Microbiology Commons^™

The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay

Loma Linda University Electronic Theses, Dissertations & Projects

A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.

Conventional mice were challenged intraperitoneally with a single near LD₅₀ dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …

Molecular Taxonomy Of Triatominae, Henry D. Brodie

Loma Linda University Electronic Theses, Dissertations & Projects

A taxonomic study of the subfamily Triatominae was conducted to determine systematic relationships using agar gel diffusion methods in the differentiation of ova antigens and electrophoresis to differentiate hernolymph proteins.

Agar gel diffusion methods indicated that two distinctive Triatominae species from South America could be distinguished from typical North American species; it was also possible to distinguish the monotypic genus Dipetalogaster from other North American populations.

A total of 180 electrophoretic tracings of Triatominae hernolyrnph were made from 18 populations of kissing bugs. Closely related populations of the Triatoma Protracta, Triatoma Rubida, and Paratriatoma Hirsuta Complexes showed group relationships within …

The Ultraviolet Induction Properties Of Artificially Lysogenized Strains Of Salmonella Potsdam, Roy E. Olson

Loma Linda University Electronic Theses, Dissertations & Projects

By infection of the non-lysogenic strain of S. potsdam, SP2, with the phages P9a/2 and P9/c three artificially lysogenized strains were isolated: SP2-9a, SP2-9c, and SP2-9ca. SP2 lysogenized with P9a could not further be lysogenized with P9c.

There was evidence from the induction data that the presence of P9c influenced the induction of phage P9a, although P9a did not appear to influence the induction of P9c. Much greater levels of free phage P9a were achieved in induced cultures of SP2-9ca than in induced cultures of SP2-9a.

There were marked differences in induction of both phages P9a and P9c between …

Nocturnal Flights Of Triatoma Protracta (Uhler) As Indicated By Collections At Black Light Traps : (Hemiptera: Reduviidae: Triatominae), Robert D. Sjogren

Loma Linda University Electronic Theses, Dissertations & Projects

An investigation into the environmental and ethological factors relating to summer flights of Triatoma protracta protracta (Uhler) was performed utilizing black light traps of original design. Field observations were conducted on 51 evenings during the period June 1 to October 31, 1964. The traps were operated on a surveillance basis for the remainder of a twelve month period.

A total of 398 adult Triatoma protracta were collected in traps around a residence located in the chaparral covered foothills 10 miles east of Redlands, California. In addition to observations on field behavior, laboratory dissections were performed on each bug to obtain …

Ultraviolet Induction Properties Of Salmonella Potsdam Bacteriophages, Berney Roy Neufeld

Loma Linda University Electronic Theses, Dissertations & Projects

Three lysogenic strains of Salmonella potsdam were studied to determine whether or not they were inducible by ultraviolet light under certain conditions. The strains examined were SP3, SP4, and SP9. SP3 and SP4 contained a single prophage each and SP9 contained two prophages P9a and P9c. The ultraviolet light sensitivities of each of these lysogenic strains and the indicator strain SP2 were also examined.

All three lysogenic strains were found to be inducible. SP3 was induced an average of 3.59% and SP4 an average of 2.21%. The difference between the induction percentages of SP3 and SP4 was not statistically significant. …

A Study Of Door Pulls On Isolation Carts In A General Hospital For Evidence Of Contamination, Mary Alice Harper

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of the study was to find out if isolation carts were serving as a means of cross infection. Literature pertaining to sources of possible contamination on hospital units written in the past five years were reviewed.

Using the descriptive survey as the method of study, effort was made to find out if isolation carts were a means of cross infection on the hospital unit. Two laboratory cultures were obtained from both door pulls on all isolation carts used by nursing units. This was done over a three month period from December 12, 1963 to March 11, 1964. The …

Some Physical And Chemical Properties Of Salmonella Potsdam Bacteriophages, Norma W. Lee

Loma Linda University Electronic Theses, Dissertations & Projects

The phages isolated from different lysogenic strains of Salmonella may be divided into different groups on the basis of their heat resistance. In Group A are phages resistant to a temperature of 80°C and in Group B phages that are killed at this temperature. Three temperate bacteriophages P3/2, P4/2 and P9a/2 isolated from lysogenic strains of S. potsdam were investigated. Phage P4/2 is in Group A and phages P3/2 and P9a/2 in Group B.

The kinetics of heat inactivation and of ultraviolet light inactivation of each of these phages were compared. Base ratio analysis of the nucleic acid by the …

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

Life History Of A Haemogregarine, Hepatozoon Sp. In The Chuckwalla: Sauromalus, John E. Lewis

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Evaluation Of The Photometric Assay Method To Quantitate The Bactericidal Action Of Normal And Immune Rabbit Sera On Salmonella Pullorum, Alice Margaret Rahn

Loma Linda University Electronic Theses, Dissertations & Projects

The bactericidal antibody test depends on the presence of sufficient complement, magnesium ions, and specific antibody to produce killing of certain gram negative bacilli. This study was undertaken to demonstrate whether or not Salmonella pullorum is an organism which is susceptible to this killing action.

