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Articles 811 - 840 of 875

Full-Text Articles in Microbiology

Amino Sugars In The Glycoprotein Toxin From Bacillus Thuringiensis Subsp. Israelensis, Mary Ann Pfannenstiel, Ganapathy Muthukumar, Graham A. Couche, Kenneth W. Nickerson Feb 1987

Amino Sugars In The Glycoprotein Toxin From Bacillus Thuringiensis Subsp. Israelensis, Mary Ann Pfannenstiel, Ganapathy Muthukumar, Graham A. Couche, Kenneth W. Nickerson

Papers in Microbiology

The carbohydrate content of purified Bacillus thuringiensis subsp. israelensis crystal toxin was determined by six biochemical tests, column chromatography on an amino acid analyzer, and the binding of 11 fluorescent lectins. The crystals contained approximately 1.0% neutral sugars and 1.7% amino sugars. The anmino sugars consisted of 70% glucosamine and 30% galactosamine. No N-acetylneuraminic acid (sialic acid) was detected. The presence of amino sugars was confirmed by the strong binding of fluorescent wheat germ agglutinin and the weak binding of fluorescent soybean agglutinin. These lectins recognize N-acetyl-D-glucosamine and N-acetyl-D-galactosamine, respectively. The lectin-binding sites appeared evenly distributed among …

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Nucleotdie Sequence Of An Anti-Fluorescyl Hapten Antibody Heavy Chain Variable Region Gene From A Balb/C Mouse Hybridoma Cell Line, Zhen-Qian Liu, Charles Wood, Tai Te Wu Jan 1987

Nucleotdie Sequence Of An Anti-Fluorescyl Hapten Antibody Heavy Chain Variable Region Gene From A Balb/C Mouse Hybridoma Cell Line, Zhen-Qian Liu, Charles Wood, Tai Te Wu

Nebraska Center for Virology: Faculty Publications

We report here the nucleotide sequence of an anti-fluorescyl hapten antibody heavy chain variable region gene from a BALB/c mouse hybridoma cell line 3-13 (1). This gene segment was contained in a 5.0 kb EcoRI fragment isolated by a 700 bp probe 3' to the mouse heavy chain joining minigene region between the XbaI and EcoRI sites. Mature protein starts at *. The first 21 amino acid residues have also been confirmed by direct amino acid sequencing and they are identical to those of immunoglobulin MOPC104E heavy chain (2). Complementarity determining regions (CDRs) are underlined. The third CDR is unusually …

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Productive, Persistent Infection Of Human Colorectal Cell Lines With Human Immunodeficiency Virus, Akio Adachi, Scott Koenig, Howard Gendelman, Daryl Daugherty, Sebastiano Gattoni-Celli, Anthony Fauci, Malcom A. Martin Jan 1987

Productive, Persistent Infection Of Human Colorectal Cell Lines With Human Immunodeficiency Virus, Akio Adachi, Scott Koenig, Howard Gendelman, Daryl Daugherty, Sebastiano Gattoni-Celli, Anthony Fauci, Malcom A. Martin

Nebraska Center for Virology: Faculty Publications

Thirteen adherent human non-lymphocyte cell lines were tested for their susceptibility to infection by human immunodeficiency virus. Productive infection could be demonstrated in three of five colorectal carcinoma cell lines examined; the other eight human non-lymphocyte cell lines were uninfectible. A susceptible colon carcinoma cell line (HT29), as well as normal colonic mucosa, was shown to contain a 3.0-kilobase species of poly(A)+ CD4 RNA, whereas uninfectible colon carcinoma and rhabdomyosarcoma cell lines synthesized no detectable T4 RNA. A persistently infected colon carcinoma cell line was established that continued to produce progeny human immunodeficiency virus for more than 10 weeks …

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Immunological Relationships Among Proteins Making Up The Bacillus Thuringiensis Subsp. Israelensis Crystalline Toxin, Mary Ann Pfannenstiel, Graham A. Couche, Elise J. Ross, Kenneth W. Nickerson Oct 1986

