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Articles 961 - 990 of 1074

Full-Text Articles in Medicinal Chemistry and Pharmaceutics

Utilizing Proteomic Approach To Identify Nuclear Translocation Related Serine Kinase Phosphorylation Site Of Gnmt As Downstream Effector For Benzo[A]Pyrene, M.-H. Yang, C.-C. Liao, J.-H. Hung, X.-T. Lai, C.-H. Yen, Y.-M.A. Chen Jul 2020

Utilizing Proteomic Approach To Identify Nuclear Translocation Related Serine Kinase Phosphorylation Site Of Gnmt As Downstream Effector For Benzo[A]Pyrene, M.-H. Yang, C.-C. Liao, J.-H. Hung, X.-T. Lai, C.-H. Yen, Y.-M.A. Chen

Journal of Food and Drug Analysis

Glycine N-methyltransferase (GNMT) protein is highly expressed in certain tissues, such as liver, pancreas, and prostate. GNMT serves multiple roles which include a methyl group transfer enzyme and a liver tumor suppressor. Benzo(a)pyrene (BaP), a family member of polycyclic aromatic hydrocarbon (PAH), is a known environmental carcinogen found in coal tar, tobacco smoke, barbecued food and incomplete combustion of auto fuel. BaP recruits cytochrome P450 to transform itself into benzo(a)pyrene-7,8-diol-9,10-epoxide (B(a)PDE), which covalently interacts with DNA causing tumorigenesis. BaP can be detoxified through GNMT and induces GNMT translocation into the cellular nucleus. GNMT translocation is accompanied by phosphorylation, but the …

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Detection Of Bpde-Dna Adducts In Human Umbilical Cord Blood By Lc-Ms/Ms Analysis, L. Guo, X. Jiang, H.-Y. Tian, S.-J. Yao, B.-Y. Li, R.-J. Zhang, S.-S. Zhang, X. Sun Jul 2020

Detection Of Bpde-Dna Adducts In Human Umbilical Cord Blood By Lc-Ms/Ms Analysis, L. Guo, X. Jiang, H.-Y. Tian, S.-J. Yao, B.-Y. Li, R.-J. Zhang, S.-S. Zhang, X. Sun

Journal of Food and Drug Analysis

Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to …

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A Highly Sensitive Ultra-High Performance Liquid Chromatography/Tandem Mass Spectrometry Method With In-Source Fragmentation For Rapid Quantification Of Raspberry Ketone, B. Yuan, D. Zhao, R. Du, D. Kshatriya, N.T. Bello, J.E. Simon, Q. Wu Jul 2020

A Highly Sensitive Ultra-High Performance Liquid Chromatography/Tandem Mass Spectrometry Method With In-Source Fragmentation For Rapid Quantification Of Raspberry Ketone, B. Yuan, D. Zhao, R. Du, D. Kshatriya, N.T. Bello, J.E. Simon, Q. Wu

Journal of Food and Drug Analysis

Raspberry ketone (RK) is the characteristic aromatic compound in raspberry (Rubus idaeus L.) with wide applications as food additive and anti-obesity agent. However, quantification of RK has presented difficulties in MS detection and reliable LC-MS method for RK analysis in literature is in limit to date. In order to facilitate quality control of raspberry derived products and RK metabolomics study, this study aimed to develop a validated and sensitive UHPLC-MS/MS method. Strong in-source fragmentation was noted and the fragmental ion of 107 m/z produced was selected as the precursor ion for MRM detection, and as such the electrospray ionization performance …

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Nelumbo Nucifera Leaves Extract Attenuate The Pathological Progression Of Diabetic Nephropathy In High-Fat Diet-Fed And Streptozotocin-Induced Diabetic Rats, H.-W. Chen, M.-Y. Yang, T.-W. Hung, Y.-C. Chang, C.-J. Wang Jul 2020

Nelumbo Nucifera Leaves Extract Attenuate The Pathological Progression Of Diabetic Nephropathy In High-Fat Diet-Fed And Streptozotocin-Induced Diabetic Rats, H.-W. Chen, M.-Y. Yang, T.-W. Hung, Y.-C. Chang, C.-J. Wang

Journal of Food and Drug Analysis

Diabetic nephropathy is not only a common and severe microvascular complication of diabetes mellitus but also the leading cause of renal failure. Lotus (Nelumbo nucifera) possesses antioxidative and anticancer properties. The present study aimed to investigate the antidiabetic and renoprotective effects of N. nucifera leaf extract (NLE) in a rat model of type 2 diabetic mellitus. Male Sprague–Dawley rats with type 2 diabetes induced by a high-fat diet (HFD)/streptozotocin (STZ) were treated with NLE at dosages of 0.5% and 1% (w/w) daily for 6 weeks. At the end of the experimental period, body weight, serum glucose levels, insulin levels, and …

