Microbiology Commons^™

The Voluntary Water Quality Monitoring Program : 1982 Project Report, Barbara Welch, Judy Potvin, Mary Ellen Dennis

Maine Collection

The Voluntary Water Quality Monitoring Program : 1982 Project Report

by Barbara Welch, Judy Potvin & Mary Ellen Dennis

Division of Environmental Evaluation and Lake Studies, Maine Department of Environmental Protection, Augusta, Maine 1982.

Contents: Understanding Maine's Lakes and Ponds / Acknowledgement / Temperature / Dissolved Oxygen / Other Measures of Lake Water Quality / Nutrients / Algae and Chlorophyll a / Transparency / Morphometry / Sampling / Protection / Classification / Answers to Most Frequently Asked Questions / Glossary of Terms / Appendix A : Maximum, Minimum, and Mean Values / Appendix B : Phosphate Content of Common Cleansers …

A Genetic And Physiological Study Of An Arsenite Resistant, Uncoupled Mutant Of Escherichia Coli, Daniel Gordon Smiley

Dissertations and Theses

Chromosomally determined arsenate resistance in Escherichia coli is well characterized. Little, however, is known about chromosomally determined arsenite resistance in E. coli. Accordingly, spontaneous arsenite resistant mutants were selected in a plasmid free strain of E. coli. One mutant strain was analyzed in detail, genetically and physiologically. The mutation confering arsenite resistance was shown to be a single gene mutation. Genetic mapping studies using conjugation and transduction showed that the mutation was closely linked to the ilv region of the E. coli map.

Physiological studies of this mutant showed that it shared characteristics common to those of mutants …

Characterization Of Gram-Positive Bacterial Isolates From Burn Victims, Trisha A. Hannan

Dissertations and Theses

Streptococcus and Staphylococcus were collected from patients hospitalized at the Oregon Burn Center, Emanuel Hospital, Portland, Oregon, between October 27, 1980 and January 1, 1981. Forty-five isolates of Staphylococcus and twenty-one isolates of Streptococcus, Lancefield Group D, were included in the investigation.

The objectives of the investigation were: (i) to identify to the species level all isolates, (ii) to determine their susceptibility to antimicrobial agents, (iii) to test the organisms. over time for increasing resistance to silver, (iv) to determine, if present, a bacterium harbored within the burn unit (nosocomial bacterium), and (v) to determine any recognizable pattern of successive …

Fungal Metabolite From Members Of The Genus Rhizopus, D Thompson, A Smalley, Broderick Eribo

Department of Biology Faculty Publications

A fluorescent metabolite present in seven members of the genus Rhizopus was isolated. This compound appeared green before spray treatment and purple after spray treatment with p-anisaldehyde in visible light. Subsequent purification and structural elucidation of the isolated compound yielded 1-[2,6,10,14-tetramethyl-17-carbomethyl heptadecyl]-1-[2,6,10,14-tetramethyl-17-methanoyl heptadecyl]-benzene.

Interpopulation Variation In Calcareous And Proteinaceous Shell Components In The Stream Limpet, Ferrissia Rivularis, Carl M. Way, Daniel J. Hornbach, Albert J. Burky

Biology Faculty Publications

Natural populations of the North American stream limpet, F. rivularis, were studied in upstate New York [USA], in a set of localities whose waters have a 15-fold range of dissolved Ca (4.6-67.6 mg/liter) and also range from oligotrophy to eutrophy. Shell component analyses (CaCO3, total organic C and total N) are reported as component mass-fractions (mg/g or .mu.g/g dry weight) and as values for a standard limpet shell of 35 mm aperture length (AL). More than 2-fold differences occur between populations in all 3 components, with relatively little variation occurring within each population. Expressed per standard limpet, CaCO3 values for …

Seasonal Metabolism Of The Sphaeriid Clam, Musculium Partumeium (Say), From A Permanent And A Temporary Pond, Carl M. Way, Daniel J. Hornbach, Albert J. Burky

Biology Faculty Publications

Seasonal metabolic rates were examined for the sphaeriid clam, Musculium partumeium (Say) from a permanent and a temporary pond. For the fall-born generation from the permanent pond and for the single generation from the temporary pond, metabolic rates peak during periods of greatest growth and reproduction. Metabolic rates were consistently higher for the permanent pond population. These rates for Musculium partumeium extend the available information on the family Sphaeriidae to the genus Musculium.

