Immunopathology Commons^™

Rapid Identification Of Streptococcal Infections Using Fluorescent Antibody Techniques, James H. Meadows

Biology ETDs

The use of fluorescent microscopy coupled with immunological techniques offer new methods for the demonstration of antigen-antibody reactions. The adaptation of these techniques to grouping of beta hemolytic streptococci makes feasible their further adaptation toward identification of group A streptococci in clinical material. Commercially available labeled antibodies against group A beta hemolytic streptococci were obtained for use in this study. A method for the determination of labeled antisera specificity is proposed and a technique for absorbing out any heterologous reacting antibody is described. Using this commercial labeled antisera, subsequent to specificity studies plus absorption if necessary, adaptations of basic fluorescent …

A Study Of The Relation Of Temperature To Antibody Formation In Cold-Blooded Animals, Earl C. Mcdaniel

Biology ETDs

It is an established fact that when warm-blooded animals are inoculated with foreign protein, in a great many instances they become immune, due to the formation of antibodies specific for the protein injected. In warm-blooded animals the body temperature is constant. The question naturally arises as to what the situation would be with regard to antibody formation in a cold-blooded animal where body temperature is not constant; also what part, if any, the temperature of the body would have to do with the antibody formation.

In a careful review of the literature practically nothing could be found in having direct …