Food Biotechnology Commons^™

Design And Experiment Of Light Angle Adjustable Device For Measuring Sugar Content Of Citrus By Near Infrared Spectroscopy, Zeng Xianming, Han Longbo, Wen Tao, Dai Xingyong

Food and Machinery

Objective: To solve the problem of insufficient spectral information obtained by the fixed illumination angle of the near-infrared spectroscopy to detect the sugar content of fruits. Methods: A light angle adjustable device for the diffuse transmission near-infrared spectroscopy was designed to detect the sugar content of citrus based on the simplified model of the remote motion center of the light source. The device had carried out the structural performance analysis and the test of the citrus sugar under different illumination angles. Results: The adjustment range of the light angle of the device was set to 0°～60°. The displacement deviation between …

Design And Test Of On-Line Checking Device For Electronic Belt Weigher, Zhang Linfeng, Liu Suijun, Yang Linchao, Liu Ying, Hu Jiacheng, Cai Jinhui, Shen Yinchu, Li Shaohua, Li Qing

Food and Machinery

Objective: In order to prevent the problem of small range electronic belt scale with inefficient method of traditional intermediate verification, low verification accuracy and security. Methods: A on-line intermediate check device was designed for small range electronic belt scale, which was composed of digital transmission control module and automatic loading module. Taking standard weight as input quantity, the upper computer controlled the manipulator to grab the weight onto the transmission belt to implement cyclic loading, then evaluated the measurement results and upload the evaluation results to the cloud. Results: After verification test and data analysis, for electronic belt weigher, it …

Analysis Of Green Walnut Peeling Mechanism And Device Design And Experiment, Li Kang, Li Changhe, Liu Mingzheng, Wang Leyi, Fan Linzheng, Ye Xiangrui, Wang Ziwen, Wang Renkun, Cao Chengmao, Wu Jiankang

Food and Machinery

Objective: To improve the mechanization of the initial processing of walnuts, and to solve the problems such as low stripping rate and high crushing rate in the process of green walnut peeling. Methods: A green walnut peeling device was designed which was integrated scraping and squeezing, rolling and cleaning. The mechanical and kinematic analysis of the peeling process of green walnuts, was made to establish a spatial force model of green walnuts combined with theoretical mechanics theory, and the key structural of system devices was designed. Based on the single-factor test, a three-factor, three-level orthogonal test was conducted with Design-Expert …

Research On The Integration Of Regional Culture Into Food Packaging Design Under The Background Of Cultural And Tourism Integration, Chu Chun

Food and Machinery

Under the background of cultural and tourism integration, the article expounds on the necessity and solution of integrating local culture into local food packaging design. By exploring the logical relationship between local culture and packaging design, analyze successful food packaging design cases in the field of market consumption and cultural communication. It is pointed out that the main pain points of the integration of local culture into food packaging design at present are the breaking of the situation of locality and modernity, the transformation of local cultural heritage resources, the upgrading from daily to gift, and the stylization and rejuvenation. …

Research And Application Progress Of Cold-Chain Logistics Packaging Technology Of Fresh Agricultural Products, Xiang Mingyue

Food and Machinery

Cold chain logistics packaging technology is crucial for the operation of perishable food chains （such as meat, fish, dairy products, fruit and vegetable products） and certain products in the pharmaceutical industry （such as vaccines, biopharmaceuticals, and blood products）. In the future, the cold chain logistics packaging of fresh agricultural products will tend towards high-performance, environmentally friendly, and digital development. The article reviews the packaging technologies used in the cold chain logistics of fresh agricultural products in recent years. Analyzes the characteristics, use value, application scope, technical advantages and disadvantages of different packaging materials, and analyzes the potential cold chain logistics …

Effect Of Ultra-High Pressure Treatment On The Texture, Flavor And Shelf Life Of Cream Cheese, Zhang Yuanyuan, Liu Zhenmin, Zheng Yuanrong