Immunological Relationships Among Proteins Making Up The Bacillus Thuringiensis Subsp. Israelensis Crystalline Toxin, Mary Ann Pfannenstiel, Graham A. Couche, Elise J. Ross, Kenneth W. Nickerson

Papers in Microbiology

The immunological relationships among the proteins of the mosquito larvicidal toxin produced by Bacillus thuringiensis subsp. israelensis have been investigated by using polyclonal antisera specific for the 28-, 70-, and 135-kilodalton proteins. Each of these proteins was immunologically distinct. There was no cross-reaction among the three proteins and the two non-homologous antisera. Treatment of toxin proteins with larval gut enzymes for 20 h identified protease-resistant domains at approximately 65, 38, and 22 kilodaltons. Similar domains were generated by treatment with trypsin and chymotrypsin. Our immunological and kinetic data indicate that the 28-kilodalton protein is degraded successively to protein bands at …

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Analysis Of Mosquito Larvicidal Potential Exhibited By Vegetative Cells Of Bacillus Thuringiensis Subsp. Israelensis, Corey J. Walther, Graham A. Couche, Mary Ann Pfannenstiel, Sara E. Egan, Lisa A. Bivin, Kenneth W. Nickerson Oct 1986

Analysis Of Mosquito Larvicidal Potential Exhibited By Vegetative Cells Of Bacillus Thuringiensis Subsp. Israelensis, Corey J. Walther, Graham A. Couche, Mary Ann Pfannenstiel, Sara E. Egan, Lisa A. Bivin, Kenneth W. Nickerson

Papers in Microbiology

Vegetative Bacillus thuringiensis subsp. israelensis cells (6 x 105/ml) achieved 100% mortality of Aedes aegypti larvae within 24 h. This larvicidal potential was localized within the cells; the cell-free supernatants did not kill mosquito larvae. However, they did contain a heat-labile hemolysin which was immunologically distinct from the general cytolytic (hemolytic) factor released during solubilization of B. thuringiensis subsp. israelensis crystals. The larvicidal potential of the vegetative cells was not due to poly-3-hydroxybutyrate. Instead, it correlated with the ability of vegetative cells to sporulate during the bioassays. No toxicity was observed when bioassays were conducted in the presence …

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Production Of Acquired Immunodeficiency Syndrome-Associated Retrovirus In Human And Nonhuman Cells Transfected With An Infectious Molecular Clone, Akio Adachi, Howard E. Gendelman, Scott Koenig, Thomas Folks, Ronald Willey, Arnold Rabson, Malcom A. Martin Aug 1986

Production Of Acquired Immunodeficiency Syndrome-Associated Retrovirus In Human And Nonhuman Cells Transfected With An Infectious Molecular Clone, Akio Adachi, Howard E. Gendelman, Scott Koenig, Thomas Folks, Ronald Willey, Arnold Rabson, Malcom A. Martin

Nebraska Center for Virology: Faculty Publications

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

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Tropism Of Sheep Lentiviruses For Monocytes: Susceptibility To Infection And Virus Gene Expression Increase During Maturation Of Monocytes To Macrophages, Howard E. Gendelman, Opendra Narayan, Suzanne Kennedy-Stoskopf, Peter G. E. Kennedy, Zahra Ghotbi, J. E. Clements, Jeff Stanley, Gholam Pezeshkpour Apr 1986

Tropism Of Sheep Lentiviruses For Monocytes: Susceptibility To Infection And Virus Gene Expression Increase During Maturation Of Monocytes To Macrophages, Howard E. Gendelman, Opendra Narayan, Suzanne Kennedy-Stoskopf, Peter G. E. Kennedy, Zahra Ghotbi, J. E. Clements, Jeff Stanley, Gholam Pezeshkpour

Nebraska Center for Virology: Faculty Publications

Visna lentiviruses have a natural tropism for cells of the macrophage lineage of sheep and goats, but virus replication in these cells in vivo is restricted so that only small quantities of virus are produced. One restricting factor suggested in previous studies is that virus replication is dependent on the maturity of the cells: the more mature the cell, the less restrictive the replication of the virus. Since monocytes in peripheral blood are precursors of macrophages, we investigated the effect of cell maturation on virus replication under limited control conditions in vitro by inoculating blood leukocytes with virus and retarding …

