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Articles 91 - 92 of 92
Full-Text Articles in Bacteriology
Selection And Characterization Of Cole1 Plasmid Mutants That Exhibit Altered Stability And Replication., Joseph Inselburg
Selection And Characterization Of Cole1 Plasmid Mutants That Exhibit Altered Stability And Replication., Joseph Inselburg
Dartmouth Scholarship
This report describes a method for isolating mutants of plasmid ColE1 that exhibit unstable maintenance and altered replication characteristics. It also describes the initial characterization of four mutants isolated by that method. A chimeric plasmid, pHSG124, containing a ColE1 derivative and a temperature-sensitive replication derivative of pSC101 was mutagenized in vitro, using hydroxylamine. By adjusting the growth conditions of transformants containing the mutagenized chimeric deoxyribonucleic acid, it was possible to rapidly screen colonies and identify those that had a high probability of carrying ColE1 mutants that exhibit unstable maintenance. Of those mutants, some exhibited altered copy number or accumulated catenated …
Functional Relationship Between Parts Of The Replication Region Of Plasmid Cole1., Joseph Inselburg
Functional Relationship Between Parts Of The Replication Region Of Plasmid Cole1., Joseph Inselburg
Dartmouth Scholarship
The inhibition of plasmid ColE1 replication caused by a deletion of the ColE1 plasmid replication origin has been previously reported (T. Hashimoto-Gotoh and J. Inselburg, J. Bacteriol. 139:597-619). Evidence is presented showing that restoration of the deleted nucleotide sequence in the precise relationship it normally has to the rest of the replication region is essential for restoration of ColE1 replication capability to the deletion mutant.