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Articles 181 - 210 of 248
Full-Text Articles in Exercise Science
Motor Function Responses To Induced Pain And Cryotherapy, Blaine Cletus Long
Motor Function Responses To Induced Pain And Cryotherapy, Blaine Cletus Long
Theses and Dissertations
Objective: Establish and validate an experimental pain model that will create pain for at least 20-minutes and then use the model to determine if: 1) cryotherapy decreases experimentally induced pain, 2) experimentally induced pain contributes to arthrogenic muscle inhibition, and 3) cold application influences pain or arthrogenic muscle inhibition. To answer these questions we conducted two experiments, the results of which are presented in two manuscripts. Methods: Seventy (n = 30 for experiment I and n = 40 for experiment II), physically active healthy male subjects participated. Interventions: Independent variables used for experiment I were condition (5% hypertonic saline infusion/cryotherapy, …
Children's Perceptions Of The Fitnessgram Fitness Test, Barbara Boone Sampson
Children's Perceptions Of The Fitnessgram Fitness Test, Barbara Boone Sampson
Theses and Dissertations
FITNESSGRAM is a battery of fitness tests that assess if a child's fitness level is, according to a health standard, enough to be considered healthy. These tests include the five components of health-related fitness: aerobic endurance, muscular strength and muscular endurance, flexibility, and body composition. Students are not compared to each other, but to health fitness standards specific to their age and gender that indicate good health. The purpose of this qualitative study was to identify children's perceptions of FITNESSGRAM and determine if self-administration of this fitness test provides a positive experience for the students. Specifically, this study evaluated (a) …
Physiological Differences Between Land And Water Treadmill Running, Rachel Kemp Rife
Physiological Differences Between Land And Water Treadmill Running, Rachel Kemp Rife
Theses and Dissertations
Objective: To determine if water treadmill running with (WTR-S) or without water shoes (WTR-NS) could produce similar cardiorespiratory responses as land treadmill running (LTR). Design and Setting: A repeated measures design was used to assess the differences between LTR and WTR-S and WTR-NS. All testing was done in either a research laboratory or an athletic training hydro-therapy room. Subjects: Eighteen trained runners (9 men and 9 women) volunteered for this study. All 18 subjects participated in three running conditions. Measurements: Treadmill speed, HR, and SF were assessed at four exercise intensities representing 50%, 60%, 70%, and 80% of land VO2max …
A Biomechanical Analysis Of Male And Female Intermediate Hurdlers And Steeplechasers, Laurence R. Bollschweiler
A Biomechanical Analysis Of Male And Female Intermediate Hurdlers And Steeplechasers, Laurence R. Bollschweiler
Theses and Dissertations
In the sport of track and field, proper hurdling technique is a complicated combination of various running and jumping kinematics. With most research having been done on sprint hurdling, there is a growing need for research on hurdling events of different lengths. The intermediate hurdles (IH) and the steeplechase (SC) are two events where there are a number of differences in hurdling technique. This study compared the differences in hurdling technique between events (IH and SC) as well as the differences in technique between genders. Subjects for this study consisted of 20 elite intermediate hurdlers (10 male, 10 female) and …
Exercise Induced Hypervolemia: Role Of Exercise Mode, William Bradley Nelson
Exercise Induced Hypervolemia: Role Of Exercise Mode, William Bradley Nelson
Theses and Dissertations
The supine posture has been shown to limit exercise-induced plasma volume expansion. Differences in hydrostatic pressure gradients between the standing and seated position indicate that treadmill exercise might promote a greater plasma volume expansion than cycle ergometer exercise. To test this hypothesis ten subjects performed intermittent high intensity exercise (4 min at 85% VO2max, 5 min at 40% VO2max repeated 8 times) on separate days on the treadmill and cycle ergometer. Changes in plasma volume expansion were calculated from changes in hematocrit and hemoglobin. Stroke volume (SV), trans-thoracic impedance (Z0), HR, and arterial blood pressure (non-invasive arm cuff, SBP & …
Comparison Of A Low Carbohydrate And Low Fat Diet For Weight Maintenance In Overweight Or Obese Adults Enrolled In A Clinical Weight Management Program, James D. Lecheminant, Cheryl A. Gibson, Debra K. Sullivan, Sandra Hall, Rik Washburn, Mary C. Vernon, Chelsea Curry, Elizabeth Stewart, Eric C. Westman, Joseph E. Donnelly
