Food Science Commons^™

Predictive Modeling Of Listeria Monocytogenes Growth In Ready-To-Eat Meat Products, Manjari Singh

Graduate Theses and Dissertations

Listeria monocytogenes remains a significant food safety concern in ready-to-eat (RTE) meat and poultry products due to its persistence under refrigerated and stressed conditions. Predictive models play a vital role in risk assessment and food safety management; broth models are highly conservation & results in overprocessing. This thesis focuses on developing and validating meat-specific predictive models for Listeria monocytogenes growth and lag for RTE beef, pork, and poultry products. Matrix-specific secondary models were built to predict the maximum specific growth rate (μmax) of Listeria monocytogenes under varying environmental conditions, including temperature, pH, water activity, salt, organic acids, and nitrites. Growth …

Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies, Xu Shi

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Heart failure (HF) is a leading cause of morbidity and mortality worldwide, placing substantial clinical and economic burdens on society. Although advances in therapy have improved patient outcomes, the biological mechanisms that drive HF onset and progression remain incompletely understood. Increasing evidence suggests that the gut microbiota, the trillions of microorganisms residing in the gastrointestinal tract, plays a key role in some forms of heart disease, especially cardiovascular disease. Moreover, gut microbial dysbiosis has been associated with systemic inflammation, metabolic imbalance, and immune dysregulation, all of which contribute to the pathophysiology of HF. Cardiomyopathy, a primary disorder of the heart …

Development Of Databases For Glycan Utilization And Anti-Prokaryotic Defense In Microbiomes, Yuchen Yan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Microbiomes in humans, animals, plants, and other environments are necessary components of the biosphere for global nutrient cycling and maintaining ecosystem balance. In particular, the gut microbiome plays an essential role in modulating human health through glycan utilization. Carbohydrate-active enzymes (CAZymes), CAZyme gene clusters (CGCs), and polysaccharide utilization loci (PULs) are key genomic features that determine microbial capacities for carbohydrate metabolism. Meanwhile, the arms race between phages and prokaryotes has led to the evolution of various anti-prokaryotic immune systems (APIS) in phages to counteract prokaryotic immune systems (PIS).

As bioinformatics methods used to analyze microbiome information advance rapidly, there is …

Dual-Stage Ultrasound Application For Rice Bran Protein Extraction: A Novel Method To Improve Yield, Functional Properties, And Nutritional Profile, Saydul Md Safwa, Nikitha Modupalli, Md Mahfuzur Rahman

Food Science Faculty Publications and Presentations

While ultrasound-assisted protein extraction is well-studied, the application of ultrasound in both the extraction and precipitation stages has not been explored. This study explored the ultrasound (U)-assisted alkaline extraction (AE) at pH 9.5 and ultrasound-assisted isoelectric precipitation (UIP) at pH 3.5 on the rice bran protein yield, functionality, and nutritional quality. The UAE and conventional precipitation (UAE-IP) yielded the highest protein yield of 65.7 %, while conventional extraction yielded 38.9 %. FTIR showed that UAE-IP increased beta-sheets by 96.8 % while AE-UIP decreased the alpha-helices by 42.4 %. Although UAE-IP showed no significant change in surface hydrophobicity, UAE-UIP decreased it …

A Novel Drying Technology Based On Supercritical Carbon Dioxide To Enhance The Functional Properties Of Red Lentil Proteins, Niveditha Asaithambi, Arda Tuhanioglu, Ali Ubeyito

Food Science Faculty Publications and Presentations

Red lentils are protein-rich sources that are highly regarded in the plant-based food industry for their color. Appropriate drying methods are essential for maintaining the physicochemical and nutritional properties of the proteins. This study investigates the impact of three drying methods, namely oven drying, freeze-drying, and supercritical carbon dioxide (SC-CO₂) drying, on the physicochemical and functional properties of red lentil proteins (RLP). SC-CO₂ drying was optimized at varying temperatures (35–55 °C), pressures (10–20 MPa), and CO₂ flow rates (2–3 L/min), with optimal drying observed at 55 °C, 20 MPa, and 3 L/min, resulting in significantly reduced moisture content (5.7 %). …

