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2013

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Full-Text Articles in Food Science

The Chemistry Of Flavonoids In Model Beverages And Human Milk, Brian Jay Song Oct 2013

The Chemistry Of Flavonoids In Model Beverages And Human Milk, Brian Jay Song

Open Access Dissertations

Interest in the chemistry and stability of flavonoids in foods has been generated by countless epidemiological, in vitro, and in vivo studies that suggest a variety of potential health promoting effects of diets rich in flavonoids. Flavonoids have been shown to interact with multiple components in beverages including macromolecules, minerals, certain vitamins, and other flavonoids. Furthermore, environmental conditions including light exposure, elevated temperatures, and relative humidity have been shown to modify flavonoid stability. However, specific mechanisms, relative kinetics of degradation reactions and the impact of macromolecules in model beverages and biological fluids on flavonoid stability remain largely unknown.

Recently, interest …


Invasive Silver Carp (Hypophthalmichthys Molitrix) Protein Hydrolysates- A Potential Source Of Natural Antioxidant, Sravanthi Priya Malaypally Oct 2013

Invasive Silver Carp (Hypophthalmichthys Molitrix) Protein Hydrolysates- A Potential Source Of Natural Antioxidant, Sravanthi Priya Malaypally

Open Access Dissertations

Invasive silver carp (Hypophthalmichthys molitrix), continue to spread over the Mississippi River causing a great concern for the river ecosystem due to their impact on native fish species. To minimize the negative effects of silver carp, many strategies were implemented including using it for animal feed, as fertilizers or simply discarding them into waste. However, these fish are high in protein content, making them excellent starting material for protein-derived by-products. One alternative is to recover and modify the protein in the fish into bioactive food ingredient. In the functional/ nutraceutical food industry, there is high demand for antioxidant agents. In …


Using X-Ray Imaging Techniques To Determine Density Of Foods, Shivangi Mohan Kelkar Oct 2013

Using X-Ray Imaging Techniques To Determine Density Of Foods, Shivangi Mohan Kelkar

Open Access Dissertations

Density depends on the experimental technique and structural properties of food. True, apparent and bulk are different types of densities. For porous foods such as baked food products and complex food systems, accurate measurement of density is challenging. Empirical correlations dependent on composition and temperature exist to calculate true density. Thus if composition and apparent density are known, porosity can be calculated, porosity = [1 - (apparent density/ true density)]. Various non-destructive diagnostic tools have been employed to study food microstructure. Among these, computed tomography (CT) and digital radiography (DR) utilizing x-ray imaging showed greater capability for evaluation of food …


Brain Bioavailability Of Polyphenols: Implications For Delivery Of Brain Health Benefits, Tzu-Ying Chen Oct 2013

Brain Bioavailability Of Polyphenols: Implications For Delivery Of Brain Health Benefits, Tzu-Ying Chen

Open Access Dissertations

Consumption of fruits and vegetables has been associated with neuroprotection and cognitive benefits throughout the life span. These associations have sparked interest in plant-derived polyphenols as biologically active agents with potential for targeting brain benefits. However, little is known regarding the ability of the polyphenols or their metabolites from polyphenol-rich products to cross the blood-brain-barrier, and be available for biological action. Furthermore, additional insight is needed on factors affecting the absorption and brain distribution of polyphenol metabolites in vivo. To fill gap in current knowledge, this thesis will focus on the effects of diabetogenic diet and diabetic state which have …


Retention Of Structure, Antigenicity, And Biological Function Of Pneumococcal Surface Protein A (Pspa) Released From Polyanhydride Nanoparticles, Shannon L. Haughney, Latrisha K. Petersen, Amy D. Schoofs, Amanda Ramer-Tait, Janice King, David Briles, Michael J. Wannemuehler, Balaji Narasimhan Sep 2013

Retention Of Structure, Antigenicity, And Biological Function Of Pneumococcal Surface Protein A (Pspa) Released From Polyanhydride Nanoparticles, Shannon L. Haughney, Latrisha K. Petersen, Amy D. Schoofs, Amanda Ramer-Tait, Janice King, David Briles, Michael J. Wannemuehler, Balaji Narasimhan

