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Articles 421 - 450 of 524
Full-Text Articles in Food Biotechnology
Effects Of Different Storage Temperature On Quality And Fresh-Keeping Of Cynoglossus Semilaevis Gunther, Li Na, Xie Jing
Effects Of Different Storage Temperature On Quality And Fresh-Keeping Of Cynoglossus Semilaevis Gunther, Li Na, Xie Jing
Food and Machinery
The effect of storage temperatures (such as 10, 4, 0 and -3 ℃) on the change of quality characteristics and fresh-keeping of cynoglossus semilaevis gunther was investigated. The changes in TVC, dripping loss, cooking loss, electric conductivity, TVB-N, K value, biogenic amines and total amino acid were studied. Combined with sensory evaluation, it could be concluded that with the extension of storage time, water hold capacity decreased, and electric conductivity, TVB-N, K value, total amino acid, and TVC increased. The change rate of every index decreased by the decreasing of storage temperature. The biogenic amines accumulated during the storage period …
Effects Of Different Frozen Temperatures On The Quality Of Freshwater Prawn (Macrobrachium Rosenbergii), Yu Lixia, Jiang Qixing, Xia Wenshui, Xu Yanshun, Wang Bin, Gao Pei, Yang Fang
Effects Of Different Frozen Temperatures On The Quality Of Freshwater Prawn (Macrobrachium Rosenbergii), Yu Lixia, Jiang Qixing, Xia Wenshui, Xu Yanshun, Wang Bin, Gao Pei, Yang Fang
Food and Machinery
Effects of different frozen temperatures on the quality of freshwater prawn (Macrobrachium rosenbergii) were investigated in this study. The thawing loss, cooking loss, TVB-N, K value, hardness, the shear force and color properties were evaluated. The results showed that after freezing by liquid nitrogen, the water holding capacity and texture properties of freshwater prawn were deteriorated with the extension of storage time. After 20 weeks, the hardness of prawn decreased by 55.79% and 59.45%. The TVB-N value and K value increased with storage time, however, the TVB-N value and K value of prawn frozen at -50 ℃ increased more slowly …
Effects Of The Fresh-Keeping Modes On Quality Of Flowering Chinese Cabbage Under Simulated Transportation, Yang Songxia, Lu Enli, Zhu Lixue, Zhao Junhong
Effects Of The Fresh-Keeping Modes On Quality Of Flowering Chinese Cabbage Under Simulated Transportation, Yang Songxia, Lu Enli, Zhu Lixue, Zhao Junhong
Food and Machinery
In order to obtain the effects of three fresh-keeping modes including the atmosphere controlling fresh-keeping mode, refrigera-ted-humidified fresh-keeping mode and refrigerated fresh-keeping mode on the quality of flowering Chinese cabbage in transportation，the fresh-keeping transportation experiments for flowering Chinese cabbage were carried out applied transportation compartment of multi fresh-keeping modes as experiment platform. The results indicated that, for the transportation within 2 days, the quality of 3 kinds of fresh-keeping modes had no significant difference (P>0.05), and they could maintain flowering Chinese cabbage better quality. For the transportation more than 2 days, controlled atmosphere fresh-keeping mode and refrigerated-humidified fresh-keeping mode …
Effects Of Storage Temperature And Duration On Quality Properties Of Fresh Rice Noodles, Zhu Hong, Wang Fang, Yi Cuiping
Effects Of Storage Temperature And Duration On Quality Properties Of Fresh Rice Noodles, Zhu Hong, Wang Fang, Yi Cuiping
Food and Machinery
Fresh rice noodles at the different temperatures（4, 25, 37 ℃）during 0～30 h storage, the total plate count (TPC), textural properties, cooking properties, color and sensory properties were analyzed. The results indicated that TPC of fresh rice noodles at 4 ℃ were within the safe range during 30 h, the hardness and chewiness of the products were higher than those of the 25 ℃ and 37 ℃ groups at the same storage time, the flexibility was relatively low; at 25 ℃, the TPC of fresh rice noodles were within the safe range during 18 h, the textural properties better than 4 …
Research On Strawberry Automatic Classification Based On The Machine Vision, Yang Tao, Zhang Yunwei, Gou Shuang
Research On Strawberry Automatic Classification Based On The Machine Vision, Yang Tao, Zhang Yunwei, Gou Shuang
Food and Machinery
