Open Access. Powered by Scholars. Published by Universities.®

Medicinal Chemistry and Pharmaceutics Commons™

Open Access. Powered by Scholars. Published by Universities.®

Toxicology

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 871 - 900 of 1074

Full-Text Articles in Medicinal Chemistry and Pharmaceutics

Composition Of Phenolic Compounds And Antioxidant Attributes Of Cyclea Gracillima Diels Extracts, J.-T. Lin, S.-C. Liu, L.-C. Kuo, D.-J. Yang Jul 2020

Composition Of Phenolic Compounds And Antioxidant Attributes Of Cyclea Gracillima Diels Extracts, J.-T. Lin, S.-C. Liu, L.-C. Kuo, D.-J. Yang

Journal of Food and Drug Analysis

Cyclea gracillima Diels is a Taiwanese native medicinal herb. However, there are currently few relevant reports on its biochemical activity. In this study, the antioxidant attributes of the ethanol and hot water extracts of this herb were assayed using in vitro models, including the following: 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazyl radical scavenging, Trolox equivalent antioxidant capacity, reducing power, and chelating ferrous ions. The following biochemical models were also assayed: inhibition of human low density lipoprotein oxidation, inhibition of human erythrocyte hemolysis, and scavenging oxygen radicals in human blood. The composition and content of flavonoids and phenolic acids in these extracts were also analyzed. The …

Go to article

Anti-Inflammatory Effect Of Cinnamaldehyde And Linalool From The Leaf Essential Oil Of Cinnamomum Osmophloeum Kanehira In Endotoxin-Induced Mice, S.-C. Lee, S.-Y. Wang, C.-C. Li, C.-T. Liu Jul 2020

Anti-Inflammatory Effect Of Cinnamaldehyde And Linalool From The Leaf Essential Oil Of Cinnamomum Osmophloeum Kanehira In Endotoxin-Induced Mice, S.-C. Lee, S.-Y. Wang, C.-C. Li, C.-T. Liu

Journal of Food and Drug Analysis

Cinnamomum osmophloeum Kanehira is a Taiwan native plant that belongs to genus Cinnamomum and is also known as pseudocinnamomum or indigenous cinnamon. Its leaf is traditionally used by local people in cooking and as folk therapy. We previously demonstrated the chemical composition and anti-inflammatory effect of leaf essential oil of Cinnamomum osmophloeum Kanehira of linalool chemotype in streptozotocin-induced diabetic rats and on endotoxin-injected mice. The aim of the present study is to evaluate whether cinnamaldehyde and linalool the active anti-inflammatory compounds in leaf essential oil of Cinnamomum osmophloeum Kanehira. Before the injection of endotoxin, C57BL/6 mice of the experimental groups …

Go to article

Selected Essential Oils Inhibit Key Physiological Enzymes And Possess Intracellular And Extracellular Antimelanogenic Properties In Vitro, Z. Aumeeruddy-Elalfi, N. Lall, B. Fibrich, A. Blom Van Staden, M. Hosenally, M.F. Mahomoodally Jul 2020

Selected Essential Oils Inhibit Key Physiological Enzymes And Possess Intracellular And Extracellular Antimelanogenic Properties In Vitro, Z. Aumeeruddy-Elalfi, N. Lall, B. Fibrich, A. Blom Van Staden, M. Hosenally, M.F. Mahomoodally

Journal of Food and Drug Analysis

Essential oils (EOs) extracted from six medicinal herbs and food plants [Cinnamomum zeylanicum (CZ), Psiadia arguta (PA), Psiadia terebinthina (PT), Citrus grandis (CGp), Citrus hystrix (CH), and Citrus reticulata (CR)] were studied for any inhibitory potential against key physiological enzymes involved in diabetes (α-glucosidase), skin aging (collagenase and elastase), and neurodegenerative disorders (acetylcholinesterase). Kinetic studies of the active EOs on the aforementioned enzymes were determined using Lineweaver–Burk plots. The intracellular and extracellular antimelanogenic potential of the EOs were evaluated on B16F10 mouse melanocytes. CH and CR were found to significantly inhibit (2.476 ± 0.13 μg/mL and 3.636 ± 0.10 μg/mL, …

Go to article

Characterization Of An Extracellular Β-Glucosidase From Dekkera Bruxellensis For Resveratrol Production, H.-P. Kuo, R. Wang, C.-Y. Huang, J.-T. Lai, Y.-C. Lo, S.-T. Huang Jul 2020

