Microbiology Commons^™

Comparative Genomics And Virulence Studies Of Streptomyces Soil Rot And Scab Pathogen Species, Natasha Soares

LSU Doctoral Dissertations

Comparative genomic analyses were performed to gain insights into the organization and content of the genome of Streptomyces ipomoeae, the soil rot pathogen that infects sweetpotatoes. Unlike Streptomyces scab pathogens, the thaxtomin phytotoxin gene cluster (txt) in S. ipomoeae does not appear to reside within a genomic island and has diverged from its scab pathogen counterparts. Increased usage of the rare TTA codon, particularly for the txt cluster, suggests greater translational control by the bldA tRNA in S. ipomoeae. Orthologous gene searches and secondary metabolite profiling yielded ortholog groups and metabolite gene clusters that were exclusive …

Formulasi Pembawa Rizobakteri Penambat Nitrogen Dan Pelarut Fosfat, Serta Aplikasinya Di Pembibitan Kelapa Sawit Yang Diberi Komposisi Dan Dosis Amelioran Yang Berbeda, Roby Ibnu Syarifain, Mohammad Raihan Rashaun, Eka Dewi Anggrainy, Tualar Simarmata

Jurnal Kultivasi

Keberhasilan aplikasi pupuk hayati berkaitan dengan viabilitas mikroba dalam pembawa, dan aplikasinya di lapangan. Penelitian ini bertujuan mengetahui viabilitas Bakteri Penambat Nitrogen (BPN) dan Bakteri Pelarut Fosfat (BPF) pada berbagai komposisi karier, konsentrasi molase, dan aplikasinya dengan berbagai dosis serta komposisi amelioran pada pembibitan kelapa sawit. Penelitian dilaksanakan dari bulan Oktober 2019 - Mei 2020 di laboratorium dan kebun percobaan Ciparanje. Tahap pertama menggunakan Rancangan Acak Lengkap dua faktor (komposisi karier dan konsentrasi molase) dengan tiga ulangan. Tahap kedua menggunakan rancangan acak kelompok faktorial dua faktor (komposisi amelioran dan dosis aplikasi) pada pembibitan kelapa sawit sebanyak tiga ulangan. Hasil riset …

Uji Kemampuan Bakteri Diazotrof Asal Perakaran Bawang Merah Dalam Mendukung Pertumbuhan Dan Hasil Tanaman Bawang Merah, Ananda Rahmalia Yuniarti, Eny Rokhminarsih, Purwanto

Jurnal Kultivasi

Budidaya tanaman bawang merah saat ini dihadapkan pada masalah stagnasi produksi akibat aplikasi pupuk kimia secara berlebihan sehngga efisiensi pemupukan rendah. Teknologi ramah lingkungan dan efisien pupuk dengan aplikasi bioteknologi melalui aplikasi bakteri berguna seperti bakteri penambat nitrogen. Penelitian bertujuan untuk (1) mengetahui respons pertumbuhan dan hasil tanaman bawang merah terhadap bakteri diazotrof, (2) menetapkan isolat bakteri diazotrof yang paling baik untuk pertumbuhan dan hasil tanaman bawang merah. Percobaan dilaksanakan pada bulan Januari 2021 sampai April 2021. Percobaan dilaksanakan di Rumah Kassa serta Laboratorium Agronomi dan Hortikultura Fakultas Pertanian, Universitas Jenderal Soedirman. Rancangan percobaan yang digunakan yaitu Rancangan Acak Kelompok …

Increasing The Resilience Of Plant Immunity To A Warming Climate, Jong Hum Kim, Christian Castroverde, Shuai Huang, Chao Li, Richard Hilleary, Adam Seroka, Reza Sohrabi, Diana Medina-Yerena, Bethany Huot, Jie Wang, Sharon Marr, Mary Wildermuth, Tao Chen, John Macmicking, Sheng Yang He