Food and Machinery

Objective: This study aimed to determine the optimal conditions for ultra-high pressure (HHP) cream cheese. Methods: The influence of non-thermal processing pressure on the texture, flavor and shelf life of homemade cream cheese were explored. By using the texture profile analysis (TPA), solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS), and colony counting method, the texture, flavor substances, and microorganisms of cheese were identified and evaluated. Results: After HHP treatment, the hardness, adhesiveness, and resilience of cream cheese decreased. The springiness of cheese reached the highest at 300 MPa, significantly increasing by 14.0% (P<0.05). When the pressure is more than or equal to 400 MPa, the content and species of volatile substances in cheese are significantly reduced. Principal component analysis showed that 200 and 300 MPa treated cheese and unpressurized cheese were both in the first principal component area. After HHP treatment of artificially dyed cream cheese, the number of bacterial colonies decreased significantly (P<0.05), and the greater the treatment pressure, the better the bactericidal effect. When the HHP treatment pressure is greater than or equal to 300 MPa, the shelf life of cheese was effectively extended from 7 days to 21 days. Conclusion: The cream cheese treated by HHP has a good texture and original flavor while effectively extending the shelf life.

The Effect Of Grape Seed Extract Nanoparticles/Pullulan Coatings On The Quality Of Salmon Fillets, Guo Yajuan, Fan Jungang, Li Jianzhen

Food and Machinery

Objective： This study aimed to improve the preservation properties of pullulan （PUL） coating and prepare preservation materials with sustained-release properties. Methods： GSE nanoparticles were prepared by grape seed extract （GSE）, chitosan and γ-polyglutamic acid. GSE nanoparticles of different quality were added to the PUL coating solution to prepare GSE nanoparticles/PUL coatings. The preservation properties of GSE nanoparticles/PUL coatings on salmon fillets were evaluated. Results： Compared with untreated fish fillets, the total number of bacteria in fish meat was reduced, and lipid oxidation and protein decomposition were delayed by 1.08% GSE nanoparticles/PUL coating. In addition, after fish fillets were treated …

Apple Surface Defect Detection Based On Improved Cnn And Data Augmentation, Pi Wei, Qu Xilong, Wang Shaocheng, Li Qingchun

Food and Machinery

Objective: Improve the detection accuracy and efficiency of apple surface defects. Methods: An detection method for apple surface defects was established based on an improved convolutional neural network (CNN) and data augmentation method. Firstly, the classical CNN was improved to detect apple surface defects. Then, using the conditional generation adversarial network, the image data of surface defect free and defective apples was augmented with synthetic apple images to improve the detection performance of the improved CNN for apple surface defects. Finally, by pruning the CNN model, the detection accuracy, detection speed and energy saving limits of apple surface defects were …

Effects Of Hydroxyl Safflower Yellow A On Lipopolysaccharide Induced Depression Model Rats, Yiming Gaghafu, Mian Qiang-Hui, Han Nan-Yin, Abudureyimu Yusufu

Food and Machinery

Objective: This study aimed to investigate the antidepressant effect of hydroxyl safflower yellow A on rats induced by lipopolysaccharide. Methods: The depression model of rats was established by intraperitoneal injection of LPS. The normal control group, the model group, the positive control group and the hydroxyl safflower yellow A groups with different doses were set up, and the depression like behavior of rats was evaluated. The contents of TNF-α, IL-1β, IL-6 and BDNF, 5-HT in serum of rats were determined, and the histopathological changes of hippocampus were observed. The protein expression of TLR/NLR signaling pathway was detected. Results: …

Effects Of Addition Amounts Of Crosslinker And Etherifying Agent On Properties Of Modified Waxy Corn Starch, Zhao Hai-Bo, Sun Chun-Rui, Wang Jin-Peng, Cui Bo, Cheng Yun-Hui, Chen Ling, Zhang Gui-Ming, Xu Ning

Food and Machinery

Objective: This study aimed to control the degree of crosslinking and etherification in the preparation process of crosslinked hydroxypropyl starch and improve the product performance. Methods: Waxy corn starch was used as a raw material to prepare the composite modified starch （hydroxypropyl distarch phosphate, HDP） under alkaline conditions, during which process sodium trimetaphosphate （STMP） and propylene oxide （PO） was used as the cross-linker and etherifying agent, respectively. The effects of the addition level of STMP and PO on the properties of the Brabender viscosity behavior, transparency and freeze-thaw stability of waxy corn starch were investigated. Results: Combined with the preliminary …

