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2020

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Articles 631 - 660 of 793

Full-Text Articles in Medicinal Chemistry and Pharmaceutics

Methods For Efficient Analysis Of Tocopherols, Tocotrienols And Their Metabolites In Animal Samples With Hplc-Ec, M.-J. Lee, W. Feng, L. Yang, Y.-K. Chen, E. Chi, A. Liu, C.S. Yang Jul 2020

Methods For Efficient Analysis Of Tocopherols, Tocotrienols And Their Metabolites In Animal Samples With Hplc-Ec, M.-J. Lee, W. Feng, L. Yang, Y.-K. Chen, E. Chi, A. Liu, C.S. Yang

Journal of Food and Drug Analysis

Tocopherols and tocotrienols, collectively known as vitamin E, have received a great deal of attention because of their interesting biological activities. In the present study, we reexamined and improved previous methods of sample preparation and the conditions of high-performance liquid chromatography for more accurate quantification of tocopherols, tocotrienols and their major chain-degradation metabolites. For the analysis of serum tocopherols/tocotrienols, we reconfirmed our method of mixing serum with ethanol followed by hexane extraction. For the analysis of tissue samples, we improved our methods by extracting tocopherols/tocotrienols directly from tissue homogenate with hexane. For the analysis of total amounts (conjugated and unconjugated …

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Use Of A Bayesian Approach In The Design And Evaluation Of Nce2s, C.-Y. Wang, L.-C. Chang, M.-S. Lin, C.-F. Hsiao, J.-D. Huang Jul 2020

Use Of A Bayesian Approach In The Design And Evaluation Of Nce2s, C.-Y. Wang, L.-C. Chang, M.-S. Lin, C.-F. Hsiao, J.-D. Huang

Journal of Food and Drug Analysis

Taiwan's regulatory agency defines New Chemical Entity 2 (NCE2) as a compound drug that has been approved and marketed for ten years in a top-ten pharmaceutically-advanced country but which is new in Taiwan. To apply for registration of NCE2 in Taiwan, a clinical trial may be conducted in Taiwan to evaluate the efficacy and safety. Since the NCE2 has been approved in at least one of the top-ten pharmaceutically-advanced countries, we can borrow the information from all of the observed data from other countries to synthesize the data from both Taiwan and other countries to assess the NCE2 efficacy. In …

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Stem Cell Therapy On Skin: Mechanisms, Recent Advances And Drug Reviewing Issues, G.-Y. Chu, Y.-F. Chen, H.-Y. Chen, M.-H. Chan, C.-S. Gau, S.-M. Weng Jul 2020

Stem Cell Therapy On Skin: Mechanisms, Recent Advances And Drug Reviewing Issues, G.-Y. Chu, Y.-F. Chen, H.-Y. Chen, M.-H. Chan, C.-S. Gau, S.-M. Weng

Journal of Food and Drug Analysis

Stem cell products and its clinical applications have been widely discussed in recent years, particularly when the Japanese “induced pluripotent stem cells” founder Dr. Yamanaka was awarded as Nobel Prize laureate in 2013. For decades, major progresses have been achieved in the stem cell biology field, and more and more evidence showed that skin stem cells are involved in the process of skin repair. Stem/progenitor cells of the epidermis are recognized to play the most essential role in the tissue regeneration of skin. In this review, we first illustrated basic stem cell characteristics and various stem cell subtypes resided in …

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Development And Validation Of An Lc–Ms/Ms Method For Quantification Of Nc-8 In Rat Plasma And Its Application To Pharmacokinetic Studies, B.H. Kachingwe, Y.-S. Uang, T.-J. Huang, L.-H. Wang, S.-J. Lin Jul 2020

Development And Validation Of An Lc–Ms/Ms Method For Quantification Of Nc-8 In Rat Plasma And Its Application To Pharmacokinetic Studies, B.H. Kachingwe, Y.-S. Uang, T.-J. Huang, L.-H. Wang, S.-J. Lin

Journal of Food and Drug Analysis

ent-16-Oxobeyeran-19-N-methylureido (NC-8) is a recently synthesized derivative of isosteviol that showed anti-hepatitis B virus (HBV) activity by disturbing replication and gene expression of the HBV and by inhibiting the host toll-like receptor 2/nuclear factor-κB signaling pathway. To study its pharmacokinetics as a part of the drug development process, a highly sensitive, rapid, and reliable liquid chromatography tandem mass spectrometry (LC–MS/MS) method was developed and validated for determining NC-8 in rat plasma. After protein precipitation extraction, the chromatographic separation of the analyte and internal standard (IS; diclofenac sodium) was performed on a reverse-phase Luna C18 column coupled with a Quattro Ultima …