Aiming at the shortcoming of using a single feature to measure the quality of strawberry in traditional strawberry automatic grading system, we proposed a new method to evaluate the quality of strawberry from the aspects of maturity, mass and shape. Firstly, the H parameters in the HSV color model was calculated and analyzed to determine the maturity of strawberries. Secondly, the mass of strawberry was measured by the image projection area-mass function, and then the K-means clustering method and the discriminant analysis were used to obtain the shape classification of strawberry. Finally, the composite score of strawberry mass and shape …
Isolation And Identification Of Immunoactive Peptides From Rice Protein Hydrolysates With Medium-Pressure Ion Exchange Chromatography, Cheng Yunhui, Mao Tianmi, Huang Lu, Chen Maolong, Wen Li, Xu Zhou
Isolation And Identification Of Immunoactive Peptides From Rice Protein Hydrolysates With Medium-Pressure Ion Exchange Chromatography, Cheng Yunhui, Mao Tianmi, Huang Lu, Chen Maolong, Wen Li, Xu Zhou
Food and Machinery
Medium-pressure ion exchange chromatography was used for further fractionation of rice immunoactive peptides obtained from Trypsin hydrolyzation and gradient ethanol elution with DA201-C macroporous adsorption resin (40% ethanol fraction). The results showed that the optimal parameters for fractionation of RPHs-A3 fractions by WorkBeads 40 Q on a 2.6 cm×40 cm columns were as follows: Sample: 5 mL, 30 mg/mL 40% ethanol fraction; Equilibration buffer A: pH 9.0, 0.01 mol/L Tris-HCl; Elution Buffer B: pH 9.0, 0.01 mol/L Tris-HCl containing 1 mol/L NaCl; Sample flow rate: 1 mL/min; Elution flow rate: 10 mL/min. Five fractions were obtained after NaCl gradient elution. …
Separation And Identification Of The Hypnotic Active Ingredients Of Schisandra Chinensis, Zhang Ziyu, Xu Deping
Separation And Identification Of The Hypnotic Active Ingredients Of Schisandra Chinensis, Zhang Ziyu, Xu Deping
Food and Machinery
To screen the constituents with dormant activity in Schisandra chinensis and analyze the active ingredients. The content of dopamine (DA) in mouse cerebral cortex was detected by high performance liquid chromatography (HPLC), and the sleeping effect was observed by sleep method. The effects of S. chinensis on the hypnotic effect were studied by using macroporous resin column, MCI column and ODS column. Active ingredients with hypnotic effect were separated, and the compounds isolated from active ingredients were structurally identified by nuclear magnetic resonance (NMR). The elution fraction of MCI and the elution fraction of 95% ethanol in macroporous resin significantly …
Comparison On Functional Properties Of Oat Protein Extracted From Different Ways, Xu Yingyi, Xu Yanxia, Wang Yu
Comparison On Functional Properties Of Oat Protein Extracted From Different Ways, Xu Yingyi, Xu Yanxia, Wang Yu
Food and Machinery
The oat flour as raw material, the oat proteins by alkali extraction and acid precipitation (AOP) and enzymatic method (EOP) were comparatively evaluated for functional properties ( solubility, foaming properties, emulsifying properties, amino acid compositions). The results showed that at the isoelectric point of the protein, solubility, foaming and emulsifying properties of AOP and EOP reached minimum, and emulsion stability of AOP and EOP were the highest; with the increase of NaCl concentration, the solubility, foamability, foam stability and emulsifying properties were basically increased first and then decreased, and emulsion stability decreased;The total amino acids of AOP and EOP were …
Optimization Of Extraction Process For Total Triterpenoids In Alisma Orientalis (Sam.) Juzep. By Response Surface Methodology, Shi Feng, Chen Yu, Zhang Yaqin, Ma Liuhui, Dou Mingming, Yang Zhengming, Chen Xingfu
Optimization Of Extraction Process For Total Triterpenoids In Alisma Orientalis (Sam.) Juzep. By Response Surface Methodology, Shi Feng, Chen Yu, Zhang Yaqin, Ma Liuhui, Dou Mingming, Yang Zhengming, Chen Xingfu
Food and Machinery
