Genetics and Genomics Commons^™

A Temperature-Sensitive Mutant Of Escherichia Coli Affected In The Alpha Subunit Of Rna Polymerase, Majid Mehrpouyan

Electronic Theses and Dissertations

A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase has been investigated. Gene mapping and complementation experiments placed the mutation to temperature-sensitivity within the alpha operon at 72 min on the bacterial chromosome. The rate of RNA synthesis in vivo and the accumulation of ribosomal RNA were significantly reduced in the mutant at 44$\sp\circ$C. The thermostability at 44$\sp\circ$C of the purified holoenzyme from mutant cells was about 20% of that of the normal enzyme. Assays with T7 DNA as a template showed that the fraction of active enzyme competent for transcription was reduced as a …

The Australian Lupin Collection - Passport Data For Wild And Semi-Domesticated Accessions Introduced Into Australia To 1990, J. C. Clements, W. A. Cowling

Grain and other field crops published reports

This report contains passport (including collection site) data and Commonwealth Plant Introduction (CPI) numbers for all wild and semi-domesticated Lupinus accessions introduced into Australia to 1990 (a total of 1071 accessions with CPI numbers between 10650 and 122692). Scientists or plant breeders with an interest in the characteristics of wild lupins from certain natural environments, such as high soil pH or low rainfall, will find this information invaluable when they select accessions for their experiments. The majority of the accessions were introduced by Western Australian scientists following collecting missions in the Mediterranean region, and the remainder were donated from institutions …

Application Of Dna Fingerprints For Cell-Line Individualization, Dennis A. Gilbert, Yvonne Reid, Mitchell H. Gail, David Pee, Christine White, Robert J. Hay, Stephen J. O'Brien

Biology Faculty Articles

DNA fingerprints of 46 human cell lines were derived using minisatellite probes for hypervariable genetic loci. The incidence of 121 HaeIII DNA fragments among 33 cell lines derived from unrelated individuals was used to estimate allelic and genotypic frequencies for each fragment and for composite individual DNA fingerprints. We present a quantitative estimate of the extent of genetic difference between individuals, an estimate based on the percentage of restriction fragments at which they differ. The average percent difference (APD) among pairwise combinations from the population of 33 unrelated cell lines was 76.9%, compared with the APD in band sharing …

Dna Recombination And Natural Selection Pressure Sustain Genetic Sequence Diversity Of The Feline Mhc Class I Genes, Naoya Yuhki, Stephen J. O'Brien

Biology Faculty Articles

Sequence comparisons of seven distinct MHC class I cDNA clones revealed that feline class I molecules have a remarkable similarity to human HLA genes in their organization of functional domains as well as in the nonrandom partitioning of genetic variability according to the functional constraints ascribed to different regions of the MHC molecule. The distribution of the pattern of sequence polymorphism in the cat as compared with genetic diversity of human and mouse class I genes provides evidence for four coordinate factors that contribute to the origin and sustenance of abundant allele diversity that characterizes the MHC in the species. …

The Use Of Synthetic Oligodeoxyribonucleotides In Direct Analysis Of Single Nucleotide Variations And Inhibition Of Eukaryotic Gene Expression, Daniel Y. Wu

Loma Linda University Electronic Theses, Dissertations & Projects

The present thesis work contributes to two areas of molecular genetics, namely the development of methods for identifying single nucleotides and the understanding of the mechanism by which antisense oligonucleotides inhibit eukaryotic gene expression. Three single nucleotide detection methods are presented. The first method utilizes hybridization with synthetic oligonucleotide probes to detect single or multiple nucleotide differences between alleles in genomic DNA without electrophoretic separation. Total genomic DNA (either digested or undigested with restriction endonucleases) was immobilized in depressions in an agarose gel (in situ dots) and hybridized with radiolabeled, allele-specific oligonucleotide probes. We show that this method can be …

Effects Of Chemical Aneuploidogens On Taxol Purified Drosophila And Mouse Brain Microtubules Polymerization And Depolymerization In Vitro, Anil Sehgal

