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Articles 121 - 150 of 524
Full-Text Articles in Food Biotechnology
Optimization Of The Extraction Of Truffle Polysaccharide By Multi-Enzyme Hydrolysis, Qing Yuan, Chen Tiantian
Optimization Of The Extraction Of Truffle Polysaccharide By Multi-Enzyme Hydrolysis, Qing Yuan, Chen Tiantian
Food and Machinery
The polysaccharide was extracted by compound enzyme method from the Indian truffle. Through single factor and uniform design experiments, the effect on the yield of truffle polysaccharide were investigated, including the enzyme ratio, zymolytic time and temperature, enzyme dosage, material/ liquid ratio and pH value. According to U10 (108) experiment, the effects of six single factors as well as their interactions on the yield of polysaccharide were studied. The optimal process parameters were predicted and verified. The results showed that the best extraction conditions of truffle polysaccharide was as follows: dispase and cellulase ratio of 1∶7 （g/g）, …
High-Throughput Screening And Study Of Cftr Specific Inhibitor-Cftrinh-172, Luan Jian
High-Throughput Screening And Study Of Cftr Specific Inhibitor-Cftrinh-172, Luan Jian
Food and Machinery
The small-molecule specific inhibitor, CFTRinh-172, is the standard reference for the researches. The progresses of CFTRinh-172 in chemical structure, mechanisms of action, and pharmacokinetics were summarizes in this review. CFTRinh-172 plays an important role in the treatment and drug development of malignant diseases such as cholera, polycystic kidney disease and leukemia. Moreover, CFTRinh-172 is the specific molecular inhibitor of the CFTR chloride channel. However, the side-effects and the poor water solubility restrict its application.
Research Progress Of The Effects Of Protein Modification On The Processing Properties Of Poultry Egg Powder, He Weiming, Ye Jinsong, Shi Jie
Research Progress Of The Effects Of Protein Modification On The Processing Properties Of Poultry Egg Powder, He Weiming, Ye Jinsong, Shi Jie
Food and Machinery
In this review, the effects of egg powder were summarized from the aspects of physical method, chemical method and enzymatic modification, and the traits of these methods were analyzed and compared. Moreover, the future development of egg powder was prospected, so as to enhance the processing property of egg powder and expand the application range.
Research Progress On Bacteriostatic Mechanism Of Plant Derived Bio-Preservatives Against The Microbodies In The Aquatic Products, Lan Weiqing, Chen Mengling, Wang Meng, Xie Jing
Research Progress On Bacteriostatic Mechanism Of Plant Derived Bio-Preservatives Against The Microbodies In The Aquatic Products, Lan Weiqing, Chen Mengling, Wang Meng, Xie Jing
Food and Machinery
Based on the advantages and disadvantages comparison in different sources of bio-preservatives, the classification of plant source bio-preservatives was described, and their bacteriostatic mechanism in aquatic products was introduced. Moreover, its existing problems and improvement suggestions were put forward, and the future development of plant derived bio-preservatives on the preservation of the aquatic products was also prospected.
Research Progress On Nutrients And Processing Technology Of Peony Seed Oil, Fan Yongkang, Xiang Ting, Wang Wei, Shen Jianfu
Research Progress On Nutrients And Processing Technology Of Peony Seed Oil, Fan Yongkang, Xiang Ting, Wang Wei, Shen Jianfu
Food and Machinery
In this paper, the nutritional components of peony seed oil, including fatty acid, unsaponifiable compounds and trace elements, were summarized, and the functional properties, extraction process, refining process and processing of product oil of peony seed oil were also summarized and analyzed in order to provide reference for the further development of peony seed oil.
