Laboratory and Basic Science Research Commons^™

Using Northern Pike And Yellow Perch To Attempt A Trophic Cascade, Bryan Sea

Journal of Earth and Life Science

In the midwestern USA, water clarity has decreased in shallow lakes as a result of large populations of Fathead Minnow Pimephales promelas and agriculture that impact the life that depend on shallow habitats, such as waterfowl, fish, and aquatic plants. Northern Pike Esox lucius and Yellow Perch Perca flavescens were stocked in a few lakes in attempt to create a tropic cascade and reduce populations of nuisance Fathead Minnows. Northern Pike were stocked as fry and Yellow Perch were stocked as adults either pre-spawn or post-spawn. Lake managers hypothesized adult Yellow Perch would spawn and the age-0 Perch would feed …

Walleye Sander Vitreus Visceral Fat Accumulation, Cole Daleiden, Seth Renner

Journal of Earth and Life Science

For ectothermic species, proper lipid retention is critical to surviving in regions with limited growing seasons. Visceral fat is the dominant form of this energy storage in fish. Build up and depletion of these lipids occur seasonally as activity levels change throughout winter and spring. Due to this annual pattern, the purpose of this study is to determine a trend in the amount of visceral fat Walleye Sander vitreus accumulate in a time of year when metabolic rates are low and transition into the spawning process has yet to occur. Angling was the method of capture conducted during the months …

The Cardiovascular Response To Acute Hypoxic Conditions In Danio Rerio, Bryce Fetterman

Williams Honors College, Honors Research Projects

There are many fish that can withstand hypoxia levels. One in particular is the zebrafish (Danio rerio). The zebrafish is a small-sized, Cyprinid teleost fish, and has been used in a laboratory setting for many reasons. A few reasons being that they are widely available, easy to care for, and the cost for these fish are very low. There are various reasons why these fish are used as a model. One main reason is because their cardiovascular system is one of the first developing organs. This allows for better resolution when studying the cardiovascular system. The purpose of …

Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara

Williams Honors College, Honors Research Projects

I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …

Spatial And Temporal Variation In The Diet Composition Of Zooplankton In Mission Bay, Bryanna Paulson

Theses

Analyses of quantitative data on zooplankton diets are vital for understanding the drivers of zooplankton abundance within an ecosystem. Such analyses also provide insight into trophic pathways within the lower planktonic food web, which support populations of higher trophic level species. This study used carbon and nitrogen stable isotope ratios of size-fractionated plankton in Mission Bay, San Diego, CA to examine the spatial and temporal variation in zooplankton trophic ecology and determine potential environmental drivers of zooplankton community structure. Carbon stable isotopes reflect primary production sources in an organism’s diet, and nitrogen stable isotope ratios can be used to estimate …

The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli

Master's Theses

The spotted seatrout (Cynoscion nebulosus) is a euryhaline finfish candidate for marine aquaculture in the northern Gulf of Mexico. Aquaculture of this species currently uses salinities of 25 psu or greater making production challenging in areas where high salinity water is not available. This study aimed to document the effects of salinity on zootechnical performance of spotted seatrout embryos, early larvae, and juveniles in recirculating aquaculture systems to assist with the development of protocols for low salinity culture.

Embryos obtained from a volitional spawn at 29 psu were incubated at three salinities (12.5, 18.75, or 25 psu) until …

Animal Experimentation: Working Towards A Paradigm Change, Kathrin Herrmann (Ed.), Kimberley Jayne (Ed.)

eBooks

Animal experimentation has been one of the most controversial areas of animal use, mainly due to the intentional harms inflicted upon animals for the sake of hoped-for benefits in humans. Despite this rationale for continued animal experimentation, shortcomings of this practice have become increasingly more apparent and well-documented. However, these limitations are not yet widely known or appreciated, and there is a danger that they may simply be ignored. The 51 experts who have contributed to Animal Experimentation: Working Towards a Paradigm Change critically review current animal use in science, present new and innovative non-animal approaches to address urgent scientific …

Spatial And Temporal Variability In Post-Larval Yellow Perch Density, Steve Hauschildt

