Food Science Commons^™

Antibacterial Effects Of Proteases On Different Strains Of Escherichia Coli And Listeria Monocytogenes, Hanan Eshamah

All Dissertations

Escherichia coli O157:H7 and Listeria monocytogenes are pathogens that have received special attention by federal agencies, food safety researchers and food industries due to their economic and human health impact. To reduce the presence of these pathogens, alternative interventions have been studied. However, increasing consumer's demand for natural ingredients has made the investigations of effectiveness of natural antimicrobials necessary. In this study, in vitro antimicrobial activity of bromelain and papain against E. coli JM109 and L. monocytogenes was investigated. Furthermore, actinidin and papain were evaluated to reduce populations of L. monocytogenes strain and three mixed strains of E. coli O157:H7 …

An Evaluation Of The Nontarget Effects Of Transgenic Bacillus Thuringiensis Maize On Arbuscular Mycorrhizal Fungi In The Soil Ecosystem, Tanya Elizabeth Amy Cheeke

Dissertations and Theses

My dissertation research examined the effect of the cultivation of insect-resistant Bacillus thuringiensis (Bt) maize on the soil environment with a goal of understanding how to obtain a balance between technological advancement and maintenance of a healthy soil ecosystem. Although Bt plants may help to reduce pesticide use, conferring benefits to farm workers and the environment, there are still unresolved questions about how the cultivation of Bt plants affects soil organisms. For this dissertation project, I used 14 different genotypes of Bt maize and non-Bt maize (Zea mays) to investigate the effects of transgenic Bt plants on the colonization ability, …

Mutational Analysis Of Sse1 (Hsp110) Suggests An Integral Role For This Chaperone In Yeast Prion Propagation In Vivo, Ciara Moran, Gemma Kinsella, Zai-Rong Zhang, Sarah Perrett, Gary Jones

Articles

ABSTRACT The yeast Hsp110 chaperone Sse1 is a conserved protein that is a noncanonical member of the Hsp70 protein superfamily. Sse1 influences the cellular response to heat stress and has also been implicated in playing a role in the propagation of prions in yeast. Sse1 can seemingly exert its effects in vivo through direct or indirect actions by influencing the nucleotide exchange activity of canonical cytosolic Hsp70s. Using a genetic screen based on the inability to propagate the yeast [PSI+] prion, we have identified 13 new Sse1 mutants that are predicted to alter chaperone function through a variety of different …

Development Of Rapid Detection Methods And Novel Control Measures For Salmonella In Poultry, Si Hong Park

Graduate Theses and Dissertations

This dissertation consists of three research parts: 1) development of rapid detection methods for foodborne pathogens; 2) immune response of chicken cells against Salmonella and bacteriophage P22; 3) evaluation of novel control measures for poultry productions. In order to develop rapid and accurate detection methods for foodborne pathogens, two types of PCR assays were utilized. Three foodborne pathogens included Campylobacter, Escherichia coli and Salmonella in watershed were qualitatively and quantitatively detected by multiplex PCR and qPCR (chapter 2). Since Salmonella species are commonly present in poultry and poultry products as well as most popular foodborne pathogen in …

The View From The Table: An Analysis Of Participant Reactions To Community-Based Dialogues On Food And Justice, Jennifer Turner

Dissertations and Theses

While Portland, Oregon's sustainable food movement wins accolades for explicitly situating itself in opposition to the industrialized global food system, it often fails to address systems of oppression that are reproduced within the alternative agri-food movement itself. This demonstrated aversion towards the messy, complex, contingent nature of the social world reflects larger processes of "de-politicization" of the overall sustainability agenda, which leads to the favoring of technological and/or spatial solutions that may undermine the social equity and justice dimensions of the "triple bottom line." This thesis focuses on an action research project involving a series of community dialogues that provided …

Fearless: Heather Thomas, Heather L. Thomas

SURGE

This week we would like to recognize recent graduate and AmeriCorps VISTA volunteer Heather Thomas ’13, who is returning to Gettysburg as the fearless new coordinator for the Adams County Food Policy Council. [excerpt]

Milking The System: Do Poor People Deserve Fresh Food?, Melanie M. Meisenheimer

SURGE

Poor Americans are all lazy, selfish people who must first prove their worth as human beings if they want to be able to feed their children.

