Food Science Commons^™

Electromagnetic And Heat Transfer Modeling Of Microwave Heating In Domestic Ovens, Krishnamoorthy Pitchai

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Microwave (MW) ovens are used extensively for heating a variety of not-ready-to-eat food products. It is vital to achieve target temperature uniformly throughout the food to inactivate foodborne pathogens to assure safety. Non-uniform heating of foods in microwave ovens is the major concern in assuring microbiological safety of such products. The non-uniform heating of foods in domestic microwave ovens is due to complex interactions of microwaves with foods. A comprehensive coupled electromagnetic and heat transfer model was developed using finite-difference time-domain based numerical method to understand the complex interaction of microwaves with foods. Simulation parameters such as cell size, heating …

Evaluation And Analysis Of Beef Contamination By Low Levels Of Ammonia, Sely Prajitna

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Leakage of ammonia in food facilities has been known to occur in the past. Ammonia leaks often lead to food contamination in food plants and can cause illness among consumers who accidentally consume the food products contaminated with high levels of ammonia. Therefore, a rapid, simple yet accurate method has to be established for on-site ammonia level screening in food plants to ensure the food is safe. One of the primary objectives in this study was to verify and optimize an ion selective electrode (ISE) method for its accuracy and reproducibility in determining ammonia in meat. Different extraction procedures (blending …

Design Of A Protective Single-Dose Intranasal Nanoparticle-Based Vaccine Platform For Respiratory Infectious Diseases, Bret D. Ulery, Devender Kumar, Amanda E. Ramer-Tait, Dennis W. Metzger, Michael J. Wannemeuhler, Balaji Narasimhan

Department of Food Science and Technology: Faculty Publications

Despite the successes provided by vaccination, many challenges still exist with respect to controlling new and re-emerging infectious diseases. Innovative vaccine platforms composed of adaptable adjuvants able to appropriately modulate immune responses, induce long-lived immunity in a single dose, and deliver immunogens in a safe and stable manner via multiple routes of administration are needed. This work describes the development of a novel biodegradable polyanhydride nanoparticle-based vaccine platform administered as a single intranasal dose that induced long-lived protective immunity against respiratory disease caused by Yesinia pestis, the causative agent of pneumonic plague. Relative to the responses induced by the …

Host-Microbial Symbiosis In The Vertebrate Gastrointestinal Tract And The Lactobacillus Reuteri Paradigm, Jens Walter, Robert A. Britton, Stefan Roos

Department of Food Science and Technology: Faculty Publications

Vertebrates engage in symbiotic associations with vast and complex microbial communities that colonize their gastrointestinal tracts. Recent advances have provided mechanistic insight into the important contributions of the gut microbiome to vertebrate biology, but questions remain about the evolutionary processes that have shaped symbiotic interactions in the gut and the consequences that arise for both the microbes and the host. Here we discuss the biological principles that underlie microbial symbiosis in the vertebrate gut and the potential of the development of mutualism. We then review phylogenetic and experimental studies on the vertebrate symbiont Lactobacillus reuteri that have provided novel insight …

Sorghum Clear Beer Value Chain, Bernadette C. Chimai

INTSORMIL Presentations

The markets and industrial utilization of agricultural commodities have undergone considerable change in the last few decades.

In Zambia, sorghum was considered a traditional crop with limited industrial uses.

Research and transformations in consumer tastes and preferences have led to the development of new uses for sorghum.

Potential markets for industrial utilization of sorghum have been identified as food processing, beer brewing, feed concentrates and energy production.

One of the success stories in industrial utilization of sorghum has been Eagle, a sorghum based clear beer produced by Zambian breweries.

The study reported in this paper aims to examine the main …

Characterization Of Igg And Ige Binding To Parvalbumin Derived From Commercially Important Fish Species, P. Lee, Julie A. Nordlee, S. J. Koppelman, J. L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Rationale: Parvalbumin is recognized as pan-allergen in fish and frog. However, previous studies demonstrated that the IgE- and IgG-binding patterns to parvalbumins vary depending on the fish species. We aimed to use 3 anti-parvalbumin IgG and human IgE to investigate the contributing factors for the binding differences.