Characterization Of An Extracellular Β-Glucosidase From Dekkera Bruxellensis For Resveratrol Production, H.-P. Kuo, R. Wang, C.-Y. Huang, J.-T. Lai, Y.-C. Lo, S.-T. Huang

Journal of Food and Drug Analysis

Polygonum cuspidatum is a widely grown crop with a rich source of polydatin (also called piceid) for resveratrol production. Resveratrol is produced from piceid via enzymatic cleavage of the sugar moiety of piceid. In this study, Dekkera bruxellensis mutants were selected based on their high p-nitrophenyl-β-D-glucopyranoside and piceid conversion activities. The enzyme responsible for piceid conversion was a heterodimeric protein complex that was predominantly secreted to the extracellular medium and consisted of two subunits at an equal ratio with molecular masses of 30.5 kDa and 48.3 kDa. The two subunits were identified as SCW4p and glucan-β-glucosidase precursor in D. bruxellensis. …

Go to article

A Novel Poly (Glycine) Biosensor Towards The Detection Of Indigo Carmine: A Voltammetric Study, J.G.G. Manjunatha Jul 2020

A Novel Poly (Glycine) Biosensor Towards The Detection Of Indigo Carmine: A Voltammetric Study, J.G.G. Manjunatha

Journal of Food and Drug Analysis

The electrochemical behavior of indigo carmine (IC) at poly (glycine) modified carbon paste electrode (PGMCPE) was investigated by cyclic and differential pulse voltammetry. The oxidation peak of IC was observed in phosphate buffer of pH 6.5. The influence of different pH, scan rate, and concentration were analyzed. The probable reaction mechanism involved in the oxidation of IC was also proposed. Results showed that PGMCPE a remarkable electrocatalytic activity for the oxidation of IC under optimal conditions. The electrocatalytic response of the sensor was proportional to the IC concentration in the range of (2 × 10 −6 –1 × 10 −5 …

Go to article

Codissolution Of Calcium Hydrogenphosphate And Sodium Hydrogencitrate In Water. Spontaneous Supersaturation Of Calcium Citrate Increasing Calcium Bioavailability, M. Vavrusova, B.P. Danielsen, A.C. Garcia, L.H. Skibsted Jul 2020

Codissolution Of Calcium Hydrogenphosphate And Sodium Hydrogencitrate In Water. Spontaneous Supersaturation Of Calcium Citrate Increasing Calcium Bioavailability, M. Vavrusova, B.P. Danielsen, A.C. Garcia, L.H. Skibsted

Journal of Food and Drug Analysis

The sparingly soluble calcium hydrogenphosphate dihydrate, co-dissolving in water during dissolution of freely soluble sodium hydrogencitrate sesquihydrate as caused by proton transfer from hydrogencitrate to hydrogenphosphate, was found to form homogenous solutions supersaturated by a factor up to 8 in calcium citrate tetrahydrate. A critical hydrogencitrate concentration for formation of homogeneous solutions was found to depend linearly on dissolved calcium hydrogenphosphate: [HCitr 2− ] = 14[CaHPO 4 ] - 0.05 at 25 °C. The lag phase for precipitation of calcium citrate tetrahydrate, as identified from FT-IR spectra, from these spontaneously formed supersaturated solutions was several hours, and the time to …

Go to article

Hepatoprotective Effect Of The Ethanol Extract Of Polygonum Orientale On Carbon Tetrachloride-Induced Acute Liver Injury In Mice, Y.-J. Chiu, S.-C. Chou, C.-S. Chiu, C.-P. Kao, K.-C. Wu, C.-J. Chen, J.-C. Tsai, W.-H. Peng Jul 2020

Hepatoprotective Effect Of The Ethanol Extract Of Polygonum Orientale On Carbon Tetrachloride-Induced Acute Liver Injury In Mice, Y.-J. Chiu, S.-C. Chou, C.-S. Chiu, C.-P. Kao, K.-C. Wu, C.-J. Chen, J.-C. Tsai, W.-H. Peng

Journal of Food and Drug Analysis

Polygonum orientale L. (Polygonaceae) fruits have various medicinal uses, but their hepatoprotective effects have not yet been studied. This study investigated the hepatoprotective activity of the ethanolic extract of P. orientale (POE) fruits against carbon tetrachloride (CCl 4 )-induced acute liver injury (ALI). Mice were pretreated with POE (0.1, 0.5, and 1.0 g/kg) or silymarin (0.2 g/kg) for 5 consecutive days and administered a dose of 0.175% CCl 4 (ip) on the 5th day to induce ALI. Blood and liver samples were collected to measure antioxidative activity and cytokines. The bioactive components of POE were identified through high-performance liquid chromatography …