The optimum conditions of ultrasonic extraction of total triterpenoid from leaves of Alisma orientalis (Sam.) Juzep. were investigated in this study. On the basis of single factor experiments, with yields of total triterpenoid as indexes, the three levels of four factors of Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions by taking extraction temperature ,ethanol concentration, extraction time and the ratio of material to solvent as factors. Results: Optimal extraction technology was as following, i.e. the ethanol concentration of 70%, the extracting for 46.00 min at 57.00 ℃, and the ratio of material to solvent …
Isolation And Purification Of Polysaccharides Fromforage Mulberry (Morus Alba L.) And The Research Of Their Biological Activity, Wang Lingjia, Shu Xiaomeng, Xin Wen, Zhang Chisong, Chen Xianggui, Yang Xiao
Isolation And Purification Of Polysaccharides Fromforage Mulberry (Morus Alba L.) And The Research Of Their Biological Activity, Wang Lingjia, Shu Xiaomeng, Xin Wen, Zhang Chisong, Chen Xianggui, Yang Xiao
Food and Machinery
Purpose Optimization of enzymatic extraction process for total polysaccharides from mulberry (Morus alba L.) leaves and comparison of biological activity before and after purification. Methods The polysaccharide from mulberry leaves was extracted by cellulase and the optimal extraction parameters were optimized by single factor and orthogonal experiment. The primary purification was carried out by Sephadex G-100. The antioxidant activity and α-glucosidase inhibitory activity of polysaccharides before and after purification were compared. Result The optimal process parameters was pH 6, enzyme concentration 1.0%, extraction temperature 50 ℃, time 50 min; total polysaccharides after purification had higher total antioxidant capacity and DPPH, …
Process Optimization For Purification And Preparation Of Hairy Antler Grounds Antioxidant Peptide Assisted By High-Intensity Pulsed Electric Fields, Jiang Wei, Jin Shenglang, Yin Yongguang
Process Optimization For Purification And Preparation Of Hairy Antler Grounds Antioxidant Peptide Assisted By High-Intensity Pulsed Electric Fields, Jiang Wei, Jin Shenglang, Yin Yongguang
Food and Machinery
Antioxidant peptides were prepared from hairy antler grounds by high-intensity pulsed electric fields (PEF) jointed bromelain, and then they were isolated and purified. The degree of hydrolysis and scavenging rates of DPPH· was considered as test indexes. According to the results of the single factor experiment, the response surface methodology (RSM) experiment was conducted on three factors at five levels to analyze the factors and interaction effects on the scavenging rates of DPPH·. Then the macroporous resin was used to desalt, separate and purify hairy antler antioxidant peptides, and the antioxidant activities of hairy antler peptides were also analyzed. The …
Purification Of Lignans From Camphor Tree Leaves By Macroporous Adsorption Resins, Wu Pingxiang, Li Zhonghai
Purification Of Lignans From Camphor Tree Leaves By Macroporous Adsorption Resins, Wu Pingxiang, Li Zhonghai
Food and Machinery
In this study, six different macroporous adsorption resins were evaluated for their use to purify lignans ethanol-extraction of camphor tree leaves，a suitable macroporous adsorption resin was selected as the purification material. The effects were investigated, such as sample concentration, flow rate, volume of sample on adsorption rate of macroporous resin, and the effects of concentration, flow rate and dosage of eluent on desorption rate of the macroporous resin were also studied . The optimal purification process of lignan with the macroporous resin was studied by the orthogonal experiment. The purication parameters optimized by adsorption and desorption were determined as follows: …
Research Progress On Extraction Technology And Functional Activity Of Purple Sweet Potato Pigment, Zhao Erlao, Yan Wei, Gao Ziyi, Huangfu Yangxin, Zhao Sanhu
Research Progress On Extraction Technology And Functional Activity Of Purple Sweet Potato Pigment, Zhao Erlao, Yan Wei, Gao Ziyi, Huangfu Yangxin, Zhao Sanhu
Food and Machinery
Based on the literature learning method and summariz-ation, the domestic research progresses on the extraction technology and the functional activity of purple sweet potato pigment were reviewe. The application prospect of the purple sweet potato pigment had also been presented in this paper, which provided a reference for its further research and development.