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Htlv-Iii Serology In Hemophilia: Relationship With Immunologic Abnormalities, Joan C. Gill, Jay E. Menitove, Philip R. Anderson, James T. Casper, Sushilkumar G. Devare, Charles Wood, Stuart Adair, James Casey, Christi Scheffel, Robert R. Montgomery Jan 1986

Htlv-Iii Serology In Hemophilia: Relationship With Immunologic Abnormalities, Joan C. Gill, Jay E. Menitove, Philip R. Anderson, James T. Casper, Sushilkumar G. Devare, Charles Wood, Stuart Adair, James Casey, Christi Scheffel, Robert R. Montgomery

Nebraska Center for Virology: Faculty Publications

We investigated the relationship of the presence of antibodies to HTLV-III and immunologic abnormalities in patients with hemophilia. Serum antibodies to HTLV-III were analyzed by ELISA assay, immunoprecipitation of labeled cell extracts, and immunoprecipitation of purified HTLV-III p24. Thirty-four (61%) of the total group (n = 56) had antibody to HTLV-III; 34 (76%) of 45 patients given commercial factor VIII preparations were seropositive, compared with none of 11 patients treated exclusively with cryoprecipitate obtained from volunteer blood donors. Of patients who were seropositive for HTLV-III antibody, 94% had abnormal T4/T8 ratios, and 33% of those whose serum was antibody …

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Stability Of The Larvicidal Activity Of Bacillus Thuringiensis Subsp. Israelensis: Amino Acid Modification And Denaturants, Mary Ann Pfannenstiel, Graham A. Couche, Ganapathy Muthukumar, Kenneth Nickerson Nov 1985

Stability Of The Larvicidal Activity Of Bacillus Thuringiensis Subsp. Israelensis: Amino Acid Modification And Denaturants, Mary Ann Pfannenstiel, Graham A. Couche, Ganapathy Muthukumar, Kenneth Nickerson

Papers in Microbiology

The Bacillus thuringiensis subsp. israelensis mosquito larvicidal toxin is not a sulfhydryl-activated toxin. The protein disulfide bonds were cleaved and blocked without loss of toxicity. In contrast, modification of the lysine side chains eliminated toxicity. Additionally, the toxin was resistant to high concentrations of salt (8 M NaBr), organic solvents (40% methanol), denaturants (4 M urea), and neutral detergents (10% Triton X-100). However, it was inactivated by both positively and negatively charged detergents and by guanidine hydrochloride.

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Calmodulin Levels In The Yeast And Mycelial Phases Of Ceratocystis Ulmi, Ganapathy Muthukumar, Rajiv K. Kulkarni, Kenneth W. Nickerson Apr 1985

Calmodulin Levels In The Yeast And Mycelial Phases Of Ceratocystis Ulmi, Ganapathy Muthukumar, Rajiv K. Kulkarni, Kenneth W. Nickerson

Papers in Microbiology

The calmodulin content of the yeast and mycelial phases of Ceratocystis ulmi was determined by radioimmunoassay. Calmodulin levels increased at the G1-S boundary of the cell cycle, coinciding with the first visible appearance of buds or germ tubes. However, in both phases the cellular calmodulin levels were equivalent. No differential synthesis was observed.

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Ca(Ii)-Calmodulin Regulation Of Fungal Dimorphism In Ceratocystis Ulmi, Ganapathy Muthukumar, Kenneth W. Nickerson Jul 1984

Ca(Ii)-Calmodulin Regulation Of Fungal Dimorphism In Ceratocystis Ulmi, Ganapathy Muthukumar, Kenneth W. Nickerson

Papers in Microbiology

We have shown that Ca(II) ions, ethylene glycol-bis(β-aminoethyl ether)- N,N-tetraacetic acid, LaCl3, and six known calmodulin inhibitors shift the yeast-mycelium dimorphic potential of Ceratocystis ulmi. Our data are consistent with the conclusions that Ca(II)-calmodulin interaction is necessary for mycelial growth in C. ulmi and that the absence of this interaction leads to the yeast phase.

