Food Biotechnology Commons^™

Effect Of Frozen Storage Time On The Oxidation Degree Of Porcine Myofibrillar Protein, Li Qian, Yu Li-Gang, Yang Yu-Kun, Li Mei-Ping, Guo Cai-Xia

Food and Machinery

In this study, myofibrillar protein was used as the research object to investigate the effect of different frozen storage time on the oxidation degree of myofibrillar protein of pork. Raw pork was stored at 18 ℃ for different time (0, 1, 4, 8, 12 weeks), and used for the extraction of myofibrillar protein. Then protein oxidation parameters (carbonyl, total sulfhydryl group, free amino groups, surface hydrophobicity and endogenous tryptophan fluorescence) were determined. The results suggested that, carbonyl content increased significantly (P＜0.05), and total sulfhydryl group, free amino groups, surface hydrophobicity and endogenous tryptophan fluorescence content generally showed a decreasing trend …

Research On Defect Detection And Classification Of Jujube Based On Improved Residual Network, Wen Huai-Xing, Wang Jun-Jie, Han Fang

Food and Machinery

In this paper, an algorithm based on deep residual network is proposed to recognize and classify the surface defects and texture of jujube. The algorithm adopted jujube’s G channel of RGB color figure then to get the characteristics of the figure as network input, using residual learning way to expand the depth of the neural network learning, and residual error of the neural network activation function Relu replaced with SELU, the loss function softmax loss with center loss to replace, Dropout layer was developed for the training, reduce the risk of network through fitting, solved with deepening study depth gradient …

Study On Purification And Antibacterial Activity Of Protamine From Takifugu Obscurus, Zhang Jia-Yuan, Chen Shun-Sheng, Zhang Hong-Cai

Food and Machinery

In this study, Sephadex G-50 and CM Sepharose Fast Flow were used to purify the spermatozoa of Takifugu obscurus. Identification of protamine by arginine color reaction. The effects of protamine on the minimum inhibitory concentration, inhibition zone diameter and growth curve of Staphylococcus aureus, Escherichia coli and Salmonella were determined. The results showed that: The spermatozoa of Takifugu obscurus had an absorption peak at 217 and 273 nm, respectively, and the absorption peak at 217 nm was higher. The purified protamine was found to be better than the protamine before purification by SDS-PAGE electrophoresis. The strips were fine and …

Effects Of Active Peptide Components Of Eupolyphaga Steleophaga On Blood Rheology, Four Indicators Of Blood Lipids And Blood Factor Levels In Rats With Acute Blood Stasis, Jiang Shan, Wang Shao-Ping, Dai Long, Zhang Jia-Yu, Gao Peng, Liu Zi-Han, Wang Yu-Qi

Food and Machinery

Pepsin and trypsin complex enzymes were used for biomimetic enzymatic hydrolysis of Eupolyphaga steleophaga. In order to obtain the most active fibrinolytic activity of the soil aphid active peptide component F2, the product was subjected to desalting, ultrafiltration, nanofiltration, DA201-C macroporous adsorption resin, dextran gel G-25 chromatography, reverse high performance liquid chromatography and semi-preparative liquid chromatography. Moreover, its effects on hemorheology index, blood lipids and serum nitric oxide (NO), superoxide dismutase (SOD), tissue plasminogen activator (t-PA) and fibrin (FIB) content of rats with acute blood stasis were also studied. E. ste-leophaga active peptide component F2 could significantly reduce blood …

Synergistic Effects Of Dendrobium And Pueraria Thomsonii On Hpyerglycemic And Lipid-Lowing Between In Vitro And In Vivo, Xu Zi-Yang, Jing Si-Qun, Lin Yin-Jun, Ding Jia-Min, Zhou Yang, Yu Bai-Yin

Food and Machinery

The synergistic effects were observed on inhibiting alpha-glucosidase activity between Dendrobium and Pueraria thomsonii form self-made chewable tablets by taking the acarbose as control. Drosophila melanogaster was used as experimental animal, and the model of Ⅱ diabetic Drosophila melanogaster was prepared by high glucose and high fat feeding. The experiment was divided into model control group, positive control group, Dendrobium (D) group, Pueraria thomsonii (PT) group and (Dendrobium + Pueraria thomsonii) (D+PT) group. After 7 days of lipid-lowering effects between Dendrobium and Pueraria thomsonii was analyzed. results showed that all of groups dose-effect perfusion, the total protein, triglycerides …

