Microbiology Commons^™

Isolation Of Plastic-Degrading Fungi From Landfill And Determining The Selected Plastics Biodegradation Capability, Mohan Priya

Student Works (2020-2029)

Polyethylene Terephthalate (PET), Low Density Polyethylene (LDPE) and High Density Polyethylene (HDPE) are the most important polymers used predominantly in present world. Since synthetic polymers hardly undergo degradation and remain in the environment for a very long time, up to 1000 years, these drawbacks increase the percentage of solid wastes in landfill and threaten the environment. To date, Municipal Solid Waste (MSW) landfill is the most favored disposal method especially among the developing countries. The aim of present study is to evaluate the potential of fungus isolated from landfilled plastics to degrade PET, LDPE and HDPE. Besides, effect of different …

Identification Of A Malaysian Tiger Milk Mushroom Specimen And Characterisation Of Its Mycelial (1,3)-Β-D-Glucan, Ahmad Usuldin Siti Rokhiyah

Student Works (2020-2029)

The tiger milk mushroom (Lignosus rhinocerus) is an important medicinal mushroom and regarded as Malaysia national heritage. Its sclerotia (tuber) has been consumed by the indigenous community (Orang Asli) to treat illnesses such as asthma, fever, cough, cancer and other diseases. The mushroom is rare and difficult to find in nature and has frequently been confused with another mushroom of similar anatomy. Traditionally, the cultivation of the sclerotium takes a long time and propagation of its mycelial biomass and exopolysaccharides (EPS) in a stirred-tank bioreactor is an excellent alternative technique. The present study aimed to identify the wild-Malaysian tiger milk …

The Effects Of An Applied Phyllosphere-Microbiome On Gas Exchange And Growth Of Soybean Infected With Pseudomonas Syringae: Harnessing The Power Of The Microbiome, Charles Agbavor

Graduate Theses/Dissertations

The microbiome is an important determinant of plant health, growth and resistance to stress. This study was conducted to determine the efficacy of the phyllosphere-microbiome on managing Pseudomonas syringae pv. Glycinea (Psg), gas exchange and growth of Glycine max L. Merrill. A greenhouse study was conducted in the summer of 2019. A field isolated and cultured soybean phyllosphere-microbiome was applied to purposely infected and uninfected soybean. Gas exchange, Psg density, chlorophyll concentrations, and percent nitrogen and carbon in the leaves were measured during the V3-V6 stages of soybean. Not surprisingly, there was a two-fold reduction in copies of Pseudomonas …

Investigation Of An Elevational Gradient Reveals Strong Differences Between Bacterial And Eukaryotic Communities Coinhabiting Nepenthes Phytotelmata, Kadeem J. Gilbert, Leonora S. Bittleston, Mark Arcebal K. Naive, Anthony E. Kiszewski, Perry Archival C. Buenavente, David J. Lohman, Naomi E. Pierce

Publications and Research

Elevation is an important determinant of ecological community composition. It integrates several abiotic features and leads to strong, repeatable patterns of community structure, including changes in the abundance and richness of numerous taxa. However, the influence of elevational gradients on microbes is understudied relative to plants and animals. To compare the influence of elevation on multiple taxa simultaneously, we sampled phytotelm communities within a tropical pitcher plant (Nepenthes mindanaoensis) along a gradient from 400 to 1200 m a.s.l. We use a combination of metabarcoding and physical counts to assess diversity and richness of bacteria, micro-eukaryotes, and arthropods, and compare the …

Mechanisms Of Silencing Suppression By A Polerovirus P0 Protein, Natalie Holste, Hernan Garcia-Ruiz

School of Biological Sciences: Presentations

Maize lethal necrosis is an intense viral disease spreading across sub-Saharan Africa. Maize is the staple crop grown in sub-Saharan Africa, but most crops infected with maize lethal necrosis will not survive to harvest. This causes immense economic hardship and starvation within the population. Maize lethal necrosis consists of a combination of two viruses, Maize chlorotic mottle virus (MCMV) and a virus from the genus potyvirus. In a recent study, a Maize yellow dwarf virus-RMV (MYDV-RMV)-like polerovirus, was repeatedly detected in plants with maize lethal necrosis. Poleroviruses have a silencing suppressor, P0 protein, and the mechanism of suppression is poorly …

