Food Science Commons^™

Landscape And Garden Plants Sample Submission To The Plant And Pest Diagnostic Clinic, Kevin Korus, Tamra Jackson-Ziems, James A. Kalisch, Lowell Sandell, Terri James

Nebraska Cooperative Extension: Faculty and Staff Publications

Diagnoses are made easier and turnaround time is improved when the quality of plant samples is maintained. This NebGuide discusses the proper guidelines for submitting landscape and garden plants, turf, and tree samples to UNL’s Plant and Pest Diagnostic Clinic.

There are several important things to consider when collecting, packaging, and sending plant or insect samples for diagnosis in a plant diagnostic laboratory. Collect an adequate amount of plant material. When feasible, the sample should include the entire plant — roots, stems, leaves, flowers, and fruits. Whole plants are needed to properly evaluate the sample. If the whole plant is …

Planting The Landscape, Kim Todd, Elizabeth Killinger, Terri James

Nebraska Cooperative Extension: Faculty and Staff Publications

A landscape is an investment of time and money that can be a beautiful, functional, and satisfying living space. Gardeners and landscape managers implementing integrated pest management strategies through good judgment in plant selection, site preparation, and planting practices will reduce insect, disease, and wildlife damage and will help ensure the growth and longevity of the investment.

Proper plant selection, site preparation, and planting practices are aspects of integrated pest management. Continued integration of correct cultural practices is important through proper mulching, watering, fertilizing, and other management practices to reduce pests.

Effects Of Acute And Chronic Sleep Deprivation On Eating Behavior, Stephanie Frank

Lewis Honors College Capstone Collection

Sleep-deprivation is thought to be a factor in the rising American obesity epidemic. Altered sleep cycles affect appetite-regulating hormone levels and enhance the hedonic effect of food consumption. As a result, sleep restriction has been associated with an increased daily energy intake of more than 500 calories and high-carbohydrate/high-fat consumption. However, past studies have not examined the relationship between sleep deprivation and decreased intake of nutrient-dense fruits and vegetables as a factor in increased energy consumption. This paper aims to 1) determine the affect average hours of sleep or acute sleep deprivation (defined as less than 6 hour sleep per …

The Hang-Up With Hamburg: How Center For Food Safety V. Hamburg Will Alter The Food Industry, Joella Roland

Journal of Business & Technology Law

No abstract provided.

Investigation Of Pyrrolizidine Alkaloids In Foods Using Liquid Chromatography Mass Spectrometry, Caroline Griffin

PhDs

Pyrrolizidine Alkaloids (PAs) are known plant toxins which can cause hepatic veno-occlusive disease (HVOD) in both humans and livestock when ingested. Although PAs are primarily known for their hepatotoxicity they also induce genotoxicity, carcinogenicity and pneumotoxicity. PAs are consumed through plants as food, for medicinal purposes, or as contaminants of agricultural crops. Aside from ingesting the plants directly PA exposure may occur through the consumption of honey produced by bees that visit PA-containing plants or by drinking milk produced by animals that have consumed PA-containing plants. Possible PA contamination in our food chain is a potential health risk.

To assess …

The Role Of Underutilized Fish In New England's Seafood System, Taylor Witkin

Honors Theses

The global fisheries crisis has led to an increasing recognition for the need to relieve pressure on overfished, popular food fish stocks. Opportunities exist to shift consumer demand toward more sustainable choices, including fish that may be locally abundant. Attempts have been made to market underused fish (sometimes termed “trash fish”) that represent more sustainable alternatives; however, it is unclear whether consumers will choose to purchase these more sustainable options, particularly if underused fish are also unfamiliar. Chapter 1 reviews existing research and current issues surrounding sustainable seafood and the recent shift toward local, abundant, undervalued species in New England’s …

Isolation And Characterization Of Natural Antimicrobials From Plant And Marine Organisms, Corene Barbara Canning

Wayne State University Dissertations

Antibiotic agents have been found to have a limited lifespan due to the ability of microrganisms to develop antimicrobial resistance. In this study a number extracts of plant and marine organisms were evaluated for their antimicrobial activity against a number of high priority pathogens. Bioassay-guided fractionation of four extracts led to the identification of compounds with inhibitory activity; including one previously unknown compound. Compounds were evaluated through a variety of techniques including broth microdilution assay to determine each compound's MIC and MBC. The majority of the compounds showed antimicrobial activity equal to, if not better than, the commonly used antimicrobial …