Optimization Of The Extraction Process Of Polysaccharides From Euglena And Its Antioxidant Activity, Ge Zhi-Chao, Li Yan, Shi Wen-Zheng, Lang Meng

Food and Machinery

Taking the euglena as the raw material, the extraction of euglena polysaccharide by ultrasonic-assisted enzymatic method and the optimal extraction conditions were studied, from the ultrasonic power, ultrasonic time, enzymolysis time, and enzyme concentration by response surface analysis(RSA) based on single factor tests. The results showed that the optimal process parameters were: ultrasonic power 360 W, ultrasonic time 19 min, neutral protease addition 3.7%, enzymatic hydrolysis time 3.5 h. Under the conditions, the yield of euglena polysaccharide reached 3.68%. The euglena polysaccharide (EGPS) was separated to three different ranges of molecular weight by ultrafiltration membrane, which were 35, 510 and …

Effect Of Haematococcus Pluvialis On Baking Quality Of Bread, Wang Bao-Bei, Dai Zi-Wei, Liu Lu-Lu, Chen Wen-Xuan

Food and Machinery

In this study, H. pluvialis was applied into bread to study its effect on baking quality. The results showed that the addition of H. pluvialis increased redness and yellowness of bread, and decreased the brightness. The specific volume of bread decreased slightly, but the moisture content did not change a lot with the addition of H. pluvialis. Within the content range studied, the hardness, stickiness and chewiness of bread increased gradually with increasing H. pluvialis, while the elasticity, resilience and cohesion did not change significantly. Similarly, during storage process, H. pluvialis had little effect on the changes of …

Effect Of Wheat Flour Substitution Withlily Powder On Properties Of Dough And Quality Of Bread, Li Jia-Yi, Tan Xuan-Yu, Zou Jin-Hao, Wang Feng, Su Xiao-Jun

Food and Machinery

In order to broaden the application of lily flour, wheat powder substituted with lily flour was applied in bread to improve nutrient value. The effects of wheat flour substitution with lily powder (0%, 5%, 10%, 15%, and 20%) on the microstruc-ture, pasting properties and rheological properties of dough and the quality of bread were investigated. The results showed that the water holding capacity increased significantly (P＜0.05) as the percentage of lily powder increased, while oil holding capacity first decreased and then increased. The addition of lily flour destroyed the compact and uniform microstructure of gluten. The pasting temperature of the …

Effects Of Different Pretreatment Methods On The Quality Characteristics Of Red Rose By Hot Air Drying, Zhang Meng, Duan Xu, Ren Guang-Yue, Zhou Si-Qing, Xu Yi-Ming

Food and Machinery

In order to understand the effect of different pretreatments on the hot air drying characteristics and quality of red rose, four different pretreatment methods ,i.e. impregnated 5% citric acid solution, 20% sugar solution, 20% NaCl and citric acid mixed solution and blanching, the pretreated petals were used as the control, to conduct comparative experiments and analysis of hot air drying. Sensory properties (color, total flavonoids, total phenols) and microstructure of red rose were examined, and combined with fuzzy mathematical comprehensive evaluation method for sensory evaluation. The results showed that drying rate of rose petals was influenced significantly by different pretreatment …

Study On Synchronous Extraction Process Of Oil And Protein From Prunus Mira Nut And The Oil Quality Analysis, Kan Jin-Tao, Yuan Lei, Zhong Zheng-Chang

Food and Machinery

Taking the Prunus mira nut as raw material, the oil and protein of were synchronous extracted by aqueous extraction method combined with enzymatic demulsification, the extraction process was optimized by orthogonal tests based on single factor experiment. The main physicochemical indexes of Prunus mira nut oil were determined, and the fatty acid compositions were analyzed by GC. The results indicated that the Prunus mira nut protein yields were(78.13±1.53)%, and oil total yields were(67.79±2.97)%, the optimal oil and protein extraction process were obtained as followed: sample size 150 mesh, liquid-solid ratio 8：1 (mL/g), pH 10, extraction temperature 50 ℃, extraction time …