Department of Food Science and Technology: Faculty Publications

Pneumococcal surface protein A (PspA) is a choline-binding protein which is a virulence factor found on the surface of all Streptococcus pneumoniae strains. Vaccination with PspA has been shown to be protective against a lethal challenge with S. pneumoniae, making it a promising immunogen for use in vaccines. Herein, the design of a PspA-based subunit vaccine using polyanhydride nanoparticles as a delivery platform is described. Nanoparticles based on sebacic acid (SA), 1,6-bis-(p-carboxyphenoxy)hexane (CPH) and 1,8-bis-(p-carboxyphenoxy)-3,6- dioxaoctane (CPTEG), specifically 50:50 CPTEG:CPH and 20:80 CPH:SA, were used to encapsulate and release PspA. The protein released from the nanoparticle …


Food, Parasites, And Epidemiological Transitions: A Broad Perspective, Karl Reinhard, Luis Fernando Ferreira, Françoise Bouchet, L. Sianto, J. M.F. Dutra, A. Iniguez, Daniela Leles, M. Le Bailley, Martín Horacio Fugassa, Elisa Pucu, Adauto Araújo Sep 2013

Food, Parasites, And Epidemiological Transitions: A Broad Perspective, Karl Reinhard, Luis Fernando Ferreira, Françoise Bouchet, L. Sianto, J. M.F. Dutra, A. Iniguez, Daniela Leles, M. Le Bailley, Martín Horacio Fugassa, Elisa Pucu, Adauto Araújo

Karl Reinhard Publications

Pathoecology provides unique frameworks for understanding disease transmission in ancient populations. Analyses of Old and New World archaeological samples contribute empirically to our understanding of parasite infections. Combining archaeological and anthropological data, we gain insights about health, disease, and the way ancient people lived and interacted with each other and with their environments. Here we present Old and New World parasite evidence, emphasizing how such information reflects the different ways ancient populations exploited diverse environments and became infected with zoonotic parasites. It is clear that the most common intestinal helminthes (worm endoparasites) were already infecting ancient inhabitants of the New …


Wanda Grant, Wanda Grant, Institute Of Child Nutrition. Child Nutrition Archives. Aug 2013

Wanda Grant, Wanda Grant, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Wanda Grant is a retired School Nutrition Director with over 35 years of experience. She holds a Bachelor’s degree in Food, Nutrition and Institutional Management from Washington State University and is a registered dietitian. Her career took her from Washington to Alaska and finally to California. She is a credentialed School Nutrition Specialist with the School Nutrition Association (SNA), has served on the Executive Board of SNA, and was honored nationally in 2009 as Outstanding Director of the Year. Wanda is a certified ServSafe® instructor and a consultant trainer for the National Food Service Management Institute.


Back Matter Agricultural Research Magazine August 2013 Aug 2013

Back Matter Agricultural Research Magazine August 2013

Agricultural Research Magazine

U.S. Department of Agriculture

Agricultural Research Magazine

5601 Sunnyside Ave.

Beltsville, MD 20705-5128


Prescription For Curing Citrus Greening, Dennis O’Brien, Yongping Duan Aug 2013

Prescription For Curing Citrus Greening, Dennis O’Brien, Yongping Duan

Agricultural Research Magazine

Huanglongbing (HLB), or citrus greening, is the most serious threat to the Florida citrus industry in its history and is costing millions of dollars each year. Working with the Florida Department of Agriculture and Consumer Services (FDACS), Yongping Duan and his Agricultural Research Service colleagues in Fort Pierce, Florida, have found a formula for curbing HLB, offering the industry a glimmer of hope.

Duan has published details of work showing that heating potted citrus seedlings in greenhouses kills off the HLB bacterium and can rid the seedlings of citrus greening symptoms. Monitoring efforts show that the benefit can last for …


Early Weaning: A Good Bet For Beef Producers In Drought-Stricken Areas, R.C. Waterman, Sandra Avant Aug 2013

Early Weaning: A Good Bet For Beef Producers In Drought-Stricken Areas, R.C. Waterman, Sandra Avant

Agricultural Research Magazine

At the beginning of 2013, cattle-ranchers in the Northern Plains were among U.S. agricultural producers still feeling the lingering effects of the sweltering 2012 drought, the worst in half a century.

Beef cow numbers were at the lowest in 50 years as U.S. beef producers—severely affected by extended drought—tried to recover from some of the driest months on record.