Biological Sciences Theses & Dissertations

The effects of aneuploidogens (aneuploidy causing agents) on taxol-purified microtubules from Drosophila and mouse brain in vitro were studied by using a spectrophotometric assay and electron microscopy. Colchicine, acetonitrile, propionitrile, acrylonitrile, dimethyl sulfoxide (DMSO), griseofulvin and cadmium chloride inhibited microtubule polymerization whereas methoxyethyl acetate (MEA) and methyl mercuric chloride (MMC) did not. All aneuploidogens tested (at 50mM) resulted in reduced rate of elongation of mouse brain microtubules. MMC, cadmium chloride and DMSO resulted in increased rates of Drosophila microtubule elongation whereas the rest of the drugs resulted in decreases. The in vitro results from Drosophila correlate well with the previously …

Bulletin No 4187 - Choosing Commercial Beef Bulls, David Barker

Bulletins 4000 -

This bulletin provides a method of placing realistic differences in value on young bulls offered for sale.

The prices paid for young bulls are often based on superficial appearance alone, and this has important financial implications for the purchaser. Different characteristics have either large or small effects on the net value of progeny produced. They are dependent on such factors as: the variability of different characteristics in cattle; differences in reliability of the assessment; the extent to which the characteristic is passed on to the progeny; the effect of the characteristic on realized net value of the progeny; the effect …

Characterization Of Hsd::Mudx128 Operon Fusion Mutant Of Escherichia Coli K-12, Marjorie Ann T. Reyno

Loma Linda University Electronic Theses, Dissertations & Projects

The study of the regulation of gene expression in complex genes can be facilitated by the use of operon fusions which place a well characterized lacZ gene under the control of a promoter of interest. An hsd::MudX128 operon fusion mutant of E. coli K-12 isolated by Prakash (1986) was observed to have a high β-galactosidase activity (1000 units), ten to twenty times higher than other hsd::MudX mutants. This β-galactosidase activity was suppressed to a very low level (25 units) by the introduction of an F’ plasmid, F'101, which carries the 98 min to 2 min region of the …

Prevalence And Implications Of Feline Coronavirus Infections Of Captive And Free-Ranging Cheetahs (Acinonyx Jubatus), J. L. Heeney, J. F. Evermann, A. J. Mckeirnan, L. Marker-Kraus, M. E. Roelke, M. Bush, D. E. Wildt, D. G. Meltzer, L. Colly, J. Lukas, V. J. Manton, T. Caro, Stephen J. O'Brien

Biology Faculty Articles

The extent and progression of exposure to feline infectious peritonitis (FIP) virus in the cheetah, Acinonyx jubatus, was monitored by a world-wide serological survey with indirect fluorescent antibody titers to coronavirus. The indirect fluorescent antibody assay was validated by Western blots, which showed that all indirect fluorescent antibody-positive cheetah sera detected both domestic cat and cheetah coronavirus structural proteins. There was a poor correlation between indirect fluorescent antibody results and the presence of coronaviruslike particles in cheetah feces, suggesting that electron microscopic detection of shed particles may not be an easily interpreted diagnostic parameter for FIP disease. Low, but …

Differences Between Merino Strains And Studs, Roger Lewer

Journal of the Department of Agriculture, Western Australia, Series 4

When commercial wool growers select Merino rams they often use the following pattern: the buyers first consider the main strains of Merinos in Western Australia - Bungaree, Collinsville and Peppin. They then study the 550 studs and numerous non-stud ram breeders in this State. Having chosen a breeder, they examine the rams on offer and buy their annual requirements from among these. Until recently, no objective information has been available on the differences between studs and strains in Western Australia's agricultural areas. However, as part of a major study at the Department of Agriculture's Great Southern Agricultural Research Institute, these …

Molecular And Cytogenetic Characterization Of De Novo Acrocentric Rearrangements In Humans, Lisa Gail Shaffer

