Laboratory and Basic Science Research Commons^™

Are Weevils Picky Eaters? Community Structure And Host Specificity Of Neotropical Saproxylic Beetles (Coleoptera: Curculionidae), Jhunior A. Morillo

Dissertations and Theses

Abstract Primary saproxylic beetles play a major role in forest nutrient cycling and making deadwood accessible to other decomposers. Understanding beetle host preferences and patterns of community assembly is critical for their conservation, and for predicting which species might become invasive. This project aims to investigate the ecological and host specificity, as well as the community composition of curculionids in a mosaic of old-growth (OG) and secondary forest on the Osa Peninsula, Costa Rica. The subfamily Scolytinae was expected to be the most species-rich and abundant. Ambrosia beetles were expected to have more generalist species than other curculionids. Old growth …

Benthic Algae And Diatom Communities In Seagrass Meadows Under Three Different Human Impact Regimes In Bocas Del Toro, Panamá, Averyl Cheng

Independent Study Project (ISP) Collection

In Bocas del Toro, Panamá, widespread tourism has been the main source of revenue and has become an increasing threat to seagrass meadows and the organisms they support. This study aimed to investigate and describe algae and diatom communities under three different regimes of anthropogenic disturbance: high, medium and low human impact. The biodiversity was analyzed by measuring the algae and diatom assemblages with Shannon-Weiner’s Biodiversity Index, Evenness Index and Sorensen’s coefficient. The data obtained from these three diversity indices were compared to the areas along an anthropogenic disturbance gradient of high, medium and low impact. A total of 12 …

Influencing Pathways That Cause Metastasis And Stemness In Epithelial Ovarian Cancer, Alyse Lynn Huisken-Hill

Electronic Theses, Projects, and Dissertations

Ovarian cancer is the fifth leading cause of cancer death in women between the ages of 35 and 74. With 22 thousand new cases and 15 thousand deaths annually ovarian cancer is among the most deadly cancers with a death to incidence ratio of 68%. With 70% of cases High Grade Serous Ovarian Carcinoma (HGSOC) is the most common type of ovarian cancer and causes 90% of ovarian cancer deaths. 80% of patients have reoccurrence within five years and only 15-30% of patients with recurrent metastatic ovarian cancer respond to current therapies, chemotherapy and surgery. One reason for the high …

Rescuing Perishable Neuroanatomical Information From A Threatened Biodiversity Hotspot: Remote Field Methods For Brain Tissue Preservation Validated By Cytoarchitectonic Analysis, Immunohistochemistry, And X-Ray Microcomputed Tomography, Daniel Hughes, Ellen Walker, Paul Gignac, Anais Martinez, Kenichiro Negishi, Carl Lieb, Eli Greenbaum, Arshad Khan

Selected Works Temporary Series

Biodiversity hotspots, which harbor more endemic species than elsewhere on Earth, areincreasingly threatened. There is a need to accelerate collection efforts in these regionsbefore threatened or endangered species become extinct. The diverse geographical, ecological,genetic, morphological, and behavioral data generated from the on-site collection ofan individual specimen are useful for many scientific purposes. However, traditional methodsfor specimen preparation in the field do not permit researchers to retrieve neuroanatomicaldata, disregarding potentially useful data for increasing our understanding of braindiversity. These data have helped clarify brain evolution, deciphered relationships betweenstructure and function, and revealed constraints and selective pressures that provide contextabout the evolution …

Rescuing Perishable Neuroanatomical Information From A Threatened Biodiversity Hotspot: Remote Field Methods For Brain Tissue Preservation Validated By Cytoarchitectonic Analysis, Immunohistochemistry, And X-Ray Microcomputed Tomography, Hughes, Ellen Walker, Paul Gignac, Anais Martinez, Kenichiro Negishi, Carl Lieb, Eli Greenbaum, Arshad Khan

Departmental Papers (Biology)

Biodiversity hotspots, which harbor more endemic species than elsewhere on Earth, areincreasingly threatened. There is a need to accelerate collection efforts in these regionsbefore threatened or endangered species become extinct. The diverse geographical, ecological,genetic, morphological, and behavioral data generated from the on-site collection ofan individual specimen are useful for many scientific purposes. However, traditional methodsfor specimen preparation in the field do not permit researchers to retrieve neuroanatomicaldata, disregarding potentially useful data for increasing our understanding of braindiversity. These data have helped clarify brain evolution, deciphered relationships betweenstructure and function, and revealed constraints and selective pressures that provide contextabout the evolution …