Screening, Identification And Biological Characteristics Of Lactic Acid Bacteria Inhibiting Vibrio Parahaemolyticus, Yu Chen, Yang Lu, Guan Feng, Zhang Jie, Yuan Yong-Jun

Food and Machinery

Objective: This study aimed to enrich the functional lactic acid bacteria resource bank and to search for excellent starting strains of lactic acid bacteria with the antagonistic ability of Vibrio parahaemolyticus. Methods: Lactobacillus strains with broad-spectrum bacteriostatic potential were screened by Oxford cup bacteriostatic test, and their biological characteristics were discussed by growth and metabolic performance, gastrointestinal fluid tolerance, salt tolerance, antibiotic sensitivity and bacteriostatic spectrum. Results: Six strains of lactic acid bacteria were screened by using V. parahaemolyticus as indicator bacteria. They were classified into L. paracasei, L. fermentus and L. plantarum by morphological, physiological and biochemical …

Rapid Identification Of Adulterated Camellia Seed Oil By E-Nose Combined With Chemometrics, Guan-Zhao Yi-Zhu, Fang Fang, Wang Yao-Yao, Tian Zi-Jing, Yang Jian-An, Wang Jian-Hui

Food and Machinery

Objective： To establish a rapid qualitative and quantitative method for the adulteration of Camellia seed oil. Methods： The electronic nose technology combined with chemometrics was used to screen the difference variables based on one-way ANOVA. The qualitative model for identification of adulteration types of camellia seed oil was established through principal component analysis (PCA) and discriminant analysis (DA). A quantitative model for identifying the types and degrees of camellia seed oil adulteration was established using orthogonal partial least squares (OPLS). Results： The R² of the model was over 0.98, the RMSEE was lower than 0.005, and the RMSECV was …

Rutin Detection By Electrochemical Sensor Based On Fe3O4@Tio2@Au, Zhou Hong-Yan, Song Yun

Food and Machinery

Objective: A novel electrochemical sensor with high sensitivity was developed for the quantitative analysis of rutin in tartary buckwheat. Methods: A layer of titanium dioxide was loaded on the surface of ferric oxide nano materials (Fe₃O₄) to prepare core-shell Fe₃O₄@titanium dioxide (Fe₃O₄@TiO₂) nano composites, and nano gold (Au) was loaded on its surface for preparing a new type of Fe₃O₄@TiO₂@Au nanocomposites. Then an electrochemical sensor was constructed for quantitative analysis of rutin. The optimal working conditions of the sensor were …

Effects Of Ethanol And Methyl Jasmonate Fumigation On The Quality Of Bamboo Shoots During Storage, Xu Jing-Yang, Ji Ning, Wang Rui, Zhang Ni, Liu Ren-Chan, Wang Si-Yuan, Deng Yun-Bing

Food and Machinery

Objective： This study aimed to provide a basis for the post-harvest storage and preservation of fresh shoots from bamboo (Chimonobambusa quadrangularis). Methods: Fresh bamboo shoots were fumigated with 500 μL/L anhydrous ethanol and 0.1 mmol/L MeJA, and distilled water was used as blank control (CK) to determine the storage period, i. e. temperature (1.0±0.3) ℃ and relative humidity (90±5)%. The respiratory intensity, hardness, browning index, soluble protein, and activity of PAL, POD, PPO and APX were detected. The quality differences of fresh bamboo shoots during storage were analyzed using principal component analysis. Results: Combined fumigation treatment can reduce …

Optimization Of Ginseng Ratio And Semen Ziziphi Spinosae Antioxidant Activity Based On The Combination Of Entropy-Weight Method And Grey-Correlation Degree Method, Xiao Feng-Qin, Wang Xin, Liu Hui, Dong Hong-Ying, Zhou Wen-Wen, Xue Zhi-Jia, Li Guang-Zhe, Yan Ming-Ming

