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Articles 1 - 26 of 26
Full-Text Articles in Genetics
Filtered Genome Of Commercial Philippine Varieties Of Sugarcane (Saccharum Officinarum L.) For The Characterization And Identification Of Ssrs Associated With Resistance To Philippine Downy Mildew (Peronosclerospora Philippinensis), Antonio G. Lalusin, Jhun Lawrence S. Rasco
Filtered Genome Of Commercial Philippine Varieties Of Sugarcane (Saccharum Officinarum L.) For The Characterization And Identification Of Ssrs Associated With Resistance To Philippine Downy Mildew (Peronosclerospora Philippinensis), Antonio G. Lalusin, Jhun Lawrence S. Rasco
The Philippine Agricultural Scientist
Sugarcane is a major global source of sugar, with total production reaching 1.89 billion t. Although the Philippines produces about 1.85 million t of sugarcane annually, yields are limited by downy mildew and smut. Developing varieties resistant to these pathogens is the most economical and practical approach to mitigating losses from these biotic stresses. In this study, SSRs related to disease resistance were developed through Next-Generation Sequencing (NGS) of the filtered genomes of sugarcane varieties with known phenotypic responses to downy mildew. Two assemblies based on minimum contig lengths (450 and 900 bp) were performed using SoapDeNovo2. The 900-bp assembly …
Genetic And Geographic Profiling Of Fragrance-Associated Badh2 Mutations In Rice From China, Laos, And Vietnam, Lenie A. Quiatchon-Baeza, Venea Dara Daygon, Mariafe N. Calingacion, Melissa A. Fitzgerald
Genetic And Geographic Profiling Of Fragrance-Associated Badh2 Mutations In Rice From China, Laos, And Vietnam, Lenie A. Quiatchon-Baeza, Venea Dara Daygon, Mariafe N. Calingacion, Melissa A. Fitzgerald
The Philippine Agricultural Scientist
Fragrance in rice is a culturally and economically significant trait in Asia and around the world. This study aimed to develop a genetic and geographic profile of the fragrance-associated BADH2 mutations (badh2.1 and badh2.11) in 1,067 rice varieties from China, Laos, and Vietnam, which are part of the International Rice Genebank Collection. Seventy-three varieties were found to carry the common eight-base pair (bp) deletion in exon 7 of the genes (badh2.1, also known as badh2.7), primarily from indica varieties, with some belonging to japonica, javanica, and intermediate varieties. Conversely, 59 varieties possess the recently identified badh2 allele, badh2.11 (also known …
Impact Of Sowing Time And Plant Density On Selected Traits And Yield Of Cotton Genotypes In Pakistan, Muhammad Rizwan Jamil, Kalim Ullah, Muhammad Mohibullah, Saeed Muhammad, Hafiz Abdul Haq, Sundas Batool, Rashida Aslam, Wasif Rasool
Impact Of Sowing Time And Plant Density On Selected Traits And Yield Of Cotton Genotypes In Pakistan, Muhammad Rizwan Jamil, Kalim Ullah, Muhammad Mohibullah, Saeed Muhammad, Hafiz Abdul Haq, Sundas Batool, Rashida Aslam, Wasif Rasool
The Philippine Agricultural Scientist
Extremely diversified cropping environments prevail in most cotton-growing areas of Pakistan due to prevailing rainfed conditions, prolonging the crop’s cultivation period. To contribute to the development of desired breeding traits for effective breeding programs, this study examined the production and growth pattern of 8 cotton genotypes. Four experiments were conducted in 2018 and 2019 at 2 locations in Dera Ismail Khan, Pakistan, to evaluate the performance of the studied genotypes at 3 plant densities and 2 sowing dates. Traits such as days to first flower opening, flowering duration, monopodial branches per plant, plant height at harvest, height-to-node ratio, fruiting branch …
A Review On The Cytogenetic Integrity Of Micropropagated Plants [Review Paper], Eliaza Alumbro Peniton Jr