Different amounts of test serum containing antibody, constant amounts of complement and magnesium saline diluent were placed together and held in a 37°C water bath for a one hour reaction time. During this interval, the antibody-complement system was given an opportunity to kill the susceptible bacteria. Then, a constant volume of nutrient broth was added …

The Effect Of Infection With Polyoma Virus On The Protein, Rna, And Dna Of Mouse Embryo Cell Cultures, Lawrence E. Mcconnehey

Loma Linda University Electronic Theses, Dissertations & Projects

In four separate experiments mouse embryo cell monolayers infected with polyoma virus and uninfected control mouse embryo cells were maintained in Eagle’s Minimum Essential Medium for periods of time ranging from 0 to 168 hours. The cell layers were removed from the glass by trypsinization. Samples of the cells were counted in a Coulter Counter and the remainder of the cells frozen after centrifugation, in a button. Later, the buttons were thawed and protein, RNA, and DNA analyses were performed on each sample. The micrograms per million cells of protein. RNA, and DNA were determined for each sample and plotted …

Quantitative Evaluation Of Streptococcal Antibodies By A Serum Inhibition Fluorescent Antibody Tube Method, Charles Glenn Nichols

Loma Linda University Electronic Theses, Dissertations & Projects

The development and description of a quantitative method of evaluating streptococcal antibodies by inhibition of fluorescent staining is reported. Suspensions of Streptococcus pyogenes group A, treated with methyl alcohol to reduce auto-fluorescence, were used as antigen. The degree of inhibition of uptake of fluorescent labeled antibody produced by the presence of antibodies in the immune test sera was measured after elution of the labeled antibody. Comparisons were made with controls using normal sera containing no inhibiting antibodies. A method of reporting the results in terms of titer is described.

Streptococcal antibodies were demonstrated quantitatively by this inhibition test in sera …

A Comparative Study Of The Growth Of Polyoma Virus In Two Secondary Cell Lines: Mouse Lymphoma And Normal Mouse Fibroblasts, Rheeta M. Stecker

Loma Linda University Electronic Theses, Dissertations & Projects

In comparing the growth of polyoma virus in secondary cell cultures of a mouse lymphoma (P388D₁, a known polyoma-supporting strain) and normal mouse fibroblasts (Earle’s L cells), the following observations have been made.

The L cell is capable of supporting the growth of the polyoma virus with reproduction and release of infectious particles. Viruses appear in the fluid from time to time over a period of several weeks. This probably represents a carrier state of the virus in L cells. Many of the L cells in infected cultures are not killed by the virus infection.

Virus particles released …

A Study Of The Bacteriological Effectiveness Of Oral Thermometer Procedures In Three Selected Hospitals, Esther Mae Rose

Loma Linda University Electronic Theses, Dissertations & Projects

Because pathogens such as streptococcus and staphylococcus have been transferred in many hospitals, the source of the infections is of concern to doctors, nurses, and other medical personnel. As shown by the review of literature, good techniques are necessary to stop the spread of disease.

It was the purpose of this study to investigate one facet of patient safety by: (1) the culture of thermometers ready for use in three selected hospitals; (2) to determine the effectiveness of the Group, Individual, and Central Supply methods of disinfection currently practiced in the three selected hospitals.

The method for the experiment of …

Natural History Of Thelazia Californiensis Price, 1930 (Nematoda: Thelaziidae), A Mammalian Eye Worm, Warren E. Parmelee

Loma Linda University Electronic Theses, Dissertations & Projects

Various factors—embryology, host associations, and seasonal changes In eye worn populations—tending to promote the propagation of T. califomlensls were studied.

The early embryology closely parallels that of other nematode species.

The mature embryos remain alive in saline at 35°C as long as 5 days. Dead embryos can be distinguished from living ones by virtue of the rapid staining by methylene blue by the former. Parturient female thelazias release embryos into saline, and embryos are found regularly in tear secretions of infected laboratory rabbits.

Ovic embryos possess an enveloping egg membrane containing chitin. On this account, this eye worm species is …

Natural And Experimental Infections Of Thelazia Californiensis Price (1930, Nematoda: Thelaziidae) With Descriptions Of The Adult And Larval Parasites, Howard S. Burnett

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this work was to determine the extent and effects of infestation of the eye worm of mammals, Thelazia californiensis, and to elucidate through experimentation or observation the mode of transmission.

A survey of literature revealed that thelaziasie (infestation by Thelazia species) may cause serious pathological changes in the eye and its adnexa, sometimes resulting in blindness. It was found that reports of thelaziasis in the United States were (with two exceptions) of cases in California. It was further found that T. californiensis has been reported in a wider variety of host than has any of other …

Avian Erythrocyte Receptor Substance For The Natural Hemolysin In Rat Serum, Richard Vincent Johnson

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract not included]

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