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Differences In Survivorship, Development Rate And Fertility Between The Long-Winged And Wingless Morphs Of The Waterstrider, Limnoporus Canaliculatus, Anthony J. Zera May 1984

Differences In Survivorship, Development Rate And Fertility Between The Long-Winged And Wingless Morphs Of The Waterstrider, Limnoporus Canaliculatus, Anthony J. Zera

Anthony Zera Publications

Species of waterstriders (Gerridae: Hemiptera) exhibit a remarkable diversity in degree of winglessness (Vepsalainen, 1978; Calabrese, 1980; Zera, 1981). Some species consist exclusively of fully-winged individuals, while other species are composed almost exclusively of wingless individuals; many species exhibit the intermediate case of wing polymorphism and consist of various proportions of fully-winged, short-winged and/or wingless morphs. Wing-polymorphic species often exhibit dramatic spatial and/or temporal variation in morph ratios, both among populations of the same species and among species. Because of this diversity, waterstriders, especially wing polymorphic species, are ideal candidates for the study of the evolution of winglessness (or conversely, …

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Bioassay Of Solubilized Bacillus Thuringiensis Var. Israelensis Crystals By Attachment To Latex Beads, Danny J. Schnell, Mary Ann Pfannenstiel, Kenneth W. Nickerson Mar 1984

Bioassay Of Solubilized Bacillus Thuringiensis Var. Israelensis Crystals By Attachment To Latex Beads, Danny J. Schnell, Mary Ann Pfannenstiel, Kenneth W. Nickerson

Papers in Microbiology

Solubilized crystals of Bacillus thuringiensis var. israelensis were 7,000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8- micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.

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Prevalence Of Extreme Detergent Resistance Among The Enterobacteriaceae, Vance Kramer, Kenneth Nickerson, Nancy V. Hamlett, Caroline O'Hara Feb 1984

Prevalence Of Extreme Detergent Resistance Among The Enterobacteriaceae, Vance Kramer, Kenneth Nickerson, Nancy V. Hamlett, Caroline O'Hara

Kenneth Nickerson Papers

The detergent-resistance properties of 208 independent isolates of the Enterobacteriaceae have been examined. Of these bacterial strains, 200 were able to grow in the presence of ≥5% sodium dodecyl sulfate, including all members of the Klebsielleae tribe. This resistance does not appear to be plasmid encoded. It is proposed that detergent-resistant organisms he termed saponotolerant or saponophilic, by analogy with other microorganisms occupying harsh ecological niches. In contrast to their prevalent resistance to anionic detergents, not one of the 208 strains tested was found to grow in the presence of three different cationic detergents. This sensitivity to cationic detergents may …

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Activation Of A Translocated Human C-Myc Gene By An Enhancer In The Immunoglobulin Heavy-Chain Locus, Adrian C. Hayday, Stephen D. Gillies, Haruo Saito, Charles Wood, Klas Wiman, William S. Hayward, Susumu Tonegawa Jan 1984

Activation Of A Translocated Human C-Myc Gene By An Enhancer In The Immunoglobulin Heavy-Chain Locus, Adrian C. Hayday, Stephen D. Gillies, Haruo Saito, Charles Wood, Klas Wiman, William S. Hayward, Susumu Tonegawa

Nebraska Center for Virology: Faculty Publications

A tissue-specific transcriptional enhancer element that is associated with the human immunoglobulin heavy-chain locus is defined. In a non-Hodgkin’s lymphoma that contains a translocated c-myc gene this enhancer is retained on the 14q+ chromosome and occurs within sequences shown to activate previously cryptic promoters of the c-myc gene.

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Toxicity Of Bacillus Thuringiensis Var. Israelensis Crystals To Aedes Aegypti Larvae: Carbonate Reversal, Danny J. Schnell, Kenneth Nickerson May 1983

Toxicity Of Bacillus Thuringiensis Var. Israelensis Crystals To Aedes Aegypti Larvae: Carbonate Reversal, Danny J. Schnell, Kenneth Nickerson

Papers in Microbiology

The toxicity of purified Bacillus thuringiensis var. israelensis crystals to larvae of Aedes aegypti could be reversed 100-fold by levels of K2CO3 as low as 0.15%.

