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Articles 211 - 240 of 5215

Full-Text Articles in Food Biotechnology

Effects Of Low-Pressure Electrostatic Field-Assisted Freezing At Different Field Strengths On The Quality Of Sea Buckthorn Leaves, Xiaoyue Dong, Tongliga Dong, Jinwei Te, Xiukuan Wang, Qian Hao, Meishu Miao, Xueyan Yun Sep 2025

Effects Of Low-Pressure Electrostatic Field-Assisted Freezing At Different Field Strengths On The Quality Of Sea Buckthorn Leaves, Xiaoyue Dong, Tongliga Dong, Jinwei Te, Xiukuan Wang, Qian Hao, Meishu Miao, Xueyan Yun

Journal of Food Science and Biotechnology

[Objective ] This study aimed to investigate the effects of different freezing preservation techniques on the quality of sea buckthorn (Hippophae rhamnoides ) leaves.[Method ] A low-voltage electrostatic field (LVEF ) pretreatment was applied during the freezing process of sea buckthorn leaves,and its effects on freezing characteristics and bioactive compound retention were evaluated.Field strengths at 0,20,40,and 60 cm from the discharge plate were compared with conventional freezing in terms of freezing rate,water loss rate,bioactive compound retention rate,and antioxidant activity of sea buckthorn leaves.The optimized and conventionally frozen leaves were subsequently used to prepare sea buckthorn leaf yogurt,with plain yogurt (NY) …

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Baking Degree Discrimination And Quality Prediction Of Tieguanyin Oolong Tea Based On Near-Infrared Spectroscopy, Lizhu Huang, Yun Bian, Zhenfeng Li, Xianfeng Li, Chunfang Song Sep 2025

Baking Degree Discrimination And Quality Prediction Of Tieguanyin Oolong Tea Based On Near-Infrared Spectroscopy, Lizhu Huang, Yun Bian, Zhenfeng Li, Xianfeng Li, Chunfang Song

Journal of Food Science and Biotechnology

[Objective ] This study aims to assess the quality of Tieguanyin oolong tea during the baking process.[Method ] Near-infrared spectroscopy was used for non-destructive detection of Tieguanyin oolong tea during the baking process and chemometrics was employed to analyze the changes in the intrinsic quality of the Tieguanyin oolong tea.Spectral preprocessing and dimensionality reduction were conducted for the near-infrared spectral data.Support vector machine (SVM ) and back propagation neural network (BPNN ) were adopted to construct discrimination models for the baking degree of Tieguanyin oolong tea.Combining the near-infrared spectral data with intrinsic quality data,partial least squares regression (PLSR ) was …

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Enhanced Expression Of Pepc Promotes The Synthesis Of 4-Hydroxyisoleucine By Corynebacterium Glutamicum, Chuhan Hu, Feng Shi Sep 2025

Enhanced Expression Of Pepc Promotes The Synthesis Of 4-Hydroxyisoleucine By Corynebacterium Glutamicum, Chuhan Hu, Feng Shi

Journal of Food Science and Biotechnology

[Objective ] This study aims to achieve the efficient biosynthesis of 4-hydroxyisoleucine (4-HIL ) in Corynebacterium glutamicum (C.glutamicum ).[Method ] The isoleucine (Ile) inducible promoters (PbrnFEN) of varying strengths were used to dynamically upregulate the expression of the isoleucine dioxygenase (IDO ) gene ido and the Vitreoscilla hemoglobin (VHb ) gene vgb.On this basis,constitutive promoters of different strengths were employed to enhance the expression of the phosphoenolpyruvate carboxylase (PEPC ) gene ppc and the pyruvate carboxylase gene pyc.[Result ] Among the recombinant strains harboring different P brnFEN promoters,strain C.glutamicum SN-I 7V7 (SN-I 7V7) was selected.After 144 h of fermentation with …

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Beneficial Effects Of Performlytetm, A Prodosomed Phytonutrient-Enriched Electrolyte Formulation, In Enhancing Neuro-Muscular Synergy, Immune Competence, Cellular Aerobic Glycolysis And Sports Performance, Bernard William Downs, Samudra P. Banik, Bruce S. Morrison, Tandra Chakraborty, Manashi Bagchi, Sanjoy Chakraborty, Steve Kushner, Debasis Bagchi Sep 2025