Comparison Of A Low Carbohydrate And Low Fat Diet For Weight Maintenance In Overweight Or Obese Adults Enrolled In A Clinical Weight Management Program, James D. Lecheminant, Cheryl A. Gibson, Debra K. Sullivan, Sandra Hall, Rik Washburn, Mary C. Vernon, Chelsea Curry, Elizabeth Stewart, Eric C. Westman, Joseph E. Donnelly
Faculty Publications
Background: Recent evidence suggests that a low carbohydrate (LC) diet may be equally or more effective for short-term weight loss than a traditional low fat (LF) diet; however, less is known about how they compare for weight maintenance. The purpose of this study was to compare body weight (BW) for participants in a clinical weight management program, consuming a LC or LF weight maintenance diet for 6 months following weight loss. Methods: Fifty-five (29 low carbohydrate diet; 26 low fat diet) overweight/obese middle-aged adults completed a 9 month weight management program that included instruction for behavior, physical activity (PA), and …
Effects Of Placement, Attachment, And Weight Classification On Pedometer Accuracy, Susan V. Graser, William J. Vincent, Robert P. Pangrazi
Effects Of Placement, Attachment, And Weight Classification On Pedometer Accuracy, Susan V. Graser, William J. Vincent, Robert P. Pangrazi
Faculty Publications
The purpose was to determine if waist placement of the pedometer affected accuracy in normal, overweight, and obese children, when attaching the pedometer to the waistband or a belt. Methods: Seventy-seven children (ages 10-12 years) wore 5 pedometers on the waistband of their pants and a belt at the following placements: navel (NV), anterior midline of the right thigh (AMT), right side (RS), posterior midline of the right thigh (PMT), and middle of the back (MB). Participants walked 100 steps on a treadmill at 80 m · min–1. Results: The RS, PMT, and MB sites on the waistband and the …
Plasma Volume And Albumin Mrna Expression In Exercise Trained Rats, Nathan Alex Bexfield
Plasma Volume And Albumin Mrna Expression In Exercise Trained Rats, Nathan Alex Bexfield
Theses and Dissertations
Introduction- Exercise-induced plasma volume (PV) expansion is typically associated with an increase in plasma albumin content. Increased hepatic albumin synthesis, a transcriptionally regulated process, is thought to contribute to the increase in albumin content. Objective- We tested the hypothesis that exercise training induces an increase in albumin gene expression in relationship to the increase in PV. Methods and Results- 40 adult male Sprague-Dawley rats weighing between 245-350 grams were randomly assigned to one of four groups: cage control (CC); sham exercise 10 min/day at 48% VO2max (NE); continuous exercise training, 60 min /day at 72% VO2max (LI); and high intensity, …
Junior High Students' Perceptions Of The Fitnessgram Fitness Test, Emily Mcomber Welch
Junior High Students' Perceptions Of The Fitnessgram Fitness Test, Emily Mcomber Welch
Theses and Dissertations
The Fitnessgram is a battery of fitness tests designed for children and adolescents. These tests include aerobic capacity, body composition, muscle strength and endurance, and flexibility. Students are not compared to each other, rather to health fitness standards, specific to age and gender, which indicate good health. The purpose of this study was to identify student perceptions of the Fitnessgram fitness test. This study used surveys (N=82), and follow-up focus-group interviews (N=16) to identify student perceptions of the Fitnessgram fitness test. Results using the constant comparative method revealed three major categories: (1) students' perceptions of the purpose of fitness testing, …
The Influence Of Performance Level And Setting On Collegiate Athletes' Motivational Profiles, Shareen Brooke Smith
The Influence Of Performance Level And Setting On Collegiate Athletes' Motivational Profiles, Shareen Brooke Smith
Theses and Dissertations
The purpose of this study was to determine if motivational profiles of individual collegiate team sport athletes differ across ability levels (High, Middle, and Low) and settings (Team Practice, Competition, and Personal Practice). The athletes task and ego disposition, autonomy, and contextual motivation, were assessed using the Perception of Success Questionnaire (POSQ), Sport Climate Questionnaire (SCQ), and Sport Motivational Scale (SMS), pre and postseason. Their anxiety levels and situational motivation were measured using the Sport Anxiety Scale (SAS) and the Situational Motivational Scale (SIMS) during the season. The General Causality Orientations Scale (GCOS) was used pre and postseason to assess …
Development Of A Notational Analysis System To Evaluate Setting Performance In Volleyball, Nina Puikkonen Mortensen