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Genetic And Environmental Determinants Of Wing Polymorphism In The Waterstrider Limnoporus Canaliculatus, Anthony J. Zera, David J. Innes, Margaret E. Saks Mar 1983

Genetic And Environmental Determinants Of Wing Polymorphism In The Waterstrider Limnoporus Canaliculatus, Anthony J. Zera, David J. Innes, Margaret E. Saks

Anthony Zera Publications

Wing polymorphism commonly occurs in many insects, especially species of Orthoptera, Coleoptera, Homoptera, and Hemiptera (Harrison, 1980). The polymorphism consists of discrete differences in wing length with morphs exhibiting fully developed, reduced, or totally absent wings. In addition to differences in wing length, morphs often differ in a number of other characteristics such as degree of flight muscle development, duration of nymphal development, time to first reproduction, fertility and diapause (Anderson, 1973; Vepsäläinen, 1978; Harrison, 1980).

Wing polymorphism is an attractive system for investigating the evolution of dispersal in natural populations (Vepsäläinen, 1978; Denno and Grissell, 1979; Harrison, 1980). A …

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Two Transcription Products Of The Vesicular Stomatitis Virus Genome May Control L-Cell Protein Synthesis, David D. Dunigan, Jean M. Lucas-Lenard Feb 1983

Two Transcription Products Of The Vesicular Stomatitis Virus Genome May Control L-Cell Protein Synthesis, David D. Dunigan, Jean M. Lucas-Lenard

Nebraska Center for Virology: Faculty Publications

When mouse L-cells are infected with vesicular stomatitis virus, there is a decrease in the rate of protein synthesis ranging from 20 to 85% of that in mock-infected cells. Vesicular stomatitis virus, irradiated with increasing doses of UV light, eventually loses this capacity to inhibit protein synthesis. The UV inactivation curve was biphasic, suggesting that transcription of two regions of the viral genome is necessary for the virus to become inactivated in this capacity. The first transcription product corresponded to about 373 nucleotides, and the second corresponded to about 42 nucleotides. Inhibition of transcription of the larger product by irradiating …

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Purification Of Poly-3-Hydroxybutyrate By Density Gradient Centrifugation In Sodium Bromide, Kenneth W. Nickerson May 1982

Purification Of Poly-3-Hydroxybutyrate By Density Gradient Centrifugation In Sodium Bromide, Kenneth W. Nickerson

Papers in Microbiology

Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly- β -hydroxybutyrate. This technique generated high yields of membranebound and unbound granules of exceptional purity and degree of polymerization.

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Immunochemical Studies Of Conjugates Of Isomaltosyl Oligosaccharides To Lip I. Antigenicity Of The Glycolipids And The Production Of Specific Antibodies In Rabbits, Charles Wood, Elvin Kabat Aug 1981

Immunochemical Studies Of Conjugates Of Isomaltosyl Oligosaccharides To Lip I. Antigenicity Of The Glycolipids And The Production Of Specific Antibodies In Rabbits, Charles Wood, Elvin Kabat

Nebraska Center for Virology: Faculty Publications

The antigenicity of lipids is still ill-defined, and there is very little evidence that any pure lipid by itself will act as an antigen . To produce an immune response they must be complexed with or coupled to carriers (1-6) . Only glycosphingolipids and phosphatides have been found to function as haptens when mixed with carriers such as serum albumin (7), methylated bovine serum albumin (8), or other auxillary lipids (9-11), or when the glycolipid was incorporated into liposomes (12). Antibodies to glycolipids are usually directed primarily against the carbohydrate moiety (1, 7, 13, 14), and there is little evidence …

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Genetic Structure Of Two Species Of Waterstriders (Gerridae: Hemiptera) With Differing Degrees Of Winglessness, Anthony J. Zera Jan 1981

