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Articles 151 - 180 of 531
Full-Text Articles in Food Biotechnology
Effect Of Treatment On The Elimination Of Deoxynivalenol By Rho18, Yu Xiaofan, Zhang Chen, Xu Huiqing, Ding Xiangli, Yuan Yaming
Effect Of Treatment On The Elimination Of Deoxynivalenol By Rho18, Yu Xiaofan, Zhang Chen, Xu Huiqing, Ding Xiangli, Yuan Yaming
Food and Machinery
The effect of Rho18 treated with different methods on the removal of deoxynivalenol (DON) from wheat flour was determined by ELISA and HPLC-MS. Processing results showed that the different ways of rice root mildew Rho18 clearance to DON in wheat flour were all increased with the concentration increase with the extension of incubation time, as well as the high concentration of the treatment group. After 10 h incubation with the rest of the concentration of treatment group were significantly different, the cell walls of a high concentration of suspension and wheat 10 h incubation conditions to DON removal effect is …
Preparation Of Otc Complete Antigen And Establishment Of Elisa Detection Method, Cao Jinbo, Hu Xiaofei, Wang Yao, Li Yanhong, Cao Xidan, Xing Yunrui, Sun Yaning, Deng Ruiguang, Zhang Gaiping
Preparation Of Otc Complete Antigen And Establishment Of Elisa Detection Method, Cao Jinbo, Hu Xiaofei, Wang Yao, Li Yanhong, Cao Xidan, Xing Yunrui, Sun Yaning, Deng Ruiguang, Zhang Gaiping
Food and Machinery
To prepare complete antigen of oxytetracycline (OTC) and obtain mouse polyclonal antiserum with good immunological characteristics and establish immunological detection method of OTC. In this study, carboxyl groups were introduced into OTC molecules by diazotization, and then complete antigens were prepared by coupling with carrier proteins using an improved carbodiimine method. The coupling effect of complete antigen was identified by IR, UV and SDS-PAGE. Then the BLAB/c mice were immunized with complete antigens to obtain antiserum(pAb) and identify the antiserum immunological characteristics. After optimizing the reaction conditions of ELISA, indirect competitive ELISA method of OTC was established. The results showed …
Evaluation Of Uncertainty For The Determination Of Pentachlorophenol In Pork Liver By Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry, Ouyang Li, Wang Hui, He Yi, Chen Yaya, Hu Tingshu, Liu Manshu
Evaluation Of Uncertainty For The Determination Of Pentachlorophenol In Pork Liver By Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry, Ouyang Li, Wang Hui, He Yi, Chen Yaya, Hu Tingshu, Liu Manshu
Food and Machinery
Based on GB 23200.92-2016, the samples were detected, and a mathematical model was established. Moreover, each uncertainty of the whole analysis procedures was analyzed and quantified. It was found that, when the pentachlorophenol residue was 14 μg/kg, the extended uncertainty was 3 μg/kg (k=2) under the 95% confidence interval. The results showed that the main sources of measurement uncertainties are derived from the final volume of sample, measurement repeatability, standard solution preparation, and the calibration fitting.