Biology Graduate Theses

Yellow Perch Perca flavescens are important as forage for other species and for sport fishing in many northern lakes. However, estimating post-larval Yellow Perch populations can be difficult because of many environmental factors that cause unexplained variation. The objective of this study was to help reduce unexplained variation by determining a post-larval Yellow Perch population density estimate on Blackduck Lake, MN, provide guidance to help determine the number of trawls required for trawling-based recruitment indices to achieve varying levels of precision, and test for the effects of wind speed and direction on post-larval Yellow Perch spatial variability. This study estimated …

Effects Of Rainbow Trout On Brook Trout Population Size In Tributaries To Lake Superior In Cook County, Minnesota, Craig Tangren

Journal of Earth and Life Science

This study looked at the effects of Rainbow Trout stocking on the size of Brook Trout populations above migratory barriers. Linear mixed effects models were used to determine if there was a relationship between Brook Trout and Rainbow Trout catch rates. Age 1 or older Brook Trout populations were influenced by relative abundance of age 1 or older Rainbow Trout, according to the best supported model based on AIC scores. Population responses varied by stream, ranging along a gradient from high catch rates with a positive relationship to a negative relationship with low relative abundances. Streams with a positive relationship …

Changes In Walleye Fecundity And Egg Size Before, During, And After Rehabilitation Of The Red Lakes Fishery, Benjamin J. Miller, Benjamin D. Erb

Journal of Earth and Life Science

The Red Lakes in northern Minnesota experienced a major Walleye Sander vitreus fishery crash in the late 20th century, but has since recovered to record abundances and supports a major commercial and recreational fishery. Fecundity is an integral aspect of recruitment that can contribute greatly to the understanding of fishery population dynamics. To elucidate the effects of varying Walleye densities and exploitation rates on fecundity and egg size in years 1989 (overexploited fishery), 2004 (recovering fishery), and 2017 (recovered fishery), we analyzed linear models that related fecundity or egg size to morphological features for these three sampling periods. Walleyes were …

Goldeye Hidon Alosoides Calorimetry In Red Lake, Jesse Mendel

Journal of Earth and Life Science

Goldeye Hiodon alosoides are found across North America and are a relatively unstudied group of fish as they have had no economic value in the past. Ecologically speaking, these fish could have importance to the waters that they inhabit, as they are prey to fishes like Walleyes Sander vitreus, Northern Pike Esox lucius, and Muskellunge Esox masquinongy. These fish could potentially be vital to predatory fishes because of the amount of oil found in these fish, creating gram for gram more energy than compared to other consumable substances. The average energy density for all Goldeye was 6.73 …

Examining Black Bullhead Ameiurus Melas Age And Growth In Shallow Minnesota Lakes, Michael S. Collins, Anna M. Medina

Journal of Earth and Life Science

Ageing methods, population age structures, and growth trajectories for Black Bullhead Ameiurus melas were evaluated for six populations from shallow lakes divided between two ecoregions in Minnesota, USA. A total of 693 fish were collected in July and August 2018 and measured for total lengths (TL) and weights (g). We collected ageing structures from 320 of these fish. Crosssections of lapillar otoliths (n=191) and pectoral spine structures (n=75) were used to estimate age by two readers in a blind reading procedure. Fish caught ranged in total lengths from ~40 to 350 mm and weighed between 8 and 380 g. Black …

Smart Sheep Need More Protection, Michael L. Woodruff

Animal Sentience

The target article unequivocally establishes that sheep are far more intelligent and cognitively sophisticated than is generally acknowledged. For this reason, the authors advocate for significantly more stringent regulation of agricultural and research practices when sheep are used. I briefly review the existing US regulations governing the use of sheep in research and discuss the extent to which they are applied to sheep. I then discuss weaknesses in the current regulations, concluding that they should be changed to mandate housing all research animals in environments that accommodate the psychosocial needs of each species.