Research Progress On Effects Of The Ozone Treatment On Post-Harvest Physiology And Storage Quality Of Fruits, Jiang Bao
Food and Machinery
As a non-thermal technology, ozone is being applied to food and fruit which become more and more popular. On the basis of the analysis of the influencing factors for preservation effect of ozone, the latest process in effects of ozone on post-harvest physiology and storage quality of fruits were reviewed, providing a basis and reference for the ozone better applied to fruit storage and preservation.
Effects Of Rice Bran Oil On Quality Of The Spread Processed Cheese, Xu Hangrong, Zheng Yuanrong, Liu Zhenmin
Effects Of Rice Bran Oil On Quality Of The Spread Processed Cheese, Xu Hangrong, Zheng Yuanrong, Liu Zhenmin
Food and Machinery
To provide a theoretical basis for the research and development of high-quality processed cheese with rice bran oil, the effects of different levels of rice bran oil instead of butter on the quality of the spread processed cheese were studied. The determination of the pH, firmness, stickness and sensory evaluation of the processed cheese with various substitution ratios of rice bran oil were compared and analyzed. The results showed that the pH values of the samples were in the normal pH range, and the firmness and stickiness of samples decreased with the increase of the amount of rice bran oil. …
Optimization Of Alcohol Fermentation And Acid Characteristics Analysis In Loquat Vinegar Production, Duan Zhenzhen, Zhou Caiqiong, Yuan Min, Chang Rong
Optimization Of Alcohol Fermentation And Acid Characteristics Analysis In Loquat Vinegar Production, Duan Zhenzhen, Zhou Caiqiong, Yuan Min, Chang Rong
Food and Machinery
Red flesh loquat named Jinhua No.1 was used as experimental material to optimize alcohol fermentation process and analyze the acidity characteristics of loquat vinegar in this study．The effects of yeast species and inoculation amount, pH, fermentation temperature and initial sugar content on the quality of loquat vinegar were studied, and the optimal fermentation conditions were determined by the alcoholic degree as the response value. The results showed that the yeast strain Yb was the dominant yeast strain of loquat vinegar, the optimal process parameters were that the yeast inoculation amount 0.05% was fermented at 25 ℃ and pH 3.5. Under …
Optimization Of The Compound Fermentation Process Of Red Chili And Pickled Chili Juice, Wang Wancheng, Zhao Lingyan, Deng Fangming
Optimization Of The Compound Fermentation Process Of Red Chili And Pickled Chili Juice, Wang Wancheng, Zhao Lingyan, Deng Fangming
Food and Machinery
Red line chili and pickled chili juice were mixed in a 11 (w/w) ratio, and inoculated with Lactobacillus plantarum, Lactobacillus acidophilus and Leu-conostoc mesenteroides to get fermentation. The lactobacillus inoculation quantity, fermentation temperature and fermentation time were selected as the experimental factors, and the lactic acid content was used as the response value. The single factor and response surface test were designed to optimize the fermentation process. The best conditions for optimization were as follows: inoculation 6%, fermenting at 35 ℃ for 6 days. The validation test results showed that the fermented pepper products’ lactic acid content was 0.74% and …
Optimization Of The Quantitative Determination Method Of The Burning Cigarette Ash Integration Based On Imagej, Feng Yajie, Wang Jianmin, Liang Miao, Mu Lin, Ma Shengnan, Wang Huaixu, Xu Linghe
Optimization Of The Quantitative Determination Method Of The Burning Cigarette Ash Integration Based On Imagej, Feng Yajie, Wang Jianmin, Liang Miao, Mu Lin, Ma Shengnan, Wang Huaixu, Xu Linghe
Food and Machinery
In order to improve the accuracy and repeatability of the integration quantitative determination method based on ImageJ for the ash from the burning cigarette., an optimized scheme was conducted in this study. The scheme was optimized from three aspects, including the selection of cigarette combustion surface, the selected height as well as the width of the burning cigarette columnar ash. The established optimal scheme was as follows: ① Taking pictures of the burning cigarette when it was burned to 1 cm away from the tipping paper. ② Using a height of 25 mm, 6.46 mm width rectangular box to select …
The Differences Of Taste Characteristics Of Commercial Natural Mature Cheeses, Wang Lei
The Differences Of Taste Characteristics Of Commercial Natural Mature Cheeses, Wang Lei
Food and Machinery