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Risk Of Adverse Cardiovascular Events With Use Of Inhaled Long-Acting Bronchodilators In Management Of Chronic Obstructive Pulmonary Disease, M.-T. Wang, J.-H. Lai, C.-L. Tsai, J.-T. Liou Jul 2020

Risk Of Adverse Cardiovascular Events With Use Of Inhaled Long-Acting Bronchodilators In Management Of Chronic Obstructive Pulmonary Disease, M.-T. Wang, J.-H. Lai, C.-L. Tsai, J.-T. Liou

Journal of Food and Drug Analysis

Inhaled long-acting bronchodilators, including long-acting β2 agonists (LABAs) and long-acting muscarinic antagonists (LAMAs) are the mainstay therapy in the treatment of chronic obstructive pulmonary disease (COPD), a disease that poses a heavy burden on morbidity and mortality worldwide. Use of LABAs and LAMAs in patients with COPD, however, has been concerned about an increased risk of adverse cardiovascular events, despite inconsistent findings reported from randomized controlled trials (RCTs) and observational studies. In this review, we detailed the relevant evidence generated from RCTs and observational studies with respect to the risk of cardiovascular disease with use of LABAs and LAMAs in …

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A Nutraceutical Extract From Inula Viscosa Leaves: Uhplc-Hr-Ms/Ms Based Polyphenol Profile, And Antioxidant And Cytotoxic Activities, N. Brahmi-Chendouh, S. Piccolella, G. Crescente, F. Pacifico, L. Boulekbache, S. Hamri-Zeghichi, S. Akkal, K. Madani, S. Pacifico Jul 2020

A Nutraceutical Extract From Inula Viscosa Leaves: Uhplc-Hr-Ms/Ms Based Polyphenol Profile, And Antioxidant And Cytotoxic Activities, N. Brahmi-Chendouh, S. Piccolella, G. Crescente, F. Pacifico, L. Boulekbache, S. Hamri-Zeghichi, S. Akkal, K. Madani, S. Pacifico

Journal of Food and Drug Analysis

Nowadays, advanced extraction techniques and highly sensitive metabolic profiling methods are effectively employed to get new information on plant chemical constituents. Among them wild medicinal plants or their parts, with large and ancient use in folk medicine, are investigated for their potential functional use and cultivation. In this context, Inula viscosa leaves engaged our attention. A simple experimental design, based on Soxhlet extraction and chromatographic fractionation, allowed us to obtain the investigated polyphenol fraction (IvE). UHPLC-HRMS analyses revealed shikimoyl depsides of caffeic acid and unusual dihydrobenzofuran lignans as main secondary metabolites. These compounds, together with cinchonain-type phenols, and hydroxycinnamoyl flavonol …

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Direct Injection High Performance Liquid Chromatography Coupled To Data Independent Acquisition Mass Spectrometry For The Screening Of Antibiotics In Honey, Eyken A. Von, D. Furlong, S. Arooni, F. Butterworth, J.-F. Roy, J. Zweigenbaum, S. Bayen Jul 2020

Direct Injection High Performance Liquid Chromatography Coupled To Data Independent Acquisition Mass Spectrometry For The Screening Of Antibiotics In Honey, Eyken A. Von, D. Furlong, S. Arooni, F. Butterworth, J.-F. Roy, J. Zweigenbaum, S. Bayen

Journal of Food and Drug Analysis

The targeted analysis of veterinary drug residues in honey traditionally involves a series of extraction and purification steps prior to quantification with high performance liquid chromatography coupled to high resolution or tandem mass spectrometry. These steps, designed to separate the target analytes from interferences, are generally time-consuming and costly. In addition, traditional cleanup steps are likely to eliminate other compounds whose analysis could prove decisive in current or future assessment of the honey sample. Alternatively, direct injection without complex sample preparation steps has been introduced for the fast analysis of trace compounds in environmental and food matrices. The aim of …

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Revisit Gut Microbiota And Its Impact On Human Health And Disease, R.-X. Ding, W.-R. Goh, R.-N. Wu, X.-Q. Yue, X. Luo, W.W.T. Khine, J.-R. Wu, Y.-K. Lee Jul 2020

Revisit Gut Microbiota And Its Impact On Human Health And Disease, R.-X. Ding, W.-R. Goh, R.-N. Wu, X.-Q. Yue, X. Luo, W.W.T. Khine, J.-R. Wu, Y.-K. Lee

