Food Biotechnology Commons^™

Optimization Of Macroporous Resin Adsorption Process Of Enzymatic Hydrolysis Of Pueraria Protein And Its Antioxidant Activity In Vitro By Response Surface Method, Yue Yihan, Yan Mingming, Shao Shuai, Ding Yiyuan, Jiang Ting

Food and Machinery

Objective： This study aims to optimize the adsorption of enzymatic hydrolysis of Pueraria protein by macroporous resin and maximize the antioxidant properties in vitro. Methods: The Box-Behnken response surface method was used to determine the optimization of adsorption of enzymatic hydrolysis of Pueraria protein. Using V_C as a control, the antioxidant activity of Kase was determined before and after purification under the optimal purification process. Results： The optimal adsorption-desorption process of macroporous resin is: the mass concentration of the sample solution was 10.0 mg/mL, the flow rate of the eluent was 2.6 mL/min, and the volume fraction of …

Lactic Acid Fermentation Of Papaya Juice Using Single And Mixed Lactic Acid Bacteria, Zhou Yao, Li Juan, Jia Fengxia, Huang Jingyu, Su Zhimin, Chen Gang

Food and Machinery

Objective: Phytochemistry, microbiology, flavor and functional properties of fermented papaya juice were examined in relation to Lactobacillus plantarum (Lp), Lactobacillus rhamnosus (Lr), Lactobacillus acidophilus (La) and their mixed strains (Lp+Lr、Lp+La、Lr+La), in order to develop a probiotic papaya beverage with improved functionality and health-promoting properties. Methods: In thisstudy, changes in pH, total soluble solids, titratable acid, viable cells, glucose, fructose, sucrose, organic acids and volatile compounds were determined. The contents of total phenolics and total flavonoids, as well as the inhibitory activity against α-glucosidase and tyrosinase enzymes during the fermentation process, were also investigated. Results: The single-and mixed strain cultures …

Effect Of Ultra-High Temperature Transient Sterilization On Quality Of Rice Wine, Xiong Sirui, Wang Longyu, Wang Yunfeng, Wang Yuting, Jin Tieyan, Zhang Ling

Food and Machinery

Objective: To study the effect of ultra-high temperature (UHT) on the quality of rice wine. Methods: Korean rice wine underwent weekly measurements of its physicochemical, sensory, and microbiological parameters during the storage period after being subjected to UHT. This allowed for the analysis of changes in the quality and functional components of the sterilized Korean rice wine after 20 weeks of storage, as well as the determination of the best sterilization conditions for this method. Results: Sterilization time of 5 s at 125 ℃ is the optimum parameter for UHT. After 20 weeks of storage, the total amino acid content …

Research Progress On Emulsifying Properties And Modification Methods Of Rice Bran Protein, Ou Wenhua, Cao Tianxiang, Zhang Dongmei, Gu Lihao, Zhang Wanping, Jiang Wen

Food and Machinery

Natural rice bran protein has poor emulsifiability, which limits its application in food industry, so it is necessary to adopt appropriate methods to modify it. In this review, the composition, emulsifying properties and modification of rice bran protein were summarized. The conclusion suggested that the emulsifying properties of rice bran protein could be improved by improving the modification technology of rice bran protein or by blending different high technologies, to expand the development and utilization of rice bran protein and accelerate the application process of rice bran protein in food and cosmetics.

Application Of Surface-Enhanced Raman Spectroscopy In Food Safety Detection, Wang Liuliu, Sun Fangtao

Food and Machinery

Based on Raman scattering phenomenon, surface-enhanced Raman spectroscopy （SERS） detection technology enhances "fingerprint" information of tested substance by adsorbing it on the surface of SERS substrate to achieve trace detection. With the advantages of simple pre-processing, high sensitivity, simple operation, real-time non-destructive detection on site and so on, SERS analysis technology has attracted much attention in food safety rapid detection. The characteristics and limitations of various types of SERS substrates were introduced. The recent research on SERS detection technology in food safety detection was summarized in detail. Finally, this paper discussed the challenges of its commercial application, and prospected the …

