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Organismal Biological Physiology Commons™
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- Antibiotics (2)
- Bicarbonate buffer (2)
- Canine (2)
- Culture system (2)
- Decontamination (2)
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- Electric slicer (2)
- Fluorescence microscope (2)
- Genetic diversity (2)
- Germ cells (2)
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- Live dead cell stain (2)
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- Reproductive biology (2)
- Sperm bank (2)
- Spermatogenesis (2)
- Testes (2)
- Testicular cancer (2)
- Testosterone (2)
- Tissue culture (2)
- Vibratome (2)
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Articles 1 - 3 of 3
Full-Text Articles in Organismal Biological Physiology
Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
2023 Symposium
Canine testes thin section culture
Testes tissue culture systems would provide a tool to elucidate spermatogenesis mechanisms, with the aim of genetic preservation of mammals, especially endangered species. Our experiment aims to develop a culture system capable of producing viable mammalian sperm cells in vitro.
Dogs were chosen as the model organism because testes are readily available. Canine testes were obtained from a local veterinary clinic. Thin sections were generated using a commercial electric slicer. They then were cleaned using Dulbecco’s Phosphate-Buffered Saline (DPBS) supplemented with antibiotics then cultured in a modified Tissue Culture Medium 199 (TCM-199). Sections were …
Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
2023 Symposium
Canine testes thin section culture
Testes tissue culture systems would provide a tool to elucidate spermatogenesis mechanisms, with the aim of genetic preservation of mammals, especially endangered species. Our experiment aims to develop a culture system capable of producing viable mammalian sperm cells in vitro.
Dogs were chosen as the model organism because testes are readily available. Canine testes were obtained from a local veterinary clinic. Thin sections were generated using a commercial electric slicer. They then were cleaned using Dulbecco’s Phosphate-Buffered Saline (DPBS) supplemented with antibiotics then cultured in a modified Tissue Culture Medium 199 (TCM-199). Sections were …
The Bi-Directional Relationship Between Gut Microbiota And Autoimmunity, Trevor O. Kirby
The Bi-Directional Relationship Between Gut Microbiota And Autoimmunity, Trevor O. Kirby
EWU Masters Thesis Collection
Humans serve as a major reservoir for a vast number of microbiota. These microbes have evolved symbiotic relationships with humans due to their close proximity with their host. As a result, the immune system adapts to the microbiota thus modulating immunological function. Autoimmunity is a state in which there are aberrant immune responses produced against host tissue. Intestinal bacteria are directly impacted by instances of inflammation brought on by autoimmunity. The complicated nature between autoimmunity and bacterial modulation demonstrates a bi-directional relationship. Here, we utilize experimental autoimmune encephalomyelitis, a model for multiple sclerosis, to explore the bi-directional relationship that disease …