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Articles 391 - 420 of 474

Full-Text Articles in Microbiology

Promoter Polymorphisms In Interferon Regulatory Factor 5, Daniel N. Clark Jun 2013

Promoter Polymorphisms In Interferon Regulatory Factor 5, Daniel N. Clark

Theses and Dissertations

The promoter region of interferon regulatory factor 5 (IRF5) contains the rs2004640 T or G single nucleotide polymorphism (SNP) and a CGGGG indel. Both of these polymorphisms have been implicated as genetic risk factors for several autoimmune diseases, including systemic lupus erythematosus, whose pathology involves altered apoptosis and cytokine signaling. The polymorphisms' overall effect is to increase IRF5 levels. IRF5 is a transcription factor of several cytokines, including interferon, and is pro-apoptotic. Thus an alteration of cytokine levels and apoptosis signaling due to high IRF5 levels is the proposed source of autoimmune risk. Each of IRF5's four first exons (1A, …

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Hypersaline Lake Environments Exhibit Reduced Microbial Dormancy, Joshua Christopher Vert Jun 2013

Hypersaline Lake Environments Exhibit Reduced Microbial Dormancy, Joshua Christopher Vert

Theses and Dissertations

From acid seeps and deep-sea thermal vents to glacial ice and hypersaline lakes, extreme environments contain relatively simplified communities consisting of extremophiles that have evolved to survive and thrive under adverse abiotic conditions. In more neutral environments, microorganisms use dormancy as a common life history strategy to weather temporal fluctuations of resources or stresses until more 'optimal' conditions are present. It is unclear if dormancy is an essential survival mechanism for microorganisms in extreme environments; however, recent studies suggest that extreme environments may create stable conditions for extremophiles to the extent that dormancy is of less ecological importance. Using lake …

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Thymidine Kinase 1: Diagnostic And Prognostic Significance In Malignancy, Melissa Marie Alegre Jun 2013

Thymidine Kinase 1: Diagnostic And Prognostic Significance In Malignancy, Melissa Marie Alegre

Theses and Dissertations

Thymidine kinase 1 (TK1) is a cancer biomarker which has diagnostic and prognostic potential in a variety of malignancies. TK1 is significantly elevated in the serum and tumor tissue of most malignancies. This increase in TK1 can be detected in the very early stages of malignancy, including in pre-malignant disease with an increased risk for progression. Several studies have demonstrated that elevated TK1 is found in serum months before any clinical symptoms of malignancy. It has also been demonstrated that TK1 is elevated months before clinical recurrence of malignancy. This work first sought to demonstrate the early nature of TK1 …

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Molecular Mechanisms Of Lupus Risk Conferred By Allele In The Gene For Interferon Response Factor 5, Dr. Brian Poole Apr 2013

Molecular Mechanisms Of Lupus Risk Conferred By Allele In The Gene For Interferon Response Factor 5, Dr. Brian Poole

Journal of Undergraduate Research

The academic objectives of this proposal were met. Using the funding provided by the MEG, we generated data that was used to publish four peer-reviewed journal articles and one peer-reviewed book chapter. These are:

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Transcriptional Activity Of The Novel Nuclear Protein Nbmp-2, Dr. Laura Bridgewater Apr 2013

Transcriptional Activity Of The Novel Nuclear Protein Nbmp-2, Dr. Laura Bridgewater

Journal of Undergraduate Research

The goal of this project was to follow up on preliminary data suggesting that nBmp2 directly activated transcription. A few months of follow-up experiments, however, showed that the original transcription data was not reproducible. At that point we turned our attention to two different questions about nBmp2 function: 1) Does nBMP-2 affect the cell cycle? 2) Is nBMP-2 involved in the immune response? These questions have each yielded interesting and important results that are leading toward publications and grant proposals.

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Development Of Real-Time Polymerase Chain Reaction (Q-Pcr) Assays For The Specific Detection And Characterization Of Select Bacterial Pathogens, Dr. Richard Robison Apr 2013

Development Of Real-Time Polymerase Chain Reaction (Q-Pcr) Assays For The Specific Detection And Characterization Of Select Bacterial Pathogens, Dr. Richard Robison

Journal of Undergraduate Research

Primer generation software will be used to design primer and probe sequences that will theoretically perform optimally in PCR reactions. All sequences will be subjected to BLAST searches to confirm their specificity for a particular organism. Once primer sequences have been obtained, they will be synthesized by Integrated DNA Technologies, Inc. (IDT). These primers will be evaluated in q-PCR reactions using target DNA from multiple isolates and SyberGreen to detect nucleic acid amplification. Parameters for each reaction will be optimized using these same tools. Once a reaction has been optimized, a specific labeled probe will be synthesized which contains both …