Go to article

Non-Destructive Profiling Of Volatile Organic Compounds Using Hs-Spme/Gc–Ms And Its Application For The Geographical Discrimination Of White Rice, D.K. Lim, C. Mo, D.-K. Lee, N.P. Long, J. Lim, S.W. Kwon Jul 2020

Non-Destructive Profiling Of Volatile Organic Compounds Using Hs-Spme/Gc–Ms And Its Application For The Geographical Discrimination Of White Rice, D.K. Lim, C. Mo, D.-K. Lee, N.P. Long, J. Lim, S.W. Kwon

Journal of Food and Drug Analysis

The authenticity determination of white rice is crucial to prevent deceptive origin labeling and dishonest trading. However, a non-destructive and comprehensive method for rapidly discriminating the geographical origins of white rice between countries is still lacking. In the current study, we developed a volatile organic compound based geographical discrimination method using headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME/GC–MS) to discriminate rice samples from Korea and China. A partial least squares discriminant analysis (PLS-DA) model exhibited a good classification of white rice between Korea and China (accuracy = 0.958, goodness of fit = 0.937, goodness of prediction = 0.831, …

Go to article

A Randomized, Double-Blind Clinical Study Of The Effects Of Ankascin 568 Plus On Blood Lipid Regulation, S.-F. Liu, Y.-R. Wang, Y.-C. Shen, C.-L. Chen, C.-N. Huang, T.-M. Pan, C.-K. Wang Jul 2020

A Randomized, Double-Blind Clinical Study Of The Effects Of Ankascin 568 Plus On Blood Lipid Regulation, S.-F. Liu, Y.-R. Wang, Y.-C. Shen, C.-L. Chen, C.-N. Huang, T.-M. Pan, C.-K. Wang

Journal of Food and Drug Analysis

Hyperlipidemia and inflammation play important roles in the development and progression of atherosclerosis. Atherosclerosis is regarded as an inflammatory response of blood vessels to injury at the start of atherosclerotic plaque formation, which then leads to cardiovascular events. Edible fungi of the Monascus species have been used as traditional Chinese medicines in East Asia for several centuries. The fermented products of Monascus purpureus NTU 568 possess a number of functional secondary metabolites including the anti-inflammatory pigments monascin and ankaflavin. Compounds derived from M. purpureus have been shown to have hypolipidemic effects. We aimed to evaluate the effects of M. purpureus …

Go to article

Efficacy Of Protein Rich Pearl Powder On Antioxidant Status In A Randomized Placebo-Controlled Trial, H.-F. Chiu, S.-C. Hsiao, Y.-Y. Lu, Y.-C. Han, Y.-C. Shen, K. Venkatakrishnan, C.-K. Wang Jul 2020

Efficacy Of Protein Rich Pearl Powder On Antioxidant Status In A Randomized Placebo-Controlled Trial, H.-F. Chiu, S.-C. Hsiao, Y.-Y. Lu, Y.-C. Han, Y.-C. Shen, K. Venkatakrishnan, C.-K. Wang

Journal of Food and Drug Analysis

Pearl is one of the well-known traditional Chinese medicine (TCM) prescribed for treating various skin and bone related disorders due to its abundant proteins and mineral contents. The present investigation focused on antioxidation and life span prolonging effects from different extracts of pearl powder. During in vitro studies, various oxidative indices were evaluated, along with lifespan-prolonging effect were checked using wild-type Caenorhabditis elegans. For the clinical trial, 20 healthy middle-aged subjects were recruited and separated into 2 groups as experimental and placebo group, who received 3 g of pearl powder/d (n = 10) and 3 g of placebo/d (n = …

Go to article

Rapid Characterization Of Metabolites In Soybean Using Ultra High Performance Liquid Chromatography Coupled With Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (Uplc-Esi-Q-Tof-Ms/Ms) And Screening For Α-Glucosidase Inhibitory And Antioxidant Properties Through Different Solvent Systems, Y.H. Lee, B. Kim, S.-R. Hwang, K. Kim, J.H. Lee Jul 2020

Rapid Characterization Of Metabolites In Soybean Using Ultra High Performance Liquid Chromatography Coupled With Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (Uplc-Esi-Q-Tof-Ms/Ms) And Screening For Α-Glucosidase Inhibitory And Antioxidant Properties Through Different Solvent Systems, Y.H. Lee, B. Kim, S.-R. Hwang, K. Kim, J.H. Lee