Progress In The Processing Technology Of Silkworm Pupal Oil, Shi Wei, Liao Sentai, Zou Yuxiao, Wang Weifei, Li Erna
Progress In The Processing Technology Of Silkworm Pupal Oil, Shi Wei, Liao Sentai, Zou Yuxiao, Wang Weifei, Li Erna
Food and Machinery
Summarizes the research progress in the extraction, refining, and anti-oxidation of silkworm pupal oil in China and abroad in recent years. Regarding with the technical problems that restrict the development of industry, such as deacidification，deodorization and so on, this paper presents the key research directions in the future in this field. Based on the existing research, analyze the lipid composition characteristics of silkworm pupal oil, develop special extraction and refining technology for silkworm pupal oil in the future, especially enzymatic deacidification and low-temperature deodoriza-tion technology; study the anti-oxidation technology which is suitable for silkworm pupal oil, develop a silkworm pupal …
Optimization On Process Conditions For Acetic Acid Bacteria Bran-Koji, Pan Wanshu, Peng Yang, Du Dazhao, Wu Yuanming, Liu Fang, Wang Rui, Ao Xiaolin, Liu Shuliang
Optimization On Process Conditions For Acetic Acid Bacteria Bran-Koji, Pan Wanshu, Peng Yang, Du Dazhao, Wu Yuanming, Liu Fang, Wang Rui, Ao Xiaolin, Liu Shuliang
Food and Machinery
Acetobacter pasteurianum C9-4 was used as bran fermentation strain, and the preparation conditions of acetic acid bacteria bran koji fermentation were optimized by response surface method. The result showed that the best preparation conditions were: content of acetic acid 1.03 mL/100 g, condent of ethanol 2.09 mL/100 g and content of inoculation 4.09 mL/100 g. Under the condition, the production reached 1.38×108 CFU/g. The culture was expanded in vinegar factory with the optimized parameter, the total number of acetic acid bacteria could reach 108 CFU/g in the process of expanding the culture. This experiment provides a reference for …
Study On Elastic Modulus Of Fiber Layer Of Dried Betel Nut By Three Points Bend Method Determination, Kang Xiaoning, Li Liang, Ji Jianbang
Study On Elastic Modulus Of Fiber Layer Of Dried Betel Nut By Three Points Bend Method Determination, Kang Xiaoning, Li Liang, Ji Jianbang
Food and Machinery
A new method for evaluating the mechanical properties of areca nut fiber layer is established. The elastic modulus of areca nut was determined by three-point bending method and the parameters were optimized. Scanning electron microscope (SEM) and sensory evaluation test were used to verify the scientific nature of the method. The results showed that the moisture content and width of the sample had a certain effect on the elastic modulus, but the measuring conditions had no significant effect on the elastic modulus, and the elastic modulus of areca nut dried fruit could be objectively and scientifically measured by the three-point …
Screening And Expression Identification Of Staphylococcus Aureus-Inhibiting Peptide Genes From Lactic Acid Bacteria, Ren Dayong, Zhu Jianwei, Liu Hongyan, Yu Hansong
Screening And Expression Identification Of Staphylococcus Aureus-Inhibiting Peptide Genes From Lactic Acid Bacteria, Ren Dayong, Zhu Jianwei, Liu Hongyan, Yu Hansong
Food and Machinery
The 7 strains with antimicrobial activity were screened as samples and verification by PCR amplification has antimicrobial peptide genes of PlnF, PlnE, PlnN, PlnJ, and PlnK. Then designed a primer to introduce Nco I and Xho I sites into both ends of the antibacterial peptide genes with the treated of pET28a plasmid to obtain recombinant plasmid using T4 ligase, and the pET28a-peptide was transformed into E.coli BL21(DE3). The experiment results showed that the inhibition zone of the pET28a-peptide was the PlnF, after 0.5 mmol/L IPTG inducted for 6 h after the OD600=0.6 of the E.coli LB. The cell …
Study On The Drying Kinetics Of Longan With Intermittent Vacuum-Microwave, An Kejing, Xiao Gengsheng, Wei Lai, Yu Yuanshan, Tang Daobang, Wen Jing, Lin Xian
Study On The Drying Kinetics Of Longan With Intermittent Vacuum-Microwave, An Kejing, Xiao Gengsheng, Wei Lai, Yu Yuanshan, Tang Daobang, Wen Jing, Lin Xian
Food and Machinery
Aiming at the problem of uneven heating of longan, excessive microwave drying rate and local over-focus, the vacuum microwave drying was carried out by the combination of intermittent microwave and variable power microwave. In this paper,the changes of water ratio and drying rate in the vacuum microwave drying process were analyzed, such as the power density, vacuum degree and loading quantity. Seven thin layer fitting models were tested. The orthogonal experiment of three factors and three levels for color, total phenolic content and rehydration evaluation was established. The optimum intermittent vacuum microwave drying process of longan was optimized. The results …
Cfd Simulation Analysis And Experimental Verification Of Twin Screw Compressor, Long Ji, He Xueming, Jiang Zhengang
Cfd Simulation Analysis And Experimental Verification Of Twin Screw Compressor, Long Ji, He Xueming, Jiang Zhengang
Food and Machinery
In view of the complex and time changing characteristics of the fluid domain in the twin screw compressor, and in order to study it’s performance characteristics in detail, used STAR CCM＋software to do the computational fluid dynamics (CFD) analysis, and the experimental platform was built to verify the accuracy of the simulation results. By comparing the pressure values of the simulated monitoring points and the experimental measured points corresponding to them, both of them have good consistency in the pressure value and the period of pressure fluctuation.
