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Master's Theses

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Full-Text Articles in Physiology

Comparative Thermal Ecology Of Coastal And Inland Pacific Rattlesnakes (Crotalus Oreganus), Hayley Layne Crowell May 2019

Comparative Thermal Ecology Of Coastal And Inland Pacific Rattlesnakes (Crotalus Oreganus), Hayley Layne Crowell

Master's Theses

Global biodiversity is declining as a direct result of anthropogenic climate change. Ectothermic species have become focal organisms for studying the ecological effects of altered climates due to the clear relationship between environmental temperatures and ectotherms’ basic physiological functions. Historically, examinations of these effects have focused heavily on heliothermic lizards, and most others have tended to focus on single populations or sympatric species within a single community. Addressing the longterm energetic implications of environmental temperature variation will provide valuable insight into the cascading physiological effects that certain populations or species may experience as a result of altered climates.

In this …


Adolescent Binge Alcohol Exposure Effects On Heart Structure And Function, Lizhuo Ai Jan 2019

Adolescent Binge Alcohol Exposure Effects On Heart Structure And Function, Lizhuo Ai

Master's Theses

More than 5 million underage Americans report binge drinking at least once per month. While the effects of alcohol consumption on the adult heart have been well studied, the impact to the adolescent heart is almost entirely unknown. Adolescents primarily consume alcohol in a binge pattern, which elevates blood alcohol content (BAC) to 0.08 g/dL within 2 hours. During adolescence the body grows rapidly, and the heart must also grow by cellular hypertrophy to meet this increasing demand. Our goal was to determine the impact of adolescent binge alcohol exposure on the heart, using an outbred rat model. This thesis …


The Thermal Ecology Of Sceloporus Occidentalis, Luis Patricio Burgos Dec 2018

The Thermal Ecology Of Sceloporus Occidentalis, Luis Patricio Burgos

Master's Theses

With temperatures rising globally, assessing the possible impacts of the changing climate becomes more and more urgent. Ectotherms are excellent indicators of potential climatic ramifications on biodiversity because of their heavy reliance on the environment for their thermoregulation. Studies have historically looked at thermal tolerance values to establish predictive models for population and species extinctions.

In chapter 1, we looked at recent studies that suggest that thermal tolerance may be a plastic trait and test the effects empirically. Most studies are based on captive lizards acclimated to laboratory conditions that do not necessarily reflect natural environments, and if thermal tolerance …


Physiological Response Of The Giant Acorn Barnacle, Balanus Nubilus, To Air Exposure, Emily Jane Resner Nov 2018

Physiological Response Of The Giant Acorn Barnacle, Balanus Nubilus, To Air Exposure, Emily Jane Resner

Master's Theses

The giant acorn barnacle, Balanus nubilus, is a resident of the subtidal and low intertidal rocky shoreline on the Pacific Coast of North America (Alaska to Baja California). B. nubilusis notable for having the largest muscle fibers in the animal kingdom; fiber diameters that can exceed 3mm in adults! At such extreme sizes these muscle cells may be at risk for insufficient oxygen delivery to mitochondria owing to low SA:V ratios and long intracellular diffusion distances. Oxygen limitation to these muscles may be further exacerbated during low tide air exposure (emersion) or environmental hypoxia events, which are increasing …


The Effects Of Dietary Nitrate Supplementation On Swimming Performance In Collegiate Swimmers 2018, Christopher Daniel Mosconi May 2018

The Effects Of Dietary Nitrate Supplementation On Swimming Performance In Collegiate Swimmers 2018, Christopher Daniel Mosconi

Master's Theses

The desire for performance enhancement is a common goal amongst athletes. Recently a number of studies have reported various performance enhancing effects of dietary nitrate (NO3-) via beetroot juice (BRJ) supplementation. Research has indicated that the various performance enhancing effects of BRJ can be attributed to a decrease resting systolic blood pressure (SBP), diastolic blood pressure (DBP), and submaximal volume of oxygen consumption (VO2), while increasing vasodilation and mitochondrial efficiency. Purpose: The purpose of this study was to investigate whether BRJ supplementation improves swimming performance in collegiate level swimmers. Methods: Eight collegiate trained swimmers underwent a double-blind 5-day chronic supplementation …