Journal of Food and Drug Analysis

This work was the first to investigate on the simultaneous characterization of metabolite profiles in soybean using UPLC-ESI-Q-TOF-MS/MS. Twenty two compositions were observed within 14 min from the methanol extract and confirmed as twelve isoflavones of three types and ten soyasaponins (Ab, Af, I–III, αg, βg, βa, γg, and γa). Moreover, the patterns of two chemicals showed considerable differences in seven solvent systems by HPLC analysis and their optimal extraction was achieved by 70% methanol (isoflavone: 4102.69 μg/g; soyasaponin: ten peaks). The second abundant isoflavones were detected in 50% methanol (4054.39 μg/g), followed by 30% methanol, 100% methanol, 10% methanol, …

Go to article

Detection Of Lithospermate B In Rat Plasma At The Nanogram Level By Lc/Ms In Multi Reaction Monitoring Mode, T.-Y. Chung, N.-H. Lin, Y.-C. Li, T.-Y. Chen, P.-C. Kuo, W.-Y. Chen, J.T.C. Tzen Jul 2020

Detection Of Lithospermate B In Rat Plasma At The Nanogram Level By Lc/Ms In Multi Reaction Monitoring Mode, T.-Y. Chung, N.-H. Lin, Y.-C. Li, T.-Y. Chen, P.-C. Kuo, W.-Y. Chen, J.T.C. Tzen

Journal of Food and Drug Analysis

Low bioavailability and high binding affinity to plasma proteins led to the difficulty for the quantitative detection of lithospermate B (LSB) in plasma. This study aimed to develop a protocol for detecting LSB in plasma. A method was employed to quantitatively detect LSB of 5–500 ng/mL by LC/MS spectrometry in multi reaction monitoring mode via monitoring two major fragments with m/z values of 519 and 321 in the MS2 spectrum. To set up an adequate extraction solution to release LSB captured by plasma proteins, recovery yields of LSB extracted from rat plasma acidified by formic acid or HCl in the …

Go to article

Study Of Forced Degradation Behavior Of Pramlintide Acetate By Hplc And Lc–Ms, Y. Yuan, Y.-B. Li, Z.-F. Tai, Y.-P. Xie, X.-F. Pu, J. Gao Jul 2020

Study Of Forced Degradation Behavior Of Pramlintide Acetate By Hplc And Lc–Ms, Y. Yuan, Y.-B. Li, Z.-F. Tai, Y.-P. Xie, X.-F. Pu, J. Gao

Journal of Food and Drug Analysis

Pramlintide acetate (Symlin ® ), a synthetic analogue of the human hormone amylin. It was approved in March 2005 as a subcutaneous injection for the adjunctive treatment of patients who have type 1 or 2 diabetes mellitus. The objective of current investigation was to study the degradation behavior of pramlintide acetate under different ICH recommended stress conditions by HPLC and LC–MS. Pramlintide acetate was subjected to stress conditions of hydrolysis (acidic or alkaline), oxidation, photolysis and thermal decomposition. Extensive degradation products were observed under the hydrolysis, oxidation or thermal stress conditions, while minimal degradation was found in the photolytic conditions. …

Go to article

Methods For Efficient Analysis Of Tocopherols, Tocotrienols And Their Metabolites In Animal Samples With Hplc-Ec, M.-J. Lee, W. Feng, L. Yang, Y.-K. Chen, E. Chi, A. Liu, C.S. Yang Jul 2020

Methods For Efficient Analysis Of Tocopherols, Tocotrienols And Their Metabolites In Animal Samples With Hplc-Ec, M.-J. Lee, W. Feng, L. Yang, Y.-K. Chen, E. Chi, A. Liu, C.S. Yang

Journal of Food and Drug Analysis

Tocopherols and tocotrienols, collectively known as vitamin E, have received a great deal of attention because of their interesting biological activities. In the present study, we reexamined and improved previous methods of sample preparation and the conditions of high-performance liquid chromatography for more accurate quantification of tocopherols, tocotrienols and their major chain-degradation metabolites. For the analysis of serum tocopherols/tocotrienols, we reconfirmed our method of mixing serum with ethanol followed by hexane extraction. For the analysis of tissue samples, we improved our methods by extracting tocopherols/tocotrienols directly from tissue homogenate with hexane. For the analysis of total amounts (conjugated and unconjugated …

Go to article

Use Of A Bayesian Approach In The Design And Evaluation Of Nce2s, C.-Y. Wang, L.-C. Chang, M.-S. Lin, C.-F. Hsiao, J.-D. Huang Jul 2020

Use Of A Bayesian Approach In The Design And Evaluation Of Nce2s, C.-Y. Wang, L.-C. Chang, M.-S. Lin, C.-F. Hsiao, J.-D. Huang