A Review On The Cytogenetic Integrity Of Micropropagated Plants [Review Paper], Eliaza Alumbro Peniton Jr
The Philippine Agricultural Scientist
Cytogenetic stability is essential for the survival of micropropagated plants. While micropropagation allows rapid propagation of plants with desirable phenotypes, it may also produce genetic variations that are, at times, cytogenetically observable. Factors affecting these variations are not fully understood, and micropropagation protocols are optimized to produce genetically stable regenerants. The genetic stability of micropropagated plants is traditionally assessed by comparing the phenomorphological traits of the regenerants to the mother plant. However, genetic variation does not often result in obvious morphological changes. Therefore, molecular and cytogenetic markers are typically used to evaluate the genetic variations of regenerants. Moreover, analyzing plant …
Morphological Characterization, Pollen Viability, And Germinability Evaluation Of Two Plumeria Species In The University Of The Philippines Los Baños, Darel Kenth S. Antesco, Maria Charito E. Balladares, Alangelico O. San Pascual, Pablito M. Magdalita, Fernando C. Sanchez Jr
Morphological Characterization, Pollen Viability, And Germinability Evaluation Of Two Plumeria Species In The University Of The Philippines Los Baños, Darel Kenth S. Antesco, Maria Charito E. Balladares, Alangelico O. San Pascual, Pablito M. Magdalita, Fernando C. Sanchez Jr
The Philippine Agricultural Scientist
The genus Plumeria, locally known as kalachuci, is a popular ornamental tree belonging to the Apocynaceae family. The University of the Philippines Los Baños (UPLB) maintains a collection of Plumeria trees scattered across its campus; however, the origin of these trees remains unclear due to a lack of proper documentation. This study conducted morphological characterization and pollen analysis to provide information for further research. Results revealed the presence of 2 Plumeria species in the campus. P. obtusa has a linear leaf shape with acute tips and revolute margins while P. rubra has a lanceolate leaf shape. Additionally, P. …
Antibiotic Resistome, Mobile Genetic Elements, And Virulence Profile Of Multidrug-Resistant Enterobacteriaceae Isolated From Healthy Philippine Native Chicken As Revealed By Analysis Of Whole Genome Sequence, Alvin P. Jimenez, Grignard James R. Buday, Rina B. Opulencia
Antibiotic Resistome, Mobile Genetic Elements, And Virulence Profile Of Multidrug-Resistant Enterobacteriaceae Isolated From Healthy Philippine Native Chicken As Revealed By Analysis Of Whole Genome Sequence, Alvin P. Jimenez, Grignard James R. Buday, Rina B. Opulencia
The Philippine Agricultural Scientist
Gram-negative bacteria, particularly Enterobacteriaceae, acquire antibiotic resistance via horizontal transmission of resistance genes in poultry farms that give rise to multidrug-resistant (MDR) strains. The presence of MDR strains that are potentially pathogenic and zoonotic poses a concern to animal and human health. The aims of this study were to isolate MDR, potentially pathogenic Enterobacteriaceae from Philippine native chicken (PNC) and to analyze the genome of a representative MDR isolate to gain insights into the mechanisms, acquisition, and transmissibility of antibiotic resistance genes (ARGs). Cloacal swabs from PNC were used to isolate putative Salmonella that were then tested for antimicrobial …
Production Objectives And Trait Preferences Of Smallholder Farmers In The Philippines: Implications For Designing Breeding Schemes Utilizing Indigenous Swine Genetics, Joy Banayo, Kathlyn Louise Manese, Kaito Furusho, Madeline Kingan, Justine Ayomen, Marcelino Saliw-An, Karina Marie Nicolas, Kayvin Joel Petipit, Dorothy Pagbilao, Vea Roven Arellano, Rene Santiago, Flomella Caguicla, Arnolfo Monleon, Giselle Perlas, Giselle Perlas, Rea Palma Ortego, Sharon Singzon, Agapita Salces, Takahiro Yamagata
