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Food Biotechnology Commons

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2019

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Full-Text Articles in Food Biotechnology

Study On Antioxidant And Antibacterial Activities Of Extracts From Different Polar Acalypha Australis L., Yin Xianlou, Zhan Jihua, Tan Yang, Pei Gang, Li Ling Jun 2019

Study On Antioxidant And Antibacterial Activities Of Extracts From Different Polar Acalypha Australis L., Yin Xianlou, Zhan Jihua, Tan Yang, Pei Gang, Li Ling

Food and Machinery

1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) method, β-carotene bleaching method and nitric oxide (NO) method were used to determine the antioxidant activities of different extracts from Acalypha australis L.; All the extracts were evaluated for their bacteriostatic effect using microporous turbidimetry method, and three gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa, Helicobacter pylori and four gram-positive bacteria: Staphylococcus epidermidis, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus were determined in our research. The results showed all the extracts we determined had the activities of better scavenging DPPH, NO free radicals and inhibition of β-carotene bleaching. At the concentration was 2.0 mg/mL, the n-butanol extract had the strongest …


The Effects Of Oxygen-Insulated Grinding Process On The Quality Of Yuba, Ji Qiuyan, Kong Xiangzhen, Hua Yufei Jun 2019

The Effects Of Oxygen-Insulated Grinding Process On The Quality Of Yuba, Ji Qiuyan, Kong Xiangzhen, Hua Yufei

Food and Machinery

Firstly, the soymilk was produced by oxygen-insulated and normal grinding processes. The basic components and protein components were test. Then the soymilk was used to make yuba. And the yield, components, color, elongation ratio and boiling fastness of two kinds of yuba were compared. There was no significant difference in the components and protein compositions of soybean milk produced by different grinding processes. Oxygen-insulated soybean milk had a low degree of oxidation according to the SDS-PAGE. The yield of the two soybean milk products was similar. The change trends of protein contents in different yuba were different, and the fat …


Study On Optimization Of Microwave Roasting Process And Fracture Force Relationship Of Arabica Coffee, Zhang Fujie, Wang Lu, Yang Wei, Li Mengli, Li Lixia, Jiang Kuaile, Li Xiaoqing Jun 2019

Study On Optimization Of Microwave Roasting Process And Fracture Force Relationship Of Arabica Coffee, Zhang Fujie, Wang Lu, Yang Wei, Li Mengli, Li Lixia, Jiang Kuaile, Li Xiaoqing

Food and Machinery

With the power density, roasting time, spinning speed of roller and wind speed of microwave as the experimental factors, and the bulk density and roasting color value of coffee beans as the indexes for evaluation, the single factor test and the four-factor four-level (44) orthogonal test were conducted. It was shown by the results of optimization that the optimal technology parameters were: the microwave power density 8 W/g, the roasting time 12 min, the speed of roller 70 r/min, and the wind speed 1.5 m/s. Through experiments, it was confirmed that the bulk density of microwave roasted coffee beans under …


Optimization Of Microwave Vacuum Drying Technology Of Yam And The Evaluation Of Its Functional Activity, Xia Lei, Huang Zhao, Ouyang Huafeng, Guo Shiyin, Su Xiaojun, Wang Feng, Li Qingming Jun 2019

Optimization Of Microwave Vacuum Drying Technology Of Yam And The Evaluation Of Its Functional Activity, Xia Lei, Huang Zhao, Ouyang Huafeng, Guo Shiyin, Su Xiaojun, Wang Feng, Li Qingming

Food and Machinery

With the polysaccharide content, whiteness and rehydration rate as evaluation indexes, the microwave vacuum drying process of yam was optimized by single factor and quadratic rotation orthogonal experiment, and the functional active components and antioxidant capacity of dried yam were evaluated. The results showed that the regression model established by quadratic rotation orthogonal experiment could reflect the relationship between factors and indexes appropriately. The optimum production conditions were found to be the 3 mm-thick slices were vacuumed with -0.08 MPa at 55 ℃. Under the control of these conditions, the polysaccharide content, whiteness and rehydration rate of dried yam were …