Journal of Food and Drug Analysis

Trillions of microbes have evolved with and continue to live on human beings. With the rapid advances in tools and technology in recent years, new knowledge and insight in cross-talk between the microbes and their hosts have gained. It is the aim of this work to critically review and summarize recent literature reports on the role of microbiota and mechanisms involved in the progress and development of major human diseases, which include obesity, hypertension, cardiovascular disease, diabetes, cancer, Inflammatory Bowel Disease (IBD), gout, depression and arthritis, as well as infant health and longevity. © 2019

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Next Generation Probiotics In Disease Amelioration, C.-J. Chang, T.-L. Lin, Y.-L. Tsai, T.-R. Wu, W.-F. Lai, C.-C. Lu, H.-C. Lai Jul 2020

Next Generation Probiotics In Disease Amelioration, C.-J. Chang, T.-L. Lin, Y.-L. Tsai, T.-R. Wu, W.-F. Lai, C.-C. Lu, H.-C. Lai

Journal of Food and Drug Analysis

Studies on the role of gut commensal bacteria in health development have rapidly attracted much more attention beyond the classical pathogens over the last decade. Many important reports have highlighted the changes in the gut microbiota (dysbiosis) are closely related to development of intra- and extra-intestinal, chronic inflammation related diseases such as colitis, obesity/metabolic syndromes, diabetes mellitus, liver diseases, cardiovascular diseases and also cancer and neurodegenerative diseases. To circumvent these difficulties, the strategy of modulating the structure of the gut microbiota has been under intensive study and shed more light on amelioration of these inflammation related diseases. While traditional probiotics …

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Psychobiotics In Mental Health, Neurodegenerative And Neurodevelopmental Disorders, L.-H. Cheng, Y.-W. Liu, C.-C. Wu, S. Wang, Y.-C. Tsai Jul 2020

Psychobiotics In Mental Health, Neurodegenerative And Neurodevelopmental Disorders, L.-H. Cheng, Y.-W. Liu, C.-C. Wu, S. Wang, Y.-C. Tsai

Journal of Food and Drug Analysis

Psychobiotics are a group of probiotics that affect the central nervous system (CNS) related functions and behaviors mediated by the gut-brain-axis (GBA) via immune, humoral, neural, and metabolic pathways to improve not only the gastrointestinal (GI) function but also the antidepressant and anxiolytic capacity. As a novel class of probiotics, the application of psychobiotics has led researchers to focus on a new area in neuroscience. In the past five years, some psychobiotics strains were reported to inhibit inflammation and decreased cortisol levels, resulting in an amelioration of the symptoms of anxiety and depression. Psychobiotics are efficacious in improving neurodegenerative and …

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An Ultra-Rapid Drug Screening Method For Acetaminophen In Blood Serum Based On Probe Electrospray Ionization-Tandem Mass Spectrometry, K. Usui, H. Kobayashi, Y. Fujita, E. Kubota, T. Hanazawa, T. Yoshizawa, Y. Kamijo, M. Funayama Jul 2020

An Ultra-Rapid Drug Screening Method For Acetaminophen In Blood Serum Based On Probe Electrospray Ionization-Tandem Mass Spectrometry, K. Usui, H. Kobayashi, Y. Fujita, E. Kubota, T. Hanazawa, T. Yoshizawa, Y. Kamijo, M. Funayama

Journal of Food and Drug Analysis

Poisoning incidents caused by drugs, accidental ingestion of poisons, attempted suicide, homicide, and exposure to toxic compounds occur frequently every year across the globe. This raises the need to rapidly identify toxic agents in poisoned patients in a clinical emergency setting. In addition, determining drug/poison concentration is undoubtedly necessary to arrive at a toxicological treatment plan. The purpose of this study was to develop an ultra-rapid drug screening method for the clinical treatment of poisoning. Probe electrospray ionization (PESI), one of the ambient ionization techniques, is able to detect compounds from various biological materials almost directly. We applied the PESI …

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Simultaneous Quantification Of Six Indicator Compounds In Wen-Qing-Yin By High-Performance Liquid Chromatography-Diode Array Detection, C.-C. Yeh, S.-S. Huang, P.-Y. Liu, B.-C. Wang, C.-F. Tsai, D.-Y. Wang, H.-F. Cheng Jul 2020

Simultaneous Quantification Of Six Indicator Compounds In Wen-Qing-Yin By High-Performance Liquid Chromatography-Diode Array Detection, C.-C. Yeh, S.-S. Huang, P.-Y. Liu, B.-C. Wang, C.-F. Tsai, D.-Y. Wang, H.-F. Cheng