Beneficial Effects Of Performlytetm, A Prodosomed Phytonutrient-Enriched Electrolyte Formulation, In Enhancing Neuro-Muscular Synergy, Immune Competence, Cellular Aerobic Glycolysis And Sports Performance, Bernard William Downs, Samudra P. Banik, Bruce S. Morrison, Tandra Chakraborty, Manashi Bagchi, Sanjoy Chakraborty, Steve Kushner, Debasis Bagchi

Publications and Research

An optimised sports nutrition formulation achieving the correct blend of electrolytes with antioxidants and vitamins is integral towards meeting the demanding performance in professional sports arena. In this work, we describe a pilot study carried out to understand the effect of a novel phytonutrient- and electrolyte-fortified sports nutrition composition, PerformLyteTM, developed in our laboratories, in boosting the performance of trained male cyclists. PerformLyteTM is a unique fortified formulation comprising of polyphenolic antioxidants, flavonoids, and anthocyanins, as well as carotenoids, sulforaphanes, exotic spices, sea water-derived electrolytes, and vitamin C, encapsulated in proprietary patent-pending Prodosome absorption technology, enabling sustained …

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Advance S In The Generation And Transport Behavior Of Secondary Hazards From Food Packaging Sterilization, Mengjia Ren, Ye Liu, Jinpeng Wang, Shuang Bi Aug 2025

Advance S In The Generation And Transport Behavior Of Secondary Hazards From Food Packaging Sterilization, Mengjia Ren, Ye Liu, Jinpeng Wang, Shuang Bi

Journal of Food Science and Biotechnology

Food packaging sterilization is an important technical means to avoid the spread of SARS-CoV- 2 and the cross-contamination of foodborne viruses.However,the secondary hazards generated after sterilization are complex and have different characteristics,and a wide range of factors affect the formation of these hazards.The studies about the migration behavior of secondary hazards from packaging materials to food remain to be reviewed.Therefore,the author systematically reviewed the correspondence between sterilization technologies and secondary hazards generated after food packaging sterilization,the characteristics of secondary hazards,the factors affecting the formation of secondary hazards,and the detection technologies of secondary hazards.By outlining the classical migration model,the author summarized …

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Lipase-Mediated Oxidation Of Furfural To Furoic Acid, Rui Zhu, Yaxin Wang, Liang Zhang Aug 2025

Lipase-Mediated Oxidation Of Furfural To Furoic Acid, Rui Zhu, Yaxin Wang, Liang Zhang

Journal of Food Science and Biotechnology

[Objective ] This study aims to develop a lipase-mediated furfural oxidation system in ionic liquid for the green synthesis of furoic acid,5-hydroxymethyl- 2-furancarboxylic acid,and 2,5-furandicarboxylic acid under mild reaction conditions.[Method ] Using furfural as the substrate,Novozym 435 lipase as the catalyst,hydrogen peroxide as the oxidant,and 1-butyl- 3-methylimidazolium acetate ionic liquid as the solvent,the conditions including reaction temperature,reaction time,initial furfural concentration,and initial hydrogen peroxide concentration were optimized.The system was further extended to the oxidation of 5-hydroxymethylfurfural,and coupled with a chemical oxidation process using an imidazolium-based ionic liquid and tert-butyl hydroperoxide to achieve one-pot synthesis of 2,5-furandicarboxylic acid.[Result ] Under the optimized …

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Oral Administration Of Lacticaseibacillus Paracasei Ccfm 1433 Boosts Host Immunity To Prevent Candida Albicans Infections, Xinya Zhang, Qiuxiang Zhang, Xin Tang, Bingyong Mao, Jianxin Zhao, Shumao Cui Aug 2025

Oral Administration Of Lacticaseibacillus Paracasei Ccfm 1433 Boosts Host Immunity To Prevent Candida Albicans Infections, Xinya Zhang, Qiuxiang Zhang, Xin Tang, Bingyong Mao, Jianxin Zhao, Shumao Cui

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the role of Lactobacillus in enhancing host immunocompetence and preventing vaginal infections with Candida albicans.[Method ] A human vaginal epithelial cell (VK2/E6E7 cell) model was employed to screen the most potent strain among ten vaginal Lactobacillus isolates in inducing the antimicrobial peptide LL- 37.A mouse model of vaginal infection with Candida albicans was established.The effects of oral administration of the selected Lactobacillus strain on vaginal mucosal immunity,systemic inflammatory balance,and vaginal microbiota homeostasis were systematically evaluated using hematoxylin-eosin (HE) staining,ELISA,qPCR,and 16S rRNA sequencing.[Result ] Both t he cell lysate and fermentation supernatant of Lacticaseibacillus …