Characterization Of Bacteriophages From Environmental Water Samples And The Potential Of Bacteriophages Tailspike Proteins (Tsp) In Bacteria Detection, Gayathri Upeksha Gunathilaka

Wayne State University Theses

ABSTRACT

CHARACTERIZATION OF BACTERIOPHAGES FROM ENVIRONMENTAL WATER SAMPLES AND THE POTENTIAL OF BACTERIOPHAGES TAILSPIKE PROTEINS (TSP) IN BACTERIA DETECTION

by

GAYATHRI UPEKSHA GUNATHILAKA

May 2014

Advisor: Dr. Yifan Zhang

Major: Nutrition and Food Science

Degree: Master of Science

The high abundance of phage in the environment and their specificity with the bacteria host make them an excellent tool for bacteria detection. To characterize phage specific to Salmonella and E. coli from water samples in the Southeastern Michigan and to develop a novel bacterial detection method using phage, environmental water samples were collected from rivers and waste water treatment plant (WWTP) …

Herbal And Microbial Products For The Management Of Obesity, Maha Salama, Soheir El Zalabani, Essam Abdel Sattar

Pharmacy

Obesity is a global epidemic and one of the major health burdens of modern times. The prevalence of obesity is increasing worldwide; it constitutes a serious problem in developed as well as developing countries. Beside adults, the number of obese teenagers and children in particular has dramatically increased. Obesity is characterized by accumulation of excess fat in adipose tissues in an extent to produce adverse effects on health, leading to a reduction in life expectancy and/or a raise in health hazards. People are classified as overweight (pre-obese) and obese on the basis of the Body Mass Index (BMI), crude measure …

Knockanore Irish Farmhouse Cheese, Eamonn Lonergan

Pamphlets

No abstract provided.

Food Island, The Irish Times, A Special Report, The Irish Times

Pamphlets

No abstract provided.

Introduction: Tickling The Palate. Gastronomy In Irish Literature And Culture, Eamon Maher, Máirtín Mac Con Iomaire

Books/Chapters

There has been a gradual but noticeable growth in scholarship concerning food globally, particularly in the last decade. One of the longest running and most inf luential forces behind this phenomenon is the Oxford Symposium on Food and Cookery (1981–present) which was originally founded and co-chaired by Alan Davidson, pre-eminent food historian, diplomat, and author of The Oxford Companion to Food, and Dr Theodore Zeldin, the celebrated social historian of France. This spawned a dedicated publishing house, Prospect Books, which published the conference proceedings and also the journal Petits Propos Culinaires (PPC), now approaching its 100th issue.

Modelling The Effects Of Natural Antimicrobials As Food Preservatives, Amit Jaiswal, Swarna Jaiswal

Books/Book Chapters/ Proceedings

A predictive food microbiological model is a mathematical representation of mechanisms that describes the growth, survival, inactivation or biochemical process of an organism and are used in the food industry to predict food pathogen growth and to help in the evaluation of food safety. Furthermore, it reduces the number of expensive and time-consuming experiments; however, adequate statistical analysis is a crucial step during all phases of model development and validation. Therefore, the present chapter is focused on the basic concept of mathematical modelling which can be applied to estimate the effects of natural antimicrobials as food preservatives. Initial section discusses …

Screening Of Natural Antimicrobial Agents And Antimicrobial-Resistant Bacteria Using A Soleris System, Mingyang Huang

Wayne State University Theses

ABSTRACT

SCREENING OF NATURAL ANTIMICROBIAL AGENTS AND ANTIMICROBIAL - RESISTANT BACTERIA USING A SOLERIS SYSTEM

by

MINGYANG HUANG

May 2014

Advisor: Dr. Yifan Zhang

Major: Food Science and Nutrition

Degree: Master of Science

Traditional broth micro-dilution method is a common assay of measuring Minimum Inhibitory Concentration (MIC) to determine the antimicrobial activity of an antimicrobial agent. However, this method is generally time-consuming and labor intensive. Alternatively, an automated optical method using the Soleris system was applied in this study. The system was compared to the traditional broth micro-dilution 96-well assay to test the antimicrobial activity of 11 essential oils and …

Antimicrobial Activity Of Essential Oil Emulsions And Possible Synergistic Effect On Food Borne Pathogens, Varun Tahlan