Study On The Processing Of Flower-Scented Green Tea With Fresh Tea Leaves In Summer, He Lin, Li Zongqiong, Zhang Tuo, Liang Zhuangxian, Zhu Jingjing, Xiao Wenjun Jun 2019

Study On The Processing Of Flower-Scented Green Tea With Fresh Tea Leaves In Summer, He Lin, Li Zongqiong, Zhang Tuo, Liang Zhuangxian, Zhu Jingjing, Xiao Wenjun

Food and Machinery

In this study, fresh tea leaves with one bud and one leaf in summer of BiXiangZao were used as raw materials. The rotation (sun withering, cooling, rotating, spreading) were integrated into the spreading process on the basis of traditional green tea processing technology. Through sensory evaluation, analysis of flavor and aroma quality components, the technology of “rotation” for making flower-scented green tea with leaves in summer was optimized and screened. The results showed that the summer green tea processed by the “rotation” technology of sun withering for 5 min, spreading for 0.5 h, sun withering for 5 min, spreading for …


Study On Sterilization Effect And Process Optimization Of Dried Bean Curd Bythermosonication Treatment, Wu Ni, Zhao Wuqi, Lu Dan, Zeng Xiangyuan, Zhang Qingan, Song Shujie, Liu Fan Jun 2019

Study On Sterilization Effect And Process Optimization Of Dried Bean Curd Bythermosonication Treatment, Wu Ni, Zhao Wuqi, Lu Dan, Zeng Xiangyuan, Zhang Qingan, Song Shujie, Liu Fan

Food and Machinery

In order to obtain the optimum process of thermosonication treatment(TST)sterilization of dried bean curd(DBC), on the basis of single factor, the ultrasonic power, ultrasonic time and temperature were selected as the factors, and the decreased logarithm value of bacteria, color difference ΔE*, L*, a*, b*, hardness, chewiness, resilience, springiness, cohesiveness, protein content were used as indicators, the three-factor box Benhnken test was designed and the characteristic index for evaluating the quality of the DBC was screened out by factor analysis, the model was established and the best parameters were optimized. Results: the …


Analysis Of Main Characteristics And Influencing Factors Of Chinese Liquor In International Export Market, Cheng Tieyuan Jun 2019

Analysis Of Main Characteristics And Influencing Factors Of Chinese Liquor In International Export Market, Cheng Tieyuan

Food and Machinery

Firstly, analyzes the current situation of Chinese liquor products export to the international market. It compares the relevant market of liquor export from the perspective of country, region in terms of amount, quantity and average price. Proposed the main characteristics of the international market of Chinese liquor export: the wide distribution of export market and the obvious differences among different markets, the concentration of export market still being dominated by the traditional Asian market, and the obvious periodicity of liquor export. The influencing factors of liquor export in China were analyzed: the relation with population quantity, GDP and GDP per …


Progress On Effects Of Plant Polyphenols On Intestinal Microecology, Li Hao, Peng Xiyang, Wu Paixuan, Dai Zhiyong, Wang Jiaqi, Zhan Yanhong, He Xiangli Jun 2019

Progress On Effects Of Plant Polyphenols On Intestinal Microecology, Li Hao, Peng Xiyang, Wu Paixuan, Dai Zhiyong, Wang Jiaqi, Zhan Yanhong, He Xiangli

Food and Machinery

This paper discussed the regulation mechanism of plantpolyphenols on intestinal microecology, summarized and analyzed the effects of different kinds of polyphenols and mixed polyphenols extracts on the change of intestinal flora community structure, and prospected the research direction of plant polyphenols and the development of related products in the future.


