Bacteriology Commons^™

Lumpy Wool May Increase By Dipping : Research Roundup, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Lumpy wool, or mycotic dermatitis, is an infectious disease on the skin of the sheep caused by the bacterium Dermatophilus conglensis. The organism invades the wool and hair follicles, causing formation of skin lesions producing scabby material which binds the wool fibres into a hard mass.

Research shows that 'dipping' may increase the incidence of lumpy wool with some chemicals a greater risk than others.

The Role Of D-Gluconic Acid In The Regulation Of The Synthesis Of The Enzymes Of The Entner-Doudoroff Pathway In Pseudomonas Fluorescens, Steven C. Quay

Honors Theses

Induction of Entner-Duodoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but not glucose, induced synthesis of gluconokinase and 6-phosphogluconate dehydratase in both mutants. High constitute levels of 2-keto-3-deoxy-6-phosphogluconate aldolase were found when both mutants were grown on glucose. Growth of parent and both mutant strains on glycerol also resulted in high levels of Entner-Doudoroff pathway enzymes. It was concluded …

Bacteriuria As A Precursor Of Intracatheter Encrustation, Namcy Moll

Loma Linda University Electronic Theses, Dissertations & Projects

The problem with which this study was concerned was the development of criteria that could be used to determine when urinary catheter changes were necessary. By determining when encrustation occurred, it was felt that some complications could be eliminated by changing catheters before obstruction occurred due to encrustation.

It was the purpose of this study to: 1) explore some indications for catheter change, 2) determine if encrustation of urinary catheters necessitates catheter change, and 3) estimate potential criteria for a routine time for catheter changes.

Forty-five catheters were examined after their removal from patients. Flow rates were determined on 33 …

The Effect Of Elevated Glucose On Neutrophilic Phagocytosis In Genetically Diabetic Mice, Larry B. Thompson

Loma Linda University Electronic Theses, Dissertations & Projects

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each …

The Effectiveness Of Camphorated Paramonochlorophenol, Metacresyl Acetate, And Prednisolone In Combination As An Antimicrobial Agent, Maurice Cutler

Loma Linda University Electronic Theses, Dissertations & Projects

Post-treatment discomfort following endodontic procedures can be caused by inflammation due to injudicious use of intracanal medicaments. Camphorated paramonochlorophenol, metacresyl acetate, and prednisolone in combination is advocated to reduce the inflammatory potential of the germicide and thus reduce the occurrence of post-operative pain. It is imperative that a medicament retain antimicrobial activity while reducing inflammatory potential.

This investigation was undertaken toward determining on an in vitro quantitative bactericidal versus bacteriostatic basis by use of the broth dilution test tube method whether camphorated paramonochlorophenol, metacresyl acetate, and prednisolone in combination retains antimicrobial activity comparable to selected other commonly used nonspecific germicides. …

Can Mastitis Be Eliminated?, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Can mastitis be eliminated from a dairy herd? The results of a 12-month continuous study of four commercial herds show that it could be done.

The Effect Of Glucose On Neutrophilic Phagocytosis, Donald F. Adams

Loma Linda University Electronic Theses, Dissertations & Projects

Samples of blood were obtained from thirty healthy young adult males (Loma Linda University School of Dentistry), and contaminated with bacteria. Varying concentrations of pure glucose were added to this mixture to observe the effect of the glucose on in vitro neutrophilic phagocytosis. As the glucose levels increased a statistically significant suppression was noted in the ability of the neutrophilic leukocytes to engulf Staphylococcus epidermidis.

It was not possible, however, to predict the degree of suppression for any particular level of glucose added nor is it possible to state that suppression will occur in any one individual.

Two methods of …

Biochemical Properties Of Salmonella Potsdam Bacteriophage P4/2, Clyde L. Roggenkamp

Loma Linda University Electronic Theses, Dissertations & Projects

Over 90% recovery of phage P4/2 was realized with differential centrifugation concentration and purification, whereas only about 16% was recovered from the aqueous two-phase polymer - Genetron - ECTEOLA-cellulose column system. Purity of preparations was greatest with the density gradient centrifugation (A₂₆₀ = 2.66 and A₂₈₀ = 1.80 per 10¹² plaque-forming units). With the chemical system of concentration and purification followed by an ECTEOLA anion exchange run, the A₂₆₀ was 6.6 and the A₂₈₀ was 4.3.

Three tests were employed to determine the configuration of P4/2 DNA: (1) a formalin denaturation test, (2) a thermal …

The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay

Loma Linda University Electronic Theses, Dissertations & Projects

A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.

Conventional mice were challenged intraperitoneally with a single near LD₅₀ dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …

Absence Of Pasteurella Pestis In The Wildlife Of Arkansas, R. Reece Corey, Leo J. Paulissen, Delbert Swartz

Journal of the Arkansas Academy of Science

No abstract provided.

A Study Of The Bacteriological Effectiveness Of Oral Thermometer Procedures In Three Selected Hospitals, Esther Mae Rose

Loma Linda University Electronic Theses, Dissertations & Projects

Because pathogens such as streptococcus and staphylococcus have been transferred in many hospitals, the source of the infections is of concern to doctors, nurses, and other medical personnel. As shown by the review of literature, good techniques are necessary to stop the spread of disease.

It was the purpose of this study to investigate one facet of patient safety by: (1) the culture of thermometers ready for use in three selected hospitals; (2) to determine the effectiveness of the Group, Individual, and Central Supply methods of disinfection currently practiced in the three selected hospitals.

The method for the experiment of …