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Studies On Prevention Of Obesity, Metabolic Syndrome, Diabetes, Cardiovascular Diseases And Cancer By Tea, C.S. Yang, H. Wang, Z.P. Sheridan Jul 2020

Studies On Prevention Of Obesity, Metabolic Syndrome, Diabetes, Cardiovascular Diseases And Cancer By Tea, C.S. Yang, H. Wang, Z.P. Sheridan

Journal of Food and Drug Analysis

Tea, a popular beverage made from leaves of the plant Camellia sinensis, has been studied extensively in recent decades for its beneficial health effects in the prevention of obesity, metabolic syndrome, diabetes, cancer, and other diseases. Whereas these beneficial effects have been convincingly demonstrated in most laboratory studies, results from human studies have not been consistent. Some studies demonstrated that weight reduction, alleviation of metabolic syndrome and risk reduction in diabetes were only observed in individuals who consume 3–4 cups of tea (600–900 mg tea catechins) or more daily. This chapter reviews some of these studies, the possible mechanisms of …

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A Novel Protein From Edible Fungi Cordyceps Militaris That Induces Apoptosis, K.-C. Bai, F. Sheu Jul 2020

A Novel Protein From Edible Fungi Cordyceps Militaris That Induces Apoptosis, K.-C. Bai, F. Sheu

Journal of Food and Drug Analysis

Cordyceps militaris is a dietary therapeutic fungus that is an important model species in Cordyceps research. In this study, we purified a novel protein from the fruit bodies of C. militaris and designated it as Cordyceps militaris protein (CMP). CMP has a molecular mass of 18.0 kDa and is not glycosylated. Interestingly, CMP inhibited cell viability in murine primary cells and other cell lines in a time- and dose-dependent manner. Using trypan blue staining and a lactate dehydrogenase release assay, we showed that CMP caused cell death in the murine hepatoma cell line BNL 1MEA.7R.1. Furthermore, the frequency of BNL …

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Corrigendum To “Investigation Of Borneols Sold In Taiwan By Chiral Gas Chromatography” (Journal Of Food And Drug Analysis (2018) 26(1) (348–352), (S1021949816301612) (10.1016/J.Jfda.2016.10.012)), T.-J. Ho, C.-C. Hung, T.-L. Shih, L.-M. Yiin, H.-P. Chen Jul 2020

Corrigendum To “Investigation Of Borneols Sold In Taiwan By Chiral Gas Chromatography” (Journal Of Food And Drug Analysis (2018) 26(1) (348–352), (S1021949816301612) (10.1016/J.Jfda.2016.10.012)), T.-J. Ho, C.-C. Hung, T.-L. Shih, L.-M. Yiin, H.-P. Chen

Journal of Food and Drug Analysis

Full Text: 1. In Graphic abstract, Figure 4 and Table 2, the labels for (+)-isoborneol and (−)-isoborneol should be switched. 2. 1. In Figure 1, the labels for d-isoborneol and l-isoborneol should be switched. 3. On Page 349, in Figure 2, the label for (+)-isoborneol should be (−)-isoborneol. 4. On Page 350, in Section 3.1, the first line of the second paragraph, “(+)-Isoborneol, synthesized as previously described …. ” should be “(−)-Isoborneol, synthesized as previously described …. ”. 5. On Page 351, in the legend for Figure 4, the order of (+)-isoborneol, (−)-isoborneol should be switched. © 2018

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Therapeutic Effects Of Lactobacillus Paracasei Subsp. Paracasei Ntu 101 Powder On Dextran Sulfate Sodium-Induced Colitis In Mice, C.-L. Chen, P.-Y. Hsu, T.-M. Pan Jul 2020

Therapeutic Effects Of Lactobacillus Paracasei Subsp. Paracasei Ntu 101 Powder On Dextran Sulfate Sodium-Induced Colitis In Mice, C.-L. Chen, P.-Y. Hsu, T.-M. Pan