Production Objectives And Trait Preferences Of Smallholder Farmers In The Philippines: Implications For Designing Breeding Schemes Utilizing Indigenous Swine Genetics, Joy Banayo, Kathlyn Louise Manese, Kaito Furusho, Madeline Kingan, Justine Ayomen, Marcelino Saliw-An, Karina Marie Nicolas, Kayvin Joel Petipit, Dorothy Pagbilao, Vea Roven Arellano, Rene Santiago, Flomella Caguicla, Arnolfo Monleon, Giselle Perlas, Giselle Perlas, Rea Palma Ortego, Sharon Singzon, Agapita Salces, Takahiro Yamagata
The Philippine Agricultural Scientist
Profit maximization is not the only production objective in farming. For smallholder farms worldwide, family sustenance often takes priority, with production being viewed more as savings-oriented than market-oriented. Furthermore, market pressures can lead to the mismanagement of indigenous livestock resources, increasing their risk of genetic erosion. To ensure the long-term conservation of the Philippine native pig, this study aimed to determine the various types of native pig farmers and define the intervention strategies for each type. A total of 432 native pig farmers representing seven provinces in the Philippines (Benguet, Kalinga, Isabela, Nueva Vizcaya, Quezon, Marinduque, and Eastern Samar; n …
Root Plasticity Of Selected Corn (Zea Mays L.) Varieties Grown For Food And Forage In Response To Fertigroe®-N And Urea, Vanica Apostol, Edna Aguilar, Lilia Fernando, Lourdes Edano Ma., Tonette Laude, Eureka Teresa Ocampo
Root Plasticity Of Selected Corn (Zea Mays L.) Varieties Grown For Food And Forage In Response To Fertigroe®-N And Urea, Vanica Apostol, Edna Aguilar, Lilia Fernando, Lourdes Edano Ma., Tonette Laude, Eureka Teresa Ocampo
The Philippine Agricultural Scientist
Root plasticity, the capacity of a corn root system to modify its growth and development in response to varying environmental conditions, is a crucial adaptability trait for optimizing water and nutrient uptake, especially in a challenging environments. Understanding root plasticity provides insights into the pattern basis of crop adaptation, contributing to the development of improved corn varieties and agricultural practices that optimize the production of both ear shoots and forage. This study assessed root traits and other morphological-physiological responses of three corn varieties (NK8840, IPB Var 6, and Pearl Sweet) grown for food (ear shoots) and forage (baby corn fodder) …
Genetic Diversity Analysis Of Greater Yam (Dioscorea Alata L.) Collections Using Tuber Morphology And Simple Sequence Repeats (Ssr) Markers, Junelyn Ravelo, Lara Jaaziel Batalon, Antonio Lalusin
Genetic Diversity Analysis Of Greater Yam (Dioscorea Alata L.) Collections Using Tuber Morphology And Simple Sequence Repeats (Ssr) Markers, Junelyn Ravelo, Lara Jaaziel Batalon, Antonio Lalusin
The Philippine Agricultural Scientist
The diversity of 148 greater yam (Dioscorea alata L.) accessions from the collections of the National Plant Genetics Resources Laboratory (NPGRL), Visayas State University (VSU), and the Institute of Crop Science - University of the Philippines Los Baños (ICROPS-UPLB) was evaluated using 54 Dioscorea-based SSR markers. Out of 54 SSR markers, polymorphic bands were amplified in 50 SSR markers with Polymorphic Information Content (PIC) values ranging from 0.65 to 0.89. Subsequent cluster analysis generated nine distinct clusters with a Jaccard’s distance index of 0.85, implying 85% dissimilarity among the greater yam accessions. Each formed cluster from the analysis comprised …
Genetic Approaches To Improving Coffee Resistance Against Diseases And Pests In The Philippines [Review Paper], Nick Rainier Santos, Ernelea Cao, Daisy May Santos
Genetic Approaches To Improving Coffee Resistance Against Diseases And Pests In The Philippines [Review Paper], Nick Rainier Santos, Ernelea Cao, Daisy May Santos