Assessment Of American White Pelican Predation And Reproductive Success Of Walleye In The Tamarac River, Mn, Jake Graham

Biology Graduate Theses

The Tamarac River is a major tributary of the Red Lakes MN, which provides an economically important multispecies fishery. The river experiences spring spawning migrations of northern pike, walleye, and white suckers. However, limited data exists related to reproductive success of fishes in the river. Furthermore, in the previous decade large congregations of American white pelicans (AWPE) have been observed using the Tamarac River as a foraging ground each spring. Assessing reproductive success of fishes in the Tamarac River provides a more holistic understanding of fish ecology in the river and the importance of the Tamarac River to the Red …

Survival Of Walleye Eggs In Upper Red Lake And The Tamarac River, Phillip Oswald

Journal of Earth and Life Science

Walleyes experience spawning runs each spring in the Tamarac River, a tributary of the Red Lakes. However, data about walleye egg survival in the lake and river is lacking. Mesh cages were placed in both the Tamarac River and Upper Red Lake to compare survival rates. A total of twenty cages were set, ten in the river and ten in the lake. Each cage contained one hundred fertilized eggs which were checked every third day to measure survival. Temperature (°C), dissolved oxygen (mg/L), and pH were measured at each cage location. The river had an average survival rate of 22% …

An Analysis Of The Interaction Of Methylphenidate And Nicotine In Adolescent Rats: Effects On Bdnf, Elizabeth D. Freeman

Electronic Theses and Dissertations

This investigation was an analysis of the interaction of adolescent exposure to methylphenidate (MPH; trade name: Ritalin) on nicotine sensitization and conditioned place preference (CPP) in a rodent model and underlying mechanisms of this effect. Animals were treated IP with 1 mg/kg MPH or saline using a ―school day‖ regimen of five days on, two days off, from postnatal day (P) 28-50. During the final two weeks of MPH treatment, animals were either behaviorally sensitized to nicotine (0.5 mg/kg free base) or saline for 10 days, or conditioned to nicotine or saline using the CPP behavioral paradigm. In addition, three …

The Development Of Skeletal Muscle In Young Horses: An Ultrasonography And Satellite Cell Approach, Emma K. Lavigne

Honors Scholar Theses

Muscle growth in young horses is characterized by an increase in muscle cross-sectional area, which can be accomplished through the activation and differentiation of satellite cells. Satellite cells can be stimulated or inhibited in response to different cytokines and growth factors and are key mediators of muscle hypertrophy and regeneration. The aim of this study was to investigate the growth of the longissimus dorsi (LD) muscle in horses under 5 years of age and to obtain preliminary data on satellite cell behavior in foals. The area, width, height, and subcutaneous fat were measured using ultrasonography at 6-month increments over the …

Effects Of Pre-Release Physical Stressors On Post-Release Success Of Hatchery-Reared Spotted Seatrout, Taylor Westbrook Guest

Master's Theses

Alteration of habitat associated with coastal development and increased demand for food and recreation can result in the depletion of fisheries resources such as the Spotted Seatrout (Cynoscion nebulosus), the Gulf of Mexico’s most popular recreational fish. Stock enhancement, or the release of cultured fish to supplement wild populations, is one potential tool for managing important fisheries resources. Although much effort has gone into marine stock enhancement, the effectiveness of stocking is not well established, and techniques for ensuring success have not been developed. Although the basic biology of the Spotted Seatrout is well known, comparatively little is known about …

Developing A Catalog Of Socio-Sexual Behaviors Of Beluga Whales ( Delphinapterus Leucas ) In The Care Of Humans, Heather M. Hill, Sarah Dietrich, Deirdre Yeater, Mariyah Mckinnon, Malin Miller, Steve Aibel, Al Dove

Psychology Faculty Publications

The repertoire of socio-sexual and sexual behaviors of cetaceans is relatively unknown. The purpose of the current study was to advance the existing knowledge of socio-sexual behavior of beluga whales through the development of a behavioral catalog that lists the full repertoire of sexual and socio-sexual behaviors. A behavioral catalog was developed initially from 800 hours of observations, collected across a 7-year period from 11 belugas ranging in age (birth to 30+ years), sex, and social groupings. Using this behavioral catalog, observations of eight additional belugas housed between two other facilities were coded for socio-sexual and sexual behaviors. Socio-sexual and …