Journal of Food and Drug Analysis

A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY). Separate optimization was performed using a Cosmosil C18 column gradient method with 0.1% formic acid in both mobile phases of aqueous and acetonitrile (ACN), at a flow rate, detection wavelength, and sample volume of 1.8 mL/min, 268 nm, and 10 μL, respectively. The linear regression of six active compounds berberine (BER), baicalin (BAI), ferulic acid (FER), geniposide (GEN), hydorxymethoxylfurfural (HMF), and paeoniflorin (PAE) was produced at the concentration …

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Potential Prebiotics And Their Transmission Mechanisms: Recent Approaches, I. Khangwal, P. Shukla Jul 2020

Potential Prebiotics And Their Transmission Mechanisms: Recent Approaches, I. Khangwal, P. Shukla

Journal of Food and Drug Analysis

Prebiotics are non-digestible carbohydrates which can be used as prime source of energy for gut microflora. These can be naturally occurring in fruit and vegetables or can be made synthetically by enzymatic digestions. New versatile sources of prebiotics had been found nowadays for economic commercialization. This review will decipher on highlighting the importance of prebiotics in immunomodulation and nutrient absorption abilities of gut, as it is important for the anti-effective capacity of the organism especially in the neonatal period. Moreover, new prebiotics transmission strategies with higher penetrating capacity such as microencapsulation and immobilization have been discussed. In addition to this, …

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Multi-Residue Analysis Using Liquid Chromatography Tandem Mass Spectrometry For Detection Of 20 Coccidiostats In Poultry, Livestock, And Aquatic Tissues, S.-H. Chang, Y.-H. Lai, C.-N. Huang, G.-J. Peng, C.-D. Liao, Y.-M. Kao, S.-H. Tseng, D.-Y. Wang Jul 2020

Multi-Residue Analysis Using Liquid Chromatography Tandem Mass Spectrometry For Detection Of 20 Coccidiostats In Poultry, Livestock, And Aquatic Tissues, S.-H. Chang, Y.-H. Lai, C.-N. Huang, G.-J. Peng, C.-D. Liao, Y.-M. Kao, S.-H. Tseng, D.-Y. Wang

Journal of Food and Drug Analysis

In this study, we developed a novel analysis method based on liquid chromatography/tandem mass spectrometry (LC–MS/MS) to allow the simultaneous identification of 20 coccidiostats in eight matrix categories, including the muscles of chicken, swine, cow, and fish as well as chicken eggs, bovine milk, and porcine viscera. In the pretreatment procedure, acetonitrile/methanol (95:5, v/v) containing 1% formic acid, 5 g of sodium acetate, and 6.0 g of anhydrous magnesium sulfate was used for extraction, followed by a clean-up procedure using n-hexane saturated with ACN to facilitate the elimination of analytes from high lipid samples. Chromatographic separations were achieved using a …

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Determination Of Phenylenediamines In Hair Colors Derivatizated With 5-(4, 6-Dichlorotriazinyl)Aminofluorescein Via Micellar Electrokinetic Chromatography, H.-Y. Ko, Y.-H. Lin, C.-J. Shih, Y.-L. Chen Jul 2020

Determination Of Phenylenediamines In Hair Colors Derivatizated With 5-(4, 6-Dichlorotriazinyl)Aminofluorescein Via Micellar Electrokinetic Chromatography, H.-Y. Ko, Y.-H. Lin, C.-J. Shih, Y.-L. Chen

Journal of Food and Drug Analysis

Phenylenediamines (PDs), which are reported to cause allergic dermatitis and possess genotoxicity and carcinogenicity, are the ingredients used in permanent hair dyes. The fluorescent derivatization strategy coupled with micellar electrokinetic chromatography (MEKC) were established to analyze four PDs, including o-phenylenediamine (OPD), m-phenylenediamine (MPD), p-phenylenediamine (PPD) and toluene-2,5-diamine (PTD). Additionally, 5-(4, 6-dichlorotriazinyl) aminofluorescein (DTAF) was used as a fluorescent reagent derived at amino groups of PDs and underwent nucleophilic substitution reaction to improve the detection sensitivity. The derivatization condition reacted at 90 °C for 10 min in alkaline conditions. The optimized separation conditions were 20 mM borate (pH 8.0) containing 10 …

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Proanthocyanidins From The Stem Bark Of Rhus Tripartita Ameliorate Methylgloxal-Induced Endothelial Cell Apoptosis, A.S. Alqahtani, W.M. Abdel-Mageed, A.A. Shahat, M.K. Parvez, M.S. Al-Dosari, A. Malik, M.S. Abdel-Kader, M.S. Alsaid Jul 2020

Proanthocyanidins From The Stem Bark Of Rhus Tripartita Ameliorate Methylgloxal-Induced Endothelial Cell Apoptosis, A.S. Alqahtani, W.M. Abdel-Mageed, A.A. Shahat, M.K. Parvez, M.S. Al-Dosari, A. Malik, M.S. Abdel-Kader, M.S. Alsaid