Biology Faculty Publications

Extreme weather conditions associated with climate change affect many aspects of plant and animal life, including the response to infectious diseases. Production of salicylic acid (SA), a central plant defence hormone, is particularly vulnerable to suppression by short periods of hot weather above the normal plant growth temperature range via an unknown mechanism. Here we show that suppression of SA production in Arabidopsis thaliana at 28 °C is independent of PHYTOCHROME B (phyB) and EARLY FLOWERING 3 (ELF3), which regulate thermo-responsive plant growth and development. Instead, we found that formation of GUANYLATE BINDING PROTEIN-LIKE 3 (GBPL3) defence-activated biomolecular condensates (GDACs) …

Native And Non-Native Ant Impacts On Soil Microbes, Hannah A. Stewart

Biology Theses

Organisms produce chemical weapons for defense, but target organisms can develop resistance. In their introduced range, non-native species may bring “novel weapons” against which native organisms have not co-evolved resistance. The invasive European fire ant (Myrmica rubra) may have brought antimicrobial secretions to the Northeastern United States that are novel weapons against native fungal and bacterial soil organisms. I hypothesized that M. rubra would better inhibit seed pathogens resulting in greater emergence of native myrmecochorous Viola sororia seeds and, as a side effect, more strongly inhibit arbuscular mycorrhizal fungi than a native seed dispersing ant (Aphaenogaster picea …

Nitrogen Availability In Dune Systems And Its Effect On Root Fungal Endophyte Communities., Haley E. Sage

College of Arts & Sciences Senior Theses

As global change persists, changes in resource availability can influence plant-microbe interactions. To understand how resource availability can influence these interactions and species diversity, I focused this research on how varying nitrogen (N) levels affect root endophyte communities in the plant species Ammophila breviligulata, an ecosystem engineer in the dune system. I analyzed the relationship between nitrogen addition and microbial community composition across 60 plots treated with three nitrogen addition levels (control, low, and high) in a long-term experimental field site in the Lake Michigan dunes. I identified Ammophila breviligulata's root endophyte community after creating a culture collection and …

Functional Characterization Of A Putative Alternative Oxidase In Sporisorium Reilianum F. Sp. Zeae., Emma A Lamb

College of Arts & Sciences Senior Theses

Sporisorium reilianum is a pathogenic basidiomycete fungus with two formae speciales, each capable of infecting corn (SRZ) or sorghum (SRS), respectively. This fungus is also a dimorphic variety, meaning it can switch between its haploid, yeast-like sporidia and diploid teliospore stages over the course of its life cycle (Schirawski). When S. reilianum is found in a haploid state and conditions are favorable, it will mate with a compatible non-self mating type to begin filamentous growth and proliferation in the plant host (Zhao). S. relianum, like most fungi, utilizes the four classical components of the electron transport chain to produce …

Microbial Diversity And Community Structure In Sediments Associated With The Seagrass (Thallassia Testudinum) In Apalachicola Bay, Florida, Rahma Ahmed, Thomas Mcelroy, Troy Mutchler

Symposium of Student Scholars

Seagrass is an angiosperm which provides many ecosystem services in coastal areas, such as providing food, shelter and nurseries for many species, and decreasing the impact of waves on shorelines. A global assessment reported that 29% of known seagrass meadows are in a state of decline due to the effects of human activity. Seagrass is commonly found in shallow marine waters where they form meadows containing a microbiome that plays an important role in providing nutrients for seagrass growth, though little is known about the microorganisms within the seagrass meadow sediments. Our project collected sediments from seagrass meadows and adjacent …

Pengaruh Rizobakteri Indigenous Terhadap Serangan Penyakit Budok, Enzim Peroksidase, Dan Pertumbuhan Setek Nilam Aceh, Halimursyadah, Siti Hafsah, Siti Nurminah Nasution, Zuraida