Optimization Of Fermentation Process And Analysis Of Antioxidant Capacity Of Purple Sweet Potato Powder, Song Ying, Liu Si-Han, Chang Xia, Hou Yu-Hao, Xie Qiu-Tao, Yuan Hong-Yan, Li Gao-Yang

Food and Machinery

Based on the single factors, the response surface tests were used to optimize the process conditions of low-sugar yeast fermentation of the purple sweet potato to prepare high antioxidant activity powder, and the changes of total flavonoids, total phenols, anthocyanins and antioxidant capacity before and after fermentation were investigated. The optimum fermented conditions were determined as follows: 0.95% yeast addition, 10：22 (g/mL) ratio of feed to liquid, fermentation at 23 ℃ for 16 h. The antioxidant capacity was 404.94 mg VCEAC/100 g, with a total phenol content of 123.85 mg GAE/100 g, a total flavonoid content of 610.64 mg rutin/100 …

Research Progress On Anaerobic Fermentation Mechanism And High-Density Anaerobic Fermentation Of Clostridium Butyrate, Wu Tian-Fu, Xie Xiao-Lin, Chen Mei-Biao, Zhou Lian, Chen Meng, Deng Ming-Rong, Yao Qing, Zhu Hong-Hui

Food and Machinery

In this paper, the mechanism of anaerobic fermentation of Clostridium butyrate, the mode of high-density anaerobic fermentation and the application of bioreactor are rviewed in order to realize the high-density fermentation of Clostridium butyrate in industrial production by improving the anaerobic fermentation process or improving the fermentation equipment, so as to greatly increase the number of live bacteria and reduce the production cost.

Research Progress On Degradation Technology Of Harmful Gases In Granary, Pan Di, Ma Ting-Jun, Kang Kai, Bai Shu-Pei

Food and Machinery

There were many harmful gases in the process of storing grain in large granaries, which can not only cause environmental pollutions, but also endanger people's health. Reducing harmful gases in granaries and ensuring grain quality had become the focus of food storage industry. In this paper, the sources, types and harms of harmful gases produced in the process of grain storage were briefly described, and the relative degradation methods, principles, advantages and disadvantages of phosphine, were also introduced in detail. Furthermore, the feasibility of degradation of harmful gases in granary by plasma technology was emphasized, and the problems to be …

Labeling Compliance And Species Authentication Of Fish Fillets Sold At Grocery Stores In Southern California, Priscila Liou, Angela Banda, Rachel B. Isaacs, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Seafood mislabeling has numerous consequences, including economic deception and food safety risks. The focus of this study was to investigate fish species labeling, use of acceptable market names, and Country of Origin Labeling (COOL) compliance for fresh fish fillets sold at grocery store seafood counters in Southern California. A total of 120 fillets representing 16 different categories of fish were collected from 30 Perishable Agricultural Commodities Act (PACA)-listed grocery stores. Each sample underwent DNA barcoding to identify the species. Acceptable market names were confirmed using the FDA Seafood List. Samples were determined to be compliant with COOL if both …

Sodium Propionate And Sodium Butyrate Promote Fatty Acid Oxidation In Hepg2 Cells Under Oxidative Stress, Kristina Jazmin Cook

LSU Doctoral Dissertations

Non-alcoholic fatty liver disease (NAFLD) is a spectrum of disturbances that includes simple steatosis, non-alcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and liver cancer. NAFLD affects individuals that consume the typical Western diet consisting of high levels of fats and carbohydrates. The increase in circulating free fatty acids, palmitate and oleate, or lipopolysaccharides (LPS), induce oxidative stress and pro-inflammatory cytokine production in the liver, which all contribute to NAFLD progression. In this study, we are evaluating the mRNA expression of genes associated with fatty acid oxidation (FAO) and the protein expression of pro-inflammatory cytokines related to NAFLD using the HepG2 human liver …