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Cytokine Signaling In The Microenvironment May Aid In Tumor Development And Progression, Dr. Kim O'Neill Apr 2013

Cytokine Signaling In The Microenvironment May Aid In Tumor Development And Progression, Dr. Kim O'Neill

Journal of Undergraduate Research

Studies have indicated that in breast tumors a high number of tumor associated macrophages (TAM’s) is indicative of a poor prognosis. TAM’s in the breast tumor microenvironment generally have a compromised immune response and focus their energy toward tissue repair which leads to angiogenesis, tumor growth, and metastasis. These macrophages have been termed M2 macrophages. Conversely, studies also indicate that in the microenvironment of some colon cancers, M1 phenotypic macrophages are present and that a high number of TAM’s is associated with a good prognosis.

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A Novel Nuclear Variant Of Nbmp-2: Role In Ca2+ Transport, Dr. Laura Bridgewater Apr 2013

A Novel Nuclear Variant Of Nbmp-2: Role In Ca2+ Transport, Dr. Laura Bridgewater

Journal of Undergraduate Research

The goal of this project was to elucidate the role that nBMP-2 plays in Ca2+ transport. In the previous year, we demonstrated that mice that have no nBMP-2 in the nuclei of their cells exhibit delays in the transport of Ca2+ back into the sarcoplasmic reticulum after each muscle contraction. During the period of this award we have extended our focus on nBMP-2 and Ca2+ transport to its effects on neurological function, since learning and memory are also dependent on intracellular Ca2+ transport. To do so, we collaborated with Dr. Jeff Edwards, a neuroscientist in the Department of Physiology and …

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Interferon Alpha In Systemic Lupus Erythematosus, Dr. Brian Poole Apr 2013

Interferon Alpha In Systemic Lupus Erythematosus, Dr. Brian Poole

Journal of Undergraduate Research

We presented data from this project at two meetings, 2 presentations at the American society for Microbiology Branch Meeting, Provo UT 2010 and one at the Intermountain Research Symposium, Logan Utah 2010. At the ASM meeting one of my undergraduates was selected to give an oral presentation, and all presentations were done by students. We also published a paper with student authorship that was supported by the MEG. The paper is “Neiwold, Timothy; Clark, Daniel; Salloum, Rafeh; Poole Brian D. Interferon alpha in systemic lupus erythematosus. J Biomed Biotechnol. 2010;2010:948364” We also have three manuscripts in preparation that include data …

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Modulators Of Symbiotic Outcome In Sinorhizobium Meliloti, Matthew B. Crook Mar 2013

Modulators Of Symbiotic Outcome In Sinorhizobium Meliloti, Matthew B. Crook

Theses and Dissertations

Microorganisms interact frequently with each other and with higher organisms. This contact and communication takes place at the molecular level. Microbial interactions with eukaryotes can be pathogenic or mutualistic. One of the best-studied symbioses is the complex interaction between nitrogen-fixing soil bacteria, termed rhizobia, and legumes. This symbiosis culminates in the elaboration of a new organ, the root nodule. Many of the molecular signals exchanged between the host plant and the invading rhizobia have been deduced, but there is still much that remains to be discovered. The molecular determinant of host range at the genus level of the plant host …

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The Function Of Yeast Pas Kinase, Dr. Julianne Grose Mar 2013

The Function Of Yeast Pas Kinase, Dr. Julianne Grose

Journal of Undergraduate Research

PAS kinase is a newly discovered member of the nutrient sensing kinases that regulates glucose homeostasis in mice and yeast. The aim of our 2009 MEG “The Function of Yeast PAS kinase” was to characterize the role of a known PAS kinase substrate, Ugp1, as well as to identify novel PAS kinase substrates. We have made significant progress on each of these aims, which has facilitated the preparation of publications, presentations and abstracts. In addition, the findings described below have opened up further research as evidenced by our 2010 and 2011 MEG proposals as well as a 2009 NIH grant …

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The Role Of Nuclear Bmp2 (Nbmp2) In Skeletal Muscle, Dr. Laura Bridgewater Mar 2013