It sounds harsh, stereotypical, and judgmental when you put it like that, and few people would feel comfortable saying that exact phrase. However, it’s a perception of poverty in America that I’ve found still has a strong grip on our way of thinking. [excerpt]

Storage Technique Preserves Citrus, Kills Pathogens, Gayle Volk, Jan Suszkiw

Agricultural Research Magazine

Americans consume about 84 pounds of fresh and processed citrus per person each year, with oranges topping the list at 61 pounds annually. The availability of these favored fruits in grocery stores, fresh markets, and other consumer outlets is a testament to growers’ success in managing the ever-present threat of pests and diseases to the nation’s $3.4 billion citrus crop (2011-12), most of which is produced in Florida and California.

A major threat is citrus greening disease, also known as “Huanglongbing.” First detected in Florida in August 2005, citrus greening today is the target of a multifaceted effort by federal, …

Detecting Deadly Colonies Of E. Coli, Rosalie Marion Bliss

Agricultural Research Magazine

Shiga-toxin-producing Escherichia coli (STEC) serogroup O157:H7 has long been associated with severe forms of foodborne illnesses. But these bacterial bad guys have many cousins, and six other STEC groups have also been linked to serious illnesses and outbreaks of disease.

In 2011, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced a regulation that, as of June 2012, certain beef products that test positive for any of these six groups, nicknamed the “Big Six,” would be banned from being sold for public consumption. As USDA’s regulatory arm, FSIS employs inspectors who collect meat samples on the front …

Current Challenges Can Help Fuel Future Opportunities, Jeffrey J. Steiner

Agricultural Research Magazine

Native juniper trees and invasive plants pose an expanding threat to the survival of the sagebrush ecosystem in arid western rangelands. As the trees mature, they compete with other native plants that are valuable food sources for wildlife and cattle. After these plants die back, the bare patches of soil that remain are vulnerable to erosion and encroachment from other species like cheatgrass, an invasive nonnative annual that fuels wildfires.

Wildfires threaten livestock ranches, are suppressed in large part by efforts funded with taxpayer dollars, and increase the probability that invasive plants will spread and thrive in the postfire landscape. …

Better French Fries: Technique Helps Lower Fat, Zhongli Pan, Marcia Wood

Agricultural Research Magazine

No doubt about it. Americans love French fries.

But fries soak up a lot of oil while they’re turning a perfect golden brown in the deep-fat fryer.

Agricultural Research Service scientist Zhongli Pan and colleagues have tackled the challenge of lowering some of the fat from this favorite side order without skimping on the fries’ delicious taste and texture or their pleasing appearance and aroma. Their idea? After potatoes are peeled and sliced into strips, and just before the raw strips are dunked in the fryer, prep them with 3 minutes of infrared (IR) heat—just like that created in home …

Fungi Can Be Friends Or Foes, Amy Rossman, Jo Anne Crouch, Shannon Dominick, Sharon Durham

Agricultural Research Magazine

Fungi are a large and diverse group of organisms. Some fungi, like mushrooms, are edible and considered crops. Others, however, can cause serious diseases of crop and forest plants, and those diseases can have negative effects on local and international economies as well as on the supply of food and other materials that agriculture provides.

The Agricultural Research Service maintains a unique resource—the U.S. National Fungus Collections—for helpful and detailed information about fungi. Accurate knowledge of fungi is critical for controlling the diseases they cause.

North America’s Largest Fungarium

In 1869, the Smithsonian Institution transferred its fungal collection to the …

Measuring The Feeding Behavior Of Livestock, T. M. Brown-Brandl, Roger A. Eigenberg, Sandra Avant

Agricultural Research Magazine

Determining how much time animals spend eating could help animal caretakers identify sick livestock, improve management, and establish genetic differences within a herd. But first, a system is needed to monitor animal feeding behavior.