Improvement Of Transmission Shaft Connecting Ch And Cv In Gdx2 Packer, Wang Guangsheng, Wang Wenhui, Cao Xingqiang, Wang Zhongqing, Yang Guanglu
Improvement Of Transmission Shaft Connecting Ch And Cv In Gdx2 Packer, Wang Guangsheng, Wang Wenhui, Cao Xingqiang, Wang Zhongqing, Yang Guanglu
Food and Machinery
The transmission shaft connecting CH and CV in GDX2 packer always breaks down due to attrition. Its integrated design and small repair space make it time-and-energy consuming to replace the damaged transmission shaft, which inspires us to improve itsuniversal joints. Based on its original transmission principle, we substitute the fast respectively demountable units for the transmission gear, which made the installment more efficient. While repairing, only the broken units needed to be beplaced. The application result showed that the improved universal joints function reliably, with less labors, and greatly increased the effective operation rate because of its simple structure.
Design Of A Domestic Small Solar Composting Box, Yu Peibin, Chen Jianxin, Li Yanwu
Design Of A Domestic Small Solar Composting Box, Yu Peibin, Chen Jianxin, Li Yanwu
Food and Machinery
In view of the current market common kitchen waste composting box is not easy to keep warm, the composting time is long, the effect of drainage is poor, the odor is heavy and so on. A small household composting box is designed and its spatial structure is designed and optimized. The device includes several modules, such as solar collector, solar panel, ventilator, feeding port, leachate screen, tail gas treatment and so on. It can better meet the requirements of high temperature aerobic composting process. It uses solar energy for the whole system of heat preservation, ventilation and energy supply, reducing …
Optimization Of Polysaccharides Extractedand Analysis From Zingiber Officinale Rosc, Liao Dengwei, Huang Dechun, Cheng Shujie, Cao Chongjiang, Chen Guitang
Optimization Of Polysaccharides Extractedand Analysis From Zingiber Officinale Rosc, Liao Dengwei, Huang Dechun, Cheng Shujie, Cao Chongjiang, Chen Guitang
Food and Machinery
The polysaccharides from Zingiber officinale Rosc were extracted by hot water extraction method, ultrasonic ice bath extraction method and complex enzymatic hydrolysis method. The effects of different methods on the extraction were investigated by the UV spectra image and the scanning electron microscope image of ginger residue. The results showed that the polysaccharides extraction rates of the three different extraction processes under optimal extraction conditions were (11.74±0.23)%, (7.00±0.04)%, and (20.93±0.20)%, and the yields were (6.53±0.08)%, (2.56±0.11)%, and (14.67±0.32)%, the purity was (86.53±0.20)%, (59.84±0.06)%, and (73.59±0.19)%. Comparing with three different methods, the enzymatic hydrolysis method had the highest extraction rate and …
Ultrasonic-Assisted Extraction Essential Oil Of Lemon And Chemical Composition Analysis, Xiao Juan, Zhou Kang, Hu Bin, Su Zhao
Ultrasonic-Assisted Extraction Essential Oil Of Lemon And Chemical Composition Analysis, Xiao Juan, Zhou Kang, Hu Bin, Su Zhao
Food and Machinery
Ultrasonic-assisted hydrodistillation extraction (UAHE) essential oil from lemon peel was investigated. On the basis of single-factor test, response surface methodology was applied to optimize the parameters of extraction, including ultrasonic power, solvent to material ratio, ultrasonic time, and distilling time on the yield of essential oil. Meanwhile, the volatile composition was analyzed by GC-MS. In addition, the microscopic structures of material before and after various extraction methods are observed to clarify extraction mechanisms. The optimal extraction conditions were ultrasonic power of 320 W, solvent to material ratio of 15.5∶1 (mL/g), ultrasonic time of 36 min, distillation time of 140 min. …