Theses and Dissertations

I have studied 26 children who have a de novo rearrangement of the acrocentric chromosomes in order to understand the formation of these aberrations. The families include '25 probands ascertained for Robertsonian-type translocations, 13 between nonhomologous chromosomes and 12 between homologs, and one rea(21;21)(q22;q22). The parental origins of the de novo rearrangements were determined in 26/26 families using QFQ and NOR variants and/or RFLP analyses. While there was no overall difference in the sex distribution of the parents of origin, there were more maternally derived nonhomologous ("true" Robertsonian) translocations (8 mat: 5 pat) and more paternally derived homologous rearrangements (4 …

Macrophages But Not B Cells From Aged Mice Are Defective In Stimulating Autoreactive T Cells In Vitro, Aruna Seth, Mitzi Nagarkatti, Prakash S. Nagarkatti, Subbarao Bondada, Venkatachalam Udhayakumar

Microbiology, Immunology, and Molecular Genetics Faculty Publications

In the present study the effect of aging on the capacity of Ia⁺ cells to stimulate autoreactive T cells in the syngeneic mixed lymphocyte reaction (SMLR) was investigated. Using young CD4⁺ T cells as responders, it was observed that unseparated whole spleen cells from aged mice had normal stimulatory activity comparable to that of young spleen cells. Interestingly, however, when purified splenic adherant cells (SAC) enriched for macrophages or splenic B cells were used as stimulators, aged SAC but not aged B cells were found to be defective in stimulating autoreactive T cells. This defect in aged SAC …

Phenotypic And Functional Properties Of B Lymphocytes From Aged Mice, Subbarao Bondada, Joan Morris, Richard J. Kryscio

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Phenotypic and functional properties of B lymphocytes from individual young and old mice of different inbred strains were studied. B lymphocyte subpopulation defined by the ratios of the densities of cell surface IgM and IgD were found to be altered with age. However, such alterations in B cell subsets were found only in 30-40% of the old mice. B cell mitogenic responses to anti-μ and anti-Lyb2 antibodies were decreased in a majority of DBA/2 mice. Proliferative responses to LPS and antiμ were reduced only in a minority of CBA/Ca mice but there was a very good correlation in the responsiveness …

Freshwater Fishes Of Shipstern Nature Reserve, Caspar F. A. Bijleveld

Physiology Faculty Research Publications

A survey of fishes in and around Shipstern Nature Reserve in Northern Belize, Central America, was carried out over a period of eight months, from February to September 1990. Its purpose was to establish a preliminary list of the fishes of Shipstern Nature Reserve, including descriptions, distribution and collecting data and additionally to describe some of the habitats in which the fishes were found. This study focuses on fishes considered to be freshwater species. These occurred for the greater part in brackish habitats. Nevertheless, a few fish considered as marine species were caught in the waters of the reserve and …

Preliminary Agronomic Evaluation And Characterization Of The Genetic Resources Of Trifolium And Ornithopus Species., R. Snowball

Experimental Summaries - Plant Research

Trial 91MD13

Location: Medina Vegetable Research Station

To evaluate and characterize new and old collections of Trifolium and Ornithopus species.

To regenerate lines to provide sufficient seed of high quality for long term storage in the Australian Trifolium Genetic Resource Centre (ATGRC).

Barry Morton Gough's Review Of Robert Langdon's The Lost Caravel Re-Explored, Barry Morton Gough

Pacific Studies

No abstract provided.

Can Genetically Engineered Crops Become Weeds?, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

There are significant differences if the distribution of weedy characteristics among weeds, normal plants, and crops. The world’s most serious weeds possess on the average 10 or 11 of these characters, a random collection of British plants have an average seven of the traits, and crop plants only five. For the average crop to become as “weedy” as the average weed, it would need to acquire five weedy traits. Even using the unlikely assumption that those traits are single loci in which a dominant mutation would provide the weedy character, this would require the simultaneous acquisition of five gene substitutions. …

Micronuclear Genome Organization In Euplotes Crassus: A Transposonlike Element Is Removed During Macronuclear Development, Scott Everet Baird, Gina M. Fino, Susan L. Tausta, Lawrence A. Klobutcher

