Genetics and Genomics Commons^™

Breeding For Improved Feed Conversion Efficiency, Alan Lymbery

Journal of the Department of Agriculture, Western Australia, Series 4

Alan Lymbery reports on a trial that has started at Vasse Research Atation to investigate the impact of genetic improvement on feed conversion efficiency on costs of production.

Homozygosity Levels In Consanguineous Populations: A Study Of The Sankethi Community, Karnataka, India, Janet Cahill

Theses : Honours

The project has studied the inheritance of microsatellite alleles across four chromosomes within two multi-generation families from a highly endogamous community, the Sankethi of Karnataka, India. The two families each have members from four generations participating in the study. Finger prick blood samples and detailed pedigree information were collected from subjects in India. DNA was extracted from the blood spot samples using a phenol-chloroform technique, and analysed by spectrophotometry to determine DNA concentrations. Fluorescent markers from chromosomes 15, 16, 17 & 18 were amplified using polymerase chain reaction (PCR) techniques on a Perkin Elmer 96 well thermocycler. The PCR products …

Parasite Biodiversity And Emerging Pathogens: A Role For Systematics In Limiting Impacts On Genetic Resources, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Emergence of pathogenic organisms continues as a threat to overall biodiversity and genetic resources. Macroparasites including helminths constitute a potential threat to economically important resources in agriculture and conservation biology. Limitation of this threat can be achieved through survey and inventory for biodiversity and the application of systematics to understand the host range , biogeography and history of faunas. Systematics constitutes the foundation for recognition of endemic and introduced elements of faunas and the basis for predicting the behavior of pathogens introduced to new ecological settings or host groups. The basis for emergence of pathogens has both a deep historical …

Assignment Of Fragile Site 8e (Fra8e) To Human Chromosome Band 8q24.11 Adjacent To The Hereditary Multiple Exostoses 1 Gene And Two Overlapping Langer-Giedion Syndrome Deletion Endpoints, April L. Hill, Y. Harada, E. Takahashi, J. Hou, M. J. Wagner, D. E. Wells

Biology Faculty Publications

The distamycin A inducible fragile site, FRA8E, Fra(8) (q24.11) has been previously mapped to 8q24.11, proximal to the MYC gene, by fluorescent in situ hybridization (Takahasi et al., 1991). This fragile site has been shown to be present in about 1 out of every 140 healthy Japanese individuals (Takahasi et al., 1988). To more precisely map FRA8E, we have used cosmids isolated from the Langer-Giedion chromosomal region (LGCR) for FISH analysis. Langer-Giedion syndrome (LGS) is a contiguous gene syndrome characterized by chromosome deletions in Sq24.11 (Ludecke et al., 1991). One of the genes known to be involved in the etiology …

Circadian Clock-Controlled Genes Isolated From Neurospora Crassa Are Late Night- To Early Morning-Specific, Deborah Bell-Pedersen, Mari L. Shinohara, Jennifer J. Loros, Jay C. Dunlap

Dartmouth Scholarship

An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control of genes involved in clock output pathways. In the fungus Neurospora crassa, one pathway known to be under control of the clock is asexual spore (conidia) development. To understand more fully the processes that are regulated by the N. crassa circadian clock, systematic screens were carried out for genes that oscillate at the transcriptional level. Time-of-day-specific cDNA libraries were generated and used in differential screens to identify six new clock-controlled genes (ccgs). Transcripts specific for each of the ccgs …

Shark Bay World Heritage Property: Summary Of Public Submissions To The Draft Management Plan For Fish Resources, Kevin A. Francesconi

Fisheries Management Papers

In November 1994, the draft management plan for fish resources for the Shark Bay World Heritage Area (Fisheries Management Paper no 72) was released for public comment. This report summarises and analyses the 28 written public submissions received to the draft fisheries management plan.

Shark Bay Management Paper For Fish Resources, Fisheries Department Of Western Australia, Department Of Conservation And Land Management

Fisheries Management Papers

This paper deals with the management of fish resources in the Shark Bay World Heritage Property which are the responsibility of the Fisheries Department of Western Australia. It was prepared by the Fisheries Department in close liaison with the Department of Conservation and Land Management. The policies and guidelines proposed in this paper will be followed for a period not exceeding 10 years.

Pearling And Aquaculture In The Dampier Archipelago - Existing And Proposed Operations - A Report For Public Comment, Ben Fraser

Fisheries Management Papers

This discussion paper has been prepared by the Fisheries Department of WA as part of a consultative process to enable the public to comment on existing and proposed pearling and aquaculture activities in the Dampier Archipelago. This report follows on from Fisheries Management Report 90 - the Driscoll Report - and provides more detailed information about pearling and aquaculture to enable the public to consider and comment on individual projects.