Fungicide Sensitivity Of Sclerotinia Sclerotiorum Isolates Selected From Five Different States That Use Different Fungicide Treatments, Cristian Wulkop Gil

UCARE: Research Products

Sclerotinia sclerotiorum is a plant pathogenic fungus that causes a disease called white mold that can infect more than 450 plant species including soybeans, dry beans, green beans, canola, and sunflower. This pathogen is capable of up to $252M in losses every year (U.S. Canola Association, 2014). Fungicides are widely used in developed agricultural systems to control disease. However, resistance to the most effective fungicides has emerged and spread in pathogen populations and there have been multiple reports of S. sclerotiorum isolates becoming resistant to certain fungicides. Since different fields in different states use different fungicide treatments on plants and …

Life Line March 2020, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

The Department Welcomes Dr. Sinn; Modeling to Help with Global Research; The Paradise of Bombs Revisited; The End of an Era in Conesville Ohio; Reports from two recipients of the Melinda Phinney Award for Pre-Med Travel Experiences; Preparing Skins for Study; Genomics in the Jungle; Zoo Program Gets New T -Shirt and Sweatshirt Designs; Aquarium Track Dives Right In; Dr. Svitana’s Sabbatical Accomplishments;Biology Behind Bars; Grossology: The Science of Icky, Sticky, Disgusting Things.

Gene Expression Analysis Of Effectors Involved In Early Interaction Of Banana And Fusarium Oxysporum F. Sp. Cubense, Nee Kiew Poon

Student Works (2020-2029)

Banana is a popular but disease-laden fruit crop. It is very susceptible to various phytopathogens such as viruses, bacteria, fungi and nematodes. A particularly infectious and economically important fungal disease in banana is Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). The disease has been plaguing the banana industry since late 19th century. Despite the early discovery of this phytopathogen of banana, little is known on its the mechanism of pathogenicity. In other plants, fungal effectors have been found to function in manipulating plant mechanisms during infection and many have been identified and characterized. In this study, the …

In Vitro Regeneration Of Dioscorea Alata With Anthocyanin And Antimicrobial Activity, Abdullah Sakinah

Student Works (2020-2029)

An efficient protocol was developed for rapid propagation and regeneration of the Dioscorea alata L. locally known as ‘ubi badak’ because of their rhinoceros-shaped tubers. In the present study, direct and indirect regeneration were induced to produce plantlets of Dioscorea alata L. For direct regeneration explants (leave, stem and node) were cultured on MS media supplemented with various combinations and concentrations of BAP and NAA to induce microshoots and roots formation. The optimum medium for microshoots formation was MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l NAA with the number of microshoot is 23.07±0.44 from node explant. While …

The Influences Of Green Infrastructure On Levels Of Somatic And Male-Specific Coliphages At Shadyside Park, Oula Salih

Student Papers and Reports

Microbial contamination is still considered one of the major causes of diminished function and quality of urban rivers and streams in Michigan. Floodplains, a type of green infrastructure used to reduce flooding after large amounts of rainfall, were reported to have variable effects of reducing levels of stormwater runoff within these bodies of water ( Hubbart et al., 2011 ). Viral indicators of fecal contamination in bodies of water include male-specific and somatic coliphages. Water samples from Plaster Creek were collected before and after the floodplain in order to identify the levels of somatic and male-specific coliphages during dry and …

Microbial Degradation Of Urea Oligomers: Potential Slow Release Nitrogen Fertilizers, Maddy Bygd