Journal of Food and Drug Analysis

Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) whose exact cause is still unclear. Disruption of the intestinal microflora is considered one of the main causes of the disease. Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) is a multifunctional strain that has been shown in previous studies to possess anti-inflammatory properties and to exert a modulatory effect on intestinal bacteria associated with certain pathogenic mechanisms of IBD. In the current study, we investigated the effects of NTU 101 on dextran sulfate sodium (DSS)-induced colitis in a mouse model. Colitis was induced in male C57BL/6 mice (total …

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Down-Regulation Of Partial Substitution For Staple Food By Oat Noodles On Blood Lipid Levels: A Randomized, Double-Blind, Clinical Trial, M.-Y. Liao, Y.-C. Shen, H.-F. Chiu, S.-M. Ten, Y.-Y. Lu, Y.-C. Han, K. Venkatakrishnan, S.-F. Yang, C.-K. Wang Jul 2020

Down-Regulation Of Partial Substitution For Staple Food By Oat Noodles On Blood Lipid Levels: A Randomized, Double-Blind, Clinical Trial, M.-Y. Liao, Y.-C. Shen, H.-F. Chiu, S.-M. Ten, Y.-Y. Lu, Y.-C. Han, K. Venkatakrishnan, S.-F. Yang, C.-K. Wang

Journal of Food and Drug Analysis

This clinical trial was conducted to assess the lipid-lowering activity of oat noodles by replacing partial staple food (wheat or rice noodle) in normal and mildly hypercholesterolemic subjects. Totally 84 healthy and mild hypercholesterolemic subjects were recruited and divided into 2 groups as experimental (oat noodles) and placebo (wheat noodles) and instructed to consume 100 g of oat noodles or wheat noodles (replacing one or two meals/day) for 10 weeks and followed by 2 weeks of follow up (without noodle consumption). Various anthropometric measurements and biochemical analysis were carried out during initial (baseline), 2nd, 6th, 10th and 12th week (follow-up). …

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A Steroidal Derivative From Trigonella Foenum Graecum L. That Induces Apoptosis In Vitro And In Vivo, R.S. Thakur, B. Ahirwar Jul 2020

A Steroidal Derivative From Trigonella Foenum Graecum L. That Induces Apoptosis In Vitro And In Vivo, R.S. Thakur, B. Ahirwar

Journal of Food and Drug Analysis

Trigonella foenum graecum L. is a dietary herb used in traditional medicine system. In this study, we investigated the cytotoxicity, antitumor, antimetastatic and antiangiogenic effect of the steroidal compound, ethyl iso-allocholate isolated from T. foenum graecum L. seeds against A549 lung cancer cells in vitro and in vivo. Among all the isolated compounds, the ethyl iso-allocholate rendered the highest cytotoxicity potential. It showed least percentage cell viability in trypan blue assay and lowest nuclei count in hoechst staining. The caspase glo assay and western blot analysis showed the significant caspase 3 cleavage, indicating caspase dependent apoptosis. Consistent with the in …

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Multivariate Optimization Of Mebeverine Analysis Using Molecularly Imprinted Polymer Electrochemical Sensor Based On Silver Nanoparticles, A. Nezhadali, G.A. Bonakdar Jul 2020

Multivariate Optimization Of Mebeverine Analysis Using Molecularly Imprinted Polymer Electrochemical Sensor Based On Silver Nanoparticles, A. Nezhadali, G.A. Bonakdar

Journal of Food and Drug Analysis

Thin film of a moleculary imprinted polymer (MIP) based on electropolymerization method with sensitive and selective binding sites for mebeverine (MEB) was developed. This film was cast on pencil graphite electrode (PGE) by electrochemical polymerization in solution of pyrrole (PY) and template MEB via cyclic voltammetry scans and further electrodeposition of silver nanoparticles (AgNPs). Several parameters controlling the performance of the silver nano particles MIP pencil graphite electrode (AgNPs-MIP-PGE) including concentration of PY(mM) concentration of mebeverine (mM), number of cycles in electropolymerization, scan rate of CV process (mV. s−1), deposition time of AgNPs on to the MIP surface (s), stirring …

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Isoprenyl Phenolic Ethers From The Termite Nest-Derived Medicinal Fungus Xylaria Fimbriata, M.-C. Chen, G.-J. Wang, Y.-H. Kuo, Y.-R. Chiang, T.-Y. Cho, Y.-M. Ju, T.-H. Lee Jul 2020

Isoprenyl Phenolic Ethers From The Termite Nest-Derived Medicinal Fungus Xylaria Fimbriata, M.-C. Chen, G.-J. Wang, Y.-H. Kuo, Y.-R. Chiang, T.-Y. Cho, Y.-M. Ju, T.-H. Lee