Aspects Of Weight Management In Ncaa Division Iii Collegiate Wrestlers 2018, Matthew Martone B.S., Cscs May 2018

Aspects Of Weight Management In Ncaa Division Iii Collegiate Wrestlers 2018, Matthew Martone B.S., Cscs

Master's Theses

Objective: The purpose of this study was to observe the weight management habits of division III collegiate wrestlers during the week leading up to a competition. Methods: Subjects reported to the lab on Monday morning after an overnight fast. Subjects were measured for height and weight and were asked to provide urine and blood samples for analysis of hydration and creatine kinase activity, respectively. Throughout the week, subjects were weighed in and out of practice to monitor daily weight fluctuations. Subjects also self-recorded all food and drink consumption throughout the 5-day period. On Saturday morning, approximately 1 hour before weigh-ins …


The Effectiveness Of Men's Lacrosse Shoulder Pads In The Attenuation Of Linear Impact Forces 2018, Ryan S. Dambach May 2018

The Effectiveness Of Men's Lacrosse Shoulder Pads In The Attenuation Of Linear Impact Forces 2018, Ryan S. Dambach

Master's Theses

BACKGROUND/PURPOSE: Men’s lacrosse is a sport that is growing in popularity. The mandatory shoulder pads have no limit of use standards and are rarely studied. The purpose of the study was to measure peak impact force (IF) of new and used lacrosse shoulder pads. METHODS: Six lacrosse shoulder pads, three new models (Warrior MPG 8.0 shoulder pad, STX Jolt shoulder pad and Warrior Player’s Club Hitlyte shoulder pad) and three used models of the same shoulder pads with at least four seasons of use (Warrior MPG shoulder pad, STX Jolt shoulder pad and Warrior Player’s Club Hitlyte shoulder pad) were …


The Effects Of A Low Dose Of Caffeine On Bat Swing Performance In Fatigued And Non-Fatigued Female Softball Athletes 2018, Brianna Ferchen May 2018

The Effects Of A Low Dose Of Caffeine On Bat Swing Performance In Fatigued And Non-Fatigued Female Softball Athletes 2018, Brianna Ferchen

Master's Theses

Objective: The purpose of this study was to determine if caffeine ingestion decreased time to contact (TTC), and increased peak hand speed (HS), peak bat barrel speed (BS), and power (P) during a softball bat swing by fatigued and non-fatigued female athletes from an NCAA Division III varsity softball team. Methods: A randomized, single blind counterbalanced design was used to determine if 200 mg of caffeinated gum produced an ergogenic effect on nine female softball players (mean ± SD; age: 19.4 ± 0.7 yrs; height: 169 ± 6 cm; weight: 76.85 ± 10.82 kg) during a softball swing after chewing …


Effects Of Competition Variables And Prior Attempts On Approach Run Velocity During A Pole Vaulter's Final Clearance 2018, Nathaniel S. Ashton May 2018

Effects Of Competition Variables And Prior Attempts On Approach Run Velocity During A Pole Vaulter's Final Clearance 2018, Nathaniel S. Ashton

Master's Theses

Approach run velocity of a vaulter is strongly correlated to the highest height a vaulter clears in pole vault competition and the number of attempts taken throughout a competition influences pole vault strategy. Since approach run velocity greatly affects the crossbar height cleared and number of attempts affects time spent in the competition, perhaps a better approach to determine optimal competition strategy is to first identify how competition variables influence approach run velocity. The purpose of this study was to determine if the approach run velocity during a pole vaulter’s last clearance can be predicted by: (1) the number of …


The Relationship Between Stryd Power And Running Economy In Well-Trained Distance Runners 2018, Casey Austin May 2018

The Relationship Between Stryd Power And Running Economy In Well-Trained Distance Runners 2018, Casey Austin