Effect Of Peracetic Acid Dip Frequency On The Quality And Functional Properties Of Non-Marinated And Marinated Broiler Tenderloins, Joaquin Jose Esquivel Vergara

Graduate Theses and Dissertations

The poultry industry continues to balance the need for effective antimicrobial interventions with the preservation of meat quality. Among the most widely used antimicrobial agents, peracetic acid (PAA) has proven effective for reducing foodborne pathogens in poultry products. However, excessive or repeated exposure to PAA can influence meat functionality by altering protein structure, color, and water-holding capacity. Therefore, the broad objective of this study was to evaluate the effect of PAA dip frequency and post-treatment marination timing on the physicochemical and functional quality of broiler tenderloins (Pectoralis minor), in order to provide processing insights that optimize both microbial safety and …

Assessing The Human Health Impacts Of Nutrient-Dense Diets: Clinical Trials On Regenerative Agriculture And Protein Source, Jennifer Jensen-Cloward

All Graduate Theses and Dissertations, Fall 2023 to Present

Increasing consumer demand for sustainable, animal-friendly food options has sparked discussion about farming production practices. As more people prioritize their food choices, questions have circulated about how farming practices might impact not just the environment, but also human health. Regenerative agriculture is a farming method that has gained popularity for its potential to improve both food quality and environmental outcomes. It focuses on restoring ecosystem function, ultimately impacting the resulting nutrients in food products, and includes foods like grass-fed meat and organic produce. However, it is still unclear whether these practices make a difference in human health. This dissertation informs …

Understanding Impact Of Starter Culture Blends On The Ph Variation In Cheddar Cheese During Early Aging, Samuel J. Clarke

All Graduate Theses and Dissertations, Fall 2023 to Present

Consumer expectations govern what is acceptable when it comes to the flavor, texture, and color of the cheese. These quality attributes of cheese are all influenced by the pH of the cheese which is in turn controlled by the acid production of the starter lactic acid bacteria (SLAB) used to make cheese. Whatever the final pH of the cheese is, is what determines the quality of the cheese. Unfortunately, there are some Cheddar cheese manufacturers that have observed pH variation during the early aging of their cheeses. We hypothesize that the addition of thermophilic, fast-acid producing bacteria, like Streptococcus thermophilus …

Therapeutic Potential And Enhanced Bioactivity Of Ficus Carica (Fig) Fruit: Exploring Bioactive Compounds And Nano-Additive Synergies, Mst Sumya Yeasmin, Shamima Abdul Rahman, Waheedah Abdul Hakeem, Tarek Abedin, Mohammad Nur-E-Alam

Research outputs 2022 to 2026

The present investigation deals with the phytochemical, anticancer, antioxidant, anti-inflammatory, antidiabetic, and Anti-constipation activities of Ficus carica (FC) fruit. The phytochemical analysis of FC fruit has revealed a rich array of bioactive compounds, highlighting the fruit's complex and diverse natural chemistry together with some secondary metabolites that contribute to its high antioxidant activity which was evident from 1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and Ferric reducing power (FRAP) assays. Fruit extracts of FC demonstrated anticancer activity against MCF-7 breast cancer, U2OS osteosarcoma, HepG2 liver cancer, and Huh7it liver cancer cell lines. The anti-inflammatory activity of FC fruit is attributed to steroids …

Preserve The Harvest: Canning Goat Meat At Home, Callie Adams, Andrea Schmutz

All Current Publications

Due to its lean composition and nutritional profile similar to those of other tested meats, goat is well-suited for pressure canning. Follow USDA and Utah State University (USU) Extension altitude guidelines. This fact sheet provides guidelines for selecting high-quality meat, using proper equipment, and adhering to tested methods. Home canners can safely preserve goat meat and enjoy its benefits year-round.