The Role Of Nuclear Bmp2 (Nbmp2) In Skeletal Muscle, Dr. Laura Bridgewater

Journal of Undergraduate Research

The goal of this project was to characterize the molecular pathway by which nBmp2 disrupts Ca2+ handling in skeletal muscle of nBmp2 mutant mice. Previously, we had worked with Dr. Chad Hancock’s lab to measure muscle function in nBmp2 mutant mice, and those results showed a slowing in the rate at which Ca2+ is pumped from the cytoplasm back into the sarcoplamic reticulum at the conclusion of a skeletal muscle contraction. Our rationale for this MEG study was that characterizing the muscle dysfunction phenotype in the nBmp2 mutant mice would ultimately lead to a molecular level understanding of the functions …

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Evidences For Protein-Protein Interactions Between Pstb And Phou In The Phosphate Signaling Complex Of Escherichia Coli, Kristine Dawn Johns Mar 2013

Evidences For Protein-Protein Interactions Between Pstb And Phou In The Phosphate Signaling Complex Of Escherichia Coli, Kristine Dawn Johns

Theses and Dissertations

The PstSCAB2 complex serves the dual function of being a phosphate transporter as well as the primary sensor of phosphate for the Pho regulon. PhoU is an integral protein required for the signal from PstSCAB2 to be transmitted to PhoR. Our hypothesis is that conformational changes of PstSCAB2 during the phosphate transport process are the mechanism by which information about environmental phosphate levels are transduced to the cell. Additionally, we propose that direct protein-protein interactions between PhoU and the alternating conformations of PstSCAB2 mediate PhoU interactions with PhoR. By means of genetic and biochemical approaches, we have found substantial evidence …

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Effects Of Chemical Stimulation And Tumor Co-Incubation On Macrophage Activation And Aggressiveness, Measured Through Phagocytosis And Respiratory Burst, Bo Marcus Gustafsson Dec 2012

Effects Of Chemical Stimulation And Tumor Co-Incubation On Macrophage Activation And Aggressiveness, Measured Through Phagocytosis And Respiratory Burst, Bo Marcus Gustafsson

Theses and Dissertations

Macrophages are a cornerstone in innate immunity, especially important in detecting and killing invading microorganisms. In tumor biology, the macrophages can contribute both to anti-tumor activity and tumor promotion depending on individual tumor microenvironment and therefore have a large impact on both tumor progression and prognosis. Two of the most important functions of macrophages are the ability to phagocytose microorganisms and then kill them through the respiratory burst. Phagocytosis activates the respiratory burst, but the more subtle interactions between these processes are less known. Since phagocytosis and reactive oxygen species production are two attributes that change between the classically and …

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Loss Of The Lipopolysaccharide Core Biosynthesis Rfad Gene Increases Antimicrobial Chemokine Binding And Bacterial Susceptibility To Ccl28 And Polymyxin: A Model For Understanding The Interface Of Antimicrobial Chemokines And Bacterial Host Defense Avoidance Mechanisms, Cynthia S. Lew Aug 2012

Loss Of The Lipopolysaccharide Core Biosynthesis Rfad Gene Increases Antimicrobial Chemokine Binding And Bacterial Susceptibility To Ccl28 And Polymyxin: A Model For Understanding The Interface Of Antimicrobial Chemokines And Bacterial Host Defense Avoidance Mechanisms, Cynthia S. Lew

Theses and Dissertations

In order to better understand the mechanism of antimicrobial chemokine activity, including binding to and killing of bacteria, random transposon mutagenesis was performed in Yersinia pseudotuberculosis. Resulting mutants were screened for increased binding to chemokine and high binding clones were selected for further study. One mutant, designated mutant 27, was found to have a single insertion mutation in the rfaD gene. The rfaD gene product is involved in heptose biosynthesis, one of the sugars of the inner core oligosaccharide of Gram- negative lipopolysaccharide (LPS). Mutant 27 was found to bind both CCL25 and CCL28, two antimicrobial chemokines, more efficiently than …

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Characterizing The Role Of Hspb2 In Cardiac Metabolism And Muscle Structure Using Yeast And Mammalian Systems, Jonathan Paul Neubert Aug 2012

Characterizing The Role Of Hspb2 In Cardiac Metabolism And Muscle Structure Using Yeast And Mammalian Systems, Jonathan Paul Neubert