Across the Northern and Southern Plains, beef producers hit the hardest by drought are threatened by limited forage resources for cows, which restricts calf growth, resulting in lighter calf weaning weights. In addition, drought can decrease cow body weight and condition …


Scientific Works Of Art Reveal A Hidden World, Gary Bauchan, Jan Suszkiw Aug 2013

Scientific Works Of Art Reveal A Hidden World, Gary Bauchan, Jan Suszkiw

Agricultural Research Magazine

It has been said that a picture is worth a thousand words, and at the Agricultural Research Service’s Electron and Confocal Microscopy Unit (ECMU) in Beltsville, Maryland, this adage couldn’t be more true. Led by unit director Gary Bauchan, the ECMU is tasked with producing highresolution images that provide a window to the extraordinary world of the unseen.

“We have observed viruses, bacteria, fungi, nematodes, insects, mites, and parasites that threaten global food security, and we’ve contributed to the discovery of how pathogens spread by helping elucidate their relationship to the environment, hosts, and vectors,” says Bauchan. “We’ve also described …


Novel Ways To Combat Plant Pathogens, Deborah Fravel, Gail C. Wisler Aug 2013

Novel Ways To Combat Plant Pathogens, Deborah Fravel, Gail C. Wisler

Agricultural Research Magazine

Plant diseases must be managed to successfully and reliably produce crops to meet humanity’s growing food, fiber, feed, and fuel needs. Plant disease management relies on many different types of tools—from disease-resistant plant varieties and pesticides to cultural and biological strategies. Agricultural Research Service scientists are seeking new methods of managing plant diseases, more efficient means of using older methods, and combinations of these methods. Two of the oldest ways of reducing plant diseases are crop rotation and organic amendments to soil.

In Orono, Maine, for example, ARS scientists are evaluating a holistic approach to suppressing soilborne pathogens of potato, …


Possible Links Between Soil Microbial Communities And Stroke Risk, Ann Perry Aug 2013

Possible Links Between Soil Microbial Communities And Stroke Risk, Ann Perry

Agricultural Research Magazine

“A big part of our Agricultural Research Service culture is cross-talking with outside researchers,” says ARS soil and water scientist Patrick Hunt. “Partnerships are what we do.”

So one day in 2011, Hunt called Medical University of South Carolina professor Daniel Lackland to discuss a paper Lackland had published about stroke risk in the state. South Carolina is part of the U.S. “Stroke Belt,” where residents have a significantly higher incidence of stroke than the rest of the U.S. population. South Carolina counties with the highest rates of stroke—between 89 and 115 cases per 100,000 residents—are found within the Southern …


Fungal Foam Tested Against Avocado Threat, Jan Suszkiw Aug 2013

Fungal Foam Tested Against Avocado Threat, Jan Suszkiw

Agricultural Research Magazine

Avocados aren’t just nutritional powerhouses; they’re also the chief ingredient in such party favorites as guacamole dip.

More than 99 percent of the nation’s $322 million avocado crop is grown in south Florida and southern California (less than 1 percent is produced in Hawaii), which makes recent infestations of groves there by invasive, wood-boring ambrosia beetles so alarming. A host of counter strategies are in the works, including a biobased foam originally developed by Agricultural Research Service scientists for use against Formosan subterranean termites.

In Miami-Dade County, Florida, avocado growers are contending with Xyleborus glabratus, the redbay ambrosia beetle. …


Fire Ant Venom Compounds May Be Useful As A Fungicide, Jan Suszkiw, Jian Chen, Xixuan Jin Aug 2013

Fire Ant Venom Compounds May Be Useful As A Fungicide, Jan Suszkiw, Jian Chen, Xixuan Jin

Agricultural Research Magazine

Red imported fire ants are named for the firelike burn of their sting. Now, the same venom that packs such a painful wallop may actually do some good for a change.

Studies by scientists at the Agricultural Research Service’s Biological Control of Pests Research Unit in Stoneville, Mississippi, have shown that certain alkaloid compounds in the venom—namely, piperideines and piperidines—can hinder growth of Pythium ultimum, a top crop pathogen worldwide.