Fish-Spec: Fast Identification System For Handheld Spectroscopy And Species Classification, Mitchell Sueker, Nicholas Mackinnon, Gregory Bearman, Amanda Tabb, Diane Kim, Rosalee S. Hellberg, Alireza Akhbardeh, Hamid Reza Marateb, Jianwei Qin, Moon Kim, Fartash Vasefi, Hossein Kashani Zadeh

Food Science Faculty Articles and Research

Accurate fish species identification is critical to prevent mislabeling and fraud in the seafood industry. We present a handheld multi-mode point spectroscopy system that combines fluorescence (365 and 395 nm excitation) and reflectance measurements in the visible to near-infrared (∼350–900 nm) and short-wave infrared (∼900–1700 nm) regions for rapid, non-destructive classification of fish fillets. Tissue spectra were acquired at 25 positions on 68 fillets from 11 species, in both frozen and thawed states. Feature-level fusion across all four modes enabled higher classification accuracy than any single mode alone. A global machine-learning model classified all species with 85 ± 2.8 %, …

Valorization Of Rice Bran For Functional Bioplastic Fabrication Using Plasma-Activated Water, Saydul Md Safwa, Muhammad Nazrul Islam, Keisha B. Walters, Md Mahfuzur Rahman

Food Science Faculty Publications and Presentations

While plasma has been used for decades for the surface modification of packaging films, in this study, rice bran (RB) and rice bran protein (RBP) films were developed using plasma-activated water (PAW), and the chemical composition, surface structure modifications, surface properties, and thermal properties of these films were characterized. Fourier-transform infrared spectroscopy (FTIR) of the bran and protein films showed that the reactive species in PAW improved hydrogen bonding, as evidenced by the increase in the O-H stretching band at 3300 cm-1 and reduced peak intensity at 2940-2925 cm-1 (C-H stretching), suggesting oxidation of the aliphatic side chains. For the …

The Role Of Nutrition-Sensitive Interventions In Improving Nutritional Outcomes: Findings From A Systematic Review And Meta-Analysis, Thomas De Hoop, Adria Molotsky, Rebecca Walcott, Pablo Gaitán-Rossi, Sonia Hernández-Cordero, Amos Laar, Torben Behmer, Hoa Thi Mai Nguyen, Averi Chakrabarti, Garima Siwach, Varsha Ranjit, Vania Lara-Mejía, Bianca Franco-Lares, Mireya Vilar-Compte

Department of Nutrition and Food Studies Scholarship and Creative Works

Background

Maternal and child undernutrition remains a major global health concern despite modest progress. Accelerating reductions in stunting and wasting will require increased investments in nutrition-sensitive interventions, which target nutrition impacts outside of the healthcare setting. This review examines the effects of four types of nutrition-sensitive interventions —cash/food transfers, nutrition-sensitive agriculture, water/sanitation/hygiene, and school nutrition— on maternal and child nutrition outcomes and dietary diversity.

Methods

We synthesized the evidence using an initial broad search and synthesis for nutrition-sensitive interventions, followed by targeted searches and syntheses for specific interventions and nutrition outcomes. Meta-analyses were performed to evaluate the impacts of cash …

Prediction Model Of Storage Quality Change And Shelf Life Of Dried Pork Slice, Chen Xiaohe, Qian Ping, Huang Ning, Yang Ran, Qu Lingbo, Zhao Changcheng, Li Chunxia

Food and Machinery

[Objective] To investigate the quality changes of dried pork slices under different temperature storage conditions and to predict their shelf life. [Methods] Hardness, color parameters (L* value, a* value, b* value ), and sensory quality of dried pork slices were investigated under storage at 25, 35, 45, and 55℃. Pearson correlation analysis was performed for each index, and the key index was fitted using a dynamic model. Combining the Arrhenius equation with the Q10 model, a shelf life prediction model was established. [Results] Under storage at 25, 35, 45, and 55 ℃, the a* value of dried pork slices decreased …