Adult Walleye Abundance And Subsequent Fry Production In The Tamarac River, Mn, Phillip Oswald

Biology Graduate Theses

Walleye reproductive success is of critical interest for the Red Lakes as this system is maintained entirely by natural reproduction and supports robust commercial and recreational fisheries. The Tamarac River, a major tributary to the Red Lakes, hosts a substantial Walleye spawning migration annually. The river drains the largest peat bog in the lower 48 states and thus experiences low dissolved oxygen at times. It is largely unknown how the potential anoxic bog water affects Walleye reproductive success each year and subsequent year class strength. Fyke nets were set from 2014-2018 to assess the relative abundance of adult Walleye spawning …

Growth Of Pelagic Age-0 Yellow Perch In Relation To Hatch Dates And Water Temperature In North-Central Minnesota Lakes, Ethan Karppinen

Biology Graduate Theses

Exposure to consistent water warming rates and access to abundant populations of edible sized zooplankton affects growth and increases the probability of survival for newly hatched Yellow Perch Perca flavescens. Seven lakes with varying physical characteristics were selected in north-central Minnesota to study abiotic (water temperature) and biotic (zooplankton community and fish growth) progressions during the spring and early summer (April-June). Mean water temperatures from ice-out to 28 June (11.98-14.62 °C) had significant relationships with mean depth (P < 0.01, R? = 0.85), max depth (P < 0.01, R? = 0.73), and total volume (P < 0.01, R2 = 0.54). Hatch periods ranged from 13 to 19 days in 2015 and 13 to 25 days in 2016. Cumulative degree-days (CDD) were used to predict Yellow Perch hatch periods and explain growth. CDD predicted 21-81% of hatch periods in 2015 and 11-50% in 2016. Mean predicted total lengths at 28 June were 19.65-30.41 mm in 2015 and 20.88-28.54 mm in 2016. There was a significant relationship between CDD and predicted total length (R? = 0.62, P < 0.01) to explain differences in Yellow Perch total length while inhabiting the pelagic zone. Observed gape measurements were highly correlated with predicted gapes from the Schael formula (R? = 0.89, P < 0.01) that was used to assess the availability of edible sized zooplankton for age-0 Yellow Perch in each lake. No predator-prey mismatches were found and earlier hatch dates produced age-0 Yellow Perch with greater predicted total lengths in late June on the majority of lakes. This suggests that earlier hatched Yellow perch have an advantage over later hatched Yellow Perch in attaining greater lengths by late June. Our study provides information on a variety of physical and biological progressions in lakes, which may be used for fisheries management practices in the future.

Population Characteristics Of A Closed Northern Pike (Esox Lucius) Catch-And-Release Population In Minnesota, John Kempe

Biology Graduate Theses

Literature regarding catch-and-release populations of Northern Pike are rare and provide a unique perspective on their population dynamics. Shingobee Lake is a 64- ha mesotrophic lake with a maximum depth of 12.2 m located in northern Minnesota. Northern Pike fishing has been catch-and-release only since 2005, and there are no public accesses on the lake. Annual ice-out trap-netting occurred from 2009-2017, and a sizable amount of the Northern Pike recaptured via trapnet or angling had been previously tagged (37-59%). The lack of exploitation and high tagging percentage provided a unique opportunity to study the population dynamics of Northern Pike. Shinogbee’s …

Growth Rate And Hatch Date Of Age-0 Fishes On Leech Lake, Minnesota Before Zebra Mussel Establishment, Kayla Morey, Emily Larson

Journal of Earth and Life Science

The introduction of an invasive species to an ecosystem is considered a very severe environmental problem. Zebra Mussel veligers Dreissena polymorpha have recently been found in Leech Lake, Minnesota, and are likely going to have a large influence on this ecosystem. Zebra Mussels are filter-feeders that rely on phytoplankton and zooplankton as their main source of energy. A shift in populations of plankton due to Zebra Mussels may affect the growth rates of larval fish. Median hatch dates and growth rates were estimated from four age-0 fish species (Yellow Perch Perca flavescens, Walleye Sander vitreus, Smallmouth Bass Micropterus …

Seasonal Fluctuations Of Zooplankton, Dissolved Oxygen, And Temperature In Lake Bemidji, Minnesota, Cody Coyle

Journal of Earth and Life Science

With the current influx of aquatic invasive species in Minnesota lakes, the status of zooplankton has become vital. Zooplankton are at the base of all food chains in aquatic ecosystems, ecosystems that vary greatly throughout a year’s time. The objectives of this study were to 1) track the location of zooplankton throughout the water column for an entire year, and 2) establish relationships between zooplankton location and specific water column characteristics. Zooplankton, dissolved oxygen, and temperature samples were collected bi-monthly at various depths at the deepest point in the southern basin of Lake Bemidji. Zooplankton were most abundant in the …