Research Progress In The Detection Technology Of Chloramphenicol Residues In Animal Derived Food, Yuan Zhe, Peng Maomin, Liu Li, Peng Xitian, Xia Hong

Food and Machinery

Chloramphenicol is a widely used bactericide and highly effective antibiotic in aquaculture and livestock breeding. To protect human health, detecting chloramphenicol residues in animal-derived food products has become increasingly important. This review introduces the properties of chloramphenicol, the limit standards, and the hazards of residual chloramphenicol in animal-derived food products. Furthermore, it describes commonly used chloramphenicol detection methods. In addition, the review thoroughly analyzes and summarizes the advantages and disadvantages of different detection methods, outlining methods to improve the efficiency and precision of chloramphenicol detection to ensure the quality and safety of animal-derived food products. Finally, the prospects for developing …

Research Progress Of Plant Exosome-Like Nanoparticles On Characteristics, Components And Functions, Chen Chunping, Xu Hongyan, Liu Shuaichen, Tian Yuxin, Wang Yuchen

Food and Machinery

Exosome-like nanoparticles （ELNs） are nano-sized extracellular vesicles enclosed by lipid bilayer membrane and secreted by cells; And carrying a variety of cargo, including lipids, proteins, miRNAs and secondary metabolites. Plant ELNs have received widespread attention due to their unique structure and excellent physiological activity. This review summarizes the isolation methods, characteristics, components and functions of plant ELNs, focusing on the application of multi-omics technologies in plant ELNs. In addition, some suggestions for further study of plant ELNs were put forward.

Optimization Of The Extraction Process And Molecular Mechanism Of Inhibition On Tyrosinase Of Kojic Acid, Fang Jingjie, Chen Jian, Chen Yajuan, Liang Ying

Food and Machinery

Objective: This study aimed to develop new, efficient, safe and precise tyrosinase inhibitors. Methods: The orthogonal test was used to optimize the extraction conditions of kojic acid. The inhibitory effect of kojic acid on tyrosinase was studied by enzyme kinetics, and the mechanism of its interaction with tyrosinase from different sources was analyzed by molecular docking technology. Results: The optimum process conditions for extracting kojic acid were as follows: activated carbon content of 5%, decolorization temperature of 75 ℃, pH 2.0, decolorization time of 50 min, and the purity of kojic acid was 97.85%. the IC₅₀ of kojic acid …

Rapid Detection Of Methyl Parathion By Electrochemical Biosensor Based On Rgo-Aunps Modified Screen Printing Electrode, Geng Junhao, Li Xuezhi, Zhou Jianping, Chen Changhua

Food and Machinery

Objective: In order to achieve rapid detection of methyl parathion. Methods: An acetylcholinesterase sensor was prepared based on gold nanoparticles and reduced graphene oxide for quantitative detection of Methyl parathion. Reduced graphene oxide, gold nanoparticles and acetylcholinesterase were successively modified on the surface of the screen printing electrode by layer-by-layer assembly method. The catalytic activity and impedance characteristics of the sensor, the relationship between the sensor's inhibition rate and MP concentration, and the actual sample detection were evaluated. Results: The prepared acetylcholinesterase biosensors showed excellent affinity for acetylthiocholine chloride with the Michaelis-Menten constant of 2.76 mmol/L. Under the optimal conditions, …

Determination Of Chlorantraniliprole And Penoxsulam Residues In Rice By Quechers-Uplc-Ms/Ms, Huang Zijie, Huang Pengmian, Luo Kuanneng, Xiang Zhongping, Zhang Honggang

Food and Machinery

Objective: To establish a new QuEChERS-ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the detection of chlorantraniliprole and penoxsulam residues in rice. Methods: After the sample was extracted with 0.2% formic acid-acetonitrile, purified with N-propylethylenediamine (PSA) and graphitized carbon black (GCB) packing, 0.2% formic acid water and acetonitrile were used as mobile phases for gradient elution, and then subjected to C₁₈ chromatography. UPLC-MS/MS was used to column separation and determination. Results: The limits of quantification (LOQ) of chlorantraniliprole and penoxsulam were both 0.004 mg/kg, and the method detection limits (LOD) were both 0.001 mg/kg. Chlorantraniliprole and penoxsulam had …