The main taste components of eight typical natural mature cheeses were analyzed by gas chromatography-mass spectrometry and amino acid analyzer. The taste activity values and sensory evaluation were used to clarify the differences among the eight kinds of cheeses, so as to provide a theoretical basis for precise control of cheese flavor and market positioning. The results showed that in addition to Goat Cheese and Brie Cheese, free amino acids contributed to the taste of the other six type of cheeses. The common taste amino acids were valine and lysine. Cheeses were bitter and sweet. And blue Cheese had the …
Effects Of Oxygen Vectors On Microbial Production Of Avilamycin, Zhang Mei, Song Yuanda, Liu Qing, Wang Youling, Wu Han
Effects Of Oxygen Vectors On Microbial Production Of Avilamycin, Zhang Mei, Song Yuanda, Liu Qing, Wang Youling, Wu Han
Food and Machinery
In order to improve oxygen delivery efficiency and dissolved oxygen levels for the production of avilamycin in the fermentation broth, the effects were studied, including the various oxygen carriers on the synthesis of avilamycin and the growth of Streptomyces viridochromogenes. The results showed that Tween-80 was the most effective vector to promote growth and the ability of Streptomyces viridochromogene to produce avilamycin.With the addition of 3 g/L tween at 8 h fermentation ,the biomass reached 11.82 g/L and the avilamycin titer increased to 340 mg/L, which was 20.63% and 21.42% higher than the control group. Result show that the oxygen …
Study On Extraction Processof Total Flavonoids In Dandelion By Using The Compound Enzymes And Their Antioxidant Activities, Zhang Zhi, Liu Yang, Yin Wenzhe, Hua Hongling, Yu Henglin, Zhang Zhijun
Study On Extraction Processof Total Flavonoids In Dandelion By Using The Compound Enzymes And Their Antioxidant Activities, Zhang Zhi, Liu Yang, Yin Wenzhe, Hua Hongling, Yu Henglin, Zhang Zhijun
Food and Machinery
The effects on the yield of total flavonoids were studied by reference enzyme dosage, pH value, ratio of material to liquid, ethanol concentration, enzymolysis temperature and time. A 4 factor with 3 horizontal response surface test optimization were conducted. The optimal extraction conditions of the total flavonoids of dandelion were as follows: 122 (g/mL), pH 4.5, 52% ethanol concentr-ation, the hydrolysis temperature was 51 ℃. Under these condi-tions, the yield of total flavonoids was 15.09 mg/g. The results of in vitro functional experiments showed that the total flavonoids extract of dandelion had strong antioxidant activity and scavenging ability against free …
Study On Isolation, Structure Characterization And Radical Scavenging Activity Of Cyanobacteria Polysaccharide, Huang Yijia, Wu Jianrong, Zhu Li, Zhan Xiaobei
Study On Isolation, Structure Characterization And Radical Scavenging Activity Of Cyanobacteria Polysaccharide, Huang Yijia, Wu Jianrong, Zhu Li, Zhan Xiaobei
Food and Machinery
An effective method to extract Cyanobacteria polysaccha-ride was established in this study and a high yield of polysaccharide (25.1%) was achieved by optimizing extraction condition (hot water with ultrasonic treatment). After purification, a main purified polysaccharide (CB-2-1) with molecular weight of 8.4 kDa was collected and the compositions included rhamnose, arabinose, xylose, mannose, glucose and galactose, with mole percentages of 0.51%, 13.35%, 18.21%, 51.38%, 4.87% and 11.67%, respectively. The results of NMR showed that the CB-2-1 component of cyanobacteria polysaccharide was mainly α-type glycosidic bond. Moreover, the purified CB-2-1 polysaccharide had great anti-oxidant activities. The highest scavenging activities of DPPH …
Preparation Of Low Lactose Milk With High Hydrolysis Rate And Analysis Of Sugar, Wu Taoying, Cheng Yunhui, Zhao Liangzhong, Ouyang Lin, Xu Zhou
Preparation Of Low Lactose Milk With High Hydrolysis Rate And Analysis Of Sugar, Wu Taoying, Cheng Yunhui, Zhao Liangzhong, Ouyang Lin, Xu Zhou
Food and Machinery
To determine the optimum conditions for the production of low lactose milk by enzyme method, the hydrolysis process of low lactose milk was optimized by the response surface method, with the hydrolysis rate of lactose as an index. The results were as follows: the ratio of enzyme to substrate (\[E\]/\[S\]) was 1.50‰, and the hydrolysis was undertaken at 38.15 ℃ for 3.0 h. Under these conditions, the lactose hydrolysis rate of low lactose milk was 92.67%. Moreover,14.02% of the oligosaccharides were detected by HPLC detection, which showed β-galactosidase could hydrolysis lactose and turn glycosidic.