Age-0 Walleye Growth Rates Following Spiny Water Flea Bythotrephes Longimanus Invasion, Joseph Amundson

Journal of Earth and Life Science

Within Voyageurs National Park, spiny water flea Bythotrephes longimanus has reduced Lake Kabetogama and Rainy Lake native zooplankton biomass during summer peak by 40-60%. Subsequently, planktivorous age-0 Yellow Perch Perca flavescens growth has decreased. A possible trophic cascade may influence predatory fish growth rates like that of age-0 Walleye Sander vitreus. Using seine net and electrofishing data, age-0 Walleye growth rates were modeled as a linear function of growing degree days (GDD) and the slope was compared between pre- and post-spiny water flea establishment. Nearby Lake Vermilion, assumed to have been unaffected by spiny water flea until 2015 and …

Burbot Activity In Relation To Light Penetration On Lake Bemidji, Mn, Hunter Weidenborner, Josh Janecek

Journal of Earth and Life Science

Burbot Lota lota is the only freshwater member of the family Gadidae and has a circumpolar distribution in cold-water regions. Information related to Burbot biology is limited in comparison to most fishes. Snow pack depth and ice conditions influence light penetration and therefore primary production levels. Primary production can have cascading effects on higher trophic levels. Therefore, the objective of this study was to observe if light levels penetrating through cleared and covered ice has an effect on Burbot activity in Lake Bemidji, MN. Angling for Burbot occurred for a minimum of 30 four hour sample periods at both cleared …

Hunter Harvest Effect On The Breeding Bird Population Of Northern Pintail Anas Acuta In North America, Matthew Wang

Journal of Earth and Life Science

Hunter harvest and the effects that it can have on population dynamics is a relationship used by wildlife professionals when making management decisions. Hunter harvest can be compensatory or additive on wildlife populations and instances where hunter harvest has been a key role in a population decline, and sometimes demise, is an occurrence studied in wildlife management. Waterfowl numbers in North America have increased 17% since 1960, but during that same time frame the Northern Pintail population has decreased 40%. Hunter harvest data has been recorded by the U.S. Fish and Wildlife Service since 1961, while they have simultaneously been …

Dopamine Levels In The Brain Of Rat Models Of Human Rheumatoid Arthritis, Amelia Stinson

Theses & Dissertations

Research Focus. Rheumatoid arthritis (RA) is a chronic, debilitating, autoimmune disease that causes the destruction of bone tissue and the articular structures of joints. At least 30% of RA patient populations have cognitive impairment. Acidic dopamine (DA) is the principal neuroimmunotransmitter that links the central nervous system and peripheral nervous system together. The aim of the present study was to determine the levels of DA and its two acidic metabolites: 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in arthritic induced rats, and whether their levels vary across four different parts of the brain: amygdala (AMG), front cerebral cortex (CX), hippocampus …

Estimating Seasonal Variation In Condition Of Yellow Perch, Emily Anne Powers

Biology Graduate Theses

Research has shown that Yellow Perch Perca flavescens, found throughout lakes and streams of North America, are good indicators of environmental health. Due to the correlation between fish condition and ecosystem health it is essential to reliably estimate condition to better manage our fisheries. Therefore, the study objectives were to measure the seasonal changes in adult Yellow Perch percent dry weight (PDW), and compare the reliability of bioelectrical impedance analysis (BIA) and morphometric-based estimates of condition. The seasonal fluctuations in average monthly PDW were measured over a two- year period and were sinusoidal in shape with peaks in August 2015 …

Relationship Between Zooplankton And Walleye Rearing Pond Success, Danny Tuckett

Journal of Earth and Life Science

Walleye Sander vitreus are an important sport fish in Minnesota. This has led to the raising of Walleye in ponds to later be stocked into lakes. This study was done to determine the relationship between Walleye prey (zooplankton) and the success of Walleye in 11 stocked ponds in the Ortonville, MN area. There was no management of the ponds until harvest, which starts in late September and ends in November. There were significant positive relationships between rotifer density (P = 0.03) and nauplii density (P = 0.04) to kg of Walleye harvested. The setting where nauplii and rotifer density are …