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Screening Of Excellent Lactic Acid Bacteria And Yeast Fermentation Agents And Effects Of Mixed Fermentation On The Quality Of Bread Dough, Yumeng Chen, Weijun Wang, Shuling Shen, Yanhua Li, Jingjing Ma, Menghan Huang, Yuhang Liu Aug 2025

Screening Of Excellent Lactic Acid Bacteria And Yeast Fermentation Agents And Effects Of Mixed Fermentation On The Quality Of Bread Dough, Yumeng Chen, Weijun Wang, Shuling Shen, Yanhua Li, Jingjing Ma, Menghan Huang, Yuhang Liu

Journal of Food Science and Biotechnology

[Objective ] The research on the synergistic use of lactic acid bacteria and yeast in sourdough is beneficial for meeting the industrial production demand of fermentation agents for baking food.[Method ] This study investigated the acid tolerance,acid production,enzyme production,gas production,and alcohol production abilities of various strains during the fermentation process by measuring the microbial count,pH,stretching area,elongation,hardness,viscosity,storage modulus (G′),loss modulus (G″),etc.of bread dough.The effects of different fer mentation agents on the total titratable acidity (TTA ),stretching characteristics,texture characteristics,and rheological properties of b read dough were investigated.[Result ] Lacticaseibacillus paracasei LG0260,Lactiplantibacillus plantarum LG1034,Lactococcus lactis LG0827,as well as Saccharomyces cerevisiae J2808 and S.cerevisiae …

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Structural Characteristics And Antibacterial Activity Of Silver Nanoparticles Prepared From Phyllanthus Emblica Extract, Jingyuan Liu, Wenting Wang Aug 2025

Structural Characteristics And Antibacterial Activity Of Silver Nanoparticles Prepared From Phyllanthus Emblica Extract, Jingyuan Liu, Wenting Wang

Journal of Food Science and Biotechnology

[Objective ] The aim of this study is to investigate the structural characteristics,antibacterial activity,and antioxidant capacity of silver nanoparticles (AgNPs ) prepared from Phyllanthus emblica extract.[Method ] AgNPs were prepared with Phyllanthus emblica extract as a reducing agent.The structure of the AgNPs was characterized by X-ray diffraction (XRD ),scanning electron microscopy (SEM ),photon correlation spectroscopy (PCS ),and transmission electron microscopy (TEM ).Furthermore,the inhibitory effects of AgNPs at different mass concentrations on Escherichia coli ATCC 25922,Staphylococcus aureus ATCC 25933,and Pseudomonas aeruginosa PAO 1 were assessed,with the minimum inhibitory concentrations (MIC ) being determined.In addition,the superoxide anion and ABTS+ free radical scavenging …

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Research Progress In Phytosterols (Esters ) And Their Application In Food, Shuang Mei, Huibing Luo, Tian Gan, Yonggang Qi, Chunmei Li, Chao Wang, Zao Liu Aug 2025

Research Progress In Phytosterols (Esters ) And Their Application In Food, Shuang Mei, Huibing Luo, Tian Gan, Yonggang Qi, Chunmei Li, Chao Wang, Zao Liu

Journal of Food Science and Biotechnology

Phytosterols esters are a type of compound synthesized through the esterification reaction between phytosterols and fatty acids.To clarify the research and application of phytosterols (esters ),the author provided an overview of their structural characteristics,synthesis methods,and physiological functions.Additionally,it was elucidated that phytosterols (esters ),as functional food ingredients,play a significant role in reducing cholesterol levels and exhibiting antioxidant properties in the human body.By delving into the bioactivity of phytosterols (esters ) and enhancing peopl e’s awareness of their nutritional value,this study makes an outlook on the research and application prospects of phytosterols (esters ) in food,thereby providing a crucial theoretical basis for …

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Efficient Catalytic Synthesis Of Allitol In Recombinant Escherichia Coli, Ying Cao, Peiyu Miao, Yun Liu, Zigang Zhang, Xian Zhang, Zhiming Rao Aug 2025

Efficient Catalytic Synthesis Of Allitol In Recombinant Escherichia Coli, Ying Cao, Peiyu Miao, Yun Liu, Zigang Zhang, Xian Zhang, Zhiming Rao