Study On Identification Of Fritillariae Cirrhosae Bulbus By Improved Alkali Pyrolysis, Chen Yuxiu, Peng Biwu, Li Yuyan
Study On Identification Of Fritillariae Cirrhosae Bulbus By Improved Alkali Pyrolysis, Chen Yuxiu, Peng Biwu, Li Yuyan
Food and Machinery
The DNA of Fritillariae cirrhosae bulbus was extracted by alkali lysis. The effects of different proportion of lysate on DNA quality and electrophoretic success rate were investigated, which compared with the current methods. Results, The alkaline lysis buffer had the best effect on DNA extraction, which was made of 200 mmol/L NaCl, 5 mmol/L Mg2Cl, 1% Triton X-100, 1% PVP, 1% SDS, 2 mmol/L EDTA and 0.1 mol/L Tris-HCl. The DNA electrophoretic bands extracted by this alkali lysate were in accordance with the current method. The method can save 15~20 minutes, and the cost of single sample testing …
Isolation, Purification And Identification Of Microorganisms In Moldy Cigarettes Products, Lei Sheng, Yang Qianxu, Shi Yanping, Jiang Juxing, Liu Xiuming, Zhang Ling, Chen Wenyi, Wang Xuejiao, Zeng Yicheng
Isolation, Purification And Identification Of Microorganisms In Moldy Cigarettes Products, Lei Sheng, Yang Qianxu, Shi Yanping, Jiang Juxing, Liu Xiuming, Zhang Ling, Chen Wenyi, Wang Xuejiao, Zeng Yicheng
Food and Machinery
Five strains were isolated and purified from moldy cigarettes, and were identified by fungal morphology and microscopy. It was found that there were 2 strains of Aspergillus, 1 strain of Penicillium, 1 strain of Alternaria, and 1 strain of Cladosporium. Through the 18S rDNA-ITS sequence analysis and the construction of phylogenetic tree, the mildew microorganism of cigarettes were identified as five species, such as Aspergillus sydowii, Aspergillus flavus, Cladosporiumtenuissimum, Penicilliumdigitatum and Alternaria. It was concluded that Aspergillus flavus was the main strain causing mildew in cigarette products by the mildew experiment.
The Exchange Mechanism Of Food Safety Risks In Japan, Europe And America And Its Enlightenment, Gu Kaichen, Chang Zhirong, Wei Ting, Yao Xiaoyuan
The Exchange Mechanism Of Food Safety Risks In Japan, Europe And America And Its Enlightenment, Gu Kaichen, Chang Zhirong, Wei Ting, Yao Xiaoyuan
Food and Machinery
Building risk communicationsystem is one of the most important means to prevent and manage food problems in the field of international food safety. It has become the consensus of all countries to apply it to the management of food safety. This paper systematically expounds the mechanism of food safety risk communication in Japan, EU and USA, and analyses its framework and functions, aiming at exploring the mechanism of food safety risk communication in foreign countries, and providing enlightenment for the establishment and implementation of the mechanism of food safety risk communication in China.
Path Dimension Of Industry Self-Discipline In Tourism Food Safety Governance, Han Peng
Path Dimension Of Industry Self-Discipline In Tourism Food Safety Governance, Han Peng
Food and Machinery
The industry self-discipline management of tourism industry associations is very important to the improvement of the efficiency of tourism food safety management. However, in the practice of self-discipline management, lacked the relevant systems to regulate industry self-discipline management, specialized professionals and institutions of industry self-discipline management, as well as the coordination system between government supervision and industry self-regulation. The inherent mechanism of these problems was that the particularity of tourism food safety management was not given necessary attention, the professionalism of tourism food safety management was neglected artificially, and the limitation of government supervision ability was not correctly recognized. Therefore, …
Experimental Study On R404a/R23 Cascade Ultra-Low Temperature Refrigeration System, Liu Han, Xie Jing, Wang Jinfeng
Experimental Study On R404a/R23 Cascade Ultra-Low Temperature Refrigeration System, Liu Han, Xie Jing, Wang Jinfeng
Food and Machinery
Relying on the R404A/R23 cascade ultra-low temperature refrigeration test bench, and using the method of controlling single variable, the evaporation temperature and condensation temperature of the compressor compression ratio, exhaust gas temperature, suction pressure and refrigerant flow rate in the cascade refrigeration cycle were studied. The effect of the refrigeration coefficient. The results showed that when the evaporation temperature was reduced from -50 ℃ to -55 ℃, the system refrigeration coefficient declined the fastest, reaching 2.1%; when the condensation temperature was raised from 28 ℃ to 30 ℃, the system refrigeration coefficient declined the fastest, with 1.7%. And the evaporation …