Abiotic Habitat Preferences Of Age-0 Paddlefish In Lake Sakakawea, North Dakota During Late Summer, Derek Dufault

Journal of Earth and Life Science

Since the early 1990’s, surface visual counts along transects have been conducted annually, allowing for estimates of relative abundance and year-class strength of age-0 paddlefish Polyodon spathula on the upper portions of Lake Sakakawea, North Dakota. This study focuses on the annual visual surface count data collected over an eleven year period from 2004-2014. More specifically it focuses on abiotic habitat factors (Secchi depth, water depth, and water temperature) and how they relate to age-0 paddlefish locations in late summer. Understanding habitat use during early life stages is imperative, yet to date, there is very limited knowledge available regarding the …

Influence Of Depth And Retrieval Speed On Yellow Perch Barotrauma Recovery Time In Winter, Bradley J. Klingsheim

Journal of Earth and Life Science

Physoclistous fish are often affected by barotrauma because the swim bladder is not connected to the esophagus. Barotrauma is increasingly being used in assessing mortality in fish brought from deep water. Barotrauma is the physical damage that occurs because of the over inflation of the gas bladder resulting from a rapid decrease in ambient pressure. The two important factors influencing barotrauma effects are water depth and decompression. In this study, two experiments were conducted on yellow perch (Perca flavescens) at three depth locations on Grace Lake, Bemidji, Minnesota. Fish were measured for barotrauma recovery time (s) following capture by hook …

Carnivore Diet Identification Through Scat And Genetic Analysis In Namibia, Africa, Alicia J. Walsh

Honors Theses and Capstones

Worldwide the cheetah population is declining making them Africa’s most endangered large cat. Namibia, Africa currently has the largest population of cheetahs in the world. During the summer of 2014, I did scat analysis of carnivores on the property of the Cheetah Conservation Fund (CCF) in Namibia in order to better understand the predators that compete with the cheetah and how the ecosystem works as a whole. I worked at CCF for nine weeks to analyze the diets of carnivores in the area through genetic and scat analysis. Analyzing carnivore feces would ultimately identify the diet of various carnivores in …

Assessing The Contributions Of Two Stocked Strains On A Minnesota-Wisconsin Border Water, Douglas Zentner

Journal of Earth and Life Science

Stocking has long been used as a management tool in the United States even though many of its effects remain cryptic. Past comparisons between stocked strains were generally based on the survival and growth rates of physically marked individuals. More recently the use of genetic markers to assess contributions of stocked individuals has been employed by fisheries managers. Using 13 microsatellite markers we assessed the contributions of two muskellunge (Esox masquinongy) strains (Wisconsin and Leech) stocked into the St. Louis River Estuary. Ancestry in the adult samples remained relatively constant with differences only observed between the 2008 and …

Using Wild I.D. As A Reliable Source For Mark And Recapture Studies On Northern Pike (Esox Lucius), Martin Evans

Journal of Earth and Life Science

Mark and recapture studies are a very popular method fisheries biologists use to assess certain fish populations in lakes. This process can be very labor intensive and expensive. Wild I.D. is free software developed by Dartmouth College that uses SIFT program to find unique features in photographs. Initially developed for identification of African land mammals the program gives each photo a score and percent match to other photos. Northern pike were used in this study to determine if the program can recognize simulated recapture events. Photos of sample fish were taken at two separate locations, the photos were then copied …

Juvenile Chinook Salmon Diet In Comparison To Invertebrate Drift In The Merced River, California, Tyler Orgon

Journal of Earth and Life Science

Diets and habitat types of salmonids have been widely studied in disturbed streams and rivers. The Merced River in California has a long history of flooding, damming, levee failures, and channel reconstruction events that has disrupted the aquatic ecosystem. This 30 day study was conducted in the Robinson Reach of the Merced River using juvenile Chinook salmon (Oncorhynchus tshawtscha). The objectives were to try and understand if juvenile Chinook salmon were selecting certain invertebrate species, determine if there were any differences in diets between habitat types, and determine whether diets and drift samples were different. Diets consisted of …