Growth Of Pisidium Casertanum (Poli) In West Central Ohio, Albert J. Burky, Daniel J. Hornbach, Carl M. Way

Biology Faculty Publications

Clarke (1973) states that Pisidium casertanum (Poli) is perhaps the most common species of Pisidium and is truly cosmopolitan, occurring throughout Eurasia, Africa, Australia, and South, Central and North America. Populations of these clams are reported in habitats ranging from ephemeral ponds to benthic zones of deep lakes. Our study describes Manuscript received 29 May 1979 and in revised form 25 July 1980 (#79-34). 2 Present address: Department of Biology, University of Virginia, Charlottesville, VA 22901. 3 Present address: Department of Zoology, Miami University, Oxford, OH 45056. 41 aspects of the life-cycle of P. casertanum in a spring-fed stream with …

Plasmid-Mediated Resistance To Arsenite And Arsenate In Escherichia Coli, Thomas Patrick Trezona

Dissertations and Theses

Bacterial strains resistant to arsenite, arsenate (or both) are readily isolated from nature. The resistance, in many cases, can be shown to be determined by conjugal plasmids. The mechanisms by which these plasmids confer resistance to arsenic compounds are entirely unknown. This study was undertaken to shed light on some of the physiological and genetic aspects of this resistance using plasmid-bearing strains of Escherichia coli which are highly resistant to both arsenite and arsenate. Most studies were carried out using E. coli J53 (R773) or the plasmid-free strain J53.

Macroinvertebrates And Reservoir Discharge: Effects Of The Cave Run Lake On Tailwater Communities, Stephen James Jordan

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Stephen James Jordan on May 2, 1980.

A Limnological Study Of 43 Selected Maine Lakes, Derrill J. Cowing, Matthew Scott

Maine Collection

A Limnological Study of 43 Selected Maine Lakes

by Derrill J. Cowing and Matthew Scott

U.S. Geological Survey : Water-Resources Investigations 80-69

Prepared in cooperation with the Maine Department of Environmental Protection

U.S. Geological Survey, 26 Ganneston Drive, August, Maine 04330

Occurrence And Characteristics Of Nonfruiting Myxobacteria Isolated From Pine Forest Soils, Paul M. Kulvi

All Master's Theses

A study was conducted to contribute to the taxonomy of the nonfruiting myxobacteria and to determine the effects of fire on the occurrence and characteristics of nonfruiting myxobacteria isolated from pine forest soil Three years after a prescribed burn, the population of nonfruiting myxobacteria was decreased in the burn site. The organisms isolated were distinct from any of the known species of nonfruiting myxobacteria, and apparently are new species.

Culture And Growth Of Closterium Ehrenbergii (Desmidiaceae), David Bruce Renstrom

Dissertations and Theses

Methods were devised for the isolation and pure culture in defined mineral medium of Closteriurn ehrenbergii Menegh (Chlorophyceae). The effect of pH, type of nitrogen source, nitrate concentration, total hardness, and various buffers on growth were investigated. Best growth rates were obtained at pH 8 to 9, but good growth was observed even at pH 9.4. Nitrate, ammonia, or urea nitrogen could be utilized, but at concentrations greater than 10^-2M urea was toxic to growth. At higher concentrations of ammonia and nitrate longer lag phases of growth were obtained but eventual higher cell yields were obtained. Good growth …

The Biology Of Coprinus Radians, Ozonium, Oidium, And Their Interrelationships, Thomas J. Fahey

All-Inclusive List of Electronic Theses and Dissertations

The pleomorphism of Coprinus radians was studied. The three distinct growth forms--sporophore, oidial stage, and ozonium stage--were examined in relation to ultrastructure, morphology and development. Scanning and transmission electron microscopy are correlated with photomicrographs of developmental stages. Development of the oidia and of the sporophore are of particular interest. The multinucleate oidia develop quite differently from oidia as generally described for Basidiomycete imperfect stages. Development of the oidia of this Coprinus is holoblastic and sympodial. The mushroom primordia are differentiated internally into gill, cap, and stipe hyphae while only l-2mm in diameter. At this stage they look superficially like sclerotial …

Buoyancy Changes As Related To Respiratory Behavior In An Amphibious Snail, Pomacea Urceus (Müller), From Venezuela, Kathleen A. Burky, Albert J. Burky

Biology Faculty Publications

The ampullariid Pomacea urceus uses its ctenidium and lung in respiration. The snails reach the surface with their siphon and ventilate their lung by withdrawing their head-foot in a pumping action. The lung gas also serves to change the buoyancy of the snails. After a ventilation a snail does not necessarily return to the same buoyancy level. They may achieve overall specific gravities of <1 to > 1 after a ventilation. They may remain submerged (s.g. > 1) or float (s.g.