The Research Progress On The Factors Affecting The Quality Of Crayfish Products, He Jiang, Yi Mengyuan, Hao Tao, Yang Pinghong, Zhou Shunxiang Jun 2019

The Research Progress On The Factors Affecting The Quality Of Crayfish Products, He Jiang, Yi Mengyuan, Hao Tao, Yang Pinghong, Zhou Shunxiang

Food and Machinery

Crayfish (Procambarus clarkii) industry is rapidly developed in China, and product quality is the key effect to ensure the healthy development of the industry. In the present review, factors affecting the quality of crayfish products were summarized, from the whole process of “breeding-processing-storage”. In the process of aquaculture, the environment had a significant impact on the safety quality of crayfish, such as heavy metal pollution. In the process of processing, the cooking and flavoring process is the key to the formation of good sensory quality of crayfish products. And in the process of storage, storage temperature, pre-freezing and packaging methods, …


Design And Analysis Of A Parallel Multi-Fingered Dexterous Hand, Fand Yuefa, Zhou Siyuan May 2019

Design And Analysis Of A Parallel Multi-Fingered Dexterous Hand, Fand Yuefa, Zhou Siyuan

Food and Machinery

Aiming at the shortcomings of series dexterous hand, a new multi-fingered dexterous hand is designed and analyzed based on the advantage of parallel mechanism. Firstly, according to the method of configuration synthesis, a three-degree-of-freedom parallel finger mechanism is proposed and its kinematics is analyzed. Then, based on the configuration of finger mechanism, a suitable palm is designed and combined into a multi-fingered dexterous hand. Finally, the grasping ability and reachable workspace of the parallel multi-fingered dexterous hand are analyzed and compared with the performance of the series dexterous hand. The results show that the multi-fingered dexterous hand based on parallel …


Studies On The Rheological, Bubble Microstructure And Baking Properties Of The Low-Fat Cake With Propylene Glycol Esters, Wang Jiabao, Chen Cheng, Wang Feng, Hao Yuehui, Huang Jinxin, Chen Junmin, Huang Weining, Akihiro Ogawa May 2019

Studies On The Rheological, Bubble Microstructure And Baking Properties Of The Low-Fat Cake With Propylene Glycol Esters, Wang Jiabao, Chen Cheng, Wang Feng, Hao Yuehui, Huang Jinxin, Chen Junmin, Huang Weining, Akihiro Ogawa

Food and Machinery

This study was conducted to evaluate the effect of new propylene glycol esters emulsifier on the specific gravity, viscosity, rheology, microstructure of batter and baking characteristics of low-fat cake. The results showed that propylene glycol esters increased the surface tension of oil and promoted changes in fat structure to the α crystalline form as observed by XRD. The small bubbles were protected by propylene glycol esters from defoaming caused by oil. The number of bubbles increased by 1.87 times. Propylene glycol ester significantly increased the specific volume and decreased hardness of low-fat cake.


Effect Of Tea Polyphenols On Antioxidation And Starch Digestion Characteristics Of Black Powder, Liu Xin, He Xinyi, Wang Hongbin, Huang Zonghai, Liu Xiaodong, Zhen Runyimg May 2019

Effect Of Tea Polyphenols On Antioxidation And Starch Digestion Characteristics Of Black Powder, Liu Xin, He Xinyi, Wang Hongbin, Huang Zonghai, Liu Xiaodong, Zhen Runyimg

Food and Machinery

The effects of different tea polyphenols on the antioxidant and digestive properties of black powder were studied. Taking the DPPH scavenging free radical rate, total reducing power, starch hydrolysis curve as the indicators, discussed the effect of tea polyphenols on the antioxidant and digestive properties of black powder. Research indicates: the addition of tea polyphenols significantly changed the index of black powder (P<0.05), enhanced DPPH free radical scavenging ability, total reducing power, total antioxidant, reduced starch hydrolysis rate. When the amount of tea polyphenols added is 0.040%, the DPPH free radical scavenging ability, total reducing power absorbance and total antioxidant ability of black powdered powder increased by 4.6%, 0.58, 8.83 U/g, respectively. The starch hydrolysis rate of black powder was reduced by 24.98%.