Journal of Food and Drug Analysis

In traditional Arabian medicine, the Rhus tripartita plant (family Anacardiaceae) has been used to treat inflammatory conditions. Although Rhus extracts have been reported for their cardioprotective effects, information regarding their active principle compounds remains insufficient. The present investigation was aimed at determining the antioxidant chemical constituents of the methanolic extract of R. tripartita stem bark and evaluating their ability to ameliorate methylglyoxal-induced endothelial cell apoptosis. Ten flavonoid compounds (1–10) were isolated and identified using DPPH radical scavenging bioassay-guided chromatographic separation. A new proanthocyanidin (rhuspartin) (1) was isolated and identified as 3,5,13,14-flavantetrol-(4β→8)-catechin, using extensive spectroscopic data and high resolution-mass spectrometry. Among …

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Determination Of Polycyclic Aromatic Hydrocarbons (Pahs) In Cosmetic Products By Gas Chromatography-Tandem Mass Spectrometry, S.-W. Wang, K.-H. Hsu, S.-C. Huang, S.-H. Tseng, D.-Y. Wang, H.-F. Cheng Jul 2020

Determination Of Polycyclic Aromatic Hydrocarbons (Pahs) In Cosmetic Products By Gas Chromatography-Tandem Mass Spectrometry, S.-W. Wang, K.-H. Hsu, S.-C. Huang, S.-H. Tseng, D.-Y. Wang, H.-F. Cheng

Journal of Food and Drug Analysis

A gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) method is developed to determine 18 representative polycyclic aromatic hydrocarbons (PAHs) in cosmetics, including Benzo[a]pyrene (BaP) and others. The method offers high sensitivity and selectivity under selected reaction monitoring (SRM) mode to satisfy the requirements of both quantitation and qualitation. The extraction solvent system used in this study is acetone/hexane 1:1 (v/v) and other purification procedure is unnecessary. The linearities of 18 PAHs are validated in different concentration in the range of 0.25–20 ng/mL individually with coefficient correlation (r) higher than 0.996. The recoveries for spiking 3 different concentrations are from …

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Comparative Quality Of The Forms Of Decoction Pieces Evaluated By Multidimensional Chemical Analysis And Chemometrics: Poria Cocos, A Pilot Study, L.-X. Zhu, J. Xu, Y. Wu, L.-F. Su, Lam K.Y. Ching, E.R. Qi, X.-P. Dong, H.-B. Chen, Y.-D. Liu, Z.-Z. Zhao Jul 2020

Comparative Quality Of The Forms Of Decoction Pieces Evaluated By Multidimensional Chemical Analysis And Chemometrics: Poria Cocos, A Pilot Study, L.-X. Zhu, J. Xu, Y. Wu, L.-F. Su, Lam K.Y. Ching, E.R. Qi, X.-P. Dong, H.-B. Chen, Y.-D. Liu, Z.-Z. Zhao

Journal of Food and Drug Analysis

Many Chinese medicinal materials (CMMs) are parts of plants or fungi that have been processed into different physical forms, termed decoction pieces, that are typically boiled in water for consumption. One CMM may have several decoction pieces forms, e.g., slices, small cubes (dice), or grains. The specifications that have different morphological parameters (shape, size and thickness) for these various decoction pieces have been developed over, in some cases, centuries of practice. Nevertheless, whether and how the form of decoction pieces affects the extraction (decoction) dynamics, and quality stability during storage has not been studied. Here, we investigated Poria cocos (PC) …

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Design, Synthesis And Evaluation Of Dna Nanocubes As A Core Material Protected By The Alginate Coating For Oral Administration Of Anti-Diabetic Drug, M.M.F.A. Baig, M. Abbas, M. Naveed, S.A. Kassim, G.J. Khan, M. Sohail, S. Ullah, M. Hasnat, K. Shah, M.T. Ansari Jul 2020

Design, Synthesis And Evaluation Of Dna Nanocubes As A Core Material Protected By The Alginate Coating For Oral Administration Of Anti-Diabetic Drug, M.M.F.A. Baig, M. Abbas, M. Naveed, S.A. Kassim, G.J. Khan, M. Sohail, S. Ullah, M. Hasnat, K. Shah, M.T. Ansari