Departmental Honors Projects

Urea is the most commonly used nitrogen compound in fertilizers across the world. It provides the most nitrogen at the lowest cost. However, with the size and solubility of this compound, it is easily washed out of the soil. This results in the need to continuously add fertilizer to fields, creating adverse effects on the environment due to nitrogen runoff and leading to increased expenses for farmers. Triuret, a urea oligomer, is a compound with promising potential for use as a nitrogen source, providing a slower release of nitrogen into the soil. It is hypothesized that this compound can be …

Evaluation Of The Symbiotic Relation Between Endophyte And Poplar Trees Exposed To Landfill Leachate, Catalina Vega Hurtado

Masters Theses

“The contamination of soil and groundwater from leachate leakage from landfill has turned, nowadays, into a global public issue. One of the main concerns about this pollutant is the potential threat to human and ecosystem health. The current research studied endophyte bacteria in association with poplar trees (Populus sp.), as a method of bioremediation of landfill leachates. The objective of the project was to identify treatment strategies that may improve plant performance (survival rates, plant fitness, and degradation efficacy) with the purpose of being implemented in phytoremediation plots, aimed to intercept and treat landfill leachate, before the contamination of watershed. …

Virus-Induced Changes In Nuclear Proteins And Membranes In Nicotiana Benthamiana Cells, Caleb Mathias

Theses and Dissertations--Plant Pathology

Viruses rely on host proteins to complete their life cycles. This results in alterations to normal cell physiology to processes which benefit viral processes such as replication and movement to other cells. This may involve the relocalization of host proteins away from their original subcellular targets to sites which may benefit the virus, a process that is not as well understood as it relates to the nucleus. To identify nuclear proteins that may be involved in such processes, a library of random Nicotiana benthamiana cDNAs were expressed as GFP fusions in a transgenic marker lines expressing a histone 2B:RFP nuclear …

Developing Microbial Based Process To Produce High Value Natural Antimicrobial (Glyceollin) In Soybeans And Pullulan From Sucrose, Andrea Zavadil

Electronic Theses and Dissertations

Glyceollin is a secondary metabolite produced under stress conditions by soybean to act as a plant defender to pathogen attack. The antimicrobial nature of glyceollin makes it a promising natural alternative to antibiotics if incorporated into livestock feed. However, production of glyceollin is highly variable, dependent on soybean variety, and fungal elicitor used as inoculum. Our study compares two fungal spore elicitors, Trichoderma reesei and Aspergillus sojae on soybeans of diverse maturity level, and fungal susceptibility to determine highest glyceollin titer. Spore inoculation with T. reesei elicited highest glyceollin titers of 2.42±0.20 mg/g at 120 h, while A. sojae elicited …

Investigating Links Between Soil Microbial Structure And Function In Three Major Plant Communities Across Temporal Scales Of Arctic Alaska, Kaj Lynoe

Electronic Theses and Dissertations

Arctic microbial systems continue to get attention today as our understanding regarding their structure and function in a changing system is paramount to C feedbacks with warming and changes in precipitation. Plant communities and microbial community processes across the Arctic landscape are central to understanding tundra ecosystem processes because environmental conditions and plant community structure drive microbial cycling of soil organic matter. Here, we want to understand how soil microbial respiration, mineralization, biomass, and community composition are linked to three Alaskan tundra plant communities, namely Shrub, Tussock, and Sedge tundra and the seasonal variability in this system. A total of …

Utilizing Rhizospheric And Bacterial Endophytes For Use As Potential Bio-Fertilizers For Sustainable Agricultural Production, Vincent Peta

Electronic Theses and Dissertations

Brassica carinata crop production offers an exciting alternative approach to the energy crisis when looking at novel ways to power our motor vehicles. Bacterial endophytes, while residing in host-plant tissues, offer a wide array of plant growth promotion benefits such as nitrogen fixation to impart nitrogen (N) nutrition, phosphate (P) solubilization to make soil-bound phosphate more mobile in soil for plant uptake, can suppress pathogenic microbes such as fungus and produce plant growth hormones to offset stress incurred by the plant. Previous literature has shown that there is large interest in studying endophytes from economically important crops such as wheat, …