The Flavor Compounds In Fermented Sausages Analyzed By Gc-Ms And Its Relationship With Fermentation Time, Li Jia, Liu Zhongyi, Fu Man, Liu Hongyan, Wu Xiaoyan, Li Ting May 2019

The Flavor Compounds In Fermented Sausages Analyzed By Gc-Ms And Its Relationship With Fermentation Time, Li Jia, Liu Zhongyi, Fu Man, Liu Hongyan, Wu Xiaoyan, Li Ting

Food and Machinery

The single factor test was used to analyze the influence of the three factors, sample mass, extraction time and extraction temperature, on the extraction effect of flavor substances from the fermented sausage. And the aroma components from sausages fermented for 6 h and 9 h at 32 ℃ were compared. The results showed that the optimized extraction conditions were the sample mass of 3.0 g when extracted for 50 min at 60 ℃. The 54 and 20 aroma components were respectively identified in sausages fermented for 6 h and 9 h. The esters were found the main volatile components in …


Design Of Robot Nc Cooperative Electromechanical System For Remote 3d Cake Printer, An Jiaqiang, Tang Xiaohua, Wu Jing, Yang Siping, Li Tianjiao May 2019

Design Of Robot Nc Cooperative Electromechanical System For Remote 3d Cake Printer, An Jiaqiang, Tang Xiaohua, Wu Jing, Yang Siping, Li Tianjiao

Food and Machinery

Based on the analysis of the forming law and manufacturing technology of butter cake, a new butter cake processing machine body was proposed, consisting of a three-degree-of-freedom manipulator and a four-axis motion platform. In the control system, ARM microcontroller and MCX514 motion control chip were used as the core hardware of the control system. At the software level, FreeRTOS embedded operating system and GRBL numerical control system were used as the software framework of the control system. Through the calibration of the basic coordinate system of the contact three-point handshake, the manipulator and the motion table were cooperated to complete …


Moisture Sorption Isotherm Characteristics And The Model Characterization Of Two-Components In The Rice-Made Wafer, Cheng Xueyu, Qian Yihan, Lu Lixin May 2019

Moisture Sorption Isotherm Characteristics And The Model Characterization Of Two-Components In The Rice-Made Wafer, Cheng Xueyu, Qian Yihan, Lu Lixin

Food and Machinery

The relation between the equilibrium moisture content and the water activity of crust filling at 23, 30 and 45 ℃ were studied by gravimetric method. The test data were fitted with some typical moisture absorption models by Matlab. The results showed that the moisture isotherm of crust and filling were both S type model. When the moisture activity was constant, the equilibrium moisture content first increase and then decrease with the increase of temperature, which was different from other foods. The Lewicki model and Peleg were the most suitable for fitting adsorption isotherms of crust and filling when the moisture …


Effect Of Ice Temperature Storage On Postharvest Grape Fruit Quality, Zhang Zhe, Zhang Qiuyue, Wang Huaiwen, Guo Xu, Zhang Ping, Zhu Zhiqiang, Tian Jinjin, Wang Sasa May 2019

Effect Of Ice Temperature Storage On Postharvest Grape Fruit Quality, Zhang Zhe, Zhang Qiuyue, Wang Huaiwen, Guo Xu, Zhang Ping, Zhu Zhiqiang, Tian Jinjin, Wang Sasa

Food and Machinery

In order to clarify the effect of ice-temperature storage on the quality of grape fruit, the quality of Italian grape was studied under four different storage temperatures: super-ice temperature (-3 ℃ and -2 ℃), ice temperature (-1 ℃) and ordinary low temperature (4 ℃). The results showed that the contents of chlorophyll, titratable acid and vitamin C of Italian grapes stored at -3 ℃ were relatively high, which could maintain the nutritional components of grapes well and help grapes to maintain better color and taste during storage.


Ozone Ice Preparation Technology And Its Research Advances In Food Preservation, Yuan Chenghao, Liu Yongle, Huang Yiqun, Wang Jianhui, Huang Shouen, Li Xianghong, Wang Faxiang, Yu Jian May 2019

Ozone Ice Preparation Technology And Its Research Advances In Food Preservation, Yuan Chenghao, Liu Yongle, Huang Yiqun, Wang Jianhui, Huang Shouen, Li Xianghong, Wang Faxiang, Yu Jian

Food and Machinery

Compared with the ozone gas and water, the ozone ice is easy to store and has a long half-life. Furthermore, ozone stored in the ice will be slowly released with the melting process of ice. Due to these precise characters, ozone ice can be used for long-term sterilization for food preservation. Starting from the progress of ozone production technology, the authors introduce the production of ozone, and the preparation of ozone water, and the application of ozone ice in food preservation.