Development Of A Notational Analysis System To Evaluate Setting Performance In Volleyball, Nina Puikkonen Mortensen
Theses and Dissertations
The purposes of this study were to develop a notational analysis system to evaluate volleyball setting performance independent of the actions of the hitter and to use the data to develop a Markovian transitional matrix that would make known the probabilities of specific outcomes from each setting scenario. Setting performance was analyzed based on the sets distance from the net, height of the set, and position of the set in relation to the hitter as viewed from 13 filmed competitions of a Division I intercollegiate women's volleyball team. Data from the notation of 1353 sets were used to develop a …
The Effects Of The Front Squat And Back Squat On Vertical Jump And Lower Body Power Index Of Division 1 Male Volleyball Players, Manu Hoani Peeni
The Effects Of The Front Squat And Back Squat On Vertical Jump And Lower Body Power Index Of Division 1 Male Volleyball Players, Manu Hoani Peeni
Theses and Dissertations
The purpose of this study was to compare effects of the front squat and back squat on vertical jump and lower body power index of division 1 male volleyball players. Eighteen NCAA Division 1 male volleyball players volunteered for this study and were assigned either to a back squat or a front squat training program group. Subjects followed the training program for 8 weeks. Counter-movement vertical jump (CMVJ) height and lower body power index were measured at 0, 4 and 8 weeks of the study. A 2x3 factorial ANOVA revealed no significant difference in CMVJ height and power index between …
Dietary Fiber Intake And Body Fat Gain: A Prospective Cohort Study Of Middle-Aged Women, Kathryn Suzanne Thomas
Dietary Fiber Intake And Body Fat Gain: A Prospective Cohort Study Of Middle-Aged Women, Kathryn Suzanne Thomas
Theses and Dissertations
This study was conducted to determine the extent to which changes in dietary fiber consumption affect weight and body fat percentage (BF%) over time. An auxiliary objective was to examine the influence of age, total caloric intake, and physical activity (PA) on the relationship between changes in fiber intake and changes in body composition over time. Design/ Subjects: Prospective cohort design with baseline and follow-up assessments 20 months apart and 252 middle-aged women (40.1±3.0 y). Diet, particularly caloric and fiber intake, was measured using 7-day weighed food records. Body fat was assessed via the Bod Pod, and PA was measured …
C-Reactive Protein Levels According To Physical Activity And Body Weight For Participants In The Coronary Health Improvement Project, Michael T. Massey
C-Reactive Protein Levels According To Physical Activity And Body Weight For Participants In The Coronary Health Improvement Project, Michael T. Massey
Theses and Dissertations
Objectives. Evaluate C-reactive protein (CRP) levels according to weight and physical activity. The study explored how changes in CRP were associated with baseline CRP, weight, and physical activity and changes in these variables.
Methods. A randomized controlled study design assigned 348 individuals to the intervention or control group with measurements taken at baseline, 6 weeks, and 6 months of body weight, physical activity, and serum CRP levels. Participants attended an intensive 40-hour educational course delivered over a four-week period.
Results. At baseline, CRP was negatively associated with total steps/week, and positively associated with weight, BMI, percent fat, and saturated fat …
Ground Reaction Force Differences Between Running Shoes, Racing Flats, And Distance Spikes In Runners, Suzanna Jean Logan
Ground Reaction Force Differences Between Running Shoes, Racing Flats, And Distance Spikes In Runners, Suzanna Jean Logan
Theses and Dissertations
To measure the differences in ground reaction forces between running shoes, racing flats, and distance spikes, twenty intercollegiate distance runners ran across a force plate at 6.7m/s (for males) and 5.74m/s (for females) in each of the three types of shoes. In order to control for differences in foot strike, only subjects who had a heel strike were included in the data analysis (N=16). Repeated-measures ANOVA and Tukey's post-hoc test (p<0.05) revealed loading rate and impact peak to be significantly increased in the flats and spikes compared to running shoes. Stiffness in spikes was significantly higher than in running shoes. Stance time in spikes and flats was decreased. These results can be used to better inform competitive runners, coaches, and trainers of the risks and performance benefits when determining the frequency and duration of the use of competitive footwear in training.