Master's Theses

A novel running wearable called the Stryd Summit footpod attaches to a runner’s right or left shoe and measures running power output. The developers of the product purport that the footpod’s power and form power measures may correlate with metabolic data gathered in a lab. PURPOSE: Explore the relationship between power output and running economy at threshold pace. METHODS: Seventeen well-trained distance runners, 9 males and 8 females, completed a running protocol at threshold pace. Participants ran two discontinuous four-minute stages: one with their self-selected cadence (SS), and one with cadence lowered by 10% (LC). Metabolic data, power, and form …


The Effects Of A Short-Term, Unilateral, Lower-Body Resistance Training Program On Balance In College-Aged Resistance-Trained Participants 2018., Daniel Semprini May 2018

The Effects Of A Short-Term, Unilateral, Lower-Body Resistance Training Program On Balance In College-Aged Resistance-Trained Participants 2018., Daniel Semprini

Master's Theses

Purpose: The purpose of this study was to investigate whether a short-term, unilateral, lower-body resistance training program would significantly improve static and dynamic balance in experienced college-aged resistance-trained participants when compared to a control group’s regular, bilateral lower-body resistance training program. Participants: A total of twenty-four participants were recruited to participate in the study. Four participants ended up dropping out due to injury and time constraints, leaving the final total sample size at twenty. Methods: Participants completed a series of three questionnaires (International Fitness Scale, International Physical Activity, and Sociodemographic Questionnaire) and the informed consent. The participants were randomly divided …


Learning To Run On An Lbppt: Neuromuscular And Metabolic Adaptations 2018, Jordyn Naylon May 2018

Learning To Run On An Lbppt: Neuromuscular And Metabolic Adaptations 2018, Jordyn Naylon

Master's Theses

Previous research on lower body positive pressure (LBPP) treadmills have focused primarily on muscle activation (EMG), spatiotemporal variables, and metabolic differences from running on a standard treadmill. The current study aimed to establish the necessary accommodation time needed for runners to display reliable metabolic and neuromuscular responses on the LBPP treadmill. Fifteen habitually trained runners (11 males, 4 females, average± SD VO2Max=62.81 ± 5.43mL.kg-1.min-1) ran a total of 3, 15-minute trials on the AlterG® treadmill during a single testing session. For each trial, the AlterG® was set to support 30% of body weight at 70% of the speed at which …


Relationships Among Actual Motor Competence, Perceived Motor Competence And Health-Related Fitness In A College-Aged Population 2018, Samantha Moss May 2018

Relationships Among Actual Motor Competence, Perceived Motor Competence And Health-Related Fitness In A College-Aged Population 2018, Samantha Moss

Master's Theses

Purpose: The purpose of this study was to assess relationships among actual motor competence, perceived motor competence, and health-related fitness in a college-aged population. Methods: A total of 76 participants from SUNY Cortland enrolled in an undergraduate Kinesiology course completed an informed consent form. Total data were obtained on 71 participants (male = 53; female = 18). Perceived motor competence (PMC) was assessed via the Physical Self Perception Profile questionnaire, which participants completed one week prior to remaining assessments. Motor competence (MC) was assessed by maximum throw and kick speed as well as maximum distance jumped. Health-related fitness (HRF) was …


Comparative Proteomics: Assessing The Variation In Molecular Physiology Within The Adductor Muscle Between Mytilus Galloprovincialis And Mytilus Trossulus In Response To Acute Heat Stress, Joshua Scott Mier Mar 2018

Comparative Proteomics: Assessing The Variation In Molecular Physiology Within The Adductor Muscle Between Mytilus Galloprovincialis And Mytilus Trossulus In Response To Acute Heat Stress, Joshua Scott Mier

Master's Theses

Increases in seawater temperatures have imposed physiological constraints which are partially thought to contribute to recently observed shifts in biogeographic distribution among closely related intertidal ectotherms. For instance, Mytilus galloprovincialis an introduced warm-adapted species from the Mediterranean, has displaced the native cold-adapted congener, M. trossulus, over large latitudinal expanses off the California coast. Several comparative physiological studies have revealed interspecific differences in thermal tolerance, including variation in aerobic metabolism and gape behavior, which suggest the invasive congener is better adapted to acclimate to increasing seawater conditions as predicted due to climate change. However, current analyses seek to discover the …