Chemical controls, delayed plantings, and crop rotation are among methods now used against P. ultimum, which causes damping-off diseases that decay the seed or seedlings of vegetable, horticultural, …


August 2013- Locations Featured In This Magazine Issue Aug 2013

August 2013- Locations Featured In This Magazine Issue

Agricultural Research Magazine

Albany, California

9 research units ■ 241 employees

San Joaquin Valley Agricultural Sciences Center, Parlier, California

3 research units ■ 125 employees

Northwest Watershed Research Center, Boise, Idaho

1 research unit ■ 18 employees

Tucson, Arizona

2 research units ■ 54 employees

U.S. Sheep Experiment Station, Dubois, Idaho

1 research unit ■ 10 employees

Fort Keogh Livestock and Range Research Laboratory, Miles City, Montana

1 research unit ■ 25 employees

Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska

6 research units ■ 117 employees

Grazinglands Research Laboratory, El Reno, Oklahoma

2 research units ■ 43 employees

National …


Ars Validates Soil Moisture Data Collected Via Satellite, Ann Perry Aug 2013

Ars Validates Soil Moisture Data Collected Via Satellite, Ann Perry

Agricultural Research Magazine

The European Space Agency’s Soil Moisture and Ocean Salinity (SMOS) mission includes the latest advance in using Earth-orbiting satellites for estimating soil moisture across the globe. The SMOS satellite was launched in 2009 and was designed to estimate soil moisture levels to within 4 percent, which is like measuring a teaspoon of water in a handful of dry soil.

To capture this data, SMOS uses a new sensor technology that is the first passive L-band system—measuring microwave radiation emitted around the frequency of 1.4 gigahertz—in routine operation. But the accuracy of the information collected by this new technology still needs …


New Strategies To Thwart Pecan Scab, Sharon Durham Aug 2013

New Strategies To Thwart Pecan Scab, Sharon Durham

Agricultural Research Magazine

Pecans are great for eating out of the shell or in a myriad of recipes. But abundant pickings of high-quality nuts are only possible if the tree escapes the devastating disease called “pecan scab.” Caused by the fungus Fusicladium effusum, it is the most destructive disease of pecan in the southeastern United States. When scab is severe, most often when rainfall is above average, nut size is reduced, and total crop loss might occur.

Scientists at the Southeastern Fruit and Tree Nut Research Laboratory in Byron, Georgia, are working to help pecan growers mitigate the effect of pecan scab. …


Screening Fresh Oranges With Uv Study Pinpoints New Value Of Detection Tactic, David M. Obenland, Joseph L. Smilanick, Marcia Wood Aug 2013

Screening Fresh Oranges With Uv Study Pinpoints New Value Of Detection Tactic, David M. Obenland, Joseph L. Smilanick, Marcia Wood

Agricultural Research Magazine

Fresh, deliciously sweet navel oranges, on display at your local supermarket, may have been quickly inspected with ultraviolet (UV) light when they were still at the packinghouse. Usually, the purpose of this special sorting and screening is to see if circular spots—which glow a bright, fluorescent yellow and may be about the size of a quarter or larger—show up on the fruit’s peel.

More often than not, these spots, which scientists refer to as “lesions,” are telltale indicators of the presence of microbes that cause decay, namely Penicillium italicum, responsible for blue mold, or P. digitatum, the culprit …


Agricultural Research Magazine August 2013 Aug 2013

Agricultural Research Magazine August 2013

Agricultural Research Magazine

Table of Contents

4 Prescription for Curing Citrus Greening: Apply Heat and Wait

7 Screening Fresh Oranges With UV: Study Pinpoints New Value of Detection Tactic

8 Breed Matters: Selecting Rams for Rangeland Production

10 Scientific Works of Art Reveal a Hidden World

13 ARS Validates Soil Moisture Data Collected Via Satellite

14 New Strategies To Thwart Pecan Scab

15 Fire Ant Venom Compounds May Be Useful as a Fungicide

16 Possible Links Between Soil Microbial Communities and Stroke Risk

18 Early Weaning: A Good Bet for Beef Producers in Drought-Stricken Areas

20 Fungal Foam Tested Against Avocado Threat

21 …


Effects Of Composition And Separation Techniques On The Structure Of Aggregates In The Milk Fat Globular Membrane, Tristan Dilbeck, Jose Ibarra, Rafael Jimenez-Flores, Tracey Nguyen Aug 2013