Scientists at the Agricultural Research Service’s Roman L. Hruska U.S. Meat Animal Research Center in Clay Center, Nebraska, have developed a new system to monitor feeding behavior of feedlot cattle and grow-finish swine in a livestock industry setting.

The system, created by agricultural engineers Tami Brown-Brandl and Roger Eigenberg in the center’s Environmental Management Research Unit, uses standard radio-frequency identification technology designed around a commercial reader. …

Agricultural Research Magazine, July 2013

Agricultural Research Magazine

Table of Contents

4 Turning Trees Into Fighter Fuels

8 Tools for Tracking Troublesome Trees

10 Better Maps Mean Better Rangeland Management

12 New, Improved Codling Moth Lures Available

13 New Strawberry Species Found in Oregon

14 Measuring the Feeding Behavior of Livestock

15 Savvy Seed Sorter Gains New Fans

16 Fungi Can Be Friends or Foes

18 Detecting Deadly Colonies of E. coli

20 Storage Technique Preserves Citrus, Kills Pathogens

22 Better French Fries: Technique Helps Lower Fat

23 Locations Featured in This Magazine Issue

July 2013- Locations Featured In This Magazine Issue

Agricultural Research Magazine

The Agricultural Research Service has about 100 labs all over the country.

Locations Featured in This Magazine Issue

Albany, California 9 research units ■ 241 employees

Corvallis, Oregon 3 research units ■ 127 employees

Great Basin Rangelands Research Unit, Reno, Nevada 1 research unit ■ 19 employees

U.S. Salinity Laboratory, Riverside, California 2 research units ■ 39 employees

Yakima Agricultural Research Laboratory, Wapato, Washington 1 research unit ■ 57 employees

Eastern Oregon Agricultural Research Center, Burns, Oregon 1 research unit ■ 27 employees

Northwest Watershed Research Center, Boise, Idaho 1 research unit ■ 18 employees

Tucson, Arizona 2 research units …

New, Improved Codling Moth Lures Available, David Nicholson, Jan Suszkiw

Agricultural Research Magazine

New lures that entice codling moths with the scent of food and a possible mate are available for use in monitoring this orchard pest and controlling it with carefully timed applications of insecticide. The research related to the development and use of the lures involved scientists Alan Knight and Peter Landolt at the Agricultural Research Service’s Yakima Agricultural Research Laboratory in Wapato, Washington, and Douglas Light at the ARS Western Regional Research Center in Albany, California.

As larvae, codling moths are major pests of apples, pears, and walnuts. Historically, growers have sprayed their orchards with insecticide to prevent the pinkish-white, …

Better Maps Mean Better Rangeland Management, Brandon Bestelmeyer, Ann Perry

Agricultural Research Magazine

Land managers are always hoping for the next best thing to help them figure out where they should spend their time and money restoring and maintaining healthy rangelands. Now Agricultural Research Service rangeland ecologist Brandon Bestelmeyer has one of the answers—an ecological-state map that identifies where rangeland is holding its own, where it could respond to restoration efforts, or where it’s already past the point of no return.

“We wanted to find a way to turn existing field-level rangeland assessments into broader tools for comprehensively managing larger landscapes,” says Bestelmeyer, who works at the ARS Jornada Experimental Range in Las …

New Strawberry Species Found In Oregon, Kim E. Hummer, Sharon Durham

Agricultural Research Magazine

A recently discovered wild strawberry species provides new genetic material for plant research and, in the future, might also provide a new class of commercial strawberries.

Agricultural Research Service scientist Kim Hummer, with the USDA-ARS National Clonal Germplasm Repository at Corvallis, Oregon, found the new species during several plant collection expeditions in the high peaks of Oregon’s Cascade Mountains. She named it Fragaria cascadensis.

The find was reported in the Journal of the Botanical Research Institute of Texas.