Determinants Factors And The Strategies Of Improving Thermostability Of Thermophilic Α-Amylase, Li Caiming, Chen Shuangdi, Gu Zhengbiao, Hong Yan, Cheng Li, Li Zhaofeng
Determinants Factors And The Strategies Of Improving Thermostability Of Thermophilic Α-Amylase, Li Caiming, Chen Shuangdi, Gu Zhengbiao, Hong Yan, Cheng Li, Li Zhaofeng
Food and Machinery
This review clarified the microbial origin and properties of thermophilic α-amylase, and analyzed eight factors affecting their thermostability. The strategies to improve the thermostability of thermophilic α-amylase were proposed, which provided a reference for improving the thermostability technology of thermophilic α-amylase.
Effect Of Enzymatic Glycosylation Modification On Partial Structural And Rheological Properties Of Zein, Qu Yue, Wang Xiaojie, Liu Xiaolan, Cong Wansuo
Effect Of Enzymatic Glycosylation Modification On Partial Structural And Rheological Properties Of Zein, Qu Yue, Wang Xiaojie, Liu Xiaolan, Cong Wansuo
Food and Machinery
In order to improve the bioavailability of zein, which was modified by using the microbial transglutaminase and chitosan. Effects of the enzymatic glycosylation modification on the partial structural and rheological properties of zein were studied. Compared to original zein, the free sulfhydryl groups of the glycosylated zein decreased by 32.37 μmoL/g, the denaturation temperature and the denaturation enthalpy of the glycosylated zein increased by 11 ℃ and 72.3 J/g, respectively. These results suggest that thermal stability of the zein was increased by enzymatic glycosylation reaction.At shearing rate ranging from 1 to 100 s-1, the dispersions of zein and …
Extraction Of Soybean Oil Body Based On Soymilk Model System, Tian Qiying, Hua Yufei, Chen Yeming, Kong Xiangzhen, Zhang Caimeng, Wang Jing
Extraction Of Soybean Oil Body Based On Soymilk Model System, Tian Qiying, Hua Yufei, Chen Yeming, Kong Xiangzhen, Zhang Caimeng, Wang Jing
Food and Machinery
In order to study the volatile flavor of soymilk, a soymilk simulation system with soybean oil body as substrate was constructed, The effects of different pH on the components, particle size and flavor of soybean oil body extracted from soybean milk were studied in the oil body extraction process. The results showed that when the extraction pH increased from 7 to 11, the content of oil body protein decreased by 38.40%, the neutral fat content increased by 7.26%, and the phospholipid content decreased by 49.31%. The oil particle size decreased gradually by 89.2 nm and tended to be stable. Volatile …
Study On Replacement Of Nitrite By Peony Extract And Lactic Acid Bacteria In Low-Temperature Emulsion Sausage, Yuan Ling, Zhuge Bin, Yu Sida, Zong Hong, Lu Xinyao
Study On Replacement Of Nitrite By Peony Extract And Lactic Acid Bacteria In Low-Temperature Emulsion Sausage, Yuan Ling, Zhuge Bin, Yu Sida, Zong Hong, Lu Xinyao
Food and Machinery
The effects of peony extracts and lactobacillus fermentation broth as nitrite substitute were investigated on color, texture characteristics, lipid-oxidation, microbiological properties and flavor of low-temperature emulsion sausages. The results showed that 0.3% peony extracts and 3% lactobacillus fermentation broth could replace two-thirds of dosage of sodium nitrite in low-temperature emulsion sausages, and its redness value (a*) and the degree of fat oxidation were similar to the effect of sausage with 150 mg/kg sodium nitrite, which could maintain good texture characteristics of the sausage. In addition, the total bacteria number of sausage containing peony extract and lactobacillus fermentation broth …