Fruit Variety And Freshness Recognition Method Based On Yolo-Ffd, Yan Zi, Chen Liangyan, Liu Weihua, Lai Huaqing, Ye Sheng

Food and Machinery

Objective: In order to improve the situation that existing fruit recognition and classification methods rely on manual operation and complex equipment. Methods: A lightweight model YOLO-FFD (YOLO with fruit and freshen detection) was proposed, which based on the YOLOv5 framework. Firstly, LightweightC3 was designed as the basic unit of the backbone feature extraction network based on the depth separable convolution and GELU activation function, which reduced the number of model parameters and computation, and speeds up the convergence of the model. Secondly, EnhancedC3, a large kernel depth separable convolution module, was used to improve the neck of the original model, …

Compression Characteristics And Damage Mechanism Of Rose Orange, Lu Siyu, Luo Qiang, Lai Yushu, Xia Qiming

Food and Machinery

Objective: Reduced the damage of rose orange in the process of packaging and transportation, and provided a theoretical basis for postharvest storage and spectral quality testing. Methods: According to the basic geometric data of rose orange and the maximum compression force and puncture force obtained from TPA test and puncture test, the finite element model was constructed to simulate the compression of rose orange. Results: The diameter of rose orange ranged from 58.2 mm to 78.6 mm, the average weight was 169.746 g, the average thickness of peel at the equator was 3.1 mm, the maximum puncture force was 6.26 …

Preparation, Structural Characteristics And Antioxidant Activity Of Cardamine Violifolia Oligopeptides, Wen Xiaolong, Xiang Jiqian, Zhang Liang, Li Yajie, Long Lan

Food and Machinery

Objective: This study aimed to prepare oligopeptidess of Camelina violifolia and expand its application range. Methods: C. violifolia protein was hydrolyzed by alkaline protease, and then used ultrafiltration membrane for isolating and concentrating, and obtained oligopeptides by freeze-drying. The structure and antioxidant activity of C. violifolia oligopeptides were analyzed. Results: The optimum conditions for the hydrolysis of C.violifolia protein were as follows: hydrolysis temperature 50 ℃, pH 10, enzyme dosage 4%, substrate concentration 2%, and hydrolysis degree 17.9%. The C. violifolia oligopeptides contents of protein and polypeptide were 49.01% and 42.87%, respectively. The oligopeptides had a strong absorption band at …

Review Of Caenorhabditis Elegans In Food Nutritional Function Evaluation, Chen Wang, He Hailun, Zheng Yanjun, Yi Cuiping

Food and Machinery

As an ideal model organism, Caenorhabditis elegans has been increasingly used in the evaluation of the nutritional function of food. Compared with other models such as cells and mice, C. elegans have the advantages of small size, short lifespan, high genetic homology with humans, easy observation of research results, and large batch culture in the laboratory. Therefore, the article summarizes the research methods, potential mechanisms and research progress of C. elegans in food nutritional function such as anti-obesity, antioxidant and anti-aging. In order to provide a reference for new methods in the evaluation of food nutrition function.

Research Progress On Polyphenols In Chestnut Rose Fruit, Yin Yanyan, Zhang Jian, Lu Lunwei, Li Lulu, Zhang Deqin

Food and Machinery

Chestnut rose fruit, known as "Three King fruits", has high edible value and medicinal value. Polyphenols are the second major functional component of chestnut rose fruit with pharmacological and health functions such as antioxidant, regulation of glucose and lipid metabolism, anti-inflammatory and bacteriostatic, antialcoholism and liver protection, and have great potential for food development. This review summarized the research progress of the classification, extraction, qualitative and quantitative analysis, and bioactivity of chestnut rose fruit polyphenols in recent years and prospected the future development direction.