The Isolation And Identification Of Yeast-Like Fungi In Non-Active Tuberculosis Patients In Rowan County, Kentucky, Connie Harris Baker

Morehead State Theses and Dissertations

A thesis submitted to Morehead State University in partial fulfillment of the requirements for the degree of Master of Science in Biology by Connie Harris Baker in July of 1976.

Accumulative Coliform Population In A Five-Mile Section Of The Cibolo Creek, Norman Merrill Brown

Theses & Dissertations

A study of the accumulative coliform population in a five-mile section of Cibolo Creek near Schertz, Texas.

The greatest danger associated with water is that it may recently have been contaminated by sewage or by human excrement. Therefore, the protection of public water supplies from intestinal contamination is a necessary obligation of the public health authorities. If there happens to be a break in the protective chain (adequate treatment, disinfection, and protection of water supply), a chance exposure to fecal contamination could trigger an explosive outbreak of disease within a community. Modern technology and more sophisticated bacteriological methods have made …

Seasonal Periodicity Of Dominant, Benthic, Marine Macroalgae Of The North Jetty Of Brazos Santiago Pass, Texas, As Related To Environmental Factors, Gary J. Penn

Theses and Dissertations - UTB/UTPA

The seasonality of marine algae may be influenced by a large number of factors. These factors Include water clarity, water temperature, dissolved oxygen, conductivity, salinity, light Intensity, precipitation, air temperature, water turbulence, and daylength. The purpose of this study was to determine seasonal patterns and the effect of these variables on the algae.

The results Indicate that 24 dominant, benthic, genera of algae have two seasonal populations. A winter-spring population Includes algae in abundance between January and May. A fall population includes algae in abundance during September, October and November, Results of environmental studies indicate day-length dominant factors in algal …

A Diurnal Zooplankton Migration Study In Lake Mead, Robert D. Staker

Publications (WR)

The diurnal vertical movement of zooplankton was first recorded in freshwater lakes by Weismann (1877) in Lake Constance (Bodensee), although Cuvier was credited with observing the migration of Daphniae in 1817 (Gushing, 1955). The migration is best observed in deep oligotrophic lakes and migrations of 50 m per day are described (Worthington, 1931). In addition, two species of chaetognaths are reported to migrate 400 m a day in marine waters off of Lisbon (Waterman and Berry, 1967) while Birge (1895) found little evidence of the phenomenon at all in Lake Mendota, Wisconsin. Most zooplankton that migrate rise at night and …

Production Of Iodinated Compounds And The Effect On Strobilation In Aurelia Aurita, William J. Welch

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by William J. Welch on May 2, 1973.

A Limnological Study Of Shadow Lake, A Subalpine Lake At Mount Rainer National Park, Washington, Timothy J. Hall

All Master's Theses

This paper presents a general limnological survey of a subalpine lake ecosystem. Physical, chemical and biological parameters were considered. Chemical and physical factors were dominated by the influence of snow-ice cover and high amounts of solar radiation. Abiotic factors result in a small phytoplankton standing crop and limitation of activity by photosynthetic organisms to the short summer season. Zooplankton and benthic communities are simplified in species composition and population size in response to the small primary producer population.

Spatial Distribution & Temporal Occurrence Of Rotifers In The Main Pool & Tailwater Of Barren Lake, Kentucky, David Abel

Biology Graduate Theses

A study of the spatial distribution and temporal occurrence and diversity of rotifers in relation to certain chemicophysical parameters was conducted in Barren Lake, Kentucky, a flood control lake, from January, 1970, through January, 1970. Paired samples were collected from six depths in the main pool area of the reservoir and from an automatic plankton sampler installed in the tailwater area where, from April through September, the effluent was derived from the upper 3 m of the pool . Diel studies were conducted in April, July, 1970, and January, 1971.

Barren Lake began to stratify in late April, was completely …

The Effect Of Dimethylsulfoxide On The Infectivity Of The T1-Coliphage, Grergory Kyle Reeder

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Gregory Kyle Reeder on July 22, 1971.

Characteristics Of A Bacteriophage Isolated For Pseudomonas Tabaci, Linda R. Nichols

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Linda R. Nichols on July 19, 1971.

Effects Of Insulin, Sodium And D-Glucose On Amino Acid Absorption In The Intestine Of Rats, Andre-Gerard Craan

Dissertations and Theses

Amino acid absorption across rat intestine in vitro was inhibited or stimulated by insulin depending on the hormone serosal concentration.