Analysis Of Aroma Volatile Compounds In Black Tea Produced From The Large-Leaf Variety In Northern Guangxi Based On Hs-Spme-Gc-Ms, Ting Chen, Chunyu Guo, Yingqi Wu, Min Li, Yuelan Pang, Yan Qing

Journal of Food Science and Biotechnology

[Objective ] This study explores the unique flavor characteristics and volatile compounds of black tea produced from the large-leaf variety in northern Guangxi,aiming to reveal the material basis of aroma formation of this variety.[Method ] Sensory evaluation,determination of biochemical parameters,headspace-solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS ),and odor activity value (OAV ) analysis were performed in combination with chemometrics analysis to analyze the flavor characteristics and volatile compounds of the black tea.[Result ] The black tea samples produced from the large-leaf variety in northern Guangxi had similar aroma properties and were characterized by distinct floral and fruity aromas.A total of 88 volatile …

Advances In The Discovery Of Active Natural Products By Large Fragment Cloning, Dandan Zeng, Jiayu Chen, Tianlu Mo

Journal of Food Science and Biotechnology

Active natural products have diverse structures and activities and can be a major source of small molecule drugs.Given the emergence of multi-drug resistant bacteria and superbacteria,there is an urgent need to develop new active natural products.Large fragment cloning has been an important tool for the discovery of active natural products in recent years,and it has demonstrated obvious advantages in obtaining the biosynthetic gene clusters of active natural products.This approach,combined with efficient cluster-targeted screening methods,has significantly accelerated the discovery of active natural products.This review summarizes a wide variety of active natural products.Furthermore,this paper elaborates on the principles and applications of several …

Preparation And Application Of A Polyclonal Antibody To Chlamydomonas Reinhardtii Rsp 10, Chan Lu, Yuanyuan Zhang, Ye Tao, Zhenchuan Fan, Yanxia Liu

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the cilium assembly of the radial spoke protein RSP 10 and develop a highly specific polyclonal antibody to RSP 10.[Method ] Firstly,the prokaryotic expression vector pMal-C 2X-rsp10 was constructed,and the expression of MBP-labeled RSP 10 was induced in Escherichia coli BL21 (DE3).After the fusion protein was purified,and the MBP label was excised.The obtained protein was used to immunize New Zealand male white rabbits,and the antibody titer in the anti-serum was determined by indirect ELISA.[Result ] The rabbit anti- Chlamydomonas reinhardtii RSP 10 antibody with high specificity and high sensitivity was obtained after purification …

Fabrication And Antioxidant Property Characterization Of Caffeic Acid Phenethyl Ester-Loaded Nanoparticles, Yaqiong Chen, Yumeng Li, Hui Wu, Hongdong Song, Xiao Guan

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the dispersion and utilization of caffeic acid phenethyl ester (CAPE ) in aqueous solutions.[Method ] CAPE-loaded hordein (CAPE-HD ) nanoparticles were constructed via an anti-solvent precipitation method.The physicochemical properties of the nanoparticles were characterized,and the antioxidant properties of the nanoparticles within Caco- 2 cells were investigated.[Result ] The components within the CAPE-HD nanoparticles interacted through non-covalent bonds,such as hydrogen bonding,resulting in an altered state of CAPE upon nanoparticle formation.The CAPE-HD nanoparticles exhibited near-spherical morphology with an average particle size of 193.93 nm,an average polydispersity index (PDI) of 0.26,and average Zeta potential of 14.50 …

Bacterial Diversity In Milk And Traditionally Fermented Dairy Products In Balin Right Banner, Lijuan Liu, Gaowa Bai, Jinghe Yu, Fang Wang, Youyi Zhang, Li Zhu, Dalai Wuyun