Jurnal Kultivasi

Rizobakteri adalah kelompok mikroba yang aktif dan agressif mengkolonisasi area rizosfir. Mikroba ini mampu berperan sebagai biofertilizer, biostimulan, dan bioprotektan. Tujuan penelitian ini adalah untuk mengetahui jenis isolat rizobakteri indigenous yang berpotensi sebagai agen biokontrol pada patogen Synchytrium pogostemonis penyebab penyakit budok pada tanaman nilam Aceh (Pogostemon cablin Benth.). Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala dan Kebun Percobaan Sektor Timur Fakultas Pertanian, Darussalam Banda Aceh. Waktu pelaksanaan penelitian dimulai pada bulan Januari sampai dengan Mei 2021. Percobaan ini disusun berdasarkan Rancangan Acak Lengkap dengan 15 perlakuan yang terdiri atas 14 perlakuan …

Pemilihan Teknik Aplikasi Dan Dosis Pupuk Hayati Pelarut Kalium Untuk Meningkatkan Serapan Kalium Dan Pertumbuhan Tanaman Jagung Pada Tanah Inceptisols, Diyan Herdiyantoro, Tualar Simarmata, Mieke Rochimi Setiawati, Nenny Nurlaeny, Benny Joy, Mahfud Arifin, Jajang Sauman Hamdani, Iin Handayani

Jurnal Kultivasi

Salah satu strategi yang diterapkan pada pupuk hayati untuk menunjukkan efek positif pada tanaman yang diinokulasi adalah pemilihan teknik aplikasi dan dosis yang tepat, baik pada tanah, benih, atau kombinasi keduanya. Tujuan dari percobaan ini adalah mendapatkan teknik aplikasi dan dosis pupuk hayati pelarut K yang memberikan hasil terbaik terhadap penyerapan K dan pertumbuhan tanaman jagung (Zea mays L.) pada Inceptisols Jatinangor. Percobaan dilaksanakan pada November 2018-Januari 2019 di rumah kaca Fakultas Pertanian Universitas Padjadjaran. Percobaan menggunakan rancangan acak kelompok dengan 9 perlakuan dan 3 kali ulangan. Perlakuan terdiri dari tanpa perlakuan (kontrol), aplikasi pada benih 400 g.ha-1 dan 800 …

Viability Test Of Halotolerant Nitrogen-Fixing Rhizobacteria On Different Carrier Composition And Application Dosage Of Nitrogen Biofertilizer To Increase Rice Growth On Saline Ecosystems, Fiqriah Hanum Khumairah, Fachruddin Azwari, Mieke Rochimi Setiawati, Betty Natalie Fitriatin, Tualar Simarmata

Jurnal Kultivasi

The use of saline soils as productive agricultural land poses major challenges. The utilization of nitrogen biofertilizer with halotolerant N-fixing rhizobacteria as the active material at the right dosage can increase soil productivity and support plant growth. The aim of this study was to obtain the composition of the carrier material that can maintain rhizobacteria viability, water content, and pH of nitrogen biofertilizer and to obtain the right dosage to increase the growth of rice plants in saline ecosystems. The research location was at Microbiology Laboratory of CV Bintang Asri Arthauly, Bandung and greenhouse of Jayamukti Village, Banyusari District, Karawang …

Life Line Spring 2022, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

In this Issue:

A CURE for the Typical STEM Classroom; Attracting Cardinals; Svitana Provides Training to IHS; The Cardinal Science Scholar Program; Mornings with Manatees; Viewing the Department from a New Perspective; Science Lecture Series Features Dr. Tyrone Hayes; Internship with Beinhower Brothers Drilling Company; New Student Chapter: The Botanical Society of America; STEM Art Show 2021; Got Bones?; The Robert and Ann Harting Endowed Award; Grossology Returns; Class Flocks to National Aviary

Effects Of Neem Aqueous Extract (Azadirachta Indica) Against Aphids And Aphid-Borne Virus In Cowpea (Vigna Unguiculata L. Walp), Kehinde T. Kareem, Rachael B. Olayinka, Juliana A. Ugwu, Olubusola F. Oduwaye

