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Articles 901 - 930 of 1180

Full-Text Articles in Pharmacology

Nutritional Composition In The Chia Seed And Its Processing Properties On Restructured Ham-Like Products, Y. Ding, H.-W. Lin, Y.-L. Lin, D.-J. Yang, Y.-S. Yu, J.-W. Chen, S.-Y. Wang, Y.-C. Chen Jul 2020

Nutritional Composition In The Chia Seed And Its Processing Properties On Restructured Ham-Like Products, Y. Ding, H.-W. Lin, Y.-L. Lin, D.-J. Yang, Y.-S. Yu, J.-W. Chen, S.-Y. Wang, Y.-C. Chen

Journal of Food and Drug Analysis

Low-fat meat products always have harder texture, lower juiciness, and worse flavor. Due to their higher water-holding, water absorption, and organic molecule absorption, chia seeds (CHIA) have been applied in powders, nutrition bars, breads, and cookies. Hence, the objectives of this study were to: (1) analyze the nutritional compositions in CHIA; and (2) look for the possible application of CHIA on restructured ham-like products. CHIA has high amounts of α-linolenic acid, crude polysaccharides, and also contains essential amino acids, minerals, and polyphenols. Regarding processing properties of CHIA, a combination of CHIA and carrageenan (CA) increased (p < 0.05) production yield of restructured ham-like products. A scanning electron microscope observation indicated that CHIA and CA addition can assist an emulsification in this ham-like product. Addition of 0.5% CA and 1.0% CHIA in this ham-like product showed the similar overall acceptance as products with added fat. Following storage at 4°C, higher (p < 0.05) purge and centrifugation losses, as well as hardness of this ham-like product can be improved by adding CHIA and CA. CHIA addition also resulted in lower (p < 0.05) lipid and protein oxidation, especially a 1.0% addition. In summary, due to both nutritional addition and improvements on physicochemical and sensorial properties of restructured ham-like products, CHIA seeds have great potential on the development of healthy and good-quality meat products. © 2017

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Hypocholesterolemic Activity Of Indigenous Probiotic Isolate Saccharomyces Cerevisiae Ardmc1 In A Rat Model, D. Saikia, A.K. Manhar, B. Deka, R. Roy, K. Gupta, N.D. Namsa, P. Chattopadhyay, R. Doley, M. Mandal Jul 2020

Hypocholesterolemic Activity Of Indigenous Probiotic Isolate Saccharomyces Cerevisiae Ardmc1 In A Rat Model, D. Saikia, A.K. Manhar, B. Deka, R. Roy, K. Gupta, N.D. Namsa, P. Chattopadhyay, R. Doley, M. Mandal

Journal of Food and Drug Analysis

The aim of this study was to investigate probiotic attributes of Saccharomyces cerevisiae ARDMC1 isolated from traditional rice beer starter cake and its hypocholesterolemic effects on Wistar rats fed a high-cholesterol diet. The indigenous isolate ARDMC1 showed potential probiotic characteristics such as tolerance to simulated gastrointestinal stress conditions, autoaggregation properties, and adhesion to intestinal epithelium Caco-2 cell line. In addition, ARDMC1 isolate exhibited in vitro cholesterol assimilation properties in media supplemented with cholesterol. Furthermore, administration of probiotic isolate to rats fed a hypercholesterolemic diet resulted in significant reduction of serum total cholesterol, low-density lipoprotein cholesterol, and triglyceride at the end …

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Ceratonia Siliqua Honeys From Morocco: Physicochemical Properties, Mineral Contents, And Antioxidant Activities, R. El-Haskoury, W. Kriaa, B. Lyoussi, M. Makni Jul 2020

Ceratonia Siliqua Honeys From Morocco: Physicochemical Properties, Mineral Contents, And Antioxidant Activities, R. El-Haskoury, W. Kriaa, B. Lyoussi, M. Makni

Journal of Food and Drug Analysis

Physicochemical properties, main mineral content, and antioxidant activity were determined for eight floral carob honeys collected from different geographical regions of Morocco. Moroccan honeys showed good chemical and nutritional qualities, fulfilling the criteria described in the standard codex for honey. The percentages obtained for ashes were (0.13–0.69%), electrical conductivity (0.36–1.35 mS/cm), water content (17.30–22.80%), pH (4.17–5.05), free acidity (11.0–42.50 meq/kg), lactone acidity (4.0–16.50 meq/kg), and for total acidity (16.50–59.50 meq/kg). In addition, minerals such as K, Na, Mg, Cu, Zn, and Ca of honey samples were determined and potassium was the major mineral in all samples. The antioxidant activities based …

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Urine Specimen Validity Test For Drug Abuse Testing In Workplace And Court Settings, S.-Y. Lin, H.-H. Lee, J.-F. Lee, B.-H. Chen Jul 2020