The Philippine Agricultural Scientist
There is a huge demand for coffee in the Philippines but local supply has a long way to go at catching up. One way to aid local farmers is to develop coffee varieties with combined desirable traits including natural resistance against various diseases and insect pests. This can increase product output and reduce costs on chemical control that can, in turn, potentially increase their profits. This paper reviews and gives an overview of the genetic research on coffee toward improving plant defense. The most prominent diseases and pests plaguing plantations worldwide are noted. From studies using classical tools such as …
Agrobacterium-Mediated Genetic Transformation And Plant Regeneration From Cotyledons In Philippine Eggplant (Solanum Melongena L.) Acc. ‘Ph 11424’, Mark Gabriel Sagarbarria, John Albert Caraan, Patrick Lipio, Ian Bien Oloc-Oloc, Kazuo Watanabe, Desiree Hautea
Agrobacterium-Mediated Genetic Transformation And Plant Regeneration From Cotyledons In Philippine Eggplant (Solanum Melongena L.) Acc. ‘Ph 11424’, Mark Gabriel Sagarbarria, John Albert Caraan, Patrick Lipio, Ian Bien Oloc-Oloc, Kazuo Watanabe, Desiree Hautea
The Philippine Agricultural Scientist
Eggplant (Solanum melongena L.) is one of the most important vegetables grown and consumed in the Philippines hence, continuous breeding programs are vital to maintain the supply of this economically important crop. This study demonstrates the first successful Agrobacterium-mediated genetic transformation and plant regeneration of the Philippine eggplant cultivar ‘PH 11424’, also known as ‘Mistisa’. Cotyledons from 2-wk-old seedlings were used as explants, which were transformed with disarmed Agrobacterium tumefaciens strain LBA4404 harboring a binary vector for CRISPR/Cas9 expression and hygromycin phosphotransferase (HPT), an antibiotic selection marker. Growth of shoot primordia from the agro-infected explants was observed during selective culture …
Protective Responses Of The Intestinal Epithelial Cell Line Ht-29 Cells Exposed To Dephosphorylated Salmonella Flagellin [Research Note], Heyeon Baik, Jaiesoon Cho
Protective Responses Of The Intestinal Epithelial Cell Line Ht-29 Cells Exposed To Dephosphorylated Salmonella Flagellin [Research Note], Heyeon Baik, Jaiesoon Cho
The Philippine Agricultural Scientist
This study aimed to describe the effects of Salmonella Typhimurium flagellin (SFL) dephosphorylated by sweet potato purple acid phosphatase (PAP) on the protective responses of the intestinal epithelial cell line HT-29 cells. The enzyme was reported to display a broad substrate specificity for various organic phosphorylated conjugates and phosphoproteins. Dephosphorylation of SFL by sweet potato PAP decreased to 35% in the presence of 0.05 mM vanadate as compared with the negative control (p < 0.05). Intact SFL and the SFL treated with sweet potato PAP did not remarkably induce the activation of caspase-3 in HT-29 cells at all the tested levels of the substrate. Intact SFL maximally induced the release of IL (interleukin)-8 in HT-29 cells at 1000 ng/mL (p < 0.05). However, the SFL treated with the enzyme inhibited the release of IL-8 at over 100 ng/mL of the substrate as compared with intact SFL, resulting in an approximately 8-fold decrease even at 1000 ng/mL (p < 0.05). The SFL treated with the enzyme decreased the activation of the total ERK1/2 in the cells to 1.9 and 1.7–fold at 10 and 1000 ng/mL of the substrate, respectively, as compared with intact SFL (p < 0.05). In conclusion, sweet potato PAP could be a promising tool for controlling excessive inflammation during Salmonella infection in animal husbandry, and the enzyme could be a safe alternative that can overcome the drawbacks of chemotherapy.