Theses and Dissertations

HspB2 is a small heat shock protein encoded on human chromosome 11. Less than 1000 base pairs away from HSPB2 and situated in a head-to-head orientation lies the gene encoding another small heat shock protein, CRYAB. Because they are uncommonly close to one another they share regulatory elements. In addition, they share protein homology as sHSPs, suggesting that they perhaps perform aimilar functions. SHSPs such as HspB2 and CryAB are traditionally thought to provide protective effects to cells in response to a variety of stress inducers. In response to stress they form complexes around misfolded proteins or proteins in danger …

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Characterization Of The Cellular And Organellar Dynamics That Occur With A Partial Depletion Of Mitochondrial Dna When Arabidopsis Organellar Dna Polymerase Ib Is Mutated, John D. Cupp Aug 2012

Characterization Of The Cellular And Organellar Dynamics That Occur With A Partial Depletion Of Mitochondrial Dna When Arabidopsis Organellar Dna Polymerase Ib Is Mutated, John D. Cupp

Theses and Dissertations

Plant mitochondrial genomes are large and complex, and the mechanisms for maintaining mitochondrial DNA (mtDNA) remain unclear. Arabidopsis thaliana has two DNA polymerase genes, polIA and polIB, that have been shown to be dual localized to mitochondria and chloroplasts but are unequally expressed within primary plant tissues involved in cell division or cell expansion. PolIB expression is observed at higher levels in both shoot and root apexes, suggesting a possible role in organelle DNA replication in rapidly dividing or expanding cells. It is proposed that both polIA and polIB are required for mtDNA replication under wild type conditions. An Arabidopsis …

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Transgene Delivery Via Microelectromechanical Systems, Aubrey Marie Mueller Wilson Aug 2012

Transgene Delivery Via Microelectromechanical Systems, Aubrey Marie Mueller Wilson

Theses and Dissertations

The invention of pronuclear microinjection initiated the field of transgenic research. Over 30 years later microinjection remains the most straight-forward and most commonly used transgene delivery option. In this work we address the current progress of microelectromechanical systems (MEMS) used as transgenic delivery mechanisms. The nanoinjector is a specially designed MEMS device which uses electrostatic charge to manipulate transgene molecules. The process of nanoinjection was designed as an alternative to microinjection which causes less damage to developing embryos, improves embryo survival, birth rates, and overall efficiency of injections. In vivo testing of nanoinjection demonstrates it is both safe and effective. …

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Effect Of Antioxidants And Oxidative Stress On Different Cancer Cell Types, Gaytri Devi Gupta Elera Jun 2012

Effect Of Antioxidants And Oxidative Stress On Different Cancer Cell Types, Gaytri Devi Gupta Elera

Theses and Dissertations

Vaccinium cyanococcus, most commonly known as blueberry, is a fruit native to North America that is known for its unique taste and high antioxidant content. The skin, seed and juice of both organically and conventionally grown blueberry extract were analyzed for antioxidant content using both the Hydrophilic and Lipophilic Oxygen Radical Absorbance Capacity (ORAC) assays. Results from the Hydrophilic ORAC test showed that conventionally grown blue berries had a higher antioxidant capacity across all samples, while the Lipophilic ORAC assay showed that the antioxidant concentration of organically cultivated blueberry juice was highest, but conventionally grown blueberry seed and skin …

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Partial Characterization Of The Antimicrobial Activity Of Ccl28, Bin Liu Feb 2012

Partial Characterization Of The Antimicrobial Activity Of Ccl28, Bin Liu

Theses and Dissertations

This research focuses on the antimicrobial activity of the mouse chemokine CCL28. In addition to their well characterized chemotactic activity, many chemokines have been shown to be antimicrobial in vitro, including the mucosally expressed chemokine CCL28. I have investigated the primary sequence features required for antimicrobial activity, salt sensitive nature of killing/binding mechanism, and in vivo microbial interactions of CCL28. Through the use of protein mutation and expression techniques, I have shown that the holoprotein (108 amino acids) is necessary for full antimicrobial activity of CCL28. Furthermore, the C terminal region of CCL28 is essential for microbial killing as an …

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Humanized Mice As A Model To Study Human Viral Pathogenesis And Novel Antiviral Drugs, Freddy Mauricio Sanchez Tumbaco Feb 2012

Humanized Mice As A Model To Study Human Viral Pathogenesis And Novel Antiviral Drugs, Freddy Mauricio Sanchez Tumbaco

Theses and Dissertations

Animal models have greatly contributed to the understanding of different aspects of human biology, as well as a variety of human-related pathogens and diseases. In order to study them, humanized mice susceptible to pathogens that replicate in human immune cells have been developed (e.g., humanized Rag2-/-γc-/- mice). These animals are engrafted with human hematopoietic stem cells (HSCs), resulting in the de novo development and maturation of the major functional components of the human adaptive immune system and the production of a variety of human cell types. Primary and secondary lymphoid organs in the mouse are populated with human cells, and …