The new strawberry is endemic to the Oregon Cascades, hence its specific name, F. cascadensis. It is perennial, with …

Turning Trees Into Fighter Fuels, Ann Perry

Agricultural Research Magazine

In western U.S. rangelands, native juniper and pinyon pine trees are spreading beyond their historical ecological niches and disrupting the environmental balance of their expanded range. Meanwhile, Agricultural Research Service scientists are teaming with university and industry colleagues to turn this problem into a source of fuel for U.S. Navy fighter jets.

“Juniper competes with grass and forbs for water and nutrients, and this leaves bare soil that is vulnerable to erosion,” says ARS scientist Mark Weltz, who works at the Great Basin Rangelands Research Unit in Reno, Nevada. “We have also lost habitat for sage grouse and mule deer, …

Tools For Tracking Troublesome Trees, Kirk Davies, Matt Madsen, Ann Perry

Agricultural Research Magazine

In Oregon, western juniper trees are expanding their range, pushing out other plant species, reducing sagebrush habitat and livestock forage, and at times fueling catastrophic wildfires. During some of these conflagrations, fires burn even hotter because more wood is available to feed the flames—a cause-and-effect that in turn lengthens the time that temperatures remain elevated at fire-stricken sites.

To help streamline efforts to manage invasive trees, Agricultural Research Service rangeland scientists Kirk Davies and Matt Madsen are investigating ways of combining aerial photography and computer programs to quickly identify and measure affected areas. These tools could save time and money …

An Hsus Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus), The Humane Society Of The United States

Impact of Animal Agriculture

Staphylococcus aureus is a leading cause of bacterial infection and is increasingly found to be resistant to antibiotic therapy. A newly described type of Methicillin Resistant Staphylococcus aureus carried by farm animals, Livestock Associated MRSA (LA-MRSA), is now causing infections in humans with and without direct livestock contact. A reduction in the non-therapeutic use of antibiotics in feed would likely reduce the capacity of industrial animal agriculture to continue to create, disseminate, and perpetuate a large reservoir of LA-MRSA on a global scale, but more fundamental changes in the way animals are raised for food may be necessary forestall a …

Savvy Seed Sorter Gains New Fans, Thomas C. Pearson, Marcia Wood

Agricultural Research Magazine

Simple, swift, and comparatively inexpensive, a color-image-based seed sorter is helping plant breeders and others separate the seeds they want from those they don’t—with an impressive degree of accuracy.

Agricultural Research Service agricultural engineer Thomas C. Pearson, based at the agency’s Center for Grain and Animal Health Research in Manhattan, Kansas, developed the sorter in collaboration with National Manufacturing in Lincoln, Nebraska. The company has marketed the device to customers in the United States and abroad since 2010.

In tests, the compact, portable sorter— a simpler and faster version of other machine-vision equipment that Pearson developed in 2009—speedily differentiated kernels …

Another Comic Food Song: The Irish Jubilee, Máirtín Mac Con Iomaire

Articles

This is a comic food song from the Irish American tradition.

Kinetics Of Tomato Peroxidase Inactivation By Atmospheric Pressure Cold Plasma Based On Dielectric Barrier Discharge, Patrick J. Cullen, Shashi Pankaj, N. Misra

Articles

Atmospheric pressure cold plasma technology is an emerging nonthermal food technology for microbiological decontamination of food and bio-materials. This study demonstrates the applicability of in-package cold plasma technology as a novel means to inactivation of enzymes. The kinetics of inactivation of tomato peroxidase as a model enzyme was studied at 30, 40 and 50kV, for up to 5’ of atmospheric air dielectric barrier discharge plasma treatments. The enzyme activity was found to decrease with both treatment time and voltage, the former variable exhibiting a more pronounced effect. Kinetic models viz. first-order, Weibull and logistic models were fitted to the experimentally …