Journal of Food and Drug Analysis

Seven new isoprenyl phenolic ethers, namely fimbriethers A‒G (1‒7), were isolated from the fermented broth of the termite nest-derived medicinal fungus Xylaria fimbriata YMJ491. Their structures were determined by spectroscopic data analysis and compared with those reported. The effects of all the isolates at a concentration of 100 μM on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells were evaluated, and all of them exhibited NO production inhibitory activity with Emax values ranging from 4.6 ± 2.0% to 49.7 ± 0.5% without significant cytotoxicity. In addition, these seven compounds did not alter phenylephrine-induced …

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Hantzsch Pre-Column Derivatization For Simultaneous Determination Of Alendronate Sodium And Its Pharmacopoeial Related Impurity: Comparative Study With Synchronous Fluorometry Using Fluorescamine, A.F. El-Yazbi, E.I. El-Kimary, R.M. Youssef Jul 2020

Hantzsch Pre-Column Derivatization For Simultaneous Determination Of Alendronate Sodium And Its Pharmacopoeial Related Impurity: Comparative Study With Synchronous Fluorometry Using Fluorescamine, A.F. El-Yazbi, E.I. El-Kimary, R.M. Youssef

Journal of Food and Drug Analysis

High performance liquid chromatographic (HPLC) method with a pre-column derivatization based on Hantzsch condensation reaction was applied for simultaneous determination of alendronate sodium (ALN) and its main related impurity, 4-Aminobutanoic acid (ABA) at its pharmacopeial limit. The separation of colored condensation products of ALN and ABA were achieved on Agilent Zobrax Eclipse SB-C18 analytical column (250 × 4.6 mm, 5 μm) using a mobile phase composed of acetonitrile–0.1 M acetate buffer, pH 5.0 (15:85, v/v). The flow rate was 1 mL min−1. The detection was carried out at 340 nm using photo-diode array detector. Peak areas were used for the …

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In Vitro Α-Amylase And Pancreatic Lipase Inhibitory Activity Of Cornus Mas L. And Cornus Alba L. Fruit Extracts, A. Świerczewska, T. Buchholz, M.F. Melzig, M.E. Czerwińska Jul 2020

In Vitro Α-Amylase And Pancreatic Lipase Inhibitory Activity Of Cornus Mas L. And Cornus Alba L. Fruit Extracts, A. Świerczewska, T. Buchholz, M.F. Melzig, M.E. Czerwińska

Journal of Food and Drug Analysis

Less-common fruits from Cornus spp. (Cornaceae), also named dogwoods, have shown antidiabetic, antibacterial and anti-allergic properties and are thus considered a source of phytochemicals that are beneficial to human health. The study aimed to compare the chemical compositions of the aqueous and ethanolic extracts of lyophilized fresh-picked and commercially available dried fruits of Cornus mas (Cm, cornelian cherry) and Cornus alba (Ca) fruits using HPLC-DAD-MS/MS method. Simultaneously, the α-amylase and pancreatic lipase (PL) inhibitory activities of the prepared extracts were compared by in vitro fluorescence assay based on the kinetic hydrolysis of starch or oleate ester of 4-methylumbelliferone (MUO), respectively. …

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Comparative Study On Amount Of Nutraceuticals In By-Products From Solvent And Cold Pressing Methods Of Rice Bran Oil Processing, D. Wongwaiwech, M. Weerawatanakorn, S. Tharatha, C.-T. Ho Jul 2020

Comparative Study On Amount Of Nutraceuticals In By-Products From Solvent And Cold Pressing Methods Of Rice Bran Oil Processing, D. Wongwaiwech, M. Weerawatanakorn, S. Tharatha, C.-T. Ho

Journal of Food and Drug Analysis

Rice bran oil (RBO) has become a popular oil globally. However, the RBO extraction process leaves various residue products, which contain bioactive substances of varying potency which could be significant sources of functional ingredients for both food production and pharmaceutical manufacture. The objective of our study was to compare the bioactive substances in various by-products derived from the two rice bran oil processing methods; solvent extraction and cold pressing. The residues from solvent extraction processing contained up to 97.37 mg/100 g of γ-aminobutyric acid in defatted rice bran, and the rice acid oil contained high levels of vitamin E (tocopherols, …