Effects Of Temperature On Growth And Molting In Blue Crabs (Callinectes Sapidus) And Lesser Blue Crabs (Callinectes Similis), Abigail Ann Kuhn Dec 2017

Effects Of Temperature On Growth And Molting In Blue Crabs (Callinectes Sapidus) And Lesser Blue Crabs (Callinectes Similis), Abigail Ann Kuhn

Master's Theses

Temperature can exert impacts on many processes in ectotherms. With global temperatures rising due to climate change, many ectothermic species may exhibit changes in growth rates and size at maturity, and these changes can have population-level effects. Predicting responses of species to climate change will require not only knowledge of thermal tolerance limits, but also effects of temperature change on growth rates and other life history parameters. For arthropods that exhibit discontinuous growth (i.e., molting), this includes both intermolt period and growth per molt. Previous laboratory and field experiments suggest that temperature affects both intermolt period (IMP) and growth per …


Combined Effects Of Dissolved Oxygen And Temperature On Aerobic Respiration And Respiratory Recovery Responses Of The Spioniform Polychaete, Streblospio Gynobranchiata, In Relation To Body Size, Alyssa Bennett Dec 2017

Combined Effects Of Dissolved Oxygen And Temperature On Aerobic Respiration And Respiratory Recovery Responses Of The Spioniform Polychaete, Streblospio Gynobranchiata, In Relation To Body Size, Alyssa Bennett

Master's Theses

Elevated surface temperatures exacerbate the threat of hypoxia within coastal ecosystems. These two primary stressors likely interact as they elicit opposing physiological responses from marine organisms. Metabolic depression is typically associated with hypoxia, while metabolic rates increase with temperature. Moreover, physiological effects of combined stressors may not be additive. In light of increasing pressures from hypoxia, elevated ocean temperatures, and other stressors within coastal regions, studies need to examine effects of multiple stressors on physiology of coastal organisms.

Mass-specific aerobic respiration (VO2) was characterized as a proxy for metabolic cost of Streblospio gynobranchiata, at combined levels of …


Proteomic Analysis Of The Crustacean Molting Gland (Y-Organ) Over The Course Of The Molt Cycle, Talia B. Head Sep 2017

Proteomic Analysis Of The Crustacean Molting Gland (Y-Organ) Over The Course Of The Molt Cycle, Talia B. Head

Master's Theses

Molting in crustaceans is a highly complex physiological process involving negative regulation by two paired endocrine glands, the X-organ/sinus gland complex (XO/SG) and the Y-organ (YO). The XO/SG complex is responsible for making molt-inhibiting hormone (MIH) which negatively regulates synthesis of the molting hormones, ecdysteroids, by the YO. Analysis of gene expression in the XOs and YOs has led to the development of a proposed molecular signaling pathway which regulates ecdysteroidogenesis and subsequent molting in crustaceans. In this study, changes in protein abundance in the YO were characterized over the course of a molt cycle (intermolt, early premolt, mid premolt, …


Effects Of Ankle Weights On Metabolic Response And Muscle Activity On A Lower Body Positive Pressure Treadmill 2017, Saige Hupman May 2017

Effects Of Ankle Weights On Metabolic Response And Muscle Activity On A Lower Body Positive Pressure Treadmill 2017, Saige Hupman

Master's Theses

Lower body positive pressure (LBPP) treadmills are growing in popularity for rehabilitative use, as the benefits of exercising at partially supported body weight may induce faster recovery. It is unknown if there are certain practices that increase exercise intensity while maintaining positive effects of LBPP. Adding ankle weights when walking or running could increase intensity of rehabilitation programs while maintaining the comfort of supported body weight. PURPOSE: To measure metabolic response (VO2, RER, HR, Caloric expenditure), RPE, and lower limb electromyography (EMG) amplitudes of LBPP treadmill walking and running with and without ankle weights. METHODS: Sixteen participants (Age: 21.94 ± …


