Pharmacology, Toxicology and Environmental Health Commons^™

Editorial - Sure Journal Vol 6, Anne M. Friel, Eva Campion, Sinead Loughran

SURE Journal: Science Undergraduate Research Experience Journal

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Investigating Nekton Response To Changing Salinities In The Mississippi Sound: An Experimental And Statistical Approach, Adam Murray

Master's Theses

The Mississippi Sound provides nursery habitats for many coastal species and is recognized for its commercial fisheries. Previous freshening events linked to the Bonnet Carré Spillway, a Mississippi River flood diversion structure, proved catastrophic for oyster populations in the Mississippi Sound, but effects on mobile fish and shellfish species are not well-defined. The planned Mid-Breton Sediment Diversion (MBSD), an initiative to combat wetland loss, is also forecasted to lower salinities in the region, increasing the need to better understand responses of commercially and ecologically important species to freshening events. The objective of this study was to characterize effects of salinity …

Rabbit Control: Bait Products, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

This factsheet is part of the bait and poison directory for vertebrate pests in Western Australia (WA). It provides information on bait, poison, and toxin (bait) products available for use in WA to control rabbits (Oryctolagus cuniculus).

For information on how to access bait, poison and toxin (bait) products, training required, animal welfare considerations and legal requirements, refer to the Bait and poison directory for vertebrate pests’ page on the website at dpird.wa.gov.au. Information on bait, poison, and toxin (bait) products available for use in WA for rabbits, is provided below.

Rabbit Control: 1080 Versus Pindone, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

There are two toxins available for controlling pest rabbits in Western Australia: 1080 and pindone. This factsheet provides a brief summary of the main characteristics of both vertebrate pesticides. 1080 is generally the preferred toxin for rabbit control in Western Australia (WA).

Any attempt to reduce the impact of rabbits on the productivity of any agricultural enterprise (for example, pasture, crops, tree farms, horticulture) should be undertaken as part of a pest management plan.

This strategy should be proactive rather than reactive to a problem. It should also include a long term strategy for dealing with potential rabbit damage, including …

Rabbit Control: Fumigation, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

Fumigation for rabbit control involves the introduction of poisonous gas into a warren. It is one of the best control methods to use where low numbers of rabbits live in a few widely scattered warrens. The threat to non-target animals is minimal. This factsheet should be read in conjunction with registered product labels and directions for use. Check the Department of Health, Western Australia website (health.wa.gov.au) for public health requirements for the use of phosphine fumigation.

Fumigation may be used in areas where access is limited, such as fence lines, around trees, and on riverbanks, but it may be difficult …

Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan

DePaul Discoveries

Contamination of oceans by microplastics (mm) currently poses a major threat to aquatic and terrestrial ecosystems worldwide. Recent attention towards this issue has raised questions about the extent to which microplastics have accumulated in the environment, and has led to an increase in studies on the effects of microplastics in various organisms. However, levels of contamination in protected natural areas are still largely unexplored, yet can offer an important empirical perspective on the issue. In addition, little is known about the potential effects of microplastics on behavior in the field. This research was conducted within the protected ACE Basin National …

Toxicosis From Range Lupine Ingestion In A Research Herd Of Rafter 7 Merino Sheep, Benjamin J. Weigler, Walter F. Mandeville Iii, Scott Huber, Gary Mccuin, Fabrizio Caragena, Daniel D. Cook, Stephen T. Lee, Chris A. Pritsos

Poisonous Plant Research (PPR)

An outbreak of lupine alkaloid neurotoxicosis and death occurred in sheep grazed on rangeland areas of the Nevada Great Basin during the summer of 2023. Overall, 97 sheep died despite veterinary supportive care. Lupinus argenteus var. utahensis was implicated as the causative plant with high levels of lupanine (11.5 µg/mg) and spartiene (13.7 µg/mg) in collected and archived samples. Previous reports of sheep deaths due to lupine ingestion have not been recorded in this region. An increased abundance of the number plants producing seed pods having a high alkaloid content may have been associated with abnormally elevated levels of precipitation …

The Interaction Of Sodium Fluoride And Sodium Selenate On The Developmental Toxicity To Xenopus Laevis And Ambystoma Maculatum Embryos, Shelby Wolfram