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Raman Spectroscopy Coupled With Chemometric Methods For The Discrimination Of Foreign Fats And Oils In Cream And Yogurt, Karacaglar N.N. Yazgan, T. Bulat, I.H. Boyaci, A. Topcu Jul 2020

Raman Spectroscopy Coupled With Chemometric Methods For The Discrimination Of Foreign Fats And Oils In Cream And Yogurt, Karacaglar N.N. Yazgan, T. Bulat, I.H. Boyaci, A. Topcu

Journal of Food and Drug Analysis

The adulteration of milk fat in dairy products with cheaper non-milk based fats or oils is frequently encountered in the dairy industry. In this study, Raman spectroscopy with chemometric was used for the discrimination of foreign fats and oils in milk cream and yogurt. Firstly, binary mixtures of cream and oils (corn and sunflower oil), and vegetable fat blends which are potentially or currently used by the dairy industry were prepared. All fat or oil samples and their binary mixtures were examined by using Raman spectroscopy. Then, fat content of skim milk was adjusted to 3% (w/w) by the milk …

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Prioritization Of Pesticides In Crops With A Semi-Quantitative Risk Ranking Method For Taiwan Postmarket Monitoring Program, W.-C. Chou, W.-R. Tsai, H.-H. Chang, S.-Y. Lu, K.-F. Lin, P. Lin Jul 2020

Prioritization Of Pesticides In Crops With A Semi-Quantitative Risk Ranking Method For Taiwan Postmarket Monitoring Program, W.-C. Chou, W.-R. Tsai, H.-H. Chang, S.-Y. Lu, K.-F. Lin, P. Lin

Journal of Food and Drug Analysis

A risk-based prioritization of chemical hazards in monitoring programs allows regulatory agencies to focus on the most potentially concerned items involving human health risk. In this study, a risk-based matrix, with a scoring method using multiple factors for severity and probability of exposure, was employed to identify the pesticides presented in crops that may pose the greatest risk to human health. Both the probability of exposure and the severity were assessed for 91 pesticides detected in a Taiwanese postmarketing monitoring program. Probability of exposure was evaluated based on the probability of consumption and evidence of pesticide residues in crops. Severity …

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Effect Of Sinapic Acid On Aripiprazole Pharmacokinetics In Rats: Possible Food Drug Interaction, M. Raish, A. Ahmad, M.A. Ansari, K.M. Alkharfy, A. Ahad, A. Khan, F.I. Aljenobi, N. Ali, A.M. Al-Mohizea Jul 2020

Effect Of Sinapic Acid On Aripiprazole Pharmacokinetics In Rats: Possible Food Drug Interaction, M. Raish, A. Ahmad, M.A. Ansari, K.M. Alkharfy, A. Ahad, A. Khan, F.I. Aljenobi, N. Ali, A.M. Al-Mohizea

Journal of Food and Drug Analysis

Dietary supplements and foods can interact with various drugs, leading to possible clinical concerns. This study aimed to investigate the effect of orally administered sinapic acid (SA) on the pharmacokinetics of aripiprazole (APZ) in rats and its possible modulatory effects on hepatic cytochrome P450 (CYP3A2 and CYP2D6) expression in the liver tissues. Single dose and multiple dose parallel groups of wistar rats were categorized into six groups (n = 6 each) which abstained from food for 12 h prior to the experiment, while water was allowed ad libitum. The investigation was carried out for single dose: Group I was treated …

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Catechin Rich Butanol Fraction Extracted From Acacia Catechu L. (A Thirst Quencher) Exhibits Immunostimulatory Potential, M.A. Sunil, V.S. Sunitha, A. Ashitha, S. Neethu, S.J. Midhun, E.K. Radhakrishnan, M. Jyothis Jul 2020

Catechin Rich Butanol Fraction Extracted From Acacia Catechu L. (A Thirst Quencher) Exhibits Immunostimulatory Potential, M.A. Sunil, V.S. Sunitha, A. Ashitha, S. Neethu, S.J. Midhun, E.K. Radhakrishnan, M. Jyothis

Journal of Food and Drug Analysis

Acacia catechu L., (Fabaceae) named as “catechu” is a plant, the decoction of heartwood of which is daily consumed as thirst quencher by a good percentage of the population in South India. The plant is mainly distributed in India and other Asian countries. It has been used in Indian traditional medicine for the treatment of asthma, bronchitis, colic, diarrhea, boils, skin afflictions, sores and stomatitis. The present investigation was aimed to study the immunomodulatory effects of different fractions of ethanol extract of A. catechu heartwood and HPLC analysis of the active fraction. Three fractions namely, butanol, chloroform and ethyl acetate …

