Immunology and Infectious Disease Commons^™

Use Of Pollen Concentration In Paleopharmacology: Coprolite Evidence Of Medicinal Plants, Karl Reinhard, Donny L. Hamilton, Richard H. Hevly

Karl Reinhard Publications

Nearly 2,400 plant species have been documented ethnographically as having medicinal value among Native Americans. Therefore, it is not surprising that evidence of medicinal plants appears in some of the over 1,000 coprolites analyzed from the southwestern U.S. Three medicinal species identified in pollen analysis of coprolites are discussed. Willow (Salix) is the most common analgesic in the Native American pharmacopoeia. Prehistoric use of this plant is documented in coprolites from Bighorn Cave in the Black Mountains of Arizona and also in a burial from the Mimbres Valley, New Mexico. Historically, Mormon tea (Ephedra) served as …

Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young

All Dugoni School of Dentistry Faculty Articles

Perforin lyses cells by binding to the target cell membrane, where it polymerizes into large nonspecific pores. It is shown here that the first 34 amino acids of the N-terminal region of either human or murine perforin are soluble in aqueous medium and spontaneously insert into membranes. The N-terminal peptides lyse liposomes and nucleated cells, and they form ion channels in planar bilayers, some of which are comparable to those previously described for perforin. The lytic activity of the N-terminal domains does not require calcium, is independent of the lipid headgroup composition, and can be inhibited by heparin. Tumor cells …

Development And Characterization Of Monoclonal Antisperm Antibodies: Potential For Contraception, Dilrowshan H. Haque

Theses and Dissertations in Biomedical Sciences

There is an expressed need to develop a greater variety of safe contraceptive methods which would find acceptance worldwide, particularly in developing countries. Immunization against spermatozoa might be such a method as judged by the accumulated evidence from studies on human females and in a number of female animal species.

Two extraction techniques were used for sperm membrane antigen isolation. The first technique involved NP-40 detergent for antigen extraction from human motile sperm and the second technique employed homogenized human testis for antigen extraction. Using these immunogens, sperm membrane-specific monoclonal antibodies (MAbs) were developed. When these antisperm MAbs were subjected …

Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

An alternative approach to structurefunction analysis of vesicular stomatitis virus (VSV) gene products and their interactions with one another during each phase of the viral life cycle is described. We showed previously by using the vaccinia viruslT7 RNA polymerase expression system that when cells expressing the nucleocapsid protein (N), the phosphoprotein (NS), and the large polymerase protein (L) of VSV were superinfected with defective interfering (DI) particles, rapid and efflicient replication and amplification of DI particle RNA occurred. Here, we demonstrate that all five VSV proteins can be expressed simultaneously when cells are cotransfected with plasmids containing the matrix protein …

Pharmacokinetics, Immune Response, And Biodistribution Of Iodine-131-Labeled Chimeric Mouse/Human Igg1, K 17-1a Monoclonal Antibody, Ruby Meredith, Albert Lobuglio, Walter Plott, Roger Orr, Ivan Brezovich, Charles Russell, Elizabeth Harvey, Michael Yester, Mansoor Saleh

Haematology and Oncology, East Africa

Pharmacokinetics,immunogenicity,and biodistributionof a 131I-Iabeledmouse/human chimenc monoclonal antibody (C17-1A) was studiedin six metastaticcoloncancerpatients. Pharmacokinetics obtalned from serum radioactivity or chi mera concentration were identical after 5 mCi of 131I(>17-1A withmeanalphahalf-livesof 17.6±2.3 and19.7±2.9 and meanbeta half-livesof 100.9±16.1 and 106.4±14.1 hr, respectively. HPLC analysis documented the monomeric chi rneiic17-lA withoutevidenceof immunecomplexesorfree 1311 None of the patients developed antibody after ‘31I-chi merle17-1A exposure.Radiolocalizationoccurredin known areas of disease >4 cm in all patients. The half-life of total body radioactivity was 58 ±7 hr by whole-body counts and 64 ±13 hr by urine measurements. Whole-body and bone marrow dose estimates ranged from 0.75-1 .03 and …

Pathogenicity Of Tsetse-Transmitted Trypanosoma Congolense For Waterbuck (Kobus Defassa) And Boran Cattle (Bos Indicus), R. O. Olubayo, S. Mihok, D. F. Wesonga, E. R.M. Mbwabi

All Peer-Reviewed Publications

Five waterbuck (Kobus defassa) and four Boran cattle (Bos indicus) were infected with Trypanosoma congolense IL2895 using Glossina morsitans morsitans. At the same time, two waterbuck and two cattle were inoculated intravenously with bloodstream forms. With both methods of challenge, cattle had short prepatent periods followed by a continuous high parasitaemia. All cattle became severely anaemic and had to be treated with trypanocidal drugs to prevent death. In contrast, tsetse and intravenous challenge of waterbuck resulted in a long prepatent period, followed by brief, intermittent levels of low parasitaemia, and eventual selfcure. Waterbuck did not become anaemic, even during short …

Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Five monoclonal antibodies specific for bovine respiratory syncytial virus were characterized by Western immunoblotting, radioimmunoprecipitation, and epitope mapping assays. The monoclonal antibodies were found to be specific for the fusion protein, and there were at least two antigen binding sites, one of which was neutralizing.