Biological Sciences Faculty Publications

After mating, hypotrichous ciliated protozoa transform a set of their micronuclear chromosomes into thousands of short, linear DNA molecules that form the macronuclear genome. To examine micronuclear genome organization in the hypotrich Euplotes crassus, we have analyzed two cloned segments of micronuclear DNA as well as the macronuclear DNA molecules that are derived from them. E. crassus was found to display a number of features characteristic of other hypotrich genomes, including (i) clustering and close spacing of the precursors of macronuclear DNA molecules, (ii) the frequent occurrence of internal eliminated sequences within macronuclear precursors, (iii) overlapping macronuclear precursors, (iv) lack …

Chromosomal Localization Of Nucleic Acid-Binding Proteins By Affinity Mapping: Assignment Of The Ire-Binding Protein Gene To Human Chromosome 9, Matthias W. Hentze, Hector Seuanez, Stephen J. O'Brien, Joe B. Harford, Richard D. Klausner

Biology Faculty Articles

Three human mRNAs are regulated post-transcriptionally by iron via iron-responsive elements (IREs) contained in each mRNA. A cytoplasmic protein (IRE-BP) binds to these cis-acting elements and mediates the translational regulation of ferritin H- and L-chain mRNA and the iron-dependent stability of transferrin receptor (TfR) mRNA. We have taken advantage of the different mobilities of the human and rodent IRE/IRE-BP complexes on non-denaturing polyacrylamide gels to determine the chromosomal localization of the gene encoding the IRE-BP. Utilizing a panel of 34 different human/rodent hybrid cell lines we have assigned the IRE-BP gene to human chromosome 9. This new technique based on …

A Field Key To Fish Families Reported From South American Fresh Waters, C. Kelly Swing, John S. Ramsey

Occasional Papers of the Museum of Natural Science, Louisiana State University

No abstract provided.

Cloning And Sequencing Of The Eco Rii Monomer Fragment Of Satellite Dna From The Mouse Mus Musculus, Timothy Patrick Boyle

Masters Theses

Total mouse satellite DNA was isolated in cesium chloride gradients in the presence of the dye Hoechst 33258. This satellite fraction was digested with Bst Nl, an isoschizomer of the restriction enzyme Eco RII, and the 234 base pair monomer fragment was cloned into the plasmid vector pBS. Dideoxynucleotide sequencing of the cloned DNA yielded significant homology with the published consensus sequence of mouse satellite DNA (92%).

The Paradigm Of Huntington Disease, John B. Jenkins, P. M. Conneally

Biology Faculty Works

No abstract provided.

Southern Demersal Gillnet And Longline Fishery., Management Advisory Committee

Fisheries Management Papers

In its report to the Ministier for Fisheries in November 1986, the Southern Western Australian Shark Working Group recommended the establishment of an Industry/Government Mangement Advisory Committee to provide managememt advice on the fishery to the Minister for Fisheries. The Advisory Committee held its first meeting in Albany on 23 February 1989. After being briefed on the state of shark stocks, the Committee's deliberations fell into three broad categories. In the first category there was sufficient time and information available to make a recommendation to the Minister for Fisheries. In the second category, discussion commenced, but was held over to …

The T(11;14)(P15;Q11) In A T-Cell Acute Lymphoblastic Leukemia Cell Line Activates Multiple Transcripts, Including Ttg-1, A Gene Encoding A Potential Zinc Finger Protein, Elizabeth A. Mcguire, Richard D. Hockett, Karen M. Pollock, Marty F. Bartholdi, Stephen J. O'Brien, Stanley J. Korsmeyer

Biology Faculty Articles

Interchromosomal translocations within lymphoid neoplasms frequently involve the antigen receptor genes. We cloned the breakpoints of the t(11;14)(p15;q11) in a CD3-negative T-cell acute lymphoblastic leukemia cell line (RPMI 8402) in order to identify new genes potentially involved in T-cell neoplasia. An extensive comparison of both breakpoints and their germ line counterparts indicated that an inadvertant recombinase-mediated break at chromosome segment 11p15 recombined with the delta T-cell receptor at 14q11. The derivative 11 breakpoint resembles a coding joint in which 11p15 rather than a variable region was introduced 5' to a D_δ1D_δ2J_δ1 intermediate rearrangement. Conversely, the …