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Multiclass Analysis Of Antimicrobial Drugs In Shrimp Muscle By Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry, S. Susakate, S. Poapolathep, C. Chokejaroenrat, P. Tanhan, J. Hajslova, M. Giorgi, K. Saimek, Z. Zhang, A. Poapolathep Jul 2020

Multiclass Analysis Of Antimicrobial Drugs In Shrimp Muscle By Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry, S. Susakate, S. Poapolathep, C. Chokejaroenrat, P. Tanhan, J. Hajslova, M. Giorgi, K. Saimek, Z. Zhang, A. Poapolathep

Journal of Food and Drug Analysis

A reliable, selective and rapid multiclass method has been developed for the simultaneous determination of 55 antibacterial drug residues in shrimp muscle samples by ultra high performance liquid chromatography-tandem mass spectrometry. The investigated compounds comprise of eight different classes, namely fluoroquinolones, sulfonamides and synergistic agents, tetracyclines, macrolides, lincosamides, penicillins, nitroimidazole and amphenicols. A simple liquid extraction procedure was developed consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA), followed by a defatting step with n-hexane. Chromatographic conditions were optimized, obtaining a running time 74.3% to 113.3%. For precision test, relative standard deviations (RSD, %) were lower than …

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Rgo-Ns Sers-Based Coupled Chemometric Prediction Of Acetamiprid Residue In Green Tea, M.M. Hassan, Q. Chen, F.Y.H. Kutsanedzie, H. Li, M. Zareef, Y. Xu, M. Yang, A.A. Agyekum Jul 2020

Rgo-Ns Sers-Based Coupled Chemometric Prediction Of Acetamiprid Residue In Green Tea, M.M. Hassan, Q. Chen, F.Y.H. Kutsanedzie, H. Li, M. Zareef, Y. Xu, M. Yang, A.A. Agyekum

Journal of Food and Drug Analysis

Pesticide residue in food is of grave concern in recent years. In this paper, a rapid, sensitive, SERS (Surface-enhanced Raman scattering) active reduced-graphene-oxide-gold-nano-star (rGO-NS) nano-composite nanosensor was developed for the detection of acetamiprid (AC) residue in green tea. Different concentrations of AC combined with rGO-NS nano-composite electro-statically, yielded a strong SERS signal linearly with increasing concentration of AC ranging from 1.0 × 10−4 to 1.0 × 103 μg/mL indicating the potential of rGO-NS nano-composite to detect AC in green tea. Genetic algorithm-partial least squares regression (GA-PLS) algorithm was used to develop a quantitative model for AC residue prediction. The GA-PLS …

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Development And Validation Of A New Analytical Hplc Method For Simultaneous Determination Of The Antidiabetic Drugs, Metformin And Gliclazide, A. Gedawy, H. Al-Salami, C.R. Dass Jul 2020

Development And Validation Of A New Analytical Hplc Method For Simultaneous Determination Of The Antidiabetic Drugs, Metformin And Gliclazide, A. Gedawy, H. Al-Salami, C.R. Dass

Journal of Food and Drug Analysis

An efficient and simple HPLC method has been developed and validated for the simultaneous determination of gliclazide and metformin hydrochloride in bulk and was applied on marketed metformin and gliclazide products. The mobile phase used for the chromatographic runs consisted of 20 mM ammonium formate buffer (pH 3.5) and acetonitrile (45:55, v/v) The separation was achieved on an Alltima CN (250 mm × 4.6 mm x5μ) column using isocratic mode. Drug peaks were well separated and were detected by a UV detector at 227 nm. The method was linear at the concentration range 1.25–150 μg/ml for gliclazide and 2.5–150 μg/ml …

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A Rapid Lc-Ms/Ms Method For Simultaneous Determination Of Quetiapine And Duloxetine In Rat Plasma And Its Application To Pharmacokinetic Interaction Study, X. Chen, C. Liang, L. Cui, J. Le, Z. Qian, R. Zhang, Z. Hong, Y. Chai Jul 2020

A Rapid Lc-Ms/Ms Method For Simultaneous Determination Of Quetiapine And Duloxetine In Rat Plasma And Its Application To Pharmacokinetic Interaction Study, X. Chen, C. Liang, L. Cui, J. Le, Z. Qian, R. Zhang, Z. Hong, Y. Chai