Urine Specimen Validity Test For Drug Abuse Testing In Workplace And Court Settings, S.-Y. Lin, H.-H. Lee, J.-F. Lee, B.-H. Chen

Journal of Food and Drug Analysis

In recent decades, urine drug testing in the workplace has become common in many countries in the world. There have been several studies concerning the use of the urine specimen validity test (SVT) for drug abuse testing administered in the workplace. However, very little data exists concerning the urine SVT on drug abuse tests from court specimens, including dilute, substituted, adulterated, and invalid tests. We investigated 21,696 submitted urine drug test samples for SVT from workplace and court settings in southern Taiwan over 5 years. All immunoassay screen-positive urine specimen drug tests were confirmed by gas chromatography/mass spectrometry. We found …

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Convenient Isolation Of Strictinin-Rich Tea Polyphenol From Chinese Green Tea Extract By Zirconium Phosphate, Y. Ma, Y. Shang, F. Liu, W. Zhang, C. Wang, D. Zhu Jul 2020

Convenient Isolation Of Strictinin-Rich Tea Polyphenol From Chinese Green Tea Extract By Zirconium Phosphate, Y. Ma, Y. Shang, F. Liu, W. Zhang, C. Wang, D. Zhu

Journal of Food and Drug Analysis

Zirconium phosphate (ZrP) was prepared and employed to separate strictinin-rich tea polyphenol from Chinese green tea extracts. The influences of ZrP calcination temperatures, green tea extraction conditions, and the amounts of ZrP on the isolation of strictinin-rich tea polyphenol were evaluated; the absorption and desorption dynamics of strictinin on ZrP were also determined. Our results revealed that the HPLC content of strictinin increased from 4.96% in 70% ethanol extract of green tea to 58.2% in isolated strictinin-rich tea polyphenol obtained by ZrP-900 (ZrP calcined at 900°C). Furthermore, the suitable time for both strictinin absorption and desorption was 4 hours at …

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Functional Components In Scutellaria Barbata D. Don With Anti-Inflammatory Activity On Raw 264.7 Cells, H.-L. Liu, T.-H. Kao, C.-Y. Shiau, B.-H. Chen Jul 2020

Functional Components In Scutellaria Barbata D. Don With Anti-Inflammatory Activity On Raw 264.7 Cells, H.-L. Liu, T.-H. Kao, C.-Y. Shiau, B.-H. Chen

Journal of Food and Drug Analysis

The objectives of this study were to determine the variety and amount of various functional components in Scutellaria barbata D. Don as well as study their anti-inflammatory activity on RAW 264.7 cells. Both ethanol and ethyl acetate extracts were shown to contain the functional components including phenolics, flavonoids, chlorophylls, and carotenoids, with the former mainly composed of phenolics and flavonoids, and the latter of carotenoids and chlorophylls. Both extracts could significantly inhibit (p < 0.05) the production of lipopolysaccharide-induced nitric oxide, prostaglandin E 2 , interlukin-6, and interlukin-1β, as well as the expressions of phosphor extracellular signal-regulated kinase and phosphor-c-Jun N-terminal kinase (p-JNK), but failed to retard tumor necrosis factor-α expression. Both ethanol and ethyl acetate extracts had a dose-dependent anti-inflammatory activity on RAW 264.7 cells. Furthermore, the anti-inflammatory efficiency can be varied for both ethanol and ethyl acetate extracts, which can be attributed to the presence of different varieties and amounts of functional components, as mentioned above. This finding suggested that S. Barbata extract may be used as an anti-inflammatory agent for possible future biomedical application. © 2017

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Designing A Biochip Following Multiplex Polymerase Chain Reaction For The Detection Of Salmonella Serovars Typhimurium, Enteritidis, Infantis, Hadar, And Virchow In Poultry Products, Y.-C. Chiang, H.-H. Wang, L. Ramireddy, H.-Y. Chen, C.-M. Shih, C.-K. Lin, H.-Y. Tsen Jul 2020

Designing A Biochip Following Multiplex Polymerase Chain Reaction For The Detection Of Salmonella Serovars Typhimurium, Enteritidis, Infantis, Hadar, And Virchow In Poultry Products, Y.-C. Chiang, H.-H. Wang, L. Ramireddy, H.-Y. Chen, C.-M. Shih, C.-K. Lin, H.-Y. Tsen

Journal of Food and Drug Analysis

Salmonella-contaminated foods, especially poultry-derived foods (eggs, chicken meat), are the major source of salmonellosis. Not only in the European Union (EU), but also in the United States, Japan, and other countries, has salmonellosis been an issue of concern for food safety control agencies. In 2005, EU regulation 1003/2005 set a target for the control and reduction of five target Salmonella enterica serovars—S. Typhimurium, S. Enteritidis, S. Infantis, S. Hadar, and S. Virchow—in breeding flocks. Thus, a simple biochip for the rapid detection of any of these five Salmonella serovars in poultry products may be required. The objectives of this study …