The Effects Of Quadmill Training On Balance: An Intervention Study 2017, Alexander J. Generali May 2017

The Effects Of Quadmill Training On Balance: An Intervention Study 2017, Alexander J. Generali

Master's Theses

The purpose of this study was to observe and compare the effects of two separate training interventions using the QuadmillTM to a control group on balance in college-aged individuals. It was hypothesized that both experimental groups (I1 and I2) would experience improvements in balance over the intervention whereas the control group would remain at baseline. It was secondarily hypothesized that I2 would experience greater improvements than I1 in balance due to the nature of the exercise protocol. Data was collected using three systems; Balance Tracking Systems, Star Excursion Balance Test, and the Biodex Balance system. A two-way mixed methods ANOVA …


The Effect Of Blood Flow Restriction Training With The Quadmill On Peak Isometric Knee Extensor Strength 2017, Brandon Schrom May 2017

The Effect Of Blood Flow Restriction Training With The Quadmill On Peak Isometric Knee Extensor Strength 2017, Brandon Schrom

Master's Theses

Traditional training methodologies that improve muscular strength use loads as low as 75% of a person’s one-repetition maximum and as high as 110% of a person’s one repetition maximum. With these high loads comes a greater risk for injury. Blood flow restriction (BFR) training is a potential solution to this problem. BFR training originated in Japan, where it was called Kaatsu. With this method of training, a trainee ties a tourniquet around the proximal end of a limb to reduce blood flow to and from the limb’s muscles. The purpose of this study was to determine whether three-weeks of BFR …


Imposed Versus Affect-Based Resistance Training Intensities On Adherence, Session Affect, Session Perceived Exertion, And Intrinsic Motivation Measures During A Six-Week Program In Novice Female Lifters 2017, Ryan Joseph Brennan May 2017

Imposed Versus Affect-Based Resistance Training Intensities On Adherence, Session Affect, Session Perceived Exertion, And Intrinsic Motivation Measures During A Six-Week Program In Novice Female Lifters 2017, Ryan Joseph Brennan

Master's Theses

The American College of Sports Medicine (ACSM) recommends that individuals perform resistance training exercises at a specific intensity based on external load (percentage of one repetition maximum; %1RM). However, only 29.6% of adults in 2013 reported strength training two or more times per week. Furthermore, individuals lifting at recommended percentages vary in their pleasure and displeasure. Self-selected exercise seems to promote positive affective responses, in part, due to the perceived autonomy. The effects of regulating exercise intensity using affect as opposed to imposed intensities as a means for improved fitness, promoting exercise behavior, and enhancing other psychological outcomes have yet …


The Relationship Between Ratings Of Perceived Exertion And Heart Rate In Ncaa Division Iii Male Soccer Players 2017, Samuel J. Guider May 2017

The Relationship Between Ratings Of Perceived Exertion And Heart Rate In Ncaa Division Iii Male Soccer Players 2017, Samuel J. Guider

Master's Theses

Every sport has specific physical demands of the human body. The amount of physiological load that an athlete endures during a bout of exercise can be described as training load (TL). Accurate calculation of training loads within athletes is important when it comes to strength and power development, as well as injury prevention and monitoring fatigue. Common methods used to calculate training loads for athletes include rating of perceived exertion (RPE) based methods, heart rate (HR) based methods, rate of oxygen consumption (VO2) methods, and blood lactate methods. Specifically with NCAA male soccer athletes, HR based methods and RPE based …


Characterization Of Andrenocortical Tissue Morphology, Histology, And Steroid Synthesis Among Finetooth (Carcharhinus Isodon), Blacktip (Carcharhinus Limbatus), Atlantic Sharpnose (Rhizoprionodon Terraenovae), And Bonnethead (Sphyrna Tiburo) Sharks, Danielle Elizabeth Bailey May 2017