Theses

Amphibians play critical roles in the environment's wellness. Fluoride (F) is a widely existing environmental pollutant. It is commonly known for being added to drinking water and as a topical dose on teeth. Selenium (Se) can be found in organic and inorganic forms in nature. Many different fertilizers, pesticides, insecticides, and fungicides contain sodium selenate in them. The range selenium has between deficiency and toxicity is narrow, meaning excess exposure could cause various adverse effects in aquatic organisms. Xenopus laevis is a standard model for developmental toxicity due to being minimal maintenance, cost efficient, and having transparent embryos. Xenopus makes …

The Effects Of Length, Weight, Age, And Gender On Mercury Concentrations In Burbot In North-Central Minnesota Lakes, Liam Hanley

Journal of Earth and Life Science

Burbot Lota lota in north-central Minnesota lakes have the potential to reach large sizes and consume large volumes of prey. This caveat may make burbot susceptible to higher rates of biocontamination, bioaccumulation and biomagnification. The objective of this study was to determine how changes in age, length, gender, weight, and lake affect total mercury concentrations in burbot. In this experiment 28 burbot were angled from three lakes: Cass (n = 17), Winnibigoshish (n = 4), and Bad Medicine (n = 7). Then tissue samples were taken from each fish and were lyophilized and homogenized. Homogenized tissue samples were analyzed by …

Molecular Investigation Of Tick-Borne Pathogens In Feral Swine (Sus Scrofa) From Seven Us States, Sofiane Aiche

Masters Theses

Feral swine (Sus scrofa) have been proposed as part of the lifecycles of multiple tickborne pathogens (TBP). This is a result of the increase in feral swine populations and increased tick populations and geographic ranges. Few surveys exist on the TBP relevant to human and wildlife of feral swine in the US. The aim of this study is to investigate the prevalence of 10 TBP: Anaplasma spp., Borrelia spp., Cytauxzoon felis, Hepatozoon spp., Babesia spp., Mycoplasma spp., Trypanosoma cruzi, Toxoplasma gondii, Yersinia pestis, and Rickettsia spp. A total of 87 feral swine blood samples …

Aversion Properties Of Retrorsine, Retrorsine Nitrogen Oxide And Frosted Senecio Latifolius, Leendert D. Snyman

Poisonous Plant Research (PPR)

Two pilot trials, investigating the aversive properties of retrorsine and retrorsine nitrogen oxide and that of frosted Senecio latifolius, were executed. Retrorsine and retrorsine-NO (the toxic principles of Senecio retrorsus), unlke sceleratine-NO (the toxic principle of another hepatotoxic pyrrolizidine alkaloid containing plant, namely S. latifolius), were not capable of establishing aversion when given to a sheep. Frosted S. latifolius given to a sheep also did not induce aversion. However, a sheep averted to S. latifolius (non-frosted) refused eating frosted S. latifolius.

Sceleratin Nitrogen Oxide As Aversive Agent In Conditioning Livestock To Avoid Senecio Latifolius, Leendert D. Snyman

Poisonous Plant Research (PPR)

Sceleratine nitrogen oxide, when administered together with a dichloromethane extract of Senecio latifolius, successfully conditioned cattle and sheep to avoid milled freeze dried S. latifolius mixed with maize meal. This treatment was effectively applied in conditioning steers to refuse eating S. latifolius grown in pots.

Isolation Of The Toxic Principle Of Senecio Latifolius By Means Of The Sensory Receptors Of Sheep, Leendert D. Snyman

Poisonous Plant Research (PPR)

The aversive substance of Senecio latifolius was isolated by means of the sensory receptors of sheep averted to S. latifolius. Chemical fractions refused due to the presence of the aversive substance sensed by the sheep were fractionated until a purified substance had been isolated. Nuclear magnetic resonance (NMR) analysis of the purified substance showed it to be sceleratine nitrogen oxide, the toxic principle of S. latifolius.

Effects Of Short-Term Pesticides Exposure On Swimming Behavior, Tissue Morphology, Oxidative-Nitrative Stress, Antioxidant Expression, And Cellular Apoptosis In Kidneys Of Goldfish, Esmirna Cantú

Theses and Dissertations

The prevalence of pesticides in the aquatic environment continues to increase due to anthropogenic activities and poses a threat to aquatic organisms. The aim of this study was to determine the short-term exposure (one-week) of pesticide mixtures (metolachlor, linuron, isoproturon, tebucanazole, alconifen, atrazine, pendimethalin; azinphos-methyl) on swimming behaviors, kidney morphology, and expression of nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), superoxide dismutase (SOD), catalase (CAT), renin, and cellular apoptosis in goldfish (Carassius auratus). Results indicated that both low- and high-dose pesticide mixtures decreased the distance swimming behavior. Histological analysis showed pesticide exposure alters kidney morphology. DNP, NTP, and renin expressions, and …