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Tea Silkworm Droppings As An Enriched Source Of Tea Flavonoids, T.-Y. Chou, M.-J. Yang, S.-K. Tseng, S.-S. Lee, C.-C. Chang Jul 2020

Tea Silkworm Droppings As An Enriched Source Of Tea Flavonoids, T.-Y. Chou, M.-J. Yang, S.-K. Tseng, S.-S. Lee, C.-C. Chang

Journal of Food and Drug Analysis

Andraca droppings is the waste excreted from the tea biter Andraca theae. Its chemical constituents and potential medical use, unlike those of the traditional Chinese medicine silkworm droppings, have not been reported yet. To explore new nutraceuticals, the chemical constituents of this substance were investigated. Since the bioactive ingredients are generally present in the EtOAc-soluble fraction, this fraction, obtained from the ethanolic extract of the dried Andraca droppings by liquid–liquid partitioning, was separated by chromatographic methods, including Sephadex LH-20, centrifugal partition chromatography, and RP-18 columns, to produce 14 compounds (1–14). They were characterized as 1,7-dimethyl xanthine (1), three benzoic acids …

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Vegetable, Fruit, And Phytonutrient Consumption Patterns In Taiwan, W.-H. Pan, N.-H. Yeh, R.-Y. Yang, W.-H. Lin, W.-C. Wu, W.-T. Yeh, M.-K. Sung, H.-S. Lee, S.-J. Chang, C.-J. Huang, B.-F. Lin, M.-T. Chiang Jul 2020

Vegetable, Fruit, And Phytonutrient Consumption Patterns In Taiwan, W.-H. Pan, N.-H. Yeh, R.-Y. Yang, W.-H. Lin, W.-C. Wu, W.-T. Yeh, M.-K. Sung, H.-S. Lee, S.-J. Chang, C.-J. Huang, B.-F. Lin, M.-T. Chiang

Journal of Food and Drug Analysis

Phytonutrients may play important roles in human health and yet only recently a few studies have described phytonutrient consumption patterns, using data obtained from daily consumption methods. We aimed to estimate the phytonutrient content in Taiwanese diets and analyzed main food sources of 10 major phytonutrients. In this study, food items and dietary data gathered with the 24-hour dietary recall from 2908 participants in the 2005–2008 Nutrition and Health Survey in Taiwan were used to create a food phytonutrient database with 933 plant-based foods through integrating database, literature search, and chemical analysis and to appraise phytonutrient consumption status of participants. …

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Antioxidant Activity And Anticancer Effect Of Ethanolic And Aqueous Extracts Of The Roots Of Ficus Beecheyana And Their Phenolic Components, G.-C. Yen, C.-S. Chen, W.-T. Chang, M.-F. Wu, F.-T. Cheng, D.-K. Shiau, C.-L. Hsu Jul 2020

Antioxidant Activity And Anticancer Effect Of Ethanolic And Aqueous Extracts Of The Roots Of Ficus Beecheyana And Their Phenolic Components, G.-C. Yen, C.-S. Chen, W.-T. Chang, M.-F. Wu, F.-T. Cheng, D.-K. Shiau, C.-L. Hsu

Journal of Food and Drug Analysis

This study aimed to investigate the antioxidant and anticancer effects of ethanolic and aqueous extracts of the roots of Ficus beecheyana (EERFB and AERFB) and their phenolic components. In this study, total phenolic content and antioxidant activity of EERFB were higher than those of AERFB. Major phenolic compounds in the extracts were gallic acid, p-hydroxybenzoic acid, caffeic acid, chlorogenic acid, p-coumaric acid, and rutin; which were identified by high-performance liquid chromatography. Flow cytometric analysis of HL-60 cells exposed to EERFB showed that the percentage of apoptotic cells increased in a dose-dependent manner. EERFB treatment resulted in the loss of mitochondrial …

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Protective Effect Of Nelumbo Nucifera Extracts On Beta Amyloid Protein Induced Apoptosis In Pc12 Cells, In Vitro Model Of Alzheimer's Disease, A. Kumaran, C.C. Ho, L.S. Hwang Jul 2020

Protective Effect Of Nelumbo Nucifera Extracts On Beta Amyloid Protein Induced Apoptosis In Pc12 Cells, In Vitro Model Of Alzheimer's Disease, A. Kumaran, C.C. Ho, L.S. Hwang

