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Full-Text Articles in Molecular and Cellular Neuroscience
Amino Acid Sensing Ability By The Mechanistic Target Of Rapamycin In Oligodendrocytes, Emma England
Amino Acid Sensing Ability By The Mechanistic Target Of Rapamycin In Oligodendrocytes, Emma England
Senior Theses and Projects
Oligodendrocytes (OLs) are a type of glial cell in the central nervous system that require substantial nutrients such as lipids and amino acids to create the extensive, metabolically expensive myelin sheath surrounding the axons of neurons. The mammalian target of rapamycin (mTOR) is a regulatory kinase that is necessary for the maturation of progenitor OLs through their distinct oligo-lineage phases. Amino acids are vital for the functioning of cells. The focus of this study was to determine if mTOR activity is dependent on the availability of leucine, methionine, and alanine. This was assessed by examining the phosphorylation level of the …
Mtor Signaling And Endoplasmic Reticulum Stress In Mixed Glial Cultures, William Theodore Schreiber-Stainthorp
Mtor Signaling And Endoplasmic Reticulum Stress In Mixed Glial Cultures, William Theodore Schreiber-Stainthorp
Senior Theses and Projects
Oligodendrocytes are the myelinating cells of the central nervous system. The extraordinary protein and lipid synthesis that myelin production entails makes oligodendrocytes especially prone to endoplasmic reticulum (ER) stress. In this experiment, we set out to investigate the dynamics of ER stress in mixed glial cultures, and how the consequences of that stress vary based on the activity of the anabolic PI3K/AKT/mTOR pathway. Previous experiments in isolated oligodendrocyte cultures demonstrated a negative correlation between AKT/mTOR signaling and oligodendrocyte cell viability in the presence of ER stressors. Because ER stress leads to a buildup of improperly processed proteins, decreases in mTOR …