Wayne State University Theses

The objective of this study was to evaluate the antimicrobial activity of essential oil emulsions against food borne pathogenic bacteria and determine potential applications. The oils used for this study were cinnamon, oregano, clove, thyme, rosemary, sage, bergamot, nutmeg, lemon grass and bay. Oil in water emulsions were prepared using Tween 80 as an emulsifying agent, with a stock oil concentration in the emulsions of 20,000 ppm. Essential oil emulsions were individually screened against E. coli (ATCC 25922), E. coli (ATCC 700927), L. monocytogens (ATCC 19115), L. innocua (ATCC 33090) and S. Typhimurium (ATCC 19585) using the broth micro dilution …

Bacteriophage Isolated From The Environment And E. Coli Of Meat Origin As A Reservoir Of Antibiotic Resistance, Manisha Polur

Wayne State University Theses

ABSTRACT

BACTERIOPHAGE ISOLATED FROM THE ENVIRONMENT AND E. COLI OF MEAT ORIGIN AS A RESERVOIR OF ANTIBIOTIC RESISTANCE

by

MANISHA POLUR

April 2014

Advisor: Dr. Yifan Zhang

Major: Nutrition and Food Science

Degree: Master of Science

This study was aimed to determine the extent of antibiotic resistance reservoir in bacteriophage. Bacteriophages were isolated and purified from waste water treatment plants, Detroit river, Belle Isle, Urban Garden soils and examined for the presence of beta-lactam antibiotic resistance genes. Thirty E. coli isolates from retail meat were analyzed for the presence of beta-lactam antibiotic resistance genes. 95% of E. coli them carried …

College Students’ Perceptions Regarding Sensory Aspects Of Conventionally Produced And Unconventionally Produced Foods: Implications For Marketing To The Millennial Generation, Christina Crowder, Catherine W. Shoulders, K. Jill Rucker

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Consumers vote every day on which products line the shelves of grocery stores, co-ops, and niche markets. Public unrest with regard to the environmental, animal welfare, food purity, and human health impacts of agricultural production practices have led to the rise of unconventionally produced (UP) food products. While the sales of UP foods is increasing, studies regarding the qualities of such products that impact consumer purchases have yielded inconsistent results. This study examined students’ perceptions of sensory aspects of conventionally produced (CP) and UP foods to better understand how sensory aspects impact decisions to purchase. Students reported consistent perceptions regarding …

การต้านจุลินทรีย์และการลดเอทิลีนของฟิล์มไคโตซานที่เติมไทเทเนียมไดออกไซด์, ปฏิญญา แก้วกลิ่น

Chulalongkorn University Theses and Dissertations (Chula ETD)