Journal of Food and Drug Analysis

Alzheimer's disease (AD) is the most common cause of dementia in the elderly. β-Amyloid (Aβ) has been proposed to play a role in the pathogenesis of AD. Deposits of insoluble Aβ are found in the brains of patients with AD and are one of the pathological hallmarks of the disease, but the underlying signaling pathways are poorly understood. In order to develop antidementia agents with potential therapeutic value, we examined the inhibitory effect of the Nelumbo nucifera seed embryo extracts on to the aggregated amyloid β peptide (agg Aβ 1–40 )-induced damage of differentiated PC-12 cells (dPC-12), a well-known cell …

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Effects Of Lemongrass Oil And Citral On Hepatic Drug-Metabolizing Enzymes, Oxidative Stress, And Acetaminophen Toxicity In Rats, C.-C. Li, H.-F. Yu, C.-H. Chang, Y.-T. Liu, H.-T. Yao Jul 2020

Effects Of Lemongrass Oil And Citral On Hepatic Drug-Metabolizing Enzymes, Oxidative Stress, And Acetaminophen Toxicity In Rats, C.-C. Li, H.-F. Yu, C.-H. Chang, Y.-T. Liu, H.-T. Yao

Journal of Food and Drug Analysis

The essential oil from a lemongrass variety of Cymbopogon flexuosus [lemongrass oil (LO)] is used in various food and aroma industry products and exhibits biological activities, such as anticancer and antimicrobial activities. To investigate the effects of 200 LO (200 mg/kg) and 400 LO (400 mg/kg) and its major component, citral (240 mg/kg), on drug-metabolizing enzymes, oxidative stress, and acetaminophen toxicity in the liver, male Sprague-Dawley rats were fed a pelleted diet and administered LO or citral by gavage for 2 weeks. After 2 weeks of feeding, the effects of LO and citral on the metabolism and toxicity of acetaminophen …

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The Effects Of Ficus Carica On The Activity Of Enzymes Related To Metabolic Syndrome, R. Mopuri, M. Ganjayi, B. Meriga, N.A. Koorbanally, M.S. Islam Jul 2020

The Effects Of Ficus Carica On The Activity Of Enzymes Related To Metabolic Syndrome, R. Mopuri, M. Ganjayi, B. Meriga, N.A. Koorbanally, M.S. Islam

Journal of Food and Drug Analysis

The present study aimed to investigate the effects of the various parts of Ficus carica L. (figs) on antioxidant, antidiabetic, and antiobesogenic effects in vitro. Fruit, leaves, and stembark of the F. carica plant were sequentially extracted using organic and inorganic solvents and their total polyphenol and flavonoid contents were estimated. The effects of the extracts on antioxidative, antidiabetic (inhibition of α-amylase and α-glucosidase enzymes), and antiobesogenic (antilipase) activities were measured using several experimental models. The fruit ethanolic extract contained a high quantity of polyphenols and flavonoids (104.67 ± 5.51 μg/mL and 81.67 ± 4.00 μg/mL) compared with all other …

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Extended Aroma Extract Dilution Analysis Profile Of Shiikuwasha (Citrus Depressa Hayata) Pulp Essential Oil, Y. Asikin, S. Kawahira, M. Goki, N. Hirose, S. Kyoda, K. Wada Jul 2020

Extended Aroma Extract Dilution Analysis Profile Of Shiikuwasha (Citrus Depressa Hayata) Pulp Essential Oil, Y. Asikin, S. Kawahira, M. Goki, N. Hirose, S. Kyoda, K. Wada

Journal of Food and Drug Analysis

Shiikuwasha pulp is an important raw material for producing citrus essential oils. The volatile aroma composition of pulp essential oil was evaluated using gas chromatography (GC) methods, and its aroma profile was assessed using GC-olfactometry with an extended aroma extract dilution analysis (AEDA) technique in regard to alterations of odor strength and sensorial perception throughout serial dilution steps. The essential oil comprised a mixture of 55 aroma compounds, including monoterpene hydrocarbon, sesquiterpene hydrocarbon, alcohol, aldehyde, ester, and oxide compounds. The predominant compounds were limonene [57.36% (4462.80 mg/100 g of pulp)] and γ-terpinene [25.14% (1956.21 mg/100 g of pulp)]. However, linalool …

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Determination Of Carnosic Acid In Rosmarinus Officinalis L. Using Square Wave Voltammetry And Electrochemical Behavior, Ümmihan Taşkoparan Yilmaz, E. Calik, B. Akdulum, H. Yilmaz Jul 2020

Determination Of Carnosic Acid In Rosmarinus Officinalis L. Using Square Wave Voltammetry And Electrochemical Behavior, Ümmihan Taşkoparan Yilmaz, E. Calik, B. Akdulum, H. Yilmaz