Journal of Food and Drug Analysis

Taiwan's regulatory agency defines New Chemical Entity 2 (NCE2) as a compound drug that has been approved and marketed for ten years in a top-ten pharmaceutically-advanced country but which is new in Taiwan. To apply for registration of NCE2 in Taiwan, a clinical trial may be conducted in Taiwan to evaluate the efficacy and safety. Since the NCE2 has been approved in at least one of the top-ten pharmaceutically-advanced countries, we can borrow the information from all of the observed data from other countries to synthesize the data from both Taiwan and other countries to assess the NCE2 efficacy. In …

Go to article

Stem Cell Therapy On Skin: Mechanisms, Recent Advances And Drug Reviewing Issues, G.-Y. Chu, Y.-F. Chen, H.-Y. Chen, M.-H. Chan, C.-S. Gau, S.-M. Weng Jul 2020

Stem Cell Therapy On Skin: Mechanisms, Recent Advances And Drug Reviewing Issues, G.-Y. Chu, Y.-F. Chen, H.-Y. Chen, M.-H. Chan, C.-S. Gau, S.-M. Weng

Journal of Food and Drug Analysis

Stem cell products and its clinical applications have been widely discussed in recent years, particularly when the Japanese “induced pluripotent stem cells” founder Dr. Yamanaka was awarded as Nobel Prize laureate in 2013. For decades, major progresses have been achieved in the stem cell biology field, and more and more evidence showed that skin stem cells are involved in the process of skin repair. Stem/progenitor cells of the epidermis are recognized to play the most essential role in the tissue regeneration of skin. In this review, we first illustrated basic stem cell characteristics and various stem cell subtypes resided in …

Go to article

Development And Validation Of An Lc–Ms/Ms Method For Quantification Of Nc-8 In Rat Plasma And Its Application To Pharmacokinetic Studies, B.H. Kachingwe, Y.-S. Uang, T.-J. Huang, L.-H. Wang, S.-J. Lin Jul 2020

Development And Validation Of An Lc–Ms/Ms Method For Quantification Of Nc-8 In Rat Plasma And Its Application To Pharmacokinetic Studies, B.H. Kachingwe, Y.-S. Uang, T.-J. Huang, L.-H. Wang, S.-J. Lin

Journal of Food and Drug Analysis

ent-16-Oxobeyeran-19-N-methylureido (NC-8) is a recently synthesized derivative of isosteviol that showed anti-hepatitis B virus (HBV) activity by disturbing replication and gene expression of the HBV and by inhibiting the host toll-like receptor 2/nuclear factor-κB signaling pathway. To study its pharmacokinetics as a part of the drug development process, a highly sensitive, rapid, and reliable liquid chromatography tandem mass spectrometry (LC–MS/MS) method was developed and validated for determining NC-8 in rat plasma. After protein precipitation extraction, the chromatographic separation of the analyte and internal standard (IS; diclofenac sodium) was performed on a reverse-phase Luna C18 column coupled with a Quattro Ultima …

Go to article

Studies On Prevention Of Obesity, Metabolic Syndrome, Diabetes, Cardiovascular Diseases And Cancer By Tea, C.S. Yang, H. Wang, Z.P. Sheridan Jul 2020

Studies On Prevention Of Obesity, Metabolic Syndrome, Diabetes, Cardiovascular Diseases And Cancer By Tea, C.S. Yang, H. Wang, Z.P. Sheridan

Journal of Food and Drug Analysis

Tea, a popular beverage made from leaves of the plant Camellia sinensis, has been studied extensively in recent decades for its beneficial health effects in the prevention of obesity, metabolic syndrome, diabetes, cancer, and other diseases. Whereas these beneficial effects have been convincingly demonstrated in most laboratory studies, results from human studies have not been consistent. Some studies demonstrated that weight reduction, alleviation of metabolic syndrome and risk reduction in diabetes were only observed in individuals who consume 3–4 cups of tea (600–900 mg tea catechins) or more daily. This chapter reviews some of these studies, the possible mechanisms of …

Go to article

A Novel Protein From Edible Fungi Cordyceps Militaris That Induces Apoptosis, K.-C. Bai, F. Sheu Jul 2020

A Novel Protein From Edible Fungi Cordyceps Militaris That Induces Apoptosis, K.-C. Bai, F. Sheu