Food and Machinery

Objective： This study aimed to develop relevant health products with ginseng and semen ziziphi spinosae as raw materials. Methods: HPLC and UV spectrophotometer were used to study the chemical components of different compatibility ratios of ginseng and semen ziziphi spinosae. The antioxidant activity in vitro of ginseng and semen ziziphi spinosae with different compatibility ratios was analyzed by DPPH free radical, ABTS⁺free radical, hydroxyl free radical and superoxide anion free radical scavenging rate. Moreover, the grey relational degree method was used, and entropy weight method was used as the discrimination coefficient for statistical analysis, combined with the characteristics …

Effects Of Wet Processing On Coffee Quality, Li Xue-Rui, Liu Xiu-Wei, Li Hong, Yan Jing, Tian Hao

Food and Machinery

Objective: This study aimed to optimize the wet processing of coffee. Methods: The effects of pectinase concentration, temperature and drying method on the contents of flavor precursors and flavor substances in coffee beans were investigated. The quality ranking was obtained by grey correlation analysis and the volatile substances were analyzed. Results: There were significant differences in the contents of protein, crude fat, caffeic acid, caffeine, chlorogenic acid and volatile compounds among different pectinase concentration, temperature, sun drying and heat pump drying. Grey correlation analysis showed that the quality ranking of coffee in the 2% pectinase + 27 ℃+ sun-drying treatment …

Fermentation Process Optimization And Flavor Composition Analysis Of Plum Peppermint Wine, Guo Ming-Yi, Deng Yan, Du Qian-Hong, Wu Hua-Chang

Food and Machinery

Objective: In order to develop and enrich fruit wine categories and diversify sensory levels, plum and peppermint were chosen out as the main materials for fermenting a compound fruit wine. Methods: Single factor test and response surface test were used to analyze the effects of mint addition amount, initial sugar degree, initial pH value and fermentation temperature on the alcohol and quality of plum peppermint wine. The fermentation process was optimized, and its sensory was analyzed. Moreover, the sensory, physical and chemical indexes and volatile components were detected. Results: The optimum fermentation conditions were as follows: peppermint content 18%, initial …

Optimization Of Complex Enzymatic Extraction Process Of Steroidal Glycosides From Cynanchum Auriculatum Royle Ex Wight And Its Hepatoprotective Activity, Song Wan-Yu, Dai Jing, Feng Jin, Li Chun-Yang, Ma Yong-Qiang

Food and Machinery

Objective: Response surface methodology was used to optimize the extraction process of steroidal glycosides from Cynanchum auriculatum Royle ex Wight by complex enzymatic method, and its effects on alcohol dehydrogenase activity and human hepatoma cell HepG2 were analyzed. Methods: Four experimental factors; enzymolysis duration, temperature, compound enzyme dose and pH were used as responses to a single-factor experiment of the Response Surface Methodology on the steroidal glycoside yields, using perennial C. auriculatum as the target material. The alcohol-induced hepatocyte damage model of HepG2 was used to investigate the contents of ALT, LDH, and GSH and changes in cell shape. Results: …

Research Progress In Nutritional Composition And Biological Function Of Grape Seed Extracts, Zhou Xiang-Hui

Food and Machinery

Grape seed extracts, due to its strong antioxidant activity, is commonly used as a nutritional supplement or dietary supplement. It can be used to prevent metabolic diseases such as inflammation, tumors, and atherosclerosis. This article provides an overview of the nutritional composition and biological functions of grape seed extracts, summarizes the latest research findings on the bioactive ingredients in grape seed extracts in terms of antioxidant, anti-inflammatory, anti-tumor, antimicrobial, and anti-atherosclerosis effects, and discusses the shortcomings and future research directions in grape seed extracts research.