Journal of Food and Drug Analysis

A new, fast, sensitive and simple voltammetric method is established for the direct determination of carnosic acid (CA). And the electroreduction of carnosic acid (CA) was studied using electrochemical methods. The number of electrons transferred in electrode mechanisms were calculated for reversible and adsorption-controlled electrochemical reduction of CA at 17 mV versus Ag/AgCl at pH 7.0 in Britton–Robinson buffer (BR) on a hanging mercury drop electrode. Square-wave voltammetry was developed and validated for direct determination of CA. Square-wave parameters were optimized as accumulation potential = 0.0 mV, accumulation time = 5 s, frequency = 50 Hz, pulse amplitude = 50 …

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The Apple Polyphenol Phloretin Inhibits Breast Cancer Cell Migration And Proliferation Via Inhibition Of Signals By Type 2 Glucose Transporter, K.-H. Wu, C.-T. Ho, Z.-F. Chen, L.-C. Chen, J. Whang-Peng, T.-N. Lin, Y.-S. Ho Jul 2020

The Apple Polyphenol Phloretin Inhibits Breast Cancer Cell Migration And Proliferation Via Inhibition Of Signals By Type 2 Glucose Transporter, K.-H. Wu, C.-T. Ho, Z.-F. Chen, L.-C. Chen, J. Whang-Peng, T.-N. Lin, Y.-S. Ho

Journal of Food and Drug Analysis

Human triple-negative breast cancer (TNBC) is the most aggressive and poorly understood subclass of breast cancer. Glucose transporters (GLUTs) are required for glucose uptake in malignant cancer cells and are ideal targets for cancer therapy. To determine whether the inhibition of GLUTs could be used in TNBC cell therapy, the apple polyphenol phloretin (Ph) was used as a specific antagonist of GLUT2 protein function in human TNBC cells. Interestingly, we found that Ph (10–150 μM, for 24 h) inhibited cell growth and arrested the cell cycle in MDA-MB-231 cells in a p53 mutant-dependent manner, which was confirmed by pre-treatment of …

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The Inhibition Of Advanced Glycation End-Products By Five Fractions And Three Main Flavonoids From Camellia Nitidissima Chi Flowers, R. Yang, W.-X. Wang, H.-J. Chen, Z.-C. He, A.-Q. Jia Jul 2020

The Inhibition Of Advanced Glycation End-Products By Five Fractions And Three Main Flavonoids From Camellia Nitidissima Chi Flowers, R. Yang, W.-X. Wang, H.-J. Chen, Z.-C. He, A.-Q. Jia

Journal of Food and Drug Analysis

Camellia nitidissima Chi (CNC), belonging to Camellia genus (Theaceae family), is a medicinal and edible plant in China. Among the whole plant, the CNC flowers are especially precious, but the biological activities and the compositions of the CNC flowers are unknown. In this study, inhibiting effects on the formation of advanced glycation end-products (AGEs) of five CNC flowers fractions and three isolated compounds were investigated, these three compounds are two flavonoid glycosides and one flavanol, namely kaempferol 3-O-[2,3,4-Tri-O-acetyl-α-L-rhamnopyranosyl-(1→3)-2,4-di-O-acetyl-α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside, kaempferol 3-O-[2,3,4-Tri-O-acetyl-α-L-rhamnopyranosyl-(1→3)-4-O-acetyl-α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside and catechin. Among these five fractions, the ethyl acetate fraction showed the highest total phenolic contents and inhibiting effects on …

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Application Of Corona Electrical Discharge Plasma On Modifying The Physicochemical Properties Of Banana Starch Indigenous To Taiwan, T.-Y. Wu, N.-N. Sun, C.-F. Chau Jul 2020

Application Of Corona Electrical Discharge Plasma On Modifying The Physicochemical Properties Of Banana Starch Indigenous To Taiwan, T.-Y. Wu, N.-N. Sun, C.-F. Chau

Journal of Food and Drug Analysis

Corona electrical discharge (CED) belongs to an atmospheric pressure cold plasma. In this study, raw banana starch (indigenous to Taiwan), which contained resistant starch and amylose at a level of 58.4 g/100 g and 14.5 g/100 g, respectively, was treated by CED at 30 kV/cm, 40 kV/cm, and 50 kV/cm for 3 minutes. After the CED treatment, starch analyses showed that there were no apparent changes in the resistant starch and amylose contents. Only surface and nonpenetrative damage caused by plasma etching at different voltage strengths were observed on the starch granules. The CED treatments reduced the total area of …

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Metabolic Profiling Investigation Of Fritillaria Thunbergii Miq. By Gas Chromatography–Mass Spectrometry, M.-C. Cui, S.-J. Chen, H.-H. Wang, Z.-H. Li, H.-J. Chen, Y. Chen, H.-B. Zhou, X. Li, J.-W. Chen Jul 2020

Metabolic Profiling Investigation Of Fritillaria Thunbergii Miq. By Gas Chromatography–Mass Spectrometry, M.-C. Cui, S.-J. Chen, H.-H. Wang, Z.-H. Li, H.-J. Chen, Y. Chen, H.-B. Zhou, X. Li, J.-W. Chen