Effect Of Authentic Kefir And Nigella Sativa On Broilers Challenged By Coccidia And Clostridium Perfringens, Julian E. Nixon

All Dissertations

Black seed oil concentrations of 0%, 0.1%, 1% and 5% were added to milk inoculated with kefir grains and incubated at 25°C for 22 h. The pH and microbial count indicated 1% black seed oil caused low inhibition (P > 0.05) of fermentation, but 5% black seed oil caused significant inhibition of the kefir microorganisms (P < 0.05).

Cobb 500 male chicks (n = 256) were distributed in a randomized block design and received one of four treatments: CTRL1 (Non-medicated, no kefir, no Clostridium perfringens), CTRL2 (Non-medicated, no kefir, C. perfringens inoculated), CTRL3 (BMD medicated, no kefir, C. perfringens inoculated), KTRT (Non-medicated, …

Linking The Metabolic And Ecological Fate Of Caribbean Ciguatoxins And Related Neurotoxins In Reef Herbivores, Jessica K. Gwinn

Graduate Theses and Dissertations (2019 - present)

Ciguatera poisoning (CP) is one of the most prominent seafood-borne illnesses worldwide, affecting between 25,000–500,000 people annually. Ciguatera is caused by the ingestion of seafood contaminated with algal-derived ciguatoxins (CTX), the precursors of which are produced by benthic and toxigenic dinoflagellates in the genera Gambierdiscus and Fukuyoa. These compounds enter marine food webs through herbivory and are subsequently transferred to higher trophic levels, undergoing complex metabolic biotransformations, which produce structurally related CTX derivatives. Consumption of CTX induces a variety of severe neurological, gastrointestinal, and/or cardiovascular symptoms beginning within hours and lasting for weeks to months following exposure. Despite the impact …

Cats Of Unl: An Investigation Into The Human-Feral Cat Relationships On A University Campus In Nebraska, Jacey Skoda

Honors Program: Senior Projects (Public)

At the University of Nebraska-Lincoln (UNL), there exists a population of feral cats that is monitored and cared for by a group known as Husker Cats. As the cats wander the sidewalks and gardens of the university, they may appear non-threatening and pleasant to have around. However, a feral cat presence could have detrimental impacts on the surrounding ecosystem, property, and community. The first component of this project, a survey of individuals on UNL campus, ultimately seeks to understand what those in the UNL community know about the feral cats, how they feel about the feral cats, and how they …

Pharmacokinetics Of Single Feeding Of Cannabidiol In Cattle: A Pilot Study, Haley Cornette

Honors College Theses

Cannabidiol (CBD) is a substance that has been used in complementary medicine for many years. However, modern medicine has little knowledge of how this substance is utilized and metabolized in ruminant animals. Regulations on quality assurance and use in animals are lacking, and CBD supplementation in livestock is not approved. If CBD supplements can be shown to be safe and effective, detection in the animal will be important for determining regulation of use. A withdrawal period can then be established to allow time for deterioration of product to safe levels before livestock products enter human markets. This study sought to …

Telomeres: A Tool To Assess The Impacts Of Mining Contaminants On Riparian Songbirds, Lillian Krach, Bridger Creel, Megan Fylling, Zac Cheviron, Creagh Breuner

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Mining has left massive environmental and physical scars across the landscape. Aquatic and riparian landscapes in particular have been significantly impacted by traditional mining practices. Waste products left over from hard-rock mining leech heavy metals onto the landscape and these metals spread from headwater streams to major waterways (Lottermoser 2010). Heavy metals have been shown to cause physiological stress and challenges to organisms depending on the metal and the concentration (Baos et al. 2019, Boyd & Rajakaruna 2013). While some mining-impaired areas have undergone restoration efforts, is it enough? Typical restoration methods replace the contaminated floodplain, but not the riverbed …

Chemical Immobilization Of Helicopter-Captured Elk (Cervus Canadensis) And Survival Of Elk Calves In Southeastern Kentucky, Kathleen E. Williams