Effects Of Composition And Separation Techniques On The Structure Of Aggregates In The Milk Fat Globular Membrane, Tristan Dilbeck, Jose Ibarra, Rafael Jimenez-Flores, Tracey Nguyen

STAR Program Research Presentations

The milk fat globular membrane (MFGM) is a complex structure composed mainly of phospholipids, sphingolipids, and membrane specific proteins. The composition and size of the MFGM differs based on the isolation, purification, and techniques used in analysis of the membrane as well as physiological, chemical, and mechanical factors present during its creation. The purpose of this experiment is to analyze the effects of separation techniques and composition of lipids and proteins on the structure of aggregates in the MFGM. The aggregates found in whey protein isolate (95% pure whey protein), whey buttermilk (whey protein and phospholipids), and sweet buttermilk (whey …


The Effects Of Microfluidization And Homogenization On The Composition And Structure Of Liposomal Aggregates From Whey Buttermilk And Commercial Buttermilk, Tracey Mai T. Nguyen Aug 2013

The Effects Of Microfluidization And Homogenization On The Composition And Structure Of Liposomal Aggregates From Whey Buttermilk And Commercial Buttermilk, Tracey Mai T. Nguyen

Master's Theses

Milk derived ingredients from the production of cheese and butter can be used as vehicles for nutrients. Buttermilk is a nutritious product of milk that comes from the churning of cream into butter. One of the advantages of buttermilk is that it is enriched in milk fat globule components, such as phospholipids and forms emulsions with fat when treated with high shear. The objective of this work was to explore the effects of shear on regular buttermilk and whey buttermilk in terms of liposomal aggregate size and chemical composition. The effects of microfluidization at 2000 psi and homogenization at 2000 …


Characterization Of Bacillus Bacterial Strains; Optimum Growth Temperature, Proteolytic And Lipolytic Activity, Jamie Anne Boring Aug 2013

Characterization Of Bacillus Bacterial Strains; Optimum Growth Temperature, Proteolytic And Lipolytic Activity, Jamie Anne Boring

STAR Program Research Presentations

Characterization of Bacillus bacterial strains is an important step in identifying and removing biofilms contaminating processing equipment. These biofilms lead to large amounts of spores in the final product. Strains that produce spoilage enzymes cause off-flavors, structural defects and reduced shelf life of milk products. Using four treatments, we determined the best growth protocol for each strain to use in future experiments. Treatment 1: 80 o C heat shock and 32 o C 24hr incubation, Treatment 2: 80o C/ 55 o C, Treatment 3: 100 o C/ 32 o C, Treatment 4: 100 o C/ 55 o C. Our …


Effects Of Xanthan/Locust Bean Gum Mixtures On The Physicochemical Properties And Oxidative Stability Of Whey Protein Stabilized Oil-In-Water Emulsions, Goutham Puli Aug 2013

Effects Of Xanthan/Locust Bean Gum Mixtures On The Physicochemical Properties And Oxidative Stability Of Whey Protein Stabilized Oil-In-Water Emulsions, Goutham Puli

Masters Theses & Specialist Projects

Scientific evidence shows that dietary intake of the omega-3 polyunsaturated fatty acids is beneficial to human health. Fish oil is a rich source of omega-3 fatty acids. However, fish oil with high levels of omega-3 PUFA is very susceptible to oxidative deterioration during storage. The objective of this study was to investigate the effect of xanthan gum (XG)-locust bean gum (LBG) mixtures on the physicochemical properties of whey protein isolate (WPI) stabilized oil-in-water (O/W) emulsions containing 20% v/v menhaden oil. The O/W emulsions containing XG/LBG mixtures were compared to emulsions with either XG or LBG alone. The emulsions were prepared …


Development Of Tools To Assess The Effects Of Lunasin On Normal Development And Tumor Progression In Drosophila Melanogaster, Gillian E. Jones Aug 2013

Development Of Tools To Assess The Effects Of Lunasin On Normal Development And Tumor Progression In Drosophila Melanogaster, Gillian E. Jones

Masters Theses & Specialist Projects

Soy contains many bioactive molecules known to elicit anti-cancer effects. One such peptide, Lunasin, has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. In this study we attempt to understand the developmental effects of Lunasin overexpression in vivo and create reagents that will help us understand Lunasin’s anti tumorigenic effects in an intact organism. cDNA encoding lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. …