Journal of Food and Drug Analysis

Thunberg fritillary bulb (the dry bulbs of Fritillaria thunbergii Miq.), a traditional Chinese Medicine, is widely applied as an expectorant and antitussive. In this investigation, the primary metabolites of bulbs, flowers, leaves, and stems of F. thunbergii were analyzed by gas chromatography–mass spectrometry. Principal component analysis, partial least squares-discriminate analysis, orthogonal projection to latent structures-discriminate analysis, and heat map analysis showed that there were dissimilar metabolites, and a negative correlation between amino acids and saccharides in different analytes. Furthermore, carbodiimide, tryptophan, glucose-6-phosphate, xylose, 2-piperidinecarboxylic acid, monoamidomalonic acid, phenylalanine, and histidine were found to play an important role in the plant …

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Simultaneous Determination Of Four Amides In Saururus Chinensis By Matrix Solid Phase Dispersion And High-Performance Liquid Chromatography Method, H. Chen, J. Liu, M. Cui, J. Chen, X. Li, Y. Chen Jul 2020

Simultaneous Determination Of Four Amides In Saururus Chinensis By Matrix Solid Phase Dispersion And High-Performance Liquid Chromatography Method, H. Chen, J. Liu, M. Cui, J. Chen, X. Li, Y. Chen

Journal of Food and Drug Analysis

A rapid and simple analytical method was established for the determination of four amides (N-p-trans-coumaroyltyramine, aristolactam AⅡ, sauristolactam and aristolactam BⅡ) in Saururus chinensis by matrix solid phase dispersion (MSPD) and high-performance liquid chromatography-diode array detector (HPLC-DAD). In the optimized MSPD, 0.2 g S. chinensis powder was blended with 0.4 g silica gel, and 5 mL methanol was selected as elution solvent. The MSPD extraction achieved higher extraction recovery of four amides, and required less sample, solvent and preparation time, comparing with the conventional methods (Soxhlet and ultrasonic extraction). The assay was performed on a TSK gel ODS-100Z column (4.6 …

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Composition Of Phenolic Compounds And Antioxidant Attributes Of Cyclea Gracillima Diels Extracts, J.-T. Lin, S.-C. Liu, L.-C. Kuo, D.-J. Yang Jul 2020

Composition Of Phenolic Compounds And Antioxidant Attributes Of Cyclea Gracillima Diels Extracts, J.-T. Lin, S.-C. Liu, L.-C. Kuo, D.-J. Yang

Journal of Food and Drug Analysis

Cyclea gracillima Diels is a Taiwanese native medicinal herb. However, there are currently few relevant reports on its biochemical activity. In this study, the antioxidant attributes of the ethanol and hot water extracts of this herb were assayed using in vitro models, including the following: 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazyl radical scavenging, Trolox equivalent antioxidant capacity, reducing power, and chelating ferrous ions. The following biochemical models were also assayed: inhibition of human low density lipoprotein oxidation, inhibition of human erythrocyte hemolysis, and scavenging oxygen radicals in human blood. The composition and content of flavonoids and phenolic acids in these extracts were also analyzed. The …

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Anti-Inflammatory Effect Of Cinnamaldehyde And Linalool From The Leaf Essential Oil Of Cinnamomum Osmophloeum Kanehira In Endotoxin-Induced Mice, S.-C. Lee, S.-Y. Wang, C.-C. Li, C.-T. Liu Jul 2020

Anti-Inflammatory Effect Of Cinnamaldehyde And Linalool From The Leaf Essential Oil Of Cinnamomum Osmophloeum Kanehira In Endotoxin-Induced Mice, S.-C. Lee, S.-Y. Wang, C.-C. Li, C.-T. Liu

Journal of Food and Drug Analysis

Cinnamomum osmophloeum Kanehira is a Taiwan native plant that belongs to genus Cinnamomum and is also known as pseudocinnamomum or indigenous cinnamon. Its leaf is traditionally used by local people in cooking and as folk therapy. We previously demonstrated the chemical composition and anti-inflammatory effect of leaf essential oil of Cinnamomum osmophloeum Kanehira of linalool chemotype in streptozotocin-induced diabetic rats and on endotoxin-injected mice. The aim of the present study is to evaluate whether cinnamaldehyde and linalool the active anti-inflammatory compounds in leaf essential oil of Cinnamomum osmophloeum Kanehira. Before the injection of endotoxin, C57BL/6 mice of the experimental groups …

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Selected Essential Oils Inhibit Key Physiological Enzymes And Possess Intracellular And Extracellular Antimelanogenic Properties In Vitro, Z. Aumeeruddy-Elalfi, N. Lall, B. Fibrich, A. Blom Van Staden, M. Hosenally, M.F. Mahomoodally Jul 2020