An Evaluation Of Sonication Pretreatment For Enhancing Saccharification Of Brewers’ Spent Grain, Shady Hassan, Rajeev Ravindran, Swarna Jaiswal, Brijesh K. Tiwari, Gwilym A. Williams

Articles

This paper deals with the investigation of ultrasound (US) pretreatment of brewer’s spent grain (BSG) as ameans of releasing fermentable sugars, and the subsequent production of ethanol from this lignocellu-losic biomass. Using response surface methodology (RSM), the influence of US power, time, temperatureand biomass loading on fermentable sugar yield from BSG was studied. The optimal conditions werefound to be 20% US power, 60 min, 26.3°C, and 17.3% w/v of biomass in water. Under these conditions,an approximate 2.1-fold increase in reducing sugar yield (325 ± 6 mg/g of biomass) was achieved, relativeto untreated BSG (151.1 ± 10 mg/g of biomass). In …

Research And Innovation As A Catalyst For Food System Transformation, A.C.L. Den Boer, K.P.W. Kok, M. Gill, J. Breda, Jean Cahill, C. Callenius, P. Caron, Z. Damianova, M. Gurinovic, L. Lähteenmäki, T. Lang, R. Sonnino, G. Verburg, H. Westhoek, T. Cesuroglu, B.J. Regee, J. E. W. Broerse

Research and Innovation - Articles

Background: Food systems are associated with severe and persistent problems worldwide. Governance approaches aiming to foster sustainable transformation of food systems face several challenges due to the complex nature of food systems. Scope and Approach: In this commentary we argue that addressing these governance challenges requires the development and adoption of novel research and innovation (R&I) approaches that will provide evidence to inform food system transformation and will serve as catalysts for change. We first elaborate on the complexity of food systems (transformation) and stress the need to move beyond traditional linear R&I approaches to be able to respond to …

Anti-Hypochlorite, Antioxidant, And Catalytic Activity Of Three Polyphenol-Rich Super-Foods Investigated With The Use Of Coumarin-Based Sensors, Karolina Starzak, Tomasz Świergosz, Arkadiusz Matwijczuk, Bernadette Creaven, Janusz Podleśny, Dariusz Karcz

Articles

The anti-hypochlorite activity of açaí (Euterpe oleracea Mart.), goji (Lycium barbarum L.) and schisandra (Schisandra chinensis) fruit extracts were assessed by determining the reactive chlorine species (RCS)-scavenging ability of these three “super-food” berries. In addition, the aqueous extracts obtained were employed as both the media and the catalyst in a green chemistry approach to the synthesis of a coumarin-based fluorescence turn-off sensor, which was then used for anti-hypochlorite activity testing. The aqueous extracts were also assessed for total phenolic content (TPC), using the Folin–Ciocalteu method, and the antioxidant activity using the ABTS+• assay. Moreover, the main water-soluble polyphenolic constituents of …

Determining The Age Of Spoiled Milk From Dried Films Using Attenuated Reflection Fourier Transform Infrared (Atr Ft-Ir) Spectroscopy, Zack Richardson, David Perezguaita, Kamila Kochan, Bayden Wood

Articles

Milk spoilage is an inevitable occurrence, which generates waste and can result in food poisoning. When milk spoils, the off-flavor and curdling are due to excessive proliferation of various bacteria which causes pH changes. Time, temperature, environment,and previous handling practice all affects the spoilage rate. There is a need for a fast reliable and accurate method that can identify in situearly spoilage of milk. Here we show the ability of attenuated total reflectionFourier transformed infrared spectroscopy(ATR FT-IR) in conjunction with multivariate data analysis to predict the age of milk. We found that dried films vastly increased the absorbance of important …

Physicochemical Characterization, Structural Determination, And Molecular Dynamic Modeling Of Proso Millet Proteins For Enhanced Food Functionality, Felix Akharume

Theses and Dissertations--Biosystems and Agricultural Engineering

More than one-third of Americans today incorporate plant-based protein into their diet and about 40% believed that plant-based protein is healthier than animal protein, especially Millennials. The increasing global demand for plant-based proteins driven by the high cost of animal proteins, consumers’ desire for lean protein, vegetarianism, and the need for more sustainable green protein products have necessitated research into alternate emerging and underutilized sources of protein to complement or supplement the major plant protein in the market- soy, pea, and gluten. Therefore, this dissertation is focused on the valorization of the proteins in proso millet. Specifically, this work focused …