งานวิจัยนี้ศึกษาผลของการเติมไทเทเนียมไดออกไซด์ (TiO2) ต่อสมบัติของฟิล์มไคโตซาน เพื่อการนำไปใช้ประโยชน์เป็นบรรจุภัณฑ์อาหาร โดยเตรียมฟิล์มไคโตซานที่เติม TiO2 ความเข้มข้นแตกต่างกัน เป็น 0 0.25 0.5 1 และ 2%w/w ไคโตซาน โดยให้สัญลักษณ์เป็น C-T0 C-T0.25 C-T0.5 C-T1 และ C-T2 ตามลำดับ ศึกษาสมบัติทางกายภาพของฟิล์มที่เตรียมได้ ได้แก่ tensile strength (TS) elongation at break (EAB) สัมประสิทธิ์การซึมผ่านของไอน้ำ(WVP) และการส่องผ่านของแสง ศึกษาโครงสร้างของฟิล์มที่เตรียมได้โดยใช้เครื่อง X–Ray Diffractometer (XRD) พันธะทางเคมี โดยใช้เครื่อง Fourier Transform Infrared Spectrometer (FT-IR) และลักษณะพื้นผิวและภาคตัดขวางของฟิล์มไคโตซาน วิเคราะห์โดยใช้กล้องจุลทรรศน์อิเล็กตรอนแบบส่องกราด (Scanning Electron Microscope, SEM) ศึกษาสมบัติการยับยั้งการเจริญของแบคทีเรีย ได้แก่ Escherichia coli Pseudomonas aeruginosa Salmonella Typhimurium และ Staphylococcus aureus และเชื้อรา ได้แก่ Aspergillus oryzae และ Penicillium roqueforti รวมทั้งศึกษาการกำจัดก๊าซเอทิลีน จากผลการทดลองพบว่าค่า TS เพิ่มขึ้นเมื่อความเข้มข้น TiO2 เพิ่มขึ้นจนถึง 1% แล้วจึงลดลง ในขณะที่ค่า EAB และ WVP ลดลงเมื่อเติม TiO2 แต่การเติม TiO2 ที่ความเข้มข้นแตกต่างกันไม่ส่งผลให้ค่าดังกล่าวมีความแตกต่างกันอย่างมีนัยสำคัญ (p < 0.05) ส่วนค่าการส่องผ่านของแสงมีแนวโน้มลดลงเมื่อ TiO2 เพิ่มขึ้น จากการศึกษาโครงสร้างของฟิล์มไคโตซานที่เติม TiO2 ทุกความเข้มข้นพบว่า ปริมาณผลึกอะนาเทสของ TiO2 เพิ่มขึ้นเมื่อความเข้มข้น TiO2 เพิ่มขึ้น และพบผลึกรูไทล์ในฟิล์ม C-T1เท่านั้น นอกจากนี้ยังพบไทเทเนียมออกไซด์(Ti3O5) และไฮโดรเจนไทเทเนียมออกไซด์ (H2Ti3O7) ที่ฟิล์ม C-T1และ C-T2 เมื่อศึกษาพันธะทางเคมีของฟิล์มไคโตซานที่เติม TiO2 พบว่าไทเทเนียมจะสร้างพันธะไฮโดรเจนกับไคโตซานที่หมู่ –OH และ –NH ของไคโตซาน โดยพีคที่แสดงถึงพันธะ C–N และ C–N–H ของไคโตซานเพิ่มขึ้นเมื่อความเข้มข้นของ TiO2 เพิ่มขึ้นและพบว่าฟิล์ม C-T1 และ C-T2 มีพีคที่แสดงถึงพันธะ Ti-O-Ti สูงกว่าฟิล์มไคโตซานที่เติม TiO2 ที่ความเข้มข้นอื่นๆอย่างชัดเจน และเมื่อศึกษาลักษณะพื้นผิวและภาคตัดขวางด้วย SEM พบว่า ฟิล์มไคโตซานที่เติม TiO2 มีลักษณะพื้นผิวเรียบเนียน และมีอนุภาค TiO2 กระจายอยู่สม่ำเสมอทั่วฟิล์ม แต่เมื่อเพิ่มความเข้ม TiO2 เป็น 2% พบว่าเกิดการจับกันเองของ TiO2 จากผลการทดลองพบว่าฟิล์มที่ได้มีคุณสมบัติทางกายภาพดีที่สุดเมื่อเติม TiO2 1% จึงใช้ฟิล์ม C-T1 สำหรับการทดลองขั้นต่อไป เมื่อศึกษาผลการยับยั้งเชื้อจุลินทรีย์ของฟิล์ม C-T1 ภายใต้แสง black-light เป็นเวลา 6 ชั่วโมงพบว่า จำนวนเซลล์มีชีวิตของ E. Coli ลดลง 4.01 log CFU/mL จากจำนวนเชื้อเริ่มต้น 7.77 log CFU/mL ในขณะที่ฟิล์ม C-T0 สามารถลดจำนวนเซลล์มีชีวิตได้ 2.53 log CFU/mL และฟิล์ม C-T1 ทำให้จำนวนเซลล์มีชีวิตของ S. Typhimurium ลดลง 1.48 log CFU/mL ในขณะที่ฟิล์ม C-T0 สามารถลดจำนวนเซลล์มีชีวิตได้ 1.07 log CFU/mL อย่างไรก็ตามพบว่าฟิล์ม C-T0 สามารถยับยั้งการเจริญของ P. aeruginosa และ S. aureus ได้ดีกว่าฟิล์ม C-T1 นอกจากนี้ยังพบว่าทั้งฟิล์ม C-T0 และ C-T1 สามารถลดจำนวนเชื้อราได้เพียงเล็กน้อยและไม่มีความแตกต่างอย่างมีนัยสำคัญ จากการศึกษาการกำจัดก๊าซเอทิลีนของ C-T1 เปรียบเทียบกับฟิล์ม C-T0 และผง TiO2 พบว่าที่สภาวะมีแสง UV ฟิล์ม C-T1 สามารถสลายเอทิลีนที่ความเข้มข้นเริ่มต้น 5 ppm ได้ร้อยละ 12.58 เมื่อเวลาผ่านไป 3 ชั่วโมง โดยค่าที่ได้แตกต่างอย่างมีนัยสำคัญ (p < 0.05) จากค่าร้อยละการสลายเอทิลีนของผง TiO2 ซึ่งมีค่าเท่ากับ 14.13 ในขณะที่ฟิล์ม C-T0 แสดงการลดลงของเอทิลีนเพียงร้อยละ 3.58