Effects Of Cooler Northern Waters On Growth Rates In Bluegill And Pumpkinseed Sunfish, Alec Spurbeck

Journal of Earth and Life Science

The Centrarchidae family range spans all across the United States making them a favorite target for many recreational anglers. In northern Minnesota, most water bodies do not reach optimal growth temperatures for the sunfishes, putting them right on the edge of their suitable range and potentially slowing overall growth. Therefore, the main objectives of this study were to 1) determine if sunfish growth rates respond to the severity of winter and 2) investigate growth differences between two sunfish species. Growth rates of bluegill (Lepomis macrochirus) and pumpkinseed (Lepomis gibbosus) were estimated from Big Sugar Bush Lake, …

Spatial And Temporal Factors Influencing Northern Pike Fry Density Estimates, Nate Stott

Journal of Earth and Life Science

The Tamarac River in northern Minnesota is an important spawning site for northern pike (Esox lucius) in the Red Lake system. However, little is known about fry production in the river. This study investigates migration densities and spatial and temporal factors influencing northern pike fry density estimates. Northern pike fry were collected with drift nets between 28 May and 4 June 2014. Thirty northern pike fry were randomly selected to have total length measured from each sample. Northern pike fry were observed in the first sample day. Northern pike fry density maxima occurred on 29 May at midnight …

A Genetic Based Approach To Management And Stocking Of Muskellunge, Douglas Zentner

Journal of Earth and Life Science

Fish stocking has long been used as a management tool among fisheries biologists and continues to be met with popular public opinion. Despite perceived simplicity, the full effects of stocking remain cryptic for many fish populations. Stocking has frequently been used to maintain or establish successful muskellunge (Esox masquinongy) populations. Coupled with other management practices the Minnesota Department of Natural Resources (MNDNR) has established Minnesota as a premier muskellunge fishery. From 1958 to 2012 the MNDNR stocked three strains of muskellunge in Big Mantrap Lake, MN. The first from Shoepack Lake, MN (hereafter Shoepack-strain) was used until the …

Behavioral Response Of Pacific Lamprey (Entosphenus Tridentatus) To Predator Odors, Laurie L. Porter

All Master's Theses

Pacific lamprey (Entosphenus tridentatus), a species facing serious threats to their existence, experience a number of challenges in reaching their desired spawning grounds during the adult migratory phase, and predators are suspected to be one of these challenges. Understanding if Pacific lamprey respond to predator odorants may provide a management tool for use in conjunction with attractants in guiding lamprey to suitable spawning habitat and deterring them from poor habitat. Previous research has failed to explore Pacific lamprey response to predator odorants, although much research exists on attractant odorants. In our study, we tested Pacific lamprey response to …

Effects Of River Current Inflow On Dissolved Oxygen And Temperature Of Refuge Zones For Cisco In Lake Bemidji, Bryant C. Swenson

Journal of Earth and Life Science

The objectives of this study were to (1) determine if the inflow current from the Mississippi River affects temperature and dissolved oxygen in Lake Bemidji, (2) determine how the amount of suitable cisco Coregonus artedi habitat changes over the summer in Lake Bemidji, and (3) predict how much cisco habitat would be available under a climate warming scenario. Summer fish kill of cisco occurs in most years on Lake Bemidji resulting from low dissolved oxygen in the hypolimnion and high water temperatures in the epilimnion. Dissolved oxygen and temperature profiles provided evidence to suggest the north basin is more suitable …

The Effect Of Barometric Pressure On Feeding Activity Of Yellow Perch, Daniel Vanderweyst

Journal of Earth and Life Science

This is the first study to our knowledge that has attempted to relate feeding activity of fish to barometric pressure. This study was conducted with the expectation that a difference in food ingestion would occur in a game fish species at different barometric pressures. The overall objective of this study is to determine if barometric pressure influences the feeding activity of a popular game fish. Yellow perch (Perca flavescens) were collected by seine and trap net from Lake Bemidji 12 September through 24 October 2013. The range in size and weight of yellow perch used for this study …

Use Of Cast Nets And Seine Hauls To Estimate Abundance Of Age-0 Yellow Perch, Michael Vaske