Journal of Food and Drug Analysis

Combinations of new antidepressants like duloxetine and second-generation antipsychotics like quetiapine are used in clinical treatment of major depressive disorder, as well as in forensic toxicology scenarios. The drug–drug interaction (DDI) between quetiapine and duloxetine is worthy of attention to avoid unnecessary adverse effects. However, no pharmacokinetic DDI studies of quetiapine and duloxetine have been reported. In the present study, a rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of quetiapine and duloxetine in rat plasma. A one-step protein precipitation with acetonitrile was applied for sample preparation. The analytes were eluted on an …

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Abelmoschus Esculentus Subfractions Improved Nephropathy With Regulating Dipeptidyl Peptidase-4 And Type 1 Glucagon-Like Peptide Receptor In Type 2 Diabetic Rats, C.-H. Peng, H.-C. Lin, C.-L. Lin, C.-J. Wang, C.-N. Huang Jul 2020

Abelmoschus Esculentus Subfractions Improved Nephropathy With Regulating Dipeptidyl Peptidase-4 And Type 1 Glucagon-Like Peptide Receptor In Type 2 Diabetic Rats, C.-H. Peng, H.-C. Lin, C.-L. Lin, C.-J. Wang, C.-N. Huang

Journal of Food and Drug Analysis

Abelmoschus esculentus (AE) has been used in traditional medicine to ameliorate hyperglycemia, but its mucilage increased bioassay difficulties. We have obtained a series of AE subfractions. Among them F1 and F2 regulated dipeptidyl peptidase-4 (DPP-4) and type 1 glucagon-like peptide receptor (GLP-1R), the treatment targets for type 2 diabetes. F1, F2 and fraction residues (FR) showed advantage on different aspects, which attenuates insulin resistance and metabolic disorder in vivo, and prevents renal-tubular change in vitro. In the present study, using type 2 diabetes model induced by high fat diet (HFD) and streptozotocin (STZ), we aim to investigate whether AE prevent …

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Encapsulation Of Babchi Essential Oil Into Microsponges: Physicochemical Properties, Cytotoxic Evaluation And Anti-Microbial Activity, G. Wadhwa, S. Kumar, V. Mittal, R. Rao Jul 2020

Encapsulation Of Babchi Essential Oil Into Microsponges: Physicochemical Properties, Cytotoxic Evaluation And Anti-Microbial Activity, G. Wadhwa, S. Kumar, V. Mittal, R. Rao

Journal of Food and Drug Analysis

Babchi essential oil (BEO) is a valuable essential oil reported to possess a variety of biological activities such as antitumor, anti inflammatory, immunomodulatory, antioxidant, antifungal and antibacterial properties. Due to its anti-microbial properties, this oil possesses an immense potential for the treatment of dermatological disorders. Further, it has minimal tendency to develop resistance, a common issue with most of the antibiotics. However, its highly viscous nature and poor stability in the presence of light, air and high temperature, limits its practical applications. To surmount these issues, this research aims to encapsulate BEO in ethyl cellulose (EC) microsponges for enhanced stability, …

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Rubiscolin-6 Activates Opioid Receptors To Enhance Glucose Uptake In Skeletal Muscle, T.S. Kairupan, K.-C. Cheng, A. Asakawa, H. Amitani, T. Yagi, K. Ataka, N.T. Rokot, N.H. Kapantow, I. Kato, A. Inui Jul 2020

Rubiscolin-6 Activates Opioid Receptors To Enhance Glucose Uptake In Skeletal Muscle, T.S. Kairupan, K.-C. Cheng, A. Asakawa, H. Amitani, T. Yagi, K. Ataka, N.T. Rokot, N.H. Kapantow, I. Kato, A. Inui

Journal of Food and Drug Analysis

Rubiscolin-6 is an opioid peptide derived from plant ribulose bisphosphate carboxylase/oxygenase (Rubisco). It has been demonstrated that opioid receptors could control glucose homeostasis in skeletal muscle independent of insulin action. Therefore, Rubiscolin-6 may be involved in the control of glucose metabolism. In the present study, we investigated the effect of rubiscolin-6 on glucose uptake in skeletal muscle. Rubiscolin-6-induced glucose uptake was measured using the fluorescent indicator 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG) in L6 and C2C12 cell lines. The protein expressions of glucose transporter 4 (GLUT4) and AMP-activated protein kinase (AMPK) in L6 cells were observed by Western blotting. The in vivo effects …

