins. These qualities allow mitoNEET to participate in a diversity of biological functions. Functions of mitoNEET and the consequences of pioglitazone (pio) treatment in human hepatocellular carcinoma (HepG2) cells cultured in glucose or galactose-based medium were examined by respiration and proliferation studies. Pio treatment decreased complex …
Regulation Of Dkk1, An Inhibitor Of The Wnt Signaling Pathway, By Dax-1 In Mouse Embryonic Stem Cells, Victor D. Gavallos
Regulation Of Dkk1, An Inhibitor Of The Wnt Signaling Pathway, By Dax-1 In Mouse Embryonic Stem Cells, Victor D. Gavallos
Undergraduate Honors Theses
DAX-1 is known to play a key role in the maintenance of pluripotency in mouse embryonic stem cells (mESC), such that elimination of DAX-1 (through methods such as RNAi) leads to the differentiation of ESC (Khalfallah et al. 2009). Earlier research by the Tzagarakis-Foster lab identified target genes of DAX-1 in mESC through PCR array methodology (Torres 2013). The Dickkopf 1 gene (Dkk1) was identified as a potential target of DAX-1 by these studies and has since been confirmed to be a DAX-1 target in mESC. However, to date very little is understood of the mechanism of DAX-1 repression of …
Eggshell Calcium Regulates Calcium Transport Protein Expression In An Oviparous Snake, Hannah Frye
Eggshell Calcium Regulates Calcium Transport Protein Expression In An Oviparous Snake, Hannah Frye
Undergraduate Honors Theses
One hypothesis explaining the numerous independent evolutionary transitions from oviparity to viviparity among squamates (snakes and lizards) proposed that squamate embryonic development is independent of eggshell calcium. Recent research showed at least 25% of the calcium in hatchling oviparous squamates is extracted from the shell. Though not a direct test, these results are inconsistent with the hypothesis. To directly test the hypothesis, we removed eggshell calcium (through peeling) early in development of Pantherophis guttatus (corn snake) eggs. Survivorship to hatching did not differ between peeled and intact eggs. Yet hatchlings from peeled eggs were shorter (273.6 ± 3.4 vs. 261.0 …
Mitoneet: Reduction In Insulin Resistance Through Ameliorated Oxidative Stress?, Nicolas A. Ferry
Mitoneet: Reduction In Insulin Resistance Through Ameliorated Oxidative Stress?, Nicolas A. Ferry
Undergraduate Honors Theses
MitoNEET is a mitochondrial iron-sulfur protein with poorly-understood functions. Proposed functions of mitoNEET include regulating of oxidative capacity, reactive oxygen homeostasis, and other possible roles. Investigating the interactions of mitoNEET with other proteins might help identify functional roles of this protein. MitoNEET was discovered in 2004 to identify physiological targets of pioglitazone hydrochloride, a drug that is commonly used to treat type-2 diabetes. Type-2 diabetes is a metabolic disease and, although the exact pathology is complex, the role of the mitochondrion in cellular energy production and glucose oxidation indicates that this organelle has a role in type-2 diabetes. Because of …