Theses and Dissertations--Forestry and Natural Resources

Safe and effective chemical immobilization is critical to minimize stress and risk of injury when capturing free-ranging, wild ungulates. Many traditionally favored high potency opioids have been phased out or become unavailable because of increased regulations, leading to the development of two pre-mixed combination drugs, butorphanol-azaperone-medetomidine (BAM) and nalbuphine-medetomidine-azaperone (NalMed-A). Both drugs have been used to chemically immobilize ungulates, but their efficacy has not been documented in elk captured and transported via helicopter. During 2020 – 2022, we chemically immobilized helicopter-captured female elk (Cervus canadensis) with a single IM-injection of BAM (n = 41) or NalMed-A (n = …

Bioaccumulation Of Polychlorinated Biphenyl Compounds And Mercury In A Mining Impacted Aquatic Ecosystem, Michelle Bollini

Dissertations, Master's Theses and Master's Reports

The Keweenaw area continues to be influenced by the century of copper mining that ended nearly 50 years ago. This project is focused on Torch Lake, an aquatic ecosystem that has been heavily impacted by mining waste disposal. The watershed has been impaired by mine discharge and tailings, smelter and smokestack plumes, and poor waste disposal practices. The lake is listed as a Great Lakes Area of Concern with beneficial use impairments of restrictions on fish consumption and a degraded benthic community. Polychlorinated biphenyl compounds (PCBs) and methylmercury (MeHg) are persistent, bioaccumulative, and toxic substances (PBTs). These contaminants pose threats …

Assessment Of Spilled Toxic Bait By Wild Pigs And Potential Risk To Non-Target Species, John C. Kinsey, Justin A. Foster, Nathan P. Snow, Jason D. Wishart, Linton D. Staples, Janis K. Bush, Kurt V. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Background

In 2018, a sodium nitrite (SN)-based toxic bait for invasive wild pigs (hereafter wild pigs; Sus scrofa), was evaluated to determine its effectiveness in reducing local wild pig populations in Texas. Localized population reductions of > 70% were achieved, but spillage of bait outside wild pig-specific feeders (bait stations) caused by feeding wild pigs resulted in the deaths of non-target animals. To evaluate risks to non-target animals, we tested whether bait presentation influenced the total amount of bait spilled by wild pigs and estimated the associated risk to non-target species.

Results

We found that bait spilled outside bait stations …

Differences In Drifting Invertebrate Communities Across Arctic Ecozones And The Influence On Potential Growth Of Grayling (Thymallus Arcticus), Charles Chanyi

Theses and Dissertations (Comprehensive)

Invertebrate drift is a key process that potentially affects multiple levels of food web organization within stream environments. However, our understanding of the mechanistic drivers of drift in high latitude streams and subsequent bottom-up control that drift may have on fish predators in these environments remains understudied. This project aimed to gain the baseline knowledge of how drift functions across two major high latitude ecozones, the boreal forest and tundra, and how those possible differences in drifting community characteristics may impact drift-feeding Arctic grayling (Thymallus arcticus). These objectives were accomplished by characterizing stream environments across both ecozones, sampling …

The Path To U.S. National Registration Of A Toxic Bait For The Control Of The Small Indian Mongoose, Carmen C. Antaky, Steven C. Hess, Emily W. Ruell, Israel L. Leinbach, Shane R. Siers, Robert T. Sugihara

Human–Wildlife Interactions

The small Indian mongoose (Urva auropunctata [syn. Herpestes auropunctatus]; mongoose) is a highly invasive species in its introduced range that negatively impacts ecosystems. Mongooses depredate native species, serve as a vector of disease posing a risk to human health, and cause sanitation issues in food processing facilities and public areas. Introduced for biocontrol in the late 1800s in Hawaiʻi and the Caribbean, mongooses currently have well-established populations across multiple islands in both island archipelagos and have invaded numerous other locations throughout the world. The concern of accidental introduction to mongoose-free islands, the difficulty in species detection, and the …

Inferring Exposure To Harmful Pseudo-Nitzschia Blooms From Ocean-To-Estuary Gradients In Domoic Acid Concentrations In Humboldt Bay Bivalves, Natasha Hope Ficzycz Winnacott