Numerical Simulation And Optimization Of Starchclassification In Turbo Air Classifiers, Lou Qi, Zhao Jiejun, Yu Jianfeng
Numerical Simulation And Optimization Of Starchclassification In Turbo Air Classifiers, Lou Qi, Zhao Jiejun, Yu Jianfeng
Food and Machinery
The ICEM software was used to mesh the model of turbo air classifier and numerical simulation was performed by Fluent software to characterize separation process of potato starch. The effect of parameters such as rotational speed, inlet velocity and number of rotor blades on the cut size were studied. The response surface analysis method was used to optimize process parameters on turbo air classifier. The results indicated that the rotational speed, inlet velocity and number of rotor blades all influenced the cut size of potato starch. The order of effect on cut size was as followed: rotational speed＞inlet velocity＞number of …
Optimization Design Of Flexible Pneumatic Separation System Adapted To The Processing Characteristics Of Slim Cigarettes, Yuan Haixia, Zheng Xi, Zhang Shenghua, Xia Zilong, Lan Zhichao, Li Jun, Wang Shanshan, Guan Aizhang, Zeng Jing, Zhang Kai
Optimization Design Of Flexible Pneumatic Separation System Adapted To The Processing Characteristics Of Slim Cigarettes, Yuan Haixia, Zheng Xi, Zhang Shenghua, Xia Zilong, Lan Zhichao, Li Jun, Wang Shanshan, Guan Aizhang, Zeng Jing, Zhang Kai
Food and Machinery
To reduce the stem sliver content in cut tobacco of slim cigarettes and the cut tobacco content in online removed stem sliver, improve cut tobacco size distribution, and enhance product quality and its stability of slim cigarettes, a three-stage flexible pneumatic separation system was designed adapting to high-flow pneumatic separation of cut lamina of slim cigarettes based on the two-stage pneumatic separation system of cut lamina. The system was mainly made up of input unit, loosening roller, three-stage pneumatic separation tanks, cyclone blanker (settling blanker), transportation unit, output unit, dust extraction pipe and other parts. Its first and second stage …
“Hong Kong Red” Red Wine Brand Shaping Andpackaging Design Based On Fengshui Cultural, Qu Yundong, Mao Han, Zhu Li
“Hong Kong Red” Red Wine Brand Shaping Andpackaging Design Based On Fengshui Cultural, Qu Yundong, Mao Han, Zhu Li
Food and Machinery
In view of the current Chinese red wine packaging design lack of national cultural identity, positioning is unclear, brand characteristics weakened and other phenomena. Explore the concept of fengshui, fengshui keeping in good health and fengshui aesthetics in Chinese traditional fengshui culture, and its inheritance and use on the “Hong Kong red” wine brand shaping and packaging design，the purpose is to create the “fengshui red wine” series of products. In trying to bring health and good luck of people's daily life, at the same time, realize their own “good fengshui, good life” management value idea.
The Research On The Packaging Design Of Kazakh Traditional Food, Qin Weiwei
The Research On The Packaging Design Of Kazakh Traditional Food, Qin Weiwei
Food and Machinery
Starting from the Kazakh traditional wooden receptacles, the shapes, patterns and colors with ethnic symbols are used to transform the Kazakh traditional food packaging design, forming a unique packaging language and visual characteristics, in order to promote the promotion of traditional food.
Effect Of Pre-Cooling Conditions On Fresh-Keeping To Ice-Temperature Tilapia Fillets, Jin Zhi, Guan Zhiqiang, Lin Min
Effect Of Pre-Cooling Conditions On Fresh-Keeping To Ice-Temperature Tilapia Fillets, Jin Zhi, Guan Zhiqiang, Lin Min
Food and Machinery
Pre-cooling treatment was introduced before the storage of fish fillets at ice temperature, and the effects of different temperature pretreatment conditions on the quality of tilapia fillets during ice storage were investigated. The tilapia fillets were pre-cooled by setting different pretreatment temperatures (-18, -30, -60 ℃), and the center temperature of the fillets was lowered to -1 ℃, and then stored in -2 ℃. The pH, color, texture, volatile base nitrogen (TVB-N), total number of colonies, thiobarbituric acid value (TBA), and calcium ionize activity (Ca2+-ATPase) were used as indicators to investigate the time interval. The next series of …
Analysis Of Quality And Colony Diversity Of Products Of Crayfish Under Different Storage Temperatures, Zhou Tao, Wu Xiaoying, Luo Haibo, Man Wenzeng, Wang Rui, Wan Chenyue
Analysis Of Quality And Colony Diversity Of Products Of Crayfish Under Different Storage Temperatures, Zhou Tao, Wu Xiaoying, Luo Haibo, Man Wenzeng, Wang Rui, Wan Chenyue