Methods: Indirect enzyme-linked immunosorbent assay (ELISA) and IgG immunoblotting were used to determine the reactivity of the polyclonal anti-cod parvalbumin antibody and the commercially available, monoclonal anti-frog and anti-carp parvalbumin antibodies against raw muscle extracts of 25 fish species. Additionally, sera from 46 individuals with clinical history of fish allergy were analyzed for …

Nutrient Management Record Keeping Calendar, July 2011-December 2012, Leslie J. Johnson, Charles Shapiro, Charles Wortmann, Chris G. Henry, Larry Howard, Tamilee D. Nennich

Nebraska Cooperative Extension: Faculty and Staff Publications

Records for Nebraska Concentrated Animal Feeding Operations

Record the initials of the person performing the inspection each time. Check marks will not satisfy the record-keeping requirements.

Record any maintenance and/or repairs.

Correct all deficiencies within 30 days.

Nutritional And Anti-Nutritional Compositions Of Defatted Nebraska Hybrid Hazelnut Meal, Yixiang Xu, Milford Hanna

Department of Food Science and Technology: Faculty Publications

Defatted meal of hybrid hazelnuts may have potential food and value-added industrial applications. The proximate composition, mineral elements, amino acid profile and anti-nutritional factors (total phenolics, tannins, condensed tannins and phytates) of defatted meals of ten Nebraska hybrid hazelnut genotypes were investigated. Excluding the amino acid profile, the nutritional and anti-nutritional compositions of the meals differed significantly, with high protein, carbohydrate, neutral and acid detergent fibre concentrations and energy value. Mineral concentrations were higher than those previously reported. K was the most abundant mineral element, followed by P, Ca and Mg. Seven essential amino acids accounted for approximately 27.8% of …

Specialized Adaptation Of A Lactic Acid Bacterium To The Milk Environment: The Comparative Genomics Of Streptococcus Thermophilus Lmd-9, Yong Jun Goh, Caitlin Goin, Sarah O'Flaherty, Eric Altermann, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

Background: Streptococcus thermophilus represents the only species among the streptococci that has “Generally Regarded As Safe” status and that plays an economically important role in the fermentation of yogurt and cheeses. We conducted comparative genome analysis of S. thermophilus LMD-9 to identify unique gene features as well as features that contribute to its adaptation to the dairy environment. In addition, we investigated the transcriptome response of LMD-9 during growth in milk in the presence of Lactobacillus delbrueckii ssp. bulgaricus, a companion culture in yogurt fermentation, and during lytic bacteriophage infection.

Results: The S. thermophilus LMD-9 genome is …

Composition And Sensory Evaluation Of Popcorn Flake Polymorphisms For A Select Butterfly-Type Hybrid, Jess C. Sweley, Devin J. Rose, David S. Jackson

Department of Food Science and Technology: Faculty Publications

The objective of this study was to identify and characterize different popped popcorn flake shapes, or polymorphisms, arising from a yellow butterfly popcorn hybrid (YP-213), and then to determine the impact of popcorn flake shape on composition and sensory characteristics. Kernels were popped using a microwave oven and visually sorted into three different polymorphisms depending on whether the appendages were expanded unilaterally, bilaterally, or multilaterally. When popped, 9.0 ± 3.1%, 71.2 ± 5.9% and 12.3 ± 3.8% of kernels were expanded unilaterally, bilaterally, and multilaterally, respectively, while 7.6 ± 1.4% of kernels remained unpopped. Expansion volumes for unilaterally, bilaterally, and …

Environment And Hybrid Influences On Rapid-Visco-Analysis Flour Properties Of Food-Grade Grain Sorghum, Joni K. Griess, Stephen C. Mason, David S. Jackson, Tom D. Galusha, Jeffrey F. Pedersen, Muhammad Yaseen