Combined Xenon Hydrate Formation And Freezing For Preservation Of Plant And Fruit Tissues, Thunyaboon Arunyanart

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aimed to study a combined method of xenon hydrate formation and freezing (CXF) for preservation of plant and fruit tissues, and to compare CXF with the freezing alone process (FAP). The use of nuclear magnetic resonance (NMR) technique based on proton spin-spin relaxation time (T2) of water to indicate freezing damage in plant and fruit tissues was also proposed. Due to its simple tissue composition, barley coleoptile tissue was used in this study so that xenon hydrate formation can be easily observed. Apple parenchyma tissue was used to represent edible fruit tissue. CXF includes two steps. The first …

Valorisation Of The Cider And Brewing Industry By-Products As Nutraceutical Ingredients, Sofia Reis

Doctoral

By-products from the cider and brewing industries, including apple pomace (AP) and brewer’s spent grain (BSG) respectively, constitute major environmental problems due to the large quantities produced every year. They are sources of valuable compounds such as protein, fibre, essential fatty acids, phenolic compounds and minerals, some of them with antioxidant and prebiotic properties. Their high nutritional value leads to their potential use as human food products and several applications have been considered recently. The aims of this work include i) the nutritional and compositional characterisation of AP, BSG and final extruded and baked prototypes containing these by-products and ii) …

Effectiveness Of A Farm Field Trip, Bonnie S. Sigmon

Theses and Dissertations--Community & Leadership Development

The annual Sigmon Farm Tour was started in 1992 as an agricultural education program where students could experience being on a farm with the goal of increasing the agricultural literacy levels of the participants. Every year the entire 4th grade student population of Rockcastle County spends the day touring the farm and participating in experiential mini lessons given by the cooperating farm service and health agencies. The program has continued for 20+ years without an evaluation as to whether it is achieving its objectives. This evaluation will also exhibit the programs strengths and weakness so it can continue to improve. …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Ultrasound Assisted Hydration Of Navy Beans (Phaseolus Vulgaris), N. Misra

Articles

The use of ultrasound to enhance the transport phenomena in food processes has been well recognised in recent times. The objective of this study was to evaluate the effect of sonication on hydration rate and pasting profile of navy beans. The hydration kinetics for control and ultrasound assisted soaking was mathematically described using mechanistic (Fickian diffusion) and empirical (Peleg’s equation, Weibull model and First Order equation) models. Ultrasound enhanced the rate of hydration which was evident from the plot of kinetic data and model parameters. The effective diffusivities for water transport without and with ultrasound application were estimated to be …

Apple Pomace As A Potential Ingredient For The Development Of New Functional Foods, Sofia Reis, Dilip K. Rai, Nissreen Abu-Ghannam

Articles

Extruded snacks and baked scones were formulated with increasing levels (0-30%) of apple pomace (AP). The incorporation of up to 20% of AP in extruded snacks and in baked scones does not change significantly (pβ-carotene/linoleic acid system) increased when compared to the products to which no AP was added. Chlorogenic acid and quercetin were the major phenolic compounds found in the products. Loss of phenolic compounds during heat treatment occurred, however the DPPH radical scavenging activity of final products was not affected.

Atmospheric Cold Plasma Inactivation Of Escherichia Coli, Salmonella Enterica Serovar Typhimurium And Listeria Monocytogenes Inoculated On Fresh Produce, Dana Ziuzina, Sonal Patil, Patrick J. Cullen, Kevin Keener, Paula Bourke

Articles

Atmospheric cold plasma (ACP) represents a potential alternative to traditional methods for non-thermal decontamination of foods. In this study, the antimicrobial efficacy of a novel dielectric barrier discharge ACP device against Escherichia coli, Salmonella enterica Typhimurium and Listeria monocytogenes inoculated on cherry tomatoes and strawberries, was examined. Bacteria were spot inoculated on the produce surface, air dried and sealed inside a rigid polypropylene container. Samples were indirectly exposed (i.e. placed outside plasma discharge) to a high voltage (70kVRMS) air ACP and subsequently stored at room temperature for 24 h. ACP treatment for 10, 60 and 120 s resulted in reduction …