Food and Machinery
In this study, the quality and dominant spoilage flora in products of crayfish during 4 and 25 ℃ storage were studied. The shelf life was determined by measure its physical and chemical indicators. Microbial genomic DNA was extracted from the collected samples, and the changing trend of microbial community was analyzed by high-throughput sequencing technology. Results showed that, with the extension of storage time, species diversity decreased gradually. The dominant flora at 4 ℃ were Oceanobacillus (81.522%) and Shewanella (3.625%). The dominant flora at 25 ℃ were Hathewaya (78.700%), Bacillus (12.380%) and Clostridium sensu stricto (8.707%). This study provided a …
Modeling Of Moisture Diffusion In Two Components Food Of Rice Made Wafer With Impermeable Package, Cheng Xueyu, Qian Yihan, Lu Lixin
Modeling Of Moisture Diffusion In Two Components Food Of Rice Made Wafer With Impermeable Package, Cheng Xueyu, Qian Yihan, Lu Lixin
Food and Machinery
In this study, a moisture diffusion model of two-component food rice made wafer under a non-permeable packaging condition was established on the basis of water adsorption kinetics experiment and water diffusion coefficient theory under the condition of 23 ℃; The relation between water content and time change of water diffusion among components was calculated based on the Fick’s law, initial conditions, boundary conditions and moisture sorption isotherm, and then the model-calculated of moisture diffusion were tested and analyzed by experiment when the moisture content was between 0.02 and 0.12. The results showed that the experimental data could be tted with …
Ultrasonic-Assisted Preparation, Stability, And Reducing Blood Lipid Of Fish Oil Microcapsule From Grass Carp (Ctenopharyngodon Idella), Liu Xiaoli, Wei Changqing, Zhan Xiaobei, Xia Wenshui
Ultrasonic-Assisted Preparation, Stability, And Reducing Blood Lipid Of Fish Oil Microcapsule From Grass Carp (Ctenopharyngodon Idella), Liu Xiaoli, Wei Changqing, Zhan Xiaobei, Xia Wenshui
Food and Machinery
With grass carp (Ctenopharyngodon idella) oil used as core material, chitosan (CTS) and soy protein isolate (SPI) as wall materials, CTS/SPI fish oil microcapsule product was prepared by use ultrasonic and spray drying. The embedding rate, particle size, thermal stability, storage stability, fish oil and microcapsule functional fatty acids content changes before and after storage and fall hematic fat action were studied. The results showed that the preparation of microcapsule embedding rate reached 77%, with the average particle size 15.8 μm, and the glass transition temperature (Tg) was 71 ℃. The functional fatty acids content changed little at 60 ℃, …
Antagonistic Effect Of Camphor Leaf Essential Oil On Damage Induced By Pm2.5 In Lung Epithelial Cells A549, Xiong Guohong, Lin Hai, He Jianguo, Li Yinghong, Fu Xiangjin
Antagonistic Effect Of Camphor Leaf Essential Oil On Damage Induced By Pm2.5 In Lung Epithelial Cells A549, Xiong Guohong, Lin Hai, He Jianguo, Li Yinghong, Fu Xiangjin
Food and Machinery
In this paper, the lung cell A549 was used as a model. The protective effect of camphor (C. camphora) leaf essential oil on the A549 exposed to PM2.5 was investigated. Less than 1 mg/mL of essential oil had no effect on A549 cell proliferation, and low concentration had a certain promotion effect. 0.05～1.00 mg/mL essential oil had a significant antagonistic effect on cytotoxicity induced by PM2.5 (P＜0.05). The essential oilsignificantly reduced the content of MDA in the infected cells and increased the activity of SOD and CAT and the content of T-AOC (P＜0.05), indicating that the camphor leaf essential oil …
Optimization On Extracting Process For Red Meat Pitaya Pigment Assisted With Enzymatic-Ultrasonic By Response Surface Methodology, Tian Yan, Duan Xiaoyan, Deng Fangming
Optimization On Extracting Process For Red Meat Pitaya Pigment Assisted With Enzymatic-Ultrasonic By Response Surface Methodology, Tian Yan, Duan Xiaoyan, Deng Fangming
Food and Machinery
Using freeze-dried pitaya peel and pulp samples as raw materials, response surface methodology was used to optimize the cellulase-ultrasonic extraction process of pigment from pitaya peel and pulp. The optimal extraction process of pigment from pitaya pericarp was as followed: extraction time 24 min, extraction temperature 39 ℃, ultrasonic power 325 W, ethanol concentration 23%, the yield was (81.661 3±0.762 0) mg/100 g; The optimum extraction conditions of pigment from pitaya pulp were as follows: extraction time 34 min, extraction temperature 41 ℃, ultrasonic power 315 W, ethanol concentration 23%, and the yield was (278.660 4±1.084 2) mg/100 g.