Genetic Structure Of Two Species Of Waterstriders (Gerridae: Hemiptera) With Differing Degrees Of Winglessness, Anthony J. Zera

Anthony Zera Publications

The Gerridae (Hemiptera: Insecta) is a worldwide family whose constituent species exhibit dramatic inter- and intra-specific variation in the degree of winglessness (Brinkhurst, 1960; Vepsäläinen, 1978; Calabrese, 1979). At one extreme, the family contains species which are fully winged in all populations and during all seasons, while several species consist almost exclusively of wingless morphs over large geographical ranges and during all seasons. Many species exhibit the intermediate case of wingpolymorphism: the occurrence of various combinations of fully winged, partially winged and/ or wingless morphs in the same population at the same time. Various wing-polymorphic species show differing patterns of …

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Conservation And Variation Of Nucleotide Sequences In Escherichia Coli Strains Isolated From Nature, Lawrence G. Harshman, Monica Riley Nov 1980

Conservation And Variation Of Nucleotide Sequences In Escherichia Coli Strains Isolated From Nature, Lawrence G. Harshman, Monica Riley

Lawrence G. Harshman Publications

A group of Escherichia coli isolates from nature were compared with one another and with laboratory strains of E. coli with respect to size distribution of chromosomal restriction endonuclease fragments and differences in nucleotide sequences in selected small portions of the genomes. The estimated frequency of base substitutions in nucleotide sequences in and near the trp operons of 26 of the 28 E. coli strains examined ranged from 0.008 to 0.066. Nucleotide sequences in or near λ prophage homologs were significantly more variable than the sequences in or near trp, tnaA, and thyA genes. Thus, the λ-homologous regions …

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Growth Of Enterobacter Cloacae In The Presence Of 25% Sodium Dodecyl Sulfate, Vance C. Kramer, Diane M. Calabrese, Kenneth W. Nickerson Nov 1980

Growth Of Enterobacter Cloacae In The Presence Of 25% Sodium Dodecyl Sulfate, Vance C. Kramer, Diane M. Calabrese, Kenneth W. Nickerson

Papers in Microbiology

The growth of Enterobacter cloacae in 25% sodium dodecyl sulfate is described. The bacteria appeared to tolerate sodium dodecyl sulfate rather than metabolize it. The process was energy dependent, and cell lysis occurred during stationary phase. Extreme detergent resistance may be characteristic of the genus Enterobacter.

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Incorporation Of Specific Fatty Acid Precursors During Spore Germination And Outgrowth In Bacillus Thuringiensis, Kenneth W. Nickerson, Lee A. Bulla Jr. Jul 1980

Incorporation Of Specific Fatty Acid Precursors During Spore Germination And Outgrowth In Bacillus Thuringiensis, Kenneth W. Nickerson, Lee A. Bulla Jr.

Papers in Microbiology

The selective incorporation of precursors specific for individual fatty acids in germinating and outgrowing spores of Bacillus thuringiensis is described. The specific precursors utilized were [14C]butyrate, -isobutyrate, -valerate, and -isovalerate, which were incorporated into even-numbered normal-chain isomers, even-numbered iso-isomers, odd-numbered normal-chain acids, and odd-numbered isohomologs, respectively. This preferential incorporation by B. thuringiensis allows the terminal carbons of specific normal and branched-chain fatty acids, contained within the cytoplasmic membrane, to be labeled with 14C and, potentially, 13C.

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Purification Of The Protein Crystal From Bacillus Thuringiensis By Zonal Gradient Centrifugation, Kenneth W. Nickerson, Barabara J. Ang Oct 1978

Purification Of The Protein Crystal From Bacillus Thuringiensis By Zonal Gradient Centrifugation, Kenneth W. Nickerson, Barabara J. Ang

Papers in Microbiology

A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.