Journal of Food Science and Biotechnology

[Objective ] This study explored the bacterial diversity in milk and traditional fermented dairy products in Balin Right Banner,aiming to lay a foundation for the development and utilization of bacterial resources in the milk and traditional fermented dairy products in this region.[Method ] A total of 32 samples of cow milk,milk tofu,and sour fermentation supernatant of milk tofu were collected from Balin Right Banner,and their bacterial diversity was analyzed by 16S rRNA high-throughput sequencing.[Result ] The diversity and abundance of bacterial communities varied among the milk and traditional fermented dairy products in Balin Right Banner.The dominant bacterial phyla were Firmicutes …

Antimicrobial Resistance And Transmission Risk Of Foodborne Escherichia Coli In Wet Markets, Sisi Li, Wei Zhou, Zhijin Zhou, Bin Wang, Chengjun Qiu, Zhangsheng Zhang, Qi Zhang, Yun Sun, Xu Xu, Tao Shen, Jianzhong Han, Daofeng Qu

Journal of Food Science and Biotechnology

[Objective ] To evaluate the antimicrobial resistance and transmission risk of foodborne Escherichia coli in wet markets,this study isolated E.coli from pork,aquatic products,vegetables,and wastewater collected from three wet markets in Hangzhou,Zhejiang.[Method ] The isolates were identified by 16S rRNA sequencing,and their antimicrobial resistance phenotypes were determined via the disk diffusion method.PCR was employed to detect antimicrobial resistance genes and integrons in the isolates.The antimicrobial resistance characteristics of E.coli were analyzed,and conjugation experiments were performed to assess the potential for horizontal transfer of antimicrobial resistance genes.Co-occurrence network analysis was conducted to evaluate the transmission risk of antimicrobial resistance among the isolates.[Result …

A Zif- 8 Electrode Immobilizing Enzyme For Detection Of Glucose Solution In Food, Tingmei Ma, Shouxin Tang, Wenpan Shi, Yuchen Ma, Haijun Du

Journal of Food Science and Biotechnology

[Objective ] The aim was to construct an electrochemical sensor based on a metal-organic framework-modified electrode for the highly sensitive detection of glucose solution in beverages.[Method ] An electrochemical sensor based on ZIF- 8 composite carbon nanotubes and immobilizing glucose oxidase (GOx ) was prepared by in-situ synthesis and immobilized enzyme technology.The microstructure and morphology of the material were characterized by X-ray diffraction (XRD ),scanning electron microscopy (SEM ),UV-Vis spectroscopy,and infrared spectrometry (IR).The electrochemical performance of the electrode immobilizing enzyme was tested by electrochemical workstation.[Result ] The prepared electrode immobilizing enzyme showed excellent detection performance for glucose solution,with a limit …

Establishment Of A Molecularly Imprinted Electrochemical Sensor Nh 2-Mwcnts@ 4-Fpba For The Detection Of Epidermal Growth Factor In Dairy Products, Ruyue Zhang, Yiwei Wang, Hongxiu Zeng, Bini Wang, Fuxin Zhang, Zhenquan Huo, Yufang Liu

Journal of Food Science and Biotechnology

[Objective ] This study aims to develop a rapid and accurate method for detecting epidermal growth factor (EGF ).[Method ] 4-Formylphenylboronic acid (4-FPBA ) was covalently anchored to the surface of aminocarbon nanotubes (NH 2-MWCNTs ) to synthesize nanocomposites (NH 2-MWCNTs@ 4-FPBA ),and NH 2-MWCNTs@ 4-FPBA was modified on a glassy carbon electrode.When EGF was present,4-FPBA captured the EGF template through benzaldehyde,and then the electrode was immersed in pyrrole solution to electropolymerize to form a pyrrole molecularly imprinted layer.Finally,after dissociation and removal of the EGF template in an acidic solution,a three-dimensional cavity complementary to the EGF template was formed.[Result ] …