Molecular Mimicry Of Noda Genes By An N-Acetylmuramoyl-L-Alanine Amidases Gene In A Nodule Forming Staphylococcus Cohnii [Research Note], Aberathne Herath Mudiyanselage Nadeeshani Ruwandika Aberathne, Wedage Methsala Madurangi Wedage, Dilantha Gunawardana
Molecular Mimicry Of Noda Genes By An N-Acetylmuramoyl-L-Alanine Amidases Gene In A Nodule Forming Staphylococcus Cohnii [Research Note], Aberathne Herath Mudiyanselage Nadeeshani Ruwandika Aberathne, Wedage Methsala Madurangi Wedage, Dilantha Gunawardana
The Philippine Agricultural Scientist
A rare, nodule-forming, Gram-positive bacterium (Sub4) was isolated from the forage and cover crop Pueraria phaseoloides, which aligned at 99.26% sequence identity to a partial 16S rDNA sequence of Staphylococcus cohnii sp. This was the first record of a strain/species of Staphylococcus capable of independent, unassisted nodule formation in a legume host. When a nodA gene was sought by PCR using a pair of gene-specific primers synthesized using a related (Firmicutes) Paenibacillus sp. nodA gene, the reaction yielded a PCR product of similar size but a distinct identity. The resulting ~400bp PCR product coded for a likely N-acetylmuramoyl-L-alanine amidase …
Validation Of Newly Designed Ssr Markers For Eight Rice (Oryza Sativa L.) Genotypes With Variable Heat Tolerance Responses Based On Agromorphic Data And Pollen Fertility Analysis [Research Note], Monaliza B. Magat, Norvie L. Manigbas, Jessica D. Rey
Validation Of Newly Designed Ssr Markers For Eight Rice (Oryza Sativa L.) Genotypes With Variable Heat Tolerance Responses Based On Agromorphic Data And Pollen Fertility Analysis [Research Note], Monaliza B. Magat, Norvie L. Manigbas, Jessica D. Rey
The Philippine Agricultural Scientist
Rice is one of the most valuable staple food crops in the world. However, several challenges greatly affect production, one of which is the threat imposed by heat stress. To address this, researchers are developing varieties that are heat stress tolerant with genetic markers aid. In this study, eight rice genotypes, namely Dular, Nagina 22, NSIC Rc 222, Milyang 23, EL15, EL92, EL85, and IR52 were observed for agromorphic data, which included plant height, panicle length, filled and unfilled grains, and grain yield. Flower samples were collected to determine the effect of heat stress on pollen fertility. Molecular markers were …
Determination Of Some Seed Characteristics Of False Flax (Camelina Sativa L. Crantz) Genotypes Grown Under Semi-Temperate Conditions, İlhan Subaşı, Yusuf Arslan, Tamer Eryigit, Vahdettin Çiftçi, Mahmut Çamlıca
Determination Of Some Seed Characteristics Of False Flax (Camelina Sativa L. Crantz) Genotypes Grown Under Semi-Temperate Conditions, İlhan Subaşı, Yusuf Arslan, Tamer Eryigit, Vahdettin Çiftçi, Mahmut Çamlıca
The Philippine Agricultural Scientist
Camelina is an alternative low-input oil plant grown in marginal areas in Turkey. This study aimed to determine some quality characteristics of 39 camellia genotypes in ecological conditions in Bolu, Turkey. The field experiments were carried out in an augmented design with randomized blocks. In this work, some important properties such as thousand seed weight, oil content, fatty acid composition, and protein ratio were investigated. Results showed that mean data for thousand seed weight, oil ratio, and protein ratio were 0.63 – 1.5 g, 26.69 – 39.17%, and 21.81 – 29.81%, respectively. Considering the oil content of the genotypes used …
Determining Some Quality Properties Of Oat Genotypes Collected From The Middle And West Black Sea Region, Zeki Mut, Hasan Akay, Ozge Doganay Erbas Kose, Ismail Sezer
Determining Some Quality Properties Of Oat Genotypes Collected From The Middle And West Black Sea Region, Zeki Mut, Hasan Akay, Ozge Doganay Erbas Kose, Ismail Sezer
The Philippine Agricultural Scientist