Study On The Transferring Of The Gene Fragment From The Genetically Modified Cottonseed Hulls To The Edible Fungi, Bai Yalong, Suo Yujuan, Lin Ting, Shen Yuanyuan, Zhao Xiaoyan, Liu Haiyan, Zhou Changyan
Study On The Transferring Of The Gene Fragment From The Genetically Modified Cottonseed Hulls To The Edible Fungi, Bai Yalong, Suo Yujuan, Lin Ting, Shen Yuanyuan, Zhao Xiaoyan, Liu Haiyan, Zhou Changyan
Food and Machinery
It is a question in the field of food-safety that whether foreign genetically modified genes would transfer from the genetically modified cottonseed hulls to edible fungi. In this study, 15 Tremella fuciformis samples and 10 Pleurotus ostreatus samples (the cultivation media contained 75% and 92% genetically modified cottonseed hulls, respectively) collected from cultivation meida were evaluated by PCR to confirm whether the foreign genetically modified genes, CaMV 35S, NOS, CpTⅠ, Cry IA(c), were present. In addition, 47 samples which were probably grown in the media containing cottonseed hulls, were collected from the large and medium-sized supermarkets and farm produce markets. …
Characteristic Difference Of Porcine Tissues By Terahertz Attenuated Total Reflectance Spectroscopy, Qi Liang, Zhao Maocheng, Zhao Jie, Tang Yuweiyi
Characteristic Difference Of Porcine Tissues By Terahertz Attenuated Total Reflectance Spectroscopy, Qi Liang, Zhao Maocheng, Zhao Jie, Tang Yuweiyi
Food and Machinery
To investigate the feasibility of testing pork quality by terahertz (THz) spectroscopy nondestructively, refractive indexes and extinction coefficients of fat, muscle and skin of porcine tissue were collected by attenuated total reflection (ATR) mode in the range of 0.2～2.0 THz. The results showed that THz spectra of these tissues were different obviously in 0.2～1.6 THz. Water content was found to be a main factor affecting THz absorption of fresh tissues. The optical characteristic difference in THz band of different tissues could be utilized to identify tissue types rapidly and nondestructively. Moreover, it could be predicted that the pork quality could …
Antioxidant And Uv-Induced Fibroblasts Photoaging Repair Effects Of Polyphenol From Cortex Mori, Wu Yongxiang, Wu Liping, Wang Weidong, Kim Taewan
Antioxidant And Uv-Induced Fibroblasts Photoaging Repair Effects Of Polyphenol From Cortex Mori, Wu Yongxiang, Wu Liping, Wang Weidong, Kim Taewan
Food and Machinery
The antioxidant activity of polyphenol from Cortex Mori (CMP) was examined by 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays. Fibroblasts HS68 cells were induced by ultraviolet (UV) irradiation to establish the photoaging model. Cell proliferation was investigated using a methyl thiazolyl tetrazolium (MTT) assay. The content of procollagen typeⅠprotein (PIP) in cells was measured by enzyme-linked immunosorbent assay (ELISA). The matrix metalloproteinase-1 (MMP-1) and PIP mRNA expressi-ons were detected by qRT-PCR. CMP exhibited significant antioxi-dant activity in ABTS, DPPH free radical scavenging assays, with IC50 values of 1.49, 8.94 μg/mL, respectively. The IC50 values of butyl …
Plastein Reaction To Modify The Ace Inhibitory Peptides Of Potato Protein, Gao Dandan, Ma Zhongren, Saimaiti Reziwanguli, Tian Xiaojing, Li Mingsheng, Chen Shien
Plastein Reaction To Modify The Ace Inhibitory Peptides Of Potato Protein, Gao Dandan, Ma Zhongren, Saimaiti Reziwanguli, Tian Xiaojing, Li Mingsheng, Chen Shien
Food and Machinery
