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Full-Text Articles in Virology
Influence Of Bovine Respiratory Syncytial Virus F Glycoprotein N-Linked Glycans On In Vitro Expression And On Antibody Responses In Balb/C Mice, Holly A. Klink, Ryan Brady, Christina L. Topliff, Kent M. Eskridge, Subramaniam Srikumaran, Clayton L. Kelling
Influence Of Bovine Respiratory Syncytial Virus F Glycoprotein N-Linked Glycans On In Vitro Expression And On Antibody Responses In Balb/C Mice, Holly A. Klink, Ryan Brady, Christina L. Topliff, Kent M. Eskridge, Subramaniam Srikumaran, Clayton L. Kelling
Nebraska Center for Virology: Faculty Publications
Bovine respiratory syncytial virus (BRSV) is an etiological component of the bovine respiratory tract disease complex. Infection with BRSV following vaccination, or re-infection following natural infection is common since protection is incomplete. The objectives of this study were to create plasmid DNA constructs encoding single or multiple N-glycosylation-site deletion BRSV fusion (F) proteins, and evaluate their expression in cell culture, and potential to induce anti-BRSV F antibody responses in BALB/ c mice. Four plasmid DNAs were constructed, each encoding 1-4 N-glycosylation-site deletions: Gly4, Gly2/4, Gly1/2/4 and Gly1/2/3/4. Each of the N-glycosylation-site deletion BRSV F proteins were expressed …
In Vitro Expression Of Full-Length And Truncated Bovine Respiratory Syncytial Virus G Proteins And Their Antibody Responses In Balb/C Mice, Ryan P. Brady, Christina L. Topliff, Clayton L. Kelling
In Vitro Expression Of Full-Length And Truncated Bovine Respiratory Syncytial Virus G Proteins And Their Antibody Responses In Balb/C Mice, Ryan P. Brady, Christina L. Topliff, Clayton L. Kelling
Nebraska Center for Virology: Faculty Publications
Bovine respiratory syncytial virus (BRSV) is a primary cause of lower respiratory tract disease in calves. Protection is incomplete following vaccination or natural infection, as re-infections are common. The objectives of this study were to create plasmid DNA constructs encoding the full-length, secreted, or conserved region of the BRSV G glycoprotein, and to compare and evaluate their expression in cell culture and potential to induce antibody responses in BALB/c mice. Transfection of COS-7 cells with plasmid DNA resulted in expression of the BRSV G region from each of the plasmid DNA constructs. Following inoculation of BALB/c mice with plasmid DNA, …