Study On Edible Quality And Protein Stability Of Beef And Goat In Shaanxi Province, Wei Yanchao, Cao Jianxin, Liu Manshun, Liu Yongfeng
Study On Edible Quality And Protein Stability Of Beef And Goat In Shaanxi Province, Wei Yanchao, Cao Jianxin, Liu Manshun, Liu Yongfeng
Food and Machinery
The differences were studied including color, tenderness, pH, protein degradation, and other indicators of beef and mutton at the best slaughter ages of cattle and sheep and the high and low-grade parts of the meat. Samples of longissimus dorsi (YH, NH) and abdominal muscles (YL, NL) of post-mortem Qinchuan beef and horizontal goat meat were taken for determination of pH, color, shear, sarcoplasmic and myofibrillar proteins, the solubility and degradation polymerization conditions. The results shown that the pH value of YL was significantly higher than that of YH (P＜0.05), there was no significant difference between the two parts of beef …
Drying Characteristics Of Direct-Contact Ultrasound Enhanced Hot-Air Drying Of Pear Slices, Sun Changying, Liu Yunhong, Zeng Ya, Shi Xiaowei, Xi Huihan
Drying Characteristics Of Direct-Contact Ultrasound Enhanced Hot-Air Drying Of Pear Slices, Sun Changying, Liu Yunhong, Zeng Ya, Shi Xiaowei, Xi Huihan
Food and Machinery
In order to explore the enhancing effect of contact ultrasound on hot air drying process, ultrasound enhanced hot air drying of pear slices was performed. The effects of different ultrasound powers and drying temperatures on drying characteristics and several quality indicators of pear slices were studied. The results showed that the improvement of hot air temperature and ultrasound power could significantly reduce drying time, and the stronger ultrasound enhanced effects could be achieved at lower temperatures. The BP neural network model was applied to predict the process of ultrasound enhanced hot air drying with high fitting precision. The cavitation and …
Effect Of Salt On Intestinal Microorganism, Enzyme Activities And Blood In Mice, Zhu Jiayuan, Zheng Tao, Xiao Nenqun, Luo Huaihao, Gong Jun, Bao Sihui
Effect Of Salt On Intestinal Microorganism, Enzyme Activities And Blood In Mice, Zhu Jiayuan, Zheng Tao, Xiao Nenqun, Luo Huaihao, Gong Jun, Bao Sihui
Food and Machinery
In order to understand the effect of salt on intestine microorganism, enzyme activities and blood in mice, and to provide scientific basis for daily rational use of salt for human beings, the KM mice were randomly divided into five groups of A, B, C, D, E group, each group with 6 rats of half male and half female. The mice was corresponding gavaged continuously for one month according to the conversion of clinical dose of 0, 3, 6, 9, 12 g, and then the mice was collected eyeball blood to blood routine analysis as well as the contents from jejunum …
Complex Enzyme Hydrolysis Of Ovalbuim And Its Characteristics And Structure Analysis, Liu Lili, Chen Ke, Li Yu, Dai Xiaoning, Meng Yuanyuan
Complex Enzyme Hydrolysis Of Ovalbuim And Its Characteristics And Structure Analysis, Liu Lili, Chen Ke, Li Yu, Dai Xiaoning, Meng Yuanyuan
Food and Machinery
In order to improve the functional characteristics of egg albumin, taking the egg Ovalbumin (OVA) as the research object, the complex enzymes (papain, neutral protease and flavor protease) were used to modify OVA, and its functional and structural properties were analyzed. Taking the degree of hydrolysis (DH) as index, the optimum mass ratio of the complex enzyme (Papain∶neutral protease∶flavor protease) was determined to be 1∶1∶2. Based on the single factor, the enzymolysis conditions were determined by response surface methodology (RSM) and the optimum conditions were pH of 7.5, enzyme hydrolysis time of 7 h, OVA concentration of 5%, enzyme amount …