Potato protein was hydrolyzed by trypsin to prepare for ACE inhibitory peptides in our previous researches (the ACE inhibition rates was 61.20%±0.15%), and we used plastein reaction to modify potato protein hydrolysates for enhancing the ACE inhibitory activity of peptides in this study. The influences of the kinds of added amino acids, the substrate/enzyme ratio, pH value, the temperature and hydrolysis time on ACE inhibitory activity of the plastein reaction modification of potato protein hydrolysates were investigated. To achieve the maximum ACE inhibitory activity in the plastein reaction modification of cottonseed protein hydrolysates, the response surface methodology was applied to …
Enhancement Of Docosahexaenoic Acid Production In Schizochytrium Sp. 31 Combining Ethylmethylsulfone And Atmospheric And Room Temperature Plasma Treatment, Zhao Ben, Wang Wu, Li Changling, Yang Hailin
Enhancement Of Docosahexaenoic Acid Production In Schizochytrium Sp. 31 Combining Ethylmethylsulfone And Atmospheric And Room Temperature Plasma Treatment, Zhao Ben, Wang Wu, Li Changling, Yang Hailin
Food and Machinery
For establishing an efficient and stable mutagenesis method on breeding Schizochytrium DHA higher producers, the wild-type strain named Schizochytrium sp. 31 was mutated combining Ethylmethylsulfone (EMS) and Atmospheric and Room Temperature Plasma (ARTP) treatment. Mutagenic efficiency, fermentation indexes of the mutants were compared and analyzed. Under this compound mutagenesis method, the positive mutation rate reached 32.2%, which was much higher than that under other two single-factor mutagenesis methods. Besides, DHA production and DHA percentage in total fatty acids reached to 7.2 g/L and 43.2%, which were 35.6% and 19.2% higher than that of the wild-type strain, respectively. After five rounds …
Study On Technology And Enzymatic Property Of Xylanase Produced From Engineered Escherichia Coli, Liu Guofeng
Study On Technology And Enzymatic Property Of Xylanase Produced From Engineered Escherichia Coli, Liu Guofeng
Food and Machinery
In this study, the extraction technology and enzymatic property of xylanase produced from engineered Escherichia coli are fully examined. The extraction of production process of xylanase was determined and the forage enteric xylanase was successfully obtained after cell broken (high-speed ball grinding), solid-liquid separation, aseptic (filtering) hollow fiber membrane, ultrafiltration concentra-tion and drying packaging orderly. This production showed desirable thermal stability and its release rate was 5% and 98.5% in pH 2.5 and pH 5.5 phosphate buffer, respectively. The product appearance exhibited good gloss and it could meet the requirements of feed enzyme market well. The xylanase showed the maximal …
Study On Compatibility Of Tallow And Four Kinds Of Plant Oil, Yao Di, He Xinyi, Yan Xichun, Li Xu, Zhen Runying
Study On Compatibility Of Tallow And Four Kinds Of Plant Oil, Yao Di, He Xinyi, Yan Xichun, Li Xu, Zhen Runying
Food and Machinery
To enrich the variety of tallow products and reduce its melting point and hardness, the melting point and fatty acid composition properties of tallow, rapeseed oil, soybean oil, olive oil and cooking oil were analyzed. With the binary compatibilities of tallow and other four kinds of plant oil, their compatibility was studied through isothermal curve (SFC-T) and deviation curve (△SFC-T). The results suggested that four kinds of binary compatibilities mainly showed partial crystal phenomenon at different temperatures. The linear relationship of isothermal curve was good at 0～40 ℃ centigrade of each group(R2＞0.95). Among the four plant oils, rapeseed …