Department of Food Science and Technology: Faculty Publications

Grain processors would benefi t from information about the production environment and the infl uences of the sorghum [Sorghum bicolor (L.) Moench] hybrid on food-grade fl our properties. The objective of this study was to determine the effects of environment and hybrid on rapid-visco-analysis (RVA) fl our properties of commercially available food-grade sorghum. A randomized complete block experiment was planted in 12 environments, which included the 2004 and 2005 growing seasons and irrigated and dryland water regimes in eastern, central, and west central Nebraska, and a dryland, low-N environment in eastern Nebraska. The environment accounted for 71–85% of the total …

Suggested Improvements For The Allergenicity Assessment Of Genetically Modified Plants Used In Foods, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

Genetically modified (GM) plants are increasingly used for food production and industrial applications. As the global population has surpassed 7 billion and per capita consumption rises, food production is challenged by loss of arable land, changing weather patterns, and evolving plant pests and disease. Previous gains in quantity and quality relied on natural or artificial breeding, random mutagenesis, increased pesticide and fertilizer use, and improved farming techniques, all without a formal safety evaluation. However, the direct introduction of novel genes raised questions regarding safety that are being addressed by an evaluation process that considers potential increases in the allergenicity, toxicity, …

Wild Buckwheat Is Unlikely To Pose A Risk To Buckwheat-Allergic Individuals, Julie A. Nordlee, Rakhi Panda, Joseph L. Baumert, Richard E. Goodman, Steve Taylor

Department of Food Science and Technology: Faculty Publications

Buckwheat (Fagopyrum esculentum) is a commonly allergenic food especially in Asia where buckwheat is more commonly consumed. Wild buckwheat (Polygonum convolvulus, recently changed to Fallopia convolvulus) is an annual weed prevalent in grain-growing areas of the United States. Wild buckwheat is not closely related to edible buckwheat although the seeds do have some physical resemblance. A large shipment of wheat into Japan was halted by the discovery of the adventitious presence of wild buckwheat seeds over possible concerns for buckwheat-allergic consumers. However, IgE-binding was not observed to an extract of wild buckwheat using sera from …

Dietary Selenium Affects Host Selenoproteome Expression By Influencing The Gut Microbiota, Marina V. Kasaikina, Marina A. Kravtsova, Byung Cheon Lee, Javier Seravalli, Daniel A. Peterson, Jens Walter, Ryan Legge, Andrew K. Benson, Dolph L. Hatfield, Vadim Gladyshev

Department of Food Science and Technology: Faculty Publications

Colonization of the gastrointestinal tract and composition of the microbiota may be influenced by components of the diet, including trace elements. To understand how selenium regulates the intestinal microflora, we used high-throughput sequencing to examine the composition of gut microbiota of mice maintained on selenium-deficient, selenium-sufficient, and selenium-enriched diets. The microbiota diversity increased as a result of selenium in the diet. Specific phylotypes showed differential effects of selenium, even within a genus, implying that selenium had unique effects across microbial taxa. Conventionalized germfree mice subjected to selenium diets gave similar results and showed an increased diversity of the bacterial population …

An Experimental Live Chimeric Porcine Circovirus 1-2a Vaccine Decreases Porcine Circovirus 2b Viremia When Administered Intramuscularly Or Orally In A Porcine Circovirus 2b And Porcine Reproductive And Respiratory Syndrome Virus Dual-Challenge Model, Tanja Opriessnig, João C. Gomes-Neto, Michelle Hermann, Hui-Gang Shen, Nathan M. Beach, Yaowei Huang, Patrick G. Halbur, Xiang-Jin Meng

Department of Food Science and Technology: Faculty Publications

Commercially available inactivated vaccines against porcine circovirus type 2 (PCV2) have been shown to be effective in reducing PCV2 viremia. Live-attenuated, orally administered vaccines are widely used in the swine industry for several pathogens because of their ease of use yet they are not currently available for PCV2 and efficacy. The aims of this study were to determine the efficacy of a live-attenuated chimeric PCV2vaccine inadual-challengemodelusingPCV2bandporcine reproductiveandrespiratorysyndrome virus (PRRSV) and to compare intramuscular (IM) and oral (PO) routes of vaccination. Eighty-three 2-week-old pigs were randomized into 12 treatment groups: four vaccinated IM, four vaccinated PO and four non-vaccinated (control) groups. …