Simulation Analysis Of The Influencing Parameters On The Shelling Efficiency Of Rubber Roller Husker Based On Adams, Wang Zixiao, Wang Wangping, Song Shaoyun

Food and Machinery

Objective: Improve the hulling efficiency of the rice huller. Methods: Simulated the process of rice hulling, and analyzed the influence of various parameters on the hulling process by changing the movement speed of the rubber roller and the line speed difference. Firstly, the model of huller and the model of single-sided shelled brown rice were established in SolidWorks, and then the established model was imported into ADAMS for simulation, and the effects of the corresponding factors on the hulling efficiency of huller were observed by changing the rotational speeds of the fast and slow rollers, the difference in line speeds …

Development Of Fungal Hydrogels For 3d Bioprinting And Cellular Agriculture, Lauren E. Doyle

Theses and Dissertations--Biosystems and Agricultural Engineering

Hydrogel scaffolds are an important component to cellular agriculture. There is a need for a sustainable alternative to the commonly used gelatin-sodium-alginate hydrogels. Specific edible fungus species may provide a suitable alternative. Many fungi possess high protein contents, ease of cultivation, and strong functional properties. However, functional characterizations and application of fungal flour and protein extracts in cellular agriculture are lacking in the literature. Therefore, this study’s objectives were to 1) Develop processes to create functional fungal protein fractions from different fungal strains and evaluate their characteristics, and 2) Quantify the printability of the resulting fungal gels for the creation …

Reducing Food Scarcity: The Benefits Of Urban Farming, S.A. Claudell, Emilio Mejia

Journal of Nonprofit Innovation

Urban farming can enhance the lives of communities and help reduce food scarcity. This paper presents a conceptual prototype of an efficient urban farming community that can be scaled for a single apartment building or an entire community across all global geoeconomics regions, including densely populated cities and rural, developing towns and communities. When deployed in coordination with smart crop choices, local farm support, and efficient transportation then the result isn’t just sustainability, but also increasing fresh produce accessibility, optimizing nutritional value, eliminating the use of ‘forever chemicals’, reducing transportation costs, and fostering global environmental benefits.

Imagine Doris, who is …

Purification And Comparison Of Enzymatic Properties Of Endogenous Transglutaminase Between Silver Carp And Black Carp, Yi Lin, An Yueqi, Liu Ru, Hu Yang, Xiong Shanbai

Food and Machinery

Objective: This study aimed to investigate the differences in enzymatic properties of endogenous transglutaminase （TGase） in silver carp and black carp. Methods: STG and BTG were purified from the muscle of silver carp and black carp, respectively, by 80% ammonium sulfate precipitation, Q-Sepharose FF, and Sephacryl S-200 HR chromatographies. Two enzymes were analyzed for relative molecular weights, peptide sequences, secondary structures, optimal reaction conditions, and thermal inactivation kinetics. Results: The purified STG and BTG showed similar relative molecular weights, of which the enzyme activities were 14.34 U/mg and 12.67 U/mg, respectively. Both enzymes showed differences in peptide sequences. The secondary …

Screening And Identification Of Bifidobacterium Longum From Maternal Milk And Its Domestication Of Oxygen-Domestication, Zhang Feng, Hou Xinyue, Guo Liqiong, Liu Chunhua, Lin Junfang

Food and Machinery

Objective: To screen Bifidobacterium from breast milk and improve its oxygen tolerance under aerobic conditions. Methods: Dilution and spread plate methods were performed to separate and screen Bifidobacterium from breast milk. Their Bifidobacterium identifications were characterized by 16S rDNA sequencing. The oxygen tolerant domestications were conducted through gradual increase of oxygen pressure and alternation of aerobic and anaerobic cultivations. Results: A novel Bifidobacterium strain was isolated from human milk and was identified as Bifidobacterium longum by 16S rDNA sequencing, which was named as MEFZ-2201. The homology between MEFZ-2201 and model strain (accession number in NCBI: ON631733.1) reached 100%. After oxygen …

Effect Of Different Processing Temperature On Texture Profile And Flavor Of The Edible Bird's Nest, Shang Kexin, Qiu Shuang, Zhang Lan, Cheng Xiangrong, Wang Dongliang