Emotional Health And Weight Gain: A Prospective Study Of Middle-Aged Women, Ann Bahr
Emotional Health And Weight Gain: A Prospective Study Of Middle-Aged Women, Ann Bahr
Theses and Dissertations
The objective of this study was to investigate the extent to which risk of gaining weight or body fat is affected by emotional health in women. A secondary objective was to determine the influence of age, baseline weight and body fat, physical activity (PA), energy intake, and changes in PA and energy intake, on the relationship between emotional health and gains in weight and body fat. The study was a prospective cohort investigation over 20 months of 256 healthy, non-obese females (age: 35-45 y, BMI < 30 kg/m2). All subjects were assessed for several variables using objective measurements at baseline and again at 20 months. Emotional health was assessed using the General Well-Being Schedule. Body fat percentage was indexed using the Bod Pod. PA was measured objectively using MTI (CSA) accelerometers, and energy intake was measured using weighed, 7-day food records. The results of the study demonstrated that risk of gains in weight and body fat were no greater in depressed women compared to their counterparts. However, 171 (66.8%) subjects demonstrated less than positive (LTP) emotional health at baseline, and 37.4% of these subjects gained weight during the 20-month study. Conversely, 85 (33.2%) subjects had positive emotional health at baseline, but only 23.5% gained weight over the investigational period. With no variables controlled, women with LTP emotional health had 59% greater risk of weight gain over the study period than women with positive emotional health (RR 1.59, 95% CI = 1.04-2.44). Women with LTP emotional health were at no greater risk of gains in body fat percentage than women with positive emotional health (RR 0.96, 95% CI = 0.70-1.33). After adjusting for each potential confounder individually, risk of gaining weight or body fat did not change. However, after adjusting for all of the potential confounders simultaneously, risk of weight gain was weakened (RR 1.43, 95% CI = 0.93-2.21). These results seem to demonstrate that middle-aged women with LTP emotional health may be at increased risk of gaining weight compared to women with positive emotional health.
Whole-Body Vibration Compared To Traditional Physical Therapy In Individuals With Total Knee Arthroplasty, Aaron W. Johnson
Whole-Body Vibration Compared To Traditional Physical Therapy In Individuals With Total Knee Arthroplasty, Aaron W. Johnson
Theses and Dissertations
The purpose of the present study was to compare total knee arthroplasty (TKA) rehabilitation with and without whole-body vibration (WBV) to 1) understand if WBV is a useful treatment during TKA rehabilitation to increase quadriceps strength and function, and 2) to investigate the effect of WBV on quadriceps voluntary muscle activation. Subject and Methods. Individuals post TKA (WBV n=8, control n=8) received physical therapy with and without WBV for four weeks. Quadriceps strength and muscle activation, function, perceived pain, and knee range of motion were measured. Results. No adverse side effects were reported in either group. There was a significant …
The Relationship Between Maternal Employment And Children's Physical Activity, Michael Scott Parker
The Relationship Between Maternal Employment And Children's Physical Activity, Michael Scott Parker
Theses and Dissertations
The purpose of this study was to determine children's and parents' activity levels, examine the relationship between mothers' and children's mean step counts, and fathers' and children's mean step counts, and determine if there are differences in physical activity levels among children with mothers who work full time, work part time, or are not employed. Fifty-eight families participated in this 12-day study. Each family member wore a Walk4Life LS 2525 or a Walk4Life LS 2505 pedometer to measure daily step counts. Additionally, all participants completed the pedometer step count logs each night, and parents completed a short demographic form. In …
Acute Stretch Perception Alteration Contributes To The Success Of The Pnf "Contract-Relax" Stretch, J. Brent Feland, Sterling C. Hilton, J. Ty Hopkins, Iain Hunter, Ulrike H. Mitchell, J. William Myrer
Acute Stretch Perception Alteration Contributes To The Success Of The Pnf "Contract-Relax" Stretch, J. Brent Feland, Sterling C. Hilton, J. Ty Hopkins, Iain Hunter, Ulrike H. Mitchell, J. William Myrer
Faculty Publications
Context: Some researchers have suggested that an alteration of stretch perception could be responsible for the success of the contract-relax (CR) stretch, a stretch technique derived from proprioceptive neuromuscular facilitation (PNF). Objective: This study was conducted to determine if the alteration of the stretch perception is a possible explanation for the range of motion (ROM) gains of the CR stretch. Participants: Eighteen subjects performed two stretches in randomized order: the slow stretch and the CR stretch. Main Outcome Measure: The stretch intensity was controlled. The stretch force was measured and compared between the slow stretch and CR stretch. Results: There …
Technique And Performance Level Comparisons Of Male And Female Hammer Throwers, Suzanne M. Konz
Technique And Performance Level Comparisons Of Male And Female Hammer Throwers, Suzanne M. Konz
Theses and Dissertations