Dietary And Parasitological Analysis Of Coprolites Recovered From Mummy 5, Ventana Cave, Arizona, Karl Reinhard, Richard H. Hevly

Karl Reinhard Publications

Four coprolites were excavated with Burial 5 at Ventana Cave. a partially mummified five-year-old child. Two coprolites were granular and dark in color and two were fibrous and light in color. The coprolites are remains of the child's intestinal contents and were submitted for dietary and parasitological analysis. No parasites were found. The fibrous coprolites proved to be remains of highly masticated mesquite pods (Prosopis). The granular coprolites consist of seeds of saguaro cactus (Carnegiea gigantea). Pollen analysis reveals two dietary pollen types, both derived from cactus. No evidence of cultivated plants except for a trace amount of …

Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication and amplification of RNA genomes of defective interfering (DI) particles of vesicular stomatitis virus (VSV) depend on the expression of viral proteins and have untD now been attained only in ceUs coinfected with helper VSV. In the work described in this report, we used a recombinant vaccinia virus-T7 RNA polymerase expression system to synthesize individual VSV proteins in cells transfected with plasmid DNAs that contain cDNA copies of the VSV genes downstream of the T7 RNA polymerase promoter. In this way, we were able to examine the ability of VSV proteins, individually and in combination, to support DI particle …

Binder 147, Opecoelidae He-J [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Binder 147, Opecoelidae He-J [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 155, Opecoelidae Plagioporinae M-N [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Binder 155, Opecoelidae Plagioporinae M-N [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 163, Paramphistomatidae From Fishes K-Z [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Binder 163, Paramphistomatidae from Fishes K-Z [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 167, Paramphistomatidae From Mammals F-O [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Binder 167, Paramphistomatidae from Mammals F-O [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 171, Plagiorchiidae Plagiorogia Plagiorogia A-Z [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Binder 171, Plagiorchiidae Plagiorogia Plagiorogia A-Z [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 193, Strigeidae D-Z [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 193, Strigeidae D-Z [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 200, Small Families [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 200, Small families [Trematoda Taxon Notebooks], including: Diplangidae, Zoogonidae, Deropristiidae, Lepocreadiidae, Acanthocolpidae, Heronimidae, Liolopidae, and Harmotrematidae.

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 208, Life Histories E-G [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 208, Life histories E-G [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

Created between 1960 and 1990.

Binder 199, Small Families [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 199, Small families [Trematoda Taxon Notebooks], including: Haploporidae, Atractotrematidae, Batrachotrematidae, Brauninidae, Botulieaccidae, Nonorchiidae, Fellodistomatidae, Botulisaccidae, Callodistomidae, Callodistomatidae, and Allocreadiidae.

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 010, Allocreadiinae [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 020, Allocreadiinae [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 024, Bucephaloides [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 024, Bucephaloides [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 014, Aspidogastridae [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 014, Aspidogastridae [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 029, Bucephalidae M-T [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 029, Bucephalidae M-T [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 015, Azygiidae [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 015, Azygiidae [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 036, Cryptogonimidae H-O [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 036, Cryptogonimidae H-O [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 050, Dicrocoelidae Q-Z [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 050, Dicrocoelidae Q-Z [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 065, Echinostomatidae O-Pat [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 065, Echinostomatidae O-Pat [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

Created between 1960 and 1990.

Binder 066, Echinostomatidae Pau-Pz [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 066, Echinostomatidae [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

Created between 1960 and 1990.

Binder 085, Halpoporidae A-I [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 085, Haploporidae A-I [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 090, Hemiuridae Hemiurinae N-Z [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Abstract

Binder 090, Hemiuridae Hemiurinae N-Z [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.

Binder 091, Hemiuridae Aphanurinae A-Z [Trematoda Taxon Notebooks], Harold W. Manter Laboratory Of Parasitology

Trematoda Taxon Notebooks

Binder 091, Hemiuridae Aphanurinae A-Z [Trematoda Taxon Notebooks].

Harold W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln.

Created between 1960 and 1990.