Journal of Food and Drug Analysis

Poor control towards glycemic levels among diabetic patients may lead to severe micro/macro-vascular and neuropathic complexities. Proper functioning of alpha-beta cells of pancreases is required to attain long term glycemic control among type 2 diabetics. The recent developments to manage diabetes are focused on controlling the insulin-glucagon secretions from the pancreases. DPP-4 inhibitors class of drugs after elevating GLP-1/GIP (incretins) levels in the blood, not only raise the insulin levels but also suppress the glucagon level. Vildagliptin (VI) is a potent DPP-4 inhibitor with least adverse events compared to other DPP-4 inhibitors. We encapsulated VI into 3D nanocube that gets …

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A Sensitive Hplc-Fld Method Combined With Multivariate Analysis For The Determination Of Amino Acids In L-Citrulline Rich Vegetables, K.A. Corleto, J. Singh, G.K. Jayaprakasha, B.S. Patil Jul 2020

A Sensitive Hplc-Fld Method Combined With Multivariate Analysis For The Determination Of Amino Acids In L-Citrulline Rich Vegetables, K.A. Corleto, J. Singh, G.K. Jayaprakasha, B.S. Patil

Journal of Food and Drug Analysis

The proposed analytical method reports the separation and quantification of 21 amino acids including l-citrulline from fresh vegetables and commercial juices using a C8 column. Optimal separation conditions for amino acids analysis were obtained with 20 mM sodium acetate (solvent A) and water with organic modifier acetonitrile and methanol (solvent B; 18/50/32 V/V). The ideal pH and column temperature were found to be 5.40 and 35 °C, respectively. The LOD and LOQ values were obtained in the range of 0.02–0.19 ng/mL and 0.04–0.39 ng/mL for all amino acids respectively. Relative standard deviations (RSD) of intraday and interday analysis were found …

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The Biosimilar Pathway In The Usa: An Analysis Of The Innovator Company And Biosimilar Company Perspectives And Beyond, L.-C. Chang Jul 2020

The Biosimilar Pathway In The Usa: An Analysis Of The Innovator Company And Biosimilar Company Perspectives And Beyond, L.-C. Chang

Journal of Food and Drug Analysis

In order to improve access to costly biological treatments, a biosimilar pathway in the United States of America (USA) was enacted under the Biologics Price Competition and Innovation Act (BPCI Act) of 2009. The aim of the present study was to investigate how the health policy, the establishment of the biosimilar pathway, influenced related companies by studying their respective perspectives and strategies revealed in literatures and publicly available resources. Perspectives of companies reveal the points of concern for the biosimilar pathway, such as data requirements, patents, interchangeability, naming, and exclusivity. Innovator companies may utilize expedited programs for serious conditions, enhance …

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Effects Of Heat Treatment Of Mandarin Peel On Flavonoid Profiles And Lipid Accumulation In 3t3-L1 Adipocytes, J. Sung, J.H. Suh, Y. Wang Jul 2020

Effects Of Heat Treatment Of Mandarin Peel On Flavonoid Profiles And Lipid Accumulation In 3t3-L1 Adipocytes, J. Sung, J.H. Suh, Y. Wang

Journal of Food and Drug Analysis

Citrus peel, a primary byproduct of citrus fruits, contains a variety of flavonoids. Heat treatment is a favorable food processing for solid peel to release bioactive compounds from tissues and intensify nutritional effects. In this study, we explored alterations of flavonoids by thermal treatment of mandarin peel and their effects on lipid accumulation and intracellular levels during differentiation of 3T3-L1 cells to adipocytes. The heat-treated sample showed stronger inhibition on the formation of lipid droplets than the non-treated sample, along with enhanced intracellular levels of flavonoids. Overall flavonoids, especially flavonoid aglycones showing better efficacy, were found to increase in the …

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Systematically Characterize The Substance Basis Of Jinzhen Oral Liquid And Their Pharmacological Mechanism Using Uplc-Q-Tof/Ms Combined With Network Pharmacology Analysis, J.-Y. Guo, D.-M. Wang, M.-J. Wang, J. Zhou, Y.-N. Pan, Z.-Z. Wang, W. Xiao, X.-Q. Liu Jul 2020

Systematically Characterize The Substance Basis Of Jinzhen Oral Liquid And Their Pharmacological Mechanism Using Uplc-Q-Tof/Ms Combined With Network Pharmacology Analysis, J.-Y. Guo, D.-M. Wang, M.-J. Wang, J. Zhou, Y.-N. Pan, Z.-Z. Wang, W. Xiao, X.-Q. Liu

Journal of Food and Drug Analysis

Jinzhen oral liquid (JZ) is a classical traditional Chinese medicine formula used for the treatment of children lung disease. However, the effective substance of JZ is still unclear. In this study, we used lung injury rat model to study the protective effect of JZ, through UPLC-Q-TOF/MS detection coupled with metabolic research and network pharmacology analysis. Fortunately, 31 absorbed prototype constituents and 41 metabolites were identified or tentatively characterized based on UPLC-Q-TOF/MS analysis, and the possible metabolic pathways were hydroxylation, sulfation and glucuronidation. We optimized the data screening in the early stage of network pharmacology by collecting targets based on adsorbed …