Journal of Food and Drug Analysis

Cordyceps militaris is a dietary therapeutic fungus that is an important model species in Cordyceps research. In this study, we purified a novel protein from the fruit bodies of C. militaris and designated it as Cordyceps militaris protein (CMP). CMP has a molecular mass of 18.0 kDa and is not glycosylated. Interestingly, CMP inhibited cell viability in murine primary cells and other cell lines in a time- and dose-dependent manner. Using trypan blue staining and a lactate dehydrogenase release assay, we showed that CMP caused cell death in the murine hepatoma cell line BNL 1MEA.7R.1. Furthermore, the frequency of BNL …

Go to article

Corrigendum To “Investigation Of Borneols Sold In Taiwan By Chiral Gas Chromatography” (Journal Of Food And Drug Analysis (2018) 26(1) (348–352), (S1021949816301612) (10.1016/J.Jfda.2016.10.012)), T.-J. Ho, C.-C. Hung, T.-L. Shih, L.-M. Yiin, H.-P. Chen Jul 2020

Corrigendum To “Investigation Of Borneols Sold In Taiwan By Chiral Gas Chromatography” (Journal Of Food And Drug Analysis (2018) 26(1) (348–352), (S1021949816301612) (10.1016/J.Jfda.2016.10.012)), T.-J. Ho, C.-C. Hung, T.-L. Shih, L.-M. Yiin, H.-P. Chen

Journal of Food and Drug Analysis

Full Text: 1. In Graphic abstract, Figure 4 and Table 2, the labels for (+)-isoborneol and (−)-isoborneol should be switched. 2. 1. In Figure 1, the labels for d-isoborneol and l-isoborneol should be switched. 3. On Page 349, in Figure 2, the label for (+)-isoborneol should be (−)-isoborneol. 4. On Page 350, in Section 3.1, the first line of the second paragraph, “(+)-Isoborneol, synthesized as previously described …. ” should be “(−)-Isoborneol, synthesized as previously described …. ”. 5. On Page 351, in the legend for Figure 4, the order of (+)-isoborneol, (−)-isoborneol should be switched. © 2018

Go to article

Therapeutic Effects Of Lactobacillus Paracasei Subsp. Paracasei Ntu 101 Powder On Dextran Sulfate Sodium-Induced Colitis In Mice, C.-L. Chen, P.-Y. Hsu, T.-M. Pan Jul 2020

Therapeutic Effects Of Lactobacillus Paracasei Subsp. Paracasei Ntu 101 Powder On Dextran Sulfate Sodium-Induced Colitis In Mice, C.-L. Chen, P.-Y. Hsu, T.-M. Pan

Journal of Food and Drug Analysis

Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) whose exact cause is still unclear. Disruption of the intestinal microflora is considered one of the main causes of the disease. Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) is a multifunctional strain that has been shown in previous studies to possess anti-inflammatory properties and to exert a modulatory effect on intestinal bacteria associated with certain pathogenic mechanisms of IBD. In the current study, we investigated the effects of NTU 101 on dextran sulfate sodium (DSS)-induced colitis in a mouse model. Colitis was induced in male C57BL/6 mice (total …

Go to article

Down-Regulation Of Partial Substitution For Staple Food By Oat Noodles On Blood Lipid Levels: A Randomized, Double-Blind, Clinical Trial, M.-Y. Liao, Y.-C. Shen, H.-F. Chiu, S.-M. Ten, Y.-Y. Lu, Y.-C. Han, K. Venkatakrishnan, S.-F. Yang, C.-K. Wang Jul 2020

Down-Regulation Of Partial Substitution For Staple Food By Oat Noodles On Blood Lipid Levels: A Randomized, Double-Blind, Clinical Trial, M.-Y. Liao, Y.-C. Shen, H.-F. Chiu, S.-M. Ten, Y.-Y. Lu, Y.-C. Han, K. Venkatakrishnan, S.-F. Yang, C.-K. Wang

Journal of Food and Drug Analysis

This clinical trial was conducted to assess the lipid-lowering activity of oat noodles by replacing partial staple food (wheat or rice noodle) in normal and mildly hypercholesterolemic subjects. Totally 84 healthy and mild hypercholesterolemic subjects were recruited and divided into 2 groups as experimental (oat noodles) and placebo (wheat noodles) and instructed to consume 100 g of oat noodles or wheat noodles (replacing one or two meals/day) for 10 weeks and followed by 2 weeks of follow up (without noodle consumption). Various anthropometric measurements and biochemical analysis were carried out during initial (baseline), 2nd, 6th, 10th and 12th week (follow-up). …

Go to article

A Steroidal Derivative From Trigonella Foenum Graecum L. That Induces Apoptosis In Vitro And In Vivo, R.S. Thakur, B. Ahirwar Jul 2020