Healthy foodstuffs, including functional foods, are the key component of a healthy and prosperous life. Oat grains are rich in protein, soluble dietary fiber, β-glucan, starch, oil, some vitamins and minerals, and thus accepted as a healthy foodstuff. This study was conducted to determine physical and chemical quality traits of many different oat landraces/cultivars. In this study, 251 local oat genotypes, collected from 10 provinces of Western and Middle Black Sea Regions of Turkey and four standard oat cultivars were grown over the experimental fields of the Agricultural Application and Research Center at Samsun Ondokuz Mayıs University for two years …
Epinasty Accompanies Potassium Nitrate-Induced Flower Bud Break In 'Carabao' Mango [Research Note], Ulysses Besas, Calixto Protacio
Epinasty Accompanies Potassium Nitrate-Induced Flower Bud Break In 'Carabao' Mango [Research Note], Ulysses Besas, Calixto Protacio
The Philippine Agricultural Scientist
The hypothesis that potassium nitrate application promotes epinasty in ‘Carabao’ mango through increased ethylene production was investigated. The degree of drooping, apical primordium growth as affected by shoot ethylene and levels after potassium nitrate spraying were monitored. Evidence presented established for the first time in ‘Carabao’ mango that epinasty occurred just before flower emergence, coinciding with visible bud break, and extended up to 18 days, marking floral anthesis. The possible reasons could be the higher levels of ethylene at 0.31% and earlier increase of ethylene in the shoot apex at four hours and apical primordium growth. Increased ethylene levels at …
Efficacy Study Of Selected Maize (Zea Mays L.) Genotypes Against The Asian Corn Borer, Ostrinia Furnacalis (Guenée), Yoryncita Marfori, Merdelyn Caasi-Lit, Tonette Laude
Efficacy Study Of Selected Maize (Zea Mays L.) Genotypes Against The Asian Corn Borer, Ostrinia Furnacalis (Guenée), Yoryncita Marfori, Merdelyn Caasi-Lit, Tonette Laude
The Philippine Agricultural Scientist
Laboratory and screen house studies were conducted to assess the efficacy of selected twenty-six maize varieties against Asian corn borer (ACB), Ostrinia furnacalis (Guenée). Hierarchical tree based on resistance parameters clearly revealed that the twenty-six maize varieties are best classified into three groups, viz. resistant, intermediate, and susceptible. In laboratory test, the varieties RCS2, RCS4, RCS5, 'IPB Var13', Bt2, Bt3, and Bt4 were consistently identified as highly resistant. Further examinations in stalk feeding assay showed that the resistant varieties had sharp decline in larval survival (LS) on the 2nd to the 5th day of larval feeding in …
Spectral Lights Based Treatment Enhanced Biomass Accumulation And Secondary Metabolites Production In Callus Culture Of Citrus Reticulata, Faiza Anum, Naveed Iqbal Raja, Tahira Sultana, Abeer Kazmi, Amir Ali, Bushra Qayyum, Anza Afzal, Aneela Nijibat, Zia-Ur-Rehman Mashwani, Abeer Kazmi
Spectral Lights Based Treatment Enhanced Biomass Accumulation And Secondary Metabolites Production In Callus Culture Of Citrus Reticulata, Faiza Anum, Naveed Iqbal Raja, Tahira Sultana, Abeer Kazmi, Amir Ali, Bushra Qayyum, Anza Afzal, Aneela Nijibat, Zia-Ur-Rehman Mashwani, Abeer Kazmi
The Philippine Agricultural Scientist
Citrus reticulata is a common fruit, belongs to the family Rutaceae. It contains terpenes, phenolics, flavonoids, and vitamin C in conjugation with sugars, amino acids, minerals, organic acids and other biologically important compounds. Application of light elicitation strategies in in vitro plant cultures is acquiring more interest for their potential in feasible biomass accumulation and therapeutically important secondary metabolites production. The present study was designed to investigate the effects of different monochromatic lights (white, red, blue, green, and yellow) on the proliferation and secondary metabolites accumulation in callus cultures of Citrus reticulata raised in response to treatment with AgNPs and …