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Characterization Of The Role Nuclear Bmp2 (Nbmp2) Plays In Regulating Gene Expression, Fialka Grigorova Dec 2011

Characterization Of The Role Nuclear Bmp2 (Nbmp2) Plays In Regulating Gene Expression, Fialka Grigorova

Theses and Dissertations

The nBmp2 protein was first identified in a DNA affinity chromatography/mass spectrometry screen designed to detect proteins that interact with a cartilage-specific enhancer element (called D/E) from the type XI collagen gene Col11a2. The transcription factor SOX9, a protein from the Sox (SRY-related HMG box) family, binds to and activates gene expression from this enhancer. nBmp2 has no transcriptional activity of its own on this enhancer, but when co-transfected with SOX9 it increases SOX9's activation of D/E nearly 2-fold. SOX9 also activates cartilage-specific enhancer elements from the Col2a1, Col27a1, and Col9a1 genes. The purpose of this project …

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Antioxidants In Cancer Research And Prevention: Assay Comparison, Structure-Function Analysis, And Food Product Analysis, Andrew Robert Garrett Jun 2011

Antioxidants In Cancer Research And Prevention: Assay Comparison, Structure-Function Analysis, And Food Product Analysis, Andrew Robert Garrett

Theses and Dissertations

Recent epidemiological studies have suggested that the development and progression of several chronic diseases may be initiated or augmented by oxidative stress. Reactive oxygen species and reactive nitrogen species react readily with and can damage nucleic acids, proteins, and lipids. While biological systems are equipped antioxidant defenses to cope with oxidative stress, oxidative damage may still occur when oxidative stress overwhelms antioxidant defenses. This damage, if left unchecked, may lead to a variety of degenerative diseases, including heart disease, Alzheimer's Disease, Parkinson's Disease and cancer. Several assays have been designed to describe the antioxidant activity of various phytochemicals, vitamins, and …

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Effects Of Diabetic State And Gender On Pro-Inflammatory Cytokine Secretion By Human Macrophages Infected With Burkholderia Pseudomallei, Annette J. Blam Nov 2010

Effects Of Diabetic State And Gender On Pro-Inflammatory Cytokine Secretion By Human Macrophages Infected With Burkholderia Pseudomallei, Annette J. Blam

Theses and Dissertations

Burkholderia pseudomallei is a gram-negative opportunistic soil pathogen that causes the life-threatening disease melioidosis. It is endemic in Northern Australia and Southeast Asia but can be found throughout many other regions in the world. Diabetes mellitus is a predisposing risk factor for infection with this organism and it has been demonstrated that diabetic males are particularly susceptible to severe infection. Previous research suggested that monocytes isolated from the whole blood of diabetic males demonstrated a decreased ability to produce the proinflammatory cytokines IL-1β and IL-8. We hypothesized that monocyte-derived macrophages from diabetic males would also secrete lower levels of pro-inflammatory …

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Determining The Function Of Nuclear Bmp4, Trina Jane Loos Aug 2010

Determining The Function Of Nuclear Bmp4, Trina Jane Loos

Theses and Dissertations

Bone morphogenetic protein 4 (Bmp4) is a well known growth factor that regulates gene expression through the SMAD signaling pathway. Bmp4 is involved in many developmental processes and has been identified as an important factor in several cancers, including melanoma, ovarian cancer, and colon cancer. Madoz-Gurpide et al. recently observed Bmp4 in the nuclei of a minor percentage of cells in colon cancer tissues. In addition, our lab has recently discovered a nuclear variant of Bmp2 (nBmp2), the TGF-β family member most closely related to Bmp4. These observations led us to hypothesize that a nuclear variant of Bmp4 (nBmp4) also …

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Interactions Of Burkholderia Pseudomallei And Acanthamoeba Castellanii And Their Effects On Virulence In Human Monocytes, Emily Ann Moore Jul 2010

Interactions Of Burkholderia Pseudomallei And Acanthamoeba Castellanii And Their Effects On Virulence In Human Monocytes, Emily Ann Moore