The aim of this study was two-fold: 1) what the hammer throwing technique differences between sexes are and 2) what technique parameters help determine throw distance. The performances of the top 16 male and female throwers at the 2003 World Athletic Final and the top 13 male and female throwers from the 2003 USA Track and Field Nationals were examined. Video was captured using three Canon 60 Hz cameras. The best throws of each athlete were digitized and analyzed using the Peak Motus 8.2 motion analysis system. T-tests revealed that athlete mass, athlete height, velocity at release, timing components, and …
Effects Of Telephone Weight Loss Coaching On Body Composition In Adults: A Randomized Controlled Study, Amy Jensen Cook
Effects Of Telephone Weight Loss Coaching On Body Composition In Adults: A Randomized Controlled Study, Amy Jensen Cook
Theses and Dissertations
Objective: To determine the extent to which coaching over the telephone is an effective method in promoting the loss of body weight and body fat percentage. Research Methods and Procedures: Over a period of four months, in a randomized, double blind, placebo-controlled study, 120 overweight and obese (BMI 25-35 kg/m²) adults either received telephone coaching or no coaching. In addition, each participant was randomly assigned to take a supplement or placebo daily. Body weight and body fat percentage were measured at baseline, two months, and four months. Body weight was measured on an electronic scale and body fat percentage was …
Warming Up With An Ice Vest: Core Body Temperature Before And After Cross-Country Racing, J. Ty Hopkins, Iain Hunter, Douglas J. Casa
Warming Up With An Ice Vest: Core Body Temperature Before And After Cross-Country Racing, J. Ty Hopkins, Iain Hunter, Douglas J. Casa
Faculty Publications
Context: Athletes running in a hot, humid environment may have an increased risk of heat illness. In the 2004 Olympic Games, American and Australian athletes were provided with ice vests designed to cool their bodies before performance. The vest appeared to be effective in keeping body temperatures down and improving the performance of the marathoners. However, body temperatures have not been reported when the vest was used before an actual competition. Objective: To determine if wearing the Nike Ice-Vest decreased core temperature (Tc) before and during athletic performance in warm (26°C to 27°C), humid (relative humidity = 50% to 75%) …
The Effects Of Wearing A Cooling Vest During The Warm-Up On Long Distance Interval Training, Andrew Robert Tegeder
The Effects Of Wearing A Cooling Vest During The Warm-Up On Long Distance Interval Training, Andrew Robert Tegeder
Theses and Dissertations
Purpose: Reducing body temperature before exercise is called precooling. Past research suggests that reducing body core temperature (Tc) slightly can result in improved running performance. This study evaluated the effects that warming up, while wearing a cooling vest prior to an interval workout, had on Tc, and interval time in long-distance runners. Methods: Nineteen healthy male collegiate cross-country runners were recruited for this study. Each subject warmed up and exercised under two different conditions: (a) an experimental condition in which subjects wore a Nike PreCool® ice vest during warm-up and (b) a control condition. Subjects performed a warm-up followed by …
Contributions Of Muscle Fatigue To A Neuromuscular Neck Injury In Female Standard Ballroom Dancers, Teri Riding
Contributions Of Muscle Fatigue To A Neuromuscular Neck Injury In Female Standard Ballroom Dancers, Teri Riding
Theses and Dissertations
Objective: To investigate the potential etiology of a loss of neck control injury in female standard ballroom dancers. The median frequency (MF) as measured by electromyography (EMG) of the left upper trapezius (UT), left splenius capitius (SPL), and right sternocleidomastoid (SCM) of injured dancers was compared to non-injured dancers. This comparison was performed to identify whether dancers with a history of loss of neck control have a greater amount of fatigue than those with no history of this particular injury. Design and Setting: A 2 x 6 factorial design was used for this investigation. The independent variables were group (injured …
Development Of A Notational Analysis System For Selected Soccer Skills Of A Women's College Team, Camille Thomas
Development Of A Notational Analysis System For Selected Soccer Skills Of A Women's College Team, Camille Thomas
Theses and Dissertations
The purposes of this study were to develop a notational system to evaluate passing, dribbling, first touch, and individual defensive skills as they relate to success during women's soccer games and to develop a statistical model to weight the importance of each skill on creating scoring opportunities. Sequences of skills in 10 Division I intercollegiate women's soccer games were coded using well defined performance scores and outcomes. The notational analysis system was highly reliable as demonstrated by high test-retest Spearman's correlations (>0.98) between the first and second notation of 3 games for all four skills. The importance scores calculated …
Presence Of Burnout In Undergraduate Athletic Training Students, Tamra Sparks Riter
Presence Of Burnout In Undergraduate Athletic Training Students, Tamra Sparks Riter
Theses and Dissertations
Objective: To determine if undergraduate athletic training students enrolled in an athletic training education program (ATEP) and participating in a clinical assignment have burnout and if so, the possible causes.