Research Progress On The Bioactive Compounds And Their Bioactivities Of Pepper Seed, Zhong Jin, Liu Yida, Xiao Yu May 2019

Research Progress On The Bioactive Compounds And Their Bioactivities Of Pepper Seed, Zhong Jin, Liu Yida, Xiao Yu

Food and Machinery

In this revtew, the current research progress on the main nutritional components, bioactive substances and biological activities of pepper seed worldwide were summarized, to provide reference basis and new ideas for its comprehensive utilization.


Effects Of Four Kinds Of Functional Oligosaccharides On The Batter And The Baking Quality Of Sponge Cake, Wang Yongjun, Deng Wenting, Zheng Jianxian, Huang Weining May 2019

Effects Of Four Kinds Of Functional Oligosaccharides On The Batter And The Baking Quality Of Sponge Cake, Wang Yongjun, Deng Wenting, Zheng Jianxian, Huang Weining

Food and Machinery

To avoid various issues brought by high-sugar intake from sponge cake, we explored the feasibility of completely replacing sucrose in sponge cake with four kinds of functional oligosaccharides, whose effects on the batter and the baking quality were compared with each other. The results showed that the batters of isomaltooligosaccharide, fructooligosaccharide, and galactooligosaccharide were similar to that of the sucrose group, illustrating promises of introducing healthier formula without sacrificing product quality. The sponge cakes of the three substitute candidates maintained good taste after three-day storage. However, the fourth candidate, inulin, was too strong in water absorption, resulting in low quality …


Screening And Identification Of Lactic Acid Bacteria And Yeasts And Its Application In Fermented Rice Cake, Li Meilun, Jiang Mengyi, Gong Chuanjie, He Li May 2019

Screening And Identification Of Lactic Acid Bacteria And Yeasts And Its Application In Fermented Rice Cake, Li Meilun, Jiang Mengyi, Gong Chuanjie, He Li

Food and Machinery

In order to obtain starter cultures with good performance for fermented rice cake, lactic acid bacteria strains and yeast strains were isolated from traditional fermented rice slurry. Through the investigation of colonies, morphological characteristics and growth properties one strain of lactic acid bacteria and two strains of yeasts were screened. According to the 16S rDNA and 26S rDNA sequence analysis, they were identifided as Lactobacillus plantarum, Candida humilis and Saccharomyces cerevisiae. Remixing three superior fermentation strains in fermented rice cake, the optimum bacteria ratio was determined by analyzing the sensory quality and texture characteristics of the fermented products. The results …


Screening Of Sugarcane Brewing Yeast, Ying Jing, You Ling, Feng Xueyu, Wang Tao, Qiu Shuyi May 2019

Screening Of Sugarcane Brewing Yeast, Ying Jing, You Ling, Feng Xueyu, Wang Tao, Qiu Shuyi

Food and Machinery

In order to screen yeast strains suitable for brewing sugarcane wine, the commonly used commercial fruit wine yeast strains KD, DV10, Q23, EC1118, Angel yeast and H7Y7 were selected as the tested yeast strains. Sugarcane wine was fermented by using sugarcane Chuanzhe 17 as raw material, and the sugar consumption, alcohol content and clarity of the fermentation liquid were analyzed. By comparing the fermentation characteristics of different yeasts found that EC1118 had the strongest sugar consumption and wine production capacity, and it also had the lowest alcohol production. The H7Y7 strain fermented sugarcane wine with the highest ester content, which …


Breeding Of Saccharomyces Cerevisiae High-Yield Of Alcohol And Acid By Atmospheric Room Temperature Plasma, Wang Liye, Wang Haocheng, Ma Shan, Liu Weibing, Li Zehan, Song Jingjing, Yang Huafeng, Zhang Ying, Wu Yun May 2019

Breeding Of Saccharomyces Cerevisiae High-Yield Of Alcohol And Acid By Atmospheric Room Temperature Plasma, Wang Liye, Wang Haocheng, Ma Shan, Liu Weibing, Li Zehan, Song Jingjing, Yang Huafeng, Zhang Ying, Wu Yun