Genetic Diversity And Population Structure Of Sugarcane (Saccharum Officinarum L.) Collected From Seven Philippine Regions For Use As Breeding Stocks For Crop Improvement, Jan Leandro Acedera, Jhun Laurence Rasco, Jemimah Banganan, Antonio Lalusin
Genetic Diversity And Population Structure Of Sugarcane (Saccharum Officinarum L.) Collected From Seven Philippine Regions For Use As Breeding Stocks For Crop Improvement, Jan Leandro Acedera, Jhun Laurence Rasco, Jemimah Banganan, Antonio Lalusin
The Philippine Agricultural Scientist
Selection of parents for hybridization relies on the information on their genetic relationship and diversity which are essential in any breeding program. This study aimed to estimate the extent of genetic diversity and population structure of 76 sugarcane accessions from seven regions in the Philippines using 57 morphological characters and 50 microsatellite markers. The sugarcane collections exhibited moderate to high diversity with mean of H’ = 0.72 for qualitative and H’ = 0.75 for quantitative morphological characters, respectively. This is corroborated by the analysis of variance (ANOVA) of agronomic parameters, except for stalk length. Unweighted Pair Group Method with Arithmetic …
Weedy Rice Conserved Ex Situ Characterized Using Morphological And Simple Sequence Repeat (Ssr) Markers, Rubylyn Mijan Infante, Maria Elizabeth B. Naredo, Merlyn S. Mendioro
Weedy Rice Conserved Ex Situ Characterized Using Morphological And Simple Sequence Repeat (Ssr) Markers, Rubylyn Mijan Infante, Maria Elizabeth B. Naredo, Merlyn S. Mendioro
The Philippine Agricultural Scientist
A clear understanding of genetic diversity in weedy rice is important in improving protocols for its conservation, ex situ. To this end, Asian collections of weedy rice together with accessions of the wild AA genome species Oryza nivara Sharma et Shastry and Oryza rufipogon Griffith, and the cultivated species, Oryza sativa L. were characterized using 16 qualitative morphological traits and 18 SSR markers. The Shannon-Weaver index (H’) revealed higher diversity in the weedy (mean H’ = 0.76) and wild O. nivara (mean H’ = 0.79) and O. rufipogon samples (mean H’ = 0.69) compared to the cultivated …
Grain Yield Variations In Rice Genotypes Under Different Growing Environments In The Philippines, Kim Nyka C. Perdiguerra, Shiro Mitsuya, Akira Yamauchi, Felino P. Lansigan, Maria Victoria O. Espaldon, Jose E. Hernandez, Pompe C. Sta. Cruz
Grain Yield Variations In Rice Genotypes Under Different Growing Environments In The Philippines, Kim Nyka C. Perdiguerra, Shiro Mitsuya, Akira Yamauchi, Felino P. Lansigan, Maria Victoria O. Espaldon, Jose E. Hernandez, Pompe C. Sta. Cruz
The Philippine Agricultural Scientist
Grain yield variations in PSB Rc18, NSIC Rc222 (inbreds) and NSIC Rc202H (hybrid) were determined across growing environments as a function of cropping seasons and locations in rice producing areas in the Philippines. Contribution of location to variation in grain yield is 61.0%, while 12.7% for the season, and 6.1% for the genotype and this must be due location by season by genotype interactions. Dry season cropping in Nueva Ecija produced the highest mean grain yield. On the other hand, wet season cropping in Davao del Sur produced the least mean grain yield. The genotypes differed in their response to …
Genetic Variability In Fusarium Oxysporum F. Sp. Vasinfectum Causing Damping-Off Of Cotton (Gossypium Barbadense L.) Seedlings [Research Note], Reda Helmy Sammour, Salah Abdel Moemen, Eman Elagamy
Genetic Variability In Fusarium Oxysporum F. Sp. Vasinfectum Causing Damping-Off Of Cotton (Gossypium Barbadense L.) Seedlings [Research Note], Reda Helmy Sammour, Salah Abdel Moemen, Eman Elagamy
The Philippine Agricultural Scientist