Tanzania Journal of Science

Pests and diseases are among the major factors limiting plant growth and yields. Pests are known vectors of pathogens including viruses. Proper management of plant pests is an indirect means of controlling viral diseases in plants. This study aimed at comparing the potential of neem extract and a synthetic insecticide (lambda cyhalothrin) for the management of aphids and Cowpea aphid-borne mosaic virus (CABMV) in five different cowpea (Vigna unguiculata) genotypes and to determine the residual effects of the insecticide in the cowpea grains. The experimental field was laid out in randomized complete block design with three replicates. Treatments included; spraying …

A Rulebook For Peptide Control Of Legume–Microbe Endosymbioses, Sonali Roy, Lena Maria Müller

Agricultural and Environmental Sciences Faculty Research

Plants engage in mutually beneficial relationships with microbes, such as arbuscular mycorrhizal fungi or nitrogen-fixing rhizobia, for optimized nutrient acquisition. In return, the microbial symbionts receive photosynthetic carbon from the plant. Both symbioses are regulated by the plant nutrient status, indicating the existence of signaling pathways that allow the host to fine-tune its interactions with the beneficial microbes depending on its nutrient requirements. Peptide hormones coordinate a plethora of developmental and physiological processes and, recently, various peptide families have gained special attention as systemic and local regulators of plant–microbe interactions and nutrient homeostasis. In this review, we identify five 'rules' …

Overcoming Detrimental Potato Fungi In Potato Fields With The Secondary Metabolites Of Naturally Occurring Steptomyces, Hansan Jones, Jacob Anderson, Erik Kemp, Brad Geary, Matthew Madsen, Ryan Stewart

Library/Life Sciences Undergraduate Poster Competition 2022

• Fungal disease Silver scurf can cause 13% water loss and give poor aesthetic appeal, both of which reduce farmers profits (1,2).
• Pythium leak can result in up to 50% total loss of potatoes in storage (3)
• Streptomyces is the right candidate to mitigate or eliminate these losses because of its ability to survive in soil (4) and its history as a proven biocontrol for other plant pathogens (5).

Mapk-Mediated Transcription Factor Gatad Contributes To Cry1ac Resistance In Diamondback Moth By Reducing Pxmalp Expression, Le Guo, Zhouqiang Cheng, Jianying Qin, Dan Sun, Shaoli Wang, Qingjun Wu, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Zhaojiang Guo, Youjun Zhang

Entomology Faculty Publications

The benefits of biopesticides and transgenic crops based on the insecticidal Cry-toxins from Bacillus thuringiensis (Bt) are considerably threatened by insect resistance evolution, thus, deciphering the molecular mechanisms underlying insect resistance to Bt products is of great significance to their sustainable utilization. Previously, we have demonstrated that the down-regulation of PxmALP in a strain of Plutella xylostella (L.) highly resistant to the Bt Cry1Ac toxin was due to a hormone-activated MAPK signaling pathway and contributed to the resistance phenotype. However, the underlying transcriptional regulatory mechanism remains enigmatic. Here, we report that the PxGATAd transcription factor (TF) is responsible for the …

The Quest To Identify A New Virus Disease Of Sunflower From Nebraska, Robert M. Harveson, Maher Al Rwahnih, California Department Of Food And Agriculture, Alex Karasev, Tom J. Gulya, Jeff Bradshaw

Panhandle Research and Extension Center

Between 2010 and 2018, sunflower plants exhibiting virus-like symptoms, including stunting, mottling, and chlorotic ringspots on leaves, were observed from commercial fields and research plots from four sites within three distinct counties of western Nebraska (Box Butte, Kimball, and Scotts Bluff). Near identical symptoms from field samples were reproduced on seedlings mechanically in the greenhouse on multiple occasions, confirming the presence of a sap-transmissible virus from each site. Symptomatic greenhouse-inoculated plants from the 2010 and 2011 Box Butte samples tested negative for sunflower mosaic virus (SuMV), sunflower chlorotic mottle virus (SuCMoV), and all potyviruses in general by ELISA and RT-PCR. …