Dietary Fat Intakes In Irish Children: Changes Between 2005 And 2019, Aileen O’Connor, Maria Buffini, Anne Nugent, Laura Kehoe, Albert Flynn, Janette Walton, John Kearney, Breige Mcnulty

Articles

Objective:To examine current dietary fat intakes and compliance in Irish childrenand to examine changes in intakes from 2005 to 2019.Design:Analyses were based on data from the Irish National Children’s FoodSurvey (NCFS) and the NSFS II, two cross-sectional studies that collected detailedfood and beverage intake data through 7-day and 4-day weighed food diaries,respectively.Setting:NCFS and NCFS II, Republic of Ireland.Participants:A nationally representative sample of 594 (NCFS) and 600 (NCFS II)children aged 5–12 years. Current intakes from the NCFS II were compared withthose previously reported in the NCFS (www.iuna.net).Results:Current intakes of total fat, SFA, MUFA, PUFA andtransfat as a percentageof total energy are …

Associations Between The Home Environment, Feeding Practices And Children’S Intakes Of Fruit, Vegetables And Confectionary/Sugar-Sweetened Beverages, Carolina Bassu, Clare Corish, John Kearney

Articles

Within the home environment, parents influence their children’s dietary intakes through their parenting and dietary practices, and the foods they make available/accessible. The aim of this cross-sectional study was to examine the associations between home environmental characteristics and children’s dietary intakes. Three hundred and thirty-two children aged three–five years and their parents participated in the study. Home environmental characteristics, including parental control feeding practices, were explored using validated and standardized questionnaires such as the Child Feeding Questionnaire (CFQ), the Physical and Nutritional Home Environment Inventory (PNHEI) and the Healthy Home Survey (HHS). Parent and child food consumption was also measured. …

Computational Modelling Approach For The Optimization Of Apple Juice Clarification Using Immobilized Pectinase And Xylanase Enzymes, Shady Hassan, Gwilym Williams, Amit Jaiswal

Articles

Apple juice is typically marketed as a clear juice, and hence enzymatic treatments are common practices in juice industry. However, enzymatic treatments have been shown to face some challenges when process efficiency, and cost effectiveness are concerned. Therefore, it is necessary to optimize the enzymatic treatment process to maximize efficiency, and reuse enzymes to minimize the overall cost via immobilization. In this context, the present work features the immobilization of pectinase and xylanase from M. hiemalis on genipin-activated alginate beads, with subsequent evaluation of its efficacy in apple juice clarification. A central composite rotatable design (CCRD), coupled with artificial neural …

Salcaprozate Sodium (Snac) Enhances Permeability Of Octreotide Across Isolated Rat And Human Intestinal Epithelial Mucosae In Ussing Chambers, Sarinj Fattah, Mohamed Ismaiel, Brenda Murphy, Aleksandra Rulikowska, Jesus Maria Frias, Desmond C. Winter, David J. Brayden

Articles

Octreotide is approved as a one-month injectable for treatment of acromegaly and neuroendocrine tumours. Oral delivery of the octapeptide is a challenge due mainly to low intestinal epithelial permeability. The intestinal permeation enhancer (PE) salcaprozate sodium (SNAC) has Generally Regarded As Safe (GRAS) status and is a component of an approved oral peptide formulation. The purpose of the study was to examine the capacity of salcaprozate sodium (SNAC), to increase its permeability across isolated rat intestinal mucosae from five regions and across human colonic mucosae mounted in Ussing chambers. Apical-side buffers were Kreb's-Henseleit (KH), fasted simulated intestinal fluid (FaSSIF-V2), rat …

Temperature Stability And Effectiveness Of Plasma-Activated Liquids Over An 18 Months Period, Evanthia Tsoukou, Paula Bourke, Daniela Boehm