Journal of Food Science and Biotechnology

[Objective ] This study aims to enhanced the conversion efficiency of allitol through biocatalytic methods.[Methods ] The ribulose dehydrogenase (RDH ) genes rdh from Providencia alcalifaciens,Klebsiella pneumoniae and Klebsiella oxytoca were respectively expressed in Escherichia coli (E.coli ) BL21,designated as Pardh,Kprdh and Kordh respectively to identify the target genes with higher transformation rates.Further,the RDH from Klebsiella oxytoca was coupled with the formate dehydrogenase (FDH ) from Candida boidinii for co-catalysis,and the catalytic conditions and the addition amounts of co-substrates were optimized to achieve efficient enzymatic catalytic synthesis of allitol.[Results ] When using FDH and RDH from Klebsiella pneumoniae with an …

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Establishment Of A Hplc-Ms/Ms Method For Determination Of Cethromycin Residue In Poultry Eggs, Chenyang Chang, Liangzi Wang, Le Wang, Kai Chang, Jiao Shan, Xiao Wang, Liqiang Guo Aug 2025

Establishment Of A Hplc-Ms/Ms Method For Determination Of Cethromycin Residue In Poultry Eggs, Chenyang Chang, Liangzi Wang, Le Wang, Kai Chang, Jiao Shan, Xiao Wang, Liqiang Guo

Journal of Food Science and Biotechnology

[Objective ] This study aims to establish a method for determining the residual amount of cethromycin in poultry eggs based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS ).[Method ] Four representative poultry eggs (chicken eggs,duck eggs,goose eggs,and quail eggs ) were selected as the research matrices.Cethromycin was extracted with Mcllvaine-Na 2EDTA buffer solution,purified by hydrophilic-lipophilic balanced polymer solid-phase extraction column (HLB ),separated by a C 18 column with 0.1% (volume fraction ) formic acid water and acetonitrile as the mobile phase,and determined by HPLC-MS/MS in the multiple reaction monitoring (MRM ) mode combined with an external standard method.[Result ] …

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Isolation Of Three Strains Of Cordyceps Militaris And Determination Of Their Active Compounds And Antioxidant Effects, Yun Meng, Yifang Zhang, Jinduo Shi, Dun Wang Aug 2025

Isolation Of Three Strains Of Cordyceps Militaris And Determination Of Their Active Compounds And Antioxidant Effects, Yun Meng, Yifang Zhang, Jinduo Shi, Dun Wang

Journal of Food Science and Biotechnology

[Objective ] The strains with high-yield for active compounds such as cordycepin and cordycepic acid were screened from Cordyceps militaris (C.militaris ) collected in Shaanxi Hualongshan National Nature Reserve.[Method ] Three strains LH 3,LY1,and LY 9 of C.militaris were isolated and identified.The yields of the primary active compounds,hydroxyl radical scavenging activity,and cellular oxidative protection activity in the extracts of the mycelial and fermentation broth of each strain were determined.[Result ] The yields of cordycepic acid,crude polyphenols,and crude polysaccharides,which were the main active compounds of C.militaris,were the highest in the mycelia extract of strain LY 9,with values of 2.94,28.79,65.01 mg/g,respectively,while the …

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Bifidobacterium Adolescentis Fhnfq 5m4 Modulates Gut Microbiota And Alleviates Colitis In Mice, Yi Wang, Liming Huang, Xihua Yu, Liuruolan Li, Wenwei Lu, Hongchao Wang, Zhijian Zhang Aug 2025

Bifidobacterium Adolescentis Fhnfq 5m4 Modulates Gut Microbiota And Alleviates Colitis In Mice, Yi Wang, Liming Huang, Xihua Yu, Liuruolan Li, Wenwei Lu, Hongchao Wang, Zhijian Zhang

Journal of Food Science and Biotechnology

[Objective ] This study aims to verify and screen the Bifidobacterium adolescentis strains with vitamin A (VA) metabolic capacity,and investigate their effects on colitis symptoms and gut microbiota in mice.[Method ] The VA metabolic capacity of a panel of Bifidobacterium adolescentis strains was evaluated through in vitro fermentation,and the strain with the highest VA metabolic capacity was ultimately selected.This strain was administered orally to mice with dextran sulfate sodium (DSS )-induced colitis for 6 consecutive days.After the intervention,histopathological changes in the colonic tissue were assessed,and the levels of intestinal injury markers in the serum and cytokines,antioxidant enzymes,and retinoic acid (RA) …

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Screening And Characterization Of Yeast Strains From Naturally Fermented Rosa Roxburghii Pomace, Yong Li, Fang Yuan, Yu Fu, Luo Peng Aug 2025