Degradation And Nitrogen Cycling In The Context Of Biodiversity-Ecosystem Function Relationships In The Inquiline Bacterial Community Of Darlingtonia Californica, Megan Teigen

Cal Poly Humboldt theses and projects

Biodiversity-ecosystem function (BEF) research aims to explain how species and their environments interact with each other. Microbial communities engage in vital biogeochemical pathways in a variety of natural ecosystems, and yet there are large knowledge gaps about the specific metabolic pathways in which they are involved. Degradation specifically contributes to nitrogen cycling globally through the breakdown of large organic nitrogen compounds into small inorganic nitrogen that is necessary for the survival of many other organisms. In this study, I focused on the degradative function of the inquiline microbial communities found within the carnivorous pitcher plant, Darlingtonia californica. Darlingtonia grows in …

E. Coli Persister Cell Survival And Rhizobia Attachment To Soybean Roots, Tanim Islam

Electronic Theses and Dissertations

The theme of this thesis revolves around how bacteria respond and thrive during stress. Chapters 1-3 are about how bacteria deal with life-threatening antibiotics. Chapter 4 covers new research on how bacteria can move from a stressful individual lifestyle (free-living bacteria) to initiating a symbiotic relationship with a plant (a less stressful lifestyle). In Chapter 1, I briefly summarize the current state of knowledge in the field of antibiotic resistance and persistence. In Chapter 2, I add to this knowledge by providing new insights into several antibiotics' potency and exploring the antibiotic Eagle effect. In Chapter 3, I use pyruvate …

Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi

Electronic Theses and Dissertations

Globally, canola is the second largest oilseed crop after soybean, which is processed for the commercial production of high value oil. The industrial processing steps for canola oil extraction include preprocessing (cleaning, pressing, flaking, and cooking), mechanical pressing, and/or organic solvents. This process results in large quantities of protein-rich (~ 40% dry basis) meal as a co-product. The meal is used partially in animal diet (<30% inclusion) due to high levels of antinutritional factors (ANFs) such as high fibers, phytic acid, and glucosinolates, and low metabolizable energy. Thus, this research was designed to examine various bioprocessing technologies (i.e., traditional sprouting, solid state fermentation, submerged state fermentation, co-culture fermentation, mild pretreatments, and their combinations) for possible advancement in canola use. Canola seed sprouting for 6-day period led to an increase in protein content and a reduction in ANFs and oil content of sprouts compared to ungerminated seed. Subsequent submerged state fermentation of 6- day old sprouts using three different strains (Aureobasidium pullulans, Trichoderma reesei and Neurospora crassa) further increased protein content and lowered ANFs. Solid-state fermentation of 144 h old sprouts using A. pullulans, N. crassa and T. reesei enhanced the protein content and reduced ANFs of sprouts. Sprouting canola seed for three days helped in hull removal, leading to high protein meal accompanied by low fiber and phytic acid level. Three-day seed sprouting had no effect on oil yield, but free fatty acid content was higher compared to seed oil. Co-culture fermentation of HECM under solid state process showed maximum reduction in fiber content with co-culture of A. pullulans and N. crassa while a combination of A. pullulans and T. reesei promoted the highest GLS and phytic acid reductions compared to other combinations, which indicated the advantage of coculture inoculation over monoculture in terms of ANFs reduction. Mild pretreatment of HECM using deionized water resulted in washed HECM (WHECM) with lower soluble sugars and GLS compared to untreated HECM, whereas protein and amino acid were concentrated due to the removal of soluble components. WHECM compared to HECM showed higher protein digestibility when fed to rainbow trout. Subsequent mono- and coculture fermentation of HECM and WHECM under submerged process resulted in higher protein and amino acid content and lower ANFs levels compared to uninoculated controls. The results of cellulase, endoglucanase and β-glucosidase activity indicated the crucial role of used substrates, fungi, fermentation modes (solid state/submerged stated) and inoculation methods (mono-/co-culture) on enzyme activities.