Redd Habitat Selection For Brook Trout In The Necktie River Bemidji, Mn, William Varela, Zachary Buckley

Journal of Earth and Life Science

The presence of quality suitable habitat is important for the success of naturally reproducing Brook Trout populations. Therefore, measuring redd habitat Brook Trout select for was the focus of this study. Brook Trout redds were observed during the spawning season of 2016. We identified a total of 18 redds in the Necktie River in late October and early November. Specific environmental variables associated with redd selection were measured: velocity, in-stream cover, canopy cover, bank overhang, river width, substrate type and size, temperature, and max-depth. The same environmental variables were measured for 30 randomly selected sites. Redds were distributed in riffles …

Low Cost, Multi-Purpose Genotyping Panels For Dairy And Beef Cattle, Michelle Judge

Theses

rhe selection of animals based on DNA has revolutionised animal breeding, but the associated high cost of obtaining genotypes has limited its uptake. The objective of this thesis was to develop a low-cost, low-density, multi-purpose genotyping panel for the procurement of reliable genotype information, and to quantify the long-term consequences of using such low-density genotype panels in breeding programs. The objectives were achieved through a combination of real-life cattle genotype data and simulations to mimic a cattle population. The in-silico development of genotype panels was based on actual genotypes from up to 58,705 beef cattle. Alternative novel strategies were used …

Planaria: An Animal Model That Integrates Development, Regeneration And Pharmacology, Oné R. Pagán

Biology Faculty Publications

Although planarians are established model organisms in developmental biology and regeneration studies, in the last forty years or so, they have caught the attention of pharmacologists, especially to study the pharmacology of drugs of abuse. This review covers the following topics: some fundamentals of the history of animal models and planarians in biomedical research; an abbreviated story of systematic pharmacology research using planarians as a model organism; an example of how planarians are contributing to the search for compounds against acute cocaine toxicity; an analysis of the number of papers on planarians and pharmacological topics from 1900- 2016; some perspectives …

Burbot Growth Rates In A Northern Mesotrophic Lake, Samuel J. Peterson

Journal of Earth and Life Science

Burbot Lota lota has a circumpolar distribution throughout cold-water areas and is the only freshwater member of the family Gadidae. There are currently very few management techniques applied to Burbot throughout their northern extent. Growth is an indicator of fish health, biomass, and habitat quality; therefore, the objective of this study was to determine the growth rate of Burbot in Lake Bemidji. Burbot (n = 45) were collected along three sites on Lake Bemidji for years 2015-2017. Age was determined from sagittal otoliths, the marginal zones of which were opaque from December to May and translucent from June to November. …

Effects Of Catch-And-Release Angling On Egg Densities In Largemouth Bass Nests, Tyler Robinson

Journal of Earth and Life Science

Predation during early life stages of Centrarchidae offspring are a major cause of decreased reproductive success. For five-weeks Largemouth Bass Micropterus salmoides defend their broods from invaders such as Yellow Perch Perca flavescens and Bluegill Lepomis macrochirus. The focus of this study was to determine if predation upon Largemouth Bass broods increased as removal time from the nest via catch-and-release angling increased. Sampling was conducted on the Whitefish Chain, MN from 7 May - 7 June 2016, where a total of 29 nests were located and sampled. Three treatment groups were used; immediate catch-and-release, three-minute, and a seven- minute …

Effect Of Abiotic Factors On Bluegill Size Structure In Nine Central Minnesota Lakes, Ryan Henry

Journal of Earth and Life Science

Bluegill are the most targeted species of fish in Minnesota. In recent years, the abundance of slow growing, small Bluegill has increased within the state. Angler harvest of Bluegill can reduce the average length of Bluegill up to four times the original length. Abiotic factors such as maximum depth, average water clarity, littoral area, and percent littoral area can influence the dynamics of a lake system. There has been limited research on whether these abiotic factors can influence the size structure of a Bluegill population. The objective of this study is to determine if there is a direct relationship between …