Screening And Characterization Of Yeast Strains From Naturally Fermented Rosa Roxburghii Pomace, Yong Li, Fang Yuan, Yu Fu, Luo Peng

Journal of Food Science and Biotechnology

[Objective ] This study aimed to develop high-quality fermented Rosa roxburghii (R.roxburghii ) products by screening yeast strains suitable for R.roxburghii fermentation.[Method ] The naturally fermented R.roxburghi pomace was used as the material for preliminary screening through the TTC colorimetric method and secondary screening based on fermentation capacity.The tolerance of strains to SO 2,ethanol,low pH,and glucose was examined.The strains were identified via morphological characterization and molecular biological techniques.[Result ] Three high-performing strains (B17,B19,and B29) were obtained.These strains exhibited tolerance to 250 mg/L SO 2,mass fraction 60% glucose,volume fraction 8% ethanol,and pH 3.5 environment.B17 and B 29 were identified as Pichia …

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Isolation , Identification , And Whole Genome Sequencing Of Lactic Acid Bacteria From Pit Mud Of Jiangxiangxing Baijiu, Heou Huang, Cheng Wu, Shaojuan Yang, Dan Xie, Wenyu Zhao, Junlin Yang, Jianfeng Hu, Feng Hu, Diqiang Wang Aug 2025

Isolation , Identification , And Whole Genome Sequencing Of Lactic Acid Bacteria From Pit Mud Of Jiangxiangxing Baijiu, Heou Huang, Cheng Wu, Shaojuan Yang, Dan Xie, Wenyu Zhao, Junlin Yang, Jianfeng Hu, Feng Hu, Diqiang Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to develop functional microbial strain resources from the pit mud of Jiangxiangxing Baijiu and explore their potential in optimizing the fermentation process and improving Baijiu quality.[Method ] Lactic acid bacteria were screened from the pit mud of Jiangxiangxing Baijiu by the spread plate method and then identified by morphological and molecular biological methods.The metabolites of the strains were detected by high performance liquid chromatography and gas chromatography,and the whole genomes of the strains were sequenced via the Illumina sequencing technology.[Result ] Eleven strains of lactic acid bacteria were isolated and screened from the pit mud …

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Extraction Process Optimization And Antioxidant And Blood Glucose-Lowering Effect Evaluation Of Polyphenols From Camellia Japonica Flowers, Long Chen, Xiaocong Yang, Shujie Xing, Xinyao Shi, Xueke Zheng, Jing Zhu Aug 2025

Extraction Process Optimization And Antioxidant And Blood Glucose-Lowering Effect Evaluation Of Polyphenols From Camellia Japonica Flowers, Long Chen, Xiaocong Yang, Shujie Xing, Xinyao Shi, Xueke Zheng, Jing Zhu

Journal of Food Science and Biotechnology

[Objective ] This study optimized the extraction process of polyphenols from Camellia japonica (C.japonica ) flowers and preliminarily explored the antioxidant and blood glucose-lowering effects of the extracted polyphenols,aiming to improve the utilization of C.japonica flowers.[Method ] With the flowers of Xinyang Maojian as raw materials,the ultrasonic-assisted method was adopted to extract polyphenols from C.japonica flowers,and the antioxidant and blood glucose-lowering activities of the extracted polyphenols were analyzed.With the extraction rate of polyphenols from C.japonica flowers as the evaluation index,the response surface methodology was adopted to explore the effects of ethanol volume fraction,solid-liquid ratio,extraction time,and ultrasonic power on the extraction …

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Site-Directed Mutagenesis And Covalent Organic Framework Immobilization Of Serine Carboxypeptidase Y From Aspergillus Oryzae M30011 For Synergistic Enhancement Of Enzyme Performance, Jiawen Jiang, Bin Cai, Ke Xiong, Shucan Mao, Yiqiang Chen, Songlin Li, Pengcheng Zhang, Wei Yang, Borui Li Aug 2025

Site-Directed Mutagenesis And Covalent Organic Framework Immobilization Of Serine Carboxypeptidase Y From Aspergillus Oryzae M30011 For Synergistic Enhancement Of Enzyme Performance, Jiawen Jiang, Bin Cai, Ke Xiong, Shucan Mao, Yiqiang Chen, Songlin Li, Pengcheng Zhang, Wei Yang, Borui Li