Footprints On The Prairie: Examining The Interlocking Land Histories Of The Liberty Prairie Reserve, Illinois, Anna M. Burns

Scripps Senior Theses

This thesis begins with the local history of the Liberty Prairie, the land where I conducted the ecological field-work that I later discuss in my second thesis on soil microbiome diversity (“Examining Soil Microbial Diversity in Transition Zones Between Corn Fields and Restored Prairie in the Upper Midwest"). I examine the Indigenous histories of the land, and the conflicts between the Bodwéwadmi and Euro-American settlers that resulted in the land being farmed for cattle, corn, and soy for over a hundred and fifty years. I then take a step back and analyze the broader historical contexts of Midwestern Corn Belt …

Examining Soil Microbial Diversity In Transition Zones Between Corn Fields And Restored Prairie In The Upper Midwest, Anna M. Burns

Scripps Senior Theses

Prairies were once the largest ecosystem in North America, but agriculture and settlement has destroyed up to 99% of their pre-colonization extent. Prairie restorations are a strategy to recover the biodiversity and carbon sequestration functions of these grasslands, but typically occur in isolated strips between agricultural fields. My thesis analyzes how effective prairie restorations in the Liberty Prairie (northeastern Illinois) are at recovering the diversity of the prairie soil microbiome, focusing on verrucomicrobia abundance, alpha diversity, and soil physical characteristics.

Identification And Characterization Of Cassava Mosaic Begomoviruses In Non-Crop Plants From Unguja And Pemba Islands, Joseph A. Mwakosya, Gladness E. Temu, Joseph C. Ndunguru

Tanzania Journal of Science

Cassava mosaic disease caused by cassava mosaic begomoviruses (CMBs) remains one of themajor constraints to cassava production since it causes yield losses up to 100%. Researchesshowed that some of CMBs infecting cassava plants may be coming from non-crop plants. Thisstudy intended to identify and characterize CMBs isolated from non-crop plant species inUnguja and Pemba Islands. A total of 108 viral symptomatic and asymptomatic non-crop leafsamples were collected using stratified sampling. DNA was extracted by using CTAB protocolfollowed by polymerase chain reaction using universal and specific primers for cassava mosaicbegomoviruses identification. Results showed that, among eleven samples that were amplified,four were …

Serapan Nitrogen Dan Fosfor Serta Hasil Jagung Yang Dipengaruhi Oleh Aplikasi Pupuk Hayati Dengan Berbagai Teknik Dan Dosis Pada Tanah Inceptisols, Betty Natalie Fitriatin, Muhammad Iqbal Maulana Yusuf, Anne Nurbaity, Nadia Nuraniya Kamaluddin, Meddy Rachmady, Emma Trinurani Sofyan

Jurnal Kultivasi

Upaya meningkatkan unsur hara nitrogen (N) dan fosfor (P) tanah secara berkelanjutan adalah dengan memanfaatkan agen hayati bakteri penambat nitrogen dan bakteri pelarut fosfat. Percobaan pot dilakukan dengan tujuan untuk menentukan teknik aplikasi pupuk hayati dalam meningkatkan kandungan dan serapan hara N dan P serta hasil tanaman jagung pada Inceptisols Jatinangor. Percobaan dilaksanakan di Lahan Percobaan milik Laboratorium Kesuburan Tanah dan Nutrisi Tanaman, Fakultas Pertanian Universitas Padjadjaran. Pupuk hayati yang digunakan merupakan konsorsium bakteri penambat N (Azotobacter chroococcum dan Azospirillum sp.) dan bakteri pelarut P (Pseudomonas malei dan Bacillus subtillis). Percobaan menggunakan Rancangan Acak Kelompok (RAK) dengan sembilan perlakuan dan …

Genetic Characterization Of Antimicrobial Activities Of Endophytic Bacteria Burkholderia Strains Ms455 And Ms389, Jiayuan Jia