Journal of Earth and Life Science

Knowledge about fish population dynamics is essential for developing management plans and evaluating management success. In many lakes, yellow perch (Perca flavescens) are the main forage for many upper level predators and recreational fish species. To better manage recreational fisheries, an accurate estimate of prey abundance is necessary. The objectives of the study were to sample the littoral and limnetic zone to better estimate population of age-0 yellow perch, and obtain an estimate of the proportion of yellow perch being missed by traditional littoral seining methods. Littoral seines were conducted at three randomly selected locations in the south …

Changing Body Condition In Walleye With Reference To Ideal Sample Size Requirements, Kevin Longtin

Journal of Earth and Life Science

The objective of this study was to determine if walleye Sander vitreus percent dry weight, a measure of condition, differed among summer months of 2014 on Maple Lake, Minnesota. A second objective was to determine if there was a relationship between condition and body temperature, total length, or wet weight. Between three and six walleyes were collected per month by hook and line methods. All fish had internal body temperatures taken at the time of capture and were oven dried in the laboratory at 80 °C in order to measure percent dry weight. There was no relationship between condition and …

The Effects That Liquid And Solid Cattle Manure Have On The Water Quality Of Drainage Ditches In Putnam County, Ohio, Janelle Horstman

Honors Projects

Lake Erie has experienced harmful algal blooms with increased frequency since the mid-1990s due to excess nutrients from Rivers, such as the Maumee River, and largely agricultural watersheds. Nonpoint source pollution from agriculture contributes to eutrophication, algal blooms, and the degradation of water quality. This creates stress on aquatic fauna, reduced aesthetic quality, odor, and limits of the water for usage of drinking, recreation, and industry. This research paper asks what the contributions of having access to manure application records, soil records, and information about antibiotics have on what is known about manure management and antibiotic resistance, which has been …

Defining The Physiochemical, Biological And Habitat Requirements For The Upper Extant Of Oncorhynchus Mykiss Distribution, Emily Powers

Journal of Earth and Life Science

The distribution of rainbow trout is strongly influenced by their habitat. As climatic conditions change, the distribution of rainbow trout populations will change along with them. The objective of this study was to identify the particular habitat parameters that rainbow trout select for in this lotic system and to specifically assess how this relates to the upper extant of their distribution. By assessing the distribution of the upper extant specifically, future research may be conducted to better assess climatic changes on a small and/or large scale basis. A 9,000 m section of the Clearwater River was used as the study …

The Effects Of Angling Pressure On Northern Pike Size Structure, Ryan Carrow

Journal of Earth and Life Science

A number of conditions within a lake system can affect northern pike (Esox lucius) size structure. Of these conditions, the amount of angling pressure may have the biggest effect on the size structure of northern pike in Minnesota lake systems. In this study, five private and five public lakes were chosen near Bemidji, Minnesota. Each lake was fished for twenty angling hours using live northern hog suckers (Hypentelium nigricans) or golden shiners (Notemigonus crysoleucas). Total length (mm) and weight (kg) were measured for each northern pike caught. The number of fish houses that were …

Winter Survival Strategies Of Benthic Invertebrates In Temporary Habitats In A Northern Minnesota Lake, Cal Oldenburg

Journal of Earth and Life Science

During the winter months in the northern hemisphere, above 40 degrees latitude, a freezing event occurs that can last several months. Some lakes may have large portions of the shallow littoral zone frozen completely solid down into the sediments. Invertebrates affected by these events have developed survival strategies to cope with the harsh environment. The two main strategies for invertebrates in these areas are to either migrate to a different area and recolonize the original area when it becomes available again, or to burrow into the sediments and withstand the freezing. A 1- month study took place on Lake Bemidji …

Pcb Effects On Development Of Behavior In Rats, Robert Witte

Honors Projects

Abstract

PCBs were originally used as industrial chemicals in many devices and applications, including electrical capacitors and transformers, lubricants, and sealants in buildings. PCBs have been investigated and their use has been slowly phased out starting in the U.S. in the 1970s. A worldwide ban was put on the use and manufacturing of PCB in 2001, however they remain in the food chain because of their lipophilic properties and their long half-life. Major sources of PCB include wild-life, fish, and other seafood. Individuals exposed to PCB during development can experience negative effects on neurobiological, cognitive, and behavior functioning in both …