Food and Machinery

The Y6 strain screened from the grape surface was subjected to atmospheric pressure room temperature plasma (ARTP) mutagenesis, and the Saccharomyces cerevisiae strain with strong alcohol production ability, high acid production ability and stable fermentation performance were selected. The single-factor test confirmed that the optimal mutagenesis condition was lethality rate at 98.70% treated for 100 s. The target strain Y6-8 producing alcohol and producing acid was screened by 2,3,5-triphenyltetrazolium chloride (TTC) method, bromocresol green medium method and Du's tubule, and then its genetic stability was studied. The results showed that the mutagenized strain Y6-8 was …


Study On The Quality And Aroma Components Of Musalais By Different Treatments, Gao Xinyu, Bai Yujia, Zheng Wancai, Chen Lihui, Fan Shihao, Feng Zuoshan May 2019

Study On The Quality And Aroma Components Of Musalais By Different Treatments, Gao Xinyu, Bai Yujia, Zheng Wancai, Chen Lihui, Fan Shihao, Feng Zuoshan

Food and Machinery

Comparison of musalais wine by two concentration methods and the addition of supplementary materials. The quality and aroma components of four kinds of Musalais were studied and analyzed by QDA. The results showed that the total sugar, reducing sugar, soluble solids, pH, clarity, and bioactivity of frozen concentration group were significantly higher than those of the heat concentration group. Among the four kinds of Musalais, esters were the most abundant, up to 65 kinds of aroma-producing substances. The relative content of esters in frozen concentrated Musalais was the highest, about 48.77% of the total volatile substances. The relative content of …


Structure Analysis Of Whole Wheat Yeast Bacteria And Its Effects On Flavor Substances, Shang Ying, Xiong Rongyuan May 2019

Structure Analysis Of Whole Wheat Yeast Bacteria And Its Effects On Flavor Substances, Shang Ying, Xiong Rongyuan

Food and Machinery

In order to develop and improve the utilization of whole grains, the macrogenomics method was used to analyze the structural composition of the whole wheat traditional fermentative dough, and the result shows that the core fermentation genus was Lactobacillaceae Lactobacillus brevis. According to the relevant literature, the flavor substances of whole wheat traditional fermentative dough and commercial fermentative dough were determined. The traditional fermentative dough contains 9 more flavor substances than commercial fermentation and there were also differences in the content, with the hydrocarbon content was showing the highest. It was indicated that the traditional fermentation strain was more conducive …


Effects Of Roasted Temperature To Seed Processing On The Keyaroma Components And Sensory Quality Of Camellia Oil, Zhu Xiaoyang, Long Qizhi, Zhong Haiyan May 2019

Effects Of Roasted Temperature To Seed Processing On The Keyaroma Components And Sensory Quality Of Camellia Oil, Zhu Xiaoyang, Long Qizhi, Zhong Haiyan

Food and Machinery

In order to analyze the effect of roasted temperature to seed processing on the key aroma components and sensory attributes of camellia oil, compared correlations and differences between aroma components and sensory attributes of hot pressed camellia oil from Hainan and Hunan province, and the volatile compounds of these camellia oils were analyzed by HS-SPME-GC-MS. The ROAV value was calculated to determine the key aroma components. Sensory attributes were analyzed by sensory evaluation and the PLS model was established. The results showed that the most important flavor components in Hainan camellia oil and roasted seed samples of Hunan were (E)-2-nonenal, …


Effects Of Coercion Germination With Aeration And Metal Irons On The Enrichment Of Γ-Aminobutyric Acid In Germinated Brown Rice, Zhang Ying, He Jian, Wang Tao, Wang Ren, Chen Zhengxing May 2019

Effects Of Coercion Germination With Aeration And Metal Irons On The Enrichment Of Γ-Aminobutyric Acid In Germinated Brown Rice, Zhang Ying, He Jian, Wang Tao, Wang Ren, Chen Zhengxing

Food and Machinery

In order to shorten the germination cycle of brown rice and increase the content of γ-aminobutyric acid (GABA), the effects of aeration coercion, metal ion coercion and double stress treatment on brown rice (Zhen waxy rice 19) were studied. The effects of the content, germination rate and bud length showed that the aeration treatment during the whole germination process can significantly accelerate the germination rate of brown rice and increase the content of GABA in brown rice in the late germination stage. However, it would lead to the buds being too long. Aeration treatment for 9 h can effectively control …