Development Of Hplc Methods For The Determination Of Water-Soluble Vitamins In Pharmaceuticals And Fortified Food Products, Hung Khiem Trang Aug 2013

Development Of Hplc Methods For The Determination Of Water-Soluble Vitamins In Pharmaceuticals And Fortified Food Products, Hung Khiem Trang

All Theses

Though many HPLC methods have been developed and reported in literature for vitamin analysis for the past two decades, applying certain methods directly from literature more than often fails to reproduce the results reported due to many variables of liquid chromatography. This issue was targeted in this project through the examination of chromatographic behaviors of water-soluble vitamins in order to help the analysts better modify methods from literature or even develop new methods from scratch to fit their analytical need with the resources available (e.g., columns, detectors, etc.) in their lab.
The first part of the project investigated the chromatographic …


Kinetic Studies For The Preparation Of Probiotic Cabbage Juice: Impact On Phytochemicals And Bioactivity, Amit Jaiswal, Nissreen Abu-Ghannam Aug 2013

Kinetic Studies For The Preparation Of Probiotic Cabbage Juice: Impact On Phytochemicals And Bioactivity, Amit Jaiswal, Nissreen Abu-Ghannam

Articles

A kinetic study for the production of probiotic cabbage juice was carried out under controlled pH and dissolved oxygen using several strains of Lactic acid bacteria (LAB). Furthermore, effect of probiotic fermentation on polyphenolic content and antioxidant capacity was investigated. Results showed significant growth in Lactobacillus brevis, Lactobacillus plantarum and Lactobacillus rhamnosus corresponding to 9.19, 9.47 and 10.6 log CFU/ml after 24 h of fermentation, which is satisfying criteria for a food product to be characterized as probiotic. Lactic acid (LA) was the major end product of the fermented cabbage juice attaining the concentrations of 6.97, 9.69 and 12.2 g/l …


Excretion Of Phenolic Compounds After Consumption Of Fresh Versus Aged Chokeberry Juice In Rats, Sarah Elizabeth Graves Aug 2013

Excretion Of Phenolic Compounds After Consumption Of Fresh Versus Aged Chokeberry Juice In Rats, Sarah Elizabeth Graves

Graduate Theses and Dissertations

Flavonoids, such as anthocyanins, may be responsible for the health benefits of chokeberries, as they are present in large quantities in chokeberries and are potent antioxidant and anti-inflammatory agents. Despite these health properties, previous investigations on the bioavailability of flavonoids illustrate that little of the ingested amount reaches the bloodstream. This study investigated two factors that may help to explain these inconsistencies. First, storage of berry fruits and berry fruit products leads to extensive polymerization of their anthocyanins with procyanidins. Additionally, after consumption flavonoids may undergo metabolism into phenolic acids and subsequent conjugation to an extent that may explain their …


Influences Of Music Genre And Components On Food Perception And Acceptance, Alexandra Jean Fiegel Aug 2013

Influences Of Music Genre And Components On Food Perception And Acceptance, Alexandra Jean Fiegel

Graduate Theses and Dissertations

Managers of consumer goods companies (i.e., restaurants, grocery stores, and bars) have the potential to effectively utilize environmental factors to stimulate desired consumer behaviors. Background music has been identified as one of the most readily manipulated and influential elements to which a shopper or consumer may be exposed to in a service setting. Nevertheless, little is known about the effect of background sound on food perception and acceptance. This research sought whether background music genre and musical components can alter food perception and acceptance, but also to determine how the effect can vary as a function of food type (i.e., …


Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pecan Residues In Processed Foods, Denise Tran Aug 2013

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pecan Residues In Processed Foods, Denise Tran

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Pecan nuts are becoming increasingly popular due to their link to health benefits. However, the presence of undeclared pecan residues in food products can pose serious health risks for pecan-allergic consumers. Currently, analytical methods for the detection of pecan allergens are limited and have not been validated for use by the food industry to assess the effectiveness of allergen control programs. Therefore, the aim of this study was to develop a sandwich-type ELISA to detect and quantify allergenic pecan residues in processed foods. Several varieties of pecan were mixed, roasted, and ground to immunize a goat, sheep, and rabbits. The …