Journal of Food Science and Biotechnology

[Objective ] To enhance the stability of serine carboxypeptidase during enzymatic hydrolysis and expand its industrial applicability,the author employed rational design and immobilization technology to develop efficient and stable enzymatic catalysts in view of the easy deactivation of this enzyme.[Method ] On the basis of the serine carboxypeptidase Y (CPY ) derived from Aspergillus oryzae M30011,single mutant enzymes M 517R,I464R,and Y 271R and the triple mutant enzyme M 517R/I464R/Y271R were designed.These mutant enzymes were then prokaryotically expressed in Escherichia coli and subjected to enzyme activity assays.Subsequently,the mutant enzyme with the highest activity was immobilized with covalent organic framework materials (COFs …

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Inhibition Mechanisms Of Naringenin And Egcg Against The Formation Of The Heterocyclic Amine Phip, Shuai Yu, Qingchen Luo, Mengmeng Feng, Xin Ai, Yubin Li, Liang Zhao Aug 2025

Inhibition Mechanisms Of Naringenin And Egcg Against The Formation Of The Heterocyclic Amine Phip, Shuai Yu, Qingchen Luo, Mengmeng Feng, Xin Ai, Yubin Li, Liang Zhao

Journal of Food Science and Biotechnology

[Objective ] This study clarifies the key roles of naringenin and epigallocatechin gallate (EGCG ) in inhibiting 2-amino- 1-methyl- 6-phenylimidazo [4,5-b]pyridine (PhlP ) formation in roasted meat and reveals the molecular mechanisms by which naringenin and EGCG regulate precursor consumption and intermediate accumulation,aiming to provide a scientific basis for reducing the risk of heterocyclic amines in food processing.[Method ] The changes in precursors of PhIP in roasted chicken breast were determined,and a model reaction system of flavonoids,methylglyoxal (MGO ),and phenylalanine (Phe) was established.The reaction kinetics curves of key substances were studied to decipher the inhibition mechanisms of naringenin and EGCG …

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Co-Expression And Application Of Enzymes For Preparation Of Β-Nicotinamide Mononucleotide, Jiehu Liu, Lingqia Su, Huixian Fu, Jing Wu Aug 2025

Co-Expression And Application Of Enzymes For Preparation Of Β-Nicotinamide Mononucleotide, Jiehu Liu, Lingqia Su, Huixian Fu, Jing Wu

Journal of Food Science and Biotechnology

[Objective ] This study aims to reduce the cost and simplify the production process of β-nicotinamide mononucleotide (β-NMN ) by the enzyme catalytic method.[Method ] On the basis of the previously established β-NMN synthesis system with six enzymes,the six enzyme genes were combined in pairs to construct double-enzyme co-expression recombinant strains.Each crude enzyme solution obtained by co-expression was used for multi-enzyme catalytic cascade reaction to synthesize β-NMN.[Result ] The yield of β-NMN was as high as 72.90% when the concentration of the substrate D-ribose was 10 mmol/L.The optimal temperature and IPTG induction concentration for inducing the enzyme expression of the …

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Effects Of Exogenous Enzyme Treatment On Quality And Main Components Of Summer Green Tea, Shanmin Chen, Hongyu Luo, Yi Wang, Rui Chang, Linying Yuan, Juan Yang Aug 2025

Effects Of Exogenous Enzyme Treatment On Quality And Main Components Of Summer Green Tea, Shanmin Chen, Hongyu Luo, Yi Wang, Rui Chang, Linying Yuan, Juan Yang

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effect of exogenous enzyme treatment on the quality of summer green tea and optimize the enzyme treatment conditions.[Method ] Sensory evaluation was employed to evaluate and screen the tea samples treated with six exogenous enzymes.The effects of cellulase,tannase,and treatment conditions on the sensory quality,bitter/astringent taste attributes,and main biochemical components of summer green tea were studied by electronic tongue and chemometrics methods.The optimal treatment conditions of cellulase and tannase were comprehensively evaluated by hierarchical cluster analysis,correlation analysis,and principal component analysis (PCA ).[Result ] Sensory evaluation revealed that cellulase and tannase significantly improved the …

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Preparation Of Human Asprosin Nanobodies Based On A Natural Nanobody Phage Display Library, Yun Liang, Yi Huang, Qinghua He Jul 2025

Preparation Of Human Asprosin Nanobodies Based On A Natural Nanobody Phage Display Library, Yun Liang, Yi Huang, Qinghua He