Variants And Polymorphisms Of Three Repetitive Dna Families In The Human Genome, Robert M. Roudabush

Electronic Theses and Dissertations

A novel 0.6 kb LINE family in human DNA, designated L2Hs, has been described (Musich and Dykes 1986). Studies employing clone N6.4, containing three 0.6 kb segments of this family, indicate that these sequences are interspersed and moderately repetitive. Two additional variant sequences of the L2Hs family, N6.1 and N6.3, have been identified. Restriction mapping of each cloned segment indicates similarities among N6.4, N6.3 and N6.1. When the cloned DNAs were cleaved with restriction enzymes and subjected to cross-hybridization, each cloned insert produced a pattern indicating that the sequences contained in N6.1 and N6.3 are represented in at least one …

Analysis Of Mitochondrial Dna Restriction Fragment Patterns In Killer Whales, Orcinus Orca, Tracy Alison Stevens

Dissertations and Theses

The mitochondrial DNA restriction fragment patterns of killer whales (Orcinus orca) were investigated in order to determine the level of genetic differentiation that exists between killer whales from various geographic locations. Twenty one killer whales were examined, seventeen of which were captive killer whales that originated from the North Atlantic and Northeast Pacific Oceans. Two were captive-born animals and two were killer whales that stranded along the Northeast Pacific coast.

DNA was extracted from blood and/or tissue samples, cleaved with a variety of restriction endonucleases and the DNA fragments were separated by horizontal agarose gel electrophoresis. The DNA …

Isolation And Partial Characterization Of Basic, Sperm-Specific, Nuclear Proteins In The Dog And Hampster, Richard William Hall

Biological Sciences Theses & Dissertations

The purpose was to determine whether dogs and hamsters contain the same sperm-specific protamine and testis specific proteins (TP) as other eutherian mammals previously studied. The proteins (protamine and TPs) were isolated from spermatids and mature spermatozoa, and were subjected to electrophoresis in an acid-urea, polyacrylamide gel system according to the procedure of Panyim and Chalkley (1962). The results indicated that, regardless of the anatomical location of sperm acquisition, acid precipitation technique, or denaturing agents used, epididymal and testicular samples from both the dog and hamster contained protamine in elongated spermatids and mature spermatozoa. The protamines from dogs and hamsters …

A Cytogenetic Examination Of Eight Species Of Tribolium (Coleoptera: Tenebrionidae), Lisa Anne Shimeld

Theses Digitization Project

No abstract provided.

Rock Lobster Industry Advisory Committee Report To The Minister For Fisheries., Rock Lobster Industry Advisory Committee

Fisheries Management Papers

The total catch of about 12.1 million kilograms for the 1987/88 season, based on processor returns, was an increase of 44% on the 1986/87 season. This very high catch, the third highest on record, resulted from an above average perulus settlement in 1983/84 and a "flow-on" of fast growing animals stemming from the expected high recruitment in 1988/990 caught as "reds" in 1987/88.

The Committee has identified five major elements related to the state of the rock lobster fishery upon which management advice is considered.

a) The undersize fraction of the population.

b) The "whites" which provide a significant proportion …

Breeding Field Peas In Western Australia, T N. Khan

Journal of the Department of Agriculture, Western Australia, Series 4

Field peas have grown dramatically in popularity in western Australia in the past five years, with the planted area increasing from a mere few hundred hectares to about 70,000 ha in 1988. This growth may continue, as more than 700,000 ha of agricultural land is potentially suitable for growing field peas.

However, any such growth of the pea industry will require new cultivars with improved yield, adaptation and quality characteristics to meet local and export demands. The Department of agriculture therefore decided to start a selection and breeding programme to support the pea industry.