Influence Of Overcooking On Food Digestibility And In Vitro Fermentation, Wensheng Ding

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Areas of char or overcooking commonly appear in foods people consume. It has been reported that overcooked food is harmful to human health. However, little research exists on the effect of overcooking on in vitro protein and starch digestibility and gut microbial fermentation. This study aimed to reveal the connection between overcooking and in vitro protein and starch digestibility and gut microbial fermentation. In vitro protein digestibility of an overcooked ground beef patty was almost half that of a standard cooked sample (27 ± 2% versus 48 ± 6%, respectively; p = 0.02). Whole wheat bread protein digestibility was also …

Dry-Aged Beef Flavor Development, And The Effect Of High Levels Of Vitamin-E On Beef Color Stability, Nicolas Herrera

Department of Animal Science: Dissertations, Theses, and Student Research

Palatability and consumer purchasing are essential factors of fresh meat. To meet consumer expectations during purchasing and consumption, fresh beef must maintain desirable cherry-red color at retail and achieve desirable cooked flavor. Dry-aged beef popularity has grown, and identifying methods to ensure quality dry-aged meat is necessary to maintain demand. To understand dry-aged palatability, this dissertation addressed effects of moisture loss and aging time, fundamental factors of dry-aged beef, using bone-in and boneless strip loins. Study two investigated flavor development using cookery methods and internal temperatures in boneless and bone-in dry-aged beef. In study one, increased dry-aging time (creation effect) …

Evaluating Salmonella Cross Contamination In Raw Chicken Thighs In Simulated Post-Chill Tanks, Raziya Sadat

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Salmonella contamination poses a significant risk to food safety, particularly in poultry products and public health. This study aims to investigate the effectiveness of peracetic acid (PAA) treatment in mitigating Salmonella cross-contamination in chicken thighs within simulated post-chill tanks, considering different inoculum concentrations and organic matter (OM) levels.

The experiment involved the use of five distinct Salmonella serovars to inoculate chicken thighs, which were categorized into two groups: higher inoculation level (ca. 7 log CFU/mL) and lower inoculation level (ca, 4 log CFU/mL). These groups underwent treatment with four different solutions, including 540 ppm PAA with 1.5% OM, 540 ppm …

Preparation Of Xanthoceras Sorbifolia Bunge Shell Biochar Activated By Phosphoric Acid And Its Adsorption Performance For Methylene Blue, Chen Yan-Qin, Wang Xue-Li, Song Shi-Fang, Liu Zun-Qi

Food and Machinery

Objective: The adsorption performance and mechanism of Xanthoceras sorbifolia shell biochar activated by phosphoric acid for methylene blue was investigated in this study. Methods: With phosphoric acid as activator, the Box-Behnken center combination design was used to optimize the preparation conditions of biochar from Xanthoceras sorbifolia shell. The biochar obtained under the optimal preparation conditions was used to adsorb methylene blue in water. Through investigating the adsorption influencing factors, the adsorption characteristics of biochar on methylene blue were determined, and combined with the analysis of kinetic, the adsorption mechanism was discussed. Results: The optimum technological conditions for preparing biochar from …

Extraction And Functional Characteristics And Structural Analysis Of Porcine Liver Salt Soluble Protein, Wang Hao-Yang, Liu Li-Li, Cheng Wei-Wei, Xu Bao-Cheng, Su Ke-Nan

Food and Machinery

Objective： Optimize the preparation process of salt-soluble porcine liver protein and improve the utilization rate of porcine liver by-products. Methods： Taking the extraction rate as the index, the effects of NaCl concentration, pH, extraction time and solid-liquid ratio on the extraction rate of SSLP were analyzed, and the optimal preparation process was determined by orthogonal rotation optimization test. Taking water soluble liver proteins （WSLP） as the control, the functional characteristics （solubility, foaming, foam stability, emulsification, emulsification stability, etc.） of SSLP were compared and analyzed, and Fourier transform infrared spectroscopy （FT-IR） was used, the structural changes were analyzed by low field …

Study On Metabolic Changes Of Crucian Carp Induced By Phoxim Based On 1H-Nmr Metabolomics, Li Hong, Li Dan, Li Li-Juan, Wu Ni

Food and Machinery

Objective: Standardize the use of phoxim to ensure the safety of aquatic products. Methods: Taking freshwater crucian carp as the research object, Selecting phoxim, an organic phosphorus pesticide often used in agriculture, as the representative of exogenous environmental pollutants. The changes in normal physiological metabolism of crucian carp exposed to phoxim were studied by using the ¹H-NMR metabonomics method combined with PCA, PLS-DA, OPLS-DA and other multivariate statistical methods. Based on this, the toxic effects of phoxim on crucian carp and the toxicological mechanism of phoxim in the crucian carp were deduced. Results: Compared with the control group, the …