Theses and Dissertations

Strains MS455 and MS389, endophytic bacteria, were isolated from healthy soybean plant growing adjacent to a patch of plants affected by charcoal rot disease, caused by the fungal pathogen Macrophomina phaseolina. The complete genomes of both strains were sequenced and identified as Burkholderia species Strain MS455 exhibits broad-spectrum antifungal activities against economically important pathogens, including Aspergillus flavus. Random and site-specific mutations were employed in discovery of the genes that share high homology to the ocf gene cluster of Burkholderia contaminans strain MS14, which is responsible for production of the antifungal compound occidiofungin. RNA-seq analysis demonstrated ORF1, a …

Complete Genome Sequence Of The Pantoea Phage Ah07, Gregory P. Krukonis, Sam J. Roth, Veronique A. Delesalle

Biology Faculty Publications

Bacteriophages of the phyllosphere have not been extensively described, despite their role in bacterial communities on this plant organ. Here, we describe a temperate Pantoea phage, AH07, that was isolated from the leaves of horse chestnut trees. The 37,859-bp linear double-stranded DNA genome contains 58 putative genes, including an integration cassette.

Complete Genome Sequences Of Four Phages Of The Horse Chestnut Phyllosphere, Gregory P. Krukonis, Sam J. Roth, Veronique A. Delesalle

Biology Faculty Publications

Bacteriophages play important roles in determining bacterial communities, including plant microbiota. Here, we describe four lytic phages, three Siphoviridae and one Podoviridae, isolated from four different bacterial species found on the leaves of horse chestnut trees. Their double-stranded DNA (dsDNA) genomes range from 39,095 to 46,062 bp and contain 51 to 70 genes.

Genome Sequences Of Erwinia Phyllophages Ah04 And Ah06, Gregory P. Krukonis, Sam J. Roth, Veronique A. Delesalle

Biology Faculty Publications

Although crucial in shaping bacterial communities, few bacteriophages of the phyllosphere have been described. We provide genome data for two Myoviridae phages, AH04 and AH06, isolated on Erwinia billingiae strains. AH04 shares limited genetic similarity with previously described phages, while AH06 shares over 75% similarity with various Erwinia phages.

The Regulation Landscape Of Mapk Signaling Cascade For Thwarting Bacillus Thuringiensis Infection In An Insect Host, Zhaojiang Guo, Shi Kang, Qingjun Wu, Shaoli Wang, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Youjun Zhang

Entomology Faculty Publications

Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as central hubs of these complex interactions. We have previously shown that an insect hormone modulated MAPK signaling cascade participates as a general switch to trans-regulate differential expression of diverse midgut genes in the diamondback moth, Plutella xylostella (L.) to cope with the insecticidal action of Cry1Ac toxin, produced by the entomopathogenic bacterium Bacillus thuringiensis (Bt). The relationship between topology and functions of this four-tiered phosphorylation signaling cascade, however, is an uncharted territory. Here, we carried out a genome-wide characterization of all the MAPK orthologs …

Respons Pertumbuhan Pakcoy Terhadap Asam Humat Dan Trichoderma Dalam Media Tanam Pelepah Kelapa Sawit, Ratih Rahhutami, Aline Sisi Handini, Dwi Astutik

Jurnal Kultivasi

Pemanfaatan limbah organik dari perkebunan sebagai media tanam pakcoy (Brassica chinensis L.) diharapkan dapat ditingkatkan dengan penggunaan pupuk organik serta pupuk hayati. Penelitian disusun menggunakan Rancangan Acak Kelompok (RAK) pola faktorial. Faktor pertama adalah dosis asam humat meliputi 1, 3, dan 5 g. Faktor kedua adalah dosis Trichoderma sp., meliputi 50, 100, dan 150 mL. Data yang diperoleh kemudian dianalisis ragam pada taraf nyata 5%. apabila terdapat pengaruh nyata, dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa aplikasi asam humat dan Trichoderma sp. memiliki pengaruh mandiri dan tidak terdapat interaksi. Dosis asam humat 3 g per tanaman menghasilkan jumlah daun, …