Characterization Of Andrenocortical Tissue Morphology, Histology, And Steroid Synthesis Among Finetooth (Carcharhinus Isodon), Blacktip (Carcharhinus Limbatus), Atlantic Sharpnose (Rhizoprionodon Terraenovae), And Bonnethead (Sphyrna Tiburo) Sharks, Danielle Elizabeth Bailey

Master's Theses

Adrenocortical (interrenal) tissue is composed of steroidogenic cells that produce corticosteroids involved in the stress response and hydromineral balance. Previous research characterizing the elasmobranch interrenal suggests that the number of interrenal bodies that produce the single primary corticosteroid, 1a-hydroxycorticosterone (1a-OHB), varies among species. However, potential species-specific differences in the amount of interrenal tissue and major steroid products produced have been understudied. To address this critical gap in our understanding of elasmobranch biology, this study examined interrenal morphology, cell structure, steroidogenic enzyme distribution (3bHSD), and steroid production in four shark species: Finetooth Carcharhinus isodon, Blacktip Carcharhinus limbatus, Atlantic Sharpnose …


The Insulin-Like Growth Factor-1 (Igf1) System As A Potential Biomarker For Nutritional Status And Growth Rate In Pacific Rockfish (Sebastes Spp.), Nicole L. Hack Mar 2017

The Insulin-Like Growth Factor-1 (Igf1) System As A Potential Biomarker For Nutritional Status And Growth Rate In Pacific Rockfish (Sebastes Spp.), Nicole L. Hack

Master's Theses

Growth performance in vertebrates is regulated by environmental factors including the quality and quantity of food, which influences growth via endocrine pathways such as the growth hormone (GH) / insulin-like growth factor somatotropic axis. In several teleost fishes, circulating concentrations of insulin-like growth factor-1 (Igf1) correlate positively with growth rate, and it has been proposed that plasma Igf1 levels may serve as an indicator of growth variation for fisheries and aquaculture applications. Here, I tested whether plasma Igf1 concentrations might serve as an indicator of somatic growth in olive rockfish (Sebastes serranoides), one species among dozens of rockfishes …


The Validity Of Height As A Determinant Of Takeoff Distance To Hurdle One In Ncaa Division Iii Hurdlers 2017, Phillip Wiltshire Jan 2017

The Validity Of Height As A Determinant Of Takeoff Distance To Hurdle One In Ncaa Division Iii Hurdlers 2017, Phillip Wiltshire

Master's Theses

The purpose of this study was to determine if the relationships between the height of a hurdler and the takeoff distance to the first hurdle for men and women as reported by Frye (2008) were valid for NCAA Division III 100 m and 110 m hurdlers. With no specific research reported to validate Frye’s data, it is unclear how the suggested takeoff distances were developed. It is also unclear whether hurdler height or another factor is the main determinant of takeoff distance for the high hurdlers. Participants in this study were three female 100 m hurdlers and four male 110 …


Araucaria In The Urban Landscape: A Novel Leaning Pattern And Evidence Of Cultivated Hybridization, Jason W. Johns Jan 2017

Araucaria In The Urban Landscape: A Novel Leaning Pattern And Evidence Of Cultivated Hybridization, Jason W. Johns

Master's Theses

Our understanding of the natural world is constantly evolving and strengthening as more observations are made and experiments are performed. For example, we understand that tree stems grow toward the light (positive phototropism; Darwin 1880, Loehle 1986, Christie et al. 2013) and against gravity (negative gravitropism; Knight 1806, Hashiguchi et al. 2013). We also know that plants respond to mechanical stimulus and perturbation (thigmotropism; Braam 2005). Genes and their resulting proteins have been described to uncover some of the mechanisms for these environmental responses, but relatively speaking, we have just scratched the surface (Wyatt et al. 2013). While the discovery …


Functional Studies Of Novel Mosquito Stage-Specific Genes In The Malaria Parasite, Plasmodium Berghei., Kaitlyn Kiernan Jan 2017

Functional Studies Of Novel Mosquito Stage-Specific Genes In The Malaria Parasite, Plasmodium Berghei., Kaitlyn Kiernan