Molecular Phylogeny And Typing Of Blackflies (Diptera: Simuliidae) That Serve As Vectors Of Human Or Bovine Onchocerciasis, Jianming Tang, Kenneth Pruess, Eddie W. Cupp, Thomas R. Unnasch

Department of Entomology: Faculty Publications

A subregion of the mitochondrial large subunit (16s) rRNA gene was amplified by polymerase chain reaction (PCR) from nine species of blackflies (Diptera: Simuliidae) which serve as natural or experimental vectors of human or bovine Onchocerca parasites. PCR products from each species of blackfly were tested by directed heteroduplex analysis (DHDA), and their genotypes established according to diagnostic banding patterns of the heteroduplex products. Three alleles of mitochondrial 16s rRNA were found to exist in members of the Simulium (Edwardsellum) damnosum sensu lato complex from West Africa, and two alleles were found in the Neotropical Simulium (Psilopelmia …

Offshore Constitutional Settlement 1995, H. G. Brayford, G. E. Lyon

Fisheries Management Papers

The Offshore Constitutional Settlement OCS is a legal arrangement between the Commonwealth and State Governments, and defines control over the fisheries which operate off each State of Australia. For the Western Australian coast, the first set of arrangements took effect in 1987. The OCS of 1995 specifies five arrangements, under which the fisheries inside the 200 nautical mile approximately 370 km limit of the Australian Fishing Zone AFZ come under either State, Commonwealth, or joint control.

A Report On The Issues Affecting The Use Of The Dampier Archipelago, Peter Driscoll

Fisheries Management Papers

This report was commissioned by the Minister for Fisheries following concerns about resource sharing, allocation of licences and leases for pearling and aquaculture, recreational and commercial fishing opportunities, and local residents continued ability to access the islands and marine areas of the Dampier Archipelago. The purpose of this report is to provide a scoping document for a marine planning study for the Dampier Archipelago for the Fisheries Department and the Minister for Fisheries.

Fishermen's Views On The Future Management Of The Rock Lobster Fishery: A Report Prepared On Behalf Of The Rock Lobster Industry Advisory Committee, The Marketing Centre, Rock Lobster Industry Advisory Committee

Fisheries Management Papers

In June 1995, a self-completion survey of fishermen in the rock lobster fishery was sent out by The Marketing Centre to 589 licensees. A total of 206 valid responses were received. The questionnaire was fairly long and dealt with a number of complex issues. As such, this response rate of 36.0% compares favorably with the average for postal surveys of around 40.0%. The following represents the main findings from the survey.

Same Fish - Different Rules - Proceedings Of The National Fisheries Management Network Workshop Held As Part Of The Third Australasian Fisheries Managers Conference August 1995, F. B. Prokop

Fisheries Management Papers

This workshop represents the third time that a specific recreational fisheries workshop has been held in conjunction with the Australasian Fisheries Managers Conference. It is designed to analyse the different management approaches from the various fisheries agencies which have responsibility for selected species. The case studies that make up the workshop have been selected to represent the diversity of situations including freshwater, marine, pelagic and invertebrate species.

Balancing The Scales: Access And Equity In Fisheries Management - Proceedings Of The Third Australasian Fisheries Managers Conference, Rottnest Island, Western Australia, 2-4 August 1995, Patricia Summerfield

Fisheries Management Papers

The papers reproduced in this volume were presented at the Third Australasian Fisheries Managers Conference held at Kingstown Environmental Education Centre, Rottnest Island, Western Australia, from 2-4 August 1995. Two presentations have not been received in full written form and one paper was not available for publication due to copyright being held elsewhere. Abstracts of these papers are included in the proceedings. The papers are arranged in order of presentation at the Conference. The scenarios used in the workshop sessions are reproduced at the end of the proceedings for information.

Management Arrangements For Specimen Shell Collection In Western Australia, 1995, Jonathon Hs Barrington, C A. Campbell

Fisheries Management Papers

The Specimen Shell Fishery in Western Australia is based on the collection of marine species of the phylum Mollusca for the purposes of display, collection, cataloguing, classification and sale. Specimen shells may be alive or dead at the time of take and include beach-washed shells. Fossilised shells do not constitute specimen shells. This document has been prepared to detail the legislative requirements of the Western Australian Government, as well as to fulfill the requirements under the Commonwealth Wildlife Protection (Regulation of Exports and Imports) Act 1982 to allow for the export of specimen shells from Western Australian waters and the …

Rosalind Elsie Franklin (1920-1958) Biologist, Margaret Sylvia

Faculty Publications

A biography of the biologist Rosalind Elsie Franklin whose work on x-ray crystallography contributed to Watson and Crick's elucidation of the structure of DNA.