Comparison Of The Bioactivities Of Ziziphus Jujuba Polysaccharide And Its Degradation Products, Chunling Zhou, Xiaoyan Huang, Xuan Zheng, Ju Zhang

Journal of Food Science and Biotechnology

[Objective ] This study aims to compare Ziziphus jujuba polysaccharide (ZJP) and its degradation products in terms of their chemical composition and bioactivities.[Method ] ZJP was degraded by α-L-rhamnosidase,cellulase,snail enzyme,and trifluoroacetic acid to prepare different degradation products (ZJP-R,ZJP-C,ZJP-S,and ZJP-T ),and the chemical composition and degradation effects were compared.On the basis of in vitro antioxidant and probiotic activities,the in vivo antioxidant,anti-aging,anti-stress,and neuroprotective activities of ZJP and its degradation products were compared with Caenorhabditis elegans as a model system.[Result ] The mass fraction of reducing sugar and average degree of polymerization of ZJP showed significant variations among different degradation treatments.In addition,Sephadex G- …

Immunomodulatory Effects And Marker Components Of Alcohol Extract From Acanthopanax Senticosus (Rupr . Et Maxim .) Harms- Ganoderma Applanatum (Pers.) Pat., Lihong Wang, Kairui Sheng, Ming Lu, Jin Su, Yang Ping, Yu Zhang, Ji Zhao, Yixia Gong

Journal of Food Science and Biotechnology

[Objective ] The aim of this study was to analyze the compositional changes of the alcohol extracts of Acanthopanax senticosus (Rupr.et Maxim.) Harms (APS )-Ganoderma applanatum (Pers.) Pat.(GA) before and after compounding at a mass ratio of 5∶1 (mAPS∶mGA=5∶1) and to investigate the immunomodulatory effects and marker components of the extracts.[Method ] The mouse models of compromised immunity and delayed-type hypersensitivity (DTH ) were established with cytoxan (CTX ) and 2,4-dinitrochlorobenzene (DNCB ),respectively,to investigate the effects of the alcohol extracts on the immune system.Ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS ) was used to analyze the changes of the active ingredients …

Effects And Differences Of (+)-Catechin And (-)-Epicatechin On Antioxidant Activity Of Hek 293t Cells, Chang Sun, Ning Xia, Chao Wang, Fengxian Qin, Chengyu Lyu, Jian Liu, Tiezhu Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects and differences of the natural antioxidant (+)-catechin and its isomer (-)-epicatechin on the antioxidant activity of HEK 293T cells based on the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE ) pathway.[Method ] The effects of (+)-catechin and (-)-epicatechin on the viability of HEK 293T cells were investigated via the methyl thiazolyl tetrazolium (MTT ) assay.Reporter genes were employed to investigate the regulatory effects on the activation of ARE.The effects of (+)-catechin and (-)-epicatechin on the mRNA and proteins levels of the downstream factors [glutamate-cysteine ligase modifier subunit (GCLM …

Effects Of Α-Amylase Addition On The Characteristics Of Infant Rice Flour, Huihui Liu, Zhiyong Dai, Mengyi Li, Jiao Peng, Xing Meng, Luyan Liao, Rongbin Zhang

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of α-amylase addition on the characteristics of infant rice flour.[Method ] Infant rice flour samples with different α-amylase addition were prepared.The basic composition,relative molecular mass of starch,gelatinization properties,hydration properties,texture properties,in vitro digestion characteristics,and microstructure of infant rice flour were determined.Furthermore,the correlations between the physicochemical characteristics and quality characteristics of infant rice flour were analyzed.[Result ] The weight-averaged molecular weight and the number-averaged molecular weight of starch in infant rice flour decreased with the increase in α-amylase addition.The gelatinization curve of infant rice flour with α-amylase addition was relatively gentle,and the gelatinization …