Sensory Quality Evaluation Model Of Fermented Millet Pepper Based On Intelligent Sensory, Yi Yuwen, Chen Gang, Peng Yiqin, Zhang Xu, Deng Jing, Qiao Mingfeng, Liu Yang, Wang Cong
Sensory Quality Evaluation Model Of Fermented Millet Pepper Based On Intelligent Sensory, Yi Yuwen, Chen Gang, Peng Yiqin, Zhang Xu, Deng Jing, Qiao Mingfeng, Liu Yang, Wang Cong
Food and Machinery
In order to study the quality change of fermented millet pepper, the sensory evaluation model of fermented millet pepper was established using the intelligent sensory instruments (electronic nose and electronic tongue) and sensory evaluation, combinating with the method of correlation analysis, principal component analysis, stepwise regression analysis and fitting test. The results showed that the weight of the simplified sensory indicators were 38.96%, 35.06% and 25.97%, respectively, and the fitting test R2=0.998 indicated that the model can replace the original evaluation method. Taking the PCA of the intelligent sensory index as the independent variable and the sensory evaluation …
Research On Evaluation Method For The Period Of Mentholated Holding In Menthol Filter Rod, Jin Qiang, Sun Qingjie, Zhu Xianyan, Wang Minghui, Sheng Peixiu
Research On Evaluation Method For The Period Of Mentholated Holding In Menthol Filter Rod, Jin Qiang, Sun Qingjie, Zhu Xianyan, Wang Minghui, Sheng Peixiu
Food and Machinery
A comprehensive evaluation method of the aroma quality for menthol filter rod was established by using aroma ratio (B), aroma retention (L) and aroma quality (P) by referring to the flavor assessment methods in the food industry at home and abroad, sensory evaluation methods of cigarette. It was introduced into the evaluation of the period of mentholated holding in menthol filter rod. Through the sensory and quantitative evaluation of the aroma quality for menthol filter rod, the comprehensive score standard for judging the period of mentholated holding in menthol filter rod was determined, and a new method which was scientific, …
Analysis Of Amino Acid Composition And Nutritional Evaluation Of Sichuan White Goose, Zhang Jie, He Hang, Jie Xiaodie, Tian Xu, Cheng Yating, Chen Peiyao, Liu Anfang
Analysis Of Amino Acid Composition And Nutritional Evaluation Of Sichuan White Goose, Zhang Jie, He Hang, Jie Xiaodie, Tian Xu, Cheng Yating, Chen Peiyao, Liu Anfang
Food and Machinery
Sichuan white goose is an excellent local poultry breed. In order to measure its nutritional value, the muscle amino acid composition of distinct body parts for Sichuan white goose of different age and gender was analyzed. In this study, hydrochloric acid hydrolysis was adopted for digestion of muscle protein and Hitachi L-8800 automatic amino acid analyzer was used to determine the content of amino acids. The results showed that 17 amino acids were detected in all muscle samples, among which the content of Glu was the highest, followed by Asp and Leu. We found that, of the three factors, age, …
Valuable Components Analysis And Nutritional Evaluation On Antarctic Krill (Euphausia Superba) Meals, Quan Qinguo, Duan Weiwen, Zeng Xuege, Chen Ming, Zhang Zewei, Hao Jiming, Liu Shucheng, Ji Hongwu
Valuable Components Analysis And Nutritional Evaluation On Antarctic Krill (Euphausia Superba) Meals, Quan Qinguo, Duan Weiwen, Zeng Xuege, Chen Ming, Zhang Zewei, Hao Jiming, Liu Shucheng, Ji Hongwu
Food and Machinery