A Gene Correlate Of Geotaxis Near Adh (2-50.1) In Drosophila Melanogaster, Scott F. Stoltenberg, Jerry Hirsch

Department of Psychology: Faculty Publications

As part of a long-term study (38+ years) to identify genetic correlates of geotaxis in Drosophila melanogaster, the investigators report the results of allozyme-level analyses of 2 lines derived from hybridizing high- and low-selected lines that have evolved stable, extreme expressions of geotaxis. Allelic variation at Adh was associated with geotactic performance in 1 hybrid-derived line, but not in another, after 66 free-mating generations beyond the F₂ generation. A second-chromosome gene correlate of geotaxis may lie within 1 map unit of Adh. Population genetic analyses suggest that there were different selection pressures on the hybrid-derived lines and …

Molecular Differentiation Of Alfalfa Weevil Strains (Coleoptera: Curculionidae), S. J. Erney, K. P. Pruess, S. D. Danielson, T. O. Powers

Department of Entomology: Faculty Publications

Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil, Hypera postica (Gyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes. No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska.

Microevolutionary Patterns And Molecular Markers: The Genetics Of Geographic Variation In Ascaris Suum, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Molecular markers have been used only rarely to characterize the population genetic structure of nematodes. Published studies have suggested that different taxa may show distinct genetic architectures. Isoenzyme and RAPD markers have been used to investigate geographic variation of Ascaris suum at the level of infrapopulations (nematodes within individual hosts), within localities, and among geographic regions. Independent estimates of genetic differentiation among population samples based on isoenzyme and RAPD data showed similar patterns and substantial correlation. Heterozygote deficiencies within infrapopulations and large values for inbreeding coefficients among infrapopulations suggested that the composition of these populations was not consistent with a …

Numerical Chromosome Abnormalities In Sperm From Oligoasthenoteratozoospermic Patients And Fertile Males, Myung-Geol Pang

Theses and Dissertations in Biomedical Sciences

Artificial fertilization protocols have been developed which bypass natural barriers for reproduction. One aspect of infertility which has received little attention is the potential importance of aneuploidy in sperm. To more clearly understand the cytogenetic make-up of sperm from OAT patients, multi-probe, multi-color FISH was performed to determine aneuploidy.

The introduction of intracytoplasmic sperm injection (ICSI) has revolutionized protocols used in in vitro fertilization centers. The pregnancies resulting from ICSI suggest an increased frequency of sex chromosome aneuploidy in livebirths. Preliminary data by others' suggest that sperm tail swelling patterns following hypo-osmotic swelling (HOST) can be predictive of fertilizing ability. …

A Review Of Ministerial Policy Guidelines For Rock Lobster Processing In Western Australia From The Working Group Appointed By The Minister For Fisheries And Chaired By Peter Rich, Peter Rich

Fisheries Management Papers

The rock lobster processing industry in Western Australia has a long history of management. Whilst management of the industry through the Fisheries Department has obviously been successful in helping to build the rock lobster industry into one of the State's major export industries, this approach is not without problems. With the introduction of the Fisheries Management Act 1994, the Minister for Fisheries deemed it appropriate to appoint an expert group to review the existing regulation of the rock lobster processing sector, draft the necessary guidelines to assist the Executive Director of the Fisheries Department make decisions on rock lobster processing …

Connexin32 Is A Myelin-Related Protein In The Pns And Cns, Steven S. Scherer, Suzanne M. Deschênes, Yi-Tian Xu, Judith B. Grinspan, Kenneth H. Fischbeck, David L. Paul

Biology Faculty Publications

We have examined the expression of a gap junction protein, connexin32 (Cx32), in Schwann cells and oligodendrocytes. In peripheral nerve, Cx32 is found in the paranodal myelin loops and Schmidt-Lanterman incisures of myelinating Schwann cells, and the levels of Cx32 protein and mRNA change in parallel with those of other myelin-related genes during development, Wallerian degeneration, and axonal regeneration. In the central nervous system, Cx32 is found in oligodendrocytes and their processes, but not in compact myelin, and the levels of Cx32 protein and mRNA increase during development in parallel with those of the other myelin genes. Thus, Cx32 is …

The Impact Of The New Management Package On Smaller Operators In The Western Rock Lobster Fishery, Ross Gould

Fisheries Management Papers

For the 1993/94 western rock lobster fishing season several new management strategies were introduced. The objective of the changes to the management package was to protect the rock lobster fishery. It was also to provide sufficient breeding stock to ensure viability of the industry. The most significant new measure was a temporary (two seasons) 18 percent pot reduction. Other changes were also made. There were considered sound reasons for reviewing and changing the previous management package. Despite this, some fishermen were critical of the new management package, and claimed that it unfairly disadvantaged smaller operators. Against this background, the Department …