A Steroidal Derivative From Trigonella Foenum Graecum L. That Induces Apoptosis In Vitro And In Vivo, R.S. Thakur, B. Ahirwar

Journal of Food and Drug Analysis

Trigonella foenum graecum L. is a dietary herb used in traditional medicine system. In this study, we investigated the cytotoxicity, antitumor, antimetastatic and antiangiogenic effect of the steroidal compound, ethyl iso-allocholate isolated from T. foenum graecum L. seeds against A549 lung cancer cells in vitro and in vivo. Among all the isolated compounds, the ethyl iso-allocholate rendered the highest cytotoxicity potential. It showed least percentage cell viability in trypan blue assay and lowest nuclei count in hoechst staining. The caspase glo assay and western blot analysis showed the significant caspase 3 cleavage, indicating caspase dependent apoptosis. Consistent with the in …

Go to article

Multivariate Optimization Of Mebeverine Analysis Using Molecularly Imprinted Polymer Electrochemical Sensor Based On Silver Nanoparticles, A. Nezhadali, G.A. Bonakdar Jul 2020

Multivariate Optimization Of Mebeverine Analysis Using Molecularly Imprinted Polymer Electrochemical Sensor Based On Silver Nanoparticles, A. Nezhadali, G.A. Bonakdar

Journal of Food and Drug Analysis

Thin film of a moleculary imprinted polymer (MIP) based on electropolymerization method with sensitive and selective binding sites for mebeverine (MEB) was developed. This film was cast on pencil graphite electrode (PGE) by electrochemical polymerization in solution of pyrrole (PY) and template MEB via cyclic voltammetry scans and further electrodeposition of silver nanoparticles (AgNPs). Several parameters controlling the performance of the silver nano particles MIP pencil graphite electrode (AgNPs-MIP-PGE) including concentration of PY(mM) concentration of mebeverine (mM), number of cycles in electropolymerization, scan rate of CV process (mV. s−1), deposition time of AgNPs on to the MIP surface (s), stirring …

Go to article

Isoprenyl Phenolic Ethers From The Termite Nest-Derived Medicinal Fungus Xylaria Fimbriata, M.-C. Chen, G.-J. Wang, Y.-H. Kuo, Y.-R. Chiang, T.-Y. Cho, Y.-M. Ju, T.-H. Lee Jul 2020

Isoprenyl Phenolic Ethers From The Termite Nest-Derived Medicinal Fungus Xylaria Fimbriata, M.-C. Chen, G.-J. Wang, Y.-H. Kuo, Y.-R. Chiang, T.-Y. Cho, Y.-M. Ju, T.-H. Lee

Journal of Food and Drug Analysis

Seven new isoprenyl phenolic ethers, namely fimbriethers A‒G (1‒7), were isolated from the fermented broth of the termite nest-derived medicinal fungus Xylaria fimbriata YMJ491. Their structures were determined by spectroscopic data analysis and compared with those reported. The effects of all the isolates at a concentration of 100 μM on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells were evaluated, and all of them exhibited NO production inhibitory activity with Emax values ranging from 4.6 ± 2.0% to 49.7 ± 0.5% without significant cytotoxicity. In addition, these seven compounds did not alter phenylephrine-induced …

Go to article

Hantzsch Pre-Column Derivatization For Simultaneous Determination Of Alendronate Sodium And Its Pharmacopoeial Related Impurity: Comparative Study With Synchronous Fluorometry Using Fluorescamine, A.F. El-Yazbi, E.I. El-Kimary, R.M. Youssef Jul 2020

Hantzsch Pre-Column Derivatization For Simultaneous Determination Of Alendronate Sodium And Its Pharmacopoeial Related Impurity: Comparative Study With Synchronous Fluorometry Using Fluorescamine, A.F. El-Yazbi, E.I. El-Kimary, R.M. Youssef

Journal of Food and Drug Analysis

High performance liquid chromatographic (HPLC) method with a pre-column derivatization based on Hantzsch condensation reaction was applied for simultaneous determination of alendronate sodium (ALN) and its main related impurity, 4-Aminobutanoic acid (ABA) at its pharmacopeial limit. The separation of colored condensation products of ALN and ABA were achieved on Agilent Zobrax Eclipse SB-C18 analytical column (250 × 4.6 mm, 5 μm) using a mobile phase composed of acetonitrile–0.1 M acetate buffer, pH 5.0 (15:85, v/v). The flow rate was 1 mL min−1. The detection was carried out at 340 nm using photo-diode array detector. Peak areas were used for the …