Key components of Antarctic Krill meals were analyzed such as protein, lipids, minerals and chitin content. Composition and content of amino acids, fatty acids and mineral element were also researched using nutritional assessment strategies in this paper. Finally, the protein processing base material was prepared by two steps include sieving and defatting. The results showed that the content of protein was 61.38%, lipids was 14.49%, chitin was 6.08% and mineral element was 6.27%. Protein of Antarctic Krill meals was a high quality protein referenced by FAO/WHO based on the total amino acid content of 47.34%, essential amino acid content of …
Analysis Of Changes In The Nutrients And The Antioxidant Activities For Rosa Sterilis D.Shi During Development, Xie Guofang, Wang Yubo, Kong Deyin, Wang Lirong, Zhou Xiaoli, Zhao Zhibing, Liu Yongling
Analysis Of Changes In The Nutrients And The Antioxidant Activities For Rosa Sterilis D.Shi During Development, Xie Guofang, Wang Yubo, Kong Deyin, Wang Lirong, Zhou Xiaoli, Zhao Zhibing, Liu Yongling
Food and Machinery
To understand the changes of the main nutrients and antioxidant activities of Rosa sterilis D.Shi during development. The changes of the main nutrients of Rosa sterilis D.Shi, including cellulose during development, reducing sugar, protein, ascorbic acid, total flavonoids, and total polyphenols, were investigated. The antioxidant capacity was analyzed by using the DPPH, ABTS, and FRAP. The antioxidant nutrients were determined by correlation analysis and principal component analysis. The results showed that there were differences in changes of nutrients and antioxidant activities of Rosa sterilis D.Shi during development. The content of cellulose and protein decreased with the development. The content of …
Determination And Comparison Of Hydrolyzed Amino Acids And Free Amino Acids In Six Kinds Of Grape Seeds, Wei Yangfei, Song Hai, Yue Guoren, Zhang Hongxi, Li Caixia
Determination And Comparison Of Hydrolyzed Amino Acids And Free Amino Acids In Six Kinds Of Grape Seeds, Wei Yangfei, Song Hai, Yue Guoren, Zhang Hongxi, Li Caixia
Food and Machinery
High performance liquid chromatography(HPLC) was developed to detect the hydrolyzed amino acids and free amino acids in grape seeds with phenyl isothiocyanate(PITC) as the derivative reagent. For chromatographic analysis, a Thermo AcclaimTM 120 C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of solvent A and B with the flow rate of 1.0 mL/min by gradient elution. Solvent A was sodium acetate buffer solution (pH 6.5) and solvent B was methanol-acetonitrile-water (20∶60∶20, v/v/v). The temperature of column was maintained at 30 ℃. The wavelength was set at 254 nm. The results showed …
Highly Sensitive Detection Of Bisphenol A Based On Fret From Up-Conversion Nanoparticles To Gold Nanoparticles, Xu Zhou, Lu Shizhen, Chen Maolong, Zhu Yingyue, Ding Li, Cheng Yunhui
Highly Sensitive Detection Of Bisphenol A Based On Fret From Up-Conversion Nanoparticles To Gold Nanoparticles, Xu Zhou, Lu Shizhen, Chen Maolong, Zhu Yingyue, Ding Li, Cheng Yunhui
Food and Machinery
The 13 nm gold nanoparticles were synthesized and were modified with Bisphenol A(BPA) aptamer. The oil-solubility Up-conversion nanoparticles (UCNPs) were modified with polyacrylic acid (PAA) to form water-solubility UCNPs. Then water-solubility UCNPs were coated with the complementary DNA strand. Based these nanoparticles, a FRET aptasensor for BPA was successfully fabricated by using Up-conversion nanoparticles as energy donor and gold nanoparticles as energy acceptor. The results show that the detection system has a good linear relationship at 1×10-9～1×10-3 mol/L (R2=0.992 3), and the detection limit is as low as 1×10-10 mol/L. The method is proved …