The absence of sodium ions from the incubating solution resulted in a significant decrease of L-alanine absorption. However, L-alanine was enhanced in a sodium-free medium by the addition of insulin on the serosal side of the intestine.

A sizable decrease in L-alanine and L-lysine absorption was produced by introduction of D-glucose (0.2 % and 2 %) in the Krebs-Ringer's incubating buffer. Nevertheless, the presence of D-glucose did not prevent the stimulatory effect of insulin on amino acid absorption.

Micronutrients And Biological Patterns In Lake Mead, Hasan K. Qashu, Lorne G. Everett, J. S. Carlson, Bureau Of Reclamation

Publications (WR)

Progressive increases in concentration of dissolved solids in the Colorado River water from Lake Powell to Imperial Dam seem to alter plankton dynamics and biological productivity of the river. Also, changes in biological productivity and micronutrients concentrations occur within the same reservoir. Development of a digital simulation model to predict micronutrients concentrations and biological productivity is necessary for diagnosing changes in plankton population and effluent-carrying capacity of the system. The objectives of the study are: (1) to determine trace metal balance at different locations in Lake Mead, (2) to measure biological productivity and conduct plankton population counts at each sampling …

Description And Seasonal Variation In Incidence Of A New Species Of Myxosporidian Parasite (Class Myxosporidea) Of The Bluegill Sunfish, Lepomis Macrochirus Rafineque, In Kentucky, Stephen Bayes Crider

Masters Theses & Specialist Projects

A new histozoic myxosporidian parasite (Class Mysoxporidea), Myxobolus meglitschi sp. nov., infecting young-of-the-year and yearling bluegill sunfish, Lepomis macrochirus Raf., from Shanty Hollow Lake, Warren County, Kentucky was described. Phenology was investigated from January 20 to December, 1969.

The magnitude of infection varied seasonally in bluegill. Incidence was highest in July and August (43.92) in host populations, declining, but not disappearing from September to December 20, 1969. The infection exhibited a yearly mean incidence of 22.318.

The pattern of distribution of cysts on hosts varied seasonally. During periods of low incidence cysts were confined primarily to a postanal area below …

An Ecotypic Differentiation Of Human And Bovine Fecal Streptococci, With Application To Human Pollution In The Yakima River, Harlen Harvey Johnson

All Master's Theses

It was the purpose of this study to report on the development of a technique for distinguishing human fecal streptococci from bovine fecal streptococci, and to make application of this technique in describing some of the sources of pollution in the Yakima River as contributed by the Wilson Creek drainage system, in the Ellensburg area of Washington.

Some Aspects Of Carbohydrate Metabolism In The Cercariae Of Schistosoma Mansoni Sambon, 1907, A. Eugene Dunham Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

Cercariac of Schistosoma mansoni were studied for glycogen utilization, uptake of glucose from solution, lactic acid production, and respiration.

Using the anthrone method of determination of carbohydrate, the amount of glycogen used by a single cercaria in 4 hours is determined as 43.2 x 10^-4 μg ± 6.6 x 10^-4 μg.

Glucose was not observed to have been used from solution under the conditions of the experiment, even in the presence of trehalose. The determinations were made by the Glucostat method.

After deproteinization and desugarization, aliquots of supernatant from cercarial suspensions were analyzed for lactic acid using p-phenylphenol …

A Balanced Salt Culture Medium For Halteria Grandinella, Yee Wan Mark

All-Inclusive List of Electronic Theses and Dissertations

The animals that the writer worked with liere the unicellular organisms called liaJ,tez:ia grandil.!iilJ.a• They belong to the Pl~lum Protozoa and are placed in the Class Ciliata and the Order Oligotrichia. llftltez'A gDJ}gini•U, like most protozoa, ll ve in waters but they seem to prefer qUiet pools, lakes, and ponds in which aqua i;;!c plants are growing and in which there is much organic matter.

Aquatic Phycomycetes Of Lily Lake, Hugh Macmillan Rooney

Theses and Dissertations

Lily Lake, a subalpine lake in the Wasatch National Forest Tl5, R9E, S31, of the Uinta Mountain Region, Utah, is located about one-half mile west of Trial Lake at an elevation of 10,000 feet. This Lake, which is one of the acidic, cold, lentic bogs characteristic of this area, has been selected for a taxonomic and seasonal distribution study of acquatic phycomycetes. This study is a beginning to our knowledge of the role of the fungi in the hydrobiology of Lily Lake and will supply the first information on record of the water molds of this area. The study identifies …