Theses and Dissertations

Burkholderia pseudomallei, the etiological agent of melioidosis, is a saprophytic bacterium existing endemically in the water and soil of SE Asia and Northern Australia. This organism has shown the ability to remain dormant in its host for decades. B. thailandensis is a closely related non-pathogenic near neighbor that is also found in these soils. It has been suggested that free-living amoeba could be natural reservoirs for these organisms. The interactions of Burkholderia species and Acanthamoeba castellanii, a species of free-living amoeba, were studied to better understand the natural ecology of these organisms and to determine the effects amoeba …

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Organellar Dna Polymerases Gamma I And Ii In Arabidopsis Thaliana, Jeffrey M. Brammer Jun 2010

Organellar Dna Polymerases Gamma I And Ii In Arabidopsis Thaliana, Jeffrey M. Brammer

Theses and Dissertations

Plants have two organelles outside the nucleus which carry their own DNA, mitochondria and chloroplasts. These organelles are descendants of bacteria that were engulfed by their host according to the endosymbiotic theory. Over time, DNA has been exchanged between these organelles and the nucleus. Two polymerases, DNA Polymerases Gamma I and II, are encoded in the nucleus and remain under nuclear control, but are transported into the mitochondria and chloroplasts. DNA polymerases gamma I and II are two organelle polymerases which have been studied through sequence analysis and shown to localize to both mitochondria and chloroplasts. Little has been done …

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Gene Expression Patterns In Flea Vectors Of Yersinia Pestis, Wei Zhou Mar 2010

Gene Expression Patterns In Flea Vectors Of Yersinia Pestis, Wei Zhou

Theses and Dissertations

Plague bacteria (Yersinia pestis) are transmitted to susceptible mammals by fleas. At least 25 flea species found in North America have been identified as plague vectors. The most efficient flea vector is the Oriental rat flea Xenopsylla cheopis, while the cat flea Ctenocephalides felis is a poor vector. The factors that determine vector competence of different fleas are not known. The main obstacles that the bacteria must overcome in the flea gut are also unknown. Fleas' molecular responses to Y. pestis invading could be a determining factor to control the bacterial survival and growth. Good and poor …

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Bpv Entry And Trafficking In Ebtr Cells, Enkhmart Dudleenamjil Nov 2009

Bpv Entry And Trafficking In Ebtr Cells, Enkhmart Dudleenamjil

Theses and Dissertations

Bovine Parvovirus (BPV) belongs to the genus Bocavirus, family Parvoviridae. BPV is the leading etiologic agent among the pathogens that cause primary gastroenteritis of cattle. Many of the intracellular events associated with virus replication are unknown. In this research project, we investigated BPV internalization into the host cell and trafficking in the cytosol. Preliminarily, EBTr cells had abundant clathrin, virus attached to purified clathrin, and EM micrographs revealed virus in endocytic vacuoles. Assays detecting virus infectivity (i.e. viral protein synthesis), virus production (completion of the replication cycle), and quantitative PCR (qPCR) to detect viral transcripts were used to evaluate …

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Part 1: Screening Of Thirty-One Medicinal Plant Species Against Herpes Simplex Virus, Acetone And Methanol Extracts From The Root Tissue Of Kalanchoe Pinnata Interferes With Hsv Types 1 And 2 Dna Replication And Early And Late Gene Expression Preventing The Spread Of Hsv In Vitro. Part 2: Professional Development Curriculum: Integrating Molecular Biology And Microbiology Into The Existing Secondary Biology Curricula, Mary Ruth Greer Nov 2009

Part 1: Screening Of Thirty-One Medicinal Plant Species Against Herpes Simplex Virus, Acetone And Methanol Extracts From The Root Tissue Of Kalanchoe Pinnata Interferes With Hsv Types 1 And 2 Dna Replication And Early And Late Gene Expression Preventing The Spread Of Hsv In Vitro. Part 2: Professional Development Curriculum: Integrating Molecular Biology And Microbiology Into The Existing Secondary Biology Curricula, Mary Ruth Greer

Theses and Dissertations

PART 1: Thirty-one medicinal plant species from Hawaii, Morocco, and the Sonoran Desert, USA have been shown in past studies to be highly inhibitory to pathogenic bacteria, fungi, and certain cancer cell lines. However, none were tested for antiviral activity. Acetone and methanol extracts from these species were bio-assayed for antiviral activity against herpes simplex virus types 1 and 2 (HSV 1 and HSV 2) and for cytotoxicity to the Vero C1008 cell line. Extracts from these species were tested in vitro for antiviral activity using an immunoperoxidase mini-plaque reduction assay to detect viral structural protein synthesis. Sulforhodamine B and …

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