Study On Antioxidant Properties Of Glycosylated Products Of Wheat Germ Protein, Niu Qiu-Yun

Food and Machinery

Objective: This study aimed to investigate the antioxidant properties of wheat germ protein glycosylation product. Methods: The glycation product (WGP-G) of wheat germ protein (WGP) and glucose was prepared by ultrasonic assisted moist heat method. Different ultrasonic time (10, 20, 30, 40, 50 and 60 min) and reaction temperature (70, 80 and 90 ℃) were measured. The browning degree, grafting degree, reducing power, hydroxyl radical scavenging capacity, superoxide anion radical scavenging capacity, DPPH radical scavenging capacity and ABTS+ radical scavenging capacity of WGP-G at 90 ℃ were evaluated comprehensively. Results: The browning degree of WGP-G increased with the increase of …

Process Optimization And Kinetics Of Arabinose Preparation By Dilute Acid Hydrolysis, Tian Long, Tong Fen-Fei

Food and Machinery

Objective: Preferred biomass hydrolysis for preparation of arabinose and its kinetic analysis during the hydrolysis process. Methods: Single factor experiment and response surface methodology were used to optimize the conditions for the preparation of arabinose from gum Arabic by dilute acid hydrolysis, and the kinetics of the hydrolysis process was studied. Results: The optimum hydrolysis conditions were as follows: reaction time 237 min, sulfuric acid concentration 0.16 mol/L, reaction temperature 91 ℃. Three parallel experiments were carried out under these conditions. The average concentration of L-arabinose in the hydrolysate was 35.45 g/L. The kinetic parameters of arabinose formation and …

Analysis Of Heavy Metal Content And Health Risk Assessment Of Astragalus Membranaceus In Gansu Province, Zhang Si-Wei, Li Dong-Mei, Liu Xiao-Yun, Xue Li-Xin, Sun Jian-Yun, Li Yong-Jun

Food and Machinery

Objective: This study aimed to investigate the pollution of heavy metals in Astragalus membranaceus in the main producing areas of Gansu, and to evaluate the potential health risks of edible Astragalus membranaceus. Methods: The contents of five heavy metal elements Pb, Cd, As, Hg and Cu in Astragalus membranaceus were determined by inductively coupled plasma mass spectrometer （ICP-MS） by random sampling method, and the data were analyzed. Estimated Daily Intake （EDI）, Target Hazard Factor （THQ） and Lifetime Carcinogenicity Risk （LCR） were used for health risk assessment. Results: The average contents of Pb, Cd, As, Hg, and Cu elements in …

Application Of Dna Barcoding Technology In The Identification Of Adulterated Ingredients Of Edible Blood Products, Li Jiong, Wu Qiong, Jiang Hai, Hu Ming-Jie, Xu Xin-Yi

Food and Machinery

Objective: A COI sequence-based full-length DNA barcoding technology was established to identify adulteration of seven animal-derived components （including pigs, cows, sheep, chickens, ducks, geese and rabbits） in edible blood products. Methods: After the blood products were extracted and purified by DNA extraction and PCR amplification, the clone sequencing results were submitted to the DNA barcode local database of the 7 blood products for sequence comparison. The pretreatment method, DNA extraction method and PCR amplification conditions of the blood products were also selected and optimized, and various common blood product adulteration models were established to study the lowest adulteration detection rate …

Construction And Application Of Multi-Dimensional Food Safety Evaluation Index System Model, Miao Lu, Zhu Ke, Cao Yi-Qun, Zhou Mo-Qin, Gao Meng-Zhao, Tuo Xiong-Xin, Deng Ke

Food and Machinery

Objective: It was the method of evaluating regional food safety status through food safety evaluation index model. Methods: In the index model, various factors affecting food safety, such as the size of the city, the types of food, the hazards of the test items, the place of production, etc., were comprehensively considered, and a variety of data sources were used to support the model. Using this model, the results of food safety index could be calculated from multiple angles and different levels, such as region, food category, region and food category. Results: Sampling data of Guangxi food safety assessment in …