Study On Physicochemical And Sensory Properties, Polyphenolic Composition And Antioxidant Capacity Of Matcha Tea Infusion, Zhang Qiqi, Cheng Junxia, Wang Qi, Wang Haopeng, Yang Na, Guo Juntong, Shao Hongjun
Study On Physicochemical And Sensory Properties, Polyphenolic Composition And Antioxidant Capacity Of Matcha Tea Infusion, Zhang Qiqi, Cheng Junxia, Wang Qi, Wang Haopeng, Yang Na, Guo Juntong, Shao Hongjun
Food and Machinery
The physicochemical and sensory properties, polyphenolic composition, and antioxidant capacity of two kinds of matcha tea infusion were investigated in the present study. The results showed that the pH increased slightly, and the titratable acid content decreased dramatically from the first to the third brew. Meanwhile, the infusions of the first brew had optimal color and scent sensory quality compared with those of the second and third brews. Furthermore, the total polyphenol content, polyphenolic composition, and antioxidant capacity of tea infusions showed no significant difference between the two kinds of matcha tea, but all decreased to some degrees during the …
Study On Two-Stage Partial Block Freeze Concentration To Prepare High Content Apple Cider Vinegar, Li Xiangqin, Zhong Ruimin, Men Geyang, Shan Bin, Liao Caihu, Zhong Pingjuan, Wu Lanjie, Huang Zuanyuan
Study On Two-Stage Partial Block Freeze Concentration To Prepare High Content Apple Cider Vinegar, Li Xiangqin, Zhong Ruimin, Men Geyang, Shan Bin, Liao Caihu, Zhong Pingjuan, Wu Lanjie, Huang Zuanyuan
Food and Machinery
Used the self-developed 50 L progressive partial block freeze concentration pilot equipment to concentrate the soluble solid content of 5.0 °Brix apple cider vinegar. By adjusting the stirring speed and the cold medium temperature, comprehensive energy consumption, time and other factors, explored the optimum technological conditions of the two-stage and recycle liquid entrainment effectively. And at the same time, used solid-phase micro extraction-gas chromatography mass spectrometry to explore the changes of aromatic substances in the difference of apple original vinegar, all levels of concentrated compound liquid, two-stage entrainment solution, entrainment recovery solution and ultimate entrainment. Results: The apple cider vinegar …
Study On The Suitability Of Fresh Tea Leaves From Different Teavarieties For Yellow Tea Processing, Song Yuxin, Zhu Jingjing, Zhang Tuo, Gong Zhihua, Xiao Wenjun
Study On The Suitability Of Fresh Tea Leaves From Different Teavarieties For Yellow Tea Processing, Song Yuxin, Zhu Jingjing, Zhang Tuo, Gong Zhihua, Xiao Wenjun
Food and Machinery
Taking the tea varieties of Bixiangzao, Jianbohuang and Taoyuandaye in summer with a bud and one leaf as raw materials, which were processed into yellow tea, respectively. The yellow tea suitability of three tea varieties was discussed via analyzing sensory quality, taste quality and aroma quality. The results showed that compared with Taoyuandaye and Bixiangzao, the fresh leaves of Jianbohuang were more suitable for processing yellow tea, and the yellow tea made from Jianbohuang had golden color in dried tea, delightfully fresh with sweetness, mellow and refreshing in taste, golden with light brightness in the color of tea soup and …
Cold Plasma Technology And Its Effect On The Food Quality And Microorganism, Yang Xinwen, Niu Wenjun, Cheng Junhu, Wang Mansheng
Cold Plasma Technology And Its Effect On The Food Quality And Microorganism, Yang Xinwen, Niu Wenjun, Cheng Junhu, Wang Mansheng
Food and Machinery
This paper briefly described the development status, discharge mode, diagnosis and characterization of active components of cold plasma technology, and discussed the changes of macromolecules such as starch and protein structure in food systems and microbes inactivation in foods, which provides an important theoretical reference and new technical means for the regulation of food quality by cold plasma technology, and also provides theoretical support for the engineering application of this technology.