Cal Poly Humboldt theses and projects

Harmful algal blooms (HABs) result from outbreaks of any of several different species of toxin-producing phytoplankton and that can have major detrimental effects on marine ecosystems and pose severe health and economic threats to human communities. Of particular concern along the United States West Coast are HABs of pennate diatom genus Pseudo-nitzschia that produce the potent neurotoxin domoic acid (DA). The coastal ocean between Cape Mendocino, CA, and Cape Blanco, OR is a hotspot for Pseudo-nitzschia spp. HABs. Such blooms impact coastal fisheries and pose a potential threat to aquaculture operations in Humboldt Bay, California’s second largest estuary and largest …

Detection And Observed Correlations Of Statin And Beta-Blocking Pharmaceuticals In West Virginia Surface Waters And Ichthyofauna, Joseph W. Kingsbury

Graduate Theses, Dissertations, and Problem Reports (ETD)

Within this thesis the source, presence, and the estimated ecotoxicological effects of cardiovascular medicines, specifically statins and beta-blockers are described within the Buckhannon, Tygart Valley, and West Fork Rivers of West Virginia. Included within this thesis is a general introduction providing key background information and relevant research related to the source, presence, and known toxicity of statins and beta-blockers. Following the general introduction there are 3 distinct chapters. Chapter 1 focuses on the estimated demographic based source loadings and the surface water concentration characteristics of statins and beta-blockers within the three rivers. Chapter 2 investigates the effects of statins and …

Effects Of Microcystin-Lr On Channel Catfish (Ictalurus Punctatus) Susceptibility To Microbial Pathogens (Aeromonas Hydrophila And Edwardsiella Piscicida), Alison Marchant

Theses and Dissertations

Microcystin-LR is a hepatotoxin produced by cyanobacteria. Aeromonas hydrophila and Edwardsiella piscicida infections are leading causes of losses in market-sized channel catfish (Ictalurus punctatus). These older fish should have natural immunity in place and a predisposing factor is likely a prerequisite for these disease outbreaks. While microcystin-LR rarely causes mortality in warm-water aquaculture, we believe it may be a predisposing factor that leads to bacterial disease outbreaks during the summer months due to its ability to damage the liver. Our study investigated microcystin-LR’s effects on channel catfish susceptibility to these pathogens. We found that a sublethal dose of microcystin-LR induced …

Effects Of Titanium Dioxide Nanoparticle Injections On Tissue Function And Morphology In Goldfish (Carassius Auratus), Bryan Joel Patterson

Graduate Theses

Fish gills are sensitive to a variety of environmental factors because they regulate the exchange of oxygen and ions between the fish and the external environment. This study examined the effects of polyacrylic acid (PAA) coated titanium dioxide nanoparticle injections (TiO₂ NPs) on goldfish (Carassius auratus) over 14 days. Following the experimental period, we examined gill histological samples and looked for signs of an inflammatory response through blood glucose levels and relevant gene expression. TiO₂ injected fish developed a more robust ILCM (interlamellar cell mass) compared to the non-injected controls, which remained elevated over 14 days post injection. …

Critical Corridor: Survey Of Predator Occurrence And Habitat Use In A Threatened Rainforest Protected Area: Comatsa-Sud And Marojejy National Park, Madagascar, Patrick H. Ross

Theses

Madagascar is a nation praised for its floral and faunal endemism and biodiversity. Among the island nation’s most emblematic fauna are its native mammalian carnivores; they are members of the threatened and endemic Eupleridae family. The Corridor of Marojejy – Anjanaharibe Sud – Tsaratanana (COMATSA) is a system of forest protected areas in which three large protected areas are connected in northern Madagascar: Tsaratanana Reserve, Anjanaharibe Sud Special Reserve and Marojejy National Park which is also a UNESCO World Heritage site. COMATSA is a newly protected and currently threatened corridor system that lacks detailed surveys and assessments of its native …

Accumulation Of Persistent Organic Pollutants In Marine Mammals: A Case Study On Cetaceans, Pinnipeds, And Sirenians, Alydia Moorhead

All HCAS Student Capstones, Theses, and Dissertations

Persistent organic pollutants (POPs) are lipophilic semi-volatile organic chemicals that present a range of challenges to marine biota, specifically marine mammals that often occupy a high trophic position in the food web. POPs have become a global problem since they have been shown to cause immunologic, teratogenic, carcinogenic, neurological, and reproductive complications in living organisms due to their resistance to biodegradation and their lipophilic nature. Marine mammals can accumulate these toxic substances through direct ingestion, trophic transfer, adsorption, and maternal offloading. They are susceptible to both bioaccumulation and biomagnification of POPs. Accumulation of POPs is affected by many variables, including …