Journal of Food Science and Biotechnology

[Objective ] Asprosin (ASP ),an adipokine produced from fibrillin- 1 (FBN 1) cleavage,is closely linked to type 2 diabetes mellitus and obesity when its level is abnormal,demonstrating significant effect on human health.Therefore,monitoring the ASP level is crucial.The author aims to screen the nanobodies specifically binding to ASP via phage display technology,offering a new antibody component for the research on ASP.[Method ] The antigenic epitopes of ASP were predicted by the online tools ABCpred and NovoPro,and then coupled with bovine serum albumin (BSA ) to form the coating antigen (ASP-Epitope-BSA ).After five rounds of bio-panning,the nanobodies specifically binding to ASP were …

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Modulatory Effects Of Flavonoids On Il- 4/Il-13 In Atopic Dermatitis And Their Structure-Activity Relationships, Yueyuan Wang, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao Jul 2025

Modulatory Effects Of Flavonoids On Il- 4/Il-13 In Atopic Dermatitis And Their Structure-Activity Relationships, Yueyuan Wang, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the ameliorating effects of flavonoids on atopic dermatitis (AD) and their structure-activity relationships.[Method ] Flavonoids were selected through network pharmacology with interleukin- 4 (IL-4) and interleukin- 13 (IL-13) as key targets.The immunoglobulin E (IgE)-induced RBL- 2H3 cell model was employed to evaluate the effects of flavonoids on inflammatory factors,degranulation activities,and calcium ion influx,and the structure-activity relationships were analyzed.Finally,molecular docking was utilized to assist in exploring the action mechanism.[Result ] All of the six flavonoids (luteolin,apigenin,chrysin,quercetin,kaempferol,and fisetin ) screened by network pharmacology had significant inhibitory effects on the expression of IL- 4,IL- 13,tumor necrosis …

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Structural And Solution Property Characterization Of Oat Β-Glucans, Hongli Zhang, Yaning Shi, Ming Miao Jul 2025

Structural And Solution Property Characterization Of Oat Β-Glucans, Hongli Zhang, Yaning Shi, Ming Miao

Journal of Food Science and Biotechnology

[Objective ] This study aims to characterize the structure and investigate the solution properties of oat β-glucan (OBG ).[Method ] Two soluble OBGs with different weigh-average molecular weights (Mw),number-average molar weights (Mn),and peak molecular weights (Mp) were prepared by hot water extraction and ethanol classification.The structures and solution properties were characterized by Fourier-transform infrared spectroscopy (FT-IR ),nuclear magnetic resonance (NMR ),X-ray diffraction (XRD ),scanning electron microscopy (SEM ),high performance size exclusion chromatography coupled with multi-angle laser light scattering (HPSEC-MALLS ),and rheology.[Result ] OBG- 1 and OBG- 2 showed the Mw of 80.73×104 g/mol and 8.05×104 g/mol,the yields of (4.86±0.55)% and …

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Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots, Junjie Sun, Xiaoxia Liu, Ziyi Chen, Yinlai Li, Lu Zhai, Qinzhou Chen, Renyao Jin Jul 2025

Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots, Junjie Sun, Xiaoxia Liu, Ziyi Chen, Yinlai Li, Lu Zhai, Qinzhou Chen, Renyao Jin

Journal of Food Science and Biotechnology

[Objective ] This study aims to establish a rapid immunoassay method for the quantitative detection of eugenol.[Method ] The eugenol monoclonal antibody was conjugated to the surface of quantum dots by the 1-ethyl- 3- (3-dimethylaminopropyl ) carbodiimide (EDC ) and N-hydroxy-succinamide (NHS ) (EDC-NHS ) coupling method,with quantum dots as fluorescent markers.Bovine serum albumin (BSA ) was used for blocking,and fluorescence microscopy,agarose gel electrophoresis,and a microplate reader were used for identification.Furthermore,the established method was compared with high performance liquid chromatography (HPLC ).[Result ] The optimal synthesis conditions were as follows:quantum dot activation time of 30 min,conjugation pH with 8.0,antibody addition …

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Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch, Lei Lei, Wenjun Fu, Lingling Yang, Haibo Liu, Daxi Sang, Qing Wang Jul 2025

Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch, Lei Lei, Wenjun Fu, Lingling Yang, Haibo Liu, Daxi Sang, Qing Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the properties of chestnut starch.[Method ] Tea polysaccharides were added to chestnut starch in different proportions,and the effects of tea polysaccharides on the water-holding capacity,digestive properties,textural properties,and other properties of chestnut starch were investigated.[Result ] The water-holding and oil-holding properties of chestnut starch were the largest when tea polysaccharides were added at 1% and then gradually decreased with the increase in the proportion of tea polysaccharides added.The transparency of chestnut starch gradually increased when tea polysaccharides were added from 1% to 8% and decreased significantly when tea polysaccharides were added at 16%.Adding tea …