Zein Film: Effects Of Dielectric Barrier Discharge Atmospheric Cold Plasma, Shashi Pankaj, Carmen Bueno-Ferrer, N. Misra, Paula Bourke, Patrick J. Cullen

Articles

Dielectric barrier discharge atmospheric plasma is a novel non-thermal technology for the food and packaging industry. The effects of dielectric barrier discharge plasma on the surface, structural, thermal and moisture sorption properties of edible zein films have been examined. Plasma treatment increased the surface roughness and equillibrium moisture content of the zein film in a direct relationship with the applied voltage level. No significant difference in the thermal stability of the zein film is also observed after plasma treatment. Dielectric barrier discharge plasma treatments of zein film lead to a change in the protein conformation which is confirmed by X-ray …

Improved Efficiency Of Brewer’S Spent Grain Arabinoxylans By Ultrasound-Assisted Extraction, Sofia Reis, Elisabete Coelho, Manuel Coimbra, Nissreen Abu-Ghannam

Articles

Arabinoxylan (AX) rich extracts from brewer’s spent grain (BSG) were produced by the application of ultrasound-assisted extraction (UAE) and conventional alkaline extraction (AKE). UAE and AKE were optimised for the production of the highest yield of ethanol insoluble material using response surface methodology (RSM). The efficiency of UAE was established by the significant reduction of time (7h to 25 min) and energy when compared to AKE, to recover similar amount of AX (60%) from BSG, leading to the production of starch-free AX-rich extracts.

Rheological, Foam, And Physical Properties Of Low Sucrose Meringue And Angel Food Cake Formulated With Non-Nutritive Sweeteners And Polydextrose, Kevin J. O'Niones

Theses and Dissertations--Animal and Food Sciences

The object of this research was to determine if an acceptable angel food cake alternative could be produced that had reduced calories and sucrose content. This was accomplished through replacing sucrose in meringue, angel food cake batter, and baked angel food cakes with polydextrose and either sucralose, acesulfame-K, or Rebaudioside A at different replacement levels (25, 50, 75, 100%). Meringue and cake batter properties were measured using rheological techniques. Baked angel food cakes were analyzed based on height, weight loss, moisture content, color, and TPA analysis. With meringue batter, 100% sucrose replacement was unacceptable since undissolved polydextrose made analyzing and …

The Acoustic Emissions Produced By Escherichia Coli During The Growth Cycle, Traci Jane Cox

Theses and Dissertations--Animal and Food Sciences

The objective of this study was to determine if acoustic emissions (AE) generated by three strains of Escherichia Coli (5024-parent strain, 8279-mutant strain and 8279-random/unrelated strain) could be used to differentiate each strain during their growth cycle. An acoustic sensor with an operating range of 35 kHz-100 kHz was inserted into the growth vessel and attached to a selected channel to capture AE data. The growth vessel was loaded with 60 ml of tryptic soy broth (TSB) (0.25% fructose) media with alginate (1.1%) or without alginate and inoculated with 1% (10⁸ CFU/ml) of an E. coli strain. The growth …

Microbial Successions Are Associated With Changes In Chemical Profiles Of A Model Refrigerated Fresh Pork Sausage During An 80-Day Shelf Life Study, Andrew K. Benson, Jalrus R.D. David, Stefanie Evans Gilbreth, Gordon Smith, Joseph Nietfeldt, Ryan Legge, Jaehyoung Kim, Rohita Sinha, Christopher E. Duncan, Junjie Ma, Indarpal Singh

Department of Food Science and Technology: Faculty Publications

Fresh pork sausage is produced without a microbial kill step and therefore chilled or frozen to control microbial growth. In this report, the microbiota in a chilled fresh pork sausage model produced with or without an antimicrobial combination of sodium lactate and sodium diacetate was studied using a combination of traditional microbiological methods and deep pyrosequencing of 16S rRNA gene amplicons. In the untreated system, microbial populations rose from 10² to 10⁶ CFU/g within 15 days of storage at 4°C, peaking at nearly 10⁸ CFU/g by day 30. Pyrosequencing revealed a complex community at day 0, with …