Development Of Indirect Competitive Elisa For The Detection Of Dibutyl Phthalate In Food, Shang Shuna, Sheng Wei, Wang Lulu, Zhao Qiuxia, Wang Shuo May 2019

Development Of Indirect Competitive Elisa For The Detection Of Dibutyl Phthalate In Food, Shang Shuna, Sheng Wei, Wang Lulu, Zhao Qiuxia, Wang Shuo

Food and Machinery

In this study, 4-aminophthalic acid was used to synthesize hapten Dibutyl 4-aminophthalate by esterification reaction, and the synthesized hapten was coupled with keyhole limpet hemocyanin (KLH) by diazotization reaction to prepare the immune antigen and coupled with bovine serum albumin (BSA) to prepare the coating antigen. The New Zealand white rabbits were immunized to obtain the antiserum against dibutyl phthalate. An indirect competitive enzyme-linked immunosorbent assay (ELISA) for dibutyl phthalate was established using the purified antibodies. For the developed ELISA, the IC50 was 40.68 μg/L and the limit of detection was 1.98 μg/L. The method was applied to detect …


Rapid Detection Of Imidan In Apple Juice With Surface Enhanced Raman Spectroscopy, Xu Nianwei, Huang Yiqun, Lai Keqiang May 2019

Rapid Detection Of Imidan In Apple Juice With Surface Enhanced Raman Spectroscopy, Xu Nianwei, Huang Yiqun, Lai Keqiang

Food and Machinery

Au-Ag nanoparticles (NPs) varying in size (35~91 nm, Au core 19 nm; 66~127 nm, Au core 43 nm) were synthesized via a typical seed growth method and characterized for their morphology and optical properties. The Au-Ag NPs were then applied as surface-enhanced Raman scattering (SERS) substrates for imidan analysis and the influences of particle size and gold-silver ratio for SERS detection were investigated. The results showed that 42 nm Au-Ag NPs with 19 nm Au core and 78 nm Au-Ag NPs with 43 nm Au core had the highest SERS enhancement effect for imidan standard solutions, and could be used …


Rapid Detection Method Of Sausage Nitrite Based On Hyperspectral Technology, Liu Zheng, Yin Yong May 2019

Rapid Detection Method Of Sausage Nitrite Based On Hyperspectral Technology, Liu Zheng, Yin Yong

Food and Machinery

Seven different storage period sausages were selected for nitrite content detection and corresponding spectral data collection,and uses Savitzky-Golary method to preprocess spectral data to reduce the noise of spectral data. Then based on the pre-processed spectral data, 29 characteristic wavelengths were extracted by partial least squares regression coefficient method. Finally, the detection accuracy of the prediction model of nitrite in sausages at characteristic wavelength and full wavelength were analyzed. The results showed that the prediction results of the regression model based on full wavelength were all higher than that based on characteristic wavelength, and the full-wavelength partial least squares regression …


Study On Rapid Determination Of Tobacco Blending Uniformity By Near Infrared Spectroscopy, Li Ruili, Liu Yuye, Li Wenwei, Li Tiancheng, Wang Jianmin May 2019

Study On Rapid Determination Of Tobacco Blending Uniformity By Near Infrared Spectroscopy, Li Ruili, Liu Yuye, Li Wenwei, Li Tiancheng, Wang Jianmin

Food and Machinery

Based on the principal component analysis, we established F score statistics to reflect comprehensive information about main chemical composition and their ratio and used F score as the characteristic value to characterize the homogeneity of formula tobacco. We detected the near-infrared spectral of tobacco samples with different formula tobacco and founded the near-infrared spectral model of F score by using the partial least square method based on the range of 4 281.2~4 485.6,5 542.4~6 024.5,6 043.8~7 135.3 cm-1 spectroscopy pretreated by multiplicative signal correction and first-order derivative and Savitzkyt-Golay smoothing. The determinant coefficient of F score model was 0.996, …