Articles

Non-buffered plasma-activated liquids such as water and saline have shown bactericidal effects. In the present study, we investigated the anti-bacterial efficacy and stability of plasma-activated water (PAW) and plasma-activated saline (PAS), generated using a high voltage dielectric barrier discharge system. This study compares the potential of non-buffered plasma-activated liquids (PAL) for the inactivation of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) after storage of the solutions at five different temperatures for a storage time up to 18 months after their generation. The temperatures used were room temperature, 4 °C, −16 °C, −80 °C, −150 °C. Both PAW and …

Food Security And Diet Quality In Native Hawaiian, Pacific Islander, And Filipino Infants 3 To 12 Months Of Age, Sally Campbell, John J. Chen, Carol J. Boushey, Heather Eicher-Miller, Fengqing Zhu, Marie K. Fialkowski

Articles

Food insecurity and other nutritional risks in infancy pose a lifelong risk to wellbeing; however, their effect on diet quality in Native Hawaiian, Pacific Islander, and Filipino (NHPIF) infants in Hawai‘i is unknown. In this cross-sectional analysis, the association between various indicators of food security and NHPIF infant diet quality were investigated in 70 NHPIF infants aged 3–12 months residing on O‘ahu, Hawai‘i. The dietary assessments of the infants were collected using a mobile food record^TM. Foods consumed across four days were categorized into seven food groups. Indicators for food security were examined through an adapted infant food …

Effect Of Atmospheric Cold Plasma On The Functional Properties Of Whole Wheat (Triticum Aestivum L.) Grain And Wheat Flour, Sonal Chaple, Chaitanya Sarangapani, John B. Jones, Ed Carey, Lucie Causeret, Annaik Genson, Brendan Duffy, Paula Bourke

Articles

Atmospheric cold plasma (ACP) has emerged as a novel processing technology, with demonstrated efficiencies in microbial inactivation. However, studies on the effects of ACP and potential to modify the functional properties of foods are sparse. The objective of this study is to determine the effect of ACP on physico-chemical and functional properties of wheat flour. In this study, both whole wheat grains and wheat flour were subjected to a dielectric barrier discharge (DBD) contained plasma reactor for a range of treatment times (5–30 min) at 80 kV. Plasma treatment increased the flour hydration properties of wheat flour. Rapid visco-analyser results …

Development Of A Selective Inhibitor For Kv1.1 Channels Prevalent In Demyelinated Nerves, Ahmed Al-Sabi, Declan Daly, Myles Rooney, Cian Hughes, Gemma K. Kinsella, Seshu Kaza, Kieran Nolan, J. Oliver Dolly

Articles

Members of the voltage-gated K+ channel subfamily (Kv1), involved in regulating transmission between neurons or to muscles, are associated with human diseases and, thus, putative targets for neurotherapeutics. This applies especially to those containing Kv1.1 α subunits which become prevalent in murine demyelinated axons and appear abnormally at inter-nodes, underlying the perturbed propagation of nerve signals. To overcome this dysfunction, akin to the consequential debilitation in multiple sclerosis (MS), small inhibitors were sought that are selective for the culpable hyper-polarising K+ currents. Herein, we report a new semi-podand – compound 3 – that was designed based on the modelling of …

What The Covid-19 Crisis Is Telling Humanity, David Wiebers, Valery Feigin

Animal Sentience

The planet is in a global health emergency exacting enormous medical and economic tolls. It is imperative for us as a society and species to focus and reflect deeply upon what this and other related human health crises are telling us about our role in these increasingly frequent events and about what we can do to prevent them in the future.

Cause: It is human behavior that is responsible for the vast majority of zoonotic diseases that jump the species barrier from animals to humans: (1) hunting, capture, and sale of wild animals for human consumption, particularly in live-animal markets; …

Plant-Based Diets And Covid-19: Those Who Harvest Crops Are At High Risk, Jarret S. Lovell

Animal Sentience

This commentary extends Wiebers & Feigin’s (2020) plea to adopt diets that are less dependent on animals by calling on experts and activists to work for change with regard to farm worker labor conditions. Already doing among the most dangerous jobs, farmworkers are at increased risk of COVID-19. As we increasingly transition to plant-based diets, we must all ensure that farmworkers have safe and just working conditions to meet the demands of our changing diets.