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Estimation Of Nizatidine Gastric Nitrosatability And Product Toxicity Via An Integrated Approach Combining Hilic, In Silico Toxicology, And Molecular Docking, R. El-Shaheny, M. Radwan, K. Yamada, M. El-Maghrabey Jul 2020

Estimation Of Nizatidine Gastric Nitrosatability And Product Toxicity Via An Integrated Approach Combining Hilic, In Silico Toxicology, And Molecular Docking, R. El-Shaheny, M. Radwan, K. Yamada, M. El-Maghrabey

Journal of Food and Drug Analysis

The liability of the H2-receptor antagonist nizatidine (NZ) to nitrosation in simulated gastric juice (SGJ) and under WHO-suggested conditions was investigated for the first time. For monitoring the nitrosatability of NZ, a hydrophilic interaction liquid chromatography (HILIC) method was optimized and validated according to FDA guidance. A Cosmosil HILIC® column and a mobile phase composed of acetonitrile: 0.04 M acetate buffer pH 6.0 (92:8, v/v) were used for the separation of NZ and its N-nitroso derivative (NZ-NO) within 6 min with LODs of 0.02 and 0.1 μg/mL, respectively. NZ was found highly susceptible to nitrosation in SGJ reaching 100% nitrosation …

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Simultaneous Determination Of Cardiovascular Drugs In Dried Blood Spot By Liquid Chromatography-Tandem Mass Spectrometry, H.M. Kim, J.-H. Park, N.P. Long, D.-D. Kim, S.W. Kwon Jul 2020

Simultaneous Determination Of Cardiovascular Drugs In Dried Blood Spot By Liquid Chromatography-Tandem Mass Spectrometry, H.M. Kim, J.-H. Park, N.P. Long, D.-D. Kim, S.W. Kwon

Journal of Food and Drug Analysis

A dried blood spot (DBS) sampling method was exploited to extract cardiovascular drugs using a small volume of whole blood of human and rodent. Thereafter, an analytical method using liquid chromatography with tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of 12 cardiovascular drugs. A 6 mm internal diameter disc containing 10 μL of blood was punched from a specifically designed card and analyzed by LC-MS/MS using a gradient elution method with a total run time of 16 min. For sample separation, a universal octadecyl-silica column was used with a flow rate of 0.2 mL/min. The developed …

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Chemical Investigation Of Hyptis Suaveolens Seed, A Potential Antihyperuricemic Nutraceutical, With Assistance Of Hplc-Spe-Nmr, F.-C. Hsu, S.-F. Tsai, S.-S. Lee Jul 2020

Chemical Investigation Of Hyptis Suaveolens Seed, A Potential Antihyperuricemic Nutraceutical, With Assistance Of Hplc-Spe-Nmr, F.-C. Hsu, S.-F. Tsai, S.-S. Lee

Journal of Food and Drug Analysis

The seed of Hyptis suaveolens, commonly known as wild flour ball (san fen yuan) in Taiwan, serves as a main refreshing drink substance in several regions. This study investigated firstly its secondary metabolites, leading to the isolation of five major caffeoylquinic acid derivatives (1–5) from the ethanol extract. In addition, ten minors, including three caffeoylquinic acid derivatives (12–14), were characterized via assistance of HPLC-SPE-NMR. Of these isolates, sodium 4,5-dicaffeoylquinate (2) and methyl 3,5-dicaffeoylquinate (4) showed moderate inhibitory activity against xanthine oxidase with the respective IC50 values of 69.4 μM and 92.1 μM (c.f. allopurinol IC50 28.4 μM). Quantitative HPLC analysis …

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Quercetin Facilitates Cell Death And Chemosensitivity Through Rage/Pi3k/Akt/Mtor Axis In Human Pancreatic Cancer Cells, C.-Y. Lan, S.-Y. Chen, C.-W. Kuo, C.-C. Lu, G.-C. Yen Jul 2020

Quercetin Facilitates Cell Death And Chemosensitivity Through Rage/Pi3k/Akt/Mtor Axis In Human Pancreatic Cancer Cells, C.-Y. Lan, S.-Y. Chen, C.-W. Kuo, C.-C. Lu, G.-C. Yen