Food and Machinery

Objective: This study aimed to investigate the effects of different processing temperature on texture profile and flavor of dried edible bird's nest. Methods: With dried edible bird's nest as the main raw material, the texture profile analysis of edible bird's nest at different processing temperature was carried out by physical property analyzer. The volatile components of edible bird's nest at different processing temperature were determined by electronic nose and solid phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS). Results: It was found that the adhesion of cooked edible bird's nest decreased significantly (P<0.01), but its resilience increased significantly (P<0.05) with the increase of temperature. 12 kinds of effective flavor substances were detected in the cooked edible bird's nest by SPME-GC-MS, including: 3 alcohols, 2 aldehydes, 2 esters, 3 ketones, 1 acid, and 1 ether, accounting for 6.23%, 49.34%, and 54.85% of the total detected substances in the cooked edible bird's nest at 95 ℃, 105 ℃ and 115 ℃, respectively. Conclusion: Different processing temperature affects the taste of edible bird's nest and the overall odor profile after processing. Moreover, higher cooking temperature tends to make the egg white-like flavor stronger. The volatile substances in the bird's nest stew at 95 ℃ are mainly acids, the bird's nest cooked at 105 ℃ mainly produces alcohols, and the edible bird's nest cooked at 115 ℃ mainly produces ketones and alcohols.

Analysis Of Volatile Flavor Components And Characteristics Of Dough Fermented With Flavor Fermentation Liquid, Zhang Yanmei, Zhu Yuchang, Yang Dandan, Pan Xufang, Bai Hui, Zhou Dazhai

Food and Machinery

Objective: This study aimed to investigate the varieties and contents of volatile components in fermented dough with flavor fermentation broth at different fermentation time. Methods: Headspace solid phase microextraction-gas chromatography (HS-SPME-GC-MS) was used to determine the volatile flavor substances in the dough of the flavor ferment at 12, 24, 36, 48 and 72 h. Also, sensory evaluation of the odor and determination of pH value, total acidity (TAA) and rheological properties of the dough were measured. Results: The strength of eight odors (frankincense, cheese, flower, fruit sweet, wine, malt, grass and sour) showed different performance, depending on the types and …

Effects Of Thawing Methods On The Quality, Physicochemical Properties And Microstructure Of Pork, Wang Li, Chen Wei, Wang Yuanliang, Li Zhongjun, Xia Di

Food and Machinery

Objective: To investigate the best defrosting method for frozen pork. Methods: Investigated the effects of three thawing methods, namely low-frequency electric field thawing, air thawing and hydrostatic thawing, on the juice loss rate, moisture content, water holding capacity, pH, colour difference, volatile saline nitrogen, total bacterial colony, textural characteristics, fat oxidation and protein oxidation of pork after thawing. Results: The low frequency electric field thawing resulted lower juice loss (1.09%), shear force (2 600.15 N), increase in thiobarbituric acid value (TBARS) (62.50%), good colour, pH close to that of fresh meat, less protein oxidation, richer taste material, less damage to …

Spin-Flash Drying Characteristics And Kinetic Model Of Antarctic Krill, Tan Jiayu, Ouyang Jie, Ma Tiantian, Shen Jia

Food and Machinery

Objective: The purpose of this study was to investigate the drying characteristics and establish the kinetic model of Antarctic krill during spin-flash drying. The drying experiments were carried out at 120 ℃ to 180 ℃. Methods: Six common thin-layer drying models were selected to fit the experimental data. Further, the equations of drying model constant and temperature were established to obtain the model expression and validate the optimal drying model. The effective moisture diffusion coefficients were calculated by Fick's second law. Results: The results showed the drying temperature had a significant effect on drying thermal efficiency and drying rate. The …

Preparation Of Matrix Reference Material For Carbaryl Residues In Chinese Yam Flour, Huo Siyu, Gen Xuhao, Yu Libo, Chen Dongdong, Peng Tao

Food and Machinery

Objective: This study aimed to establish a method for the Preparation of matrix reference material for carbaryl residues in Chinese yam flour. Methods: Yam powder containing menafyl was prepared by homogenized, freeze-dried, ground, sifted, mixed and packaged. A liquid chromatography-series mass spectrometer was used for detection, and the isotope internal standard curve method was used for quantification. Through the process of equalization stability evaluation, cooperative setting and uncertainty evaluation, the matrix reference material of the expected yam powder was obtained. Results: The property value and expanded uncertainty of carbaryl was (1.04±0.10) mg/kg (k=2). Conclusion: It could be used …