West Coast Rock Lobster Fishery Management Plan 1995 - Draft For Public Comment, M. Moran

Fisheries Management Papers

The purpose of this management plan is to regulate fishing for rock lobsters (Family Palimuridae) in waters off the west coast of Western Australia such that the breeding stock of rock lobsters is maintained at a level known to have generated adequate recruitment of young lobsters in the past. This management plan does not constitute a change in the management arrangements for the rock lobster fishery. It essentially replaces the West Coast Rock Lobster Limited Entry Fishery Notice 1993 made under the Fisheries Act 1905 and makes the required changes to wording and structure necessitated by the proclamation of the …

Bag And Size Limit Fishing Regulations From Around Australia - Current Information As At 1 July, 1995 - Third Australasian Fisheries Managers Conference, Rottnest Island, Wa, F. B. Prokop

Fisheries Management Papers

This paper was originally prepared for the first National Fisheries Managers Conference in 1993. It is now updated and includes the current bag and size limits from around Australia as at 1 July, 1995. It is fairly obvious from this paper that there is a need for more cooperation between the states, particularly for species which have wide distributions. However, regional solutions can be a good approach for specific management needs, while general rules can be made more consistent in a number of cases.

Translocation Issues In Western Australia: Proceedings Of A Seminar And Workshop Held On 26th And 27th September 1994, F. B. Prokop

Fisheries Management Papers

The Recreational Fishing Advisory Committee (RFAC) approved holding this translocation seminar to air the translocation of fish for recreational enhancement in a public forum. This forum will identify issues including establishing common ground between the variety of interest groups which are represented. Contentious areas which require further investigation and consultation should become apparent, and assist the formulation of appropriate policies on translocation for Western Australia.

Investigation Of The Substrate Recognition Characteristics And Kinetics Of Mammalian Mitochondrial Dna Topoisomerase I, Zeki Topcu

Theses and Dissertations in Biomedical Sciences

Topoisomerases are DNA-modifying enzymes found in prokaryotes, eukaryotes, viruses and organelles such as chloroplast and mitochondria. Information about these enzymes in eukaryotic systems is mostly limited to nuclear enzymes, although our laboratory has been characterizing the biochemical and biophysical properties of the mammalian mitochondrial topoisomerases. We have determined the polarity of the attachment of mitochondrial topoisomerase I to its substrate DNA. To study the substrate preference and kinetic parameters of mitochondrial topoisomerase I, selected regions of mammalian mitochondrial DNA (mtDNA) were inserted into pGEM plasmid vectors following a series of modification and optimization experiments of currently available methods for PCR-cloning. …

Identification And Characterization Of Mitochondrial Dna Variants In Alzheimer's Disease, Natasha Singh Hamblet

Theses and Dissertations in Biomedical Sciences

Alzheimer's Disease (AD) is a complex neurodegenerative disorder that affects a significant portion of the human population regardless of ethnicity or gender. A mitochondrial hypothesis of AD has been proposed based on a number of studies which establish altered oxidative phosphorylation (OXPHOS) and ATP synthesis in AD tissue. ATP demand is most prevalent in the brain; damage to OXPHOS could severely impair brain metabolism, thereby leading to a decline in cognitive function. Four out of five complexes in the OXPHOS pathway are partly encoded by mitochondrial DNA (mtDNA); thus, this may be a crucial site of lesions that alter brain …

Management Options (Discussion Paper) For The Shark Bay Snapper Limited Entry Fishery, Shark Bay Snapper Limited Entry Fishery Working Group, Doug Bathgate

Fisheries Management Papers

At the Annual Management Meeting of the Shark Bay Snapper Limited Entry Fishery held on 22 July 1994 industry put forward a proposal that supplementary and non-transferable quota that was "lost" to the fishery when a licence holding non-transferable quota was transferred should be retained by the Fisheries Department and leased back to licence holders on an annual basis. As a result of this proposal and the fact that the management arrangements for the fishery are extremely complex and in need of simplification, the Minster for Fisheries approved the establishment of a Working Group to examine the lease-back proposal and …

Management Of The Northern Demersal Scalefish Fishery, Jane Fowler

Fisheries Management Papers

In mid 1994, the Fisheries Department of Western Australia announced that it would prepare a discussion paper setting out options for future management of the demersal scalefish fishery off the Kimberley coast. This paper, which was to be the start of a wider consultative process, was also to include options for possible further development of the trap and line fisheries. This paper is only the first step in a process for developing formal long term management arrangements under the new fisheries legislation, the Fish Resources Management Act 1994. Its aim is to provide some background and to present some management …