Research Advances Of Coolants In Tobacco, Wang Ziyan, Han Jingmei, Lei Ping, Mao Deshou, Ren Dabing, Yi Lunzhao, Tang Jianguo
Research Advances Of Coolants In Tobacco, Wang Ziyan, Han Jingmei, Lei Ping, Mao Deshou, Ren Dabing, Yi Lunzhao, Tang Jianguo
Food and Machinery
In this paper, based on the research references on smoke coolants in recent years, based on its classification and synthesis methods, the paper summarizes the common analysis and detection methods of coolants in cigarettes and their effects on the safety of cigarette smoking, and the future smoke.
Research Progress Of Lactic Acid Bacteria Microcapsule Encapsulation Technology And Common Wall Materials, Liu Renjie, Li Zhe, Mao Sining, Liang Shan, Zhao Yue, Wang Yuhua
Research Progress Of Lactic Acid Bacteria Microcapsule Encapsulation Technology And Common Wall Materials, Liu Renjie, Li Zhe, Mao Sining, Liang Shan, Zhao Yue, Wang Yuhua
Food and Machinery
Improving the survival rate of lactic acid bacteria in human gastrointestinal tract, the article outlines the definition and development history of microcapsule encapsulation technology, and the commonly used and new microcapsule encapsulation technology. The advantages and disadvantages of interfacial polymerization, phase separation, spray drying, extrusion and high-pressure electrostatic spray drying methods were analyzed. The method of lactic acid bacteria encapsulation was discussed with the common wall materials of lactic acid microcapsules. Combined with the common wall materials of lactic acid bacteria microcapsules, the lactic acid bacteria encapsulation method was discussed, and the effects of compound encapsulation, multi-layer encapsulation and prebiotic …
Application Of Heat Pipe-Heat Pump And Infrared-Heat Pump In Agricultural Product Processing, Li Xiaoyan, Qiang Qiuqiu, Fan Bowei, Fu Jian, Zhao Yifan, Zhang Lihong
Application Of Heat Pipe-Heat Pump And Infrared-Heat Pump In Agricultural Product Processing, Li Xiaoyan, Qiang Qiuqiu, Fan Bowei, Fu Jian, Zhao Yifan, Zhang Lihong
Food and Machinery
This paper reviews the research progress of heat pipe-heat pump and infrared-heat pump dry agricultural products with the advantages of short time, good quality and energy saving. The drying efficiency and drying quality change in the drying process are analyzed. The problems of heat pipe-heat pump drying technology and infrared-heat pump drying technology are summarized, which provides new ideas for optimizing dry agricultural products.
The Healthy Benefits And Applications Of Red Rice, Wang Li, Zhu Fan, Wang Fawen, Li Yan, Qian Haifeng, Zhang Hui, Qi Xiguang
The Healthy Benefits And Applications Of Red Rice, Wang Li, Zhu Fan, Wang Fawen, Li Yan, Qian Haifeng, Zhang Hui, Qi Xiguang
Food and Machinery
Red rice contains proteins, amino acids, vitamins, trace elements, and some biological active ingredients. Recently, its health benefits had been reported, including lowering blood glucose and total cholesterol level, antioxidant ability, antitumor activity, and so on. This review summarized the nutrients, health benefits and comprehensive utilization of red rice.