Determination Of Eight Anions In Liquid Milk, Wu Ziling, Yao Xiaoqing, Shen Yingtao, Huang Jiale, He Minheng

Food and Machinery

Objective： A rapid method was established for the simultaneous determination of variety anions in liquid milk. Methods： The macromolecular protein in liquid milk was precipitated by acetonitrile, and a variety of anions were extracted. After purification by C₁₈ column, the material was separated by Dionex IonPac AS19-HC ion column with gradient elution of potassium hydroxide and conductivity detection. Finally, a method for the determination of bromate, thiocyanate, nitrite, nitrate, chlorate, perchlorate, phosphate and iodine ion in liquid milk was established using ion chromatograph. Results： The results showed that the correlation coefficients （R²）of eight kinds of anion …

Determination Of 17 Aldehydes In Baijiu （Chinese Liquor） By Direct Injection Gas Chromatography, Luo Yue, Zhong Ciping, Huang Ping, Chen Yanmei, Tang Jing, Yu Xiaoqin

Food and Machinery

Objective: To explore the content of aldehydes in Baijiu. Methods: A method for the determination of 17 aldehydes in Baijiu was established, and the methodological performance indexes were studied using statistics. Results: Through statistical analysis, the detection limit of 17 aldehydes in Baijiu was determined to be 0.7～5.9 mg/L, the limit of quantification was 1.9～14.6 mg/L, which passes the quality inspection of the calibration curve within the range of 0.6～320.0 mg/L, and the linear relationship meets the requirements. The recovery rate of intermediate standard addition in Baijiu was 73%～114%, the coefficient of variation in the laboratory was ≤11%, and the …

Solid Phase Extraction-Gas Chromatography-Flame Ionization Detection （Spe-Gc-Fid） For The Detection Of Mineral Oil Saturated Hydrocarbons （Mosh） In Infant Formula, Tang Wanli, Feng Yanying, Xiang Jun, Qin Haijiao, Hu Lingli

Food and Machinery

Objective: To establish a new accurate method for the determination of mineral oil saturated hydrocarbon （MOSH） in infant formula by solid phase extraction combined with gas chromatography-Flame Ionization Detection （SPE-GC-FID）. Methods: Different extraction methods such as soaking extraction, oscillating extraction, ultrasonic assisted extraction, liquid-liquid extraction were used to extract MOSH from infant formula milk powder, and different extraction solvents such as n-hexane, isooctane, benzene, mixed solvents were used to extract MOSH. The optimal extraction method and extraction solvent were determined based on the extraction amount of MOSH. The target compounds purified by AgNO₃ SPE column, and finally detected by …

Determination Of 5 Kinds Of Biogenic Amines In Cheeses By High Performance Liquid Chromatography-Tandem Mass Spectrometry, Zhang Hui, Cao Hui, Wang Jin, Xiao Gongnian, Jiang Kan

Food and Machinery

Objective: A new technical idea was provided for the study of biogenic amine formation mechanism and food safety risk assessment. Methods: An analytical method was established for determination of tyramine, phenylethylamine, tryptamine, octopamine and cadaverine in cheeses with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Biogenic amines were extracted by ultrasonic-assisted solvent extraction from cheeses, and were separated by HILIC column, determined by positive electrospray ionization (ESI) with multiple reaction monitoring (MRM) mode, quantified by the internal standard method. The effect of extraction solvent type, ultrasonic time and matrix effect on the peak area was studied, and the performance of …

Numerical Simulation Of Factors Affecting Flow Characteristics Of Double-Circular-Arc Gear Pump, Huang Cheng, Xu Kai, Wei Zhibo, Li Geqiang

Food and Machinery

Objective: Analyzed the influencing factors of the internal flow field pulsation characteristics of the new double circular arc gear pump, and adopt reasonable parameters to improve the output efficiency of the gear pump. Methods: The two-dimensional flow field simulation model of the gear pump was established, and the computational fluid dynamics software Fluent was used for simulation analysis to study the influence of different tip clearance, different gear eccentricity, load and speed on the instantaneous flow, flow pulsation and flow field characteristics of the oil outlet of the gear pump. Results: As the tooth tip clearance increased, the outlet flow …