Effects Of Spices On Microbial Community Structure And Volatile Flavor Components Of Fuling Pickled Mustard Tuber, Ting Ren, Yuling Liu, Zhiping Zhao, Xiaoyong Chen, Yumei Peng, Hong Tao, Yuanli Luo

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of spices on the microbial community structure and volatile flavor compounds of Fuling pickled mustard tuber and explore the correlations between dominant microbial genera and volatile components.[Method ] The effects of spices on microbial community structure and volatile flavor components of Fuling pickled mustard tuber were investigated by high-throughput sequencing and headspace solid-phase microextraction (HS-SPME ) coupled with gas chromatography-mass spectrometry (GC-MS ).Pearson correlation coefficients were calculated to reveal the correlations between dominant microbial genera and volatile components.The mass fractions of total acids,nitrite,and reducing sugar were determined.[Result ] The dominant microbial …

Effects Of Shaping And Baking Processes On The Antioxidant Activity Of Round Roasted Green Tea, Jun Liu, Gong Feng, Rong Tan, Weihong Huang

Journal of Food Science and Biotechnology

[Objective ] This study investigated the effects of shaping and baking processes on the antioxidant activity of round roasted green tea,aiming to provide theoretical support and practical reference for improving the quality and optimizing the processing techniques of green tea.[Method ] The influences of different processing stages and key procedures such as shaping and baking on the antioxidant activity of round roasted green tea were studied.A zebrafish model (in vivo ) and multiple indexes including DPPH radical scavenging rate,ABTS ⁺ radical scavenging rate,and ferric ion reducing antioxidant power (FRAP ) were employed for comprehensive evaluation of the antioxidant activity.[Result ] …

Effects And Mechanisms Of Phytosterol-Oil Bodies Emulsion On Lipid Metabolism Of Caenorhabditis Elegans, Jie Sun, Mengyuan Yuan, Yifei Liu, Fuyao Zhang, Zhuofan Yang, Guoyou Yin

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the lipid-lowering effects of the phytosterol-oil bodies emulsion (POE ).[Method ] Caenorhabditis elegans was taken as a model to evaluate the effects of POE on lipid metabolism,physiological indexes,and expression of genes associated with oxidative stress and fat metabolism.[Result ] Compared with the control group,different mass concentrations of POE reduced the mass fraction of fat (P<0.01) and the relative content of triglycerides (P<0.05),without toxicity to the growth and development of C.elegans (P<0.01).At the same time,it significantly reduced the level of reactive oxygen species and increased the levels of antioxidant enzymes.In addition,POE affected the expression levels of genes related to fat metabolism.qPCR results showed that POE up-regulated the mRNA level of sbp-1,while down-regulating the mRNA levels of fat-2,nhr-80,and fat-5 (P<0.05).[Conclusion ] POE may improve lipid metabolism by slowing fat deposition,regulating the fat peroxidation pathway,and alleviating oxidative stress in C.elegans.

Optimization Of Processing Technology And Analysis Of Volatile Components In Jinxuan Black Tea Cream, Yunxian Wang, Yunxiong Zhao, Lianfeng Luo, Baogui Liu, Jingjing Yang

Journal of Food Science and Biotechnology

[Objective ] This study is intended to optimize the preparation process of Jinxuan black tea cream,enhance the content of tea polyphenols,and compare the effects of different processing methods on the volatile components of the tea cream.[Method ] With dried Jinxuan black tea as the raw material,response surface methodology was adopted to examine the impacts of tea-to-water ratio,enzyme dosage,ultrasonic power,and ultrasonic duration on the mass fraction of tea polyphenols in the black tea cream.Furthermore,headspace solid-phase microextraction (HS-SPME ) coupled with gas chromatography-mass spectrometry (GC-MS ) was employed to detect and analyze the volatile components in the black tea cream samples processed …