The genetic variability of Fusarium oxysporum f. sp. vasinfectum isolates from Gossypium barbadense L. seedlings were assessed using pathogenicity, mycelial compatibility, and RAPD analyses. The results showed that the disease incidence percentages and compatibility percentages considerably varied, extending from 33.2 to 86.6 % and 6 to 60 %, respectively in North Egypt and from 33.3 to 66.6 % and 0.0 to 20% in South Egypt. It also showed that the isolates ISO-8 and ISO-19 had the lowest disease incidence (33.2%) as compared with isolate ISO-5 (86.6%). Results of the mycelial compatibility had a reversible relationship with disease incidence percentage. Cluster …
Morphological And Microsatellite-Based Molecular Characterization Of Locally Collected Sugarcane (Saccharum Officinarum L.), Carl F. Libayao, Carmina C. Manuel Ma., Eureka Teresa M. Ocampo, Antonio G. Lalusin
Morphological And Microsatellite-Based Molecular Characterization Of Locally Collected Sugarcane (Saccharum Officinarum L.), Carl F. Libayao, Carmina C. Manuel Ma., Eureka Teresa M. Ocampo, Antonio G. Lalusin
The Philippine Agricultural Scientist
Fifty-three sugarcane accessions from Aklan, Iloilo, Cagayan, Nueva Vizcaya, Isabela, and Bohol were characterized using forty-two morphometric parameters and forty microsatellite markers. Twenty-six morphological characters using the Shannon-Weaver diversity index showed high variability (H’ = > 0.76) and were able to characterize the fifty-three sugarcane accessions. Cluster analysis of morphological traits based on sequential agglomerative hierarchical test and Euclidean distance revealed two groupings at 0.3 coefficient of dissimilarity. The clustering of some accessions was irrespective of their geographical origin indicating a high degree of phenotypic similarity in some accessions. Out of the forty microsatellite markers, twenty-five (63%) have a PIC value …
Phenotypic Characters And Expression Of Cytokinin Oxidase 2 (Osckx2) Gene In Rice Genotypes With Enhanced Grain Filling Traits, Cielo Luz C. Mondejar, Maria Genaleen Q. Diaz, Teresita H. Borromeo, Tonette P. Laude, Arlen A. Dela Cruz, Roel Rodriguez Suralta
Phenotypic Characters And Expression Of Cytokinin Oxidase 2 (Osckx2) Gene In Rice Genotypes With Enhanced Grain Filling Traits, Cielo Luz C. Mondejar, Maria Genaleen Q. Diaz, Teresita H. Borromeo, Tonette P. Laude, Arlen A. Dela Cruz, Roel Rodriguez Suralta
The Philippine Agricultural Scientist
Poor grain filling is still a major problem in rice production. Recent attention in crop varietal improvement is focused on heritable delayed foliar senescence, enhanced root system, and fast-synchronous grain filling pattern. Nine genotypes were characterized for these three enhanced grain-filling traits to determine the role of these traits on grain yield. Based on heritable delayed foliar senescence, NSIC Rc436 and NSIC Rc512 were characterized as Type A, while PSB Rc18 and NSIC Rc514 as Type B. These genotypes had a significantly higher yield than IR64, owing to their high spikelets number. Data suggested a positive correlation between the delayed …
16s Rrna Gene Sequence Analysis Of Acetic And Lactic Acid Bacteria Isolated From Philippine Sugarcane Wine (Basi) [Research Note], John Russel G. Sevilla, Michael Angelo S. Esteban, Honey Bhabes R. Iñigo, Audrey May V. Orillaza, Baby Richard R. Navarro
16s Rrna Gene Sequence Analysis Of Acetic And Lactic Acid Bacteria Isolated From Philippine Sugarcane Wine (Basi) [Research Note], John Russel G. Sevilla, Michael Angelo S. Esteban, Honey Bhabes R. Iñigo, Audrey May V. Orillaza, Baby Richard R. Navarro
The Philippine Agricultural Scientist
Basi, a traditional sugarcane wine of the Philippines, was studied. Here, we used molecular- and cultural-based methods to isolate, identify and characterize acid-producing bacteria, specifically acetic and lactic acid bacteria, from basi. Acid producers were focused on owing to the rapid spoilage of basi via acidification with air exposure. Two strains each of acetic and lactic acid bacteria were isolated. DNA was extracted from these isolates. PCR-amplified DNA products were subjected to 16S rRNA gene sequencing. The sequences of the isolates were then aligned with BLAST database sequences and found to have high similarities to Acetobacter malorum (99%), …