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Articles 31 - 36 of 36
Full-Text Articles in Parasitology
Phylogenetic Analysis Of The Rhabdocoela (Platyhelminthes) With Emphasis On The Neodermata And Relatives, David Zamparo, Daniel R. Brooks, Deborah A. Mclennan, Eric P. Hoberg
Phylogenetic Analysis Of The Rhabdocoela (Platyhelminthes) With Emphasis On The Neodermata And Relatives, David Zamparo, Daniel R. Brooks, Deborah A. Mclennan, Eric P. Hoberg
Harold W. Manter Laboratory of Parasitology: Faculty Publications
Phylogenetic systematic analysis of 24 taxa representing the rhabdocoel platyhelminths, based on a suite of 89 morphological characters, produced two equally parsimonious trees, 181 steps long, with a consistency index (CI) of 0.69 and a rescaled consistency index (RCI) of 0.56, differing only with respect to that portion of the tree containing Umagillidae, Acholadidae, Graffillinae, Pseudograffillinae, Pterastericolidae and Hypoblepharinidae. Our results accommodate all previously proposed sister taxa to the Neodermata in a single clade in which ((Dalyelliidae + Temnocephalida) Typhloplanidae) is the sister group of ((Fecampiidae + Urastoma) (Udonella ((Aspidogastrea + Digenea) (Monogenea (Gyrocotylidea (Amphilinidea + Eucestoda)))))). Bootstrap …
Prevalence Of Eimeria (Apicomplexa: Eimeriidae) In Reintroduced Gunnison's Prairie Dogs (Cynomys Gunnisoni), M. M. Ryan, K. H. Decker, Donald W. Duszynski
Prevalence Of Eimeria (Apicomplexa: Eimeriidae) In Reintroduced Gunnison's Prairie Dogs (Cynomys Gunnisoni), M. M. Ryan, K. H. Decker, Donald W. Duszynski
Harold W. Manter Laboratory of Parasitology: Faculty Publications
Fecal samples from 54 Gunnison’s prairie dogs (Cynomys gunnisoni) from Albuquerque, NM were analyzed for the presence of coccidia and all were positive. They were then relocated to an abandoned prairie dog town on the Sevilleta Long Term Ecological Research (LTER) site. Six Eimeria species, E. callospermophili, E. cynomysis, E. pseudospermophili (new host record), E. spermophili, E. ludoviciani and E. vilasi (new host record) were found in Albuquerque animals, but only two species, E. callospermophili and E. vilasi were present in relocated hosts. A significant (P < 0.05) reduction was seen in the prevalence of E. vilasi (72% vs. 13%) and in the prevalence of infections …
Phylogey Of Steinernema Travassos, 1927 (Cephalobina: Steinnematidae) Inferred From Ribosomal Dna Sequences And Morphological Characters, S. Patricia Stock, James F. Campbell, Steven A. Nadler
Phylogey Of Steinernema Travassos, 1927 (Cephalobina: Steinnematidae) Inferred From Ribosomal Dna Sequences And Morphological Characters, S. Patricia Stock, James F. Campbell, Steven A. Nadler
Harold W. Manter Laboratory of Parasitology: Faculty Publications
Entomopathogenic nematodes in Steinernema, together with their symbiont bacteria Xenorhabdus, are obligate and lethal parasites of insects that can provide effective biological control of some important lepidopteran, dipteran, and coleopteran pests of commercial crops. Phylogenetic relationships among 21 Steinernema species were estimated using 28S ribosomal DNA (rDNA) sequences and morphological characters. Sequences of the rDNA internal transcribed spacers were obtained to provide additional molecular characters to resolve relationships among Steinernema carpocapsae, Steinernema scapterisci, Steinernema siamkayai, and Steinernema monticolum. Four equally parsimonious trees resulted from combined analysis of 28S sequences and 22 morphological characters. Clades inferred from …
Identification Of A Defensin From The Hemolymph Of The American Dog Tick, Dermacentor Variabilis, Robert Johns, Daniel E. Sonenshine, Wayne L. Hynes
Identification Of A Defensin From The Hemolymph Of The American Dog Tick, Dermacentor Variabilis, Robert Johns, Daniel E. Sonenshine, Wayne L. Hynes
Biological Sciences Faculty Publications
Hemolymph from partially fed virgin Dermacentor variabilis females was collected following Borrelia burgdorferi challenge and assayed for antimicrobial activity against Bacillus subtilis and B. burgdorferi. A small inducible cationic peptide was identified by SDS-PAGE in the hemolymph of these ticks as early as 1h post challenge. Following purification by a three-step procedure involving sequential SepPak elution, reversed phase high performance liquid chromatography (RP-HPLC) and gel electrophoresis, the yield of the active peptide was approximately 0.1% of the total protein in the hemolymph plasma. The molecular weight, 4.2kDa, was determined by MALDI-TOF mass spectrometry. N-terminal sequencing by the Edman degradation …
Infection And Transovarial Transmission Of Rickettsiae In Dermacentor Variabilis Acquired By Artificial Feeding, K. R. Macaluso, Daniel E. Sonenshine, Shane M. Ceraul, A. F. Azad
Infection And Transovarial Transmission Of Rickettsiae In Dermacentor Variabilis Acquired By Artificial Feeding, K. R. Macaluso, Daniel E. Sonenshine, Shane M. Ceraul, A. F. Azad
Biological Sciences Faculty Publications
In this study we examined the efficiency of an in vitro feeding technique using glass microcapillaries as a method of establishing rickettsiae-infected lines of ticks. To quantify the volume ingested by ticks during microcapillary feeding, the incorporation of radiolabeled amino acids in tick gut and hemolymph was calculated. Fifteen of 18 ticks consumed between 0.06 μl and 6.77μl. However, ingestion of fluid was not correlated to weight gain during capillary feeding. Uninfected and partially fed laboratory-reared female Dermacentor variabilis ticks were exposed to either Rickettsia montana- or Rickettsia rhipicephali-infected Vero cells via microcapillary tubes, returned to rabbit hosts, …
Properties Of A Factor In Eland Plasma That Inhibits Transformation Of Bloodstream-Form Trypanosoma Brucei Brucei, E. K. Nguu, E. O. Osir
Properties Of A Factor In Eland Plasma That Inhibits Transformation Of Bloodstream-Form Trypanosoma Brucei Brucei, E. K. Nguu, E. O. Osir
All Peer-Reviewed Publications
Fresh plasma from the African eland, Taiirotragus oryx, contains a factor that inhibits the transformation of bloodstream-form Trypanosma bnicei bntcei into procyclic (midgut) forms. Heating of the plasma at 42, 50 and 60 °C for 30 min resulted in a 20, 38 and 40% loss of inhibitor activity respectively, whereas only negligible loss occurred below 42 C. Similarly, one and four freeze-thawing cycles resulted in 32 and 60% loss of activity respectively. Inactivation of the inhibitor activity, which occurred rapidly during storage (for example, 80% loss after 7 days at -20 °C) could not be stopped by the addition of …