Design and Setting: All undergraduate athletic training students enrolled in a clinical education course were surveyed twice in an eight week period. The surveys were given during the fourth week and the twelfth week of the winter 2006 semester. As part of the survey, each participant also answered eight demographic/status questions for correlation purposes.
Subjects: Fifty-one undergraduate athletic training students in a western United States university's accredited ATEP served as …
Effects Of Ankle Joint Cooling On Peroneal Short Latency Response, J. Ty Hopkins, Iain Hunter, Todd Mcloda
Effects Of Ankle Joint Cooling On Peroneal Short Latency Response, J. Ty Hopkins, Iain Hunter, Todd Mcloda
Faculty Publications
While cryotherapy has direct physiological effects on contractile tissues, the extent to which joint cooling affects the neuromuscular system is not well understood. The purpose of the study was to detect changes in ankle dynamic restraint (peroneal short latency response and muscle activity amplitude) during inversion perturbation following ankle joint cryotherapy. A 2x3 factorial design was used to compare reaction time and EMG amplitude data of treatment conditions (cryotherapy and control) across time (pre-treatment, post-treatment, and 30 min post-treatment). Thirteen healthy volunteers (age 23 ± 4 yrs, ht 1.76 ± 0.09 m, mass 78.8 ± 16.6 kg), with no history …
Steeplechase Barriers Affect Women Less Than Men, Tyler D. Bushnell, Iain Hunter
Steeplechase Barriers Affect Women Less Than Men, Tyler D. Bushnell, Iain Hunter
Faculty Publications
Women began contesting the 3000 m steeplechase during the 1990’s using barriers of different dimensions than men. Whenever a new event is introduced for women, consideration should be taken as to whether different technique or training methods should be utilized. This study investigated three aspects of hurdling technique: 1) Differences in the ratio of the landing step to the penultimate step between men and women around each non-water jump steeplechase barrier, 2) differences in step lengths between the four non-water jump barriers, and 3) changes in the step lengths around the barrier throughout the race. The step lengths around the …
Physical Activity And C-Reactive Protein Levels: The Confounding Role Of Body Fat Percentage, Kenric Lloyd Russell
Physical Activity And C-Reactive Protein Levels: The Confounding Role Of Body Fat Percentage, Kenric Lloyd Russell
Theses and Dissertations
The purpose of the present study was to examine the cross-sectional relationship between physical activity and C-reactive protein (CRP) in 211 middle-aged women (43.1 + 3.0 years). A secondary objective was to determine the extent to which body fat percentage operated as a confounder in the association between physical activity and CRP. Physical activity was objectively measured using MTI accelerometers, which the subjects wore for seven continuous days. Fasting blood samples were taken, from which CRP was measured using a solid phase ELISA. Body fat percentage was assessed using the Bod Pod. Results showed that physical activity was significantly and …
Fiber Type-Specific Desmin Content In Human Single Muscle Fibers, Heidi Ghent
Fiber Type-Specific Desmin Content In Human Single Muscle Fibers, Heidi Ghent
Theses and Dissertations
Contractile and cytoskeletal protein concentrations have been shown to differ on the basis of fiber type in whole muscle homogenates. The purpose of this study was to compare the content of the intermediate filament protein, desmin, between type I and type IIa single muscle fibers from a mixed muscle in human subjects. Biopsies were taken from the vastus lateralis of six recreationally active males. Approximately 150 single muscle fibers were dissected from each sample and analyzed using SDS-PAGE to determine myosin heavy chain (MHC) composition. Following identification, muscle fibers were pooled into two groups (MHC I and MHC IIa). Desmin …