Journal of Food and Drug Analysis

The triggering of gemcitabine (GEM) drug resistance in pancreatic cancer by the receptor for advanced glycation end products (RAGE) has been demonstrated. Hence, finding a safe and effective adjuvant for preventing pancreatic cancer progression is imperative. Quercetin is a flavonoid that is abundant in apples, grapes, red raspberry, and onions and has been reported to inhibit RAGE. This research aimed to investigate the mechanisms of quercetin in regulating cell death and enhancing drug effects through RAGE reduction, especially in GEM-resistant pancreatic cancer cells. Our results showed that silencing RAGE expression by RAGE-specific siRNA transfection significantly increased cell death by apoptosis, …

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A Low Cost Smart System To Analyze Different Types Of Edible Bird's Nest Adulteration Based On Colorimetric Sensor Array, X. Huang, Z. Li, X. Zou, J. Shi, Tahir H. Elrasheid, Y. Xu, X. Zhai, X. Hu Jul 2020

A Low Cost Smart System To Analyze Different Types Of Edible Bird's Nest Adulteration Based On Colorimetric Sensor Array, X. Huang, Z. Li, X. Zou, J. Shi, Tahir H. Elrasheid, Y. Xu, X. Zhai, X. Hu

Journal of Food and Drug Analysis

This study was performed to develop a low-cost smart system for identification and quantification of adulterated edible bird's nest (EBN). The smart system was constructed with a colorimetric sensor array (CSA), a smartphone and a multi-layered network model. The CSA were used to collect the odor character of EBN and the response signals of CSA were captured by the smartphone systems. The principal component analysis (PCA) and hierarchical cluster analysis (HAC) were used to inquiry the similarity among authentic and adulterated EBNs. The multi-layered network model was constructed to analyze EBN adulteration. In this model, discrimination of authentic EBN and …

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Impact Of Dietary Ingredients On The Interpretation Of Various Fecal Parameters In Rats Fed Inulin, H.-J. Chen, F.-J. Dai, C.-R. Chang, Y.-Q. Lau, B.-S. Chew, C.-F. Chau Jul 2020

Impact Of Dietary Ingredients On The Interpretation Of Various Fecal Parameters In Rats Fed Inulin, H.-J. Chen, F.-J. Dai, C.-R. Chang, Y.-Q. Lau, B.-S. Chew, C.-F. Chau

Journal of Food and Drug Analysis

In the present study, the influences of diets (i.e. chow and AIN-93 diets) on the interpretation of various fecal parameters including viable microbiota, moisture, weight, and short-chain fatty acids in rats fed different amounts of inulin (0.5–2 g/kg). Eight groups of rats (n = 8/group) were fed, for 4 weeks, chow or AIN-93 diets with or without inulin supplementation. Fecal samples were analyzed for different fecal parameters. After a 2-week adaptation, apparent differences in some fecal parameters were observed between the chow and AIN-93 diet groups. Throughout the 4-week intervention period, significantly (p < 0.05) higher Lactobacillus spp. counts, fecal moisture (∼2.7-fold), and fecal weight (∼5.8-fold) were observed with chow diet over AIN-93 diet. More specifically, significant elevations in the levels of Bifidobacterium spp., Lactobacillus spp., fecal moisture, and fecal weight could be observed at low-dose (0.5 g/kg) of inulin in chow diet groups, while most of these changes could merely be seen at medium-dose (1 g/kg) in AIN-93 diet groups. These results demonstrated that the choice of experimental diets would affect the comparison of fecal parameters as well as the interpretation of effective dosage of prebiotic in intestinal health assessments. © 2019

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Efficient Identification Of Fungal Antimicrobial Principles By Tandem Ms And Nmr Database, M.-S. Lee, Y.-L. Yang, C.-Y. Wu, Y.-L. Chen, C.-K. Lee, S.-S. Tzean, T.-H. Lee Jul 2020

Efficient Identification Of Fungal Antimicrobial Principles By Tandem Ms And Nmr Database, M.-S. Lee, Y.-L. Yang, C.-Y. Wu, Y.-L. Chen, C.-K. Lee, S.-S. Tzean, T.-H. Lee

Journal of Food and Drug Analysis

The continuous re-isolation of the known and non-applicable compounds that is time-consuming and wasting resources is still a critical problem in the discovery of bioactive entities from natural resources. To efficiently address the problem, high performance liquid chromatography-diode array detector-microfractionation (HPLC-DAD-microfractionation) guided by disk agar diffusion assay was developed, and the active compounds were further identified using the tandem mass spectrometry (MS/MS)-based molecular networking. Of 150 fungal strains screened, the methanolic extracts of Phoma herbarum PPM7487, Cryptosporiopsis ericae PPM7405, and Albifimbria verrucaria PPM945 exhibited potent antimicrobial activity against Candida albicans SC5314 and Cryptococcus neoformans H99 in the preliminary agar diffusion …

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