Selected Essential Oils Inhibit Key Physiological Enzymes And Possess Intracellular And Extracellular Antimelanogenic Properties In Vitro, Z. Aumeeruddy-Elalfi, N. Lall, B. Fibrich, A. Blom Van Staden, M. Hosenally, M.F. Mahomoodally

Journal of Food and Drug Analysis

Essential oils (EOs) extracted from six medicinal herbs and food plants [Cinnamomum zeylanicum (CZ), Psiadia arguta (PA), Psiadia terebinthina (PT), Citrus grandis (CGp), Citrus hystrix (CH), and Citrus reticulata (CR)] were studied for any inhibitory potential against key physiological enzymes involved in diabetes (α-glucosidase), skin aging (collagenase and elastase), and neurodegenerative disorders (acetylcholinesterase). Kinetic studies of the active EOs on the aforementioned enzymes were determined using Lineweaver–Burk plots. The intracellular and extracellular antimelanogenic potential of the EOs were evaluated on B16F10 mouse melanocytes. CH and CR were found to significantly inhibit (2.476 ± 0.13 μg/mL and 3.636 ± 0.10 μg/mL, …

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Characterization Of An Extracellular Β-Glucosidase From Dekkera Bruxellensis For Resveratrol Production, H.-P. Kuo, R. Wang, C.-Y. Huang, J.-T. Lai, Y.-C. Lo, S.-T. Huang Jul 2020

Characterization Of An Extracellular Β-Glucosidase From Dekkera Bruxellensis For Resveratrol Production, H.-P. Kuo, R. Wang, C.-Y. Huang, J.-T. Lai, Y.-C. Lo, S.-T. Huang

Journal of Food and Drug Analysis

Polygonum cuspidatum is a widely grown crop with a rich source of polydatin (also called piceid) for resveratrol production. Resveratrol is produced from piceid via enzymatic cleavage of the sugar moiety of piceid. In this study, Dekkera bruxellensis mutants were selected based on their high p-nitrophenyl-β-D-glucopyranoside and piceid conversion activities. The enzyme responsible for piceid conversion was a heterodimeric protein complex that was predominantly secreted to the extracellular medium and consisted of two subunits at an equal ratio with molecular masses of 30.5 kDa and 48.3 kDa. The two subunits were identified as SCW4p and glucan-β-glucosidase precursor in D. bruxellensis. …

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A Novel Poly (Glycine) Biosensor Towards The Detection Of Indigo Carmine: A Voltammetric Study, J.G.G. Manjunatha Jul 2020

A Novel Poly (Glycine) Biosensor Towards The Detection Of Indigo Carmine: A Voltammetric Study, J.G.G. Manjunatha

Journal of Food and Drug Analysis

The electrochemical behavior of indigo carmine (IC) at poly (glycine) modified carbon paste electrode (PGMCPE) was investigated by cyclic and differential pulse voltammetry. The oxidation peak of IC was observed in phosphate buffer of pH 6.5. The influence of different pH, scan rate, and concentration were analyzed. The probable reaction mechanism involved in the oxidation of IC was also proposed. Results showed that PGMCPE a remarkable electrocatalytic activity for the oxidation of IC under optimal conditions. The electrocatalytic response of the sensor was proportional to the IC concentration in the range of (2 × 10 −6 –1 × 10 −5 …

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Codissolution Of Calcium Hydrogenphosphate And Sodium Hydrogencitrate In Water. Spontaneous Supersaturation Of Calcium Citrate Increasing Calcium Bioavailability, M. Vavrusova, B.P. Danielsen, A.C. Garcia, L.H. Skibsted Jul 2020

Codissolution Of Calcium Hydrogenphosphate And Sodium Hydrogencitrate In Water. Spontaneous Supersaturation Of Calcium Citrate Increasing Calcium Bioavailability, M. Vavrusova, B.P. Danielsen, A.C. Garcia, L.H. Skibsted

Journal of Food and Drug Analysis

The sparingly soluble calcium hydrogenphosphate dihydrate, co-dissolving in water during dissolution of freely soluble sodium hydrogencitrate sesquihydrate as caused by proton transfer from hydrogencitrate to hydrogenphosphate, was found to form homogenous solutions supersaturated by a factor up to 8 in calcium citrate tetrahydrate. A critical hydrogencitrate concentration for formation of homogeneous solutions was found to depend linearly on dissolved calcium hydrogenphosphate: [HCitr 2− ] = 14[CaHPO 4 ] - 0.05 at 25 °C. The lag phase for precipitation of calcium citrate tetrahydrate, as identified from FT-IR spectra, from these spontaneously formed supersaturated solutions was several hours, and the time to …

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Hepatoprotective Effect Of The Ethanol Extract Of Polygonum Orientale On Carbon Tetrachloride-Induced Acute Liver Injury In Mice, Y.-J. Chiu, S.-C. Chou, C.-S. Chiu, C.-P. Kao, K.-C. Wu, C.-J. Chen, J.-C. Tsai, W.-H. Peng Jul 2020