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The Rapid And Sensitive Detection Of Edible Bird's Nest (Aerodramus Fuciphagus) In Processed Food By A Loop-Mediated Isothermal Amplification (Lamp) Assay, M.-S. Lee, J.-Y. Huang, Y.-Y. Lien, S.-C. Sheu Jul 2020

The Rapid And Sensitive Detection Of Edible Bird's Nest (Aerodramus Fuciphagus) In Processed Food By A Loop-Mediated Isothermal Amplification (Lamp) Assay, M.-S. Lee, J.-Y. Huang, Y.-Y. Lien, S.-C. Sheu

Journal of Food and Drug Analysis

Edible bird's nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers’ interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of EBN using specifically designed LAMP primers. The results demonstrated that the identification of EBN by LAMP assay was specific and rapid (within 1 h). It had no cross-reaction with EBN adulterants, including white fungus, egg white and pig skin, in different ratios. The relative detection limit was 0.01% EBN in the adulterants. …

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Ubiquitin-Protein Ligase E3a (Ube3a) As A New Biomarker Of Cardiac Hypertrophy In Cell Models, K.-C. Cheng, Y. Li, W.-T. Chang, Z.-C. Chen, J.-T. Cheng, C.-C. Tsai Jul 2020

Ubiquitin-Protein Ligase E3a (Ube3a) As A New Biomarker Of Cardiac Hypertrophy In Cell Models, K.-C. Cheng, Y. Li, W.-T. Chang, Z.-C. Chen, J.-T. Cheng, C.-C. Tsai

Journal of Food and Drug Analysis

Cardiac hypertrophy is widely diagnosed in clinical cardiac disorders. The pathophysiology of hypertrophy is complex and multifactorial, a series of molecular and cellular changes are participated, such as activation of different signaling pathways, a switch of fetal gene program in the myocardium, and apoptosis. Some biomarkers have been applied to assess cardiac hypertrophy including atrial natriuretic peptides (ANP), brain/B-type natriuretic peptides (BNP), and α- or β- Myosin Heavy Chain (MHC) in addition to others. Recently, ubiquitin-protein ligase E3A (UBE3A) has been observed to increase in cardiac hypertrophy. Therefore, UBE3A as a new biomarker seems valuable in the clinic. The cardiac …

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Antidepressant And Anxiolytic Effects Of The Proprietary Chinese Medicine Shexiang Baoxin Pill In Mice With Chronic Unpredictable Mild Stress, X.-D. Zhou, D.-D. Shi, Z.-J. Zhang Jul 2020

Antidepressant And Anxiolytic Effects Of The Proprietary Chinese Medicine Shexiang Baoxin Pill In Mice With Chronic Unpredictable Mild Stress, X.-D. Zhou, D.-D. Shi, Z.-J. Zhang

Journal of Food and Drug Analysis

Depression and anxiety often co-occur with cardiac diseases. The Shexiang Baoxin pill (SBP) is a proprietary Chinese medicine initially used to treat cardiac conditions. This study explored whether SBP has antidepressant and anxiolytic effects in addition to hormonal and psychotropic mechanisms. Mice underwent 6 weeks of chronic unpredictable mild stress (CUMS) to induce depression- and anxiety-like behavior. During the 6-week experiment, mice received SBP at intragastric doses of 20.25 mg/kg or 40.5 mg/kg daily. Animals were then tested for depression in sucrose preference, forced-swimming, and tail suspension paradigms, and for anxiety in open field and elevated plus maze tests. Both …

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Novel Technologies In Detection, Treatment And Prevention Of Substance Use Disorders, H. Moulahoum, F. Zihnioglu, S. Timur, H. Coskunol Jul 2020

Novel Technologies In Detection, Treatment And Prevention Of Substance Use Disorders, H. Moulahoum, F. Zihnioglu, S. Timur, H. Coskunol

Journal of Food and Drug Analysis

Substance use disorders are a widely recognized problem, which affects various levels of communities and influenced the world socioeconomically. Its source is deeply embedded in the global population. In order to fight against such an adversary, governments have spared no efforts in implementing substance abuse treatment centers and funding research to develop treatments and prevention procedures. In this review, we will discuss the use of immunological-based treatments and detection kit technologies. We will be detailing the steps followed to produce performant antibodies (antigens, carriers, and adjuvants) focusing on cocaine and methamphetamine as examples. Furthermore, part of this review is dedicated …

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