Discussion On The Integrated Development Of Chinese Medicine Diet And Foods For Special Dietary Industry, Li Ling, Xia Xinbin, Zhou Liangrong
Discussion On The Integrated Development Of Chinese Medicine Diet And Foods For Special Dietary Industry, Li Ling, Xia Xinbin, Zhou Liangrong
Food and Machinery
Chinese medical diet, like foods for special dietary uses, can provide special or targeted physiological substances and nutrients for specific groups with scientific methods, so as to improve the basic nutrition metabolism of specific groups and reduce unnecessary metabolic burden .This article suggests that the traditional Chinese medicine diet with national characteristics and a long history should be integrated with the special diet food industry, so as to promote the rapid growth of the special diet industry with Chinese characteristics and enhance the international competitiveness of China's special diet food.
Preparation And Identification Of The Monoclonal Antibody Of Kunitz Trypsin Inhibitor From Soybean, Wang Yao, Li Yanhong, Cao Jinbo, Sun Yaning, Xing Yunrui, Chen Xiujin, Deng Ruiguang, Hu Xiaofei
Preparation And Identification Of The Monoclonal Antibody Of Kunitz Trypsin Inhibitor From Soybean, Wang Yao, Li Yanhong, Cao Jinbo, Sun Yaning, Xing Yunrui, Chen Xiujin, Deng Ruiguang, Hu Xiaofei
Food and Machinery
To prepare monoclonal antibodies against soybean Kunitz trypsin inhibitor (KTI) with good immunological characteristics. BALB/c mice were used to immunize with 50 μg of KTI, the antiserum titer and sensitivity were identified via indirect ELISA and indirect competitive ELISA. The mice which serum has better titer and sensitivity were selected for cell fusing, then he hybridoma cell strain which stabilization secreted KTI monoclonal antibody (mAb) were screened. Thereafter, mAb was prepared by inducing ascites in vivo and identified the immunological characteristics. The result indicated that the NO.1 mouse was confirmed to produce the antibody with the highest titre, IC50 …
Simultaneous Determination Of Flavonoids In Fruits Of Rosa Davurica Pall. By Hplc And Measurement Of Their Activities In Vitro, Di Song, Zhong Fangli, Zhang Xiaoli, Wang Xiaolin, Lei Yongping
Simultaneous Determination Of Flavonoids In Fruits Of Rosa Davurica Pall. By Hplc And Measurement Of Their Activities In Vitro, Di Song, Zhong Fangli, Zhang Xiaoli, Wang Xiaolin, Lei Yongping
Food and Machinery
Simultaneous determination of four flavonoids in extracts of the fruits of Rosa davurica pall.(FRDP) by high performance liquid chromatography (HPLC) was carried out, and the in vitro activities of extracts of FRDP, four flavonoids and their compound compatibility were measured by UV method. The results showed that the contents of hyperoside, rutin, quercetin and luteolin of the extract of FRDP were 607.98, 450.63, 187.32 and 12.68 μg/g respectively. The extracts of FRDP, four flavonoids and their compound compatibility all have certain antioxidant activities in vitro, and the activity of extracts of FRDP was stronger than that of compound compatibility. However, …
Effects Of Far-Infrared Radiation Temperature On The Moisture Transfer Of Kiwifruit Slices, Zeng Ya, Liu Yunhong, Zhang Jiayi, Xi Huihan, Hu Runrun
Effects Of Far-Infrared Radiation Temperature On The Moisture Transfer Of Kiwifruit Slices, Zeng Ya, Liu Yunhong, Zhang Jiayi, Xi Huihan, Hu Runrun
Food and Machinery
In order to explore the water diffusion characteristics of kiwifruit during far-infrared radiation drying, low-field nuclear magnetic resonance (LF-NMR) technology was applied to analyze the influence of radiation temperature on internal water states and moisture migration of kiwifruits during drying. The results showed that drying time decreased significantly with the increasing of radiation temperature, and the ratio of average drying rates at radiation temperatures of 120, 160, 200, 240 and 280 ℃ were 1.00∶1.30∶1.54∶2.00∶2.50 respectively. The effective moisture diffusivity values was in the range of 2.85×10-10~7.03×10-10 m2/s, which could be improved with the increase in …