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Research Progress In Thermo-Reversible Polysaccharide Gels, Dongxin Zhou, Ziao Zhang, Su Chen, Weimin Yang, Jiajun Sun, Shengzu Han, Anning Xie, Xiaoxiao Li, Yuxiang Bai Jul 2025

Research Progress In Thermo-Reversible Polysaccharide Gels, Dongxin Zhou, Ziao Zhang, Su Chen, Weimin Yang, Jiajun Sun, Shengzu Han, Anning Xie, Xiaoxiao Li, Yuxiang Bai

Journal of Food Science and Biotechnology

Thermo-reversible gels are widely applied in the processing of baking,meat,and snack products including cakes,candies,jelly,aspic,and steamed custard buns.Currently,the most effective thermo-reversible gel is gelatin,which can combine with water to form a transparent gel that melts when it is close to the temperature of the human oral cavity.However,gelatin is expensive.Therefore,researchers have turned their attention to polysaccharide gels,hoping to obtain more thermo-reversible food raw materials with different properties.The author reviews thermo-reversible polysaccharide gels from natural sources such as plants,algae,and microorganisms and those modified by physical,chemical,and enzymatic methods and summarizes the sources,structures,gelatinization mechanisms,and product applications of polysaccharide gels.It is expected to provide a reference …

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Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases, Yixiao Shao, Chuang Huang, Chenjian Liu, Xiaoran Li Jul 2025

Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases, Yixiao Shao, Chuang Huang, Chenjian Liu, Xiaoran Li

Journal of Food Science and Biotechnology

The antioxidant properties of lactic acid bacteria are related to various probiotic activities,which can prevent and alleviate a variety of chronic diseases caused by oxidation in the host.The antioxidant mechanisms of lactic acid bacteria,including the action mechanisms of antioxidant enzymes and non-enzymatic antioxidants and regulation of host signaling pathways,were expounded.Furthermore,the application status of antioxidant properties of lactic acid bacteria in diseases of the nervous system,digestive system,and skin system were summarized.The aim is to explore how to enhance the antioxidant activity of lactic acid bacteria in oxidative stress-related diseases and give new insights into the development of related treatment strategies.

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Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38, Cong Gao, Weijia Yuan, Chao Ye, Jia Liu, Liming Liu Jul 2025

Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38, Cong Gao, Weijia Yuan, Chao Ye, Jia Liu, Liming Liu

Journal of Food Science and Biotechnology

[Objective ] A genome-scale metabolic model (GSMM ) of the high L-malic acid-producing strain Aspergillus oryzae (A.oryzae ) FMME-S- 38 was constructed and analyzed.[Method ] The new GSMM iJW1333 was established for A.oryzae FMME-S- 38 through a two-step process of draft reconstruction and model refinement.[Result ] The model integrated 1 723 biochemical reactions,1 723 metabolites,and 1 333 functional genes,achieving the prediction accuracy of 94.6% for substrate metabolism and 92.0% for cell growth.Comparative genomic analysis revealed that mutations in the genes involved in the hexose monophosphate pathway (HMP ) and oxidative phosphorylation underpinned the enhanced L-malic acid production of A.oryzae FMME-S- …

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Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins, Haibo Zhang, Yongjun Xia, Guangqiang Wang, Ning Peng, Zhixian Chen, Lianzhong Ai Jul 2025

Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins, Haibo Zhang, Yongjun Xia, Guangqiang Wang, Ning Peng, Zhixian Chen, Lianzhong Ai

Journal of Food Science and Biotechnology

[Objective ] The extraction efficiency of different methods and the effects of these methods on the composition of mannoproteins extracted from yeast cell walls were compared.Furthermore,the anti-inflammatory activities of the extracted mannoprotein were evaluated.[Method ] Three yeast mannoproteins (MP-W,MP-B,and MP-E ) were extracted via high-temperature water,alkali,and enzyme-assisted extraction methods and purified via ethanol precipitation.The identification of monosaccharide composition and amino acid composition,distribution of relative molecular mass,and Fourier transform infrared spectroscopy were employed to analyze the composition and chemical structures of yeast mannoproteins.A cell experiment was carried out to examine the anti-inflammatory activity.[Result ] The yields of yeast mannoproteins extracted by …

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