Il-2 Limits Il-12 Enhanced Lymphocyte Proliferation During Leishmania Amazonensis Infection, Amanda E. Ramer-Tait, Christine A. Petersen, Douglas E. Jones

Department of Food Science and Technology: Faculty Publications

C3H mice infected with Leishmania amazonensis develop persistent, localized lesions with high parasite loads. During infection, memory/effector CD44^hiCD4⁺ T cells proliferate and produce IL-2, but do not polarize to a known effector phenotype. Previous studies have demonstrated IL-12 is insufficient to skew these antigen-responsive T cells to a functional Th1 response. To determine the mechanism of this IL-12 unresponsiveness, we used an in vitro assay of repeated antigen activation. Memory/effector CD44^hiCD4⁺ T cells did not increase proliferation in response to either IL-2 or IL-12, although these cytokines upregulated CD25 expression. Neutralization of IL-2 enhanced …

Glutaraldehyde-Modified Recombinant Fel D 1: A Hypoallergen With Negligible Biological Activity But Retained Immunogenicity, Serge A. Versteeg, Ingrid Bulder, Martin Himly, Toni M. Van Capel, R. Van Den Hout, Stef J. Koppelman, Esther C. De Jong, Fatima Ferreira, Ronald Van Ree

Department of Food Science and Technology: Faculty Publications

Background: Recombinant allergens are under investigation for replacing allergen extracts in immunotherapy. Site-directed mutagenesis has been suggested as a strategy to develop hypoallergenic molecules that will reduce the risk of side effects. For decades, chemically modified allergen extracts have been used for the same reason.

Aim: To evaluate whether glutaraldehyde modification is a good strategy to produce hypoallergenic recombinant allergens with retained immunogenicity.

Methods: Fel d 1 was cloned as a single construct linking both chains of the molecule and expressed in Escherichia coli and Pichia pastoris. After physicochemical purification, recombinant Fel d 1 (rFel d 1) was …

Evaluation And Comparison Of The Species-Specificity Of 3 Antiparvalbumin Igg Antibodies, Poi-Wah Lee, Julie A. Nordlee, Stef J. Koppelman, Joseph L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Parvalbumin is a pan-allergen in fish and frogs that triggers IgE-mediated reactions in fish-allergic individuals. Previous studies demonstrated that antibodies raised against fish and frog parvalbumins displayed varying specificity for different fish species, and thus, the applicability of these antibodies for potential use in immunoassays to detect fish residues were limited. We aimed to determine the specificity of 3 IgG antibodies for various fish species. Indirect enzyme-linked immunosorbent assay (ELISA) and IgG-immunoblotting were used to compare the reactivity of the polyclonal anticod parvalbumin antibody and the commercially available, monoclonal antifrog and monoclonal anticarp parvalbumin antibodies against raw muscle extracts of …

The Importance Of Sorghum Grain Colour And Hardness, And Their Causes And Measurement, Doreen Mwiita Hikeezi

INTSORMIL Presentations

Discusses sorghum grain color and kernel hardness, assessment methods, and the importance of each to the overall quality of the grain.

Industrial Sorghum Malting: Technologies That Can Be Used And Practical Considerations, Gyebi Duodu

INTSORMIL Presentations

What is malting?

– Germination of grain in moist air under controlled conditions

Objectives for opaque beer brewing

–Mobilization of endogenous enzymes of the grain

–Use of the endogenous enzymes to modify grain constituents for ready solubilisation

–Use of the endogenous enzymes to solubilise starchy adjunct during mashing

Market Development In Support Of Sorghum And Millet Farmers In Tanzania And Zambia, J. Mark Erbaugh, Emmanuel R. Mbiha, Fredy T.M. Kilima, Precious Hamukwala, Gelson Tembo, Donald W. Larson

INTSORMIL Presentations

Problem: Sorghum and millet productivity, production and consumption have been stagnant or declining for 20 years.