Hepatoprotective Effect Of The Ethanol Extract Of Polygonum Orientale On Carbon Tetrachloride-Induced Acute Liver Injury In Mice, Y.-J. Chiu, S.-C. Chou, C.-S. Chiu, C.-P. Kao, K.-C. Wu, C.-J. Chen, J.-C. Tsai, W.-H. Peng

Journal of Food and Drug Analysis

Polygonum orientale L. (Polygonaceae) fruits have various medicinal uses, but their hepatoprotective effects have not yet been studied. This study investigated the hepatoprotective activity of the ethanolic extract of P. orientale (POE) fruits against carbon tetrachloride (CCl 4 )-induced acute liver injury (ALI). Mice were pretreated with POE (0.1, 0.5, and 1.0 g/kg) or silymarin (0.2 g/kg) for 5 consecutive days and administered a dose of 0.175% CCl 4 (ip) on the 5th day to induce ALI. Blood and liver samples were collected to measure antioxidative activity and cytokines. The bioactive components of POE were identified through high-performance liquid chromatography …

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Non-Destructive Profiling Of Volatile Organic Compounds Using Hs-Spme/Gc–Ms And Its Application For The Geographical Discrimination Of White Rice, D.K. Lim, C. Mo, D.-K. Lee, N.P. Long, J. Lim, S.W. Kwon Jul 2020

Non-Destructive Profiling Of Volatile Organic Compounds Using Hs-Spme/Gc–Ms And Its Application For The Geographical Discrimination Of White Rice, D.K. Lim, C. Mo, D.-K. Lee, N.P. Long, J. Lim, S.W. Kwon

Journal of Food and Drug Analysis

The authenticity determination of white rice is crucial to prevent deceptive origin labeling and dishonest trading. However, a non-destructive and comprehensive method for rapidly discriminating the geographical origins of white rice between countries is still lacking. In the current study, we developed a volatile organic compound based geographical discrimination method using headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME/GC–MS) to discriminate rice samples from Korea and China. A partial least squares discriminant analysis (PLS-DA) model exhibited a good classification of white rice between Korea and China (accuracy = 0.958, goodness of fit = 0.937, goodness of prediction = 0.831, …

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A Randomized, Double-Blind Clinical Study Of The Effects Of Ankascin 568 Plus On Blood Lipid Regulation, S.-F. Liu, Y.-R. Wang, Y.-C. Shen, C.-L. Chen, C.-N. Huang, T.-M. Pan, C.-K. Wang Jul 2020

A Randomized, Double-Blind Clinical Study Of The Effects Of Ankascin 568 Plus On Blood Lipid Regulation, S.-F. Liu, Y.-R. Wang, Y.-C. Shen, C.-L. Chen, C.-N. Huang, T.-M. Pan, C.-K. Wang

Journal of Food and Drug Analysis

Hyperlipidemia and inflammation play important roles in the development and progression of atherosclerosis. Atherosclerosis is regarded as an inflammatory response of blood vessels to injury at the start of atherosclerotic plaque formation, which then leads to cardiovascular events. Edible fungi of the Monascus species have been used as traditional Chinese medicines in East Asia for several centuries. The fermented products of Monascus purpureus NTU 568 possess a number of functional secondary metabolites including the anti-inflammatory pigments monascin and ankaflavin. Compounds derived from M. purpureus have been shown to have hypolipidemic effects. We aimed to evaluate the effects of M. purpureus …

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Efficacy Of Protein Rich Pearl Powder On Antioxidant Status In A Randomized Placebo-Controlled Trial, H.-F. Chiu, S.-C. Hsiao, Y.-Y. Lu, Y.-C. Han, Y.-C. Shen, K. Venkatakrishnan, C.-K. Wang Jul 2020

Efficacy Of Protein Rich Pearl Powder On Antioxidant Status In A Randomized Placebo-Controlled Trial, H.-F. Chiu, S.-C. Hsiao, Y.-Y. Lu, Y.-C. Han, Y.-C. Shen, K. Venkatakrishnan, C.-K. Wang

Journal of Food and Drug Analysis

Pearl is one of the well-known traditional Chinese medicine (TCM) prescribed for treating various skin and bone related disorders due to its abundant proteins and mineral contents. The present investigation focused on antioxidation and life span prolonging effects from different extracts of pearl powder. During in vitro studies, various oxidative indices were evaluated, along with lifespan-prolonging effect were checked using wild-type Caenorhabditis elegans. For the clinical trial, 20 healthy middle-aged subjects were recruited and separated into 2 groups as experimental and placebo group, who received 3 g of pearl powder/d (n = 10) and 3 g of placebo/d (n = …

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