Master's Theses

The development and survival of the malaria parasite Plasmodium within its mosquito vector is not very well understood. When the parasite is taken up by an Anopheles mosquito, it is exposed to digestive enzymes, reactive oxygen species, increasing pH, decreasing temperature, and a diverse microbial flora. Our lab is studying the adaptive mechanisms underlying parasite survival in this hostile environment, one of which is movement out of the midgut. In order to escape the midgut, the parasite develops into an elongated, motile form called the ookinete. I am investigating two genes, thioredoxin-like protein 1 (trxl-1) and subpellicular microtubule protein 1 …


The Elasmobranch-Microbe Relationship: Trimethylamine N-Oxide Synthesis, Urea Hydrolysis, And Microbe-Osmolyte Interactions In The Atlantic Stingray, Dasyatis Sabina, Kaitlin Kelly Doucette Dec 2016

The Elasmobranch-Microbe Relationship: Trimethylamine N-Oxide Synthesis, Urea Hydrolysis, And Microbe-Osmolyte Interactions In The Atlantic Stingray, Dasyatis Sabina, Kaitlin Kelly Doucette

Master's Theses

The elasmobranch osmoregulatory strategy is predicated on the accumulation of nitrogenous compounds, primarily urea and trimethylamine n¬-oxide (TMAO). Despite the abundance of these plasma osmolytes, it is notable that elasmobranchs appear to lack urease and TMA oxidase (Tmase), enzymes that scavenge urea-nitrogen and synthesize TMAO, respectively. However, urease and Tmase are found in many species of bacteria. Therefore, I hypothesized that intestinal bacteria are responsible for urease and Tmase activity in elasmobranchs. Absent dietary nitrogen sources, I evaluated the effects of reduced intestinal microbiota on osmoregulation in Atlantic stingray (Dasyatis sabina) in vivo. D. sabina were given …


Effect Of Oxygen-Limiting Tidal Conditions On Muscle Metabolism And Structure In The Giant Acorn Barnacle, Balanus Nubilus, Katie O. Grady Dec 2016

Effect Of Oxygen-Limiting Tidal Conditions On Muscle Metabolism And Structure In The Giant Acorn Barnacle, Balanus Nubilus, Katie O. Grady

Master's Theses

Crustacean muscle fibers are some of the largest cells in the animal kingdom, with fiber diameters in the giant acorn barnacle (Balanus nubilus) exceeding 3 mm. Sessile animals with extreme muscle sizes and that live in the hypoxia-inducing intertidal zone – like B. nubilus – represent ideal models for probing the effects of oxygen limitation on muscle cells. We investigated changes in metabolism and structure of B. nubilus muscle in response to: normoxic immersion, anoxic immersion, or air emersion, for acute (6h) or chronic (6h exposures twice daily for 2wks) time periods. Following exposure, we immediately measured hemolymph …


The Proteomic Response Of Gill Tissue In Tidally And Subtidally-Acclimated California Mussels, Mytilus Californianus, To Acute Emersion-Induced Anoxia, Aubrie N. Fowler, Lars Tomanek Aug 2016

The Proteomic Response Of Gill Tissue In Tidally And Subtidally-Acclimated California Mussels, Mytilus Californianus, To Acute Emersion-Induced Anoxia, Aubrie N. Fowler, Lars Tomanek

Master's Theses

Intertidal mussels regularly experience emersion-induced anoxia, in contrast to normoxic conditions experienced during submersion. We therefore hypothesized that acclimation to a tidal rhythm, as opposed to a rhythm of constant submersion, preconditions the proteome of the California mussel, Mytilus californianus, to respond differently to emersion-induced anoxia. Following acclimation, mussels either continued to receive the acclimation conditions (control) or were exposed to 100% nitrogengas (anoxia) during aerial emersion. We collected gill tissue for subsequent analysis of protein abundance with 2D gel electrophoresis and protein identification with tandem mass spectrometry. Relative to subtidally-acclimated mussels, tidally-acclimated mussels showed a greater propensity to …