Characterization Of Bacterial Endophytes Isolated From Brassica Carinata And Their Potential Use To Decrease Nutrient Requirements In Crops, Alex Soupir

Electronic Theses and Dissertations

Bacterial endophytes have the capability to enhance plant growth by producing plant growth hormones, solubilizing phosphates, suppressing pathogenic fungi, and reducing plant stress hormones. These capabilities make them desirable limiting the amount of nutrients and pesticides that are applied to crops. Through these assays and isolations, it is possible to identify novel bacterial species. In-vitro testing had shown 9 of the 20 isolates possess the ability to produce indole-3-acetic acid (IAA) with Pantoea agglomerans BC09 producing a concentration of 30.2 ng/μl over 4 days. BC09, Bacillus subilis BC10, and Pantoea sp. BC12 were able to solubilize calcium phosphate, 7 endophytes …

Antibacterial, Antifungal, Antioxidant Activity And Polyphenol Content Of Aerial Parts And Bulbs Of Allium Schugnanicum., Saidbeg Satorov, F. Mirzoeva, Sh. Kurbonbekova, Sh. Satorov, M. Vakhidova, Vyacheslav Dushenkov

Publications and Research

Objective: То study of antibacterial, antifungal and antioxidant activity, and content of polyphenols in the bulb and aerial parts of an endemic species of onion A. schugnanicum. Methods: An ethanol-based extract was prepared from fresh plants. Total polyphenolic content (Folin) and ABTS antioxidant capacity assays were used to characterized extracts. Extracts obtained from bulbs, peduncle, and seeds demonstrated antibacterial activity against the reference Staphylococcus aureus (ATCC 4929), Pseudomonas aeruginosa (ATCC 4930) and Klebsiella pneumoniae (ATCC 4927) strains, as well as against the hospital strains of the same types of bacteria, i.e. strains isolated from inpatient surgical patients. Results: The study …

Genetic And Biochemical Characterization Of Ergot Alkaloid Synthesizing Fungi And Their Symbionts, Matthew Duane Maust

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ergot alkaloids are fungal tryptophan derived toxins which affect mammalian circulation and neurotransmission. These compounds are biosynthesized by a conserved genetic pathway, known as the ergot alkaloid synthesis (EAS) pathway by fungi belonging to the ascomycete families Trichocomaceae and Clavicipitaceae. Several Ipomoea species and related plants in the morning glory family harbor vertically transmitted symbiotic fungi in the genus Periglandula, also members of Clavicipitaceae, that produce ergot alkaloids. Metabolomic analysis of seeds identified a previously uncharacterized glycoside form of the pharmaceutically important ergot alkaloid, ergonovine. Several species belonging to the fungal genus Metarhizium have recently been shown to have the …

Pertumbuhan, Hasil Dan Karakter Fisiologis Padi Yang Diberi Pupuk Hayati Pada Pertanaman Kelapa Sawit Belum Menghasilkan I, Annisa Nuraisah, Cucu Suherman, Mira Ariyanti, Anne Nuraini, Mochamad Arief Soleh

Jurnal Kultivasi

ruang terbuka diantara tanaman kelapa sawit belum menghasilkan dapat dimanfaatkan untuk budidaya tanaman pangan, khususnya padi sebagai tanaman sela dalam rangka mendukung program ketahanan nasional. Penelitian dilakukan dengan tujuan untuk memperoleh dosis pupuk hayati yang berpengaruh paling baik terhadap pertumbuhan, hasil, dan karakter fisiologis tanaman padi yang ditanam pada pertanaman kelapa sawit TBM 1 serta melihat dampak penanaman padi sebagai tanaman sela terhadap pertumbuhan kelapa sawit. Percobaan dilakukan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, pada bulan Desember 2017 sampai bulan April 2018. Percobaan disusun menggunakan Rancangan Acak Kelompok (RAK) yang terdiri dari enam perlakuan dosis pupuk hayati, yaitu …

Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda E. Shine

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nutrient inputs from cattle dung are crucial drivers of nutrient cycling processes in grazed ecosystems. These inputs are important both spatially and temporally and are affected by variables such as grazing strategy, water location, and the nutritional profile of forage being grazed. Past research has attempted to map dung deposition patterns in order to more accurately estimate nutrient input, but the large spatial extent of a typical pasture and the tedious nature of identifying and mapping individual dung pats has prohibited the development of a time- and cost-effective methodology. The first objective of this research was to develop and validate …

Phenotypic Study Of Anthracnose Resistance In Black Walnut And Building A Mapping Population, Sadie D. Land

Graduate Theses/Dissertations

Black walnut anthracnose, caused by Gnomonia leptostyla, is the most widespread and destructive disease affecting black walnut trees (Juglans nigra). Breeding cultivars for a higher resistance to anthracnose is a natural and efficient strategy for improving the health and production quality of black walnut trees. The two goals of this study were to reveal that the ‘Sparrow’ cultivar of black walnut contains a significantly higher resistance to anthracnose than the ‘Football’ cultivar when separated from environmental factors, and to expand the ‘Football’ × ‘Sparrow’ F1 mapping population to evaluate how the trait of resistance is inherited in the progeny. A …

Coffee Is Not Forever : A Global History Of The Coffee Leaf Rust, Stuart Mccook

Ohio University Press Open Access Books

The global coffee industry, which fuels the livelihoods of farmers, entrepreneurs, and consumers around the world, rests on fragile ecological foundations. In Coffee Is Not Forever, Stuart McCook explores the transnational story of this essential crop through a history of one of its most devastating diseases, the coffee leaf rust. He deftly synthesizes agricultural, social, and economic histories with plant genetics and plant pathology to investigate the increasing interdependence of the world’s coffee-producing zones. In the process, he illuminates the progress and prognosis of the challenges—especially climate change—that pose an existential threat to a crop that global consumers often take …

Dinamika P-Tersedia Pada Limbah Cair Kelapa Sawit Dengan Beberapa Land Application, Koko Tampubolon, Melina Vika, Debora

Jurnal Kultivasi

Ketersediaan P sangat rendah pada land application perkebunan kelapa sawit di Sumatera Utara dibandingkan unsur N dan K. Tujuan penelitian ini untuk mengetahui ketersediaan P yang terdapat pada land application, hubungannya terhadap produksi TBS, serta membandingkan dinamika P-tersedia beberapa perkebunan kelapa sawit lainnya. Penelitian ini dilaksanakan dengan pengambilan sampel tanah land application pada Blok A88B dan A88E Afdeling 1 Kebun Teluk Panji, Kecamatan Kampung Rakyat Kabupaten Labuhanbatu Selatan, Sumatera Utara. Analisis tanah dilakukan di Laboratorium Kesuburan Tanah, Fakultas Pertanian Universitas Sumatera Utara dan Laboratorium Analitik dan Quality Control Asian Agri, Tebing Tinggi. Penelitian ini dilaksanakan pada bulan Juni sampai dengan …

Respons Pertumbuhan, Hasil, Dan Tingkat Kerebahan Padi Varietas Ipb 3s Terhadap Pupuk Hayati Dan Nano Silika, Lia Marliani, Sumadi, Tati Nurmala

Jurnal Kultivasi

Peningkatan produktivitas tanaman pangan merupakan salah satu upaya dalam mendukung ketahanan pangan seiring dengan bertambahnya jumlah penduduk di Indonesia. Penggunaan pupuk hayati dan nano silika secara mandiri mampu meningkatkan pertumbuhan dan hasil tanaman serta menurunkan tingkat kerebahan tanaman padi. Penelitian ini bertujuan untuk mendapatkan kombinasi dosis pupuk hayati dan nano silika yang tepat sehingga dapat meningkatkan pertumbuhan, hasil, dan ketegaran tanaman padi sawah (Oryza sativa L) varietas IPB 3S. Penelitian ini dilaksanakan di lahan sawah yang berlokasi di Kampung Bojongloa, Desa Tegalsawah, Kecamatan Karawang Timur, Kabupaten Karawang pada bulan Maret sampai dengan bulan Juli 2017. Rancangan percobaan yang digunakan adalah …