Growing South Dakota (Summer 2013), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Fish, Field & Forest: Natural Resource Management Research Covers the Gamut
[Page] 5 Profiles In Leadership: David Willis; Combined Department Creates Interdisciplinary Opportunities
[Page] 6 Research On the Range: Cottonwood Field Station Has Provided Research Data for 106 Years
[Page] 8 Prairie Oasis: Natural Diversity Showcased at Oak Lake Field Station
[Page] 9 Special Section: Investigating in Agriculture & Biological Sciences at SDSU: An Update On the College of Ag’s Enhanced Facilities and Continuing Efforts Important to Helping Address Future Agricultural Challenges and Opportunities.[Page] 13 South Dakota 4-H Update: Skill-a-thons, New Exhibit Hall Planned
[Page] 14 After The …

Field Evaluation Of Arbuscular Mycorrhizal Fungal Colonization In Bacillus Thuringiensis Toxin-Expressing (Bt) And Non-Bt Maize, Tanya E. Cheeke, Mitchell B. Cruzan, Todd N. Rosenstiel

Biology Faculty Publications and Presentations

The cultivation of genetically engineered Bacillus thuringiensis toxin-expressing (Bt) maize continues to increase worldwide, yet the effects of Bt crops on arbuscular mycorrhizal fungi (AMF) in soil are poorly understood. In this field experiment, we investigated the impact of seven different genotypes of Bt maize and five corresponding non-Bt parental cultivars on AMF and evaluated plant growth responses at three different physiological time points. Plants were harvested 60 days (active growth), 90 days (tasseling and starting to produce ears), and 130 days (maturity) after sowing, and data on plant growth responses and percent AMF colonization of roots at each harvest …

Characterization Of The Gut Microbiota And Colitogenic Bacterial Species In Core 1 O-Glycans Deficient Mice, Maria E. Perez-Munoz

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Mucus is one important component of the intestinal mucosal barrier, and loss of its functionality is associated with colitis in humans and mice. Mice deficient in core 1 O-glycans (TM-IEC C1galt1^-/-) display a thinner mucus layer upon mutation of the glycosyltransferase enzyme (C1GALT1) responsible for core 1 O-glycans attachment, thus constituting an experimental model for human Ulcerative Colitis (UC). Under conventional conditions, these mice experience spontaneous inflammation of the colon. However, the exact role of the microbiota in colitis development in these mice has not been systematically investigated.

Aimed to gain insights into the role of …

Towards A Structured Approach To Reading Historic Cookbooks, Máirtín Mac Con Iomaire

Articles

This article discusses the methodology Barbara Ketchum Wheaton uses in her Reading historic Cookbook Seminars in Harvard and elsewhere and discusses from a participants perspective the strenghts of using this methodology to unlock hidden secrets of historic cookbooks.

Food Security And State: Policy Considerations For The Contemporary Food Crisis, Mangala Subramaniam, Christopher Bunka

Purdue Policy Research Institute (PPRI) Policy Briefs

In 1996, The World Food Summit (WFS) set a target to eradicate hunger in all countries and an immediate goal to half the number of undernourished people by 2015. Backed by the United Nations (UN), international organizations launched a global effort with the intent of achieving food security for all people. A variety of approaches were employed, including the distribution of food aid and farming supplies, skills training in agricultural development, funding for country-specific research, and legal counsel for states. Despite international efforts, over a decade later the number of undernourished was calculated to have risen by nearly 60 million …

Effect Of Enrichment-Bleaching And Low Oxygen Atmosphere Storage On All-Purpose Wheat Flour Quality, Jonathan Myers Swindler

Theses and Dissertations

All-purpose wheat flour is a useful long-term storage commodity, but is subject to off-odor formation. Although flour stored in a low oxygen atmosphere should inhibit rancid odor formation, it elicits consumer complaints about odor. The purpose of this study was to examine off-odor development in all-purpose wheat flour during ambient and elevated storage by determining the effect of low oxygen atmosphere and enrichment-bleaching on quality as measured by, free fatty acids (FFA), flour descriptive sensory analysis, conjugated dienes, headspace volatiles, bread consumer sensory analysis, color, loaf volume, and vitamin analysis. Enriched, bleached (EB) and unenriched, unbleached (UU) flour was stored …