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An Immunochemical Study Of The Combining Sites Of The Second Lectin Isolated From Bandeiraea Simplicifolia (Bs Ii), Charles Wood, E. A. Kabat, S. Ebisu, Irwin J. Goldstein Jan 1978

An Immunochemical Study Of The Combining Sites Of The Second Lectin Isolated From Bandeiraea Simplicifolia (Bs Ii), Charles Wood, E. A. Kabat, S. Ebisu, Irwin J. Goldstein

Nebraska Center for Virology: Faculty Publications

Two lectins with different binding specificities have been isolated from extracts of seeds of Bandeiraea simplicifolia. The first, Bandeiraea lectin I [11] was specific for terminal non-reducing αDGalactosyl residues. It reacted with B substances from human ovarian cysts and with several galactomannans to form precipitin lines in agar gels. Polysaccharides with terminal αDGalactosyl residues, such as larch galactan, did not react. The lectin agglutinated B erythrocytes strongly but also reacted to a lower titre with A1 and very weakly with A2 erythrocytes [15, 28] indicating that terminal non-reducing αDGalNAc [24] can …

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Absence Of Spermine In Filamentous Fungi, Kenneth W. Nickerson, Larry D. Dunkle, James L. Van Etten Jan 1977

Absence Of Spermine In Filamentous Fungi, Kenneth W. Nickerson, Larry D. Dunkle, James L. Van Etten

Papers in Microbiology

Polyamines were examined in several yeasts and filamentous fungi. Whereas putrescine, spermidine, and spennine were present in the yeasts, spernine was not detected in any of the filamentous fungi.

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Simplified Method For Efficient Intravascular Inoculation Of Chicken Embryos, Clayton L. Kelling, I. A. Schipper Jul 1976

Simplified Method For Efficient Intravascular Inoculation Of Chicken Embryos, Clayton L. Kelling, I. A. Schipper

Nebraska Center for Virology: Faculty Publications

The simple syringe-stabilizer unit described in this note provides a means for rapid intravascular inoculation of embryonated chicken eggs with minimal embryonic death from vascular trauma.

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Separation Of Spores And Parasporal Crystals Of Bacillus Thuringiensis In Gradients Of Certain X-Ray Contrasting Agentsseparation Of Spores And Parasporal Crystals Of Bacillus Thuringiensis In Gradients Of Certain X-Ray Contrasting Agents, Eugene S. Sharpe, Kenneth W. Nickerson, Lee A. Bulla Jr., John N. Aronson Dec 1975

Separation Of Spores And Parasporal Crystals Of Bacillus Thuringiensis In Gradients Of Certain X-Ray Contrasting Agentsseparation Of Spores And Parasporal Crystals Of Bacillus Thuringiensis In Gradients Of Certain X-Ray Contrasting Agents, Eugene S. Sharpe, Kenneth W. Nickerson, Lee A. Bulla Jr., John N. Aronson

Papers in Microbiology

Spores and parasporal crystals of Bacillus thuringiensis can be separated at moderate centrifugation speeds (10,000 to 12,000 rpm) in gradients of Renografin or sodium diatrizoate.

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Lipid Metabolism During Bacterial Growth, Sporulation, And Germination: Differential Synthesis Of Individual Branched And Normal-Chain Fatty Acids During Spore Germination And Outgrowth Of Bacillus Thuringiensis, Kenneth W. Nickerson, Lee A. Bulla Jr., Timothy L. Mounts Dec 1975

Lipid Metabolism During Bacterial Growth, Sporulation, And Germination: Differential Synthesis Of Individual Branched And Normal-Chain Fatty Acids During Spore Germination And Outgrowth Of Bacillus Thuringiensis, Kenneth W. Nickerson, Lee A. Bulla Jr., Timothy L. Mounts

Papers in Microbiology

The biosynthesis of individual branched- and normal-chain fatty acids during Bacillus thuringiensis spore germination and outgrowth was studied by comparing pulsed and continuous labeling of these fatty acids with [U- 14C ]acetate. The relative specific activity of each fatty acid varies with time as the cell progresses through outgrowth. However, fatty acid synthesis does occur in two distinct phases. Upon germination, acetate is incorporated only into the iso-isomers i-C13, i-C14, and i-C16; no normal or anteiso synthesis occurs. Subsequent to T30, the full complement of branched- and normal-chain homologues is formed …

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