Bottom-Up Controls (Micronutrients And N And P Species) Better Predict Cyanobacterial Abundances In Harmful Algal Blooms Than Top-Down Controls (Grazers), Scott Andrew Collins

Theses and Dissertations

The initiation, bloom, and bust of harmful Cyanobacteria and algae blooms (HAB) in lakes are controlled by top-down and bottom-up ecological controls. Excess phosphorous and nitrogen inputs from anthropogenic sources are primary to blame, but eukaryotic grazers may also promote or curb Cyanobacteria dominance. We tracked shifts in bacterial composition, lake chemistry, and eukaryotic grazing community weekly or bi-weekly through spring and summer and modeled the causes of specific Cyanobacterial species blooms and busts across three lakes in Utah, USA, with differing lake trophic states. Regardless of trophic status, all three lakes experienced blooms of varying composition and duration. Aphanizomenon …

Biomethane Production From Distillery Wastewater, Zachary Christman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Distillery wastewater treatment is a great ecological problem, for example, India produces 2.7 billion liters of alcohol that results in 40 billion liters of wastewater. However, this material can be seen as a resource since 11 million cubic meters of biogas at 60% methane could be produced in addition to cleaning the water. The distillery has two options of what to do with the biogas. The first is to use the biogas to fuel the distillery making the production plant more energy efficient and removing some of the need to buy natural gas. The other is to upgrade the biogas …

Warming Up: Climate Change Related Shifts Of Mycorrhizal Fungal Communities In High Latitude Ecosystems, Megan Rae Devan

Biology ETDs

This dissertation examines how climate change affects mycorrhizal fungal communities in boreal and arctic ecosystems. In chapter one, I revealed that increases in fire severity and related increases in deciduous tree dominance result in greater Ascomycota relative abundance (RA) and subsequent declines in Basidiomycota RA. In chapter two I analyzed the effects of post-fire mycorrhizal fungal communites on host growth. There were trends at the fungal genus level that were largely reflected at the guild level across all hosts; however, there were some fungal genera that had the opposite effect on different host species. In chapter three, I found host …

Screening Of Crude Microalgal Extracts For Antimicrobial Activity, Patrick Matulich

Theses & Dissertations

Microbial biofilm associated infections are a significant threat to patients with medical devices and are partially responsible for the increased resistance seen in nosocomial infections. The biofilm chemical and physical properties restrict access of chemotherapeutic agents. Therefore, there is a need to enhance the antimicrobial effects of current chemotherapeutic agents. Microalgae live in competitive environments that include film-forming, opportunistic pathogens S. maltophilia and C. albicans. Extracts from 5 diverse species of microalgae were screened for antimicrobial enhancing effects against established biofilms with two different extraction solvents, 3:1 hexanes and isopropyl (HIPA) and 2-methyltetrahydrofuran (2-MTHF).

Three species, B. braunii, …

Reporting Charcoal Rot In Chia And Developing A Susceptibility Assay, Reis M. Misaka, Hagop S. Atamian Dr., Julien Besnard Dr.

Student Scholar Symposium Abstracts and Posters

Chia (Salvia Hispanica) cross breeds were planted in the summer of 2018 with the intent of selective breeding for agricultural benefit. Preexisting pathogens in the soil caused 40-50% fatality of adult plants. This was surprising due to the precursory knowledge that chia has antibiotic and antifungal oils (Elshafie et. al. 2018); chia was only recently documented to be susceptible to Fusarium wilt (Fusarium oxysporum). The primary pathogen responsible was identified as Macrophomina phaseolina (aka charcoal rot); a widespread soilborne pathogen which has multiple commercial hosts (Su et. al. 2001).

M. phaesolina on wheat seed vector where used as …