Challenge: How to increase the productivity and incomes of sorghum and millet farmers to improve food security and accelerate economic growth.

Solution: Better technology and linking producers to markets are important parts of the solution to the problem.

Objectives

Examine the effects of new production and marketing technology on sorghum and millet producers’ income and productivity in Tanzania and Zambia.

Identify better technologies, enhance market linkages between producers and buyers, improve quality and diversify utilization to ensure food security and increase farm income.

Conclusions: …

Sorghum Malting: Introduction, Objectives, And Scientific Principles, John R.N. Taylor

INTSORMIL Presentations

What is malting? It is limited germination of cereal grains in moist air under controlled conditions.

Why malt sorghum products?

Sorghum malt for home brewing

For brewing opaque beer, lager and stout

For “brewing” malt (malta) type non-alcoholic beverages

To produce malt extract for hot malted beverages,cereal and confectionery flavoring and coloring

For breakfast cereals

For “power flour”–enzyme active flour to “thin” infant porridges

Also explains the science of malting, and the importance of using high-quality grain.

Eagle Lager: A Sorghum Success Story On Many Levels, Ian Mackintosh

INTSORMIL Presentations

In 1999 in Uganda we were faced with a static but highly competitive market.

Needed to grow our market and to do this we needed to make beer cheaper

It was felt that a suitable local ingredient would reduce the cost of manufacture and stimulate local agriculture and the economy as well as provide opportunities for growth and profit for the company.

An extensive base line study of the Ugandan agricultural sector was undertaken to determine what was “out there.”

It was clear that there was enormous potential but little else.

Sorghum seemed the logical starting point because there was …

Improvements In Sorghum Milling Technologies, Martin M. Kebakile

INTSORMIL Presentations

Discusses in some detail various sorghum milling techniques.

Sorghum Malting, Luke Mugode

INTSORMIL Presentations

Malting is defined as the germination of grain in moist air under controlled conditions. Malting occurs in three stages: steeping (soaking), germination, and drying or kilning, then milling can take place. Malting changes the chemistry of the grain.

Virtues Of Sorghum: Utilization And Supply Chain Management, Lloyd W. Rooney

INTSORMIL Presentations

Discusses the consumer demands, value-added supply chain, strategy for value-added products, functionality of sorghums and millets, sorghum food use in Central America, plant breeding and improvement of grain quality, sorghum image, special sorghums as a particularly healthy food item, food utilization, improving sorghum quality, effect of molds, insects, and weathering on grain quality, and mycotoxins.

Breeding Sorghum Cultivars For Processing, Gary Perterson

INTSORMIL Presentations

Discusses the diversity of traits in sorghum and details some aspects of breeding techniques.

Mycotoxins In Grains – Causes, Prevention And Control, Hester F. Vismer

INTSORMIL Presentations

Discusses mycotoxins, their effect on grain, mitigation considerations, and resulting human toxicology.

Effects Of Malting And Fermentation On The Composition And Functionality Of Sorghum Flours, Onesmo N.O. Mella

INTSORMIL Presentations

Includes information about the sorghum market in Tanzania, and provides an overview of the chemical components present in sorghum grain and their effects on the nutritional qualities of the grain with respect to human consumption. Explains that malting and fermentation improve the nutritional profile of sorghum grain, and describes the methods used to test this hypothesis.

Developing Entrepreneurship, The Tanzania Experience: Sua-Intsormil Project, Joseph J. Mpagalile, Wenceslaus R. Ballegu

INTSORMIL Presentations

Project Title: Building a Sustainable Infrastructure for Product Development and Food Entrepreneur/Industry Technical Support: A Strategy to Promote Increased Use of Sorghum and Millet in East Africa.

Long Term Program Objectives: Development of successful entrepreneurial businesses that add-value to sorghum and millet such that:

~Farmers have an established outlet for cash sales of high-quality sorghum and millet

~Small businesses or cooperatives develop processing capabilities enabling the incorporation of sorghum and millet into a wide variety of food products

~Markets and market channels for sorghum and millet-based products develop.

Further develop research, extension and marketing expertise of National Agricultural Research Institutes …