Go to article

In Vitro Α-Amylase And Pancreatic Lipase Inhibitory Activity Of Cornus Mas L. And Cornus Alba L. Fruit Extracts, A. Świerczewska, T. Buchholz, M.F. Melzig, M.E. Czerwińska Jul 2020

In Vitro Α-Amylase And Pancreatic Lipase Inhibitory Activity Of Cornus Mas L. And Cornus Alba L. Fruit Extracts, A. Świerczewska, T. Buchholz, M.F. Melzig, M.E. Czerwińska

Journal of Food and Drug Analysis

Less-common fruits from Cornus spp. (Cornaceae), also named dogwoods, have shown antidiabetic, antibacterial and anti-allergic properties and are thus considered a source of phytochemicals that are beneficial to human health. The study aimed to compare the chemical compositions of the aqueous and ethanolic extracts of lyophilized fresh-picked and commercially available dried fruits of Cornus mas (Cm, cornelian cherry) and Cornus alba (Ca) fruits using HPLC-DAD-MS/MS method. Simultaneously, the α-amylase and pancreatic lipase (PL) inhibitory activities of the prepared extracts were compared by in vitro fluorescence assay based on the kinetic hydrolysis of starch or oleate ester of 4-methylumbelliferone (MUO), respectively. …

Go to article

Comparative Study On Amount Of Nutraceuticals In By-Products From Solvent And Cold Pressing Methods Of Rice Bran Oil Processing, D. Wongwaiwech, M. Weerawatanakorn, S. Tharatha, C.-T. Ho Jul 2020

Comparative Study On Amount Of Nutraceuticals In By-Products From Solvent And Cold Pressing Methods Of Rice Bran Oil Processing, D. Wongwaiwech, M. Weerawatanakorn, S. Tharatha, C.-T. Ho

Journal of Food and Drug Analysis

Rice bran oil (RBO) has become a popular oil globally. However, the RBO extraction process leaves various residue products, which contain bioactive substances of varying potency which could be significant sources of functional ingredients for both food production and pharmaceutical manufacture. The objective of our study was to compare the bioactive substances in various by-products derived from the two rice bran oil processing methods; solvent extraction and cold pressing. The residues from solvent extraction processing contained up to 97.37 mg/100 g of γ-aminobutyric acid in defatted rice bran, and the rice acid oil contained high levels of vitamin E (tocopherols, …

Go to article

Raman Spectroscopy Coupled With Chemometric Methods For The Discrimination Of Foreign Fats And Oils In Cream And Yogurt, Karacaglar N.N. Yazgan, T. Bulat, I.H. Boyaci, A. Topcu Jul 2020

Raman Spectroscopy Coupled With Chemometric Methods For The Discrimination Of Foreign Fats And Oils In Cream And Yogurt, Karacaglar N.N. Yazgan, T. Bulat, I.H. Boyaci, A. Topcu

Journal of Food and Drug Analysis

The adulteration of milk fat in dairy products with cheaper non-milk based fats or oils is frequently encountered in the dairy industry. In this study, Raman spectroscopy with chemometric was used for the discrimination of foreign fats and oils in milk cream and yogurt. Firstly, binary mixtures of cream and oils (corn and sunflower oil), and vegetable fat blends which are potentially or currently used by the dairy industry were prepared. All fat or oil samples and their binary mixtures were examined by using Raman spectroscopy. Then, fat content of skim milk was adjusted to 3% (w/w) by the milk …

Go to article

Prioritization Of Pesticides In Crops With A Semi-Quantitative Risk Ranking Method For Taiwan Postmarket Monitoring Program, W.-C. Chou, W.-R. Tsai, H.-H. Chang, S.-Y. Lu, K.-F. Lin, P. Lin Jul 2020

Prioritization Of Pesticides In Crops With A Semi-Quantitative Risk Ranking Method For Taiwan Postmarket Monitoring Program, W.-C. Chou, W.-R. Tsai, H.-H. Chang, S.-Y. Lu, K.-F. Lin, P. Lin

Journal of Food and Drug Analysis

A risk-based prioritization of chemical hazards in monitoring programs allows regulatory agencies to focus on the most potentially concerned items involving human health risk. In this study, a risk-based matrix, with a scoring method using multiple factors for severity and probability of exposure, was employed to identify the pesticides presented in crops that may pose the greatest risk to human health. Both the probability of exposure and the severity were assessed for 91 pesticides detected in a Taiwanese postmarketing monitoring program. Probability of exposure was evaluated based on the probability of consumption and evidence of pesticide residues in crops. Severity …

Go to article

First Prev Next Last